mbf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbf1
Entrez Gene ID	39842
Full Name	multiprotein bridging factor 1
Synonyms	BcDNA:GH10148,BcDNA:GM01267,CG4143,Dmel\CG4143,MBF-1,MBF1,MRE4,Mbf-1,Mbf1
General protein information	Preferred Names multiprotein bridging factor 1 Names CG4143 gene product from transcript CG4143-RC CG4143-PA CG4143-PB CG4143-PC CG4143-PD CG4143-PE mRNA-like ncRNA in embryogenesis 4 mbf1-PA mbf1-PB mbf1-PC mbf1-PD mbf1-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73A9-73A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259867.1	NP_001246796.1	multiprotein bridging factor 1, isoform C
NM_001275023.1	NP_001261952.1	multiprotein bridging factor 1, isoform E
NM_079386.2	NP_524110.1	multiprotein bridging factor 1, isoform A
NM_168673.2	NP_730178.1	multiprotein bridging factor 1, isoform B
NM_001259866.1	NP_001246795.1	multiprotein bridging factor 1, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EDF1	XP_537793.4
Pan troglodytes (chimpanzee)	EDF1	XP_001163031.2
Gallus gallus (chicken)	EDF1	NP_001006203.1
Schizosaccharomyces pombe (fission yeast)	SPBC83.17	NP_595650.1
Oryza sativa (rice)	Os08g0366100	NP_001061647.1
Xenopus tropicalis (tropical clawed frog)	edf1	NP_001016686.1
Homo sapiens (human)	EDF1	NP_003783.1
Bos taurus (cattle)	EDF1	NP_001030384.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004990	XP_315094.1
Saccharomyces cerevisiae (baker's yeast)	MBF1	NP_014942.4
Kluyveromyces lactis	KLLA0F25014g	XP_456195.2
Eremothecium gossypii	AGOS_AFR526C	NP_986073.1
Arabidopsis thaliana (thale cress)	MBF1B	NP_191427.1
Arabidopsis thaliana (thale cress)	MBF1A	NP_565981.1
Rattus norvegicus (Norway rat)	Edf1	NP_001100027.1
	EDF1	XP_001092893.1
Mus musculus (house mouse)	Edf1	NP_067494.1
Danio rerio (zebrafish)	edf1	NP_957039.1
Drosophila melanogaster (fruit fly)	mbf1	NP_001261952.1
Caenorhabditis elegans (roundworm)	mbf-1	NP_502166.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

by preserving the redox-sensitive AP-1 activity, MBF1 provides an advantage during oxidative stress
Title: Coactivator MBF1 preserves the redox-dependent AP-1 activity during oxidative stress in Drosophila.

These data demonstrate a crucial role of MBF1 in the development of tracheae and central nervous system
Title: Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems.

The following mbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18712	NM_001259866.1 Latest version!	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18712	NM_001259867.1 Latest version!	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18712	NM_079386.2 Latest version!	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18712	NM_168673.2 Latest version!	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa21007	NM_001275023.1 Latest version!	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbf1 ( NM_001259867.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246796.1 Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18712
Clone ID Related Accession (Same CDS sequence)	NM_001259867.1 , NM_079386.2 , NM_168673.2 , NM_001259866.1
Accession Version	NM_001259867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multiprotein bridging factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246796.1
CDS	249..686
Translation	MSDWDSVTVLRKKAPKSSTLKTESAVNQARRQGVAVDTQQKYGAGTNKQHVTTKNTAKLDRETEELRHDKIPLDVGKLIQQGRQSKGLSQKDLATKICEKQQVVTDYEAGRGIPNNLILGKMERVLGIKLRGKERGQPIAPPGKK

Target ORF information:

RefSeq Version	NM_001259867.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259867.1

ORF Insert Sequence:

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbf1 ( NM_079386.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524110.1 Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18712
Clone ID Related Accession (Same CDS sequence)	NM_001259867.1 , NM_079386.2 , NM_168673.2 , NM_001259866.1
Accession Version	NM_079386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multiprotein bridging factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079386.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524110.1
CDS	109..546
Translation	MSDWDSVTVLRKKAPKSSTLKTESAVNQARRQGVAVDTQQKYGAGTNKQHVTTKNTAKLDRETEELRHDKIPLDVGKLIQQGRQSKGLSQKDLATKICEKQQVVTDYEAGRGIPNNLILGKMERVLGIKLRGKERGQPIAPPGKK

Target ORF information:

RefSeq Version	NM_079386.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079386.2

ORF Insert Sequence:

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbf1 ( NM_168673.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730178.1 Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18712
Clone ID Related Accession (Same CDS sequence)	NM_001259867.1 , NM_079386.2 , NM_168673.2 , NM_001259866.1
Accession Version	NM_168673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multiprotein bridging factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_168673.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730178.1
CDS	209..646
Translation	MSDWDSVTVLRKKAPKSSTLKTESAVNQARRQGVAVDTQQKYGAGTNKQHVTTKNTAKLDRETEELRHDKIPLDVGKLIQQGRQSKGLSQKDLATKICEKQQVVTDYEAGRGIPNNLILGKMERVLGIKLRGKERGQPIAPPGKK

Target ORF information:

RefSeq Version	NM_168673.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168673.2

ORF Insert Sequence:

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbf1 ( NM_001259866.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246795.1 Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18712
Clone ID Related Accession (Same CDS sequence)	NM_001259867.1 , NM_079386.2 , NM_168673.2 , NM_001259866.1
Accession Version	NM_001259866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multiprotein bridging factor 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246795.1
CDS	109..546
Translation	MSDWDSVTVLRKKAPKSSTLKTESAVNQARRQGVAVDTQQKYGAGTNKQHVTTKNTAKLDRETEELRHDKIPLDVGKLIQQGRQSKGLSQKDLATKICEKQQVVTDYEAGRGIPNNLILGKMERVLGIKLRGKERGQPIAPPGKK

Target ORF information:

RefSeq Version	NM_001259866.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259866.1

ORF Insert Sequence:

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbf1 ( NM_001275023.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261952.1 Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa21007
Clone ID Related Accession (Same CDS sequence)	NM_001275023.1
Accession Version	NM_001275023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multiprotein bridging factor 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGAAA TGATGCTGCC GCGTCCGCCG CCAGTGGTGC TCCACACACG 
GTCACCTCAG GACGCAGCGC CCGGCAACAG AACCATCAAC ACACGGATTC TGGAGGCTGG 
AGCACTGTAA GCGGCAGACG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261952.1
CDS	109..675
Translation	MSDWDSVTVLRKKAPKSSTLKTESAVNQARRQGVAVDTQQKYGAGTNKQHVTTKNTAKLDRETEELRHDKIPLDVGKLIQQGRQSKGLSQKDLATKICEKQQVVTDYEAGRGIPNNLILGKMERVLGIKLRGKERGQPIAPPGKK*NDAAASAASGAPHTVTSGRSARQQNHQHTDSGGWSTVSGRRK

Target ORF information:

RefSeq Version	NM_001275023.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multiprotein bridging factor 1 (mbf1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275023.1

ORF Insert Sequence:

ATGTCGGACT GGGACTCTGT GACTGTTCTT CGGAAGAAAG CACCGAAATC GTCGACCCTC 
AAGACGGAAT CGGCGGTCAA CCAGGCACGC CGTCAAGGAG TCGCCGTGGA TACCCAACAG 
AAGTATGGTG CTGGCACCAA CAAGCAGCAT GTGACCACCA AGAACACTGC CAAACTAGAC 
CGCGAGACCG AGGAGCTGCG CCACGACAAG ATTCCCCTCG ATGTGGGCAA GCTCATCCAG 
CAGGGACGAC AGAGCAAGGG CCTCAGCCAA AAGGACCTGG CAACTAAAAT CTGCGAAAAG 
CAGCAAGTGG TAACCGACTA CGAGGCCGGT CGCGGTATTC CAAACAACTT GATTCTGGGT 
AAAATGGAAC GCGTTCTCGG CATTAAGCTG CGCGGCAAGG AGCGCGGCCA ACCAATAGCA 
CCTCCCGGTA AAAAGTGAAA TGATGCTGCC GCGTCCGCCG CCAGTGGTGC TCCACACACG 
GTCACCTCAG GACGCAGCGC CCGGCAACAG AACCATCAAC ACACGGATTC TGGAGGCTGG 
AGCACTGTAA GCGGCAGACG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mbf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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