ssp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ssp2
Entrez Gene ID	39540
Full Name	short spindle 2
Synonyms	CG9028,Dmel\CG9028,Ssp2,sentin
General protein information	Preferred Names short spindle 2 Names CG9028 gene product from transcript CG9028-RC CG9028-PA CG9028-PC sentin short spindle ssp2-PA ssp2-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70C7-70C9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300136.1	NP_001287065.1	short spindle 2, isoform C
NM_140415.4	NP_648672.1	short spindle 2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes; the microtubule catastrophe-promoting complex Sentin-EB1 is a major factor responsible for this delay
Title: The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes.

In Drosophila melanogaster S2 cells, Dynamic microtubules (MT) tip-localizing protein Sentin, has been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs.
Title: Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.

The following ssp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18454	NM_140415.4 Latest version!	Drosophila melanogaster short spindle 2 (ssp2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18454	NM_001300136.1 Latest version!	Drosophila melanogaster short spindle 2 (ssp2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ssp2 ( NM_001300136.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287065.1 Drosophila melanogaster short spindle 2 (ssp2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18454
Clone ID Related Accession (Same CDS sequence)	NM_001300136.1 , NM_140415.4
Accession Version	NM_001300136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	short spindle 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATCC TGGACATCGA TGAGGCACTA AAGGATGATT CATATATATT TCTGGCGGAT 
AAGACCCACG ACGATGTCTC CAACATATTG CAGCAGTGGA AGACGAACAA TCAGCCGCCG 
TTGCAGCAAT TGCACTTGCA GTACAACAAC GAGGCCTTCA AAAACAATTC GAAAACCTTT 
ACTCGCCCAA AGCGTCGGAA TCTGGATTTC AGCCAAAATG GAGCGGGAAA TGACAGCGGT 
GTTGGAACCG GGTCGGTCGC GGGTGCCCCA GCAATGCTTC AGCTGGAAAT TGGCGGTCTT 
GTCACGACGA ATATGCACAA ATCCTTCCTG CAAGACACCT CACCGCCGTC TTCCATCTGC 
TCCTCCATGA ACACTGAACG GATGAACAAC TCGCTAATCA CCTCAGCAGA CTTTACGAAC 
TTGAACTTCC TTCAATCCAC AATTGGTCTT GAGCAGGATC CAAACCTTAC CACTACGCTT 
ACCAACCAGA CAACTGATAA CATGGGAATC GGCGGCAGCA TAGGCGGATA TACGCTCAGT 
GACATGATCC GGGATCGCAA GGCGCTGGAG TCGCTGACCA TGTGTGAGGA CGATGGGACT 
CTTATCAAAG ACTCGCACAT CGGGCAAATT GACGAAATAT CGTTGACACT GAGCAAAACT 
GCTTCCGGCT GCAGCACAAT GGACAACAGC ACAGACAGTA TGTCCATTCC CCTGGCCGCA 
GATCGAACGA TGCCCGTTGT GGTATCGGCA GTTTGTCCTG CCCTACAGCG TACCATGATT 
TTAGGGGAGG AGATGATCGG CGACACAACT TTTAACCTCG TGGATAGCCT TACAACTTCT 
GCGCTGCAAT CAGAGTCTGA GTCTCTGCCA GTCGATGGAA ATGCGACATT CAAAAGACCC 
ACTGCGAGTG CTACTACAGC GGACGAAACC CAGGTGCTAA CAGGTAGGCA GATGAATACT 
ACCTTCACCG ACGGTTGCAA CACTCCTGAG GGCCGCTGTG AAACGCCTGA AAATATTGAC 
AGGAAGCTGG CCTTGCTCAC CATGGAATCC TCCACACCAC TTACAACAAA CATGCGCTCA 
CACTGCTACC ACAATAACAA CAATAATAAC AAGGCAGGAT ATACCCCTAC CCTAAAGGGA 
AGGGGAGATA TGAACCTGTC CCCAATTGTT GGCGCAACAC CGCAAAAACC AACTGGAACC 
GCACCAGGGC GATTAAACAA CACCTTTGAG CCCGTGGCAA AAACAGCTCC GTTCAACGGC 
GAGAAGTTCG TGCTGGACAC TATGGAGCTC TTGGAGCAGA TCGAACAGCC GCTGGATGGC 
ACTTACAACC TGCAAATGTC TGAACAGCAC AGGCAGATGC AATGCGTCAT GGACTTGGCT 
GAGGCGGAAG TGGAGATGCT GGCCCAGCAG GGCGACGAAG AGCAATTCGA GAATATGCTT 
GCCGAGCTGG GCAAGGTGAA TACCCTAAAC GAGGAGCAAC TGAAGATGCA AAAGTCGCTA 
GATACCATCA AACGGCGCTT CCATAGAGAT GAGGCGGAGG CAGAGGAGCG TGAAGAGGAA 
TTGCAGCAGT CGGCAGCCGA AAAGGTGGAC ACCCCAGTTC TAAATCAGAG CCACTCCAGC 
AGCAACAGCA GCGGCGGTGG TGGCGAACGG CTGCTCAGTC GCCGAAGTCG TCTCTACGAC 
GATGTCAACC TTAGTGCAAT GCACGGCAGT AATGGAAGTT CGACCAGCAC CAACTCTGCC 
TCATTCATCG TCCAGCGTAG AGATATGCCA CAAGTGGAGC AGCCTGCACC AGATCCTGAT 
CCCGAACCAG CGGAAGAGCC TCCAAAGTCC CAGGTGACAG CAGAGATTGA TCCGGCAAGC 
TATAAGCTGC CGGAGCGAAG AGAGCGGGAT CGCGATCGCT TCAAGACCAT TAAGATTTCG 
AAAGAAATGC GACTGCAGCC GGAAATTATT GTGCCTTGTA TAGACGATGA ACCACAGCAA 
CTCGAGGACG AGCAAAAGCA ATTAGTAGAA GATGTTCGGT ACGAGGAGGA TCAGCGACAG 
GGTTCACCAC CGGGTCGACT TTCTCGTCGA GATCAGACCA CAGCAATTAG CAACAACGCA 
GATGCTAGTG CGAGTAACTA TCTGACCTAC AAAAAACCCA AGGAAAAGAG CCTTATCCAA 
CGACGTCCGC AGCAGCAGGA AGCGCCTTTA TTAGCCGAGC CAGCGCCTGC GTCGACTCTT 
CCCCAGCCGC GTTCCTTGTC CAGGCCTCGC TACATCAGTG GTCTTCAGAA GTTTACTACG 
GTGAGCAAGG CAACTTCAGC CGGAGCGGGA CTAAACGCCA CTCCAGCCGC TGCTATGCCG 
ACTACTGTTA CAGTGCCGGC CAACGGTAAC GGGGAGCTGA AGAGCCCTAT GGGTATTAAA 
TCCAAATCTT TCCACAACTT GTCCTCCACA ATGAGCGGCG TTGGCTCTGG AGCCCTGCCA 
AGACCTAGTT TGGGCGGTGG ACTGCGGCGT CCCAGTGCGC AGCCGAGCAA GCTTATGAGC 
GGATTGCGTG GACCAACTCA AGCCCAAGAG GATGACTCCG CTGTATTTAA AGTACCGAAA 
TTGGTGAGTG GGCTGCGTGC TCCTGGCCTT GCCCCGAAAC GAGCAGGAGC TAATGGTTTA 
GCTCGACCTT CGTCCGGATA TTACAGTCTG AGTGTTAAGA CGGCACCAGA GGCAGACACT 
CCTGAGAGCC TCTCCTCAGC CTCTTCGCGT GGCAGCCTCT ATGGCCACAA GGACTCCGGT 
AGAACCGTTA ACGGCGGAAA TGTTCCTCAA CAGAATCCAC AGCAAACCTT CGACATGCAG 
GCGCTTACCT CTAAGCTGAC CCAGGTGACT ACGGGAGCGA CCGGCATACC AAAGCCCTCG 
GGGTTGAGAC CACCATCGCA AATAAAGCGT AGCGGTCTGC CGCGACCTTC CAGCATTGTT 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287065.1
CDS	144..3092
Translation	MDILDIDEALKDDSYIFLADKTHDDVSNILQQWKTNNQPPLQQLHLQYNNEAFKNNSKTFTRPKRRNLDFSQNGAGNDSGVGTGSVAGAPAMLQLEIGGLVTTNMHKSFLQDTSPPSSICSSMNTERMNNSLITSADFTNLNFLQSTIGLEQDPNLTTTLTNQTTDNMGIGGSIGGYTLSDMIRDRKALESLTMCEDDGTLIKDSHIGQIDEISLTLSKTASGCSTMDNSTDSMSIPLAADRTMPVVVSAVCPALQRTMILGEEMIGDTTFNLVDSLTTSALQSESESLPVDGNATFKRPTASATTADETQVLTGRQMNTTFTDGCNTPEGRCETPENIDRKLALLTMESSTPLTTNMRSHCYHNNNNNNKAGYTPTLKGRGDMNLSPIVGATPQKPTGTAPGRLNNTFEPVAKTAPFNGEKFVLDTMELLEQIEQPLDGTYNLQMSEQHRQMQCVMDLAEAEVEMLAQQGDEEQFENMLAELGKVNTLNEEQLKMQKSLDTIKRRFHRDEAEAEEREEELQQSAAEKVDTPVLNQSHSSSNSSGGGGERLLSRRSRLYDDVNLSAMHGSNGSSTSTNSASFIVQRRDMPQVEQPAPDPDPEPAEEPPKSQVTAEIDPASYKLPERRERDRDRFKTIKISKEMRLQPEIIVPCIDDEPQQLEDEQKQLVEDVRYEEDQRQGSPPGRLSRRDQTTAISNNADASASNYLTYKKPKEKSLIQRRPQQQEAPLLAEPAPASTLPQPRSLSRPRYISGLQKFTTVSKATSAGAGLNATPAAAMPTTVTVPANGNGELKSPMGIKSKSFHNLSSTMSGVGSGALPRPSLGGGLRRPSAQPSKLMSGLRGPTQAQEDDSAVFKVPKLVSGLRAPGLAPKRAGANGLARPSSGYYSLSVKTAPEADTPESLSSASSRGSLYGHKDSGRTVNGGNVPQQNPQQTFDMQALTSKLTQVTTGATGIPKPSGLRPPSQIKRSGLPRPSSIVRR

Target ORF information:

RefSeq Version	NM_001300136.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster short spindle 2 (ssp2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300136.1

ORF Insert Sequence:

ATGGATATCC TGGACATCGA TGAGGCACTA AAGGATGATT CATATATATT TCTGGCGGAT 
AAGACCCACG ACGATGTCTC CAACATATTG CAGCAGTGGA AGACGAACAA TCAGCCGCCG 
TTGCAGCAAT TGCACTTGCA GTACAACAAC GAGGCCTTCA AAAACAATTC GAAAACCTTT 
ACTCGCCCAA AGCGTCGGAA TCTGGATTTC AGCCAAAATG GAGCGGGAAA TGACAGCGGT 
GTTGGAACCG GGTCGGTCGC GGGTGCCCCA GCAATGCTTC AGCTGGAAAT TGGCGGTCTT 
GTCACGACGA ATATGCACAA ATCCTTCCTG CAAGACACCT CACCGCCGTC TTCCATCTGC 
TCCTCCATGA ACACTGAACG GATGAACAAC TCGCTAATCA CCTCAGCAGA CTTTACGAAC 
TTGAACTTCC TTCAATCCAC AATTGGTCTT GAGCAGGATC CAAACCTTAC CACTACGCTT 
ACCAACCAGA CAACTGATAA CATGGGAATC GGCGGCAGCA TAGGCGGATA TACGCTCAGT 
GACATGATCC GGGATCGCAA GGCGCTGGAG TCGCTGACCA TGTGTGAGGA CGATGGGACT 
CTTATCAAAG ACTCGCACAT CGGGCAAATT GACGAAATAT CGTTGACACT GAGCAAAACT 
GCTTCCGGCT GCAGCACAAT GGACAACAGC ACAGACAGTA TGTCCATTCC CCTGGCCGCA 
GATCGAACGA TGCCCGTTGT GGTATCGGCA GTTTGTCCTG CCCTACAGCG TACCATGATT 
TTAGGGGAGG AGATGATCGG CGACACAACT TTTAACCTCG TGGATAGCCT TACAACTTCT 
GCGCTGCAAT CAGAGTCTGA GTCTCTGCCA GTCGATGGAA ATGCGACATT CAAAAGACCC 
ACTGCGAGTG CTACTACAGC GGACGAAACC CAGGTGCTAA CAGGTAGGCA GATGAATACT 
ACCTTCACCG ACGGTTGCAA CACTCCTGAG GGCCGCTGTG AAACGCCTGA AAATATTGAC 
AGGAAGCTGG CCTTGCTCAC CATGGAATCC TCCACACCAC TTACAACAAA CATGCGCTCA 
CACTGCTACC ACAATAACAA CAATAATAAC AAGGCAGGAT ATACCCCTAC CCTAAAGGGA 
AGGGGAGATA TGAACCTGTC CCCAATTGTT GGCGCAACAC CGCAAAAACC AACTGGAACC 
GCACCAGGGC GATTAAACAA CACCTTTGAG CCCGTGGCAA AAACAGCTCC GTTCAACGGC 
GAGAAGTTCG TGCTGGACAC TATGGAGCTC TTGGAGCAGA TCGAACAGCC GCTGGATGGC 
ACTTACAACC TGCAAATGTC TGAACAGCAC AGGCAGATGC AATGCGTCAT GGACTTGGCT 
GAGGCGGAAG TGGAGATGCT GGCCCAGCAG GGCGACGAAG AGCAATTCGA GAATATGCTT 
GCCGAGCTGG GCAAGGTGAA TACCCTAAAC GAGGAGCAAC TGAAGATGCA AAAGTCGCTA 
GATACCATCA AACGGCGCTT CCATAGAGAT GAGGCGGAGG CAGAGGAGCG TGAAGAGGAA 
TTGCAGCAGT CGGCAGCCGA AAAGGTGGAC ACCCCAGTTC TAAATCAGAG CCACTCCAGC 
AGCAACAGCA GCGGCGGTGG TGGCGAACGG CTGCTCAGTC GCCGAAGTCG TCTCTACGAC 
GATGTCAACC TTAGTGCAAT GCACGGCAGT AATGGAAGTT CGACCAGCAC CAACTCTGCC 
TCATTCATCG TCCAGCGTAG AGATATGCCA CAAGTGGAGC AGCCTGCACC AGATCCTGAT 
CCCGAACCAG CGGAAGAGCC TCCAAAGTCC CAGGTGACAG CAGAGATTGA TCCGGCAAGC 
TATAAGCTGC CGGAGCGAAG AGAGCGGGAT CGCGATCGCT TCAAGACCAT TAAGATTTCG 
AAAGAAATGC GACTGCAGCC GGAAATTATT GTGCCTTGTA TAGACGATGA ACCACAGCAA 
CTCGAGGACG AGCAAAAGCA ATTAGTAGAA GATGTTCGGT ACGAGGAGGA TCAGCGACAG 
GGTTCACCAC CGGGTCGACT TTCTCGTCGA GATCAGACCA CAGCAATTAG CAACAACGCA 
GATGCTAGTG CGAGTAACTA TCTGACCTAC AAAAAACCCA AGGAAAAGAG CCTTATCCAA 
CGACGTCCGC AGCAGCAGGA AGCGCCTTTA TTAGCCGAGC CAGCGCCTGC GTCGACTCTT 
CCCCAGCCGC GTTCCTTGTC CAGGCCTCGC TACATCAGTG GTCTTCAGAA GTTTACTACG 
GTGAGCAAGG CAACTTCAGC CGGAGCGGGA CTAAACGCCA CTCCAGCCGC TGCTATGCCG 
ACTACTGTTA CAGTGCCGGC CAACGGTAAC GGGGAGCTGA AGAGCCCTAT GGGTATTAAA 
TCCAAATCTT TCCACAACTT GTCCTCCACA ATGAGCGGCG TTGGCTCTGG AGCCCTGCCA 
AGACCTAGTT TGGGCGGTGG ACTGCGGCGT CCCAGTGCGC AGCCGAGCAA GCTTATGAGC 
GGATTGCGTG GACCAACTCA AGCCCAAGAG GATGACTCCG CTGTATTTAA AGTACCGAAA 
TTGGTGAGTG GGCTGCGTGC TCCTGGCCTT GCCCCGAAAC GAGCAGGAGC TAATGGTTTA 
GCTCGACCTT CGTCCGGATA TTACAGTCTG AGTGTTAAGA CGGCACCAGA GGCAGACACT 
CCTGAGAGCC TCTCCTCAGC CTCTTCGCGT GGCAGCCTCT ATGGCCACAA GGACTCCGGT 
AGAACCGTTA ACGGCGGAAA TGTTCCTCAA CAGAATCCAC AGCAAACCTT CGACATGCAG 
GCGCTTACCT CTAAGCTGAC CCAGGTGACT ACGGGAGCGA CCGGCATACC AAAGCCCTCG 
GGGTTGAGAC CACCATCGCA AATAAAGCGT AGCGGTCTGC CGCGACCTTC CAGCATTGTT 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssp2 ( NM_140415.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648672.1 Drosophila melanogaster short spindle 2 (ssp2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18454
Clone ID Related Accession (Same CDS sequence)	NM_001300136.1 , NM_140415.4
Accession Version	NM_140415.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	short spindle 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140415.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATCC TGGACATCGA TGAGGCACTA AAGGATGATT CATATATATT TCTGGCGGAT 
AAGACCCACG ACGATGTCTC CAACATATTG CAGCAGTGGA AGACGAACAA TCAGCCGCCG 
TTGCAGCAAT TGCACTTGCA GTACAACAAC GAGGCCTTCA AAAACAATTC GAAAACCTTT 
ACTCGCCCAA AGCGTCGGAA TCTGGATTTC AGCCAAAATG GAGCGGGAAA TGACAGCGGT 
GTTGGAACCG GGTCGGTCGC GGGTGCCCCA GCAATGCTTC AGCTGGAAAT TGGCGGTCTT 
GTCACGACGA ATATGCACAA ATCCTTCCTG CAAGACACCT CACCGCCGTC TTCCATCTGC 
TCCTCCATGA ACACTGAACG GATGAACAAC TCGCTAATCA CCTCAGCAGA CTTTACGAAC 
TTGAACTTCC TTCAATCCAC AATTGGTCTT GAGCAGGATC CAAACCTTAC CACTACGCTT 
ACCAACCAGA CAACTGATAA CATGGGAATC GGCGGCAGCA TAGGCGGATA TACGCTCAGT 
GACATGATCC GGGATCGCAA GGCGCTGGAG TCGCTGACCA TGTGTGAGGA CGATGGGACT 
CTTATCAAAG ACTCGCACAT CGGGCAAATT GACGAAATAT CGTTGACACT GAGCAAAACT 
GCTTCCGGCT GCAGCACAAT GGACAACAGC ACAGACAGTA TGTCCATTCC CCTGGCCGCA 
GATCGAACGA TGCCCGTTGT GGTATCGGCA GTTTGTCCTG CCCTACAGCG TACCATGATT 
TTAGGGGAGG AGATGATCGG CGACACAACT TTTAACCTCG TGGATAGCCT TACAACTTCT 
GCGCTGCAAT CAGAGTCTGA GTCTCTGCCA GTCGATGGAA ATGCGACATT CAAAAGACCC 
ACTGCGAGTG CTACTACAGC GGACGAAACC CAGGTGCTAA CAGGTAGGCA GATGAATACT 
ACCTTCACCG ACGGTTGCAA CACTCCTGAG GGCCGCTGTG AAACGCCTGA AAATATTGAC 
AGGAAGCTGG CCTTGCTCAC CATGGAATCC TCCACACCAC TTACAACAAA CATGCGCTCA 
CACTGCTACC ACAATAACAA CAATAATAAC AAGGCAGGAT ATACCCCTAC CCTAAAGGGA 
AGGGGAGATA TGAACCTGTC CCCAATTGTT GGCGCAACAC CGCAAAAACC AACTGGAACC 
GCACCAGGGC GATTAAACAA CACCTTTGAG CCCGTGGCAA AAACAGCTCC GTTCAACGGC 
GAGAAGTTCG TGCTGGACAC TATGGAGCTC TTGGAGCAGA TCGAACAGCC GCTGGATGGC 
ACTTACAACC TGCAAATGTC TGAACAGCAC AGGCAGATGC AATGCGTCAT GGACTTGGCT 
GAGGCGGAAG TGGAGATGCT GGCCCAGCAG GGCGACGAAG AGCAATTCGA GAATATGCTT 
GCCGAGCTGG GCAAGGTGAA TACCCTAAAC GAGGAGCAAC TGAAGATGCA AAAGTCGCTA 
GATACCATCA AACGGCGCTT CCATAGAGAT GAGGCGGAGG CAGAGGAGCG TGAAGAGGAA 
TTGCAGCAGT CGGCAGCCGA AAAGGTGGAC ACCCCAGTTC TAAATCAGAG CCACTCCAGC 
AGCAACAGCA GCGGCGGTGG TGGCGAACGG CTGCTCAGTC GCCGAAGTCG TCTCTACGAC 
GATGTCAACC TTAGTGCAAT GCACGGCAGT AATGGAAGTT CGACCAGCAC CAACTCTGCC 
TCATTCATCG TCCAGCGTAG AGATATGCCA CAAGTGGAGC AGCCTGCACC AGATCCTGAT 
CCCGAACCAG CGGAAGAGCC TCCAAAGTCC CAGGTGACAG CAGAGATTGA TCCGGCAAGC 
TATAAGCTGC CGGAGCGAAG AGAGCGGGAT CGCGATCGCT TCAAGACCAT TAAGATTTCG 
AAAGAAATGC GACTGCAGCC GGAAATTATT GTGCCTTGTA TAGACGATGA ACCACAGCAA 
CTCGAGGACG AGCAAAAGCA ATTAGTAGAA GATGTTCGGT ACGAGGAGGA TCAGCGACAG 
GGTTCACCAC CGGGTCGACT TTCTCGTCGA GATCAGACCA CAGCAATTAG CAACAACGCA 
GATGCTAGTG CGAGTAACTA TCTGACCTAC AAAAAACCCA AGGAAAAGAG CCTTATCCAA 
CGACGTCCGC AGCAGCAGGA AGCGCCTTTA TTAGCCGAGC CAGCGCCTGC GTCGACTCTT 
CCCCAGCCGC GTTCCTTGTC CAGGCCTCGC TACATCAGTG GTCTTCAGAA GTTTACTACG 
GTGAGCAAGG CAACTTCAGC CGGAGCGGGA CTAAACGCCA CTCCAGCCGC TGCTATGCCG 
ACTACTGTTA CAGTGCCGGC CAACGGTAAC GGGGAGCTGA AGAGCCCTAT GGGTATTAAA 
TCCAAATCTT TCCACAACTT GTCCTCCACA ATGAGCGGCG TTGGCTCTGG AGCCCTGCCA 
AGACCTAGTT TGGGCGGTGG ACTGCGGCGT CCCAGTGCGC AGCCGAGCAA GCTTATGAGC 
GGATTGCGTG GACCAACTCA AGCCCAAGAG GATGACTCCG CTGTATTTAA AGTACCGAAA 
TTGGTGAGTG GGCTGCGTGC TCCTGGCCTT GCCCCGAAAC GAGCAGGAGC TAATGGTTTA 
GCTCGACCTT CGTCCGGATA TTACAGTCTG AGTGTTAAGA CGGCACCAGA GGCAGACACT 
CCTGAGAGCC TCTCCTCAGC CTCTTCGCGT GGCAGCCTCT ATGGCCACAA GGACTCCGGT 
AGAACCGTTA ACGGCGGAAA TGTTCCTCAA CAGAATCCAC AGCAAACCTT CGACATGCAG 
GCGCTTACCT CTAAGCTGAC CCAGGTGACT ACGGGAGCGA CCGGCATACC AAAGCCCTCG 
GGGTTGAGAC CACCATCGCA AATAAAGCGT AGCGGTCTGC CGCGACCTTC CAGCATTGTT 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648672.1
CDS	144..3092
Translation	MDILDIDEALKDDSYIFLADKTHDDVSNILQQWKTNNQPPLQQLHLQYNNEAFKNNSKTFTRPKRRNLDFSQNGAGNDSGVGTGSVAGAPAMLQLEIGGLVTTNMHKSFLQDTSPPSSICSSMNTERMNNSLITSADFTNLNFLQSTIGLEQDPNLTTTLTNQTTDNMGIGGSIGGYTLSDMIRDRKALESLTMCEDDGTLIKDSHIGQIDEISLTLSKTASGCSTMDNSTDSMSIPLAADRTMPVVVSAVCPALQRTMILGEEMIGDTTFNLVDSLTTSALQSESESLPVDGNATFKRPTASATTADETQVLTGRQMNTTFTDGCNTPEGRCETPENIDRKLALLTMESSTPLTTNMRSHCYHNNNNNNKAGYTPTLKGRGDMNLSPIVGATPQKPTGTAPGRLNNTFEPVAKTAPFNGEKFVLDTMELLEQIEQPLDGTYNLQMSEQHRQMQCVMDLAEAEVEMLAQQGDEEQFENMLAELGKVNTLNEEQLKMQKSLDTIKRRFHRDEAEAEEREEELQQSAAEKVDTPVLNQSHSSSNSSGGGGERLLSRRSRLYDDVNLSAMHGSNGSSTSTNSASFIVQRRDMPQVEQPAPDPDPEPAEEPPKSQVTAEIDPASYKLPERRERDRDRFKTIKISKEMRLQPEIIVPCIDDEPQQLEDEQKQLVEDVRYEEDQRQGSPPGRLSRRDQTTAISNNADASASNYLTYKKPKEKSLIQRRPQQQEAPLLAEPAPASTLPQPRSLSRPRYISGLQKFTTVSKATSAGAGLNATPAAAMPTTVTVPANGNGELKSPMGIKSKSFHNLSSTMSGVGSGALPRPSLGGGLRRPSAQPSKLMSGLRGPTQAQEDDSAVFKVPKLVSGLRAPGLAPKRAGANGLARPSSGYYSLSVKTAPEADTPESLSSASSRGSLYGHKDSGRTVNGGNVPQQNPQQTFDMQALTSKLTQVTTGATGIPKPSGLRPPSQIKRSGLPRPSSIVRR

Target ORF information:

RefSeq Version	NM_140415.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster short spindle 2 (ssp2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140415.4

ORF Insert Sequence:

ATGGATATCC TGGACATCGA TGAGGCACTA AAGGATGATT CATATATATT TCTGGCGGAT 
AAGACCCACG ACGATGTCTC CAACATATTG CAGCAGTGGA AGACGAACAA TCAGCCGCCG 
TTGCAGCAAT TGCACTTGCA GTACAACAAC GAGGCCTTCA AAAACAATTC GAAAACCTTT 
ACTCGCCCAA AGCGTCGGAA TCTGGATTTC AGCCAAAATG GAGCGGGAAA TGACAGCGGT 
GTTGGAACCG GGTCGGTCGC GGGTGCCCCA GCAATGCTTC AGCTGGAAAT TGGCGGTCTT 
GTCACGACGA ATATGCACAA ATCCTTCCTG CAAGACACCT CACCGCCGTC TTCCATCTGC 
TCCTCCATGA ACACTGAACG GATGAACAAC TCGCTAATCA CCTCAGCAGA CTTTACGAAC 
TTGAACTTCC TTCAATCCAC AATTGGTCTT GAGCAGGATC CAAACCTTAC CACTACGCTT 
ACCAACCAGA CAACTGATAA CATGGGAATC GGCGGCAGCA TAGGCGGATA TACGCTCAGT 
GACATGATCC GGGATCGCAA GGCGCTGGAG TCGCTGACCA TGTGTGAGGA CGATGGGACT 
CTTATCAAAG ACTCGCACAT CGGGCAAATT GACGAAATAT CGTTGACACT GAGCAAAACT 
GCTTCCGGCT GCAGCACAAT GGACAACAGC ACAGACAGTA TGTCCATTCC CCTGGCCGCA 
GATCGAACGA TGCCCGTTGT GGTATCGGCA GTTTGTCCTG CCCTACAGCG TACCATGATT 
TTAGGGGAGG AGATGATCGG CGACACAACT TTTAACCTCG TGGATAGCCT TACAACTTCT 
GCGCTGCAAT CAGAGTCTGA GTCTCTGCCA GTCGATGGAA ATGCGACATT CAAAAGACCC 
ACTGCGAGTG CTACTACAGC GGACGAAACC CAGGTGCTAA CAGGTAGGCA GATGAATACT 
ACCTTCACCG ACGGTTGCAA CACTCCTGAG GGCCGCTGTG AAACGCCTGA AAATATTGAC 
AGGAAGCTGG CCTTGCTCAC CATGGAATCC TCCACACCAC TTACAACAAA CATGCGCTCA 
CACTGCTACC ACAATAACAA CAATAATAAC AAGGCAGGAT ATACCCCTAC CCTAAAGGGA 
AGGGGAGATA TGAACCTGTC CCCAATTGTT GGCGCAACAC CGCAAAAACC AACTGGAACC 
GCACCAGGGC GATTAAACAA CACCTTTGAG CCCGTGGCAA AAACAGCTCC GTTCAACGGC 
GAGAAGTTCG TGCTGGACAC TATGGAGCTC TTGGAGCAGA TCGAACAGCC GCTGGATGGC 
ACTTACAACC TGCAAATGTC TGAACAGCAC AGGCAGATGC AATGCGTCAT GGACTTGGCT 
GAGGCGGAAG TGGAGATGCT GGCCCAGCAG GGCGACGAAG AGCAATTCGA GAATATGCTT 
GCCGAGCTGG GCAAGGTGAA TACCCTAAAC GAGGAGCAAC TGAAGATGCA AAAGTCGCTA 
GATACCATCA AACGGCGCTT CCATAGAGAT GAGGCGGAGG CAGAGGAGCG TGAAGAGGAA 
TTGCAGCAGT CGGCAGCCGA AAAGGTGGAC ACCCCAGTTC TAAATCAGAG CCACTCCAGC 
AGCAACAGCA GCGGCGGTGG TGGCGAACGG CTGCTCAGTC GCCGAAGTCG TCTCTACGAC 
GATGTCAACC TTAGTGCAAT GCACGGCAGT AATGGAAGTT CGACCAGCAC CAACTCTGCC 
TCATTCATCG TCCAGCGTAG AGATATGCCA CAAGTGGAGC AGCCTGCACC AGATCCTGAT 
CCCGAACCAG CGGAAGAGCC TCCAAAGTCC CAGGTGACAG CAGAGATTGA TCCGGCAAGC 
TATAAGCTGC CGGAGCGAAG AGAGCGGGAT CGCGATCGCT TCAAGACCAT TAAGATTTCG 
AAAGAAATGC GACTGCAGCC GGAAATTATT GTGCCTTGTA TAGACGATGA ACCACAGCAA 
CTCGAGGACG AGCAAAAGCA ATTAGTAGAA GATGTTCGGT ACGAGGAGGA TCAGCGACAG 
GGTTCACCAC CGGGTCGACT TTCTCGTCGA GATCAGACCA CAGCAATTAG CAACAACGCA 
GATGCTAGTG CGAGTAACTA TCTGACCTAC AAAAAACCCA AGGAAAAGAG CCTTATCCAA 
CGACGTCCGC AGCAGCAGGA AGCGCCTTTA TTAGCCGAGC CAGCGCCTGC GTCGACTCTT 
CCCCAGCCGC GTTCCTTGTC CAGGCCTCGC TACATCAGTG GTCTTCAGAA GTTTACTACG 
GTGAGCAAGG CAACTTCAGC CGGAGCGGGA CTAAACGCCA CTCCAGCCGC TGCTATGCCG 
ACTACTGTTA CAGTGCCGGC CAACGGTAAC GGGGAGCTGA AGAGCCCTAT GGGTATTAAA 
TCCAAATCTT TCCACAACTT GTCCTCCACA ATGAGCGGCG TTGGCTCTGG AGCCCTGCCA 
AGACCTAGTT TGGGCGGTGG ACTGCGGCGT CCCAGTGCGC AGCCGAGCAA GCTTATGAGC 
GGATTGCGTG GACCAACTCA AGCCCAAGAG GATGACTCCG CTGTATTTAA AGTACCGAAA 
TTGGTGAGTG GGCTGCGTGC TCCTGGCCTT GCCCCGAAAC GAGCAGGAGC TAATGGTTTA 
GCTCGACCTT CGTCCGGATA TTACAGTCTG AGTGTTAAGA CGGCACCAGA GGCAGACACT 
CCTGAGAGCC TCTCCTCAGC CTCTTCGCGT GGCAGCCTCT ATGGCCACAA GGACTCCGGT 
AGAACCGTTA ACGGCGGAAA TGTTCCTCAA CAGAATCCAC AGCAAACCTT CGACATGCAG 
GCGCTTACCT CTAAGCTGAC CCAGGTGACT ACGGGAGCGA CCGGCATACC AAAGCCCTCG 
GGGTTGAGAC CACCATCGCA AATAAAGCGT AGCGGTCTGC CGCGACCTTC CAGCATTGTT 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ssp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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