Atg1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg1
Entrez Gene ID	39454
Full Name	Autophagy-related 1
Synonyms	ATG1,CG10967,DK-4,DmATG1,Dmel\CG10967,ULK1,UNC 51-like,Unc-51,Unc51,anon-WO0118547.287,atg1,dATG1,dAtg1,l(3)00305,unc-51,unc51
General protein information	Preferred Names Autophagy-related 1 Names CG10967 gene product from transcript CG10967-RA Atg1-PA Atg1-PB Autophagy-specific gene 1 CG10967-PA CG10967-PB autophagy-related gene 1 autophagy-specific gene1 pegarn uncoordinated movement-51
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69E2-69E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169962.2	NP_001163433.1	Autophagy-related 1, isoform B
NM_140344.3	NP_648601.1	Autophagy-related 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Atg1	NP_648601.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011295	XP_309350.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that autophagy-related 1 (Atg1) and ADUK/Ulk3 (CG8866) represent alternative catalytic components of a shared autophagy kinase complex.
Title: Atg1-independent induction of autophagy by the Drosophila Ulk3 homolog, ADUK.

dAtg1 has a previously unrecognized function for both regenerative and overgrowth-promoting apoptosis-induced proliferation (AiP) in eye and wing imaginal discs. dAtg1 acts genetically downstream of and is transcriptionally induced by JNK activity, and it is required for JNK-dependent production of mitogens such as Wingless for AiP.
Title: Autophagy-independent function of Atg1 for apoptosis-induced compensatory proliferation.

The Atg1-Tor pathway regulates yolk catabolism in Drosophila embryos.
Title: The Atg1-Tor pathway regulates yolk catabolism in Drosophila embryos.

Atg17 is required for the kinase activity of endogenous Atg1 in vivo
Title: Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila.

Unc-51 plays pivotal roles in the axonal and dendritic development of the Drosophila brain
Title: Unc-51/ATG1 controls axonal and dendritic development via kinesin-mediated vesicle transport in the Drosophila brain.

The following Atg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18376	NM_001169962.2 Latest version!	Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20869	NM_140344.3 Latest version!	Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg1 ( NM_001169962.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163433.1 Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18376
Clone ID Related Accession (Same CDS sequence)	NM_001169962.2
Accession Version	NM_001169962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169962.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG TCGGGGAATA TGAATACAGC TCCAAGGATA TGCTGGGCCA TGGCGCCTTT 
GCCGTCGTCT ACAAAGGACG TCATCGCAAG AAACACATGC CGGTGGCCAT CAAGTGCATC 
ACGAAGAAGG GACAACTGAA GACGCAGAAT CTGCTCGGCA AGGAGATCAA GATCCTGAAG 
GAGCTCACCG AGCTGCACCA TGAGAATGTG GTGGCTCTGC TGGACTGCAA GGAGTCCCAG 
GATTGCGTCA GCCTGGTCAT GGAGTATTGC AATGGCGGCG ACTTGGCGGA TTATCTGAGT 
GTCAAGGGGA CGCTCAGCGA GGATACCGTT AGACTCTTCC TCGTGCAACT AGCTGGTGCT 
ATGAAAGCAC TTTATACCAA AGGAATTGTG CATCGTGATC TCAAGCCACA AAACATTTTG 
CTATCGCACA ATTATGGCAA AACATTGCCA GCTCCATCGA AAATAACCCT GAAAATTGCG 
GATTTTGGGT TTGCGCGATT CCTGAACGAG GGCGCCATGG CAGCCACTCT GTGCGGCTCT 
CCCATGTATA TGGCGCCCGA GGTGATCATG TCGCTGCAGT ACGACTCCAA GGCGGATCTC 
TGGTCGCTGG GAACGATTGT CTACCAGTGT TTGACTGGCA AGGCGCCATT CTATGCACAA 
ACGCCCAACG AACTGAAGTC CTACTACGAG CAGAATGCTA ACCTGGCACC AAAAATTCCA 
AGTGGCGTGT CACCGGATCT GAGGGATCTG CTGCTGTGCC TGCTGCGCCG CAACTCCAAG 
GATCGCATCT CGTACGAGAG CTTCTTTGTT CACCGCTTCC TTCAGGGCAA GAAGGCGGCC 
GTGTCGCCAG GTGAGCCTAG TCAAGCAGCC AACCTGCGAC GACGAGTCTC TAAGAGTGGT 
GTTCTTCCAG TTGACATGCC ACCGCTTGGC GGAACACCAC CTGCCAAGGC CAAGAGCCCC 
TTGCAGCAGC AGCTGGAGCA GGAACTGAAG CTGGTCAAGC TGGCCGAGCA ACAGCAGAAG 
GAGCGCGAGG AGCAGGAGGC CCAGGAGGAC GAGAACACGG TGTCCGTGGT GGCCAATCCG 
GCCATTTGTG CCACCATCAC CAACGTAGGT GTCCTGTGCG ACAGTGAGAA CAACAGCGGT 
TCTTGCAGCT CCCACGAGGA CTCCGATGAC TTTGTGCTGG TGCCAAAGAA CCTTCCGGAG 
GACCAGCGAC AGGGTCTGGC CCAAGTCCAA GCCCAGCCCG CTTCCGGTGG CCAACGTCCA 
CAGCAGCAAC AAAATCAATC CAGTCCGCCA CGTCCCAGCA GTCTGCCCAT CTCTGAACCC 
AAACCAGTAC CGGCTCCCGC TCGGCGGCAG GTGGCCAGGC CCGGGCCGTT AACGGTAGCC 
ACACTGGGTG GCCAGCAGAT ACCCCGATCG CAGCCAATTA GCGTAAAGCA ACCGCGACCG 
GATCAGCGCA AGAGCAGTGT GAGCAGTGAC ATTAACTCGA TCTCACCGCC GGCGGTGCAG 
TTTGCCATTG GAACGCCGCC TACACGTATG CGTTCTGCCT CTGGTGGATC GCTGTCGGAG 
ACACCGCCGC CGCATGCACC GAGCACCTGG CAGGTGTCGC CAGGACACTC GCAATCGCCG 
TTGCGTCGAA GCGGCAATAG CTCGCCAGTT CTGCCATCCG CTGCGCTTAC CAAATTGCCC 
ACTTTGGGCA GTCCAACAAT GCTGGTGGCG CCGGGATCAT TGGGCTCGAT TGGTTCGGCG 
GGCAGTGGAT CGGAGAACAA CAATCAGCAT CACATGCTGG GACCGCGAGC TTTTACGCTG 
CCCGAACTGG GGGCCACTGG TGGCCTACAT AGTCTGCTGG ACACAGGAGC CGGCGGCGGT 
GGGGAACCAC ATGCATTCCA GGCCCCAGAA CTGTCCGAAG AGACGCTGAT GGATCGCGAG 
CACAACGAGA CATTGTCCAA GCTGAACTTT GTGCTGGCGC TCACCGATTG TATCCAGGAA 
GTTGCCGATT CGCGATGCGC ACCGCTCTCC ACGTTCATGG TGGCCGGCAG TCAGTCGGCG 
GCGCAAGCGG CGTCAGCGGA TGCACAGCAG ATACCGCCGC ACGCACCTGA GCACTGCAAA 
CGGGCCGAGC GCCTGGTGCT GCTCGTCCGG GGACTGCAGC TCCTATCGTC CGGGATGAAT 
CTGGCTTCCC AGCAGCTAAG CAATGGCCAG CTAAAGCCGT CGTCCAATGT GAAGAATGCT 
TTGCTCACCA TGAACGCCAA ATACCGGAGC ATGCTGTTCG AATCGAAACG CCTCAATGGC 
AGCGGACTAT TGCAGAAGGC GAATGCATTT AACATAACGG CGGACAAGAT TCTCTATGAC 
TACGCACTGG ATATGTGCCA GGCCGCTGCT CTGGATGAGC TACTGAAGAA CACGAAGAAT 
TGCTTCGAAC GTTACAACAC GGCACACATT CTGCTTCATT CGTTGGTGCA AAAGTGCAAT 
CATCCGCAGG ACAAAATGAT GCTGAACAAA TATCGCGATG CCGTTGAGAA GCGTTTGAGC 
ATATTGCAGC AGCATGGCTA TATATACATG ACCGATGAGA ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163433.1
CDS	673..3240
Translation	MNIVGEYEYSSKDMLGHGAFAVVYKGRHRKKHMPVAIKCITKKGQLKTQNLLGKEIKILKELTELHHENVVALLDCKESQDCVSLVMEYCNGGDLADYLSVKGTLSEDTVRLFLVQLAGAMKALYTKGIVHRDLKPQNILLSHNYGKTLPAPSKITLKIADFGFARFLNEGAMAATLCGSPMYMAPEVIMSLQYDSKADLWSLGTIVYQCLTGKAPFYAQTPNELKSYYEQNANLAPKIPSGVSPDLRDLLLCLLRRNSKDRISYESFFVHRFLQGKKAAVSPGEPSQAANLRRRVSKSGVLPVDMPPLGGTPPAKAKSPLQQQLEQELKLVKLAEQQQKEREEQEAQEDENTVSVVANPAICATITNVGVLCDSENNSGSCSSHEDSDDFVLVPKNLPEDQRQGLAQVQAQPASGGQRPQQQQNQSSPPRPSSLPISEPKPVPAPARRQVARPGPLTVATLGGQQIPRSQPISVKQPRPDQRKSSVSSDINSISPPAVQFAIGTPPTRMRSASGGSLSETPPPHAPSTWQVSPGHSQSPLRRSGNSSPVLPSAALTKLPTLGSPTMLVAPGSLGSIGSAGSGSENNNQHHMLGPRAFTLPELGATGGLHSLLDTGAGGGGEPHAFQAPELSEETLMDREHNETLSKLNFVLALTDCIQEVADSRCAPLSTFMVAGSQSAAQAASADAQQIPPHAPEHCKRAERLVLLVRGLQLLSSGMNLASQQLSNGQLKPSSNVKNALLTMNAKYRSMLFESKRLNGSGLLQKANAFNITADKILYDYALDMCQAAALDELLKNTKNCFERYNTAHILLHSLVQKCNHPQDKMMLNKYRDAVEKRLSILQQHGYIYMTDENA

Target ORF information:

RefSeq Version	NM_001169962.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169962.2

ORF Insert Sequence:

ATGAATATTG TCGGGGAATA TGAATACAGC TCCAAGGATA TGCTGGGCCA TGGCGCCTTT 
GCCGTCGTCT ACAAAGGACG TCATCGCAAG AAACACATGC CGGTGGCCAT CAAGTGCATC 
ACGAAGAAGG GACAACTGAA GACGCAGAAT CTGCTCGGCA AGGAGATCAA GATCCTGAAG 
GAGCTCACCG AGCTGCACCA TGAGAATGTG GTGGCTCTGC TGGACTGCAA GGAGTCCCAG 
GATTGCGTCA GCCTGGTCAT GGAGTATTGC AATGGCGGCG ACTTGGCGGA TTATCTGAGT 
GTCAAGGGGA CGCTCAGCGA GGATACCGTT AGACTCTTCC TCGTGCAACT AGCTGGTGCT 
ATGAAAGCAC TTTATACCAA AGGAATTGTG CATCGTGATC TCAAGCCACA AAACATTTTG 
CTATCGCACA ATTATGGCAA AACATTGCCA GCTCCATCGA AAATAACCCT GAAAATTGCG 
GATTTTGGGT TTGCGCGATT CCTGAACGAG GGCGCCATGG CAGCCACTCT GTGCGGCTCT 
CCCATGTATA TGGCGCCCGA GGTGATCATG TCGCTGCAGT ACGACTCCAA GGCGGATCTC 
TGGTCGCTGG GAACGATTGT CTACCAGTGT TTGACTGGCA AGGCGCCATT CTATGCACAA 
ACGCCCAACG AACTGAAGTC CTACTACGAG CAGAATGCTA ACCTGGCACC AAAAATTCCA 
AGTGGCGTGT CACCGGATCT GAGGGATCTG CTGCTGTGCC TGCTGCGCCG CAACTCCAAG 
GATCGCATCT CGTACGAGAG CTTCTTTGTT CACCGCTTCC TTCAGGGCAA GAAGGCGGCC 
GTGTCGCCAG GTGAGCCTAG TCAAGCAGCC AACCTGCGAC GACGAGTCTC TAAGAGTGGT 
GTTCTTCCAG TTGACATGCC ACCGCTTGGC GGAACACCAC CTGCCAAGGC CAAGAGCCCC 
TTGCAGCAGC AGCTGGAGCA GGAACTGAAG CTGGTCAAGC TGGCCGAGCA ACAGCAGAAG 
GAGCGCGAGG AGCAGGAGGC CCAGGAGGAC GAGAACACGG TGTCCGTGGT GGCCAATCCG 
GCCATTTGTG CCACCATCAC CAACGTAGGT GTCCTGTGCG ACAGTGAGAA CAACAGCGGT 
TCTTGCAGCT CCCACGAGGA CTCCGATGAC TTTGTGCTGG TGCCAAAGAA CCTTCCGGAG 
GACCAGCGAC AGGGTCTGGC CCAAGTCCAA GCCCAGCCCG CTTCCGGTGG CCAACGTCCA 
CAGCAGCAAC AAAATCAATC CAGTCCGCCA CGTCCCAGCA GTCTGCCCAT CTCTGAACCC 
AAACCAGTAC CGGCTCCCGC TCGGCGGCAG GTGGCCAGGC CCGGGCCGTT AACGGTAGCC 
ACACTGGGTG GCCAGCAGAT ACCCCGATCG CAGCCAATTA GCGTAAAGCA ACCGCGACCG 
GATCAGCGCA AGAGCAGTGT GAGCAGTGAC ATTAACTCGA TCTCACCGCC GGCGGTGCAG 
TTTGCCATTG GAACGCCGCC TACACGTATG CGTTCTGCCT CTGGTGGATC GCTGTCGGAG 
ACACCGCCGC CGCATGCACC GAGCACCTGG CAGGTGTCGC CAGGACACTC GCAATCGCCG 
TTGCGTCGAA GCGGCAATAG CTCGCCAGTT CTGCCATCCG CTGCGCTTAC CAAATTGCCC 
ACTTTGGGCA GTCCAACAAT GCTGGTGGCG CCGGGATCAT TGGGCTCGAT TGGTTCGGCG 
GGCAGTGGAT CGGAGAACAA CAATCAGCAT CACATGCTGG GACCGCGAGC TTTTACGCTG 
CCCGAACTGG GGGCCACTGG TGGCCTACAT AGTCTGCTGG ACACAGGAGC CGGCGGCGGT 
GGGGAACCAC ATGCATTCCA GGCCCCAGAA CTGTCCGAAG AGACGCTGAT GGATCGCGAG 
CACAACGAGA CATTGTCCAA GCTGAACTTT GTGCTGGCGC TCACCGATTG TATCCAGGAA 
GTTGCCGATT CGCGATGCGC ACCGCTCTCC ACGTTCATGG TGGCCGGCAG TCAGTCGGCG 
GCGCAAGCGG CGTCAGCGGA TGCACAGCAG ATACCGCCGC ACGCACCTGA GCACTGCAAA 
CGGGCCGAGC GCCTGGTGCT GCTCGTCCGG GGACTGCAGC TCCTATCGTC CGGGATGAAT 
CTGGCTTCCC AGCAGCTAAG CAATGGCCAG CTAAAGCCGT CGTCCAATGT GAAGAATGCT 
TTGCTCACCA TGAACGCCAA ATACCGGAGC ATGCTGTTCG AATCGAAACG CCTCAATGGC 
AGCGGACTAT TGCAGAAGGC GAATGCATTT AACATAACGG CGGACAAGAT TCTCTATGAC 
TACGCACTGG ATATGTGCCA GGCCGCTGCT CTGGATGAGC TACTGAAGAA CACGAAGAAT 
TGCTTCGAAC GTTACAACAC GGCACACATT CTGCTTCATT CGTTGGTGCA AAAGTGCAAT 
CATCCGCAGG ACAAAATGAT GCTGAACAAA TATCGCGATG CCGTTGAGAA GCGTTTGAGC 
ATATTGCAGC AGCATGGCTA TATATACATG ACCGATGAGA ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg1 ( NM_140344.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648601.1 Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20869
Clone ID Related Accession (Same CDS sequence)	NM_140344.3
Accession Version	NM_140344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140344.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG TCGGGGAATA TGAATACAGC TCCAAGGATA TGCTGGGCCA TGGCGCCTTT 
GCCGTCGTCT ACAAAGGACG TCATCGCAAG AAACACATGC CGGTGGCCAT CAAGTGCATC 
ACGAAGAAGG GACAACTGAA GACGCAGAAT CTGCTCGGCA AGGAGATCAA GATCCTGAAG 
GAGCTCACCG AGCTGCACCA TGAGAATGTG GTGGCTCTGC TGGACTGCAA GGAGTCCCAG 
GATTGCGTCA GCCTGGTCAT GGAGTATTGC AATGGCGGCG ACTTGGCGGA TTATCTGAGT 
GTCAAGGGGA CGCTCAGCGA GGATACCGTT AGACTCTTCC TCGTGCAACT AGCTGGTGCT 
ATGAAAGCAC TTTATACCAA AGGAATTGTG CATCGTGATC TCAAGCCACA AAACATTTTG 
CTATCGCACA ATTATGGCAA AACATTGCCA GCTCCATCGA AAATAACCCT GAAAATTGCG 
GATTTTGGGT TTGCGCGATT CCTGAACGAG GGCGCCATGG CAGCCACTCT GTGCGGCTCT 
CCCATGTATA TGGCGCCCGA GGTGATCATG TCGCTGCAGT ACGACTCCAA GGCGGATCTC 
TGGTCGCTGG GAACGATTGT CTACCAGTGT TTGACTGGCA AGGCGCCATT CTATGCACAA 
ACGCCCAACG AACTGAAGTC CTACTACGAG CAGAATGCTA ACCTGGCACC AAAAATTCCA 
AGTGGCGTGT CACCGGATCT GAGGGATCTG CTGCTGTGCC TGCTGCGCCG CAACTCCAAG 
GATCGCATCT CGTACGAGAG CTTCTTTGTT CACCGCTTCC TTCAGGGCAA GAAGGCGGCC 
GTGTCGCCAG TTGACATGCC ACCGCTTGGC GGAACACCAC CTGCCAAGGC CAAGAGCCCC 
TTGCAGCAGC AGCTGGAGCA GGAACTGAAG CTGGTCAAGC TGGCCGAGCA ACAGCAGAAG 
GAGCGCGAGG AGCAGGAGGC CCAGGAGGAC GAGAACACGG TGTCCGTGGT GGCCAATCCG 
GCCATTTGTG CCACCATCAC CAACGTAGGT GTCCTGTGCG ACAGTGAGAA CAACAGCGGT 
TCTTGCAGCT CCCACGAGGA CTCCGATGAC TTTGTGCTGG TGCCAAAGAA CCTTCCGGAG 
GACCAGCGAC AGGGTCTGGC CCAAGTCCAA GCCCAGCCCG CTTCCGGTGG CCAACGTCCA 
CAGCAGCAAC AAAATCAATC CAGTCCGCCA CGTCCCAGCA GTCTGCCCAT CTCTGAACCC 
AAACCAGTAC CGGCTCCCGC TCGGCGGCAG GTGGCCAGGC CCGGGCCGTT AACGGTAGCC 
ACACTGGGTG GCCAGCAGAT ACCCCGATCG CAGCCAATTA GCGTAAAGCA ACCGCGACCG 
GATCAGCGCA AGAGCAGTGT GAGCAGTGAC ATTAACTCGA TCTCACCGCC GGCGGTGCAG 
TTTGCCATTG GAACGCCGCC TACACGTATG CGTTCTGCCT CTGGTGGATC GCTGTCGGAG 
ACACCGCCGC CGCATGCACC GAGCACCTGG CAGGTGTCGC CAGGACACTC GCAATCGCCG 
TTGCGTCGAA GCGGCAATAG CTCGCCAGTT CTGCCATCCG CTGCGCTTAC CAAATTGCCC 
ACTTTGGGCA GTCCAACAAT GCTGGTGGCG CCGGGATCAT TGGGCTCGAT TGGTTCGGCG 
GGCAGTGGAT CGGAGAACAA CAATCAGCAT CACATGCTGG GACCGCGAGC TTTTACGCTG 
CCCGAACTGG GGGCCACTGG TGGCCTACAT AGTCTGCTGG ACACAGGAGC CGGCGGCGGT 
GGGGAACCAC ATGCATTCCA GGCCCCAGAA CTGTCCGAAG AGACGCTGAT GGATCGCGAG 
CACAACGAGA CATTGTCCAA GCTGAACTTT GTGCTGGCGC TCACCGATTG TATCCAGGAA 
GTTGCCGATT CGCGATGCGC ACCGCTCTCC ACGTTCATGG TGGCCGGCAG TCAGTCGGCG 
GCGCAAGCGG CGTCAGCGGA TGCACAGCAG ATACCGCCGC ACGCACCTGA GCACTGCAAA 
CGGGCCGAGC GCCTGGTGCT GCTCGTCCGG GGACTGCAGC TCCTATCGTC CGGGATGAAT 
CTGGCTTCCC AGCAGCTAAG CAATGGCCAG CTAAAGCCGT CGTCCAATGT GAAGAATGCT 
TTGCTCACCA TGAACGCCAA ATACCGGAGC ATGCTGTTCG AATCGAAACG CCTCAATGGC 
AGCGGACTAT TGCAGAAGGC GAATGCATTT AACATAACGG CGGACAAGAT TCTCTATGAC 
TACGCACTGG ATATGTGCCA GGCCGCTGCT CTGGATGAGC TACTGAAGAA CACGAAGAAT 
TGCTTCGAAC GTTACAACAC GGCACACATT CTGCTTCATT CGTTGGTGCA AAAGTGCAAT 
CATCCGCAGG ACAAAATGAT GCTGAACAAA TATCGCGATG CCGTTGAGAA GCGTTTGAGC 
ATATTGCAGC AGCATGGCTA TATATACATG ACCGATGAGA ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648601.1
CDS	673..3180
Translation	MNIVGEYEYSSKDMLGHGAFAVVYKGRHRKKHMPVAIKCITKKGQLKTQNLLGKEIKILKELTELHHENVVALLDCKESQDCVSLVMEYCNGGDLADYLSVKGTLSEDTVRLFLVQLAGAMKALYTKGIVHRDLKPQNILLSHNYGKTLPAPSKITLKIADFGFARFLNEGAMAATLCGSPMYMAPEVIMSLQYDSKADLWSLGTIVYQCLTGKAPFYAQTPNELKSYYEQNANLAPKIPSGVSPDLRDLLLCLLRRNSKDRISYESFFVHRFLQGKKAAVSPVDMPPLGGTPPAKAKSPLQQQLEQELKLVKLAEQQQKEREEQEAQEDENTVSVVANPAICATITNVGVLCDSENNSGSCSSHEDSDDFVLVPKNLPEDQRQGLAQVQAQPASGGQRPQQQQNQSSPPRPSSLPISEPKPVPAPARRQVARPGPLTVATLGGQQIPRSQPISVKQPRPDQRKSSVSSDINSISPPAVQFAIGTPPTRMRSASGGSLSETPPPHAPSTWQVSPGHSQSPLRRSGNSSPVLPSAALTKLPTLGSPTMLVAPGSLGSIGSAGSGSENNNQHHMLGPRAFTLPELGATGGLHSLLDTGAGGGGEPHAFQAPELSEETLMDREHNETLSKLNFVLALTDCIQEVADSRCAPLSTFMVAGSQSAAQAASADAQQIPPHAPEHCKRAERLVLLVRGLQLLSSGMNLASQQLSNGQLKPSSNVKNALLTMNAKYRSMLFESKRLNGSGLLQKANAFNITADKILYDYALDMCQAAALDELLKNTKNCFERYNTAHILLHSLVQKCNHPQDKMMLNKYRDAVEKRLSILQQHGYIYMTDENA

Target ORF information:

RefSeq Version	NM_140344.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 1 (Atg1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140344.3

ORF Insert Sequence:

ATGAATATTG TCGGGGAATA TGAATACAGC TCCAAGGATA TGCTGGGCCA TGGCGCCTTT 
GCCGTCGTCT ACAAAGGACG TCATCGCAAG AAACACATGC CGGTGGCCAT CAAGTGCATC 
ACGAAGAAGG GACAACTGAA GACGCAGAAT CTGCTCGGCA AGGAGATCAA GATCCTGAAG 
GAGCTCACCG AGCTGCACCA TGAGAATGTG GTGGCTCTGC TGGACTGCAA GGAGTCCCAG 
GATTGCGTCA GCCTGGTCAT GGAGTATTGC AATGGCGGCG ACTTGGCGGA TTATCTGAGT 
GTCAAGGGGA CGCTCAGCGA GGATACCGTT AGACTCTTCC TCGTGCAACT AGCTGGTGCT 
ATGAAAGCAC TTTATACCAA AGGAATTGTG CATCGTGATC TCAAGCCACA AAACATTTTG 
CTATCGCACA ATTATGGCAA AACATTGCCA GCTCCATCGA AAATAACCCT GAAAATTGCG 
GATTTTGGGT TTGCGCGATT CCTGAACGAG GGCGCCATGG CAGCCACTCT GTGCGGCTCT 
CCCATGTATA TGGCGCCCGA GGTGATCATG TCGCTGCAGT ACGACTCCAA GGCGGATCTC 
TGGTCGCTGG GAACGATTGT CTACCAGTGT TTGACTGGCA AGGCGCCATT CTATGCACAA 
ACGCCCAACG AACTGAAGTC CTACTACGAG CAGAATGCTA ACCTGGCACC AAAAATTCCA 
AGTGGCGTGT CACCGGATCT GAGGGATCTG CTGCTGTGCC TGCTGCGCCG CAACTCCAAG 
GATCGCATCT CGTACGAGAG CTTCTTTGTT CACCGCTTCC TTCAGGGCAA GAAGGCGGCC 
GTGTCGCCAG TTGACATGCC ACCGCTTGGC GGAACACCAC CTGCCAAGGC CAAGAGCCCC 
TTGCAGCAGC AGCTGGAGCA GGAACTGAAG CTGGTCAAGC TGGCCGAGCA ACAGCAGAAG 
GAGCGCGAGG AGCAGGAGGC CCAGGAGGAC GAGAACACGG TGTCCGTGGT GGCCAATCCG 
GCCATTTGTG CCACCATCAC CAACGTAGGT GTCCTGTGCG ACAGTGAGAA CAACAGCGGT 
TCTTGCAGCT CCCACGAGGA CTCCGATGAC TTTGTGCTGG TGCCAAAGAA CCTTCCGGAG 
GACCAGCGAC AGGGTCTGGC CCAAGTCCAA GCCCAGCCCG CTTCCGGTGG CCAACGTCCA 
CAGCAGCAAC AAAATCAATC CAGTCCGCCA CGTCCCAGCA GTCTGCCCAT CTCTGAACCC 
AAACCAGTAC CGGCTCCCGC TCGGCGGCAG GTGGCCAGGC CCGGGCCGTT AACGGTAGCC 
ACACTGGGTG GCCAGCAGAT ACCCCGATCG CAGCCAATTA GCGTAAAGCA ACCGCGACCG 
GATCAGCGCA AGAGCAGTGT GAGCAGTGAC ATTAACTCGA TCTCACCGCC GGCGGTGCAG 
TTTGCCATTG GAACGCCGCC TACACGTATG CGTTCTGCCT CTGGTGGATC GCTGTCGGAG 
ACACCGCCGC CGCATGCACC GAGCACCTGG CAGGTGTCGC CAGGACACTC GCAATCGCCG 
TTGCGTCGAA GCGGCAATAG CTCGCCAGTT CTGCCATCCG CTGCGCTTAC CAAATTGCCC 
ACTTTGGGCA GTCCAACAAT GCTGGTGGCG CCGGGATCAT TGGGCTCGAT TGGTTCGGCG 
GGCAGTGGAT CGGAGAACAA CAATCAGCAT CACATGCTGG GACCGCGAGC TTTTACGCTG 
CCCGAACTGG GGGCCACTGG TGGCCTACAT AGTCTGCTGG ACACAGGAGC CGGCGGCGGT 
GGGGAACCAC ATGCATTCCA GGCCCCAGAA CTGTCCGAAG AGACGCTGAT GGATCGCGAG 
CACAACGAGA CATTGTCCAA GCTGAACTTT GTGCTGGCGC TCACCGATTG TATCCAGGAA 
GTTGCCGATT CGCGATGCGC ACCGCTCTCC ACGTTCATGG TGGCCGGCAG TCAGTCGGCG 
GCGCAAGCGG CGTCAGCGGA TGCACAGCAG ATACCGCCGC ACGCACCTGA GCACTGCAAA 
CGGGCCGAGC GCCTGGTGCT GCTCGTCCGG GGACTGCAGC TCCTATCGTC CGGGATGAAT 
CTGGCTTCCC AGCAGCTAAG CAATGGCCAG CTAAAGCCGT CGTCCAATGT GAAGAATGCT 
TTGCTCACCA TGAACGCCAA ATACCGGAGC ATGCTGTTCG AATCGAAACG CCTCAATGGC 
AGCGGACTAT TGCAGAAGGC GAATGCATTT AACATAACGG CGGACAAGAT TCTCTATGAC 
TACGCACTGG ATATGTGCCA GGCCGCTGCT CTGGATGAGC TACTGAAGAA CACGAAGAAT 
TGCTTCGAAC GTTACAACAC GGCACACATT CTGCTTCATT CGTTGGTGCA AAAGTGCAAT 
CATCCGCAGG ACAAAATGAT GCTGAACAAA TATCGCGATG CCGTTGAGAA GCGTTTGAGC 
ATATTGCAGC AGCATGGCTA TATATACATG ACCGATGAGA ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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