simj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	simj
Entrez Gene ID	39212
Full Name	simjang
Synonyms	CG32067,CG7983,CG8000,Dmel\CG32067,SIMJ,anon-WO0118547.361,dp66,l(3)01814,l(3)j4A5,l(3)rN672,p66,p66/68-like
General protein information	Preferred Names simjang Names CG32067 gene product from transcript CG32067-RE CG32067-PA CG32067-PB CG32067-PC CG32067-PD CG32067-PE simj-PA simj-PB simj-PC simj-PD simj-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67E6-67E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168420.2	NP_729624.1	simjang, isoform A
NM_001274755.1	NP_001261684.1	simjang, isoform D
NM_168421.2	NP_729625.1	simjang, isoform B
NM_001274756.1	NP_001261685.1	simjang, isoform E
NM_140150.3	NP_648407.1	simjang, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neuronal knockdown of Drosophila GATAD2B orthologue, simj, resulted in impaired learning and altered synapse morphology
Title: GATAD2B loss-of-function mutations cause a recognisable syndrome with intellectual disability and are associated with learning deficits and synaptic undergrowth in Drosophila.

p66 is associated with the Mi-2/NURD complex and has developmental roles in Drosophila
Title: Developmental roles of the Mi-2/NURD-associated protein p66 in Drosophila.

The following simj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the simj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18170	NM_168421.2 Latest version!	Drosophila melanogaster simjang (simj), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa18170	NM_168420.2 Latest version!	Drosophila melanogaster simjang (simj), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa18171	NM_140150.3 Latest version!	Drosophila melanogaster simjang (simj), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa19892	NM_001274756.1 Latest version!	Drosophila melanogaster simjang (simj), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa19893	NM_001274755.1 Latest version!	Drosophila melanogaster simjang (simj), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

simj ( NM_168420.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729624.1 Drosophila melanogaster simjang (simj), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18170
Clone ID Related Accession (Same CDS sequence)	NM_168420.2 , NM_168421.2
Accession Version	NM_168420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	simjang, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168420.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGCCTT CAACAATAAC 
AACAAGAAGA GCAGCTCGTC CTCCGGATCG GCGAAGAATT CCAGCAGCTC GAACAGCATG 
GCGGAAGTTC AGCGGGCGGC GGAGCTGCAG CGCCAATATC TACTGGAGAT GATACCGCCC 
CAGCAGCAAA GTGGGGCCAG TGGCAGTCGG CAGAACAACT GGAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729624.1
CDS	673..3423
Translation	MAKMEVDDVVDLSLGSGRDPALTITPAPPPAALSNRDLRALTANNAGLTITPSASPATNNGTTSNSSNTNSNSSSTTTTTNNNNNSSSNTNSSSANNNNNNNSINASDGVTGATSGSIGGGQDENKVQRRVLRPRTEPKSYAEAPDIVLLPARTNGRQQNGNIDSETDDEEMPPYVPIKELSHAELKEREKSLRKLRQDLRNEETKLMLLKKLKQSQQVMKENLVVTPTSVSPNNPLSAIPAALTSKGSLSVTPTSAVPLPAHSKGSRSSLSGSGGPAVSISATNSRTGSSSSAAAAASAIAAAAMSGQHRSGSNSILPPPRSSLPAGATLAAGTTITPATSSSHRSSNLPPRTNLPNLTITPSVTITPTSAPPSSLKPRNVPGTISNSVSITPALPLSQSHQNQHNDQKNDRSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPPKPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNKKANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQRLASQSSPSPVDNHGSSGGGNSLSVSAVSSNSSSSHMQREREQLQASHASAAAALVNLPPSVTVIPTKCQTPTPATPRPTPPPPQSQATPPPSSGGSRSSRAAATAAAAAIAAQQAGILSPGPSPAHHHEPTTSSRSSRNDRLDHHHQSQQQQQRDQRERDQQQRDQQSQQQAQNYDKYSAATLAAAAHLSALGGMGGLNINSLASLGNLGNLGNLSQLGNLAALGGLGNFGGASTGSANPAAGLSGSSPAATAAAMQAFQQQLFRALGQGLGGNPQHMMQFAPLLYSYQMAMAQAAQVAAFNNNNKKSSSSSGSAKNSSSSNSMAEVQRAAELQRQYLLEMIPPQQQSGASGSRQNNWKA

Target ORF information:

RefSeq Version	NM_168420.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster simjang (simj), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168420.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGCCTT CAACAATAAC 
AACAAGAAGA GCAGCTCGTC CTCCGGATCG GCGAAGAATT CCAGCAGCTC GAACAGCATG 
GCGGAAGTTC AGCGGGCGGC GGAGCTGCAG CGCCAATATC TACTGGAGAT GATACCGCCC 
CAGCAGCAAA GTGGGGCCAG TGGCAGTCGG CAGAACAACT GGAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

simj ( NM_168421.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729625.1 Drosophila melanogaster simjang (simj), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18170
Clone ID Related Accession (Same CDS sequence)	NM_168420.2 , NM_168421.2
Accession Version	NM_168421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	simjang, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168421.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGCCTT CAACAATAAC 
AACAAGAAGA GCAGCTCGTC CTCCGGATCG GCGAAGAATT CCAGCAGCTC GAACAGCATG 
GCGGAAGTTC AGCGGGCGGC GGAGCTGCAG CGCCAATATC TACTGGAGAT GATACCGCCC 
CAGCAGCAAA GTGGGGCCAG TGGCAGTCGG CAGAACAACT GGAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729625.1
CDS	432..3182
Translation	MAKMEVDDVVDLSLGSGRDPALTITPAPPPAALSNRDLRALTANNAGLTITPSASPATNNGTTSNSSNTNSNSSSTTTTTNNNNNSSSNTNSSSANNNNNNNSINASDGVTGATSGSIGGGQDENKVQRRVLRPRTEPKSYAEAPDIVLLPARTNGRQQNGNIDSETDDEEMPPYVPIKELSHAELKEREKSLRKLRQDLRNEETKLMLLKKLKQSQQVMKENLVVTPTSVSPNNPLSAIPAALTSKGSLSVTPTSAVPLPAHSKGSRSSLSGSGGPAVSISATNSRTGSSSSAAAAASAIAAAAMSGQHRSGSNSILPPPRSSLPAGATLAAGTTITPATSSSHRSSNLPPRTNLPNLTITPSVTITPTSAPPSSLKPRNVPGTISNSVSITPALPLSQSHQNQHNDQKNDRSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPPKPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNKKANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQRLASQSSPSPVDNHGSSGGGNSLSVSAVSSNSSSSHMQREREQLQASHASAAAALVNLPPSVTVIPTKCQTPTPATPRPTPPPPQSQATPPPSSGGSRSSRAAATAAAAAIAAQQAGILSPGPSPAHHHEPTTSSRSSRNDRLDHHHQSQQQQQRDQRERDQQQRDQQSQQQAQNYDKYSAATLAAAAHLSALGGMGGLNINSLASLGNLGNLGNLSQLGNLAALGGLGNFGGASTGSANPAAGLSGSSPAATAAAMQAFQQQLFRALGQGLGGNPQHMMQFAPLLYSYQMAMAQAAQVAAFNNNNKKSSSSSGSAKNSSSSNSMAEVQRAAELQRQYLLEMIPPQQQSGASGSRQNNWKA

Target ORF information:

RefSeq Version	NM_168421.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster simjang (simj), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168421.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGCCTT CAACAATAAC 
AACAAGAAGA GCAGCTCGTC CTCCGGATCG GCGAAGAATT CCAGCAGCTC GAACAGCATG 
GCGGAAGTTC AGCGGGCGGC GGAGCTGCAG CGCCAATATC TACTGGAGAT GATACCGCCC 
CAGCAGCAAA GTGGGGCCAG TGGCAGTCGG CAGAACAACT GGAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

simj ( NM_140150.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648407.1 Drosophila melanogaster simjang (simj), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18171
Clone ID Related Accession (Same CDS sequence)	NM_140150.3
Accession Version	NM_140150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	simjang, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140150.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGGACTTG GTGGCAATCC GCAGCATATG ATGCAGTTCG CACCGCTTTT GTACTCTTAC 
CAAATGGCCA TGGCGCAGGC GGCACAAGTG GCAGCCTTCA ACAATAACAA CAAGAAGAGC 
AGCTCGTCCT CCGGATCGGC GAAGAATTCC AGCAGCTCGA ACAGCATGGC GGAAGTTCAG 
CGGGCGGCGG AGCTGCAGCG CCAATATCTA CTGGAGATGA TACCGCCCCA GCAGCAAAGT 
GGGGCCAGTG GCAGTCGGCA GAACAACTGG AAGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648407.1
CDS	673..3411
Translation	MAKMEVDDVVDLSLGSGRDPALTITPAPPPAALSNRDLRALTANNAGLTITPSASPATNNGTTSNSSNTNSNSSSTTTTTNNNNNSSSNTNSSSANNNNNNNSINASDGVTGATSGSIGGGQDENKVQRRVLRPRTEPKSYAEAPDIVLLPARTNGRQQNGNIDSETDDEEMPPYVPIKELSHAELKEREKSLRKLRQDLRNEETKLMLLKKLKQSQQVMKENLVVTPTSVSPNNPLSAIPAALTSKGSLSVTPTSAVPLPAHSKGSRSSLSGSGGPAVSISATNSRTGSSSSAAAAASAIAAAAMSGQHRSGSNSILPPPRSSLPAGATLAAGTTITPATSSSHRSSNLPPRTNLPNLTITPSVTITPTSAPPSSLKPRNVPGTISNSVSITPALPLSQSHQNQHNDQKNDRSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPPKPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNKKANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQRLASQSSPSPVDNHGSSGGGNSLSVSAVSSNSSSSHMQREREQLQASHASAAAALVNLPPSVTVIPTKCQTPTPATPRPTPPPPQSQATPPPSSGGSRSSRAAATAAAAAIAAQQAGILSPGPSPAHHHEPTTSSRSSRNDRLDHHHQSQQQQQRDQRERDQQQRDQQSQQQAQNYDKYSAATLAAAAHLSALGGMGGLNINSLASLGNLGNLGNLSQLGNLAALGGLGNFGGASTGSANPAAGLSGSSPAATAAAMQAFQQQLFRGLGGNPQHMMQFAPLLYSYQMAMAQAAQVAAFNNNNKKSSSSSGSAKNSSSSNSMAEVQRAAELQRQYLLEMIPPQQQSGASGSRQNNWKA

Target ORF information:

RefSeq Version	NM_140150.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster simjang (simj), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140150.3

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGGACTTG GTGGCAATCC GCAGCATATG ATGCAGTTCG CACCGCTTTT GTACTCTTAC 
CAAATGGCCA TGGCGCAGGC GGCACAAGTG GCAGCCTTCA ACAATAACAA CAAGAAGAGC 
AGCTCGTCCT CCGGATCGGC GAAGAATTCC AGCAGCTCGA ACAGCATGGC GGAAGTTCAG 
CGGGCGGCGG AGCTGCAGCG CCAATATCTA CTGGAGATGA TACCGCCCCA GCAGCAAAGT 
GGGGCCAGTG GCAGTCGGCA GAACAACTGG AAGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

simj ( NM_001274756.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261685.1 Drosophila melanogaster simjang (simj), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa19892
Clone ID Related Accession (Same CDS sequence)	NM_001274756.1
Accession Version	NM_001274756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	simjang, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGGTGA GATGACAATG 
GCCTTAACTG GGAAATTCAT AACTGGCTAC CAGGAATGCG ACTCAGTCAC CTTCAACAAT 
AACAACAAGA AGAGCAGCTC GTCCTCCGGA TCGGCGAAGA ATTCCAGCAG CTCGAACAGC 
ATGGCGGAAG TTCAGCGGGC GGCGGAGCTG CAGCGCCAAT ATCTACTGGA GATGATACCG 
CCCCAGCAGC AAAGTGGGGC CAGTGGCAGT CGGCAGAACA ACTGGAAGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261685.1
CDS	673..3486
Translation	MAKMEVDDVVDLSLGSGRDPALTITPAPPPAALSNRDLRALTANNAGLTITPSASPATNNGTTSNSSNTNSNSSSTTTTTNNNNNSSSNTNSSSANNNNNNNSINASDGVTGATSGSIGGGQDENKVQRRVLRPRTEPKSYAEAPDIVLLPARTNGRQQNGNIDSETDDEEMPPYVPIKELSHAELKEREKSLRKLRQDLRNEETKLMLLKKLKQSQQVMKENLVVTPTSVSPNNPLSAIPAALTSKGSLSVTPTSAVPLPAHSKGSRSSLSGSGGPAVSISATNSRTGSSSSAAAAASAIAAAAMSGQHRSGSNSILPPPRSSLPAGATLAAGTTITPATSSSHRSSNLPPRTNLPNLTITPSVTITPTSAPPSSLKPRNVPGTISNSVSITPALPLSQSHQNQHNDQKNDRSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPPKPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNKKANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQRLASQSSPSPVDNHGSSGGGNSLSVSAVSSNSSSSHMQREREQLQASHASAAAALVNLPPSVTVIPTKCQTPTPATPRPTPPPPQSQATPPPSSGGSRSSRAAATAAAAAIAAQQAGILSPGPSPAHHHEPTTSSRSSRNDRLDHHHQSQQQQQRDQRERDQQQRDQQSQQQAQNYDKYSAATLAAAAHLSALGGMGGLNINSLASLGNLGNLGNLSQLGNLAALGGLGNFGGASTGSANPAAGLSGSSPAATAAAMQAFQQQLFRALGQGLGGNPQHMMQFAPLLYSYQMAMAQAAQVAGEMTMALTGKFITGYQECDSVTFNNNNKKSSSSSGSAKNSSSSNSMAEVQRAAELQRQYLLEMIPPQQQSGASGSRQNNWKA

Target ORF information:

RefSeq Version	NM_001274756.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster simjang (simj), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274756.1

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATGTACCTG GGACCATAAG CAATTCTGTT TCCATCACGC CGGCGCTACC ACTGTCCCAA 
TCGCATCAGA ATCAGCACAA CGATCAGAAG AATGATCGCT CCACGCCAAG AGAAGATGCC 
CAGACACCAG CTCAAAGACA GGCGGCGGCT AAAATGGCGC TACGCAAGCA GCTGGAAAAG 
ACGCTGCTTC AGATTCCGCC ACCAAAGCCA CCACCGCCAG AGATGCACTT CATTCCCAAT 
CCCAGCAACA CGGATTTTGT GTATCTTCTT GGTTTGGAAA CCGTTGTGGA TTATCTGACC 
GTCAACAAGA AGGCCAATGC TGTGGCAGCA CCATTCCGTT GTGCCCAGTG CAAGATTGAT 
TTTACACCCG TGTGGAAGTG GGAGAAACAG ACCAACAAAG ATCCCAAAGT GATTTGCGAG 
CAGTGCGTCA CCTCGAATGT GAAGAAGGCA CTCAAGGCGG AGCACACCAA CCGACTGAAA 
CAGGCATTCG TTAAGGCGCT GCAGCAGGAG CAGGAGATCG AACAGCGTTT GGCCAGCCAG 
AGCAGTCCAT CGCCGGTGGA CAACCATGGA TCGAGTGGCG GCGGCAATTC GCTGTCTGTC 
TCCGCGGTGT CTAGCAATTC CTCATCGTCG CATATGCAGC GGGAACGGGA ACAGCTGCAG 
GCGTCGCATG CATCGGCGGC TGCTGCACTT GTCAATCTGC CGCCGTCGGT GACAGTGATA 
CCCACCAAAT GCCAGACGCC GACGCCAGCG ACACCGCGTC CAACGCCACC GCCACCACAA 
AGTCAAGCCA CACCACCGCC GTCATCTGGA GGATCGCGAT CCTCGCGAGC GGCGGCCACC 
GCCGCTGCAG CAGCTATTGC CGCTCAGCAG GCGGGTATCC TTAGTCCTGG GCCGTCGCCA 
GCGCATCACC ATGAGCCGAC TACATCCTCG AGATCCTCCC GCAACGATCG ACTCGATCAT 
CACCATCAGT CGCAGCAGCA ACAGCAGCGC GATCAGCGGG AAAGAGATCA GCAGCAGCGG 
GATCAGCAGT CGCAACAGCA GGCCCAAAAC TACGACAAAT ACTCGGCGGC CACCCTGGCG 
GCGGCTGCTC ATCTGAGTGC TCTTGGCGGC ATGGGCGGAT TGAATATCAA TAGCTTGGCC 
AGTCTAGGCA ACCTTGGCAA TTTGGGCAAC CTCAGCCAGC TGGGCAATCT GGCTGCGCTG 
GGAGGATTGG GTAATTTTGG CGGAGCATCG ACGGGTTCGG CTAATCCGGC GGCAGGACTG 
AGCGGTTCCT CGCCAGCTGC TACGGCGGCA GCCATGCAGG CGTTCCAGCA GCAGCTCTTT 
AGAGCTTTGG GACAAGGACT TGGTGGCAAT CCGCAGCATA TGATGCAGTT CGCACCGCTT 
TTGTACTCTT ACCAAATGGC CATGGCGCAG GCGGCACAAG TGGCAGGTGA GATGACAATG 
GCCTTAACTG GGAAATTCAT AACTGGCTAC CAGGAATGCG ACTCAGTCAC CTTCAACAAT 
AACAACAAGA AGAGCAGCTC GTCCTCCGGA TCGGCGAAGA ATTCCAGCAG CTCGAACAGC 
ATGGCGGAAG TTCAGCGGGC GGCGGAGCTG CAGCGCCAAT ATCTACTGGA GATGATACCG 
CCCCAGCAGC AAAGTGGGGC CAGTGGCAGT CGGCAGAACA ACTGGAAGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

simj ( NM_001274755.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261684.1 Drosophila melanogaster simjang (simj), transcript variant D, mRNA.

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CloneID	OFa19893
Clone ID Related Accession (Same CDS sequence)	NM_001274755.1
Accession Version	NM_001274755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	simjang, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATCCTAATA TTTCGGATAA TTCGAAGGTA CCTGGGACCA TAAGCAATTC TGTTTCCATC 
ACGCCGGCGC TACCACTGTC CCAATCGCAT CAGAATCAGC ACAACGATCA GAAGAATGAT 
CGCTCCACGC CAAGAGAAGA TGCCCAGACA CCAGCTCAAA GACAGGCGGC GGCTAAAATG 
GCGCTACGCA AGCAGCTGGA AAAGACGCTG CTTCAGATTC CGCCACCAAA GCCACCACCG 
CCAGAGATGC ACTTCATTCC CAATCCCAGC AACACGGATT TTGTGTATCT TCTTGGTTTG 
GAAACCGTTG TGGATTATCT GACCGTCAAC AAGAAGGCCA ATGCTGTGGC AGCACCATTC 
CGTTGTGCCC AGTGCAAGAT TGATTTTACA CCCGTGTGGA AGTGGGAGAA ACAGACCAAC 
AAAGATCCCA AAGTGATTTG CGAGCAGTGC GTCACCTCGA ATGTGAAGAA GGCACTCAAG 
GCGGAGCACA CCAACCGACT GAAACAGGCA TTCGTTAAGG CGCTGCAGCA GGAGCAGGAG 
ATCGAACAGC GTTTGGCCAG CCAGAGCAGT CCATCGCCGG TGGACAACCA TGGATCGAGT 
GGCGGCGGCA ATTCGCTGTC TGTCTCCGCG GTGTCTAGCA ATTCCTCATC GTCGCATATG 
CAGCGGGAAC GGGAACAGCT GCAGGCGTCG CATGCATCGG CGGCTGCTGC ACTTGTCAAT 
CTGCCGCCGT CGGTGACAGT GATACCCACC AAATGCCAGA CGCCGACGCC AGCGACACCG 
CGTCCAACGC CACCGCCACC ACAAAGTCAA GCCACACCAC CGCCGTCATC TGGAGGATCG 
CGATCCTCGC GAGCGGCGGC CACCGCCGCT GCAGCAGCTA TTGCCGCTCA GCAGGCGGGT 
ATCCTTAGTC CTGGGCCGTC GCCAGCGCAT CACCATGAGC CGACTACATC CTCGAGATCC 
TCCCGCAACG ATCGACTCGA TCATCACCAT CAGTCGCAGC AGCAACAGCA GCGCGATCAG 
CGGGAAAGAG ATCAGCAGCA GCGGGATCAG CAGTCGCAAC AGCAGGCCCA AAACTACGAC 
AAATACTCGG CGGCCACCCT GGCGGCGGCT GCTCATCTGA GTGCTCTTGG CGGCATGGGC 
GGATTGAATA TCAATAGCTT GGCCAGTCTA GGCAACCTTG GCAATTTGGG CAACCTCAGC 
CAGCTGGGCA ATCTGGCTGC GCTGGGAGGA TTGGGTAATT TTGGCGGAGC ATCGACGGGT 
TCGGCTAATC CGGCGGCAGG ACTGAGCGGT TCCTCGCCAG CTGCTACGGC GGCAGCCATG 
CAGGCGTTCC AGCAGCAGCT CTTTAGAGCT TTGGGACAAG GACTTGGTGG CAATCCGCAG 
CATATGATGC AGTTCGCACC GCTTTTGTAC TCTTACCAAA TGGCCATGGC GCAGGCGGCA 
CAAGTGGCAG CCTTCAACAA TAACAACAAG AAGAGCAGCT CGTCCTCCGG ATCGGCGAAG 
AATTCCAGCA GCTCGAACAG CATGGCGGAA GTTCAGCGGG CGGCGGAGCT GCAGCGCCAA 
TATCTACTGG AGATGATACC GCCCCAGCAG CAAAGTGGGG CCAGTGGCAG TCGGCAGAAC 
AACTGGAAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261684.1
CDS	673..3447
Translation	MAKMEVDDVVDLSLGSGRDPALTITPAPPPAALSNRDLRALTANNAGLTITPSASPATNNGTTSNSSNTNSNSSSTTTTTNNNNNSSSNTNSSSANNNNNNNSINASDGVTGATSGSIGGGQDENKVQRRVLRPRTEPKSYAEAPDIVLLPARTNGRQQNGNIDSETDDEEMPPYVPIKELSHAELKEREKSLRKLRQDLRNEETKLMLLKKLKQSQQVMKENLVVTPTSVSPNNPLSAIPAALTSKGSLSVTPTSAVPLPAHSKGSRSSLSGSGGPAVSISATNSRTGSSSSAAAAASAIAAAAMSGQHRSGSNSILPPPRSSLPAGATLAAGTTITPATSSSHRSSNLPPRTNLPNLTITPSVTITPTSAPPSSLKPRNPNISDNSKVPGTISNSVSITPALPLSQSHQNQHNDQKNDRSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPPKPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNKKANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQRLASQSSPSPVDNHGSSGGGNSLSVSAVSSNSSSSHMQREREQLQASHASAAAALVNLPPSVTVIPTKCQTPTPATPRPTPPPPQSQATPPPSSGGSRSSRAAATAAAAAIAAQQAGILSPGPSPAHHHEPTTSSRSSRNDRLDHHHQSQQQQQRDQRERDQQQRDQQSQQQAQNYDKYSAATLAAAAHLSALGGMGGLNINSLASLGNLGNLGNLSQLGNLAALGGLGNFGGASTGSANPAAGLSGSSPAATAAAMQAFQQQLFRALGQGLGGNPQHMMQFAPLLYSYQMAMAQAAQVAAFNNNNKKSSSSSGSAKNSSSSNSMAEVQRAAELQRQYLLEMIPPQQQSGASGSRQNNWKA

Target ORF information:

RefSeq Version	NM_001274755.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster simjang (simj), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274755.1

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTCGA CGATGTGGTC GACTTGAGTT TGGGGTCCGG TCGTGACCCG 
GCGTTGACCA TAACGCCGGC TCCGCCGCCG GCGGCGCTCA GTAATCGGGA TCTGCGTGCG 
CTGACAGCCA ACAATGCTGG CCTGACCATC ACTCCGTCAG CATCGCCGGC GACCAATAAT 
GGCACCACCT CCAATAGCAG TAACACAAAC TCCAACAGCA GCAGCACCAC CACCACCACC 
AACAACAACA ACAACAGTAG CAGTAACACC AATAGCTCCA GCGCAAACAA CAATAACAAC 
AACAACAGCA TTAACGCCAG TGATGGTGTC ACAGGTGCGA CAAGTGGATC CATAGGCGGC 
GGTCAGGATG AGAACAAGGT TCAGCGGAGA GTACTGCGAC CACGAACCGA ACCCAAATCC 
TATGCCGAGG CCCCAGACAT TGTGTTACTA CCCGCTCGCA CAAATGGCCG TCAGCAGAAT 
GGAAACATCG ATTCGGAGAC GGATGACGAG GAGATGCCCC CGTATGTGCC CATCAAGGAG 
CTGAGCCATG CAGAGCTCAA GGAGCGCGAG AAGAGTCTGA GGAAGCTGCG GCAGGATCTG 
CGCAATGAGG AGACCAAACT GATGCTGCTC AAGAAACTCA AGCAGTCGCA GCAGGTGATG 
AAGGAGAATC TGGTGGTGAC GCCGACCAGC GTATCGCCCA ACAATCCGCT ATCGGCGATA 
CCCGCCGCTC TCACCTCGAA GGGATCGCTG AGTGTGACGC CCACGTCGGC GGTACCCCTG 
CCCGCCCACA GCAAGGGCAG CAGGAGCAGT TTGAGCGGCA GCGGCGGACC GGCAGTGAGC 
ATAAGCGCCA CCAACAGTCG AACGGGCAGC TCGAGTTCAG CGGCGGCGGC TGCTTCGGCA 
ATTGCAGCGG CCGCCATGAG CGGCCAACAT CGCAGCGGTA GCAACTCCAT ACTGCCGCCG 
CCACGATCAT CGCTGCCCGC TGGTGCCACC TTGGCCGCCG GCACTACCAT CACGCCCGCC 
ACCTCCTCAT CGCACCGCTC GAGCAATTTG CCGCCGCGCA CCAACCTGCC GAATCTCACC 
ATCACGCCAT CGGTGACCAT CACGCCCACA TCGGCACCGC CCTCCAGTCT GAAACCACGC 
AATCCTAATA TTTCGGATAA TTCGAAGGTA CCTGGGACCA TAAGCAATTC TGTTTCCATC 
ACGCCGGCGC TACCACTGTC CCAATCGCAT CAGAATCAGC ACAACGATCA GAAGAATGAT 
CGCTCCACGC CAAGAGAAGA TGCCCAGACA CCAGCTCAAA GACAGGCGGC GGCTAAAATG 
GCGCTACGCA AGCAGCTGGA AAAGACGCTG CTTCAGATTC CGCCACCAAA GCCACCACCG 
CCAGAGATGC ACTTCATTCC CAATCCCAGC AACACGGATT TTGTGTATCT TCTTGGTTTG 
GAAACCGTTG TGGATTATCT GACCGTCAAC AAGAAGGCCA ATGCTGTGGC AGCACCATTC 
CGTTGTGCCC AGTGCAAGAT TGATTTTACA CCCGTGTGGA AGTGGGAGAA ACAGACCAAC 
AAAGATCCCA AAGTGATTTG CGAGCAGTGC GTCACCTCGA ATGTGAAGAA GGCACTCAAG 
GCGGAGCACA CCAACCGACT GAAACAGGCA TTCGTTAAGG CGCTGCAGCA GGAGCAGGAG 
ATCGAACAGC GTTTGGCCAG CCAGAGCAGT CCATCGCCGG TGGACAACCA TGGATCGAGT 
GGCGGCGGCA ATTCGCTGTC TGTCTCCGCG GTGTCTAGCA ATTCCTCATC GTCGCATATG 
CAGCGGGAAC GGGAACAGCT GCAGGCGTCG CATGCATCGG CGGCTGCTGC ACTTGTCAAT 
CTGCCGCCGT CGGTGACAGT GATACCCACC AAATGCCAGA CGCCGACGCC AGCGACACCG 
CGTCCAACGC CACCGCCACC ACAAAGTCAA GCCACACCAC CGCCGTCATC TGGAGGATCG 
CGATCCTCGC GAGCGGCGGC CACCGCCGCT GCAGCAGCTA TTGCCGCTCA GCAGGCGGGT 
ATCCTTAGTC CTGGGCCGTC GCCAGCGCAT CACCATGAGC CGACTACATC CTCGAGATCC 
TCCCGCAACG ATCGACTCGA TCATCACCAT CAGTCGCAGC AGCAACAGCA GCGCGATCAG 
CGGGAAAGAG ATCAGCAGCA GCGGGATCAG CAGTCGCAAC AGCAGGCCCA AAACTACGAC 
AAATACTCGG CGGCCACCCT GGCGGCGGCT GCTCATCTGA GTGCTCTTGG CGGCATGGGC 
GGATTGAATA TCAATAGCTT GGCCAGTCTA GGCAACCTTG GCAATTTGGG CAACCTCAGC 
CAGCTGGGCA ATCTGGCTGC GCTGGGAGGA TTGGGTAATT TTGGCGGAGC ATCGACGGGT 
TCGGCTAATC CGGCGGCAGG ACTGAGCGGT TCCTCGCCAG CTGCTACGGC GGCAGCCATG 
CAGGCGTTCC AGCAGCAGCT CTTTAGAGCT TTGGGACAAG GACTTGGTGG CAATCCGCAG 
CATATGATGC AGTTCGCACC GCTTTTGTAC TCTTACCAAA TGGCCATGGC GCAGGCGGCA 
CAAGTGGCAG CCTTCAACAA TAACAACAAG AAGAGCAGCT CGTCCTCCGG ATCGGCGAAG 
AATTCCAGCA GCTCGAACAG CATGGCGGAA GTTCAGCGGG CGGCGGAGCT GCAGCGCCAA 
TATCTACTGG AGATGATACC GCCCCAGCAG CAAAGTGGGG CCAGTGGCAG TCGGCAGAAC 
AACTGGAAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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simj cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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