CG42268 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG42268
Entrez Gene ID	39145
Full Name	CG42268 gene product from transcript CG42268-RI
Synonyms	CG14170,CG32044,CG32046,CG6740,Dmel\CG42268,Dmel_CG32044,Dmel_CG32046
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C6-67C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168368.2	NP_729544.2	uncharacterized protein, isoform D
NM_168367.1	NP_729543.1	uncharacterized protein, isoform F
NM_001274722.1	NP_001261651.1	uncharacterized protein, isoform L
NM_001274720.1	NP_001261649.1	uncharacterized protein, isoform J
NM_001274723.1	NP_001261652.1	uncharacterized protein, isoform M
NM_168366.4	NP_729542.4	uncharacterized protein, isoform H
NM_001274719.1	NP_001261648.1	uncharacterized protein, isoform I
NM_001274721.1	NP_001261650.1	uncharacterized protein, isoform K

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG42268 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG42268 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20807	NM_001274720.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant J (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20807	NM_001274721.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant K (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18097	NM_168366.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant H (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20822	NM_001274723.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant M (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa18096	NM_168368.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa18098	NM_168367.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant F (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20806	NM_001274722.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant L (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20820	NM_001274719.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant I (CG42268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG42268 ( NM_001274720.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261649.1 Drosophila melanogaster uncharacterized protein, transcript variant J (CG42268), mRNA.

Price & Availability↑

CloneID	OFa20807
Clone ID Related Accession (Same CDS sequence)	NM_001274720.1 , NM_001274721.1
Accession Version	NM_001274720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTATG ACAAGTCCTT CCGCAATGCG ATGATCCAGG ATGTGCTGCA GTTCAAGAAG 
CAGCTGCTCC GACTGCGGCG AATACTCCAG GAGACGGAAA CGCTCAATCC CTTCGAGAAC 
GATAACGTCC AGCTGTTCGC CGCCTGCGGA TTGGATAGCA AGCAATTGAA TGACATCGAT 
TTGGCCAGCC TAACCTCGTC CACGACGGAG GATCCGCTAC AGGAGCTAAC GGATCTACGT 
AGACAGGTGG TTTACCTTCA GGGTCAAGTG GACGATCGTG ATCGCACCAT TCGCCTGCAG 
CGCGATCTCA TCGAGCAACT GGAGGCCGAG AAGAGGCAGA AGGCTTCGCA TGGACCGGGA 
TCTGGCGCGG ATCCGGGCAA GGAGCTAATC AGCATGGCCA CCCAAACGGA GCGGACACGA 
CCACTTGCCA TTGGTGCGGA GGGTTTGTCC AGAAGTAAGC CCGAATATAC CAGTTATACC 
ACGCACTTTC CGATGCTCCA CTTGCACGAT TCCACGCTGG CAGCCACCAC GATAATCCGT 
CATCATCAGA GCAACCATGA GCAGCAACAG CCGGAGAAAT CCTGTCCAGC TCTCAGCCAA 
ACCCGCCGGC ACACCATTAT TTCCACCACG CTGACCAACT ACAATCAGCA GCTGGCGGCC 
GCATTTCCCG ATGCTACGCG ACGCTCCTCC ATCGGTTGGG ATACCACTCC GACCACGCCC 
ACCCCGCATG GCAACGCCTC CAAGCCGGTA AGGATTACGC TAATCGGCGA GGCACAGCCG 
CTGCAGAGCA AGTCATCAAC CGGATCGCTA TGCTCGTCAT CATCATCGTC GTCGTCATCC 
TCCTCATCAT CAACATCCTT GTTGGCACAA AATGCCAATG TGGTCAAGAT GCGATATACC 
AATGGCTGTC AGAGTGTTAA GAACGGATCG AGTGCCCATT ATCAGCCGTT GTACAACAGC 
AACAAGATGA CAAGCCCAAC CGTAACTATA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261649.1
CDS	907..1902
Translation	MLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQVVYLQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_001274720.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant J (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274720.1

ORF Insert Sequence:

ATGTTGTATG ACAAGTCCTT CCGCAATGCG ATGATCCAGG ATGTGCTGCA GTTCAAGAAG 
CAGCTGCTCC GACTGCGGCG AATACTCCAG GAGACGGAAA CGCTCAATCC CTTCGAGAAC 
GATAACGTCC AGCTGTTCGC CGCCTGCGGA TTGGATAGCA AGCAATTGAA TGACATCGAT 
TTGGCCAGCC TAACCTCGTC CACGACGGAG GATCCGCTAC AGGAGCTAAC GGATCTACGT 
AGACAGGTGG TTTACCTTCA GGGTCAAGTG GACGATCGTG ATCGCACCAT TCGCCTGCAG 
CGCGATCTCA TCGAGCAACT GGAGGCCGAG AAGAGGCAGA AGGCTTCGCA TGGACCGGGA 
TCTGGCGCGG ATCCGGGCAA GGAGCTAATC AGCATGGCCA CCCAAACGGA GCGGACACGA 
CCACTTGCCA TTGGTGCGGA GGGTTTGTCC AGAAGTAAGC CCGAATATAC CAGTTATACC 
ACGCACTTTC CGATGCTCCA CTTGCACGAT TCCACGCTGG CAGCCACCAC GATAATCCGT 
CATCATCAGA GCAACCATGA GCAGCAACAG CCGGAGAAAT CCTGTCCAGC TCTCAGCCAA 
ACCCGCCGGC ACACCATTAT TTCCACCACG CTGACCAACT ACAATCAGCA GCTGGCGGCC 
GCATTTCCCG ATGCTACGCG ACGCTCCTCC ATCGGTTGGG ATACCACTCC GACCACGCCC 
ACCCCGCATG GCAACGCCTC CAAGCCGGTA AGGATTACGC TAATCGGCGA GGCACAGCCG 
CTGCAGAGCA AGTCATCAAC CGGATCGCTA TGCTCGTCAT CATCATCGTC GTCGTCATCC 
TCCTCATCAT CAACATCCTT GTTGGCACAA AATGCCAATG TGGTCAAGAT GCGATATACC 
AATGGCTGTC AGAGTGTTAA GAACGGATCG AGTGCCCATT ATCAGCCGTT GTACAACAGC 
AACAAGATGA CAAGCCCAAC CGTAACTATA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_001274721.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261650.1 Drosophila melanogaster uncharacterized protein, transcript variant K (CG42268), mRNA.

Price & Availability↑

CloneID	OFa20807
Clone ID Related Accession (Same CDS sequence)	NM_001274720.1 , NM_001274721.1
Accession Version	NM_001274721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTATG ACAAGTCCTT CCGCAATGCG ATGATCCAGG ATGTGCTGCA GTTCAAGAAG 
CAGCTGCTCC GACTGCGGCG AATACTCCAG GAGACGGAAA CGCTCAATCC CTTCGAGAAC 
GATAACGTCC AGCTGTTCGC CGCCTGCGGA TTGGATAGCA AGCAATTGAA TGACATCGAT 
TTGGCCAGCC TAACCTCGTC CACGACGGAG GATCCGCTAC AGGAGCTAAC GGATCTACGT 
AGACAGGTGG TTTACCTTCA GGGTCAAGTG GACGATCGTG ATCGCACCAT TCGCCTGCAG 
CGCGATCTCA TCGAGCAACT GGAGGCCGAG AAGAGGCAGA AGGCTTCGCA TGGACCGGGA 
TCTGGCGCGG ATCCGGGCAA GGAGCTAATC AGCATGGCCA CCCAAACGGA GCGGACACGA 
CCACTTGCCA TTGGTGCGGA GGGTTTGTCC AGAAGTAAGC CCGAATATAC CAGTTATACC 
ACGCACTTTC CGATGCTCCA CTTGCACGAT TCCACGCTGG CAGCCACCAC GATAATCCGT 
CATCATCAGA GCAACCATGA GCAGCAACAG CCGGAGAAAT CCTGTCCAGC TCTCAGCCAA 
ACCCGCCGGC ACACCATTAT TTCCACCACG CTGACCAACT ACAATCAGCA GCTGGCGGCC 
GCATTTCCCG ATGCTACGCG ACGCTCCTCC ATCGGTTGGG ATACCACTCC GACCACGCCC 
ACCCCGCATG GCAACGCCTC CAAGCCGGTA AGGATTACGC TAATCGGCGA GGCACAGCCG 
CTGCAGAGCA AGTCATCAAC CGGATCGCTA TGCTCGTCAT CATCATCGTC GTCGTCATCC 
TCCTCATCAT CAACATCCTT GTTGGCACAA AATGCCAATG TGGTCAAGAT GCGATATACC 
AATGGCTGTC AGAGTGTTAA GAACGGATCG AGTGCCCATT ATCAGCCGTT GTACAACAGC 
AACAAGATGA CAAGCCCAAC CGTAACTATA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261650.1
CDS	532..1527
Translation	MLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQVVYLQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_001274721.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant K (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274721.1

ORF Insert Sequence:

ATGTTGTATG ACAAGTCCTT CCGCAATGCG ATGATCCAGG ATGTGCTGCA GTTCAAGAAG 
CAGCTGCTCC GACTGCGGCG AATACTCCAG GAGACGGAAA CGCTCAATCC CTTCGAGAAC 
GATAACGTCC AGCTGTTCGC CGCCTGCGGA TTGGATAGCA AGCAATTGAA TGACATCGAT 
TTGGCCAGCC TAACCTCGTC CACGACGGAG GATCCGCTAC AGGAGCTAAC GGATCTACGT 
AGACAGGTGG TTTACCTTCA GGGTCAAGTG GACGATCGTG ATCGCACCAT TCGCCTGCAG 
CGCGATCTCA TCGAGCAACT GGAGGCCGAG AAGAGGCAGA AGGCTTCGCA TGGACCGGGA 
TCTGGCGCGG ATCCGGGCAA GGAGCTAATC AGCATGGCCA CCCAAACGGA GCGGACACGA 
CCACTTGCCA TTGGTGCGGA GGGTTTGTCC AGAAGTAAGC CCGAATATAC CAGTTATACC 
ACGCACTTTC CGATGCTCCA CTTGCACGAT TCCACGCTGG CAGCCACCAC GATAATCCGT 
CATCATCAGA GCAACCATGA GCAGCAACAG CCGGAGAAAT CCTGTCCAGC TCTCAGCCAA 
ACCCGCCGGC ACACCATTAT TTCCACCACG CTGACCAACT ACAATCAGCA GCTGGCGGCC 
GCATTTCCCG ATGCTACGCG ACGCTCCTCC ATCGGTTGGG ATACCACTCC GACCACGCCC 
ACCCCGCATG GCAACGCCTC CAAGCCGGTA AGGATTACGC TAATCGGCGA GGCACAGCCG 
CTGCAGAGCA AGTCATCAAC CGGATCGCTA TGCTCGTCAT CATCATCGTC GTCGTCATCC 
TCCTCATCAT CAACATCCTT GTTGGCACAA AATGCCAATG TGGTCAAGAT GCGATATACC 
AATGGCTGTC AGAGTGTTAA GAACGGATCG AGTGCCCATT ATCAGCCGTT GTACAACAGC 
AACAAGATGA CAAGCCCAAC CGTAACTATA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_168366.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729542.4 Drosophila melanogaster uncharacterized protein, transcript variant H (CG42268), mRNA.

Price & Availability↑

CloneID	OFa18097
Clone ID Related Accession (Same CDS sequence)	NM_168366.4
Accession Version	NM_168366.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_168366.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG GTCAAGTGGA CGATCGTGAT CGCACCATTC GCCTGCAGCG CGATCTCATC 
GAGCAACTGG AGGCCGAGAA GAGGCAGAAG GCTTCGCATG GACCGGGATC TGGCGCGGAT 
CCGGGCAAGG AGCTAATCAG CATGGCCACC CAAACGGAGC GGACACGACC ACTTGCCATT 
GGTGCGGAGG GTTTGTCCAG ACTACAATTT GGGGAGCAAC AGAGTCCCAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729542.4
CDS	455..4111
Translation	MDECDNPVVVSLGKLESQQLNEGVAEPSAENSDQEQALCDYKEPNIDDKEALIEQDLHKSSVEEDAANDAEVEAVIMGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRLQFGEQQSPKK

Target ORF information:

RefSeq Version	NM_168366.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant H (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168366.4

ORF Insert Sequence:

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG GTCAAGTGGA CGATCGTGAT CGCACCATTC GCCTGCAGCG CGATCTCATC 
GAGCAACTGG AGGCCGAGAA GAGGCAGAAG GCTTCGCATG GACCGGGATC TGGCGCGGAT 
CCGGGCAAGG AGCTAATCAG CATGGCCACC CAAACGGAGC GGACACGACC ACTTGCCATT 
GGTGCGGAGG GTTTGTCCAG ACTACAATTT GGGGAGCAAC AGAGTCCCAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_001274723.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261652.1 Drosophila melanogaster uncharacterized protein, transcript variant M (CG42268), mRNA.

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CloneID	OFa20822
Clone ID Related Accession (Same CDS sequence)	NM_001274723.1
Accession Version	NM_001274723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG TGGTTTACCT TCAGGGTCAA GTGGACGATC GTGATCGCAC CATTCGCCTG 
CAGCGCGATC TCATCGAGCA ACTGGAGGCC GAGAAGAGGC AGAAGGCTTC GCATGGACCG 
GGATCTGGCG CGGATCCGGG CAAGGAGCTA ATCAGCATGG CCACCCAAAC GGAGCGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261652.1
CDS	455..4057
Translation	MDECDNPVVVSLGKLESQQLNEGVAEPSAENSDQEQALCDYKEPNIDDKEALIEQDLHKSSVEEDAANDAEVEAVIMGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQVVYLQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERK

Target ORF information:

RefSeq Version	NM_001274723.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant M (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274723.1

ORF Insert Sequence:

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG TGGTTTACCT TCAGGGTCAA GTGGACGATC GTGATCGCAC CATTCGCCTG 
CAGCGCGATC TCATCGAGCA ACTGGAGGCC GAGAAGAGGC AGAAGGCTTC GCATGGACCG 
GGATCTGGCG CGGATCCGGG CAAGGAGCTA ATCAGCATGG CCACCCAAAC GGAGCGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_168368.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729544.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG42268), mRNA.

Price & Availability↑

CloneID	OFa18096
Clone ID Related Accession (Same CDS sequence)	NM_168368.2
Accession Version	NM_168368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 26, 2009 this sequence version replaced NM_168368.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGT GTGTCTCCCG GCAGTCGGCT CCGCTCCACG AACTCGCATC CCCAGACGCG 
CAGCATCATC AATCTGTATT GACAATCGCA CTGTCCCACG AAGATTTGGC CCAGGCACTC 
AAAATCTGGC AACAGTTGAC AGGGAGTAAT GAATACATTG CAGCCGGGCC ATCCTCGCAC 
CAGGAGGAGG AACCCTTTTA TTACACTATA AGGCGGCAAA GGCAACCGGA GCAACAGCAG 
CAGCGGGAGC AGGAGCAGGA GCAGGCCGTG TTGCATATCG AGTCCCTTTA CAATGATGCC 
GCCATCGCCC CAGCGGAACC AGTGACATCA GCCAGCGAAC CGGAGTACTC CACTTGCCAG 
GAATTCCTGC TGCACGAACT GCTGCAGGTC ATCGAGGAAC ATGCAGCCTC CACGACGACG 
ACGACCAACC ACATCCTGAA TCAGTCTCGA ATCAGGCCAG AACCTCAATT ACCCGTCGAG 
AACAACGGCT GGCTGGCGGT GGGACAGGCG GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT 
CGCCGCCACC ACAATGGCAA CGGAAATAGT AATGGCAATC CCACGGAGGC GATGGAACAG 
AATCAGACGG TGCACACCAA AGGCACCGCC ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT 
GGGACGCCCA AGAAGTTCAA GAAACTCCTG GAAGGCGGCG ACAAAAGCGC AACTCGCACA 
GACACAAAGG ATGACAACGG AAATGCAGCT GTTCCCATTC ACTTCGAGAA AGTAGGCGCC 
GCCGCTGTAC AGAAGGCTGG GACTTTGGCG ACAGGTGCGG CCGGCGGACG TTTCGGTTAT 
CGTGGGGCGG TGCCACGCCC CTCTTCTGCA GGCTTCACTG CGCCCAGTGA AGATTCCGAA 
TCGGAATCGA TGGCCAATGC CCAAAACAAC ATCAACAACA ACAATAACAA TAATAATGGA 
CGTGGAACGG AGAATGGTCC GGTGTTGGTG AGCAATTTGA AACGACGCTC CAAGAGCGCA 
CACGCCGGAC GATCCGGCGA TGGGGAGCCC AAAATAGCCC AGCCCAAGAC GCTGACGTTC 
AACCTGAATC AGAACACCAC CATCGAGTAC CAGCGGCGTC AGTTCTTCGG CGAGATCGCG 
GACGAAACGT CGGGTTCCGG ATCCGGATCG GGAACGGGAT CGAGAGCTAT GCAACCGAGA 
TATAACTACA ACAACCTGGC CAGCATGCAT GCCAATGTCA TAGTTCGCCC CACACCGCGA 
CCCACTCCAG CCAGCTATGC CAAGTTCACA CTGCAGACAG TGAGCCTGCC CAGGCCGGAG 
TACCCGGTGG CCATCAGTTT GACAGCCACC ACACCAACCA CGCCCAGCAG CAGTGTGCAG 
TCACCTGTGC CCGCCCATTC GACAGGTGCC AGGGCCAAGG ACATCTCGAC CAGTTCCAGG 
CAACATCCTT TGACCTCGGT CCATGTCCAA CCACAGTCGC GTCATCTTGA CCAGAAGAGC 
GTGAAGCAGC TAACGAATAA CTCCACACGA CGAGGATTCT CCGGAAGCAG GGAGATTAGC 
GCCGATTCTG GGATAGCCAG CATGGACATG GCTTTGGACA GCAGTTCGGG CAGCTCGGTG 
GGTTCCAAGA GGAGTCGGAG CAGACCAAGG AACCTAAAGA TGGTGATGAG TGGACGGCAC 
ACATTCGAGG TGCGCGATGC GGATGATCCG CCATCCAGTG AGTCCAACTC CTTTGTGGAA 
CCACTGGCAC TACCAAAGTT ACCCACTGAT GGAAGTCAGA GTATTCCTCT GCCATTGCTG 
GGCTTGGTGC GATCCAATAC GGTGTTGAGT CGGGAGTCTT ACGAGCGACG TCAGGCGGAA 
ACACCAGGAT CCCAGGATCA GCAGGATTCG GAAAAACCCA AAACCAGCGG CTCCGATGAA 
AGCGAGAGTG TGGATGAAGA GAAACTCTAC TTGGACTCGT CCACCTCCGA AAAGAGTGCC 
AAACATCAGA GCCAATCGTC AGTGGCTTCC ACTTGGCGTC ACCAGGCGGG TGAATCTCTG 
GCCGCCCAGG ATTGCAGCAG CATGAGCATG AGCATCAGTA GTGACACCCA TGCTCCGGAA 
AATCCGCGCG ATAATGACAA GGAGGAGGAC ATGTCCTTGG GTCTGGATGA GATCAGTCTG 
ATCAACACTG ACATGCAGTT TAGCACAATC TCTTCAATGA CGGAAACTCC ACCCAAAGTG 
GGTCAGGAGT CCCTGCTCAA TCTTCACCTG GTGGACAATC GGGAGGCAGG CACCTCGCGT 
CCCCGCTCCT TTAACAATGC TCTTAATGAA TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT 
AGTAGTTGCC TCCTGTTGGA TGATGAGACA TCGCCCACAG ACAGTTTGGT CAGCAGCACC 
GAAGATTCCG AGGAGGCTGG TGGCAAGTTG CAAAAGCACA AGCTCAATGA GGAGCGTCAG 
CAGAAGGACA TCGATGACAT CGACATAGAT GATATATCAC CGGTTCTCGA ACTGGATCTG 
GATCCTCCAG GTGGACGAAG TCCCATTTCA CCCGGCACTC CCACGCATGC ATCCCATTCC 
CTTTCTTTGG GTTCGGATTG CGGAAATCTT ATTGATGATG AGATCGCCGA TCAGCCAGCA 
TTGCTATGCA ACAGTGAAGC CCATGAGGTG GCCACCGATA CGCCCACATT GATGGAAACC 
CTCACGCACA CCCAAACAGG ATCCTTGAGA TCCTTGAAGA GTCAGTCGAA GGCACGTACC 
GCTCTGCAGC AGGCCATCGA GTTGAGTTTA AGGACTCCGG CAGCGGTACG AAAGGCAGTT 
ATGGATCGAG CCGAATCCTT GGATACTCTA TCGCCATGCG AATCCATTTG CTCCGATGAC 
CTGATGATGG ACTTCGATAT GAACAGCAGC GTGGACTCGA TCGATCACAT GGCCAGTTCA 
ACGGGACGCA GTCGCAGTGG CTCGGATCTT CACAAGATTG GTGGTCAGGA TATCGATCCC 
ATGCAGGCGG AAACTGAGGC GGAACTTCTA TCCGAGTTGG AACGCAGGGG CAGTGATGTG 
ATGAAGGAGC TCAATACTTT GCTGCGAGGA AGGAGGCAGC GCGGAGGACC AAGGGAGAGG 
ATTAGCGCCC AACTGCCTGC GCGTGCCACC AGATTGCTGA ACCGCTCACG TCTGCAGGAC 
CAACAACTCA CCGGCAACGA TTCGGATAAC AGCTTGAGGT CCTCCCACAG CGGCGGAGCT 
TCGGCGGCGG CCAGGAAGCG AAGCACGACC AACTCAAGGA CCTCGTCGGG TTCCACGGCC 
AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG CAGCATCTGG GATCGGGTGG CGGAGCAGGA 
GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC ACTGCGTCCG GTACATCCAC CCAGAGGTGC 
GGGGGAGAGC TGCACAGCTC GTCGGACGAT CTAATGTTGT ATGACAAGTC CTTCCGCAAT 
GCGATGATCC AGGATGTGCT GCAGTTCAAG AAGCAGCTGC TCCGACTGCG GCGAATACTC 
CAGGAGACGG AAACGCTCAA TCCCTTCGAG AACGATAACG TCCAGCTGTT CGCCGCCTGC 
GGATTGGATA GCAAGCAATT GAATGACATC GATTTGGCCA GCCTAACCTC GTCCACGACG 
GAGGATCCGC TACAGGAGCT AACGGATCTA CGTAGACAGG GTCAAGTGGA CGATCGTGAT 
CGCACCATTC GCCTGCAGCG CGATCTCATC GAGCAACTGG AGGCCGAGAA GAGGCAGAAG 
GCTTCGCATG GACCGGGATC TGGCGCGGAT CCGGGCAAGG AGCTAATCAG CATGGCCACC 
CAAACGGAGC GGACACGACC ACTTGCCATT GGTGCGGAGG GTTTGTCCAG AAGTAAGCCC 
GAATATACCA GTTATACCAC GCACTTTCCG ATGCTCCACT TGCACGATTC CACGCTGGCA 
GCCACCACGA TAATCCGTCA TCATCAGAGC AACCATGAGC AGCAACAGCC GGAGAAATCC 
TGTCCAGCTC TCAGCCAAAC CCGCCGGCAC ACCATTATTT CCACCACGCT GACCAACTAC 
AATCAGCAGC TGGCGGCCGC ATTTCCCGAT GCTACGCGAC GCTCCTCCAT CGGTTGGGAT 
ACCACTCCGA CCACGCCCAC CCCGCATGGC AACGCCTCCA AGCCGGTAAG GATTACGCTA 
ATCGGCGAGG CACAGCCGCT GCAGAGCAAG TCATCAACCG GATCGCTATG CTCGTCATCA 
TCATCGTCGT CGTCATCCTC CTCATCATCA ACATCCTTGT TGGCACAAAA TGCCAATGTG 
GTCAAGATGC GATATACCAA TGGCTGTCAG AGTGTTAAGA ACGGATCGAG TGCCCATTAT 
CAGCCGTTGT ACAACAGCAA CAAGATGACA AGCCCAACCG TAACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729544.2
CDS	506..4939
Translation	MGKCVSRQSAPLHELASPDAQHHQSVLTIALSHEDLAQALKIWQQLTGSNEYIAAGPSSHQEEEPFYYTIRRQRQPEQQQQREQEQEQAVLHIESLYNDAAIAPAEPVTSASEPEYSTCQEFLLHELLQVIEEHAASTTTTTNHILNQSRIRPEPQLPVENNGWLAVGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_168368.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168368.2

ORF Insert Sequence:

ATGGGCAAGT GTGTCTCCCG GCAGTCGGCT CCGCTCCACG AACTCGCATC CCCAGACGCG 
CAGCATCATC AATCTGTATT GACAATCGCA CTGTCCCACG AAGATTTGGC CCAGGCACTC 
AAAATCTGGC AACAGTTGAC AGGGAGTAAT GAATACATTG CAGCCGGGCC ATCCTCGCAC 
CAGGAGGAGG AACCCTTTTA TTACACTATA AGGCGGCAAA GGCAACCGGA GCAACAGCAG 
CAGCGGGAGC AGGAGCAGGA GCAGGCCGTG TTGCATATCG AGTCCCTTTA CAATGATGCC 
GCCATCGCCC CAGCGGAACC AGTGACATCA GCCAGCGAAC CGGAGTACTC CACTTGCCAG 
GAATTCCTGC TGCACGAACT GCTGCAGGTC ATCGAGGAAC ATGCAGCCTC CACGACGACG 
ACGACCAACC ACATCCTGAA TCAGTCTCGA ATCAGGCCAG AACCTCAATT ACCCGTCGAG 
AACAACGGCT GGCTGGCGGT GGGACAGGCG GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT 
CGCCGCCACC ACAATGGCAA CGGAAATAGT AATGGCAATC CCACGGAGGC GATGGAACAG 
AATCAGACGG TGCACACCAA AGGCACCGCC ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT 
GGGACGCCCA AGAAGTTCAA GAAACTCCTG GAAGGCGGCG ACAAAAGCGC AACTCGCACA 
GACACAAAGG ATGACAACGG AAATGCAGCT GTTCCCATTC ACTTCGAGAA AGTAGGCGCC 
GCCGCTGTAC AGAAGGCTGG GACTTTGGCG ACAGGTGCGG CCGGCGGACG TTTCGGTTAT 
CGTGGGGCGG TGCCACGCCC CTCTTCTGCA GGCTTCACTG CGCCCAGTGA AGATTCCGAA 
TCGGAATCGA TGGCCAATGC CCAAAACAAC ATCAACAACA ACAATAACAA TAATAATGGA 
CGTGGAACGG AGAATGGTCC GGTGTTGGTG AGCAATTTGA AACGACGCTC CAAGAGCGCA 
CACGCCGGAC GATCCGGCGA TGGGGAGCCC AAAATAGCCC AGCCCAAGAC GCTGACGTTC 
AACCTGAATC AGAACACCAC CATCGAGTAC CAGCGGCGTC AGTTCTTCGG CGAGATCGCG 
GACGAAACGT CGGGTTCCGG ATCCGGATCG GGAACGGGAT CGAGAGCTAT GCAACCGAGA 
TATAACTACA ACAACCTGGC CAGCATGCAT GCCAATGTCA TAGTTCGCCC CACACCGCGA 
CCCACTCCAG CCAGCTATGC CAAGTTCACA CTGCAGACAG TGAGCCTGCC CAGGCCGGAG 
TACCCGGTGG CCATCAGTTT GACAGCCACC ACACCAACCA CGCCCAGCAG CAGTGTGCAG 
TCACCTGTGC CCGCCCATTC GACAGGTGCC AGGGCCAAGG ACATCTCGAC CAGTTCCAGG 
CAACATCCTT TGACCTCGGT CCATGTCCAA CCACAGTCGC GTCATCTTGA CCAGAAGAGC 
GTGAAGCAGC TAACGAATAA CTCCACACGA CGAGGATTCT CCGGAAGCAG GGAGATTAGC 
GCCGATTCTG GGATAGCCAG CATGGACATG GCTTTGGACA GCAGTTCGGG CAGCTCGGTG 
GGTTCCAAGA GGAGTCGGAG CAGACCAAGG AACCTAAAGA TGGTGATGAG TGGACGGCAC 
ACATTCGAGG TGCGCGATGC GGATGATCCG CCATCCAGTG AGTCCAACTC CTTTGTGGAA 
CCACTGGCAC TACCAAAGTT ACCCACTGAT GGAAGTCAGA GTATTCCTCT GCCATTGCTG 
GGCTTGGTGC GATCCAATAC GGTGTTGAGT CGGGAGTCTT ACGAGCGACG TCAGGCGGAA 
ACACCAGGAT CCCAGGATCA GCAGGATTCG GAAAAACCCA AAACCAGCGG CTCCGATGAA 
AGCGAGAGTG TGGATGAAGA GAAACTCTAC TTGGACTCGT CCACCTCCGA AAAGAGTGCC 
AAACATCAGA GCCAATCGTC AGTGGCTTCC ACTTGGCGTC ACCAGGCGGG TGAATCTCTG 
GCCGCCCAGG ATTGCAGCAG CATGAGCATG AGCATCAGTA GTGACACCCA TGCTCCGGAA 
AATCCGCGCG ATAATGACAA GGAGGAGGAC ATGTCCTTGG GTCTGGATGA GATCAGTCTG 
ATCAACACTG ACATGCAGTT TAGCACAATC TCTTCAATGA CGGAAACTCC ACCCAAAGTG 
GGTCAGGAGT CCCTGCTCAA TCTTCACCTG GTGGACAATC GGGAGGCAGG CACCTCGCGT 
CCCCGCTCCT TTAACAATGC TCTTAATGAA TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT 
AGTAGTTGCC TCCTGTTGGA TGATGAGACA TCGCCCACAG ACAGTTTGGT CAGCAGCACC 
GAAGATTCCG AGGAGGCTGG TGGCAAGTTG CAAAAGCACA AGCTCAATGA GGAGCGTCAG 
CAGAAGGACA TCGATGACAT CGACATAGAT GATATATCAC CGGTTCTCGA ACTGGATCTG 
GATCCTCCAG GTGGACGAAG TCCCATTTCA CCCGGCACTC CCACGCATGC ATCCCATTCC 
CTTTCTTTGG GTTCGGATTG CGGAAATCTT ATTGATGATG AGATCGCCGA TCAGCCAGCA 
TTGCTATGCA ACAGTGAAGC CCATGAGGTG GCCACCGATA CGCCCACATT GATGGAAACC 
CTCACGCACA CCCAAACAGG ATCCTTGAGA TCCTTGAAGA GTCAGTCGAA GGCACGTACC 
GCTCTGCAGC AGGCCATCGA GTTGAGTTTA AGGACTCCGG CAGCGGTACG AAAGGCAGTT 
ATGGATCGAG CCGAATCCTT GGATACTCTA TCGCCATGCG AATCCATTTG CTCCGATGAC 
CTGATGATGG ACTTCGATAT GAACAGCAGC GTGGACTCGA TCGATCACAT GGCCAGTTCA 
ACGGGACGCA GTCGCAGTGG CTCGGATCTT CACAAGATTG GTGGTCAGGA TATCGATCCC 
ATGCAGGCGG AAACTGAGGC GGAACTTCTA TCCGAGTTGG AACGCAGGGG CAGTGATGTG 
ATGAAGGAGC TCAATACTTT GCTGCGAGGA AGGAGGCAGC GCGGAGGACC AAGGGAGAGG 
ATTAGCGCCC AACTGCCTGC GCGTGCCACC AGATTGCTGA ACCGCTCACG TCTGCAGGAC 
CAACAACTCA CCGGCAACGA TTCGGATAAC AGCTTGAGGT CCTCCCACAG CGGCGGAGCT 
TCGGCGGCGG CCAGGAAGCG AAGCACGACC AACTCAAGGA CCTCGTCGGG TTCCACGGCC 
AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG CAGCATCTGG GATCGGGTGG CGGAGCAGGA 
GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC ACTGCGTCCG GTACATCCAC CCAGAGGTGC 
GGGGGAGAGC TGCACAGCTC GTCGGACGAT CTAATGTTGT ATGACAAGTC CTTCCGCAAT 
GCGATGATCC AGGATGTGCT GCAGTTCAAG AAGCAGCTGC TCCGACTGCG GCGAATACTC 
CAGGAGACGG AAACGCTCAA TCCCTTCGAG AACGATAACG TCCAGCTGTT CGCCGCCTGC 
GGATTGGATA GCAAGCAATT GAATGACATC GATTTGGCCA GCCTAACCTC GTCCACGACG 
GAGGATCCGC TACAGGAGCT AACGGATCTA CGTAGACAGG GTCAAGTGGA CGATCGTGAT 
CGCACCATTC GCCTGCAGCG CGATCTCATC GAGCAACTGG AGGCCGAGAA GAGGCAGAAG 
GCTTCGCATG GACCGGGATC TGGCGCGGAT CCGGGCAAGG AGCTAATCAG CATGGCCACC 
CAAACGGAGC GGACACGACC ACTTGCCATT GGTGCGGAGG GTTTGTCCAG AAGTAAGCCC 
GAATATACCA GTTATACCAC GCACTTTCCG ATGCTCCACT TGCACGATTC CACGCTGGCA 
GCCACCACGA TAATCCGTCA TCATCAGAGC AACCATGAGC AGCAACAGCC GGAGAAATCC 
TGTCCAGCTC TCAGCCAAAC CCGCCGGCAC ACCATTATTT CCACCACGCT GACCAACTAC 
AATCAGCAGC TGGCGGCCGC ATTTCCCGAT GCTACGCGAC GCTCCTCCAT CGGTTGGGAT 
ACCACTCCGA CCACGCCCAC CCCGCATGGC AACGCCTCCA AGCCGGTAAG GATTACGCTA 
ATCGGCGAGG CACAGCCGCT GCAGAGCAAG TCATCAACCG GATCGCTATG CTCGTCATCA 
TCATCGTCGT CGTCATCCTC CTCATCATCA ACATCCTTGT TGGCACAAAA TGCCAATGTG 
GTCAAGATGC GATATACCAA TGGCTGTCAG AGTGTTAAGA ACGGATCGAG TGCCCATTAT 
CAGCCGTTGT ACAACAGCAA CAAGATGACA AGCCCAACCG TAACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_168367.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729543.1 Drosophila melanogaster uncharacterized protein, transcript variant F (CG42268), mRNA.

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CloneID	OFa18098
Clone ID Related Accession (Same CDS sequence)	NM_168367.1
Accession Version	NM_168367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG TGGTTTACCT TCAGGGTCAA GTGGACGATC GTGATCGCAC CATTCGCCTG 
CAGCGCGATC TCATCGAGCA ACTGGAGGCC GAGAAGAGGC AGAAGGCTTC GCATGGACCG 
GGATCTGGCG CGGATCCGGG CAAGGAGCTA ATCAGCATGG CCACCCAAAC GGAGCGGACA 
CGACCACTTG CCATTGGTGC GGAGGGTTTG TCCAGAAGTA AGCCCGAATA TACCAGTTAT 
ACCACGCACT TTCCGATGCT CCACTTGCAC GATTCCACGC TGGCAGCCAC CACGATAATC 
CGTCATCATC AGAGCAACCA TGAGCAGCAA CAGCCGGAGA AATCCTGTCC AGCTCTCAGC 
CAAACCCGCC GGCACACCAT TATTTCCACC ACGCTGACCA ACTACAATCA GCAGCTGGCG 
GCCGCATTTC CCGATGCTAC GCGACGCTCC TCCATCGGTT GGGATACCAC TCCGACCACG 
CCCACCCCGC ATGGCAACGC CTCCAAGCCG GTAAGGATTA CGCTAATCGG CGAGGCACAG 
CCGCTGCAGA GCAAGTCATC AACCGGATCG CTATGCTCGT CATCATCATC GTCGTCGTCA 
TCCTCCTCAT CATCAACATC CTTGTTGGCA CAAAATGCCA ATGTGGTCAA GATGCGATAT 
ACCAATGGCT GTCAGAGTGT TAAGAACGGA TCGAGTGCCC ATTATCAGCC GTTGTACAAC 
AGCAACAAGA TGACAAGCCC AACCGTAACT ATAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729543.1
CDS	455..4633
Translation	MDECDNPVVVSLGKLESQQLNEGVAEPSAENSDQEQALCDYKEPNIDDKEALIEQDLHKSSVEEDAANDAEVEAVIMGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQETETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQVVYLQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_168367.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant F (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168367.1

ORF Insert Sequence:

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGACGG AAACGCTCAA TCCCTTCGAG 
AACGATAACG TCCAGCTGTT CGCCGCCTGC GGATTGGATA GCAAGCAATT GAATGACATC 
GATTTGGCCA GCCTAACCTC GTCCACGACG GAGGATCCGC TACAGGAGCT AACGGATCTA 
CGTAGACAGG TGGTTTACCT TCAGGGTCAA GTGGACGATC GTGATCGCAC CATTCGCCTG 
CAGCGCGATC TCATCGAGCA ACTGGAGGCC GAGAAGAGGC AGAAGGCTTC GCATGGACCG 
GGATCTGGCG CGGATCCGGG CAAGGAGCTA ATCAGCATGG CCACCCAAAC GGAGCGGACA 
CGACCACTTG CCATTGGTGC GGAGGGTTTG TCCAGAAGTA AGCCCGAATA TACCAGTTAT 
ACCACGCACT TTCCGATGCT CCACTTGCAC GATTCCACGC TGGCAGCCAC CACGATAATC 
CGTCATCATC AGAGCAACCA TGAGCAGCAA CAGCCGGAGA AATCCTGTCC AGCTCTCAGC 
CAAACCCGCC GGCACACCAT TATTTCCACC ACGCTGACCA ACTACAATCA GCAGCTGGCG 
GCCGCATTTC CCGATGCTAC GCGACGCTCC TCCATCGGTT GGGATACCAC TCCGACCACG 
CCCACCCCGC ATGGCAACGC CTCCAAGCCG GTAAGGATTA CGCTAATCGG CGAGGCACAG 
CCGCTGCAGA GCAAGTCATC AACCGGATCG CTATGCTCGT CATCATCATC GTCGTCGTCA 
TCCTCCTCAT CATCAACATC CTTGTTGGCA CAAAATGCCA ATGTGGTCAA GATGCGATAT 
ACCAATGGCT GTCAGAGTGT TAAGAACGGA TCGAGTGCCC ATTATCAGCC GTTGTACAAC 
AGCAACAAGA TGACAAGCCC AACCGTAACT ATAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_001274722.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261651.1 Drosophila melanogaster uncharacterized protein, transcript variant L (CG42268), mRNA.

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CloneID	OFa20806
Clone ID Related Accession (Same CDS sequence)	NM_001274722.1
Accession Version	NM_001274722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGCCCC AAAAGGAGCC AGATTTTGAT 
TTGAAGCTGA CGGAAACGCT CAATCCCTTC GAGAACGATA ACGTCCAGCT GTTCGCCGCC 
TGCGGATTGG ATAGCAAGCA ATTGAATGAC ATCGATTTGG CCAGCCTAAC CTCGTCCACG 
ACGGAGGATC CGCTACAGGA GCTAACGGAT CTACGTAGAC AGGGTCAAGT GGACGATCGT 
GATCGCACCA TTCGCCTGCA GCGCGATCTC ATCGAGCAAC TGGAGGCCGA GAAGAGGCAG 
AAGGCTTCGC ATGGACCGGG ATCTGGCGCG GATCCGGGCA AGGAGCTAAT CAGCATGGCC 
ACCCAAACGG AGCGGACACG ACCACTTGCC ATTGGTGCGG AGGGTTTGTC CAGAAGTAAG 
CCCGAATATA CCAGTTATAC CACGCACTTT CCGATGCTCC ACTTGCACGA TTCCACGCTG 
GCAGCCACCA CGATAATCCG TCATCATCAG AGCAACCATG AGCAGCAACA GCCGGAGAAA 
TCCTGTCCAG CTCTCAGCCA AACCCGCCGG CACACCATTA TTTCCACCAC GCTGACCAAC 
TACAATCAGC AGCTGGCGGC CGCATTTCCC GATGCTACGC GACGCTCCTC CATCGGTTGG 
GATACCACTC CGACCACGCC CACCCCGCAT GGCAACGCCT CCAAGCCGGT AAGGATTACG 
CTAATCGGCG AGGCACAGCC GCTGCAGAGC AAGTCATCAA CCGGATCGCT ATGCTCGTCA 
TCATCATCGT CGTCGTCATC CTCCTCATCA TCAACATCCT TGTTGGCACA AAATGCCAAT 
GTGGTCAAGA TGCGATATAC CAATGGCTGT CAGAGTGTTA AGAACGGATC GAGTGCCCAT 
TATCAGCCGT TGTACAACAG CAACAAGATG ACAAGCCCAA CCGTAACTAT AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261651.1
CDS	455..4651
Translation	MDECDNPVVVSLGKLESQQLNEGVAEPSAENSDQEQALCDYKEPNIDDKEALIEQDLHKSSVEEDAANDAEVEAVIMGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQEPQKEPDFDLKLTETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_001274722.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant L (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274722.1

ORF Insert Sequence:

ATGGATGAAT GCGACAACCC CGTCGTCGTC TCACTAGGAA AACTCGAATC ACAGCAGCTA 
AACGAGGGGG TCGCCGAGCC GAGTGCGGAA AATTCGGATC AGGAGCAGGC ACTTTGTGAC 
TACAAAGAGC CGAACATTGA TGATAAGGAA GCGCTTATAG AGCAGGACCT TCACAAATCA 
TCAGTCGAGG AGGACGCAGC TAACGACGCG GAAGTCGAGG CAGTCATCAT GGGACAGGCG 
GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT CGCCGCCACC ACAATGGCAA CGGAAATAGT 
AATGGCAATC CCACGGAGGC GATGGAACAG AATCAGACGG TGCACACCAA AGGCACCGCC 
ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT GGGACGCCCA AGAAGTTCAA GAAACTCCTG 
GAAGGCGGCG ACAAAAGCGC AACTCGCACA GACACAAAGG ATGACAACGG AAATGCAGCT 
GTTCCCATTC ACTTCGAGAA AGTAGGCGCC GCCGCTGTAC AGAAGGCTGG GACTTTGGCG 
ACAGGTGCGG CCGGCGGACG TTTCGGTTAT CGTGGGGCGG TGCCACGCCC CTCTTCTGCA 
GGCTTCACTG CGCCCAGTGA AGATTCCGAA TCGGAATCGA TGGCCAATGC CCAAAACAAC 
ATCAACAACA ACAATAACAA TAATAATGGA CGTGGAACGG AGAATGGTCC GGTGTTGGTG 
AGCAATTTGA AACGACGCTC CAAGAGCGCA CACGCCGGAC GATCCGGCGA TGGGGAGCCC 
AAAATAGCCC AGCCCAAGAC GCTGACGTTC AACCTGAATC AGAACACCAC CATCGAGTAC 
CAGCGGCGTC AGTTCTTCGG CGAGATCGCG GACGAAACGT CGGGTTCCGG ATCCGGATCG 
GGAACGGGAT CGAGAGCTAT GCAACCGAGA TATAACTACA ACAACCTGGC CAGCATGCAT 
GCCAATGTCA TAGTTCGCCC CACACCGCGA CCCACTCCAG CCAGCTATGC CAAGTTCACA 
CTGCAGACAG TGAGCCTGCC CAGGCCGGAG TACCCGGTGG CCATCAGTTT GACAGCCACC 
ACACCAACCA CGCCCAGCAG CAGTGTGCAG TCACCTGTGC CCGCCCATTC GACAGGTGCC 
AGGGCCAAGG ACATCTCGAC CAGTTCCAGG CAACATCCTT TGACCTCGGT CCATGTCCAA 
CCACAGTCGC GTCATCTTGA CCAGAAGAGC GTGAAGCAGC TAACGAATAA CTCCACACGA 
CGAGGATTCT CCGGAAGCAG GGAGATTAGC GCCGATTCTG GGATAGCCAG CATGGACATG 
GCTTTGGACA GCAGTTCGGG CAGCTCGGTG GGTTCCAAGA GGAGTCGGAG CAGACCAAGG 
AACCTAAAGA TGGTGATGAG TGGACGGCAC ACATTCGAGG TGCGCGATGC GGATGATCCG 
CCATCCAGTG AGTCCAACTC CTTTGTGGAA CCACTGGCAC TACCAAAGTT ACCCACTGAT 
GGAAGTCAGA GTATTCCTCT GCCATTGCTG GGCTTGGTGC GATCCAATAC GGTGTTGAGT 
CGGGAGTCTT ACGAGCGACG TCAGGCGGAA ACACCAGGAT CCCAGGATCA GCAGGATTCG 
GAAAAACCCA AAACCAGCGG CTCCGATGAA AGCGAGAGTG TGGATGAAGA GAAACTCTAC 
TTGGACTCGT CCACCTCCGA AAAGAGTGCC AAACATCAGA GCCAATCGTC AGTGGCTTCC 
ACTTGGCGTC ACCAGGCGGG TGAATCTCTG GCCGCCCAGG ATTGCAGCAG CATGAGCATG 
AGCATCAGTA GTGACACCCA TGCTCCGGAA AATCCGCGCG ATAATGACAA GGAGGAGGAC 
ATGTCCTTGG GTCTGGATGA GATCAGTCTG ATCAACACTG ACATGCAGTT TAGCACAATC 
TCTTCAATGA CGGAAACTCC ACCCAAAGTG GGTCAGGAGT CCCTGCTCAA TCTTCACCTG 
GTGGACAATC GGGAGGCAGG CACCTCGCGT CCCCGCTCCT TTAACAATGC TCTTAATGAA 
TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT AGTAGTTGCC TCCTGTTGGA TGATGAGACA 
TCGCCCACAG ACAGTTTGGT CAGCAGCACC GAAGATTCCG AGGAGGCTGG TGGCAAGTTG 
CAAAAGCACA AGCTCAATGA GGAGCGTCAG CAGAAGGACA TCGATGACAT CGACATAGAT 
GATATATCAC CGGTTCTCGA ACTGGATCTG GATCCTCCAG GTGGACGAAG TCCCATTTCA 
CCCGGCACTC CCACGCATGC ATCCCATTCC CTTTCTTTGG GTTCGGATTG CGGAAATCTT 
ATTGATGATG AGATCGCCGA TCAGCCAGCA TTGCTATGCA ACAGTGAAGC CCATGAGGTG 
GCCACCGATA CGCCCACATT GATGGAAACC CTCACGCACA CCCAAACAGG ATCCTTGAGA 
TCCTTGAAGA GTCAGTCGAA GGCACGTACC GCTCTGCAGC AGGCCATCGA GTTGAGTTTA 
AGGACTCCGG CAGCGGTACG AAAGGCAGTT ATGGATCGAG CCGAATCCTT GGATACTCTA 
TCGCCATGCG AATCCATTTG CTCCGATGAC CTGATGATGG ACTTCGATAT GAACAGCAGC 
GTGGACTCGA TCGATCACAT GGCCAGTTCA ACGGGACGCA GTCGCAGTGG CTCGGATCTT 
CACAAGATTG GTGGTCAGGA TATCGATCCC ATGCAGGCGG AAACTGAGGC GGAACTTCTA 
TCCGAGTTGG AACGCAGGGG CAGTGATGTG ATGAAGGAGC TCAATACTTT GCTGCGAGGA 
AGGAGGCAGC GCGGAGGACC AAGGGAGAGG ATTAGCGCCC AACTGCCTGC GCGTGCCACC 
AGATTGCTGA ACCGCTCACG TCTGCAGGAC CAACAACTCA CCGGCAACGA TTCGGATAAC 
AGCTTGAGGT CCTCCCACAG CGGCGGAGCT TCGGCGGCGG CCAGGAAGCG AAGCACGACC 
AACTCAAGGA CCTCGTCGGG TTCCACGGCC AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG 
CAGCATCTGG GATCGGGTGG CGGAGCAGGA GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC 
ACTGCGTCCG GTACATCCAC CCAGAGGTGC GGGGGAGAGC TGCACAGCTC GTCGGACGAT 
CTAATGTTGT ATGACAAGTC CTTCCGCAAT GCGATGATCC AGGATGTGCT GCAGTTCAAG 
AAGCAGCTGC TCCGACTGCG GCGAATACTC CAGGAGCCCC AAAAGGAGCC AGATTTTGAT 
TTGAAGCTGA CGGAAACGCT CAATCCCTTC GAGAACGATA ACGTCCAGCT GTTCGCCGCC 
TGCGGATTGG ATAGCAAGCA ATTGAATGAC ATCGATTTGG CCAGCCTAAC CTCGTCCACG 
ACGGAGGATC CGCTACAGGA GCTAACGGAT CTACGTAGAC AGGGTCAAGT GGACGATCGT 
GATCGCACCA TTCGCCTGCA GCGCGATCTC ATCGAGCAAC TGGAGGCCGA GAAGAGGCAG 
AAGGCTTCGC ATGGACCGGG ATCTGGCGCG GATCCGGGCA AGGAGCTAAT CAGCATGGCC 
ACCCAAACGG AGCGGACACG ACCACTTGCC ATTGGTGCGG AGGGTTTGTC CAGAAGTAAG 
CCCGAATATA CCAGTTATAC CACGCACTTT CCGATGCTCC ACTTGCACGA TTCCACGCTG 
GCAGCCACCA CGATAATCCG TCATCATCAG AGCAACCATG AGCAGCAACA GCCGGAGAAA 
TCCTGTCCAG CTCTCAGCCA AACCCGCCGG CACACCATTA TTTCCACCAC GCTGACCAAC 
TACAATCAGC AGCTGGCGGC CGCATTTCCC GATGCTACGC GACGCTCCTC CATCGGTTGG 
GATACCACTC CGACCACGCC CACCCCGCAT GGCAACGCCT CCAAGCCGGT AAGGATTACG 
CTAATCGGCG AGGCACAGCC GCTGCAGAGC AAGTCATCAA CCGGATCGCT ATGCTCGTCA 
TCATCATCGT CGTCGTCATC CTCCTCATCA TCAACATCCT TGTTGGCACA AAATGCCAAT 
GTGGTCAAGA TGCGATATAC CAATGGCTGT CAGAGTGTTA AGAACGGATC GAGTGCCCAT 
TATCAGCCGT TGTACAACAG CAACAAGATG ACAAGCCCAA CCGTAACTAT AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42268 ( NM_001274719.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261648.1 Drosophila melanogaster uncharacterized protein, transcript variant I (CG42268), mRNA.

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CloneID	OFa20820
Clone ID Related Accession (Same CDS sequence)	NM_001274719.1
Accession Version	NM_001274719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGT GTGTCTCCCG GCAGTCGGCT CCGCTCCACG AACTCGCATC CCCAGACGCG 
CAGCATCATC AATCTGTATT GACAATCGCA CTGTCCCACG AAGATTTGGC CCAGGCACTC 
AAAATCTGGC AACAGTTGAC AGGGAGTAAT GAATACATTG CAGCCGGGCC ATCCTCGCAC 
CAGGAGGAGG AACCCTTTTA TTACACTATA AGGCGGCAAA GGCAACCGGA GCAACAGCAG 
CAGCGGGAGC AGGAGCAGGA GCAGGCCGTG TTGCATATCG AGTCCCTTTA CAATGATGCC 
GCCATCGCCC CAGCGGAACC AGTGACATCA GCCAGCGAAC CGGAGTACTC CACTTGCCAG 
GAATTCCTGC TGCACGAACT GCTGCAGGTC ATCGAGGAAC ATGCAGCCTC CACGACGACG 
ACGACCAACC ACATCCTGAA TCAGTCTCGA ATCAGGCCAG AACCTCAATT ACCCGTCGAG 
AACAACGGCT GGCTGGCGGT GGGACAGGCG GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT 
CGCCGCCACC ACAATGGCAA CGGAAATAGT AATGGCAATC CCACGGAGGC GATGGAACAG 
AATCAGACGG TGCACACCAA AGGCACCGCC ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT 
GGGACGCCCA AGAAGTTCAA GAAACTCCTG GAAGGCGGCG ACAAAAGCGC AACTCGCACA 
GACACAAAGG ATGACAACGG AAATGCAGCT GTTCCCATTC ACTTCGAGAA AGTAGGCGCC 
GCCGCTGTAC AGAAGGCTGG GACTTTGGCG ACAGGTGCGG CCGGCGGACG TTTCGGTTAT 
CGTGGGGCGG TGCCACGCCC CTCTTCTGCA GGCTTCACTG CGCCCAGTGA AGATTCCGAA 
TCGGAATCGA TGGCCAATGC CCAAAACAAC ATCAACAACA ACAATAACAA TAATAATGGA 
CGTGGAACGG AGAATGGTCC GGTGTTGGTG AGCAATTTGA AACGACGCTC CAAGAGCGCA 
CACGCCGGAC GATCCGGCGA TGGGGAGCCC AAAATAGCCC AGCCCAAGAC GCTGACGTTC 
AACCTGAATC AGAACACCAC CATCGAGTAC CAGCGGCGTC AGTTCTTCGG CGAGATCGCG 
GACGAAACGT CGGGTTCCGG ATCCGGATCG GGAACGGGAT CGAGAGCTAT GCAACCGAGA 
TATAACTACA ACAACCTGGC CAGCATGCAT GCCAATGTCA TAGTTCGCCC CACACCGCGA 
CCCACTCCAG CCAGCTATGC CAAGTTCACA CTGCAGACAG TGAGCCTGCC CAGGCCGGAG 
TACCCGGTGG CCATCAGTTT GACAGCCACC ACACCAACCA CGCCCAGCAG CAGTGTGCAG 
TCACCTGTGC CCGCCCATTC GACAGGTGCC AGGGCCAAGG ACATCTCGAC CAGTTCCAGG 
CAACATCCTT TGACCTCGGT CCATGTCCAA CCACAGTCGC GTCATCTTGA CCAGAAGAGC 
GTGAAGCAGC TAACGAATAA CTCCACACGA CGAGGATTCT CCGGAAGCAG GGAGATTAGC 
GCCGATTCTG GGATAGCCAG CATGGACATG GCTTTGGACA GCAGTTCGGG CAGCTCGGTG 
GGTTCCAAGA GGAGTCGGAG CAGACCAAGG AACCTAAAGA TGGTGATGAG TGGACGGCAC 
ACATTCGAGG TGCGCGATGC GGATGATCCG CCATCCAGTG AGTCCAACTC CTTTGTGGAA 
CCACTGGCAC TACCAAAGTT ACCCACTGAT GGAAGTCAGA GTATTCCTCT GCCATTGCTG 
GGCTTGGTGC GATCCAATAC GGTGTTGAGT CGGGAGTCTT ACGAGCGACG TCAGGCGGAA 
ACACCAGGAT CCCAGGATCA GCAGGATTCG GAAAAACCCA AAACCAGCGG CTCCGATGAA 
AGCGAGAGTG TGGATGAAGA GAAACTCTAC TTGGACTCGT CCACCTCCGA AAAGAGTGCC 
AAACATCAGA GCCAATCGTC AGTGGCTTCC ACTTGGCGTC ACCAGGCGGG TGAATCTCTG 
GCCGCCCAGG ATTGCAGCAG CATGAGCATG AGCATCAGTA GTGACACCCA TGCTCCGGAA 
AATCCGCGCG ATAATGACAA GGAGGAGGAC ATGTCCTTGG GTCTGGATGA GATCAGTCTG 
ATCAACACTG ACATGCAGTT TAGCACAATC TCTTCAATGA CGGAAACTCC ACCCAAAGTG 
GGTCAGGAGT CCCTGCTCAA TCTTCACCTG GTGGACAATC GGGAGGCAGG CACCTCGCGT 
CCCCGCTCCT TTAACAATGC TCTTAATGAA TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT 
AGTAGTTGCC TCCTGTTGGA TGATGAGACA TCGCCCACAG ACAGTTTGGT CAGCAGCACC 
GAAGATTCCG AGGAGGCTGG TGGCAAGTTG CAAAAGCACA AGCTCAATGA GGAGCGTCAG 
CAGAAGGACA TCGATGACAT CGACATAGAT GATATATCAC CGGTTCTCGA ACTGGATCTG 
GATCCTCCAG GTGGACGAAG TCCCATTTCA CCCGGCACTC CCACGCATGC ATCCCATTCC 
CTTTCTTTGG GTTCGGATTG CGGAAATCTT ATTGATGATG AGATCGCCGA TCAGCCAGCA 
TTGCTATGCA ACAGTGAAGC CCATGAGGTG GCCACCGATA CGCCCACATT GATGGAAACC 
CTCACGCACA CCCAAACAGG ATCCTTGAGA TCCTTGAAGA GTCAGTCGAA GGCACGTACC 
GCTCTGCAGC AGGCCATCGA GTTGAGTTTA AGGACTCCGG CAGCGGTACG AAAGGCAGTT 
ATGGATCGAG CCGAATCCTT GGATACTCTA TCGCCATGCG AATCCATTTG CTCCGATGAC 
CTGATGATGG ACTTCGATAT GAACAGCAGC GTGGACTCGA TCGATCACAT GGCCAGTTCA 
ACGGGACGCA GTCGCAGTGG CTCGGATCTT CACAAGATTG GTGGTCAGGA TATCGATCCC 
ATGCAGGCGG AAACTGAGGC GGAACTTCTA TCCGAGTTGG AACGCAGGGG CAGTGATGTG 
ATGAAGGAGC TCAATACTTT GCTGCGAGGA AGGAGGCAGC GCGGAGGACC AAGGGAGAGG 
ATTAGCGCCC AACTGCCTGC GCGTGCCACC AGATTGCTGA ACCGCTCACG TCTGCAGGAC 
CAACAACTCA CCGGCAACGA TTCGGATAAC AGCTTGAGGT CCTCCCACAG CGGCGGAGCT 
TCGGCGGCGG CCAGGAAGCG AAGCACGACC AACTCAAGGA CCTCGTCGGG TTCCACGGCC 
AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG CAGCATCTGG GATCGGGTGG CGGAGCAGGA 
GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC ACTGCGTCCG GTACATCCAC CCAGAGGTGC 
GGGGGAGAGC TGCACAGCTC GTCGGACGAT CTAATGTTGT ATGACAAGTC CTTCCGCAAT 
GCGATGATCC AGGATGTGCT GCAGTTCAAG AAGCAGCTGC TCCGACTGCG GCGAATACTC 
CAGGAGCCCC AAAAGGAGCC AGATTTTGAT TTGAAGCTGA CGGAAACGCT CAATCCCTTC 
GAGAACGATA ACGTCCAGCT GTTCGCCGCC TGCGGATTGG ATAGCAAGCA ATTGAATGAC 
ATCGATTTGG CCAGCCTAAC CTCGTCCACG ACGGAGGATC CGCTACAGGA GCTAACGGAT 
CTACGTAGAC AGGTGGTTTA CCTTCAGGGT CAAGTGGACG ATCGTGATCG CACCATTCGC 
CTGCAGCGCG ATCTCATCGA GCAACTGGAG GCCGAGAAGA GGCAGAAGGC TTCGCATGGA 
CCGGGATCTG GCGCGGATCC GGGCAAGGAG CTAATCAGCA TGGCCACCCA AACGGAGCGG 
ACACGACCAC TTGCCATTGG TGCGGAGGGT TTGTCCAGAA GTAAGCCCGA ATATACCAGT 
TATACCACGC ACTTTCCGAT GCTCCACTTG CACGATTCCA CGCTGGCAGC CACCACGATA 
ATCCGTCATC ATCAGAGCAA CCATGAGCAG CAACAGCCGG AGAAATCCTG TCCAGCTCTC 
AGCCAAACCC GCCGGCACAC CATTATTTCC ACCACGCTGA CCAACTACAA TCAGCAGCTG 
GCGGCCGCAT TTCCCGATGC TACGCGACGC TCCTCCATCG GTTGGGATAC CACTCCGACC 
ACGCCCACCC CGCATGGCAA CGCCTCCAAG CCGGTAAGGA TTACGCTAAT CGGCGAGGCA 
CAGCCGCTGC AGAGCAAGTC ATCAACCGGA TCGCTATGCT CGTCATCATC ATCGTCGTCG 
TCATCCTCCT CATCATCAAC ATCCTTGTTG GCACAAAATG CCAATGTGGT CAAGATGCGA 
TATACCAATG GCTGTCAGAG TGTTAAGAAC GGATCGAGTG CCCATTATCA GCCGTTGTAC 
AACAGCAACA AGATGACAAG CCCAACCGTA ACTATAGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261648.1
CDS	345..4826
Translation	MGKCVSRQSAPLHELASPDAQHHQSVLTIALSHEDLAQALKIWQQLTGSNEYIAAGPSSHQEEEPFYYTIRRQRQPEQQQQREQEQEQAVLHIESLYNDAAIAPAEPVTSASEPEYSTCQEFLLHELLQVIEEHAASTTTTTNHILNQSRIRPEPQLPVENNGWLAVGQAESKKQGSKSSRRHHNGNGNSNGNPTEAMEQNQTVHTKGTAMSFGFRKKLNGTPKKFKKLLEGGDKSATRTDTKDDNGNAAVPIHFEKVGAAAVQKAGTLATGAAGGRFGYRGAVPRPSSAGFTAPSEDSESESMANAQNNINNNNNNNNGRGTENGPVLVSNLKRRSKSAHAGRSGDGEPKIAQPKTLTFNLNQNTTIEYQRRQFFGEIADETSGSGSGSGTGSRAMQPRYNYNNLASMHANVIVRPTPRPTPASYAKFTLQTVSLPRPEYPVAISLTATTPTTPSSSVQSPVPAHSTGARAKDISTSSRQHPLTSVHVQPQSRHLDQKSVKQLTNNSTRRGFSGSREISADSGIASMDMALDSSSGSSVGSKRSRSRPRNLKMVMSGRHTFEVRDADDPPSSESNSFVEPLALPKLPTDGSQSIPLPLLGLVRSNTVLSRESYERRQAETPGSQDQQDSEKPKTSGSDESESVDEEKLYLDSSTSEKSAKHQSQSSVASTWRHQAGESLAAQDCSSMSMSISSDTHAPENPRDNDKEEDMSLGLDEISLINTDMQFSTISSMTETPPKVGQESLLNLHLVDNREAGTSRPRSFNNALNESKFAELALASSSCLLLDDETSPTDSLVSSTEDSEEAGGKLQKHKLNEERQQKDIDDIDIDDISPVLELDLDPPGGRSPISPGTPTHASHSLSLGSDCGNLIDDEIADQPALLCNSEAHEVATDTPTLMETLTHTQTGSLRSLKSQSKARTALQQAIELSLRTPAAVRKAVMDRAESLDTLSPCESICSDDLMMDFDMNSSVDSIDHMASSTGRSRSGSDLHKIGGQDIDPMQAETEAELLSELERRGSDVMKELNTLLRGRRQRGGPRERISAQLPARATRLLNRSRLQDQQLTGNDSDNSLRSSHSGGASAAARKRSTTNSRTSSGSTASLPRQRHLQQQHLGSGGGAGAGGGGGGGGATASGTSTQRCGGELHSSSDDLMLYDKSFRNAMIQDVLQFKKQLLRLRRILQEPQKEPDFDLKLTETLNPFENDNVQLFAACGLDSKQLNDIDLASLTSSTTEDPLQELTDLRRQVVYLQGQVDDRDRTIRLQRDLIEQLEAEKRQKASHGPGSGADPGKELISMATQTERTRPLAIGAEGLSRSKPEYTSYTTHFPMLHLHDSTLAATTIIRHHQSNHEQQQPEKSCPALSQTRRHTIISTTLTNYNQQLAAAFPDATRRSSIGWDTTPTTPTPHGNASKPVRITLIGEAQPLQSKSSTGSLCSSSSSSSSSSSSSTSLLAQNANVVKMRYTNGCQSVKNGSSAHYQPLYNSNKMTSPTVTIV

Target ORF information:

RefSeq Version	NM_001274719.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant I (CG42268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274719.1

ORF Insert Sequence:

ATGGGCAAGT GTGTCTCCCG GCAGTCGGCT CCGCTCCACG AACTCGCATC CCCAGACGCG 
CAGCATCATC AATCTGTATT GACAATCGCA CTGTCCCACG AAGATTTGGC CCAGGCACTC 
AAAATCTGGC AACAGTTGAC AGGGAGTAAT GAATACATTG CAGCCGGGCC ATCCTCGCAC 
CAGGAGGAGG AACCCTTTTA TTACACTATA AGGCGGCAAA GGCAACCGGA GCAACAGCAG 
CAGCGGGAGC AGGAGCAGGA GCAGGCCGTG TTGCATATCG AGTCCCTTTA CAATGATGCC 
GCCATCGCCC CAGCGGAACC AGTGACATCA GCCAGCGAAC CGGAGTACTC CACTTGCCAG 
GAATTCCTGC TGCACGAACT GCTGCAGGTC ATCGAGGAAC ATGCAGCCTC CACGACGACG 
ACGACCAACC ACATCCTGAA TCAGTCTCGA ATCAGGCCAG AACCTCAATT ACCCGTCGAG 
AACAACGGCT GGCTGGCGGT GGGACAGGCG GAGAGCAAGA AGCAGGGCAG CAAGTCGAGT 
CGCCGCCACC ACAATGGCAA CGGAAATAGT AATGGCAATC CCACGGAGGC GATGGAACAG 
AATCAGACGG TGCACACCAA AGGCACCGCC ATGTCCTTTG GTTTCCGCAA AAAGCTCAAT 
GGGACGCCCA AGAAGTTCAA GAAACTCCTG GAAGGCGGCG ACAAAAGCGC AACTCGCACA 
GACACAAAGG ATGACAACGG AAATGCAGCT GTTCCCATTC ACTTCGAGAA AGTAGGCGCC 
GCCGCTGTAC AGAAGGCTGG GACTTTGGCG ACAGGTGCGG CCGGCGGACG TTTCGGTTAT 
CGTGGGGCGG TGCCACGCCC CTCTTCTGCA GGCTTCACTG CGCCCAGTGA AGATTCCGAA 
TCGGAATCGA TGGCCAATGC CCAAAACAAC ATCAACAACA ACAATAACAA TAATAATGGA 
CGTGGAACGG AGAATGGTCC GGTGTTGGTG AGCAATTTGA AACGACGCTC CAAGAGCGCA 
CACGCCGGAC GATCCGGCGA TGGGGAGCCC AAAATAGCCC AGCCCAAGAC GCTGACGTTC 
AACCTGAATC AGAACACCAC CATCGAGTAC CAGCGGCGTC AGTTCTTCGG CGAGATCGCG 
GACGAAACGT CGGGTTCCGG ATCCGGATCG GGAACGGGAT CGAGAGCTAT GCAACCGAGA 
TATAACTACA ACAACCTGGC CAGCATGCAT GCCAATGTCA TAGTTCGCCC CACACCGCGA 
CCCACTCCAG CCAGCTATGC CAAGTTCACA CTGCAGACAG TGAGCCTGCC CAGGCCGGAG 
TACCCGGTGG CCATCAGTTT GACAGCCACC ACACCAACCA CGCCCAGCAG CAGTGTGCAG 
TCACCTGTGC CCGCCCATTC GACAGGTGCC AGGGCCAAGG ACATCTCGAC CAGTTCCAGG 
CAACATCCTT TGACCTCGGT CCATGTCCAA CCACAGTCGC GTCATCTTGA CCAGAAGAGC 
GTGAAGCAGC TAACGAATAA CTCCACACGA CGAGGATTCT CCGGAAGCAG GGAGATTAGC 
GCCGATTCTG GGATAGCCAG CATGGACATG GCTTTGGACA GCAGTTCGGG CAGCTCGGTG 
GGTTCCAAGA GGAGTCGGAG CAGACCAAGG AACCTAAAGA TGGTGATGAG TGGACGGCAC 
ACATTCGAGG TGCGCGATGC GGATGATCCG CCATCCAGTG AGTCCAACTC CTTTGTGGAA 
CCACTGGCAC TACCAAAGTT ACCCACTGAT GGAAGTCAGA GTATTCCTCT GCCATTGCTG 
GGCTTGGTGC GATCCAATAC GGTGTTGAGT CGGGAGTCTT ACGAGCGACG TCAGGCGGAA 
ACACCAGGAT CCCAGGATCA GCAGGATTCG GAAAAACCCA AAACCAGCGG CTCCGATGAA 
AGCGAGAGTG TGGATGAAGA GAAACTCTAC TTGGACTCGT CCACCTCCGA AAAGAGTGCC 
AAACATCAGA GCCAATCGTC AGTGGCTTCC ACTTGGCGTC ACCAGGCGGG TGAATCTCTG 
GCCGCCCAGG ATTGCAGCAG CATGAGCATG AGCATCAGTA GTGACACCCA TGCTCCGGAA 
AATCCGCGCG ATAATGACAA GGAGGAGGAC ATGTCCTTGG GTCTGGATGA GATCAGTCTG 
ATCAACACTG ACATGCAGTT TAGCACAATC TCTTCAATGA CGGAAACTCC ACCCAAAGTG 
GGTCAGGAGT CCCTGCTCAA TCTTCACCTG GTGGACAATC GGGAGGCAGG CACCTCGCGT 
CCCCGCTCCT TTAACAATGC TCTTAATGAA TCCAAGTTTG CCGAGCTGGC TCTGGCCAGT 
AGTAGTTGCC TCCTGTTGGA TGATGAGACA TCGCCCACAG ACAGTTTGGT CAGCAGCACC 
GAAGATTCCG AGGAGGCTGG TGGCAAGTTG CAAAAGCACA AGCTCAATGA GGAGCGTCAG 
CAGAAGGACA TCGATGACAT CGACATAGAT GATATATCAC CGGTTCTCGA ACTGGATCTG 
GATCCTCCAG GTGGACGAAG TCCCATTTCA CCCGGCACTC CCACGCATGC ATCCCATTCC 
CTTTCTTTGG GTTCGGATTG CGGAAATCTT ATTGATGATG AGATCGCCGA TCAGCCAGCA 
TTGCTATGCA ACAGTGAAGC CCATGAGGTG GCCACCGATA CGCCCACATT GATGGAAACC 
CTCACGCACA CCCAAACAGG ATCCTTGAGA TCCTTGAAGA GTCAGTCGAA GGCACGTACC 
GCTCTGCAGC AGGCCATCGA GTTGAGTTTA AGGACTCCGG CAGCGGTACG AAAGGCAGTT 
ATGGATCGAG CCGAATCCTT GGATACTCTA TCGCCATGCG AATCCATTTG CTCCGATGAC 
CTGATGATGG ACTTCGATAT GAACAGCAGC GTGGACTCGA TCGATCACAT GGCCAGTTCA 
ACGGGACGCA GTCGCAGTGG CTCGGATCTT CACAAGATTG GTGGTCAGGA TATCGATCCC 
ATGCAGGCGG AAACTGAGGC GGAACTTCTA TCCGAGTTGG AACGCAGGGG CAGTGATGTG 
ATGAAGGAGC TCAATACTTT GCTGCGAGGA AGGAGGCAGC GCGGAGGACC AAGGGAGAGG 
ATTAGCGCCC AACTGCCTGC GCGTGCCACC AGATTGCTGA ACCGCTCACG TCTGCAGGAC 
CAACAACTCA CCGGCAACGA TTCGGATAAC AGCTTGAGGT CCTCCCACAG CGGCGGAGCT 
TCGGCGGCGG CCAGGAAGCG AAGCACGACC AACTCAAGGA CCTCGTCGGG TTCCACGGCC 
AGCCTGCCTC GTCAGCGTCA TCTGCAGCAG CAGCATCTGG GATCGGGTGG CGGAGCAGGA 
GCGGGTGGTG GAGGTGGAGG TGGTGGAGCC ACTGCGTCCG GTACATCCAC CCAGAGGTGC 
GGGGGAGAGC TGCACAGCTC GTCGGACGAT CTAATGTTGT ATGACAAGTC CTTCCGCAAT 
GCGATGATCC AGGATGTGCT GCAGTTCAAG AAGCAGCTGC TCCGACTGCG GCGAATACTC 
CAGGAGCCCC AAAAGGAGCC AGATTTTGAT TTGAAGCTGA CGGAAACGCT CAATCCCTTC 
GAGAACGATA ACGTCCAGCT GTTCGCCGCC TGCGGATTGG ATAGCAAGCA ATTGAATGAC 
ATCGATTTGG CCAGCCTAAC CTCGTCCACG ACGGAGGATC CGCTACAGGA GCTAACGGAT 
CTACGTAGAC AGGTGGTTTA CCTTCAGGGT CAAGTGGACG ATCGTGATCG CACCATTCGC 
CTGCAGCGCG ATCTCATCGA GCAACTGGAG GCCGAGAAGA GGCAGAAGGC TTCGCATGGA 
CCGGGATCTG GCGCGGATCC GGGCAAGGAG CTAATCAGCA TGGCCACCCA AACGGAGCGG 
ACACGACCAC TTGCCATTGG TGCGGAGGGT TTGTCCAGAA GTAAGCCCGA ATATACCAGT 
TATACCACGC ACTTTCCGAT GCTCCACTTG CACGATTCCA CGCTGGCAGC CACCACGATA 
ATCCGTCATC ATCAGAGCAA CCATGAGCAG CAACAGCCGG AGAAATCCTG TCCAGCTCTC 
AGCCAAACCC GCCGGCACAC CATTATTTCC ACCACGCTGA CCAACTACAA TCAGCAGCTG 
GCGGCCGCAT TTCCCGATGC TACGCGACGC TCCTCCATCG GTTGGGATAC CACTCCGACC 
ACGCCCACCC CGCATGGCAA CGCCTCCAAG CCGGTAAGGA TTACGCTAAT CGGCGAGGCA 
CAGCCGCTGC AGAGCAAGTC ATCAACCGGA TCGCTATGCT CGTCATCATC ATCGTCGTCG 
TCATCCTCCT CATCATCAAC ATCCTTGTTG GCACAAAATG CCAATGTGGT CAAGATGCGA 
TATACCAATG GCTGTCAGAG TGTTAAGAAC GGATCGAGTG CCCATTATCA GCCGTTGTAC 
AACAGCAACA AGATGACAAG CCCAACCGTA ACTATAGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG42268 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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