alphaTub67C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alphaTub67C
Entrez Gene ID	39130
Full Name	alpha-Tubulin at 67C
Synonyms	4t,ALPHA 67C,CG8308,D.m.ALPHA-67C,DTA2,Dmel\CG8308,Fs(3)Kav,Fs(3)Kavar,Fs(3)Sz14,Fs(3)Sz22,Fs(3)Tom,Fs(3)Tomaj,Kavar,T,Tom,Tub,Tub1E,aTub67C,alpha-Tub,alpha-Tub67C,alpha-tub,alpha-tub67C,alpha-tubulin,alpha-tubulin[4],alpha4,alpha4-t,alpha4-tubulin,alpha4Tub67C,alpha4t,alpha67C,alphaTUB,alphaTub,alphaTub 67C,alphaTub4,alphaTub[67C],alphatub,alphatub67C,gamma-Tub,mum-tub67C,tub,tub67c
General protein information	Preferred Names alpha-Tubulin at 67C Names CG8308 gene product from transcript CG8308-RA CG8308-PA alpha tubulin alpha tubulin 67C alpha-Tubulin alpha4-tubulin alphaT alphaTub67C-PA alphaTubulin 67C alphaTubulin67C alphatubulin female sterile (3) kavar kavar tomaj tubulin tubulin at 67C tubulin67C
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C4-67C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079285.4	NP_524009.2	alpha-Tubulin at 67C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007122	XP_308639.4
Drosophila melanogaster (fruit fly)	alphaTub67C	NP_524009.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glu426 is involved in stabilization of the microtubule-kinesin complexes when the kinesins are in the most labile, ADP-bound state.
Title: Glu415 in the alpha-tubulins plays a key role in stabilizing the microtubule-ADP-kinesin complexes.

Data show that alpha4-tubulin is enriched in the long microtubules that embrace the nuclear envelope and suggest that they push apart daughter centrosomes along the nuclear perimeter during the initial cleavage divisions.
Title: alpha4-Tubulin is involved in rapid formation of long microtubules to push apart the daughter centrosomes during earlyx Drosophila embryogenesis.

The Kavar(D) dominant female-sterile mutations reveal a role for the maternally provided alpha-tubulin4 isoform in cleavage spindle maintenance and elongation.
Title: The Kavar(D) dominant female-sterile mutations of Drosophila reveal a role for the maternally provided alpha-tubulin4 isoform in cleavage spindle maintenance and elongation.

The following alphaTub67C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alphaTub67C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18077	NM_079285.4 Latest version!	Drosophila melanogaster alpha-Tubulin at 67C (alphaTub67C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alphaTub67C ( NM_079285.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524009.2 Drosophila melanogaster alpha-Tubulin at 67C (alphaTub67C), mRNA.

Price & Availability↑

CloneID	OFa18077
Clone ID Related Accession (Same CDS sequence)	NM_079285.4
Accession Version	NM_079285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Tubulin at 67C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079285.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGAAG TAGTCTCCAT CCAGATTGGC CAGTGCGGCA TCCAGATCGG CAATGCCTGC 
TGGGAGCTGT ACCTGCTGGA GCACGGCATC AACCTGGACG GCAGCCTGAA GACCAAGGAG 
GAGCTGACGG CCAGCGGGAG CAGTGCCAGT GTGGGTCACG ACACCTCGGC CAACGATGCT 
AGGACCTTCT TCACGGAGAC CGGCAATGGA AAACAAGTGC CACGCTCGAT TTTCGTCGAT 
CTGGAACCGA CGGTCATCGA TGATGTTCGG AATGGCTGCA TGAGGGAGCT CTATCATCCG 
GAGCAACTGA TTTCTGGAAA GGAAGATGCG GCCAATAACT ATGCCCGAGG TCGTTACTCC 
ATTGGCAAGG AGGTGATCGA TAGGGTGACC TCACGGCTGC AGAAGATCGC AGAGCAGTGT 
GACAGCTTGC AGGGATTCCT CATCTTCCAC TCGCTGGGCG GTGGTACTGG TTCGGGATTC 
ACATCCCTGT TGGTGGAGCG CTTGTCCACC GATTACAGCA AGAAGTGCAA GCTGGACTTT 
GCCGTCTATC CATCGCCCAA GGTCTCCACC GCCGTGGTGG AGCCATATAA CGCGCTGCTC 
ACCACCCACT CCACCATGGA CCACTCGGAC TGTGTGTTCA TGGTGGACAA CGAGGCCATC 
TACGATATAT GTAACAATAG CTTGGGTGTG GACAGGCCCG CCTATAGGAA TCTGAATCGT 
TTGATCGCCC AAATAGTGAG CTCCACAACG GCTTCTCTGC GTTTCAGTGG CTCCATGAAT 
GTGGATCTGA ATGAGTTCCA GACGAATCTG GTACCCTTCC CCAGAATCCA CTTTCCCCTG 
GTTGCCTATG CCCCACTGAT GTCCGCCGAG AGATCGGCCC ATGAACAGCA TGCGATTACC 
ACATTGACCA ATGCCTGTTT CGAATCCTCC AACATGATGG TCAAGTGTGA TCCTCGTGCG 
GGCAAATTCA TGGCCTGTTG CATGCTTTAC AGGGGTGATG TGGTGCCCAA GGATGTAAAT 
GCCGCTGTCT CGGCCATCAA GTCCAAGCGG CACATTCAAT TCGTGGACTG GTGTCCCACT 
GGTTTCAAAA TTGGCATCAA CTACGAGAAG CCAGCCTTTG TGCCGGATGG AGATTTGGCC 
AAGACCTCGA GGGCCTGCTG CATGCTGTCC AACACCACCG CCATCTCGGT GGCCTTCTCC 
AATCTCTCCT ACAAGTTCGA TCTGATGTTC AAGAAGCGGG CCTTCGTCCA TTGGTACGTG 
GGCGAGGGCA TGGAGGAGGG CGAGTTCACG GAGGCGCGCG AGAATATCGC CGTACTGGAA 
CGGGACTTCG AGGAGGTCGG TCTGGACAAT GCCGAGGAGG GCGGCGACGA GGACTTCGAT 
GAGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524009.2
CDS	147..1535
Translation	MREVVSIQIGQCGIQIGNACWELYLLEHGINLDGSLKTKEELTASGSSASVGHDTSANDARTFFTETGNGKQVPRSIFVDLEPTVIDDVRNGCMRELYHPEQLISGKEDAANNYARGRYSIGKEVIDRVTSRLQKIAEQCDSLQGFLIFHSLGGGTGSGFTSLLVERLSTDYSKKCKLDFAVYPSPKVSTAVVEPYNALLTTHSTMDHSDCVFMVDNEAIYDICNNSLGVDRPAYRNLNRLIAQIVSSTTASLRFSGSMNVDLNEFQTNLVPFPRIHFPLVAYAPLMSAERSAHEQHAITTLTNACFESSNMMVKCDPRAGKFMACCMLYRGDVVPKDVNAAVSAIKSKRHIQFVDWCPTGFKIGINYEKPAFVPDGDLAKTSRACCMLSNTTAISVAFSNLSYKFDLMFKKRAFVHWYVGEGMEEGEFTEARENIAVLERDFEEVGLDNAEEGGDEDFDEF

Target ORF information:

RefSeq Version	NM_079285.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Tubulin at 67C (alphaTub67C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079285.4

ORF Insert Sequence:

ATGCGCGAAG TAGTCTCCAT CCAGATTGGC CAGTGCGGCA TCCAGATCGG CAATGCCTGC 
TGGGAGCTGT ACCTGCTGGA GCACGGCATC AACCTGGACG GCAGCCTGAA GACCAAGGAG 
GAGCTGACGG CCAGCGGGAG CAGTGCCAGT GTGGGTCACG ACACCTCGGC CAACGATGCT 
AGGACCTTCT TCACGGAGAC CGGCAATGGA AAACAAGTGC CACGCTCGAT TTTCGTCGAT 
CTGGAACCGA CGGTCATCGA TGATGTTCGG AATGGCTGCA TGAGGGAGCT CTATCATCCG 
GAGCAACTGA TTTCTGGAAA GGAAGATGCG GCCAATAACT ATGCCCGAGG TCGTTACTCC 
ATTGGCAAGG AGGTGATCGA TAGGGTGACC TCACGGCTGC AGAAGATCGC AGAGCAGTGT 
GACAGCTTGC AGGGATTCCT CATCTTCCAC TCGCTGGGCG GTGGTACTGG TTCGGGATTC 
ACATCCCTGT TGGTGGAGCG CTTGTCCACC GATTACAGCA AGAAGTGCAA GCTGGACTTT 
GCCGTCTATC CATCGCCCAA GGTCTCCACC GCCGTGGTGG AGCCATATAA CGCGCTGCTC 
ACCACCCACT CCACCATGGA CCACTCGGAC TGTGTGTTCA TGGTGGACAA CGAGGCCATC 
TACGATATAT GTAACAATAG CTTGGGTGTG GACAGGCCCG CCTATAGGAA TCTGAATCGT 
TTGATCGCCC AAATAGTGAG CTCCACAACG GCTTCTCTGC GTTTCAGTGG CTCCATGAAT 
GTGGATCTGA ATGAGTTCCA GACGAATCTG GTACCCTTCC CCAGAATCCA CTTTCCCCTG 
GTTGCCTATG CCCCACTGAT GTCCGCCGAG AGATCGGCCC ATGAACAGCA TGCGATTACC 
ACATTGACCA ATGCCTGTTT CGAATCCTCC AACATGATGG TCAAGTGTGA TCCTCGTGCG 
GGCAAATTCA TGGCCTGTTG CATGCTTTAC AGGGGTGATG TGGTGCCCAA GGATGTAAAT 
GCCGCTGTCT CGGCCATCAA GTCCAAGCGG CACATTCAAT TCGTGGACTG GTGTCCCACT 
GGTTTCAAAA TTGGCATCAA CTACGAGAAG CCAGCCTTTG TGCCGGATGG AGATTTGGCC 
AAGACCTCGA GGGCCTGCTG CATGCTGTCC AACACCACCG CCATCTCGGT GGCCTTCTCC 
AATCTCTCCT ACAAGTTCGA TCTGATGTTC AAGAAGCGGG CCTTCGTCCA TTGGTACGTG 
GGCGAGGGCA TGGAGGAGGG CGAGTTCACG GAGGCGCGCG AGAATATCGC CGTACTGGAA 
CGGGACTTCG AGGAGGTCGG TCTGGACAAT GCCGAGGAGG GCGGCGACGA GGACTTCGAT 
GAGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alphaTub67C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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