nwk cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following nwk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nwk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa20776 NM_001259763.2
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa20778 NM_001259761.2
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa21999 NM_001300067.1
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OFa22000 NM_001300065.1
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OFa22001 NM_001300066.1
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OFa22002 NM_001300068.1
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OFa22003 NM_001300064.1
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
OFa17996 NM_001259762.2
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa17996 NM_140033.3
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa20777 NM_001259760.2
Latest version!
Drosophila melanogaster nervous wreck (nwk), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa20776
    Clone ID Related Accession (Same CDS sequence) NM_001259763.2
    Accession Version NM_001259763.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259763.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGGCC AGAACCAAAG CCAAACCACT
    GCCAAGAAGG GTAATTTAAT GAATATGCTC TTTTATCTAA ACTATCTTAT ACCCGAAACT
    TTTCCTGATT CAGTAATTCT TAGTGTAGGA AAATCTATTT CAACTCCACT GTTTGAGAGC
    ATGTCCAATC CCCCTCTTAA CTATTGTTCA ACTTTCAAAA TGAGATTTTC TTCTTGGTAT
    ATAGAAAAAA ATATACAATA TATATTCAGT ATAAGGTTTT TATCAACCTG TTTACAACTA
    TTCCCTAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246692.1
    CDS126..2783
    Translation

    Target ORF information:

    RefSeq Version NM_001259763.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259763.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGGCC AGAACCAAAG CCAAACCACT
    GCCAAGAAGG GTAATTTAAT GAATATGCTC TTTTATCTAA ACTATCTTAT ACCCGAAACT
    TTTCCTGATT CAGTAATTCT TAGTGTAGGA AAATCTATTT CAACTCCACT GTTTGAGAGC
    ATGTCCAATC CCCCTCTTAA CTATTGTTCA ACTTTCAAAA TGAGATTTTC TTCTTGGTAT
    ATAGAAAAAA ATATACAATA TATATTCAGT ATAAGGTTTT TATCAACCTG TTTACAACTA
    TTCCCTAAAG ACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20778
    Clone ID Related Accession (Same CDS sequence) NM_001259761.2
    Accession Version NM_001259761.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259761.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGGTA AATGTCATAT CCTCGCCCTA
    TCAACTGGTC TTTTGTTTCC CCTATCTATA TCTCTCTTTA AACTATATCC ATATCGATTC
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246690.1
    CDS126..2591
    Translation

    Target ORF information:

    RefSeq Version NM_001259761.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259761.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGGTA AATGTCATAT CCTCGCCCTA
    TCAACTGGTC TTTTGTTTCC CCTATCTATA TCTCTCTTTA AACTATATCC ATATCGATTC
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21999
    Clone ID Related Accession (Same CDS sequence) NM_001300067.1
    Accession Version NM_001300067.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG TACTCATCCA AGAGCCTGGC
    ATGGAGGACG ATCTCAGTGA CGATGGGCAG CCGCCGCCGT CCTTGCCGCC GCCCCAACTA
    GCGAAGGCGG GCGGATCCGC TCCTGGATCT GGGAGCAAGG TCGAAAAGGG GGCGGCGGCA
    GGTGGCGCCA ACACGCTGAA CTTAGGTATG GCACAAATAA TTGTTACCGC TGCAACCCCC
    ATGGTTGAAG ACGGTGCCGA TAAATCTTTC CCACCAGTAG GCGAGAGCGA TGCACAGCCA
    GTGGAGCCGG TGTCCAAGGA GCAGCCGGCG GAAGTGGCCA AAAAGCCAGA TATTGCGCCC
    AAGCCGCTGG CCAAGGTGGC GCCACAGAGC GCGCCGGCCA AAGAAGGTAA TGCTGGAGTG
    AGGCCCGTGG TGAGCATTAC ACTGACCGAG TATCCATCCT GTGATGCAGA GGACCAACAG
    TCCTTCTCGG AGGGCACCGA TTCGGCTTCG GTTGCCGATG TGCCGGTACT GCAGGATGCG
    GAGGATCCAT TCAACGAGAA GGCCAAGGGA GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC
    AATTTTGAGG CCAACTTCGA TGCCAATTTT GATGACGCCT TCGCTGGAAT CGGAGGAGGT
    GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG TCCAACGAAT TGGACATCAA CGGAGAGGCA
    GCAGGAGAAG CAATAGTATC TGGATCTGCG GCTGGCGATG AGGATATTGA GGCACCCAAA
    CAGGTGGTCG GTGGGCGAGC TAGCATACCC GAGGAATTGG ACTCAAATCA ATTGGCACGT
    TTGCAAAATC TGAAGGAGTC GAACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286996.1
    CDS201..3350
    Translation

    Target ORF information:

    RefSeq Version NM_001300067.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300067.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG TACTCATCCA AGAGCCTGGC
    ATGGAGGACG ATCTCAGTGA CGATGGGCAG CCGCCGCCGT CCTTGCCGCC GCCCCAACTA
    GCGAAGGCGG GCGGATCCGC TCCTGGATCT GGGAGCAAGG TCGAAAAGGG GGCGGCGGCA
    GGTGGCGCCA ACACGCTGAA CTTAGGTATG GCACAAATAA TTGTTACCGC TGCAACCCCC
    ATGGTTGAAG ACGGTGCCGA TAAATCTTTC CCACCAGTAG GCGAGAGCGA TGCACAGCCA
    GTGGAGCCGG TGTCCAAGGA GCAGCCGGCG GAAGTGGCCA AAAAGCCAGA TATTGCGCCC
    AAGCCGCTGG CCAAGGTGGC GCCACAGAGC GCGCCGGCCA AAGAAGGTAA TGCTGGAGTG
    AGGCCCGTGG TGAGCATTAC ACTGACCGAG TATCCATCCT GTGATGCAGA GGACCAACAG
    TCCTTCTCGG AGGGCACCGA TTCGGCTTCG GTTGCCGATG TGCCGGTACT GCAGGATGCG
    GAGGATCCAT TCAACGAGAA GGCCAAGGGA GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC
    AATTTTGAGG CCAACTTCGA TGCCAATTTT GATGACGCCT TCGCTGGAAT CGGAGGAGGT
    GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG TCCAACGAAT TGGACATCAA CGGAGAGGCA
    GCAGGAGAAG CAATAGTATC TGGATCTGCG GCTGGCGATG AGGATATTGA GGCACCCAAA
    CAGGTGGTCG GTGGGCGAGC TAGCATACCC GAGGAATTGG ACTCAAATCA ATTGGCACGT
    TTGCAAAATC TGAAGGAGTC GAACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22000
    Clone ID Related Accession (Same CDS sequence) NM_001300065.1
    Accession Version NM_001300065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACACTAC
    CATGAGCATG AAATATACTA TGTAGACTAT AGCCACGGAC AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286994.1
    CDS126..3353
    Translation

    Target ORF information:

    RefSeq Version NM_001300065.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACACTAC
    CATGAGCATG AAATATACTA TGTAGACTAT AGCCACGGAC AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22001
    Clone ID Related Accession (Same CDS sequence) NM_001300066.1
    Accession Version NM_001300066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG TACTCATCCA AGAGCCTGGC
    ATGGAGGACG ATCTCAGTGA CGATGGGCAG CCGCCGCCGT CCTTGCCGCC GCCCCAACTA
    GCGAAGGCGG GCGGATCCGC TCCTGGATCT GGGAGCAAGG TCGAAAAGGG GGCGGCGGCA
    GGTGGCGCCA ACACGCTGAA CTTAGGTATG GCACAAATAA TTGTTACCGC TGCAACCCCC
    ATGGTTGAAG ACGGTGCCGA TAAATCTTTC CCACCAGTAG GCGAGAGCGA TGCACAGCCA
    GTGGAGCCGG TGTCCAAGGA GCAGCCGGCG GAAGTGGCCA AAAAGCCAGA TATTGCGCCC
    AAGCCGCTGG CCAAGGTGGC GCCACAGAGC GCGCCGGCCA AAGAAGGTAA TGCTGGAGTG
    AGGCCCGTGG TGAGCATTAC ACTGACCGAG TATCCATCCT GTGATGCAGA GGACCAACAG
    TCCTTCTCGG AGGGCACCGA TTCGGCTTCG GTTGCCGATG TGCCGGTACT GCAGGATGCG
    GAGGATCCAT TCAACGAGAA GGCCAAGGGA GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC
    AATTTTGAGG CCAACTTCGA TGCCAATTTT GATGACGCCT TCGCTGGAAT CGGAGGAGGT
    GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG TCCAACGAAT TGGACATCAA CGGAGAGGCA
    GCAGGAGAAG CAATAGTATC TGGATCTGCG GCTGGCGATG AGGATATTGA GGCACCCAAA
    CAGGTGGTCG GTGGGCGAGC TAGCATACCC GAGGAATTGG ACTCAAATCA ATTGGCACAC
    TACCATGAGC ATGAAATATA CTATGTAGAC TATAGCCACG GACAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286995.1
    CDS126..3296
    Translation

    Target ORF information:

    RefSeq Version NM_001300066.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG TACTCATCCA AGAGCCTGGC
    ATGGAGGACG ATCTCAGTGA CGATGGGCAG CCGCCGCCGT CCTTGCCGCC GCCCCAACTA
    GCGAAGGCGG GCGGATCCGC TCCTGGATCT GGGAGCAAGG TCGAAAAGGG GGCGGCGGCA
    GGTGGCGCCA ACACGCTGAA CTTAGGTATG GCACAAATAA TTGTTACCGC TGCAACCCCC
    ATGGTTGAAG ACGGTGCCGA TAAATCTTTC CCACCAGTAG GCGAGAGCGA TGCACAGCCA
    GTGGAGCCGG TGTCCAAGGA GCAGCCGGCG GAAGTGGCCA AAAAGCCAGA TATTGCGCCC
    AAGCCGCTGG CCAAGGTGGC GCCACAGAGC GCGCCGGCCA AAGAAGGTAA TGCTGGAGTG
    AGGCCCGTGG TGAGCATTAC ACTGACCGAG TATCCATCCT GTGATGCAGA GGACCAACAG
    TCCTTCTCGG AGGGCACCGA TTCGGCTTCG GTTGCCGATG TGCCGGTACT GCAGGATGCG
    GAGGATCCAT TCAACGAGAA GGCCAAGGGA GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC
    AATTTTGAGG CCAACTTCGA TGCCAATTTT GATGACGCCT TCGCTGGAAT CGGAGGAGGT
    GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG TCCAACGAAT TGGACATCAA CGGAGAGGCA
    GCAGGAGAAG CAATAGTATC TGGATCTGCG GCTGGCGATG AGGATATTGA GGCACCCAAA
    CAGGTGGTCG GTGGGCGAGC TAGCATACCC GAGGAATTGG ACTCAAATCA ATTGGCACAC
    TACCATGAGC ATGAAATATA CTATGTAGAC TATAGCCACG GACAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22002
    Clone ID Related Accession (Same CDS sequence) NM_001300068.1
    Accession Version NM_001300068.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGGTATGGC ACAAATAATT GTTACCGCTG CAACCCCCAT GGTTGAAGAC
    GGTGCCGATA AATCTTTCCC ACCAGTAGGC GAGAGCGATG CACAGCCAGT GGAGCCGGTG
    TCCAAGGAGC AGCCGGCGGA AGTGGCCAAA AAGCCAGATA TTGCGCCCAA GCCGCTGGCC
    AAGGTGGCGC CACAGAGCGC GCCGGCCAAA GAAGAGGACC AACAGTCCTT CTCGGAGGGC
    ACCGATTCGG CTTCGGTTGC CGATGTGCCG GTACTGCAGG ATGCGGAGGA TCCATTCAAC
    GAGAAGGCCA AGGGAGAGTC CGGCGACGGT TCGGGATTTG AGGCCAATTT TGAGGCCAAC
    TTCGATGCCA ATTTTGATGA CGCCTTCGCT GGAATCGGAG GAGGTGGGGG CGGTGGAGGT
    GGCGGTGGGG AGCAGTCCAA CGAATTGGAC ATCAACGGAG AGGCAGCAGG AGAAGCAATA
    GTATCTGGAT CTGCGGCTGG CGATGAGGAT ATTGAGGCAC CCAAACAGGT GGTCGGTGGG
    CGAGCTAGCA TACCCGAGGA ATTGGACTCA AATCAATTGG CACACTACCA TGAGCATGAA
    ATATACTATG TAGACTATAG CCACGGACAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286997.1
    CDS126..3221
    Translation

    Target ORF information:

    RefSeq Version NM_001300068.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300068.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGGTATGGC ACAAATAATT GTTACCGCTG CAACCCCCAT GGTTGAAGAC
    GGTGCCGATA AATCTTTCCC ACCAGTAGGC GAGAGCGATG CACAGCCAGT GGAGCCGGTG
    TCCAAGGAGC AGCCGGCGGA AGTGGCCAAA AAGCCAGATA TTGCGCCCAA GCCGCTGGCC
    AAGGTGGCGC CACAGAGCGC GCCGGCCAAA GAAGAGGACC AACAGTCCTT CTCGGAGGGC
    ACCGATTCGG CTTCGGTTGC CGATGTGCCG GTACTGCAGG ATGCGGAGGA TCCATTCAAC
    GAGAAGGCCA AGGGAGAGTC CGGCGACGGT TCGGGATTTG AGGCCAATTT TGAGGCCAAC
    TTCGATGCCA ATTTTGATGA CGCCTTCGCT GGAATCGGAG GAGGTGGGGG CGGTGGAGGT
    GGCGGTGGGG AGCAGTCCAA CGAATTGGAC ATCAACGGAG AGGCAGCAGG AGAAGCAATA
    GTATCTGGAT CTGCGGCTGG CGATGAGGAT ATTGAGGCAC CCAAACAGGT GGTCGGTGGG
    CGAGCTAGCA TACCCGAGGA ATTGGACTCA AATCAATTGG CACACTACCA TGAGCATGAA
    ATATACTATG TAGACTATAG CCACGGACAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22003
    Clone ID Related Accession (Same CDS sequence) NM_001300064.1
    Accession Version NM_001300064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGGCGAGAG CGATGCACAG CCAGTGGAGC CGGTGTCCAA GGAGCAGCCG
    GCGGAAGTGG CCAAAAAGCC AGATATTGCG CCCAAGCCGC TGGCCAAGGT GGCGCCACAG
    AGCGCGCCGG CCAAAGAAGA GGACCAACAG TCCTTCTCGG AGGGCACCGA TTCGGCTTCG
    GTTGCCGATG TGCCGGTACT GCAGGATGCG GAGGATCCAT TCAACGAGAA GGCCAAGGGA
    GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC AATTTTGAGG CCAACTTCGA TGCCAATTTT
    GATGACGCCT TCGCTGGAAT CGGAGGAGGT GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG
    TCCAACGAAT TGGACATCAA CGGAGAGGCA GCAGGAGAAG CAATAGTATC TGGATCTGCG
    GCTGGCGATG AGGATATTGA GGCACCCAAA CAGGTGGTCG GTGGGCGAGC TAGCATACCC
    GAGGAATTGG ACTCAAATCA ATTGGCACAC TACCATGAGC ATGAAATATA CTATGTAGAC
    TATAGCCACG GACAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286993.1
    CDS126..3146
    Translation

    Target ORF information:

    RefSeq Version NM_001300064.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGGCGAGAG CGATGCACAG CCAGTGGAGC CGGTGTCCAA GGAGCAGCCG
    GCGGAAGTGG CCAAAAAGCC AGATATTGCG CCCAAGCCGC TGGCCAAGGT GGCGCCACAG
    AGCGCGCCGG CCAAAGAAGA GGACCAACAG TCCTTCTCGG AGGGCACCGA TTCGGCTTCG
    GTTGCCGATG TGCCGGTACT GCAGGATGCG GAGGATCCAT TCAACGAGAA GGCCAAGGGA
    GAGTCCGGCG ACGGTTCGGG ATTTGAGGCC AATTTTGAGG CCAACTTCGA TGCCAATTTT
    GATGACGCCT TCGCTGGAAT CGGAGGAGGT GGGGGCGGTG GAGGTGGCGG TGGGGAGCAG
    TCCAACGAAT TGGACATCAA CGGAGAGGCA GCAGGAGAAG CAATAGTATC TGGATCTGCG
    GCTGGCGATG AGGATATTGA GGCACCCAAA CAGGTGGTCG GTGGGCGAGC TAGCATACCC
    GAGGAATTGG ACTCAAATCA ATTGGCACAC TACCATGAGC ATGAAATATA CTATGTAGAC
    TATAGCCACG GACAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17996
    Clone ID Related Accession (Same CDS sequence) NM_001259762.2 , NM_140033.3
    Accession Version NM_001259762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259762.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACGTTTG
    CAAAATCTGA AGGAGTCGAA CGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246691.1
    CDS201..3407
    Translation

    Target ORF information:

    RefSeq Version NM_001259762.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACGTTTG
    CAAAATCTGA AGGAGTCGAA CGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17996
    Clone ID Related Accession (Same CDS sequence) NM_001259762.2 , NM_140033.3
    Accession Version NM_140033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140033.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACGTTTG
    CAAAATCTGA AGGAGTCGAA CGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_648290.3
    CDS126..3332
    Translation

    Target ORF information:

    RefSeq Version NM_140033.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_140033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GGTACTTCCG GCAATCAATT GCGATCTCAA
    ATCTCATTCT CATCTGTCGA TTATACCGTT GACAATGAAG ATCAGACAGT GGACTCGATG
    CAATCACCCG ACCAGGTGTC GGTCATCATG GCGCCCCAGA AGCGAGTCAA ATCGGACGTG
    GAATGGTGCA TCGCGCTGTA CGACTACGAC GCCACCGCCG AGGATGAGCT GACCTTCGAG
    GAGGGCGACA AGATCAAGAT CATCACGAAG ACCGCCCACG GCGTGGACGA CGGTTGGTGG
    GAGGGCGAAC TGGATGGCAA GTTCGGTAAC TTTCCATCGC TGGTCGTCGA GGAGTGCGAC
    GAGATGGGCG AGCCGCTTAG CGAGGGTGGC GACGAGTCAC CTCCGCCCAC CGCAGCGCCC
    ACTTTTGCAC TGCCACCCGC TCCGGCCCTG CCACCAGAGT ACGCCCACGA ACTGGAACTG
    GAGCTCACTG AGGATATGTT TGGTTCTCAG GATACAGCAG ATGAGGATAG CGGCTATATA
    CCCAACGGCG CTGCTGCCCC GAGTATTCCT CCGCCAGTAC TCATCCAAGA GCCTGGCATG
    GAGGACGATC TCAGTGACGA TGGGCAGCCG CCGCCGTCCT TGCCGCCGCC CCAACTAGCG
    AAGGCGGGCG GATCCGCTCC TGGATCTGGG AGCAAGGTCG AAAAGGGGGC GGCGGCAGGT
    GGCGCCAACA CGCTGAACTT AGGTATGGCA CAAATAATTG TTACCGCTGC AACCCCCATG
    GTTGAAGACG GTGCCGATAA ATCTTTCCCA CCAGTAGGCG AGAGCGATGC ACAGCCAGTG
    GAGCCGGTGT CCAAGGAGCA GCCGGCGGAA GTGGCCAAAA AGCCAGATAT TGCGCCCAAG
    CCGCTGGCCA AGGTGGCGCC ACAGAGCGCG CCGGCCAAAG AAGGTAATGC TGGAGTGAGG
    CCCGTGGTGA GCATTACACT GACCGAGTAT CCATCCTGTG ATGCAGAGGA CCAACAGTCC
    TTCTCGGAGG GCACCGATTC GGCTTCGGTT GCCGATGTGC CGGTACTGCA GGATGCGGAG
    GATCCATTCA ACGAGAAGGC CAAGGGAGAG TCCGGCGACG GTTCGGGATT TGAGGCCAAT
    TTTGAGGCCA ACTTCGATGC CAATTTTGAT GACGCCTTCG CTGGAATCGG AGGAGGTGGG
    GGCGGTGGAG GTGGCGGTGG GGAGCAGTCC AACGAATTGG ACATCAACGG AGAGGCAGCA
    GGAGAAGCAA TAGTATCTGG ATCTGCGGCT GGCGATGAGG ATATTGAGGC ACCCAAACAG
    GTGGTCGGTG GGCGAGCTAG CATACCCGAG GAATTGGACT CAAATCAATT GGCACGTTTG
    CAAAATCTGA AGGAGTCGAA CGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20777
    Clone ID Related Accession (Same CDS sequence) NM_001259760.2
    Accession Version NM_001259760.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nervous wreck, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259760.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGTAGGCGA GAGCGATGCA CAGCCAGTGG AGCCGGTGTC CAAGGAGCAG
    CCGGCGGAAG TGGCCAAAAA GCCAGATATT GCGCCCAAGC CGCTGGCCAA GGTGGCGCCA
    CAGAGCGCGC CGGCCAAAGA AGAGGACCAA CAGTCCTTCT CGGAGGGCAC CGATTCGGCT
    TCGGTTGCCG ATGTGCCGGT ACTGCAGGAT GCGGAGGATC CATTCAACGA GAAGGCCAAG
    GGAGAGTCCG GCGACGGTTC GGGATTTGAG GCCAATTTTG AGGCCAACTT CGATGCCAAT
    TTTGATGACG CCTTCGCTGG AATCGGAGGA GGTGGGGGCG GTGGAGGTGG CGGTGGGGAG
    CAGTCCAACG AATTGGACAT CAACGGAGAG GCAGCAGGAG AAGCAATAGT ATCTGGATCT
    GCGGCTGGCG ATGAGGATAT TGAGGCACCC AAACAGGTGG TCGGTGGGCG AGCTAGCATA
    CCCGAGGAAT TGGACTCAAA TCAATTGGCA CGTTTGCAAA ATCTGAAGGA GTCGAACGCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246689.1
    CDS126..3128
    Translation

    Target ORF information:

    RefSeq Version NM_001259760.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nervous wreck (nwk), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259760.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCCGC CGCCGCGTAA GGGAAACTAT GTGAAATTCC TCAAGAATTT GCACACGGAG 
    CAGGTGGCCA AGCTGCAGCT GAAGAATCAG CATGAATGCG ACCTCCTGGA GGACATCCGG
    CAGTTCACCA TCAAGCGTTC CGCTGTCGAG AAGTCATACA GTGAGTCGCT GCTGAAGATC
    TCATCGCAGT ATCTCAACAA GAAGATACCC AATATACCAG ATATTAAGAT GGAGGGCATG
    GAGGAACGCT GGAACATGTG GAGCGTTTGG CGCACTGTGC TCGAGGAGAA TGAGAAACTG
    GCTCGTGCTC GATTGGCCGC CATAGAGGTG TTTCAACAGC AAATCGCGGA TGAGGCCAAG
    GTTCTGCGGG ACTATAAGTT GGCCATAGCT AAGCGTTCGC TAGCCGGAAT TGTCAATGTG
    CAGAAGGAAC TCCATCTGAG CGTGGGCGAT GTGGATAAGA CCAAGAAATC ATACTTTGAC
    GAAGAGCATT GTGCTCACGA TGTTCGGGAC AAGGCCCGCG ATATCGAGGA GAAGCTTAAG
    AAGAAGAAGG GCTCCTTCTT TCAATCAATC ACTTCGCTGC AGAAGAACAG CGCCCGGGTC
    ACATCTCGCA AGGAGCTGCT GGAGGAGAAG TCATCTGGCG CCCGGAATGA CTACGTACTC
    AGCTTGGCGG CGGCCAATGC GCATCAGAAT CGCTACTTCA CCGTCGATCT GCAGACAACG
    ATGACCACCA TGGAGAACTA TGTTTTCGAG CGGGTTGCCG AGTACCTGAT GTTAATGGGA
    CGCACAGAGC TGTTGACTTG CTCGGCTACG CAGAATAGCT TCGGAAAGAT CCGTGACCAG
    GCGCAGCAGT TGACGCGGGA GTACAACCTG CAGTGCTGCT ACCTATTCTA TCCGGTGCTG
    AAGCAGCACA TCCAATATGA CTTCGAGGCG TGCGACAATG ATCCGGTGCG CAAGGTGACC
    GCGGAGCATG AGTCCGCCGC CGAGACGCTA ACCAAGGAGG CCAAGAATCT GGCCGGAAGA
    GTGGTCAAGG AGAATGCCTC GATCAGGGAG AATGCCAAGA AGTTGGCCTT GTGCCAGTCG
    CTGCGAGACT CTGGCCAGCG CACGGATCCC AACGATCCAA ATGGACCGGA TCTGGACACC
    AAGATCGAGG AGTTCCGTGA TCAAATCCGT CGATCCGAGA CGGAAAAGAC AAAAGCGGAG
    GCATGTCTGC AGTGCCTGCG CGATGGCGGC ATTAACGTGG ACGAGTGGGT GCAGGAGGCC
    GAGAATATGG GTGTACAGGA GCTGACGCGT TCGGCCAGTT CCATTTCGAT GCGCACCGAC
    GCCTCTGGCC AGGGCGAGAA TCCCAGTTCC GATTCATTCT ACGACAGCGA CAAGGAGGAG
    ACTCAGGCAG CTGCCCAAAC GAAGCCCAAG CAGGAGCAAC AGCTATCCAG GGATCGTACC
    TTCAGCGATA GTGAGGATGA GCCCGAAGTG CGTCCATCGG CAGCGGCAGC TTCTTCTGCA
    GCTGCTGCCT CGTCATCCAT GATGGCCAGT AGCGCTGGTG GCTGGGATGA TCCCACTGAG
    GTCAACTGGG GAGCTGGCGA GGAGGAGGAC GACAAGGACG AACCGATTGT TCCAGAACCC
    AAGGAGGCGA TCTTCAAGTG CACTGCACTC TACAGCTATA CGGCCCAGAA TCCCGACGAG
    CTCACCATCG TCGAAAATGA ACAGCTCGAG GTGGTTGGCG AAGGCGACGG CGATGGGTGG
    CTAAGGGCCC GCAACTATCG CGGCGAGGAG GGCTACGTGC CACACAACTA TCTGGACATC
    GATCAGGAGA CAGCGGGCAG CGCCTTTAAT GATCAGACAG TGGACTCGAT GCAATCACCC
    GACCAGGTGT CGGTCATCAT GGCGCCCCAG AAGCGAGTCA AATCGGACGT GGAATGGTGC
    ATCGCGCTGT ACGACTACGA CGCCACCGCC GAGGATGAGC TGACCTTCGA GGAGGGCGAC
    AAGATCAAGA TCATCACGAA GACCGCCCAC GGCGTGGACG ACGGTTGGTG GGAGGGCGAA
    CTGGATGGCA AGTTCGGTAA CTTTCCATCG CTGGTCGTCG AGGAGTGCGA CGAGATGGGC
    GAGCCGCTTA GCGAGGGTGG CGACGAGTCA CCTCCGCCCA CCGCAGCGCC CACTTTTGCA
    CTGCCACCCG CTCCGGCCCT GCCACCAGAG TACGCCCACG AACTGGAACT GGAGCTCACT
    GAGGATATGT TTGGTTCTCA GGATACAGCA GATGAGGATA GCGGCTATAT ACCCAACGGC
    GCTGCTGCCC CGAGTATTCC TCCGCCAGTA CTCATCCAAG AGCCTGGCAT GGAGGACGAT
    CTCAGTGACG ATGGGCAGCC GCCGCCGTCC TTGCCGCCGC CCCAACTAGC GAAGGCGGGC
    GGATCCGCTC CTGGATCTGG GAGCAAGGTC GAAAAGGGGG CGGCGGCAGG TGGCGCCAAC
    ACGCTGAACT TAGTAGGCGA GAGCGATGCA CAGCCAGTGG AGCCGGTGTC CAAGGAGCAG
    CCGGCGGAAG TGGCCAAAAA GCCAGATATT GCGCCCAAGC CGCTGGCCAA GGTGGCGCCA
    CAGAGCGCGC CGGCCAAAGA AGAGGACCAA CAGTCCTTCT CGGAGGGCAC CGATTCGGCT
    TCGGTTGCCG ATGTGCCGGT ACTGCAGGAT GCGGAGGATC CATTCAACGA GAAGGCCAAG
    GGAGAGTCCG GCGACGGTTC GGGATTTGAG GCCAATTTTG AGGCCAACTT CGATGCCAAT
    TTTGATGACG CCTTCGCTGG AATCGGAGGA GGTGGGGGCG GTGGAGGTGG CGGTGGGGAG
    CAGTCCAACG AATTGGACAT CAACGGAGAG GCAGCAGGAG AAGCAATAGT ATCTGGATCT
    GCGGCTGGCG ATGAGGATAT TGAGGCACCC AAACAGGTGG TCGGTGGGCG AGCTAGCATA
    CCCGAGGAAT TGGACTCAAA TCAATTGGCA CGTTTGCAAA ATCTGAAGGA GTCGAACGCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.