GNBP3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNBP3
Entrez Gene ID	39020
Full Name	Gram-negative bacteria binding protein 3
Synonyms	CG5008,DGNBP-3,Dmel\CG5008,GNBP,GNBP-3,Gnbp3,Hades,gnbp3
General protein information	Preferred Names Gram-negative bacteria binding protein 3 Names CG5008 gene product from transcript CG5008-RA CG5008-PA GNBP3-PA Gram-negative binding protein 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E5-66E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079262.3	NP_523986.2	Gram-negative bacteria binding protein 3

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	GNBP3	NP_523986.2
Anopheles gambiae (African malaria mosquito)	GNBPA2	XP_320146.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Spn1 is an active protease inhibitor of the serpin superfamily, and support a role of Spn1 as a repressor of GNBP3-dependent Toll activation upon fungal infection.
Title: Spn1 regulates the GNBP3-dependent Toll signaling pathway in Drosophila melanogaster.

GNBP3 agglutinates fungal cells & activates melanization in a Toll-independent manner. GNBP3 assembles "attack complexes", which comprise phenoloxidase and the necrotic serpin.
Title: The Drosophila PRR GNBP3 assembles effector complexes involved in antifungal defenses independently of its Toll-pathway activation function.

N-terminal domain of Drosophila Gram-negative binding protein 3 (GNBP3) has a role in beta-glucan recognition
Title: The N-terminal domain of Drosophila Gram-negative binding protein 3 (GNBP3) defines a novel family of fungal pattern recognition receptors.

GNBP3-N terminal crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 134.79, b = 30.55, c = 51.73 A, beta = 107.4 degrees, and diffracted to 1.7 A using synchrotron radiation.
Title: Expression, purification, crystallization and preliminary X-ray analysis of the N-terminal domain of GNBP3 from Drosophila melanogaster.

Here, we report that GNBP3 is a pattern recognition receptor that is required for the detection of fungal cell wall components. Strikingly, we find that there is a second, parallel pathway acting jointly with GNBP3.
Title: Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors.

The following GNBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17960	NM_079262.3 Latest version!	Drosophila melanogaster Gram-negative bacteria binding protein 3 (GNBP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNBP3 ( NM_079262.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523986.2 Drosophila melanogaster Gram-negative bacteria binding protein 3 (GNBP3), mRNA.

Price & Availability↑

CloneID	OFa17960
Clone ID Related Accession (Same CDS sequence)	NM_079262.3
Accession Version	NM_079262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gram-negative bacteria binding protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_079262.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGATG CATTGCGCTT TGTGGCCTGG AGCTGCTGCC TCCAACTGCT GTTCCTGCTC 
CTTGGCGTTC AGGGGTATGA AGTACCCAAG GCTAAGATCG ATGTTTTCTA TCCCAAGGGA 
TTCGAGGTCT CAATTCCCGA TGAGGAGGGC ATCACCCTGT TCGCCTTCCA TGGCAAACTA 
AATGAGGAGA TGGAGGGCCT GGAGGCTGGC ACCTGGGCAC GGGACATTGT CAAAGCGAAA 
AACGGACGCT GGACCTTCCG GGACCGGATT ACGGCTCTAA AGCCTGGCGA CACCCTGTAC 
TACTGGACAT ACGTTATCTA CAACGGACTG GGTTATCGCG AAGACGACGG GTCGTTTGTG 
GTCAATGGGT ACAGTGGTAA TAATGCCTCA CCACATCCAC CTGTGGTTCC AGTCTCCACA 
ACGCCCTGGA CTCCACCTGC CGATCCGGAT ATCGACATAA GGTTGGGTTG TACTACTCCC 
AAAACCGAGG TCAATGGAGC ACCCACTCGC TGTGCCGGGC AGCTGGTGTT CGTGGATGAG 
TTCAATGCTG CAAAGCTGGA TCCCAATAAG TGGAAAGCGG AACGCAGGTT TTCCGGTCAA 
CCCGACTACG AGTTTAATGT GTACGTGGAT GACGCTCCGG AAACCTTGTG CTTAGCCAAT 
GGTCATGTGG TGCTCTCGAC GAACACAATG AAAAAACAAT TCAAGAAGGG TTCGGGTGAA 
AGTTTGGATC TGGGTGAAAA GTGTACGGGA CAAGCGAATA CCCACGACTG TGTAAGAAAT 
GGGCGAACCC TAAATGACGG ACTTCCGCCA ATGGTCACCG CTCAGTTCTC CTCCAAGGAT 
TTCTCCTTCA AGTATGGTCG CGTGGAGGTG CGAGCCAAAA TGCCGCGGGC ACAGTGGGTG 
ACTCCACAAA TCTGGCTGCA GCCCAGGCGT CCGATCTACG GAGTGGATGA TTATCGGTCG 
GGACAGCTGA GAATCGCTTA TACCCGTCCG AATGGAGGTA ATCTGGATTT GTATGGCGCC 
GCCGTGCTCT TTGCAGATGA GCCGCTGCGA TCGGTCAAGA ATTGCCTGAA GCCAGGTACT 
GGAAATAACT CCGAGGACTG GAGCGATAGT TTCCACAATT ACACCCTCGA ATGGACACCC 
AGGGAACTTC GCTGGCTGGT GGACGGCAAG GAGTGGTGTG TCCAGGGAAG TGCTAAAGGG 
TCTTTCAGCG AGACGACTGC CGCCGGAAAG AGTTTACCGC AGGCCCAGAA ACTGGAGGAG 
GGCACTGGAC TGGCACCCTT CGATCAGGAG TTCTACTTGA CCTTCGGACT CAGCGTGGGC 
GGTTTTAACG AGTACCAGCA CGAGATCAAA CCCTGGAACG AGAGGGCGCC GCAGGCGCAG 
AAAGCCTTTT GGAAGGAAGT CAAGAAGATC AGGGACCACT GGCTGGACGA GGGCCACATG 
AAGATCGACT ACGTAAAGGT GTACTCTTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523986.2
CDS	6..1478
Translation	MADALRFVAWSCCLQLLFLLLGVQGYEVPKAKIDVFYPKGFEVSIPDEEGITLFAFHGKLNEEMEGLEAGTWARDIVKAKNGRWTFRDRITALKPGDTLYYWTYVIYNGLGYREDDGSFVVNGYSGNNASPHPPVVPVSTTPWTPPADPDIDIRLGCTTPKTEVNGAPTRCAGQLVFVDEFNAAKLDPNKWKAERRFSGQPDYEFNVYVDDAPETLCLANGHVVLSTNTMKKQFKKGSGESLDLGEKCTGQANTHDCVRNGRTLNDGLPPMVTAQFSSKDFSFKYGRVEVRAKMPRAQWVTPQIWLQPRRPIYGVDDYRSGQLRIAYTRPNGGNLDLYGAAVLFADEPLRSVKNCLKPGTGNNSEDWSDSFHNYTLEWTPRELRWLVDGKEWCVQGSAKGSFSETTAAGKSLPQAQKLEEGTGLAPFDQEFYLTFGLSVGGFNEYQHEIKPWNERAPQAQKAFWKEVKKIRDHWLDEGHMKIDYVKVYSL

Target ORF information:

RefSeq Version	NM_079262.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gram-negative bacteria binding protein 3 (GNBP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079262.3

ORF Insert Sequence:

ATGGCGGATG CATTGCGCTT TGTGGCCTGG AGCTGCTGCC TCCAACTGCT GTTCCTGCTC 
CTTGGCGTTC AGGGGTATGA AGTACCCAAG GCTAAGATCG ATGTTTTCTA TCCCAAGGGA 
TTCGAGGTCT CAATTCCCGA TGAGGAGGGC ATCACCCTGT TCGCCTTCCA TGGCAAACTA 
AATGAGGAGA TGGAGGGCCT GGAGGCTGGC ACCTGGGCAC GGGACATTGT CAAAGCGAAA 
AACGGACGCT GGACCTTCCG GGACCGGATT ACGGCTCTAA AGCCTGGCGA CACCCTGTAC 
TACTGGACAT ACGTTATCTA CAACGGACTG GGTTATCGCG AAGACGACGG GTCGTTTGTG 
GTCAATGGGT ACAGTGGTAA TAATGCCTCA CCACATCCAC CTGTGGTTCC AGTCTCCACA 
ACGCCCTGGA CTCCACCTGC CGATCCGGAT ATCGACATAA GGTTGGGTTG TACTACTCCC 
AAAACCGAGG TCAATGGAGC ACCCACTCGC TGTGCCGGGC AGCTGGTGTT CGTGGATGAG 
TTCAATGCTG CAAAGCTGGA TCCCAATAAG TGGAAAGCGG AACGCAGGTT TTCCGGTCAA 
CCCGACTACG AGTTTAATGT GTACGTGGAT GACGCTCCGG AAACCTTGTG CTTAGCCAAT 
GGTCATGTGG TGCTCTCGAC GAACACAATG AAAAAACAAT TCAAGAAGGG TTCGGGTGAA 
AGTTTGGATC TGGGTGAAAA GTGTACGGGA CAAGCGAATA CCCACGACTG TGTAAGAAAT 
GGGCGAACCC TAAATGACGG ACTTCCGCCA ATGGTCACCG CTCAGTTCTC CTCCAAGGAT 
TTCTCCTTCA AGTATGGTCG CGTGGAGGTG CGAGCCAAAA TGCCGCGGGC ACAGTGGGTG 
ACTCCACAAA TCTGGCTGCA GCCCAGGCGT CCGATCTACG GAGTGGATGA TTATCGGTCG 
GGACAGCTGA GAATCGCTTA TACCCGTCCG AATGGAGGTA ATCTGGATTT GTATGGCGCC 
GCCGTGCTCT TTGCAGATGA GCCGCTGCGA TCGGTCAAGA ATTGCCTGAA GCCAGGTACT 
GGAAATAACT CCGAGGACTG GAGCGATAGT TTCCACAATT ACACCCTCGA ATGGACACCC 
AGGGAACTTC GCTGGCTGGT GGACGGCAAG GAGTGGTGTG TCCAGGGAAG TGCTAAAGGG 
TCTTTCAGCG AGACGACTGC CGCCGGAAAG AGTTTACCGC AGGCCCAGAA ACTGGAGGAG 
GGCACTGGAC TGGCACCCTT CGATCAGGAG TTCTACTTGA CCTTCGGACT CAGCGTGGGC 
GGTTTTAACG AGTACCAGCA CGAGATCAAA CCCTGGAACG AGAGGGCGCC GCAGGCGCAG 
AAAGCCTTTT GGAAGGAAGT CAAGAAGATC AGGGACCACT GGCTGGACGA GGGCCACATG 
AAGATCGACT ACGTAAAGGT GTACTCTTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GNBP3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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