Tsp66E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tsp66E
Entrez Gene ID	39017
Full Name	Tetraspanin 66E
Synonyms	CG4999,Dm.Tsp66E,Dmel\CG4999
General protein information	Preferred Names Tetraspanin 66E Names CG4999 gene product from transcript CG4999-RB CG4999-PA CG4999-PB CG4999-PC Tsp66E-PA Tsp66E-PB Tsp66E-PC tetraspanin 66E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E3-66E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168298.1	NP_729423.1	tetraspanin 66E, isoform B
NM_079261.5	NP_523985.1	tetraspanin 66E, isoform A
NM_168299.2	NP_729424.1	tetraspanin 66E, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD82	NP_001008470.1
Danio rerio (zebrafish)	cd82b	NP_997828.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004861	XP_314330.2
Pan troglodytes (chimpanzee)	CD82	XP_001159791.1
Danio rerio (zebrafish)	cd82a	NP_997826.1
Drosophila melanogaster (fruit fly)	Tsp66E	NP_523985.1
Homo sapiens (human)	CD82	NP_002222.1
	CD82	XP_002799617.1
Bos taurus (cattle)	LOC100335281	XP_005216571.1
Xenopus tropicalis (tropical clawed frog)	cd82	NP_989191.1
Canis lupus familiaris (dog)	CD82	XP_005631367.1
Rattus norvegicus (Norway rat)	Cd82	NP_113985.2
Mus musculus (house mouse)	Cd82	NP_001258360.1
Caenorhabditis elegans (roundworm)	tsp-8	NP_510445.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Tsp66E and Tsp74F mutant ovaries had elevated inflated gene product alphaPS2 integrin levels and reduced endocytic trafficking.
Title: Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization.

The following Tsp66E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsp66E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17957	NM_168298.1 Latest version!	Drosophila melanogaster tetraspanin 66E, transcript variant B (Tsp66E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa17957	NM_079261.5 Latest version!	Drosophila melanogaster tetraspanin 66E, transcript variant A (Tsp66E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa17957	NM_168299.2 Latest version!	Drosophila melanogaster tetraspanin 66E, transcript variant C (Tsp66E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tsp66E ( NM_168298.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729423.1 Drosophila melanogaster tetraspanin 66E, transcript variant B (Tsp66E), mRNA.

Price & Availability↑

CloneID	OFa17957
Clone ID Related Accession (Same CDS sequence)	NM_168298.1 , NM_079261.5 , NM_168299.2
Accession Version	NM_168298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetraspanin 66E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729423.1
CDS	236..1039
Translation	MVFDCGVWCAKYLLCIFNFIFFVLGTIIFGVGLWLAVDKHSLIALLKLVESERIEQFTQPQAIEQLAYVLLVIGAVMFFMSFLGYLGAMRESRCLLSTYGTFLILLLIAEIVAGGLGAFFKDKVRAESKNFLQTTITSYSLGENVDATSLMWNQLMGNFGCCGINDYHDFDASPAWVNGKGNRTIPDACCILKDVAKLVPRDEDCTTNPSDSNSFYKKGCYEVFTEWLIRQRELVIVAIAVGIVHLVLIILAFALCKAFAKYNDMRL

Target ORF information:

RefSeq Version	NM_168298.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetraspanin 66E, transcript variant B (Tsp66E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168298.1

ORF Insert Sequence:

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp66E ( NM_079261.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523985.1 Drosophila melanogaster tetraspanin 66E, transcript variant A (Tsp66E), mRNA.

Price & Availability↑

CloneID	OFa17957
Clone ID Related Accession (Same CDS sequence)	NM_168298.1 , NM_079261.5 , NM_168299.2
Accession Version	NM_079261.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetraspanin 66E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079261.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523985.1
CDS	342..1145
Translation	MVFDCGVWCAKYLLCIFNFIFFVLGTIIFGVGLWLAVDKHSLIALLKLVESERIEQFTQPQAIEQLAYVLLVIGAVMFFMSFLGYLGAMRESRCLLSTYGTFLILLLIAEIVAGGLGAFFKDKVRAESKNFLQTTITSYSLGENVDATSLMWNQLMGNFGCCGINDYHDFDASPAWVNGKGNRTIPDACCILKDVAKLVPRDEDCTTNPSDSNSFYKKGCYEVFTEWLIRQRELVIVAIAVGIVHLVLIILAFALCKAFAKYNDMRL

Target ORF information:

RefSeq Version	NM_079261.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetraspanin 66E, transcript variant A (Tsp66E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079261.5

ORF Insert Sequence:

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp66E ( NM_168299.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729424.1 Drosophila melanogaster tetraspanin 66E, transcript variant C (Tsp66E), mRNA.

Price & Availability↑

CloneID	OFa17957
Clone ID Related Accession (Same CDS sequence)	NM_168298.1 , NM_079261.5 , NM_168299.2
Accession Version	NM_168299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetraspanin 66E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168299.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729424.1
CDS	368..1171
Translation	MVFDCGVWCAKYLLCIFNFIFFVLGTIIFGVGLWLAVDKHSLIALLKLVESERIEQFTQPQAIEQLAYVLLVIGAVMFFMSFLGYLGAMRESRCLLSTYGTFLILLLIAEIVAGGLGAFFKDKVRAESKNFLQTTITSYSLGENVDATSLMWNQLMGNFGCCGINDYHDFDASPAWVNGKGNRTIPDACCILKDVAKLVPRDEDCTTNPSDSNSFYKKGCYEVFTEWLIRQRELVIVAIAVGIVHLVLIILAFALCKAFAKYNDMRL

Target ORF information:

RefSeq Version	NM_168299.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetraspanin 66E, transcript variant C (Tsp66E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168299.2

ORF Insert Sequence:

ATGGTCTTCG ACTGCGGAGT CTGGTGTGCA AAGTATTTGC TTTGCATATT CAATTTTATA 
TTTTTCGTGC TAGGCACAAT TATATTCGGA GTCGGACTAT GGCTGGCCGT GGACAAGCAC 
TCGCTGATTG CTCTCCTCAA GTTGGTCGAG AGCGAGCGCA TTGAGCAATT TACACAGCCA 
CAGGCCATAG AGCAGCTGGC CTATGTGCTC CTCGTCATCG GAGCCGTCAT GTTCTTCATG 
AGCTTCTTGG GCTATCTGGG AGCCATGCGC GAGTCCCGCT GTCTGCTGTC GACGTATGGA 
ACCTTTTTGA TCCTGCTGCT GATTGCCGAG ATCGTTGCCG GCGGATTGGG TGCCTTCTTC 
AAGGACAAAG TTCGTGCCGA GAGCAAAAAC TTCTTGCAGA CGACCATTAC CAGTTATTCG 
CTGGGCGAGA ATGTGGATGC TACCTCACTG ATGTGGAACC AACTGATGGG CAACTTTGGG 
TGCTGTGGCA TCAATGACTA TCACGATTTT GATGCCTCTC CAGCGTGGGT GAACGGCAAG 
GGTAACCGCA CCATTCCCGA TGCCTGCTGT ATCCTTAAGG ATGTGGCCAA GTTGGTGCCA 
CGCGACGAGG ACTGTACAAC CAACCCCAGC GACAGCAACA GTTTCTACAA GAAGGGCTGC 
TATGAGGTGT TTACCGAGTG GCTGATTCGG CAACGTGAAC TGGTCATCGT GGCCATTGCG 
GTGGGCATTG TGCACCTGGT CCTCATTATT CTGGCCTTTG CTCTGTGCAA GGCCTTTGCC 
AAATACAACG ATATGCGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tsp66E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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