Sh3beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh3beta
Entrez Gene ID	38820
Full Name	CG8582 gene product from transcript CG8582-RD
Synonyms	CG8582,Dmel\CG8582,LD12912,SH3beta
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65F7-65F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_168210.3	NP_729248.1	Sh3beta, isoform A
NM_001202127.2	NP_001189056.1	Sh3beta, isoform D
NM_001202128.2	NP_001189057.1	Sh3beta, isoform E
NM_139855.4	NP_648112.2	Sh3beta, isoform B
NM_001202126.1	NP_001189055.1	Sh3beta, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sh3beta gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3beta cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17791	NM_001202127.2 Latest version!	Drosophila melanogaster Sh3beta, transcript variant D (Sh3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa17793	NM_001202126.1 Latest version!	Drosophila melanogaster Sh3beta, transcript variant C (Sh3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa20642	NM_139855.4 Latest version!	Drosophila melanogaster Sh3beta, transcript variant B (Sh3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa20642	NM_168210.3 Latest version!	Drosophila melanogaster Sh3beta, transcript variant A (Sh3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17792	NM_001202128.2 Latest version!	Drosophila melanogaster Sh3beta, transcript variant E (Sh3beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh3beta ( NM_001202127.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189056.1 Drosophila melanogaster Sh3beta, transcript variant D (Sh3beta), mRNA.

Price & Availability↑

CloneID	OFa17791
Clone ID Related Accession (Same CDS sequence)	NM_001202127.2
Accession Version	NM_001202127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sh3beta, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202127.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GAACGAAGAC 
GGCAAGGACA AGGAGAATTC AAAGGGCGAG GATGAAGACG CAGATGAAGA GAATGCCGAG 
CAAAAGAAGT TACCAGCGAC CACAACAACA ACAGCAGCAT CAGCTAAGAA GACTTCAGAT 
TCCCAATCGG ATCTCTTATT GGACAGCGAG CGGGCAGCGG CTGCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189056.1
CDS	327..977
Translation	MVLKVYVSGMSGNKEVKKRQQRVLMILDSKNIKYDTVDITEPGKESEKELMQNKSTSNGGTVSDPEPRHPLPPQLFNDDEYCGDYDAFDMANEIDTLEVFLKLAPADTTAVSTAQIELKQENGDAKKEEAETEAEDKKTEAGDGDVDVKEEAAEKAENEDGKDKENSKGEDEDADEENAEQKKLPATTTTTAASAKKTSDSQSDLLLDSERAAAAQ

Target ORF information:

RefSeq Version	NM_001202127.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sh3beta, transcript variant D (Sh3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202127.2

ORF Insert Sequence:

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GAACGAAGAC 
GGCAAGGACA AGGAGAATTC AAAGGGCGAG GATGAAGACG CAGATGAAGA GAATGCCGAG 
CAAAAGAAGT TACCAGCGAC CACAACAACA ACAGCAGCAT CAGCTAAGAA GACTTCAGAT 
TCCCAATCGG ATCTCTTATT GGACAGCGAG CGGGCAGCGG CTGCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3beta ( NM_001202126.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189055.1 Drosophila melanogaster Sh3beta, transcript variant C (Sh3beta), mRNA.

Price & Availability↑

CloneID	OFa17793
Clone ID Related Accession (Same CDS sequence)	NM_001202126.1
Accession Version	NM_001202126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sh3beta, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAAGG TGAAGAAGCG CCAGCAGCGC GTTCTGATGA TCCTGGACAG CAAGAACATC 
AAGTACGACA CGGTGGACAT CACGGAGCCC GGCAAGGAAT CCGAGAAGGA GCTGATGCAA 
AACAAATCGA CAAGCAACGG TGGAACGGTC AGCGATCCAG AGCCCCGTCA TCCGCTTCCT 
CCGCAGCTGT TCAACGACGA CGAGTACTGC GGTGATTACG ACGCCTTTGA CATGGCCAAC 
GAGATCGATA CCCTGGAGGT GTTCCTCAAG CTGGCTCCAG CCGACACCAC GGCAGTTAGT 
ACCGCCCAAA TCGAACTGAA GCAGGAGAAC GGCGATGCCA AGAAGGAGGA GGCGGAAACG 
GAGGCGGAGG ATAAGAAAAC GGAGGCCGGC GATGGCGATG TGGATGTCAA GGAGGAGGCT 
GCTGAGAAGG CCGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189055.1
CDS	120..560
Translation	MLKVKKRQQRVLMILDSKNIKYDTVDITEPGKESEKELMQNKSTSNGGTVSDPEPRHPLPPQLFNDDEYCGDYDAFDMANEIDTLEVFLKLAPADTTAVSTAQIELKQENGDAKKEEAETEAEDKKTEAGDGDVDVKEEAAEKAEV

Target ORF information:

RefSeq Version	NM_001202126.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sh3beta, transcript variant C (Sh3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202126.1

ORF Insert Sequence:

ATGTTAAAGG TGAAGAAGCG CCAGCAGCGC GTTCTGATGA TCCTGGACAG CAAGAACATC 
AAGTACGACA CGGTGGACAT CACGGAGCCC GGCAAGGAAT CCGAGAAGGA GCTGATGCAA 
AACAAATCGA CAAGCAACGG TGGAACGGTC AGCGATCCAG AGCCCCGTCA TCCGCTTCCT 
CCGCAGCTGT TCAACGACGA CGAGTACTGC GGTGATTACG ACGCCTTTGA CATGGCCAAC 
GAGATCGATA CCCTGGAGGT GTTCCTCAAG CTGGCTCCAG CCGACACCAC GGCAGTTAGT 
ACCGCCCAAA TCGAACTGAA GCAGGAGAAC GGCGATGCCA AGAAGGAGGA GGCGGAAACG 
GAGGCGGAGG ATAAGAAAAC GGAGGCCGGC GATGGCGATG TGGATGTCAA GGAGGAGGCT 
GCTGAGAAGG CCGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3beta ( NM_168210.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729248.1 Drosophila melanogaster Sh3beta, transcript variant A (Sh3beta), mRNA.

Price & Availability↑

CloneID	OFa20642
Clone ID Related Accession (Same CDS sequence)	NM_168210.3 , NM_139855.4
Accession Version	NM_168210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sh3beta, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168210.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729248.1
CDS	231..707
Translation	MVLKVYVSGMSGNKEVKKRQQRVLMILDSKNIKYDTVDITEPGKESEKELMQNKSTSNGGTVSDPEPRHPLPPQLFNDDEYCGDYDAFDMANEIDTLEVFLKLAPADTTAVSTAQIELKQENGDAKKEEAETEAEDKKTEAGDGDVDVKEEAAEKAEV

Target ORF information:

RefSeq Version	NM_168210.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sh3beta, transcript variant A (Sh3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168210.3

ORF Insert Sequence:

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3beta ( NM_139855.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648112.2 Drosophila melanogaster Sh3beta, transcript variant B (Sh3beta), mRNA.

Price & Availability↑

CloneID	OFa20642
Clone ID Related Accession (Same CDS sequence)	NM_168210.3 , NM_139855.4
Accession Version	NM_139855.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sh3beta, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139855.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648112.2
CDS	327..803
Translation	MVLKVYVSGMSGNKEVKKRQQRVLMILDSKNIKYDTVDITEPGKESEKELMQNKSTSNGGTVSDPEPRHPLPPQLFNDDEYCGDYDAFDMANEIDTLEVFLKLAPADTTAVSTAQIELKQENGDAKKEEAETEAEDKKTEAGDGDVDVKEEAAEKAEV

Target ORF information:

RefSeq Version	NM_139855.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sh3beta, transcript variant B (Sh3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139855.4

ORF Insert Sequence:

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3beta ( NM_001202128.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189057.1 Drosophila melanogaster Sh3beta, transcript variant E (Sh3beta), mRNA.

Price & Availability↑

CloneID	OFa17792
Clone ID Related Accession (Same CDS sequence)	NM_001202128.2
Accession Version	NM_001202128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sh3beta, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202128.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GAACGAAGAC 
GGCAAGGACA AGGAGAATTC AAAGGGCGAG GATGAAGACG CAGATGAAGA GAATGCCGAG 
CAAAAGACTG AAACCGAAGA ATCTGGGGAC AAGAAAACGG AGGAGAAAAC TACAGACGCT 
GAAGGTGAGA CCCAAGAAAA GTCTGAGGAA GTGGATACCA AGGAAAAATC AGAGGTTGAG 
AGTACCAAAG ATGATGAGGT TGGAAAATCC GAGGATGTTA AAGATGATGA GGATGAAAAA 
TCCGAGATTG TAAAAGATGA TAAGGAAAAC TCCCAGGATG TTAAGGATGA TAAAACTGAA 
AAATCCGAGG ATGTTAAAGA TAAATTAGAG GGAAGTGAGG AGACCGCAGA AACATCGGAG 
GATGCTGACA CTACTGAGAA ACCAGATGTT GAGGCCACCA ATGATGATGG GGATACAAAA 
TCGGAGAAAA ACGAGACGCA AGAAAAGTCC GAGGAAGTTG ATGCCGTTGA AGAATCAGAG 
GCCGAAAGCT CCAAGGAAAA AACGGAGGAA AATGACACCA AAGGCACCAA AGTCAATGAG 
AATGATAAAG ACGAGGATGT TGAAGAAAAG ACCGAGGAAA ATGCAACAGA AGAGTCCGCG 
GACTCGGATA GTGCTACCAA ATCAGAGGTT AAAAGTACCG AAGATGATGA GGTTGTAGAA 
TCGGAGGATG TGAAAGATGA ATTCGAAGAA AATGAGACCA ACAAAAAGGT CGAGGAAGTT 
GGTAGTACTG AAATATCCGA GGTTGTAAGT ACGGAAAACG AAAAATTTGA AGATATTAAA 
GAAAATCCTA ATGAAAATGA GGCTCAAGAA AAGCCCGAAG AAGTTATGAC TGCCGAAAGA 
TCTGTAGAGG AAACCAAAGA GGAAGAACCA ATCGACGAGG AAAAACCTGC AGAAACGAAG 
ACAGAAGACA CGCCCGAAAA TGAAGAAAGC AAACCGGATG GCGAGAATTC CTCTTCCGAA 
GAAAGCGAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189057.1
CDS	327..1784
Translation	MVLKVYVSGMSGNKEVKKRQQRVLMILDSKNIKYDTVDITEPGKESEKELMQNKSTSNGGTVSDPEPRHPLPPQLFNDDEYCGDYDAFDMANEIDTLEVFLKLAPADTTAVSTAQIELKQENGDAKKEEAETEAEDKKTEAGDGDVDVKEEAAEKAENEDGKDKENSKGEDEDADEENAEQKTETEESGDKKTEEKTTDAEGETQEKSEEVDTKEKSEVESTKDDEVGKSEDVKDDEDEKSEIVKDDKENSQDVKDDKTEKSEDVKDKLEGSEETAETSEDADTTEKPDVEATNDDGDTKSEKNETQEKSEEVDAVEESEAESSKEKTEENDTKGTKVNENDKDEDVEEKTEENATEESADSDSATKSEVKSTEDDEVVESEDVKDEFEENETNKKVEEVGSTEISEVVSTENEKFEDIKENPNENEAQEKPEEVMTAERSVEETKEEEPIDEEKPAETKTEDTPENEESKPDGENSSSEESEEE

Target ORF information:

RefSeq Version	NM_001202128.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sh3beta, transcript variant E (Sh3beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202128.2

ORF Insert Sequence:

ATGGTCTTGA AAGTCTACGT CAGCGGCATG TCCGGCAATA AGGAGGTGAA GAAGCGCCAG 
CAGCGCGTTC TGATGATCCT GGACAGCAAG AACATCAAGT ACGACACGGT GGACATCACG 
GAGCCCGGCA AGGAATCCGA GAAGGAGCTG ATGCAAAACA AATCGACAAG CAACGGTGGA 
ACGGTCAGCG ATCCAGAGCC CCGTCATCCG CTTCCTCCGC AGCTGTTCAA CGACGACGAG 
TACTGCGGTG ATTACGACGC CTTTGACATG GCCAACGAGA TCGATACCCT GGAGGTGTTC 
CTCAAGCTGG CTCCAGCCGA CACCACGGCA GTTAGTACCG CCCAAATCGA ACTGAAGCAG 
GAGAACGGCG ATGCCAAGAA GGAGGAGGCG GAAACGGAGG CGGAGGATAA GAAAACGGAG 
GCCGGCGATG GCGATGTGGA TGTCAAGGAG GAGGCTGCTG AGAAGGCCGA GAACGAAGAC 
GGCAAGGACA AGGAGAATTC AAAGGGCGAG GATGAAGACG CAGATGAAGA GAATGCCGAG 
CAAAAGACTG AAACCGAAGA ATCTGGGGAC AAGAAAACGG AGGAGAAAAC TACAGACGCT 
GAAGGTGAGA CCCAAGAAAA GTCTGAGGAA GTGGATACCA AGGAAAAATC AGAGGTTGAG 
AGTACCAAAG ATGATGAGGT TGGAAAATCC GAGGATGTTA AAGATGATGA GGATGAAAAA 
TCCGAGATTG TAAAAGATGA TAAGGAAAAC TCCCAGGATG TTAAGGATGA TAAAACTGAA 
AAATCCGAGG ATGTTAAAGA TAAATTAGAG GGAAGTGAGG AGACCGCAGA AACATCGGAG 
GATGCTGACA CTACTGAGAA ACCAGATGTT GAGGCCACCA ATGATGATGG GGATACAAAA 
TCGGAGAAAA ACGAGACGCA AGAAAAGTCC GAGGAAGTTG ATGCCGTTGA AGAATCAGAG 
GCCGAAAGCT CCAAGGAAAA AACGGAGGAA AATGACACCA AAGGCACCAA AGTCAATGAG 
AATGATAAAG ACGAGGATGT TGAAGAAAAG ACCGAGGAAA ATGCAACAGA AGAGTCCGCG 
GACTCGGATA GTGCTACCAA ATCAGAGGTT AAAAGTACCG AAGATGATGA GGTTGTAGAA 
TCGGAGGATG TGAAAGATGA ATTCGAAGAA AATGAGACCA ACAAAAAGGT CGAGGAAGTT 
GGTAGTACTG AAATATCCGA GGTTGTAAGT ACGGAAAACG AAAAATTTGA AGATATTAAA 
GAAAATCCTA ATGAAAATGA GGCTCAAGAA AAGCCCGAAG AAGTTATGAC TGCCGAAAGA 
TCTGTAGAGG AAACCAAAGA GGAAGAACCA ATCGACGAGG AAAAACCTGC AGAAACGAAG 
ACAGAAGACA CGCCCGAAAA TGAAGAAAGC AAACCGGATG GCGAGAATTC CTCTTCCGAA 
GAAAGCGAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sh3beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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