eco cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eco
Entrez Gene ID	38812
Full Name	Establishment of cohesion
Synonyms	CG8598,Deco,Dmel\CG8598,ECO,Eco1,deco
General protein information	Preferred Names Establishment of cohesion Names CG8598 gene product from transcript CG8598-RA CG8598-PA CG8598-PB drosophila eco1 eco-PA eco-PB establishment of cohesion
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65F5-65F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_139849.3	NP_648106.1	establishment of cohesion, isoform A
NM_168201.2	NP_729236.2	establishment of cohesion, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eco gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eco cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20666	NM_139849.3 Latest version!	Drosophila melanogaster establishment of cohesion, transcript variant A (eco), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20666	NM_168201.2 Latest version!	Drosophila melanogaster establishment of cohesion, transcript variant B (eco), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eco ( NM_139849.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648106.1 Drosophila melanogaster establishment of cohesion, transcript variant A (eco), mRNA.

Price & Availability↑

CloneID	OFa20666
Clone ID Related Accession (Same CDS sequence)	NM_139849.3 , NM_168201.2
Accession Version	NM_139849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	establishment of cohesion, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139849.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGACCC CGACGGGTAG TGGGCGTCCC AGTCGCATGG CCACTCCGCG CCTTTCGGAG 
CGCAAGCGGC AACTGTTTGG CAGTCCCAGA TCCAGGCTGC GCCAAATCAA CGACGACGAG 
GATGATGCGG ACGTAGACAG TCTGGGTGTC TTGCCATTAA AGACCCATGT GGCGGCCAAC 
AGGAAGGGCA GGAGTCTCTT CGCCGCAGTC CCCGGCAAGT CAAGCAGCAG TGCGAACAGC 
AGCCCGGAAA CCAACAAGGA GAACAAAAAA ACCCGTGGCG GAGTCATGAC TGCCACGGCG 
GAGCAGCTGC CCCAATTGTT TACAGCCACC ATGCGGCTAA ATAGCAACAG CAGCAGCAAT 
AGTCGCAACA GCAGCCCCCG TCAGACGAGG GTCCAAAGAA AGCGAGCGGA TTCTTCAATG 
TCGTCGCCCA CCTCTTCCTC TGAAGGAACT CCTTCTTCCA GAGCCAGAAA TAGCATCAGA 
CGCAGTCCAC GAACCTTCAG TGCGCAAAAG GATCCAGATG CGTTCTCCTC GCCAGAATCT 
TTTCAGACTA GGCTAAGCAA GGTGGCAGCT ATGCTAATGA AGGGTCAGGA TTCAAGATCA 
ATGTTAGAAA AATCAAAGAA GAAACACAAC CACAGCCTAA AGACCACTGC CCAAGTGCAT 
ACAACTAAAC CGAAGAAAAC GAGCCCAGCT GAAGAGTCCC AGTCGGACGA CGAGAAACCT 
TCTAGCAGTA AGAACTCCAG GAAGAATACA GAAGTTCGTG AAACAAGGAG CTCGCAAATC 
ATCAGTCCAA AAACCAGGAA TAGGCGAAGA CCCTTCACGT CGGCGGATAT AAACTGCAAG 
ACGCTGAAGG CTGCTGCCCA TCTACATGAG AACATGAGGT CGTATGATGA AGAAAAAACA 
GCAGCAGTTA AGCTAGAAAA CAGCAGAAGC AGATCCAAGT CCCCGGTAGA AGTGTTCAAG 
TCAAATGACG ATGCAGTAAA ACGAAACACA GGAAACACCA ACAATAAAAC AGCGAAATCT 
TCCGAAGTTG CCACAGCAAA AAGGCCAGAA AGCCCAGGAA GTAGTATGAA GATTGATGTC 
GAAGTGCCCG AATCGGACGA AGAAGCATCC AATCATAAGC CTCAAAAGCG ACAGCACCCA 
GAAACGTCCA CCCCGGTTGC TCCTTCAGCG GACGCCGACT CTGGTTCGCC GCAGAGCAAG 
ATGCGCAAGG TGACGCTGAG CAGCAGCATT CCCACCATGG CCTTCTATTC CCACAGTGGA 
GAAGCGGTCA CAAAGTCAAG AAGGCGGCCG TCCATATCCA AGAATTCGCT TAAACAACCA 
ACGAAAATCT CGCCAACCTC CCGTCCACTA TTGGGCATTA ATAAGGGAGT TCACCACAAA 
ATACGCAAGC GTCACGGTTT TGCCAACCGA CTGCCAGCCA CCGATATGGA CAACATTCTC 
AACTCGCTGA GCAACGAACG GCTCAAGAAC CTGATCACCA CCAAGCGTGA AGAGCGCGCC 
AAAGTGGAGG AAGTCCACCA GATTCTCCGT AATGCCAAGG ACCCGATCAA AATGGCCAAA 
CCATTGAGTG TGATTGAAGC CGACGATGCT AACAACAATA ACAATCTGCC AGCAACCGCA 
TGGCAAGAAA CTTCCGCCGA TTTCTCCGAT CTTAGTGATG TCGAAGACAT TGATCCCATC 
ATCGAGGTGG AGCCTATCAT ACCAATAATT CGCCACGAGC CGGTCCAGAA ATCCCCAACC 
GCCGAGCCAG CCGATCTAAG CAAACGAAAG TTCTTCAAAT CGGGCCGCCG TAGTAGCACC 
TGCATGGAGG TGAGAATCAC GGACAACATC CGGGCCAGCG TCAGCCAGGG CAAGATTGAA 
CTCGTCCAAA CGATACGCCG TAAGCCGCGG CAAGTGCGCG TCAAGTCGGC CACGATATTT 
TCCGCCGAGC AGGCGACTGT GGATGCGATC CTAAAGAATC TGGACGATAC GGTGGTTGAT 
GAGATTGTAG AAGCGAATCC GGTGGTACAG GCAACGCCCA TTGATGCGGA GGAGACGACC 
ATGGAAACGG AGTCGCTACC GGATATAATT GAGTATGCCC CAGAGGCGAA CGATGTGGAA 
ATTGATCCCT TTGCTGAGTT CCGTCAACGT TTGCCATACC AAACCGATGA TCCCAATGTC 
GTGGAGCAAC AGCAAATTTT ACTAGAGTTT CTCATTAGCA ACAATATTTG CACCGAAAAG 
AACTTTGAGA TATTTATTGC AAATCCGGAT GACTACAAGG ACGAGGCCAA TCAAATTGTG 
GACAATTTAT ACATGGTGGT GAACAGCGAG GAGGCTGCGC AGCTTGCTCA GATGGAAACA 
GTGGAAAATA CAGCGGTGGC CATTGCACCA AAACAGGATG CGCCAGCAGT AGAGGAAGTT 
CAGCCAAAGC TTTTCCCCAT ATTCACTCAA CGCTTACAGC CCGTTGTCCA GAAATCGTTG 
CGGCGTCGGC CGGATACATC GATGAGATTG CTAACGGCGG CGGGTGGATC GAATCAGTAC 
CAGATCGATG CTGGTCAAAA GGCCTTCGGA GCACGGCAGT GCCAGCAGTG CGGATTGGTG 
TACACCGTGC ACGAGCCGGA GGAGGAGCTG CTTCATCGCG AGTACCACAA TTCCATCCAT 
GTGCTGCGCT TCAAGGGCTG GATCGACGAG GACATTGTGT CCGTTTATCC AGAATGGGCC 
AGCGACGGGC GCATCATACG GATCAACGAA CGCGCTCCGA CAGCTCGGCT CGACAGACTG 
CGGGATCTCA TTGGTGTGGT GGACAAAGAG CTGGGCTATT CCTCGTACAT TGTGCCAAAG 
ATCTTTGTAG CCTTCATAGC AGTGCGCAAG CAGCAGATCG TAGGCTTCTG CCTGGTGCAG 
CCCCTCTCGC AGGCCCATCG TTTCATCCAG GTCGATGGCA CGGACTACTT CAGCGAGGAA 
AGCTACCCGG CCAGCTGCGG CGTCTCACGC ATCTGGGTTT CTCCGCTGCA GCGACGCAGC 
GGGATCGCCA GCAAGCTGCT GCGAGTCGTC CAGTGCCACA CGGTGCTCGG TCAAGAGATC 
GCTAGGGAGT GCATTGCCTT CAGTACGCCC ACCGACGATG GTCGGGCTCT GGCACGCCAG 
TTCACCGGGT TGGATAACTT CCTCACCTAC GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648106.1
CDS	267..3425
Translation	METPTGSGRPSRMATPRLSERKRQLFGSPRSRLRQINDDEDDADVDSLGVLPLKTHVAANRKGRSLFAAVPGKSSSSANSSPETNKENKKTRGGVMTATAEQLPQLFTATMRLNSNSSSNSRNSSPRQTRVQRKRADSSMSSPTSSSEGTPSSRARNSIRRSPRTFSAQKDPDAFSSPESFQTRLSKVAAMLMKGQDSRSMLEKSKKKHNHSLKTTAQVHTTKPKKTSPAEESQSDDEKPSSSKNSRKNTEVRETRSSQIISPKTRNRRRPFTSADINCKTLKAAAHLHENMRSYDEEKTAAVKLENSRSRSKSPVEVFKSNDDAVKRNTGNTNNKTAKSSEVATAKRPESPGSSMKIDVEVPESDEEASNHKPQKRQHPETSTPVAPSADADSGSPQSKMRKVTLSSSIPTMAFYSHSGEAVTKSRRRPSISKNSLKQPTKISPTSRPLLGINKGVHHKIRKRHGFANRLPATDMDNILNSLSNERLKNLITTKREERAKVEEVHQILRNAKDPIKMAKPLSVIEADDANNNNNLPATAWQETSADFSDLSDVEDIDPIIEVEPIIPIIRHEPVQKSPTAEPADLSKRKFFKSGRRSSTCMEVRITDNIRASVSQGKIELVQTIRRKPRQVRVKSATIFSAEQATVDAILKNLDDTVVDEIVEANPVVQATPIDAEETTMETESLPDIIEYAPEANDVEIDPFAEFRQRLPYQTDDPNVVEQQQILLEFLISNNICTEKNFEIFIANPDDYKDEANQIVDNLYMVVNSEEAAQLAQMETVENTAVAIAPKQDAPAVEEVQPKLFPIFTQRLQPVVQKSLRRRPDTSMRLLTAAGGSNQYQIDAGQKAFGARQCQQCGLVYTVHEPEEELLHREYHNSIHVLRFKGWIDEDIVSVYPEWASDGRIIRINERAPTARLDRLRDLIGVVDKELGYSSYIVPKIFVAFIAVRKQQIVGFCLVQPLSQAHRFIQVDGTDYFSEESYPASCGVSRIWVSPLQRRSGIASKLLRVVQCHTVLGQEIARECIAFSTPTDDGRALARQFTGLDNFLTYDQ

Target ORF information:

RefSeq Version	NM_139849.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster establishment of cohesion, transcript variant A (eco), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139849.3

ORF Insert Sequence:

ATGGAGACCC CGACGGGTAG TGGGCGTCCC AGTCGCATGG CCACTCCGCG CCTTTCGGAG 
CGCAAGCGGC AACTGTTTGG CAGTCCCAGA TCCAGGCTGC GCCAAATCAA CGACGACGAG 
GATGATGCGG ACGTAGACAG TCTGGGTGTC TTGCCATTAA AGACCCATGT GGCGGCCAAC 
AGGAAGGGCA GGAGTCTCTT CGCCGCAGTC CCCGGCAAGT CAAGCAGCAG TGCGAACAGC 
AGCCCGGAAA CCAACAAGGA GAACAAAAAA ACCCGTGGCG GAGTCATGAC TGCCACGGCG 
GAGCAGCTGC CCCAATTGTT TACAGCCACC ATGCGGCTAA ATAGCAACAG CAGCAGCAAT 
AGTCGCAACA GCAGCCCCCG TCAGACGAGG GTCCAAAGAA AGCGAGCGGA TTCTTCAATG 
TCGTCGCCCA CCTCTTCCTC TGAAGGAACT CCTTCTTCCA GAGCCAGAAA TAGCATCAGA 
CGCAGTCCAC GAACCTTCAG TGCGCAAAAG GATCCAGATG CGTTCTCCTC GCCAGAATCT 
TTTCAGACTA GGCTAAGCAA GGTGGCAGCT ATGCTAATGA AGGGTCAGGA TTCAAGATCA 
ATGTTAGAAA AATCAAAGAA GAAACACAAC CACAGCCTAA AGACCACTGC CCAAGTGCAT 
ACAACTAAAC CGAAGAAAAC GAGCCCAGCT GAAGAGTCCC AGTCGGACGA CGAGAAACCT 
TCTAGCAGTA AGAACTCCAG GAAGAATACA GAAGTTCGTG AAACAAGGAG CTCGCAAATC 
ATCAGTCCAA AAACCAGGAA TAGGCGAAGA CCCTTCACGT CGGCGGATAT AAACTGCAAG 
ACGCTGAAGG CTGCTGCCCA TCTACATGAG AACATGAGGT CGTATGATGA AGAAAAAACA 
GCAGCAGTTA AGCTAGAAAA CAGCAGAAGC AGATCCAAGT CCCCGGTAGA AGTGTTCAAG 
TCAAATGACG ATGCAGTAAA ACGAAACACA GGAAACACCA ACAATAAAAC AGCGAAATCT 
TCCGAAGTTG CCACAGCAAA AAGGCCAGAA AGCCCAGGAA GTAGTATGAA GATTGATGTC 
GAAGTGCCCG AATCGGACGA AGAAGCATCC AATCATAAGC CTCAAAAGCG ACAGCACCCA 
GAAACGTCCA CCCCGGTTGC TCCTTCAGCG GACGCCGACT CTGGTTCGCC GCAGAGCAAG 
ATGCGCAAGG TGACGCTGAG CAGCAGCATT CCCACCATGG CCTTCTATTC CCACAGTGGA 
GAAGCGGTCA CAAAGTCAAG AAGGCGGCCG TCCATATCCA AGAATTCGCT TAAACAACCA 
ACGAAAATCT CGCCAACCTC CCGTCCACTA TTGGGCATTA ATAAGGGAGT TCACCACAAA 
ATACGCAAGC GTCACGGTTT TGCCAACCGA CTGCCAGCCA CCGATATGGA CAACATTCTC 
AACTCGCTGA GCAACGAACG GCTCAAGAAC CTGATCACCA CCAAGCGTGA AGAGCGCGCC 
AAAGTGGAGG AAGTCCACCA GATTCTCCGT AATGCCAAGG ACCCGATCAA AATGGCCAAA 
CCATTGAGTG TGATTGAAGC CGACGATGCT AACAACAATA ACAATCTGCC AGCAACCGCA 
TGGCAAGAAA CTTCCGCCGA TTTCTCCGAT CTTAGTGATG TCGAAGACAT TGATCCCATC 
ATCGAGGTGG AGCCTATCAT ACCAATAATT CGCCACGAGC CGGTCCAGAA ATCCCCAACC 
GCCGAGCCAG CCGATCTAAG CAAACGAAAG TTCTTCAAAT CGGGCCGCCG TAGTAGCACC 
TGCATGGAGG TGAGAATCAC GGACAACATC CGGGCCAGCG TCAGCCAGGG CAAGATTGAA 
CTCGTCCAAA CGATACGCCG TAAGCCGCGG CAAGTGCGCG TCAAGTCGGC CACGATATTT 
TCCGCCGAGC AGGCGACTGT GGATGCGATC CTAAAGAATC TGGACGATAC GGTGGTTGAT 
GAGATTGTAG AAGCGAATCC GGTGGTACAG GCAACGCCCA TTGATGCGGA GGAGACGACC 
ATGGAAACGG AGTCGCTACC GGATATAATT GAGTATGCCC CAGAGGCGAA CGATGTGGAA 
ATTGATCCCT TTGCTGAGTT CCGTCAACGT TTGCCATACC AAACCGATGA TCCCAATGTC 
GTGGAGCAAC AGCAAATTTT ACTAGAGTTT CTCATTAGCA ACAATATTTG CACCGAAAAG 
AACTTTGAGA TATTTATTGC AAATCCGGAT GACTACAAGG ACGAGGCCAA TCAAATTGTG 
GACAATTTAT ACATGGTGGT GAACAGCGAG GAGGCTGCGC AGCTTGCTCA GATGGAAACA 
GTGGAAAATA CAGCGGTGGC CATTGCACCA AAACAGGATG CGCCAGCAGT AGAGGAAGTT 
CAGCCAAAGC TTTTCCCCAT ATTCACTCAA CGCTTACAGC CCGTTGTCCA GAAATCGTTG 
CGGCGTCGGC CGGATACATC GATGAGATTG CTAACGGCGG CGGGTGGATC GAATCAGTAC 
CAGATCGATG CTGGTCAAAA GGCCTTCGGA GCACGGCAGT GCCAGCAGTG CGGATTGGTG 
TACACCGTGC ACGAGCCGGA GGAGGAGCTG CTTCATCGCG AGTACCACAA TTCCATCCAT 
GTGCTGCGCT TCAAGGGCTG GATCGACGAG GACATTGTGT CCGTTTATCC AGAATGGGCC 
AGCGACGGGC GCATCATACG GATCAACGAA CGCGCTCCGA CAGCTCGGCT CGACAGACTG 
CGGGATCTCA TTGGTGTGGT GGACAAAGAG CTGGGCTATT CCTCGTACAT TGTGCCAAAG 
ATCTTTGTAG CCTTCATAGC AGTGCGCAAG CAGCAGATCG TAGGCTTCTG CCTGGTGCAG 
CCCCTCTCGC AGGCCCATCG TTTCATCCAG GTCGATGGCA CGGACTACTT CAGCGAGGAA 
AGCTACCCGG CCAGCTGCGG CGTCTCACGC ATCTGGGTTT CTCCGCTGCA GCGACGCAGC 
GGGATCGCCA GCAAGCTGCT GCGAGTCGTC CAGTGCCACA CGGTGCTCGG TCAAGAGATC 
GCTAGGGAGT GCATTGCCTT CAGTACGCCC ACCGACGATG GTCGGGCTCT GGCACGCCAG 
TTCACCGGGT TGGATAACTT CCTCACCTAC GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eco ( NM_168201.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729236.2 Drosophila melanogaster establishment of cohesion, transcript variant B (eco), mRNA.

Price & Availability↑

CloneID	OFa20666
Clone ID Related Accession (Same CDS sequence)	NM_139849.3 , NM_168201.2
Accession Version	NM_168201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	establishment of cohesion, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 27, 2006 this sequence version replaced NM_168201.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGACCC CGACGGGTAG TGGGCGTCCC AGTCGCATGG CCACTCCGCG CCTTTCGGAG 
CGCAAGCGGC AACTGTTTGG CAGTCCCAGA TCCAGGCTGC GCCAAATCAA CGACGACGAG 
GATGATGCGG ACGTAGACAG TCTGGGTGTC TTGCCATTAA AGACCCATGT GGCGGCCAAC 
AGGAAGGGCA GGAGTCTCTT CGCCGCAGTC CCCGGCAAGT CAAGCAGCAG TGCGAACAGC 
AGCCCGGAAA CCAACAAGGA GAACAAAAAA ACCCGTGGCG GAGTCATGAC TGCCACGGCG 
GAGCAGCTGC CCCAATTGTT TACAGCCACC ATGCGGCTAA ATAGCAACAG CAGCAGCAAT 
AGTCGCAACA GCAGCCCCCG TCAGACGAGG GTCCAAAGAA AGCGAGCGGA TTCTTCAATG 
TCGTCGCCCA CCTCTTCCTC TGAAGGAACT CCTTCTTCCA GAGCCAGAAA TAGCATCAGA 
CGCAGTCCAC GAACCTTCAG TGCGCAAAAG GATCCAGATG CGTTCTCCTC GCCAGAATCT 
TTTCAGACTA GGCTAAGCAA GGTGGCAGCT ATGCTAATGA AGGGTCAGGA TTCAAGATCA 
ATGTTAGAAA AATCAAAGAA GAAACACAAC CACAGCCTAA AGACCACTGC CCAAGTGCAT 
ACAACTAAAC CGAAGAAAAC GAGCCCAGCT GAAGAGTCCC AGTCGGACGA CGAGAAACCT 
TCTAGCAGTA AGAACTCCAG GAAGAATACA GAAGTTCGTG AAACAAGGAG CTCGCAAATC 
ATCAGTCCAA AAACCAGGAA TAGGCGAAGA CCCTTCACGT CGGCGGATAT AAACTGCAAG 
ACGCTGAAGG CTGCTGCCCA TCTACATGAG AACATGAGGT CGTATGATGA AGAAAAAACA 
GCAGCAGTTA AGCTAGAAAA CAGCAGAAGC AGATCCAAGT CCCCGGTAGA AGTGTTCAAG 
TCAAATGACG ATGCAGTAAA ACGAAACACA GGAAACACCA ACAATAAAAC AGCGAAATCT 
TCCGAAGTTG CCACAGCAAA AAGGCCAGAA AGCCCAGGAA GTAGTATGAA GATTGATGTC 
GAAGTGCCCG AATCGGACGA AGAAGCATCC AATCATAAGC CTCAAAAGCG ACAGCACCCA 
GAAACGTCCA CCCCGGTTGC TCCTTCAGCG GACGCCGACT CTGGTTCGCC GCAGAGCAAG 
ATGCGCAAGG TGACGCTGAG CAGCAGCATT CCCACCATGG CCTTCTATTC CCACAGTGGA 
GAAGCGGTCA CAAAGTCAAG AAGGCGGCCG TCCATATCCA AGAATTCGCT TAAACAACCA 
ACGAAAATCT CGCCAACCTC CCGTCCACTA TTGGGCATTA ATAAGGGAGT TCACCACAAA 
ATACGCAAGC GTCACGGTTT TGCCAACCGA CTGCCAGCCA CCGATATGGA CAACATTCTC 
AACTCGCTGA GCAACGAACG GCTCAAGAAC CTGATCACCA CCAAGCGTGA AGAGCGCGCC 
AAAGTGGAGG AAGTCCACCA GATTCTCCGT AATGCCAAGG ACCCGATCAA AATGGCCAAA 
CCATTGAGTG TGATTGAAGC CGACGATGCT AACAACAATA ACAATCTGCC AGCAACCGCA 
TGGCAAGAAA CTTCCGCCGA TTTCTCCGAT CTTAGTGATG TCGAAGACAT TGATCCCATC 
ATCGAGGTGG AGCCTATCAT ACCAATAATT CGCCACGAGC CGGTCCAGAA ATCCCCAACC 
GCCGAGCCAG CCGATCTAAG CAAACGAAAG TTCTTCAAAT CGGGCCGCCG TAGTAGCACC 
TGCATGGAGG TGAGAATCAC GGACAACATC CGGGCCAGCG TCAGCCAGGG CAAGATTGAA 
CTCGTCCAAA CGATACGCCG TAAGCCGCGG CAAGTGCGCG TCAAGTCGGC CACGATATTT 
TCCGCCGAGC AGGCGACTGT GGATGCGATC CTAAAGAATC TGGACGATAC GGTGGTTGAT 
GAGATTGTAG AAGCGAATCC GGTGGTACAG GCAACGCCCA TTGATGCGGA GGAGACGACC 
ATGGAAACGG AGTCGCTACC GGATATAATT GAGTATGCCC CAGAGGCGAA CGATGTGGAA 
ATTGATCCCT TTGCTGAGTT CCGTCAACGT TTGCCATACC AAACCGATGA TCCCAATGTC 
GTGGAGCAAC AGCAAATTTT ACTAGAGTTT CTCATTAGCA ACAATATTTG CACCGAAAAG 
AACTTTGAGA TATTTATTGC AAATCCGGAT GACTACAAGG ACGAGGCCAA TCAAATTGTG 
GACAATTTAT ACATGGTGGT GAACAGCGAG GAGGCTGCGC AGCTTGCTCA GATGGAAACA 
GTGGAAAATA CAGCGGTGGC CATTGCACCA AAACAGGATG CGCCAGCAGT AGAGGAAGTT 
CAGCCAAAGC TTTTCCCCAT ATTCACTCAA CGCTTACAGC CCGTTGTCCA GAAATCGTTG 
CGGCGTCGGC CGGATACATC GATGAGATTG CTAACGGCGG CGGGTGGATC GAATCAGTAC 
CAGATCGATG CTGGTCAAAA GGCCTTCGGA GCACGGCAGT GCCAGCAGTG CGGATTGGTG 
TACACCGTGC ACGAGCCGGA GGAGGAGCTG CTTCATCGCG AGTACCACAA TTCCATCCAT 
GTGCTGCGCT TCAAGGGCTG GATCGACGAG GACATTGTGT CCGTTTATCC AGAATGGGCC 
AGCGACGGGC GCATCATACG GATCAACGAA CGCGCTCCGA CAGCTCGGCT CGACAGACTG 
CGGGATCTCA TTGGTGTGGT GGACAAAGAG CTGGGCTATT CCTCGTACAT TGTGCCAAAG 
ATCTTTGTAG CCTTCATAGC AGTGCGCAAG CAGCAGATCG TAGGCTTCTG CCTGGTGCAG 
CCCCTCTCGC AGGCCCATCG TTTCATCCAG GTCGATGGCA CGGACTACTT CAGCGAGGAA 
AGCTACCCGG CCAGCTGCGG CGTCTCACGC ATCTGGGTTT CTCCGCTGCA GCGACGCAGC 
GGGATCGCCA GCAAGCTGCT GCGAGTCGTC CAGTGCCACA CGGTGCTCGG TCAAGAGATC 
GCTAGGGAGT GCATTGCCTT CAGTACGCCC ACCGACGATG GTCGGGCTCT GGCACGCCAG 
TTCACCGGGT TGGATAACTT CCTCACCTAC GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729236.2
CDS	98..3256
Translation	METPTGSGRPSRMATPRLSERKRQLFGSPRSRLRQINDDEDDADVDSLGVLPLKTHVAANRKGRSLFAAVPGKSSSSANSSPETNKENKKTRGGVMTATAEQLPQLFTATMRLNSNSSSNSRNSSPRQTRVQRKRADSSMSSPTSSSEGTPSSRARNSIRRSPRTFSAQKDPDAFSSPESFQTRLSKVAAMLMKGQDSRSMLEKSKKKHNHSLKTTAQVHTTKPKKTSPAEESQSDDEKPSSSKNSRKNTEVRETRSSQIISPKTRNRRRPFTSADINCKTLKAAAHLHENMRSYDEEKTAAVKLENSRSRSKSPVEVFKSNDDAVKRNTGNTNNKTAKSSEVATAKRPESPGSSMKIDVEVPESDEEASNHKPQKRQHPETSTPVAPSADADSGSPQSKMRKVTLSSSIPTMAFYSHSGEAVTKSRRRPSISKNSLKQPTKISPTSRPLLGINKGVHHKIRKRHGFANRLPATDMDNILNSLSNERLKNLITTKREERAKVEEVHQILRNAKDPIKMAKPLSVIEADDANNNNNLPATAWQETSADFSDLSDVEDIDPIIEVEPIIPIIRHEPVQKSPTAEPADLSKRKFFKSGRRSSTCMEVRITDNIRASVSQGKIELVQTIRRKPRQVRVKSATIFSAEQATVDAILKNLDDTVVDEIVEANPVVQATPIDAEETTMETESLPDIIEYAPEANDVEIDPFAEFRQRLPYQTDDPNVVEQQQILLEFLISNNICTEKNFEIFIANPDDYKDEANQIVDNLYMVVNSEEAAQLAQMETVENTAVAIAPKQDAPAVEEVQPKLFPIFTQRLQPVVQKSLRRRPDTSMRLLTAAGGSNQYQIDAGQKAFGARQCQQCGLVYTVHEPEEELLHREYHNSIHVLRFKGWIDEDIVSVYPEWASDGRIIRINERAPTARLDRLRDLIGVVDKELGYSSYIVPKIFVAFIAVRKQQIVGFCLVQPLSQAHRFIQVDGTDYFSEESYPASCGVSRIWVSPLQRRSGIASKLLRVVQCHTVLGQEIARECIAFSTPTDDGRALARQFTGLDNFLTYDQ

Target ORF information:

RefSeq Version	NM_168201.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster establishment of cohesion, transcript variant B (eco), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168201.2

ORF Insert Sequence:

ATGGAGACCC CGACGGGTAG TGGGCGTCCC AGTCGCATGG CCACTCCGCG CCTTTCGGAG 
CGCAAGCGGC AACTGTTTGG CAGTCCCAGA TCCAGGCTGC GCCAAATCAA CGACGACGAG 
GATGATGCGG ACGTAGACAG TCTGGGTGTC TTGCCATTAA AGACCCATGT GGCGGCCAAC 
AGGAAGGGCA GGAGTCTCTT CGCCGCAGTC CCCGGCAAGT CAAGCAGCAG TGCGAACAGC 
AGCCCGGAAA CCAACAAGGA GAACAAAAAA ACCCGTGGCG GAGTCATGAC TGCCACGGCG 
GAGCAGCTGC CCCAATTGTT TACAGCCACC ATGCGGCTAA ATAGCAACAG CAGCAGCAAT 
AGTCGCAACA GCAGCCCCCG TCAGACGAGG GTCCAAAGAA AGCGAGCGGA TTCTTCAATG 
TCGTCGCCCA CCTCTTCCTC TGAAGGAACT CCTTCTTCCA GAGCCAGAAA TAGCATCAGA 
CGCAGTCCAC GAACCTTCAG TGCGCAAAAG GATCCAGATG CGTTCTCCTC GCCAGAATCT 
TTTCAGACTA GGCTAAGCAA GGTGGCAGCT ATGCTAATGA AGGGTCAGGA TTCAAGATCA 
ATGTTAGAAA AATCAAAGAA GAAACACAAC CACAGCCTAA AGACCACTGC CCAAGTGCAT 
ACAACTAAAC CGAAGAAAAC GAGCCCAGCT GAAGAGTCCC AGTCGGACGA CGAGAAACCT 
TCTAGCAGTA AGAACTCCAG GAAGAATACA GAAGTTCGTG AAACAAGGAG CTCGCAAATC 
ATCAGTCCAA AAACCAGGAA TAGGCGAAGA CCCTTCACGT CGGCGGATAT AAACTGCAAG 
ACGCTGAAGG CTGCTGCCCA TCTACATGAG AACATGAGGT CGTATGATGA AGAAAAAACA 
GCAGCAGTTA AGCTAGAAAA CAGCAGAAGC AGATCCAAGT CCCCGGTAGA AGTGTTCAAG 
TCAAATGACG ATGCAGTAAA ACGAAACACA GGAAACACCA ACAATAAAAC AGCGAAATCT 
TCCGAAGTTG CCACAGCAAA AAGGCCAGAA AGCCCAGGAA GTAGTATGAA GATTGATGTC 
GAAGTGCCCG AATCGGACGA AGAAGCATCC AATCATAAGC CTCAAAAGCG ACAGCACCCA 
GAAACGTCCA CCCCGGTTGC TCCTTCAGCG GACGCCGACT CTGGTTCGCC GCAGAGCAAG 
ATGCGCAAGG TGACGCTGAG CAGCAGCATT CCCACCATGG CCTTCTATTC CCACAGTGGA 
GAAGCGGTCA CAAAGTCAAG AAGGCGGCCG TCCATATCCA AGAATTCGCT TAAACAACCA 
ACGAAAATCT CGCCAACCTC CCGTCCACTA TTGGGCATTA ATAAGGGAGT TCACCACAAA 
ATACGCAAGC GTCACGGTTT TGCCAACCGA CTGCCAGCCA CCGATATGGA CAACATTCTC 
AACTCGCTGA GCAACGAACG GCTCAAGAAC CTGATCACCA CCAAGCGTGA AGAGCGCGCC 
AAAGTGGAGG AAGTCCACCA GATTCTCCGT AATGCCAAGG ACCCGATCAA AATGGCCAAA 
CCATTGAGTG TGATTGAAGC CGACGATGCT AACAACAATA ACAATCTGCC AGCAACCGCA 
TGGCAAGAAA CTTCCGCCGA TTTCTCCGAT CTTAGTGATG TCGAAGACAT TGATCCCATC 
ATCGAGGTGG AGCCTATCAT ACCAATAATT CGCCACGAGC CGGTCCAGAA ATCCCCAACC 
GCCGAGCCAG CCGATCTAAG CAAACGAAAG TTCTTCAAAT CGGGCCGCCG TAGTAGCACC 
TGCATGGAGG TGAGAATCAC GGACAACATC CGGGCCAGCG TCAGCCAGGG CAAGATTGAA 
CTCGTCCAAA CGATACGCCG TAAGCCGCGG CAAGTGCGCG TCAAGTCGGC CACGATATTT 
TCCGCCGAGC AGGCGACTGT GGATGCGATC CTAAAGAATC TGGACGATAC GGTGGTTGAT 
GAGATTGTAG AAGCGAATCC GGTGGTACAG GCAACGCCCA TTGATGCGGA GGAGACGACC 
ATGGAAACGG AGTCGCTACC GGATATAATT GAGTATGCCC CAGAGGCGAA CGATGTGGAA 
ATTGATCCCT TTGCTGAGTT CCGTCAACGT TTGCCATACC AAACCGATGA TCCCAATGTC 
GTGGAGCAAC AGCAAATTTT ACTAGAGTTT CTCATTAGCA ACAATATTTG CACCGAAAAG 
AACTTTGAGA TATTTATTGC AAATCCGGAT GACTACAAGG ACGAGGCCAA TCAAATTGTG 
GACAATTTAT ACATGGTGGT GAACAGCGAG GAGGCTGCGC AGCTTGCTCA GATGGAAACA 
GTGGAAAATA CAGCGGTGGC CATTGCACCA AAACAGGATG CGCCAGCAGT AGAGGAAGTT 
CAGCCAAAGC TTTTCCCCAT ATTCACTCAA CGCTTACAGC CCGTTGTCCA GAAATCGTTG 
CGGCGTCGGC CGGATACATC GATGAGATTG CTAACGGCGG CGGGTGGATC GAATCAGTAC 
CAGATCGATG CTGGTCAAAA GGCCTTCGGA GCACGGCAGT GCCAGCAGTG CGGATTGGTG 
TACACCGTGC ACGAGCCGGA GGAGGAGCTG CTTCATCGCG AGTACCACAA TTCCATCCAT 
GTGCTGCGCT TCAAGGGCTG GATCGACGAG GACATTGTGT CCGTTTATCC AGAATGGGCC 
AGCGACGGGC GCATCATACG GATCAACGAA CGCGCTCCGA CAGCTCGGCT CGACAGACTG 
CGGGATCTCA TTGGTGTGGT GGACAAAGAG CTGGGCTATT CCTCGTACAT TGTGCCAAAG 
ATCTTTGTAG CCTTCATAGC AGTGCGCAAG CAGCAGATCG TAGGCTTCTG CCTGGTGCAG 
CCCCTCTCGC AGGCCCATCG TTTCATCCAG GTCGATGGCA CGGACTACTT CAGCGAGGAA 
AGCTACCCGG CCAGCTGCGG CGTCTCACGC ATCTGGGTTT CTCCGCTGCA GCGACGCAGC 
GGGATCGCCA GCAAGCTGCT GCGAGTCGTC CAGTGCCACA CGGTGCTCGG TCAAGAGATC 
GCTAGGGAGT GCATTGCCTT CAGTACGCCC ACCGACGATG GTCGGGCTCT GGCACGCCAG 
TTCACCGGGT TGGATAACTT CCTCACCTAC GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eco cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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