CG10274 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG10274
Entrez Gene ID	38716
Full Name	CG10274 gene product from transcript CG10274-RB
Synonyms	Dmel\CG10274
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65A7-65A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104055.2	NP_001097525.1	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG10147	NP_001261477.1
Drosophila melanogaster (fruit fly)	D19A	NP_477304.1
Drosophila melanogaster (fruit fly)	CG10274	NP_001097525.1
Drosophila melanogaster (fruit fly)	CG7386	NP_648036.1
Drosophila melanogaster (fruit fly)	D19B	NP_477297.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010882	XP_309819.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG10274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG10274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17687	NM_001104055.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG10274), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG10274 ( NM_001104055.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097525.1 Drosophila melanogaster uncharacterized protein (CG10274), mRNA.

Price & Availability↑

CloneID	OFa17687
Clone ID Related Accession (Same CDS sequence)	NM_001104055.2
Accession Version	NM_001104055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104055.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGAGG AGGCCGAGGC AGCCCAGTAC TCGGTGCACG CGGTGTGCCG CATCTGCCTG 
AATCGCCTGC CGGAGAATGG CGGCGGCGCA GGATCCTTTG ACCTGTTCCT GATACCCGGA 
TTGGCCAAGA AACTGTGTGT CTGCACTTCG CTGGCCGTGG AGCAGTCGGA TGGCTTCCCC 
AAGTGTCTGT GCGCGCAATG CTTCAGCCGG CTGGACGATA TGCACGAGTT CCAAAAGCTG 
TGCGTGGACT CGGTGCAAAA GTTCCAGGAC ATGGTGGCCA GGAACGTCTT TTGCCCGCCA 
GCGAGTGCGC AGATGAAAAA CTTTGATTTG CTCAACGTGG CGGGGAACGA GCCTGAGCTG 
GTGGGGCAGG ACGAGGAGGA TCGTATCAAC TTCGATCCGC TACTGGATCA CAAAATGGAG 
ATGATCGAGA ACGAGGAGGA TGTGTTCAAG ATGTTGGAGC ACGTGGACAA GGAGGCCGAG 
CAGGTGGAGA AGGAAGTGAA GCAAGATGAA TTGGACGCCG CCGATCTGAT GACCATCTTT 
GCCGCGGAAT CCTCGGAGGA GAGCGAAGAA GATATTGACC AAGATGTGGA CTTTGAGCCC 
AACAGTAGCG ATGGTGACGA TGATGTGCCT TTGGCCCAGC GCTTACGGGA TAATTCGCGG 
ATGATACCAA AAGCAAAGGC AGCCGTAATT AAGGAAGAGG ACCAAGAGTT CCCAACAGGC 
TCCGAAGAGG AGGACGAGGA CGGAGAAAAG TCAAAGAGTC GACGCAAGAG GATTCCGCCT 
GGCGAACGGC ACCTGCACCG CATCATTGAC TGCCACATTT GTCACCAAAA GTTCAAGAAG 
GCCATCCGCT ACGAAGAGCA CATGAAGCAC CACAACGACC TGCTGCCCTT CCAGTGCAAA 
GTGGAAACCT GCAGGAAAGG TTTCACCACC GCTGGAGGAT TACGACTTCA CATCGACCAC 
GCCCACACGG AGCTATCCGA GGTGCACTCC TGCAATGTCG ATGGCTGCGG CAAGACCTTT 
CCGCGCATAC GCCTGCTCAC CTTTCACATG AAGAAGATGC ACGGCATCAC CAAGGCAGCG 
GCGCCCCCAC GGGATTACCC TTGCACCGAG TGCGAGAAGG TATTCCGCTG TCCCATGGCG 
CTCAAGAAGC ACATGTACAA GCACGACGGC AAGGAGTTGC CTTTCCCCTG TAACATCTGT 
GGCAAAAGGT TCGTAATCAA TAGTGCTCTA AAGGATCATC TGATGCGGCA TGCGGGCATT 
AAGAACTACG TGTGTCCGTA CTGTGGCGTG GGAAAAACCA CCAGGCAGGA ATGGAACACA 
CACATACTGA CGCATACCCA GGAGAAGAAG TTCAAGTGCC ACATATGCGA GCACGCATCG 
CACAATAAGC AGAGCCTGGC CAACCACATT AAGATCGTGC ACGAGAAGAT CAAAAACTAT 
GCGTGTCAAT ACTGTGGAAA GACCTTTGGC AAGTCACACG CCTGCAAGAT CCACGAGATG 
ACGCACACGG GGGAAAAGCG CTGTGAGTGC AAGGTTTGTG GAAAGAAGTT TCTCTATCCT 
AAGAGTCTGA CCAAGCACTT GAAGACTCAT GAGAAGCGTG TGCTTCGTGC TATTGAGACC 
TACCGCCAGC GGCAGGTGGA AATGGGCGAG ACGCCTGGTG AACAGTTCGA CAATCCACCC 
GCGCCACCAG TTGAGGGCAT ATCCATTGAG CCGATCATGT CCCGAAACGC TGCCGACGAA 
TTGCTTAAGG TGTGCGCCGA ATCCGTGGCC ACCATTCCCA AAGATCCACG CCGTGTACAA 
CGAGTGGATC TGGCGCAGTT GGCTGGCACT GCTGTGAACC CCATACCCTC TGTTTCAGTG 
CCGTCGTGGT CGCCGCAGGT GAACTTTACC AAGAAGGAGG GCAAACATAT CTGCCCCGGT 
TGTGGTCAGG GATTCAACAA TATCGGAAAC ATGAAGCGGC ACTATAAGAT CATCCACGAA 
AAGGTCAAAG ATTTTGCCTG CCGCTTTTGC CCCAAAAGAT TCGCAAAGGC TCAAACGTTG 
AGGCATCACG AGTGGATTCA TACGGGCGAG AAACCCTTTG AGTGCAAGAC CTGCGGCACC 
CATTTCCGCC AGGAGACGGC GCTGAAGCGC CACCAGCGCA CCCACGAGAA CCGTCCACCC 
GTCATTTCGC CCAAGTTTTA TGCCGCTCGC GAGGAGCTGG AAGAGCAGGA ACGCAGCAAG 
CGAGAGGCGA AGCGCCAGGC GGATGCGAAG CGGCGCGAAA TTGCGGAGGC GGCCAAGGAG 
CAGCTATCGT CGCTGCACAA GCTTGAAAGT AGTGACCGCA GCCTGCACTC CTACGATGAG 
TACCAGGAGG CAGCGGCGGA GCGAGCAGCG GCATCCGCTG AACTCCAGGC GCAGCAGTTT 
GAAGAGAACG AAGTAAAGCG ACTGGCGGAG GAGGAGCGCC GCAAGGTTCA GGAGGCTGCC 
TACGAGCAAC TGCAACTGTT GCAGCATCAG CAGGAGGTTG ACAAGGCGCA AAGCTCCTAC 
GATGGCTACT ACGCTCAAAA AGCACATGCC GATGGCACCA CTTTGGATGC TTTAAAAATC 
GATCATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097525.1
CDS	63..2654
Translation	MQEEAEAAQYSVHAVCRICLNRLPENGGGAGSFDLFLIPGLAKKLCVCTSLAVEQSDGFPKCLCAQCFSRLDDMHEFQKLCVDSVQKFQDMVARNVFCPPASAQMKNFDLLNVAGNEPELVGQDEEDRINFDPLLDHKMEMIENEEDVFKMLEHVDKEAEQVEKEVKQDELDAADLMTIFAAESSEESEEDIDQDVDFEPNSSDGDDDVPLAQRLRDNSRMIPKAKAAVIKEEDQEFPTGSEEEDEDGEKSKSRRKRIPPGERHLHRIIDCHICHQKFKKAIRYEEHMKHHNDLLPFQCKVETCRKGFTTAGGLRLHIDHAHTELSEVHSCNVDGCGKTFPRIRLLTFHMKKMHGITKAAAPPRDYPCTECEKVFRCPMALKKHMYKHDGKELPFPCNICGKRFVINSALKDHLMRHAGIKNYVCPYCGVGKTTRQEWNTHILTHTQEKKFKCHICEHASHNKQSLANHIKIVHEKIKNYACQYCGKTFGKSHACKIHEMTHTGEKRCECKVCGKKFLYPKSLTKHLKTHEKRVLRAIETYRQRQVEMGETPGEQFDNPPAPPVEGISIEPIMSRNAADELLKVCAESVATIPKDPRRVQRVDLAQLAGTAVNPIPSVSVPSWSPQVNFTKKEGKHICPGCGQGFNNIGNMKRHYKIIHEKVKDFACRFCPKRFAKAQTLRHHEWIHTGEKPFECKTCGTHFRQETALKRHQRTHENRPPVISPKFYAAREELEEQERSKREAKRQADAKRREIAEAAKEQLSSLHKLESSDRSLHSYDEYQEAAAERAAASAELQAQQFEENEVKRLAEEERRKVQEAAYEQLQLLQHQQEVDKAQSSYDGYYAQKAHADGTTLDALKIDHV

Target ORF information:

RefSeq Version	NM_001104055.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG10274), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104055.2

ORF Insert Sequence:

ATGCAGGAGG AGGCCGAGGC AGCCCAGTAC TCGGTGCACG CGGTGTGCCG CATCTGCCTG 
AATCGCCTGC CGGAGAATGG CGGCGGCGCA GGATCCTTTG ACCTGTTCCT GATACCCGGA 
TTGGCCAAGA AACTGTGTGT CTGCACTTCG CTGGCCGTGG AGCAGTCGGA TGGCTTCCCC 
AAGTGTCTGT GCGCGCAATG CTTCAGCCGG CTGGACGATA TGCACGAGTT CCAAAAGCTG 
TGCGTGGACT CGGTGCAAAA GTTCCAGGAC ATGGTGGCCA GGAACGTCTT TTGCCCGCCA 
GCGAGTGCGC AGATGAAAAA CTTTGATTTG CTCAACGTGG CGGGGAACGA GCCTGAGCTG 
GTGGGGCAGG ACGAGGAGGA TCGTATCAAC TTCGATCCGC TACTGGATCA CAAAATGGAG 
ATGATCGAGA ACGAGGAGGA TGTGTTCAAG ATGTTGGAGC ACGTGGACAA GGAGGCCGAG 
CAGGTGGAGA AGGAAGTGAA GCAAGATGAA TTGGACGCCG CCGATCTGAT GACCATCTTT 
GCCGCGGAAT CCTCGGAGGA GAGCGAAGAA GATATTGACC AAGATGTGGA CTTTGAGCCC 
AACAGTAGCG ATGGTGACGA TGATGTGCCT TTGGCCCAGC GCTTACGGGA TAATTCGCGG 
ATGATACCAA AAGCAAAGGC AGCCGTAATT AAGGAAGAGG ACCAAGAGTT CCCAACAGGC 
TCCGAAGAGG AGGACGAGGA CGGAGAAAAG TCAAAGAGTC GACGCAAGAG GATTCCGCCT 
GGCGAACGGC ACCTGCACCG CATCATTGAC TGCCACATTT GTCACCAAAA GTTCAAGAAG 
GCCATCCGCT ACGAAGAGCA CATGAAGCAC CACAACGACC TGCTGCCCTT CCAGTGCAAA 
GTGGAAACCT GCAGGAAAGG TTTCACCACC GCTGGAGGAT TACGACTTCA CATCGACCAC 
GCCCACACGG AGCTATCCGA GGTGCACTCC TGCAATGTCG ATGGCTGCGG CAAGACCTTT 
CCGCGCATAC GCCTGCTCAC CTTTCACATG AAGAAGATGC ACGGCATCAC CAAGGCAGCG 
GCGCCCCCAC GGGATTACCC TTGCACCGAG TGCGAGAAGG TATTCCGCTG TCCCATGGCG 
CTCAAGAAGC ACATGTACAA GCACGACGGC AAGGAGTTGC CTTTCCCCTG TAACATCTGT 
GGCAAAAGGT TCGTAATCAA TAGTGCTCTA AAGGATCATC TGATGCGGCA TGCGGGCATT 
AAGAACTACG TGTGTCCGTA CTGTGGCGTG GGAAAAACCA CCAGGCAGGA ATGGAACACA 
CACATACTGA CGCATACCCA GGAGAAGAAG TTCAAGTGCC ACATATGCGA GCACGCATCG 
CACAATAAGC AGAGCCTGGC CAACCACATT AAGATCGTGC ACGAGAAGAT CAAAAACTAT 
GCGTGTCAAT ACTGTGGAAA GACCTTTGGC AAGTCACACG CCTGCAAGAT CCACGAGATG 
ACGCACACGG GGGAAAAGCG CTGTGAGTGC AAGGTTTGTG GAAAGAAGTT TCTCTATCCT 
AAGAGTCTGA CCAAGCACTT GAAGACTCAT GAGAAGCGTG TGCTTCGTGC TATTGAGACC 
TACCGCCAGC GGCAGGTGGA AATGGGCGAG ACGCCTGGTG AACAGTTCGA CAATCCACCC 
GCGCCACCAG TTGAGGGCAT ATCCATTGAG CCGATCATGT CCCGAAACGC TGCCGACGAA 
TTGCTTAAGG TGTGCGCCGA ATCCGTGGCC ACCATTCCCA AAGATCCACG CCGTGTACAA 
CGAGTGGATC TGGCGCAGTT GGCTGGCACT GCTGTGAACC CCATACCCTC TGTTTCAGTG 
CCGTCGTGGT CGCCGCAGGT GAACTTTACC AAGAAGGAGG GCAAACATAT CTGCCCCGGT 
TGTGGTCAGG GATTCAACAA TATCGGAAAC ATGAAGCGGC ACTATAAGAT CATCCACGAA 
AAGGTCAAAG ATTTTGCCTG CCGCTTTTGC CCCAAAAGAT TCGCAAAGGC TCAAACGTTG 
AGGCATCACG AGTGGATTCA TACGGGCGAG AAACCCTTTG AGTGCAAGAC CTGCGGCACC 
CATTTCCGCC AGGAGACGGC GCTGAAGCGC CACCAGCGCA CCCACGAGAA CCGTCCACCC 
GTCATTTCGC CCAAGTTTTA TGCCGCTCGC GAGGAGCTGG AAGAGCAGGA ACGCAGCAAG 
CGAGAGGCGA AGCGCCAGGC GGATGCGAAG CGGCGCGAAA TTGCGGAGGC GGCCAAGGAG 
CAGCTATCGT CGCTGCACAA GCTTGAAAGT AGTGACCGCA GCCTGCACTC CTACGATGAG 
TACCAGGAGG CAGCGGCGGA GCGAGCAGCG GCATCCGCTG AACTCCAGGC GCAGCAGTTT 
GAAGAGAACG AAGTAAAGCG ACTGGCGGAG GAGGAGCGCC GCAAGGTTCA GGAGGCTGCC 
TACGAGCAAC TGCAACTGTT GCAGCATCAG CAGGAGGTTG ACAAGGCGCA AAGCTCCTAC 
GATGGCTACT ACGCTCAAAA AGCACATGCC GATGGCACCA CTTTGGATGC TTTAAAAATC 
GATCATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG10274 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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