dos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dos
Entrez Gene ID	38321
Full Name	daughter of sevenless
Synonyms	141511_at,CG1044,DOS,Dmel\CG1044,Dos,E(csw)3A,E(csw[CS])3A,EC3-4,Gab-1,Su(sev)3A,Su(sev[S11])3A
General protein information	Preferred Names daughter of sevenless Names CG1044 gene product from transcript CG1044-RB CG1044-PA CG1044-PB dos-PA dos-PB enhancer of corkscrew-CS suppressor of sevenless-S11
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62E7-62E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_079166.3	NP_523890.2	daughter of sevenless, isoform A
NM_167956.2	NP_728757.1	daughter of sevenless, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	dos	NP_523890.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005826	XP_315852.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identifies DRK/DOS/SOS as the upstream Rac GEF complex required for glial responses to axonal injury, and demonstrates a critical requirement for multiple GEFs in efficient glial activation after injury and internalization/degradation of axonal debris
Title: DRK/DOS/SOS converge with Crk/Mbc/dCed-12 to activate Rac1 during glial engulfment of axonal debris.

Signalling from the pTyr (YB) Neu (ErbB-2) autophosphorylation site was affected by a haplosufficiency in drk (Grb-2), and in genes thought to function downstream of Grb-2: dab, sos, csw (Shp-2), and dos (Gab-1).
Title: Genetic identification of effectors downstream of Neu (ErbB-2) autophosphorylation sites in a Drosophila model.

SH3 domain-mediated binding of the Drk protein to Dos is an important step in signaling of Drosophila receptor tyrosine kinases.
Title: SH3 domain-mediated binding of the Drk protein to Dos is an important step in signaling of Drosophila receptor tyrosine kinases.

The following dos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17306	NM_167956.2 Latest version!	Drosophila melanogaster daughter of sevenless (dos), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17307	NM_079166.3 Latest version!	Drosophila melanogaster daughter of sevenless (dos), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dos ( NM_167956.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728757.1 Drosophila melanogaster daughter of sevenless (dos), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17306
Clone ID Related Accession (Same CDS sequence)	NM_167956.2
Accession Version	NM_167956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughter of sevenless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167956.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGATA TCAAGACGCC CAAGCGCACC TACTACCTGG CTGCTGAGAC GGAGGCGGAT 
ATGCGCGACT GGGTGAACTG CATCTGCCAG GTGTGTCACC TACACGACAC AAAGCAATCG 
AATGAGTTGC CATTAGGAGC TGTGGGTGCA GACGAAAACC GCACCCAACA CACGAGCTCC 
AGTGGCGGTC TAAGCAACTC CACTCAAAAT ACGACCACCA CATCGTTGCA CTCGTCGGCC 
GGAACCACAG CGCCTCAGGC TTCGGTCCCA AATGCTGGAG GATCTGCCCA GCTGCGACGA 
CCGGCGGTCA TTGAAGAGCA ACCGATGCCC TCGAATGCCG GCAACAATAA CTCAGACTCG 
GTGTATGTCA ACACGGAGTA CAGCAATCGC GAGACGATGC TCTGCGATGC CAATTTCGAT 
CAGCAGGAAC TGCTCTCGGC TGCCCAGCAG CAACCGCCTC CATCGCCGGC CACGGCTCTT 
TATCTAAACC ACAGTGCTTT GATCCAAGCT CAGGCGGCAG CTGCAGCCGC CGAGCAACTG 
CAGCAGCAGC AGCAACAAGC TGCTCGCTTG GCGGTCAGTG CCAACGGAGT AGTGCGCAAA 
TTGCCGGAGC ATCTGGTGCT CACGCAGCAG ACCCTGGCGG AGGCAGCTGC CCAGCAACAC 
AGCAGCGTAC AGGCTTCACC CGCTTTGAGC ACCGCATCCG GACCGTACAT ACCCATCAGC 
GAGTGCTTCT CGGGCAGCCC GAGATTCCTG CCCGGCGTTC CACTGCCCGG CGCAGATTTG 
GCCATACCAA ACAACCCCAC CACGCCGTTG AACAACTTGG ATCCCAAGTT CTACGATACG 
CCGCGTAGTC ATAATAACAT AGGTCTAAAT CTGACCAACG ATCAATCCTA CTCACCGAAG 
ATAACCAATT TGAGTCTGCA ACAATTGGCC AATAACAATG CTAGTAAACA ACGCTCCGAC 
TCCGATTCGG AAAGCGTTTT CACCGATGAC GATGAGTGGG CTCATCCTTT GCCACTGCGC 
GAGAATGTGG ATCGCAGTAC CCGACCTTCG GATAGTTCCA TTGAAAACGA ATCATTCGTG 
CTAACGTACT CGCAGCGATT CTCTAAAATG CCCGAGGAAG GAGGAGCGAT CGTGCCACCA 
GCTGAAAAGA GTTCCAAGCT AGCAGGAGCA GCATCTCTGC CAGAGGCTGG TGACCAGGGA 
ACGCTGGATA AGCTGGCCAA GGTGCTGAAG AACAAAAATA ATCTCATATT GGACTTTAAG 
GAGAATGAAA AAATTCCTCG CGATCTGCCG CAGCTGTCGG ATACGGAAAA TACTTCGCCG 
GCCATTGTGG CCCGCCGCAA CGCGCACTCC GCTTTCATCG AGGAAAGCTA TGACATTCCA 
AGGTCCCACC AGCAGCCTTA CTACAACGTC AACCAGCTGC TGGGCGAGCG ACCAGTCACC 
AGCCCGCACA ATTCCAATCC CATTGCAGCA TCCACACCGA ATCTCATGGC GGCCGATCTG 
GGTGCAGTGG CCGCAATTTC AGCGGCAGCG AATCCCGGCC TGATGGGTGA GGCACAGGCA 
GTCGCATCAT CGCCCACCAG TGCGCGCACA TTGCCTCGAC ACTGCTACAC CAATGCAGCT 
CCCACAAAGA TGGAGGGCAA TGTTTTTCGA TACGACTTCA TGGAGCAGGC GGACTGCCCG 
CCGGTTAACC GGAAACTTAA GCCAAAGGTG GCGGGTGGAT TGCCAGTTGT AGAGGACAAA 
CCGCCGGAGG AGTTTCCTGC AAAGCCCCCG GTGGGCGTAG ATCAGTTAAC CAATAAACTG 
GGTGCAGCCC AACTGCAGCA GCCCATTGGA CCGCCCAGTG TGGACAGGAA GTGCAAACCG 
AATGCCTATA AGTTGGGCAA TTCGGCTACC ATGTCGCCGG CAACGCGACG TTCCTCCGGT 
GCCCCTCTCT CAATGGTGCT GCCGCATGAA ACGGATGTAC ACTCCCCGGC GGCAGCCAAC 
GCCTTCTTTC ACGAGACCCG CACCCTGCCT CGCCAACAGC ATCGGCATCA TCCGAACAGT 
CCCGGCAGCA TGTCGGTACA ACATCAGCGC ACCGCCTCCG CAGCGGCTGC CATGATGAGC 
TTGACGGCAG CAGCTGCTCC CAAGCAGCAA GCGGCGGCGC AGACTGAGCA CAAGCTGCAG 
TACTTTGATC TGGATGTGAC CAACAAACCG CCATTGCTAA ATCGATCCAG CATGAGTGTG 
GGGAATCTGT ACTCACAAGG CGGAAATGGA GCTAGTGGAA TGCGATTCGC AGGCGTCGAA 
GCTGGCGGAG CTCGAGCGCC AGTGCCTAGC AGCGTGGTCT ATAGATCCGT GGACTTTGTC 
AAGACGGAGG CATTTAAGAG GATTCGAGAG GAGCGCGAGA GCAGCGGCAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728757.1
CDS	1726..4122
Translation	MFDIKTPKRTYYLAAETEADMRDWVNCICQVCHLHDTKQSNELPLGAVGADENRTQHTSSSGGLSNSTQNTTTTSLHSSAGTTAPQASVPNAGGSAQLRRPAVIEEQPMPSNAGNNNSDSVYVNTEYSNRETMLCDANFDQQELLSAAQQQPPPSPATALYLNHSALIQAQAAAAAAEQLQQQQQQAARLAVSANGVVRKLPEHLVLTQQTLAEAAAQQHSSVQASPALSTASGPYIPISECFSGSPRFLPGVPLPGADLAIPNNPTTPLNNLDPKFYDTPRSHNNIGLNLTNDQSYSPKITNLSLQQLANNNASKQRSDSDSESVFTDDDEWAHPLPLRENVDRSTRPSDSSIENESFVLTYSQRFSKMPEEGGAIVPPAEKSSKLAGAASLPEAGDQGTLDKLAKVLKNKNNLILDFKENEKIPRDLPQLSDTENTSPAIVARRNAHSAFIEESYDIPRSHQQPYYNVNQLLGERPVTSPHNSNPIAASTPNLMAADLGAVAAISAAANPGLMGEAQAVASSPTSARTLPRHCYTNAAPTKMEGNVFRYDFMEQADCPPVNRKLKPKVAGGLPVVEDKPPEEFPAKPPVGVDQLTNKLGAAQLQQPIGPPSVDRKCKPNAYKLGNSATMSPATRRSSGAPLSMVLPHETDVHSPAAANAFFHETRTLPRQQHRHHPNSPGSMSVQHQRTASAAAAMMSLTAAAAPKQQAAAQTEHKLQYFDLDVTNKPPLLNRSSMSVGNLYSQGGNGASGMRFAGVEAGGARAPVPSSVVYRSVDFVKTEAFKRIREERESSGNK

Target ORF information:

RefSeq Version	NM_167956.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughter of sevenless (dos), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167956.2

ORF Insert Sequence:

ATGTTCGATA TCAAGACGCC CAAGCGCACC TACTACCTGG CTGCTGAGAC GGAGGCGGAT 
ATGCGCGACT GGGTGAACTG CATCTGCCAG GTGTGTCACC TACACGACAC AAAGCAATCG 
AATGAGTTGC CATTAGGAGC TGTGGGTGCA GACGAAAACC GCACCCAACA CACGAGCTCC 
AGTGGCGGTC TAAGCAACTC CACTCAAAAT ACGACCACCA CATCGTTGCA CTCGTCGGCC 
GGAACCACAG CGCCTCAGGC TTCGGTCCCA AATGCTGGAG GATCTGCCCA GCTGCGACGA 
CCGGCGGTCA TTGAAGAGCA ACCGATGCCC TCGAATGCCG GCAACAATAA CTCAGACTCG 
GTGTATGTCA ACACGGAGTA CAGCAATCGC GAGACGATGC TCTGCGATGC CAATTTCGAT 
CAGCAGGAAC TGCTCTCGGC TGCCCAGCAG CAACCGCCTC CATCGCCGGC CACGGCTCTT 
TATCTAAACC ACAGTGCTTT GATCCAAGCT CAGGCGGCAG CTGCAGCCGC CGAGCAACTG 
CAGCAGCAGC AGCAACAAGC TGCTCGCTTG GCGGTCAGTG CCAACGGAGT AGTGCGCAAA 
TTGCCGGAGC ATCTGGTGCT CACGCAGCAG ACCCTGGCGG AGGCAGCTGC CCAGCAACAC 
AGCAGCGTAC AGGCTTCACC CGCTTTGAGC ACCGCATCCG GACCGTACAT ACCCATCAGC 
GAGTGCTTCT CGGGCAGCCC GAGATTCCTG CCCGGCGTTC CACTGCCCGG CGCAGATTTG 
GCCATACCAA ACAACCCCAC CACGCCGTTG AACAACTTGG ATCCCAAGTT CTACGATACG 
CCGCGTAGTC ATAATAACAT AGGTCTAAAT CTGACCAACG ATCAATCCTA CTCACCGAAG 
ATAACCAATT TGAGTCTGCA ACAATTGGCC AATAACAATG CTAGTAAACA ACGCTCCGAC 
TCCGATTCGG AAAGCGTTTT CACCGATGAC GATGAGTGGG CTCATCCTTT GCCACTGCGC 
GAGAATGTGG ATCGCAGTAC CCGACCTTCG GATAGTTCCA TTGAAAACGA ATCATTCGTG 
CTAACGTACT CGCAGCGATT CTCTAAAATG CCCGAGGAAG GAGGAGCGAT CGTGCCACCA 
GCTGAAAAGA GTTCCAAGCT AGCAGGAGCA GCATCTCTGC CAGAGGCTGG TGACCAGGGA 
ACGCTGGATA AGCTGGCCAA GGTGCTGAAG AACAAAAATA ATCTCATATT GGACTTTAAG 
GAGAATGAAA AAATTCCTCG CGATCTGCCG CAGCTGTCGG ATACGGAAAA TACTTCGCCG 
GCCATTGTGG CCCGCCGCAA CGCGCACTCC GCTTTCATCG AGGAAAGCTA TGACATTCCA 
AGGTCCCACC AGCAGCCTTA CTACAACGTC AACCAGCTGC TGGGCGAGCG ACCAGTCACC 
AGCCCGCACA ATTCCAATCC CATTGCAGCA TCCACACCGA ATCTCATGGC GGCCGATCTG 
GGTGCAGTGG CCGCAATTTC AGCGGCAGCG AATCCCGGCC TGATGGGTGA GGCACAGGCA 
GTCGCATCAT CGCCCACCAG TGCGCGCACA TTGCCTCGAC ACTGCTACAC CAATGCAGCT 
CCCACAAAGA TGGAGGGCAA TGTTTTTCGA TACGACTTCA TGGAGCAGGC GGACTGCCCG 
CCGGTTAACC GGAAACTTAA GCCAAAGGTG GCGGGTGGAT TGCCAGTTGT AGAGGACAAA 
CCGCCGGAGG AGTTTCCTGC AAAGCCCCCG GTGGGCGTAG ATCAGTTAAC CAATAAACTG 
GGTGCAGCCC AACTGCAGCA GCCCATTGGA CCGCCCAGTG TGGACAGGAA GTGCAAACCG 
AATGCCTATA AGTTGGGCAA TTCGGCTACC ATGTCGCCGG CAACGCGACG TTCCTCCGGT 
GCCCCTCTCT CAATGGTGCT GCCGCATGAA ACGGATGTAC ACTCCCCGGC GGCAGCCAAC 
GCCTTCTTTC ACGAGACCCG CACCCTGCCT CGCCAACAGC ATCGGCATCA TCCGAACAGT 
CCCGGCAGCA TGTCGGTACA ACATCAGCGC ACCGCCTCCG CAGCGGCTGC CATGATGAGC 
TTGACGGCAG CAGCTGCTCC CAAGCAGCAA GCGGCGGCGC AGACTGAGCA CAAGCTGCAG 
TACTTTGATC TGGATGTGAC CAACAAACCG CCATTGCTAA ATCGATCCAG CATGAGTGTG 
GGGAATCTGT ACTCACAAGG CGGAAATGGA GCTAGTGGAA TGCGATTCGC AGGCGTCGAA 
GCTGGCGGAG CTCGAGCGCC AGTGCCTAGC AGCGTGGTCT ATAGATCCGT GGACTTTGTC 
AAGACGGAGG CATTTAAGAG GATTCGAGAG GAGCGCGAGA GCAGCGGCAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dos ( NM_079166.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523890.2 Drosophila melanogaster daughter of sevenless (dos), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17307
Clone ID Related Accession (Same CDS sequence)	NM_079166.3
Accession Version	NM_079166.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughter of sevenless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079166.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGCA CTTTCTACGA GGGCTGGCTA ATTAAGTCGC CGCCCACCAA GCGCATTTGG 
CGAGCACGCT GGAGGCGTCG CTACTTCACG CTGAAGCAGG GCGAGATACC GGAGCAGTTC 
TGCCTCGAAT ACTACACCGA CCACAACTGC CGCAAGCTGA AGGGCGTTAT CGATTTGGAT 
CAGTGCGAGC AGGTGGACTG TGGCCTGCGG CTGGAGAATC GCAAGCAGAA GTTTCAGTAC 
ATGTTCGATA TCAAGACGCC CAAGCGCACC TACTACCTGG CTGCTGAGAC GGAGGCGGAT 
ATGCGCGACT GGGTGAACTG CATCTGCCAG GTGTGTCACC TACACGACAC AAAGCAATCG 
AATGAGTTGC CATTAGGAGC TGTGGGTGCA GACGAAAACC GCACCCAACA CACGAGCTCC 
AGTGGCGGTC TAAGCAACTC CACTCAAAAT ACGACCACCA CATCGTTGCA CTCGTCGGCC 
GGAACCACAG CGCCTCAGGC TTCGGTCCCA AATGCTGGAG GATCTGCCCA GCTGCGACGA 
CCGGCGGTCA TTGAAGAGCA ACCGATGCCC TCGAATGCCG GCAACAATAA CTCAGACTCG 
GTGTATGTCA ACACGGAGTA CAGCAATCGC GAGACGATGC TCTGCGATGC CAATTTCGAT 
CAGCAGGAAC TGCTCTCGGC TGCCCAGCAG CAACCGCCTC CATCGCCGGC CACGGCTCTT 
TATCTAAACC ACAGTGCTTT GATCCAAGCT CAGGCGGCAG CTGCAGCCGC CGAGCAACTG 
CAGCAGCAGC AGCAACAAGC TGCTCGCTTG GCGGTCAGTG CCAACGGAGT AGTGCGCAAA 
TTGCCGGAGC ATCTGGTGCT CACGCAGCAG ACCCTGGCGG AGGCAGCTGC CCAGCAACAC 
AGCAGCGTAC AGGCTTCACC CGCTTTGAGC ACCGCATCCG GACCGTACAT ACCCATCAGC 
GAGTGCTTCT CGGGCAGCCC GAGATTCCTG CCCGGCGTTC CACTGCCCGG CGCAGATTTG 
GCCATACCAA ACAACCCCAC CACGCCGTTG AACAACTTGG ATCCCAAGTT CTACGATACG 
CCGCGTAGTC ATAATAACAT AGGTCTAAAT CTGACCAACG ATCAATCCTA CTCACCGAAG 
ATAACCAATT TGAGTCTGCA ACAATTGGCC AATAACAATG CTAGTAAACA ACGCTCCGAC 
TCCGATTCGG AAAGCGTTTT CACCGATGAC GATGAGTGGG CTCATCCTTT GCCACTGCGC 
GAGAATGTGG ATCGCAGTAC CCGACCTTCG GATAGTTCCA TTGAAAACGA ATCATTCGTG 
CTAACGTACT CGCAGCGATT CTCTAAAATG CCCGAGGAAG GAGGAGCGAT CGTGCCACCA 
GCTGAAAAGA GTTCCAAGCT AGCAGGAGCA GCATCTCTGC CAGAGGCTGG TGACCAGGGA 
ACGCTGGATA AGCTGGCCAA GGTGCTGAAG AACAAAAATA ATCTCATATT GGACTTTAAG 
GAGAATGAAA AAATTCCTCG CGATCTGCCG CAGCTGTCGG ATACGGAAAA TACTTCGCCG 
GCCATTGTGG CCCGCCGCAA CGCGCACTCC GCTTTCATCG AGGAAAGCTA TGACATTCCA 
AGGTCCCACC AGCAGCCTTA CTACAACGTC AACCAGCTGC TGGGCGAGCG ACCAGTCACC 
AGCCCGCACA ATTCCAATCC CATTGCAGCA TCCACACCGA ATCTCATGGC GGCCGATCTG 
GGTGCAGTGG CCGCAATTTC AGCGGCAGCG AATCCCGGCC TGATGGGTGA GGCACAGGCA 
GTCGCATCAT CGCCCACCAG TGCGCGCACA TTGCCTCGAC ACTGCTACAC CAATGCAGCT 
CCCACAAAGA TGGAGGGCAA TGTTTTTCGA TACGACTTCA TGGAGCAGGC GGACTGCCCG 
CCGGTTAACC GGAAACTTAA GCCAAAGGTG GCGGGTGGAT TGCCAGTTGT AGAGGACAAA 
CCGCCGGAGG AGTTTCCTGC AAAGCCCCCG GTGGGCGTAG ATCAGTTAAC CAATAAACTG 
GGTGCAGCCC AACTGCAGCA GCCCATTGGA CCGCCCAGTG TGGACAGGAA GTGCAAACCG 
AATGCCTATA AGTTGGGCAA TTCGGCTACC ATGTCGCCGG CAACGCGACG TTCCTCCGGT 
GCCCCTCTCT CAATGGTGCT GCCGCATGAA ACGGATGTAC ACTCCCCGGC GGCAGCCAAC 
GCCTTCTTTC ACGAGACCCG CACCCTGCCT CGCCAACAGC ATCGGCATCA TCCGAACAGT 
CCCGGCAGCA TGTCGGTACA ACATCAGCGC ACCGCCTCCG CAGCGGCTGC CATGATGAGC 
TTGACGGCAG CAGCTGCTCC CAAGCAGCAA GCGGCGGCGC AGACTGAGCA CAAGCTGCAG 
TACTTTGATC TGGATGTGAC CAACAAACCG CCATTGCTAA ATCGATCCAG CATGAGTGTG 
GGGAATCTGT ACTCACAAGG CGGAAATGGA GCTAGTGGAA TGCGATTCGC AGGCGTCGAA 
GCTGGCGGAG CTCGAGCGCC AGTGCCTAGC AGCGTGGTCT ATAGATCCGT GGACTTTGTC 
AAGACGGAGG CATTTAAGAG GATTCGAGAG GAGCGCGAGA GCAGCGGCAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523890.2
CDS	74..2710
Translation	MDRTFYEGWLIKSPPTKRIWRARWRRRYFTLKQGEIPEQFCLEYYTDHNCRKLKGVIDLDQCEQVDCGLRLENRKQKFQYMFDIKTPKRTYYLAAETEADMRDWVNCICQVCHLHDTKQSNELPLGAVGADENRTQHTSSSGGLSNSTQNTTTTSLHSSAGTTAPQASVPNAGGSAQLRRPAVIEEQPMPSNAGNNNSDSVYVNTEYSNRETMLCDANFDQQELLSAAQQQPPPSPATALYLNHSALIQAQAAAAAAEQLQQQQQQAARLAVSANGVVRKLPEHLVLTQQTLAEAAAQQHSSVQASPALSTASGPYIPISECFSGSPRFLPGVPLPGADLAIPNNPTTPLNNLDPKFYDTPRSHNNIGLNLTNDQSYSPKITNLSLQQLANNNASKQRSDSDSESVFTDDDEWAHPLPLRENVDRSTRPSDSSIENESFVLTYSQRFSKMPEEGGAIVPPAEKSSKLAGAASLPEAGDQGTLDKLAKVLKNKNNLILDFKENEKIPRDLPQLSDTENTSPAIVARRNAHSAFIEESYDIPRSHQQPYYNVNQLLGERPVTSPHNSNPIAASTPNLMAADLGAVAAISAAANPGLMGEAQAVASSPTSARTLPRHCYTNAAPTKMEGNVFRYDFMEQADCPPVNRKLKPKVAGGLPVVEDKPPEEFPAKPPVGVDQLTNKLGAAQLQQPIGPPSVDRKCKPNAYKLGNSATMSPATRRSSGAPLSMVLPHETDVHSPAAANAFFHETRTLPRQQHRHHPNSPGSMSVQHQRTASAAAAMMSLTAAAAPKQQAAAQTEHKLQYFDLDVTNKPPLLNRSSMSVGNLYSQGGNGASGMRFAGVEAGGARAPVPSSVVYRSVDFVKTEAFKRIREERESSGNK

Target ORF information:

RefSeq Version	NM_079166.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughter of sevenless (dos), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079166.3

ORF Insert Sequence:

ATGGATCGCA CTTTCTACGA GGGCTGGCTA ATTAAGTCGC CGCCCACCAA GCGCATTTGG 
CGAGCACGCT GGAGGCGTCG CTACTTCACG CTGAAGCAGG GCGAGATACC GGAGCAGTTC 
TGCCTCGAAT ACTACACCGA CCACAACTGC CGCAAGCTGA AGGGCGTTAT CGATTTGGAT 
CAGTGCGAGC AGGTGGACTG TGGCCTGCGG CTGGAGAATC GCAAGCAGAA GTTTCAGTAC 
ATGTTCGATA TCAAGACGCC CAAGCGCACC TACTACCTGG CTGCTGAGAC GGAGGCGGAT 
ATGCGCGACT GGGTGAACTG CATCTGCCAG GTGTGTCACC TACACGACAC AAAGCAATCG 
AATGAGTTGC CATTAGGAGC TGTGGGTGCA GACGAAAACC GCACCCAACA CACGAGCTCC 
AGTGGCGGTC TAAGCAACTC CACTCAAAAT ACGACCACCA CATCGTTGCA CTCGTCGGCC 
GGAACCACAG CGCCTCAGGC TTCGGTCCCA AATGCTGGAG GATCTGCCCA GCTGCGACGA 
CCGGCGGTCA TTGAAGAGCA ACCGATGCCC TCGAATGCCG GCAACAATAA CTCAGACTCG 
GTGTATGTCA ACACGGAGTA CAGCAATCGC GAGACGATGC TCTGCGATGC CAATTTCGAT 
CAGCAGGAAC TGCTCTCGGC TGCCCAGCAG CAACCGCCTC CATCGCCGGC CACGGCTCTT 
TATCTAAACC ACAGTGCTTT GATCCAAGCT CAGGCGGCAG CTGCAGCCGC CGAGCAACTG 
CAGCAGCAGC AGCAACAAGC TGCTCGCTTG GCGGTCAGTG CCAACGGAGT AGTGCGCAAA 
TTGCCGGAGC ATCTGGTGCT CACGCAGCAG ACCCTGGCGG AGGCAGCTGC CCAGCAACAC 
AGCAGCGTAC AGGCTTCACC CGCTTTGAGC ACCGCATCCG GACCGTACAT ACCCATCAGC 
GAGTGCTTCT CGGGCAGCCC GAGATTCCTG CCCGGCGTTC CACTGCCCGG CGCAGATTTG 
GCCATACCAA ACAACCCCAC CACGCCGTTG AACAACTTGG ATCCCAAGTT CTACGATACG 
CCGCGTAGTC ATAATAACAT AGGTCTAAAT CTGACCAACG ATCAATCCTA CTCACCGAAG 
ATAACCAATT TGAGTCTGCA ACAATTGGCC AATAACAATG CTAGTAAACA ACGCTCCGAC 
TCCGATTCGG AAAGCGTTTT CACCGATGAC GATGAGTGGG CTCATCCTTT GCCACTGCGC 
GAGAATGTGG ATCGCAGTAC CCGACCTTCG GATAGTTCCA TTGAAAACGA ATCATTCGTG 
CTAACGTACT CGCAGCGATT CTCTAAAATG CCCGAGGAAG GAGGAGCGAT CGTGCCACCA 
GCTGAAAAGA GTTCCAAGCT AGCAGGAGCA GCATCTCTGC CAGAGGCTGG TGACCAGGGA 
ACGCTGGATA AGCTGGCCAA GGTGCTGAAG AACAAAAATA ATCTCATATT GGACTTTAAG 
GAGAATGAAA AAATTCCTCG CGATCTGCCG CAGCTGTCGG ATACGGAAAA TACTTCGCCG 
GCCATTGTGG CCCGCCGCAA CGCGCACTCC GCTTTCATCG AGGAAAGCTA TGACATTCCA 
AGGTCCCACC AGCAGCCTTA CTACAACGTC AACCAGCTGC TGGGCGAGCG ACCAGTCACC 
AGCCCGCACA ATTCCAATCC CATTGCAGCA TCCACACCGA ATCTCATGGC GGCCGATCTG 
GGTGCAGTGG CCGCAATTTC AGCGGCAGCG AATCCCGGCC TGATGGGTGA GGCACAGGCA 
GTCGCATCAT CGCCCACCAG TGCGCGCACA TTGCCTCGAC ACTGCTACAC CAATGCAGCT 
CCCACAAAGA TGGAGGGCAA TGTTTTTCGA TACGACTTCA TGGAGCAGGC GGACTGCCCG 
CCGGTTAACC GGAAACTTAA GCCAAAGGTG GCGGGTGGAT TGCCAGTTGT AGAGGACAAA 
CCGCCGGAGG AGTTTCCTGC AAAGCCCCCG GTGGGCGTAG ATCAGTTAAC CAATAAACTG 
GGTGCAGCCC AACTGCAGCA GCCCATTGGA CCGCCCAGTG TGGACAGGAA GTGCAAACCG 
AATGCCTATA AGTTGGGCAA TTCGGCTACC ATGTCGCCGG CAACGCGACG TTCCTCCGGT 
GCCCCTCTCT CAATGGTGCT GCCGCATGAA ACGGATGTAC ACTCCCCGGC GGCAGCCAAC 
GCCTTCTTTC ACGAGACCCG CACCCTGCCT CGCCAACAGC ATCGGCATCA TCCGAACAGT 
CCCGGCAGCA TGTCGGTACA ACATCAGCGC ACCGCCTCCG CAGCGGCTGC CATGATGAGC 
TTGACGGCAG CAGCTGCTCC CAAGCAGCAA GCGGCGGCGC AGACTGAGCA CAAGCTGCAG 
TACTTTGATC TGGATGTGAC CAACAAACCG CCATTGCTAA ATCGATCCAG CATGAGTGTG 
GGGAATCTGT ACTCACAAGG CGGAAATGGA GCTAGTGGAA TGCGATTCGC AGGCGTCGAA 
GCTGGCGGAG CTCGAGCGCC AGTGCCTAGC AGCGTGGTCT ATAGATCCGT GGACTTTGTC 
AAGACGGAGG CATTTAAGAG GATTCGAGAG GAGCGCGAGA GCAGCGGCAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dos cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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