klar cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following klar gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klar cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa20359 NM_001103992.2
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa21965 NM_001299960.1
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa21966 NM_001299959.1
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OFa17075 NM_001274284.1
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa17074 NM_001274283.2
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa20360 NM_001103993.2
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa17073 NM_001274282.2
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa20357 NM_001043111.3
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa20358 NM_079149.5
Latest version!
Drosophila melanogaster klarsicht (klar), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa20359
    Clone ID Related Accession (Same CDS sequence) NM_001103992.2
    Accession Version NM_001103992.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001103992.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAAG TCAGCGTGGC CGTCCAGACG GATATATCCG AAATGCTTTC GGCCACTTCT 
    TCCACCAACA CATCACGCTC GTCATCGGAG CAAGATCTGC TCGGCGCCTG GGAGGATCTA
    CTTAGCTGGA GCGAGAATGC CTCCGCTGCC CGCAAATTGC AGCAGGAGAT GAGTGTGTTG
    AAGAGCTCGC TGCAGCGCCT GGGAGACAAG CCAACTCCAG AGCTCCTCGA TACGGAGCCG
    GCCATCCAAA TAGCAGTGGA GGCGCTCAAG TTGGAGCAGA CGCAACTGAC CAGCTATAGG
    ACCAACATGC TGCGTCTTAA CGCCTCCGTC CACAGTTGGC TTACCAAGCA GGAGCGGCGA
    CTGCAGAGCG CCTTGGAGGA ACAGGAGCAG CAACAGGAGT CCGAACAACT CAAGCAGCAA
    AAACTTGTTG AAGAGGAGAA GGGAGCTGAC GTTCAGAAGG AGCTGGCGTC TACCGGAGCA
    GTGGCCATCA CGGTCACCGA CAGCAATGGT AATCAGGTGG AGGCACTAGC CACAGGAGAA
    GCCTCCACCT CCACGCCAGC CTGGGATGTG CACACGCTGA TGTCATCGGA GCAGGAGTTC
    CATAAGCATC TGAAGAACGA AGTGAGCGAC ATGTACAGTT CCTGGGATGA GGCGGATGCC
    AGAATCAACA CGCAACTGGA AATGCTTACC AACTCGCTTA TTGCCTGGAG GCAGTTGGAG
    TCCGGTTTGA GTGAGTTCCA ATTGGCTTTG GGCCAAGATA GGGGCACTCT GAAGGGTCTT
    GAAGGAGCTC TGGACAAGGG ACAAGCCACA CCAGTCGAGC TGGCCCAGAA TGTAAAACTG
    GTGGCCAAGC TGCTGTCCGA AAAGGTTCAC GTAAGCCAGG AGCAACTTTT GGCCGTCCAG
    CAGCACCTGG ACCCCAACCA CATCTACCAC ATTACGAAGT TTACAGCCTC AAATGGCTCG
    CTATCAGATT CCGGCATCTC TGATGGAGGA GCTACTTCTG ATGGTGGCCT GTCCGAGAGG
    GAGCGACGAC TGGGAGTTCT TCGACGCCTA GCAAAGCAGT TGGAACTAGC TCTGGCGCCG
    GGAAGCGAAG CCATGCGTTC TATTGCTGCC CGAATGGAAA GTGCCGAGGC CGACCTGAAG
    CACCTGCAGA ACACCTGTAG AGACTTAATT GTTCGCACTG CAGCTAGCCA CCAGCAGAAG
    CAGCAGATCC AGCAAAATCA GACCCAGCAG GTGTCACCGA AGGCCAATGG ACACATTAAG
    AAGCAGGCCG CAAAGGGCAA GGCAGAACCC CAGTCGCCTG GCAGACGTGG TAAAGGAGCT
    CGAAAGGCGC GTCAGGCCAA GAAGGCTGGA GAGGATCAGC AAGTAGAGGA GCCAAGTCTC
    AGCCCAGAAC AGCAGAAGAT GGTCCTGAAG CAACTCAAGA CCTTGACAAG TGGCGATGGT
    GGGGACGACC CCTCTGACGA TCCCTCGTTG CTCTTCAACC TGGAAAGCTC CGAGGAAGAT
    GGAGAGGGAG CGGATCCTGC ACAGACCTCT AAGCGAGGGT GGGCGTGGCG CATCGCGAGG
    GCGGCAGTGC CTATGCAGGT GGCCCTGTTC ACAATCTTCT GCGCTGCCTG CCTGATGCAA
    CCCAACTGCT GCGACAATCT GAACAACCTG TCCATGAGCT TCACGCCGCA GCTCCGCTAT
    ATCCGTGGAC CACCTCCGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097462.1
    CDS510..2213
    Translation

    Target ORF information:

    RefSeq Version NM_001103992.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAAG TCAGCGTGGC CGTCCAGACG GATATATCCG AAATGCTTTC GGCCACTTCT 
    TCCACCAACA CATCACGCTC GTCATCGGAG CAAGATCTGC TCGGCGCCTG GGAGGATCTA
    CTTAGCTGGA GCGAGAATGC CTCCGCTGCC CGCAAATTGC AGCAGGAGAT GAGTGTGTTG
    AAGAGCTCGC TGCAGCGCCT GGGAGACAAG CCAACTCCAG AGCTCCTCGA TACGGAGCCG
    GCCATCCAAA TAGCAGTGGA GGCGCTCAAG TTGGAGCAGA CGCAACTGAC CAGCTATAGG
    ACCAACATGC TGCGTCTTAA CGCCTCCGTC CACAGTTGGC TTACCAAGCA GGAGCGGCGA
    CTGCAGAGCG CCTTGGAGGA ACAGGAGCAG CAACAGGAGT CCGAACAACT CAAGCAGCAA
    AAACTTGTTG AAGAGGAGAA GGGAGCTGAC GTTCAGAAGG AGCTGGCGTC TACCGGAGCA
    GTGGCCATCA CGGTCACCGA CAGCAATGGT AATCAGGTGG AGGCACTAGC CACAGGAGAA
    GCCTCCACCT CCACGCCAGC CTGGGATGTG CACACGCTGA TGTCATCGGA GCAGGAGTTC
    CATAAGCATC TGAAGAACGA AGTGAGCGAC ATGTACAGTT CCTGGGATGA GGCGGATGCC
    AGAATCAACA CGCAACTGGA AATGCTTACC AACTCGCTTA TTGCCTGGAG GCAGTTGGAG
    TCCGGTTTGA GTGAGTTCCA ATTGGCTTTG GGCCAAGATA GGGGCACTCT GAAGGGTCTT
    GAAGGAGCTC TGGACAAGGG ACAAGCCACA CCAGTCGAGC TGGCCCAGAA TGTAAAACTG
    GTGGCCAAGC TGCTGTCCGA AAAGGTTCAC GTAAGCCAGG AGCAACTTTT GGCCGTCCAG
    CAGCACCTGG ACCCCAACCA CATCTACCAC ATTACGAAGT TTACAGCCTC AAATGGCTCG
    CTATCAGATT CCGGCATCTC TGATGGAGGA GCTACTTCTG ATGGTGGCCT GTCCGAGAGG
    GAGCGACGAC TGGGAGTTCT TCGACGCCTA GCAAAGCAGT TGGAACTAGC TCTGGCGCCG
    GGAAGCGAAG CCATGCGTTC TATTGCTGCC CGAATGGAAA GTGCCGAGGC CGACCTGAAG
    CACCTGCAGA ACACCTGTAG AGACTTAATT GTTCGCACTG CAGCTAGCCA CCAGCAGAAG
    CAGCAGATCC AGCAAAATCA GACCCAGCAG GTGTCACCGA AGGCCAATGG ACACATTAAG
    AAGCAGGCCG CAAAGGGCAA GGCAGAACCC CAGTCGCCTG GCAGACGTGG TAAAGGAGCT
    CGAAAGGCGC GTCAGGCCAA GAAGGCTGGA GAGGATCAGC AAGTAGAGGA GCCAAGTCTC
    AGCCCAGAAC AGCAGAAGAT GGTCCTGAAG CAACTCAAGA CCTTGACAAG TGGCGATGGT
    GGGGACGACC CCTCTGACGA TCCCTCGTTG CTCTTCAACC TGGAAAGCTC CGAGGAAGAT
    GGAGAGGGAG CGGATCCTGC ACAGACCTCT AAGCGAGGGT GGGCGTGGCG CATCGCGAGG
    GCGGCAGTGC CTATGCAGGT GGCCCTGTTC ACAATCTTCT GCGCTGCCTG CCTGATGCAA
    CCCAACTGCT GCGACAATCT GAACAACCTG TCCATGAGCT TCACGCCGCA GCTCCGCTAT
    ATCCGTGGAC CACCTCCGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21965
    Clone ID Related Accession (Same CDS sequence) NM_001299960.1
    Accession Version NM_001299960.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTGTGT TGAAGAGCTC GCTGCAGCGC CTGGGAGACA AGCCAACTCC AGAGCTCCTC 
    GATACGGAGC CGGCCATCCA AATAGCAGTG GAGGCGCTCA AGTTGGAGCA GACGCAACTG
    ACCAGCTATA GGACCAACAT GCTGCGTCTT AACGCCTCCG TCCACAGTTG GCTTACCAAG
    CAGGAGCGGC GACTGCAGAG CGCCTTGGAG GAACAGGAGC AGCAACAGGA GTCCGAACAA
    CTCAAGCAGC AAAAACTTGT TGAAGAGGAG AAGGGAGCTG ACGTTCAGAA GGAGCTGGCG
    TCTACCGGAG CAGTGGCCAT CACGGTCACC GACAGCAATG GTAATCAGGT GGAGGCACTA
    GCCACAGGAG AAGCCTCCAC CTCCACGCCA GCCTGGGATG TGCACACGCT GATGTCATCG
    GAGCAGGAGT TCCATAAGCA TCTGAAGAAC GAAGTGAGCG ACATGTACAG TTCCTGGGAT
    GAGGCGGATG CCAGAATCAA CACGCAACTG GAAATGCTTA CCAACTCGCT TATTGCCTGG
    AGGCAGTTGG AGTCCGGTTT GAGTGAGTTC CAATTGGCTT TGGGCCAAGA TAGGGGCACT
    CTGAAGGGTC TTGAAGGAGC TCTGGACAAG GGACAAGCCA CACCAGTCGA GCTGGCCCAG
    AATGTAAAAC TGGTGGCCAA GCTGCTGTCC GAAAAGGTTC ACGTAAGCCA GGAGCAACTT
    TTGGCCGTCC AGCAGCACCT GGACCCCAAC CACATCTACC ACATTACGAA GTTTACAGCC
    TCAAATGGCT CGCTATCAGA TTCCGGCATC TCTGATGGAG GAGCTACTTC TGATGGTGGC
    CTGTCCGAGA GGGAGCGACG ACTGGGAGTT CTTCGACGCC TAGCAAAGCA GTTGGAACTA
    GCTCTGGCGC CGGGAAGCGA AGCCATGCGT TCTATTGCTG CCCGAATGGA AAGTGCCGAG
    GCCGACCTGA AGCACCTGCA GAACACCTGT AGAGACTTAA TTGTTCGCAC TGCAGCTAGC
    CACCAGCAGA AGCAGCAGAT CCAGCAAAAT CAGACCCAGC AGGTGTCACC GAAGGCCAAT
    GGACACATTA AGAAGCAGGC CGCAAAGGGC AAGGCAGAAC CCCAGTCGCC TGGCAGACGT
    GGTAAAGGAG CTCGAAAGGC GCGTCAGGCC AAGAAGGCTG GAGAGGATCA GCAAGTAGAG
    GAGCCAAGTC TCAGCCCAGA ACAGCAGAAG ATGGTCCTGA AGCAACTCAA GACCTTGACA
    AGTGGCGATG GTGGGGACGA CCCCTCTGAC GATCCCTCGT TGCTCTTCAA CCTGGAAAGC
    TCCGAGGAAG ATGGAGAGGG AGCGGATCCT GCACAGACCT CTAAGCGAGG GTGGGCGTGG
    CGCATCGCGA GGGCGGCAGT GCCTATGCAG GTGGCCCTGT TCACAATCTT CTGCGCTGCC
    TGCCTGATGC AACCCAACTG CTGCGACAAT CTGAACAACC TGTCCATGAG CTTCACGCCG
    CAGCTCCGCT ATATCCGTGG ACCACCTCCG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286889.1
    CDS354..1889
    Translation

    Target ORF information:

    RefSeq Version NM_001299960.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTGTGT TGAAGAGCTC GCTGCAGCGC CTGGGAGACA AGCCAACTCC AGAGCTCCTC 
    GATACGGAGC CGGCCATCCA AATAGCAGTG GAGGCGCTCA AGTTGGAGCA GACGCAACTG
    ACCAGCTATA GGACCAACAT GCTGCGTCTT AACGCCTCCG TCCACAGTTG GCTTACCAAG
    CAGGAGCGGC GACTGCAGAG CGCCTTGGAG GAACAGGAGC AGCAACAGGA GTCCGAACAA
    CTCAAGCAGC AAAAACTTGT TGAAGAGGAG AAGGGAGCTG ACGTTCAGAA GGAGCTGGCG
    TCTACCGGAG CAGTGGCCAT CACGGTCACC GACAGCAATG GTAATCAGGT GGAGGCACTA
    GCCACAGGAG AAGCCTCCAC CTCCACGCCA GCCTGGGATG TGCACACGCT GATGTCATCG
    GAGCAGGAGT TCCATAAGCA TCTGAAGAAC GAAGTGAGCG ACATGTACAG TTCCTGGGAT
    GAGGCGGATG CCAGAATCAA CACGCAACTG GAAATGCTTA CCAACTCGCT TATTGCCTGG
    AGGCAGTTGG AGTCCGGTTT GAGTGAGTTC CAATTGGCTT TGGGCCAAGA TAGGGGCACT
    CTGAAGGGTC TTGAAGGAGC TCTGGACAAG GGACAAGCCA CACCAGTCGA GCTGGCCCAG
    AATGTAAAAC TGGTGGCCAA GCTGCTGTCC GAAAAGGTTC ACGTAAGCCA GGAGCAACTT
    TTGGCCGTCC AGCAGCACCT GGACCCCAAC CACATCTACC ACATTACGAA GTTTACAGCC
    TCAAATGGCT CGCTATCAGA TTCCGGCATC TCTGATGGAG GAGCTACTTC TGATGGTGGC
    CTGTCCGAGA GGGAGCGACG ACTGGGAGTT CTTCGACGCC TAGCAAAGCA GTTGGAACTA
    GCTCTGGCGC CGGGAAGCGA AGCCATGCGT TCTATTGCTG CCCGAATGGA AAGTGCCGAG
    GCCGACCTGA AGCACCTGCA GAACACCTGT AGAGACTTAA TTGTTCGCAC TGCAGCTAGC
    CACCAGCAGA AGCAGCAGAT CCAGCAAAAT CAGACCCAGC AGGTGTCACC GAAGGCCAAT
    GGACACATTA AGAAGCAGGC CGCAAAGGGC AAGGCAGAAC CCCAGTCGCC TGGCAGACGT
    GGTAAAGGAG CTCGAAAGGC GCGTCAGGCC AAGAAGGCTG GAGAGGATCA GCAAGTAGAG
    GAGCCAAGTC TCAGCCCAGA ACAGCAGAAG ATGGTCCTGA AGCAACTCAA GACCTTGACA
    AGTGGCGATG GTGGGGACGA CCCCTCTGAC GATCCCTCGT TGCTCTTCAA CCTGGAAAGC
    TCCGAGGAAG ATGGAGAGGG AGCGGATCCT GCACAGACCT CTAAGCGAGG GTGGGCGTGG
    CGCATCGCGA GGGCGGCAGT GCCTATGCAG GTGGCCCTGT TCACAATCTT CTGCGCTGCC
    TGCCTGATGC AACCCAACTG CTGCGACAAT CTGAACAACC TGTCCATGAG CTTCACGCCG
    CAGCTCCGCT ATATCCGTGG ACCACCTCCG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21966
    Clone ID Related Accession (Same CDS sequence) NM_001299959.1
    Accession Version NM_001299959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCCAGA GCAGCAACAG CAATATCAGC AGCAGCAACT CGTTGCTACA GCAACGAAAT 
    CACAATATCG GCAACAAAAT GCTGCCCAAC AAAGCGCACG GCAAGAACAT TGCCGTGGCG
    GTAATCACAC CCAATTCACA TGGAAACACC AGTCACGGTC ATGGGCTCGG TCACGGACTC
    GGCCACGAGC TGAACAAGTC GCCTTTGGGG CTGCGCAAGA CGCGTCATCA CCACAATGAC
    ACCTCCAAGT TCAACCGATC CAATCGCAAG TCGAAGAACT GTGCGATCTT CTATTTCAAG
    CACCTGGACA CGGACAACGA GCAGGGAAAC GCGGCGGGCA GTGATCTCCA GAGCGAGGAT
    GATCCGAGCC TGATCCATCG GCGCAGAGCT GGAGGCGACA TACTGAAATC GGCTGATGCC
    TCAACGGATG ACGACGACGA AGGTTGGCTC TACACGGCGA CAGCAGCAGC AACATTGGAA
    GTTGCAACAG CAGCAACTGC AGCACCGACT GCCGCAGCAG CAACATCGAG TGTGGATGGT
    CTGCAGTCCA CGGCCGTCTC CTCGACTACG GCCACGGGTG GACCACTCCC GCCCAGCGAC
    GACTCTGATA AGGAGAACAA GGTGGCTCTG GTCAGCGCCA CGACAATCAC GGCGGCAGCA
    ACCGCAACTG CAACATCCAT TGCAACACTG CACAGCAGCA ACTACGACAG CAGCAGCGCC
    TGTTCCTCCT CCAATTCAAA TTCGAATTCG AACTCCAACT CGAACGGCAG GCTCACGGAG
    ACCTCAGCCA CCTCGCGAGT AACCCAGCTA CAGATGCAAA TCCACTCGCA ATCGCAGTCG
    CAGTCACAGA TGGAGCTCCA AATCAATGGC AATGCCATCG ACGGCAGACA CATCATTAGC
    AACAACAATT GCTACAGCAG CATGCAACAT CAGCCGCAAA ACAACAACGA GGGTGAGGCA
    GCCGAGGATC TGGCCAAGAT CAAGATGGGT GACGATGAAG CAGCTGCTGA TATGGCAAAT
    GGAAACGCCA CCAAATCTCA GCAGATGAGC AACGGTGTGT ACAGTCGCGC GGACTCTTGC
    AACTTCACCG TTTGGGCGGC AGAAACTGTA GCCAGTTGCC ACTTGCCCCC GAGATCGCCG
    GCCAAATCGG CCAAGTCCTC GAAATCCCAG GCCAGCAATG CCACCGTGTC TGGATCCACG
    CTTGTTTCCC CAGTCAAGGG CAAGGTCTCG CATGATTCAA TCAAGCAGTT GGTGCTGGAA
    GCCGAGCATT TGGTGCGCGA CGCCCAAGAG ACCGCACTGA AGACGCCAAC CAAGCAGAAA
    CATTCCATCA TTAAGATCTC GTCGACGGTC AAGAAGCGCG AGGTCACTAT GCCCCATCCC
    ATCAAGCAGC GTGTGGAGGA ATGGCTGGAG CACCAGCCCT CGACCCCACA GCTGCTGACC
    CGGAGCCACA CGCACGAGCT CCTGCCCAGC TGCAAGCCAG ATGACTGTGA GGCCTCCGGA
    GAAGCATCCG AAACAGACTC GGTGCCCCAA GCTGGAGCAG GAGTAAACGG AGGAGCACCG
    AATGGTGCGG GATCGGATAC ATCGGAGGGC TTCACCGATT CCATTGCCAC ATGTATGCAA
    ACGAGCACGA ATTCCTATGG GAATTCCACG GAGAGAATCG GTGGATCGGC TGAACCCATT
    GGACAGCCCG TCACGCCTTT GGGCTTTGGC AGCAGCAACC AGAGCCTGAA TGTAAAGATT
    GTAAAGCGAT CGCAAACGCG TCGCAAGTCG GAGAGACCAT GGTCGGTCTC GTGTCTGTCC
    CAGTTGACTA CGGATGCAGC CCAGTTAACC ACGGCCCGGA TAGTGGAAAA CTCTCCGTCC
    GGTCTGGCCA GTCACTCGAT CAGTGAGAGT GCCCTGGACT CGCTTTCGCC AGGACCAAGA
    CCGCGGGCAG CTTCTTCCTC GGGAACGGGA AGCAATGCAG CCAAGAAGGC GGATAGCAAG
    GGCTCATTGA GGAGGAGGAA GGCCAGGAAG AAGCGCATTT CGGCCGCATC GGCTGGCAGG
    AAATCTGATT CAGGTTCCGA ACTGGGTGGA GATCTCACCC AGACACTGAT GAAGTCCTGC
    GAGTCCATGT CCTCGCAGCA GCTGCAGGAG TTCACCAATG CCCTTCTGAG CATTCAAAAA
    GGCGCAGTAG TTGCTCCTTT GAGTCCCAAG GGCGACGTTT CTGGAGTACC TTCACTGGCC
    GATGGCGAGG GAGGGGAAAC CCAGCTGATG CTACCCAAGT TCCGGGTGGG CTCCTTCACC
    ACAGCTGGCC TATTGGCTAC TGATACGAGG TTAGGCGCTT TAGCAGCCCT CTCAAACTAC
    ATGAACGAGG ACGAACAACA AGCAGAACTC AGCACTGAGG ACCATCATAG CAGCATCTCG
    GAGACGGCGT GGGACAACTA CCAAGAGAAA TACAACTCGG AGAACTATTC CGAGGGCTTT
    GATTCAGATG CGGCCCGGCG TCTCTTGGAA TTCGGCGACG ACTATCGCAA TTTCATCGAC
    TCGCAATCGG ACTGCTGCAG TTCTCTGTCG GCGGCCAACA ATCTGGACTC GTTCTCGCCG
    CCGCGCATGG ACTCACTGCA GAAGCACGAG CTGAAGACCC TCCATATCAA CCAGGACACA
    ATCACCAGCA CCGTGGACCA CGCCCGCCGC CAGCGCGCCC TTGAACTGCA GTACGAGCGT
    CGTCGCAAGA CCCTGGAAGT TCGACGAAAG TCTTGCCAAG ACATGGATGA GAGCCTGATG
    GCTAGTCCCC AGTCGGATCA ACAGCAGCAG CAATTGCAGG TCACGCCTTC GTTGTCTGCC
    TCCGCCACCG CCCTAATGAC CACGCCCAAG ATTCAGTCGA CCAGCCACCA GATTAGCCAT
    CGCGCAGAAT CTGTGGGACG CAAGTTGGAT TTCGGCGGAA TGAGCCACTC CGCTCAGTCC
    CTGCTGCGTC GCACCTCGGA GAGCGATACT TCAACCAGGA GACGACGCAC TGTGACGGCG
    GACGAAAGAA GACGCTCCTC CCGCAACCTG GAGAAGTGCA TCAAACTTAT TCCAGCTACC
    ACTTCCTCGT CGAGTGGCTC GGACTCCGAG GACGGAGAGC AGGAGATGCG ATCCCTGCTG
    CAGCAGAGCC GCGACAGGCT GGACGACACC AGAGCCCTGA AGATCAGATG CCATCTGCTT
    AGACCAGAGG ATTATAACGA GATCATCAAC ACTTGCCGCG ACAACATTCG CTGCCTGGAG
    GCCGTGCTGC GCGGTCCGCC CGGCACCGTG TTGTCCAACC ACTGCGCTGG CCAGACAAAA
    GTATCAGCCT GTCTCCGCCG GCGCCGGAGG CGACGTCTAG GGACTGAGCT TCCCGGTCCC
    ACGGAATCCG GCGATTCCCG GCAGATGGTG CATATGGTCG AGTGCCAGTG TGCGCCCATT
    ACGCGCTTCG TGGCACGGGT GATCGAGTTC GTGCTGGACG TGGGCAATAT GCTGGAGCAC
    TTTGCGCTCT ACCGATTCCT GGCGGGAACG CTGAGGTCGC TCTACGAGCT GGTAGACCGG
    CCCGTGGGTG GACGCCTCGG TCGGTTCTTT GACGGGCTGC GGGGACTGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286888.1
    CDS1799..5392
    Translation

    Target ORF information:

    RefSeq Version NM_001299959.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCCAGA GCAGCAACAG CAATATCAGC AGCAGCAACT CGTTGCTACA GCAACGAAAT 
    CACAATATCG GCAACAAAAT GCTGCCCAAC AAAGCGCACG GCAAGAACAT TGCCGTGGCG
    GTAATCACAC CCAATTCACA TGGAAACACC AGTCACGGTC ATGGGCTCGG TCACGGACTC
    GGCCACGAGC TGAACAAGTC GCCTTTGGGG CTGCGCAAGA CGCGTCATCA CCACAATGAC
    ACCTCCAAGT TCAACCGATC CAATCGCAAG TCGAAGAACT GTGCGATCTT CTATTTCAAG
    CACCTGGACA CGGACAACGA GCAGGGAAAC GCGGCGGGCA GTGATCTCCA GAGCGAGGAT
    GATCCGAGCC TGATCCATCG GCGCAGAGCT GGAGGCGACA TACTGAAATC GGCTGATGCC
    TCAACGGATG ACGACGACGA AGGTTGGCTC TACACGGCGA CAGCAGCAGC AACATTGGAA
    GTTGCAACAG CAGCAACTGC AGCACCGACT GCCGCAGCAG CAACATCGAG TGTGGATGGT
    CTGCAGTCCA CGGCCGTCTC CTCGACTACG GCCACGGGTG GACCACTCCC GCCCAGCGAC
    GACTCTGATA AGGAGAACAA GGTGGCTCTG GTCAGCGCCA CGACAATCAC GGCGGCAGCA
    ACCGCAACTG CAACATCCAT TGCAACACTG CACAGCAGCA ACTACGACAG CAGCAGCGCC
    TGTTCCTCCT CCAATTCAAA TTCGAATTCG AACTCCAACT CGAACGGCAG GCTCACGGAG
    ACCTCAGCCA CCTCGCGAGT AACCCAGCTA CAGATGCAAA TCCACTCGCA ATCGCAGTCG
    CAGTCACAGA TGGAGCTCCA AATCAATGGC AATGCCATCG ACGGCAGACA CATCATTAGC
    AACAACAATT GCTACAGCAG CATGCAACAT CAGCCGCAAA ACAACAACGA GGGTGAGGCA
    GCCGAGGATC TGGCCAAGAT CAAGATGGGT GACGATGAAG CAGCTGCTGA TATGGCAAAT
    GGAAACGCCA CCAAATCTCA GCAGATGAGC AACGGTGTGT ACAGTCGCGC GGACTCTTGC
    AACTTCACCG TTTGGGCGGC AGAAACTGTA GCCAGTTGCC ACTTGCCCCC GAGATCGCCG
    GCCAAATCGG CCAAGTCCTC GAAATCCCAG GCCAGCAATG CCACCGTGTC TGGATCCACG
    CTTGTTTCCC CAGTCAAGGG CAAGGTCTCG CATGATTCAA TCAAGCAGTT GGTGCTGGAA
    GCCGAGCATT TGGTGCGCGA CGCCCAAGAG ACCGCACTGA AGACGCCAAC CAAGCAGAAA
    CATTCCATCA TTAAGATCTC GTCGACGGTC AAGAAGCGCG AGGTCACTAT GCCCCATCCC
    ATCAAGCAGC GTGTGGAGGA ATGGCTGGAG CACCAGCCCT CGACCCCACA GCTGCTGACC
    CGGAGCCACA CGCACGAGCT CCTGCCCAGC TGCAAGCCAG ATGACTGTGA GGCCTCCGGA
    GAAGCATCCG AAACAGACTC GGTGCCCCAA GCTGGAGCAG GAGTAAACGG AGGAGCACCG
    AATGGTGCGG GATCGGATAC ATCGGAGGGC TTCACCGATT CCATTGCCAC ATGTATGCAA
    ACGAGCACGA ATTCCTATGG GAATTCCACG GAGAGAATCG GTGGATCGGC TGAACCCATT
    GGACAGCCCG TCACGCCTTT GGGCTTTGGC AGCAGCAACC AGAGCCTGAA TGTAAAGATT
    GTAAAGCGAT CGCAAACGCG TCGCAAGTCG GAGAGACCAT GGTCGGTCTC GTGTCTGTCC
    CAGTTGACTA CGGATGCAGC CCAGTTAACC ACGGCCCGGA TAGTGGAAAA CTCTCCGTCC
    GGTCTGGCCA GTCACTCGAT CAGTGAGAGT GCCCTGGACT CGCTTTCGCC AGGACCAAGA
    CCGCGGGCAG CTTCTTCCTC GGGAACGGGA AGCAATGCAG CCAAGAAGGC GGATAGCAAG
    GGCTCATTGA GGAGGAGGAA GGCCAGGAAG AAGCGCATTT CGGCCGCATC GGCTGGCAGG
    AAATCTGATT CAGGTTCCGA ACTGGGTGGA GATCTCACCC AGACACTGAT GAAGTCCTGC
    GAGTCCATGT CCTCGCAGCA GCTGCAGGAG TTCACCAATG CCCTTCTGAG CATTCAAAAA
    GGCGCAGTAG TTGCTCCTTT GAGTCCCAAG GGCGACGTTT CTGGAGTACC TTCACTGGCC
    GATGGCGAGG GAGGGGAAAC CCAGCTGATG CTACCCAAGT TCCGGGTGGG CTCCTTCACC
    ACAGCTGGCC TATTGGCTAC TGATACGAGG TTAGGCGCTT TAGCAGCCCT CTCAAACTAC
    ATGAACGAGG ACGAACAACA AGCAGAACTC AGCACTGAGG ACCATCATAG CAGCATCTCG
    GAGACGGCGT GGGACAACTA CCAAGAGAAA TACAACTCGG AGAACTATTC CGAGGGCTTT
    GATTCAGATG CGGCCCGGCG TCTCTTGGAA TTCGGCGACG ACTATCGCAA TTTCATCGAC
    TCGCAATCGG ACTGCTGCAG TTCTCTGTCG GCGGCCAACA ATCTGGACTC GTTCTCGCCG
    CCGCGCATGG ACTCACTGCA GAAGCACGAG CTGAAGACCC TCCATATCAA CCAGGACACA
    ATCACCAGCA CCGTGGACCA CGCCCGCCGC CAGCGCGCCC TTGAACTGCA GTACGAGCGT
    CGTCGCAAGA CCCTGGAAGT TCGACGAAAG TCTTGCCAAG ACATGGATGA GAGCCTGATG
    GCTAGTCCCC AGTCGGATCA ACAGCAGCAG CAATTGCAGG TCACGCCTTC GTTGTCTGCC
    TCCGCCACCG CCCTAATGAC CACGCCCAAG ATTCAGTCGA CCAGCCACCA GATTAGCCAT
    CGCGCAGAAT CTGTGGGACG CAAGTTGGAT TTCGGCGGAA TGAGCCACTC CGCTCAGTCC
    CTGCTGCGTC GCACCTCGGA GAGCGATACT TCAACCAGGA GACGACGCAC TGTGACGGCG
    GACGAAAGAA GACGCTCCTC CCGCAACCTG GAGAAGTGCA TCAAACTTAT TCCAGCTACC
    ACTTCCTCGT CGAGTGGCTC GGACTCCGAG GACGGAGAGC AGGAGATGCG ATCCCTGCTG
    CAGCAGAGCC GCGACAGGCT GGACGACACC AGAGCCCTGA AGATCAGATG CCATCTGCTT
    AGACCAGAGG ATTATAACGA GATCATCAAC ACTTGCCGCG ACAACATTCG CTGCCTGGAG
    GCCGTGCTGC GCGGTCCGCC CGGCACCGTG TTGTCCAACC ACTGCGCTGG CCAGACAAAA
    GTATCAGCCT GTCTCCGCCG GCGCCGGAGG CGACGTCTAG GGACTGAGCT TCCCGGTCCC
    ACGGAATCCG GCGATTCCCG GCAGATGGTG CATATGGTCG AGTGCCAGTG TGCGCCCATT
    ACGCGCTTCG TGGCACGGGT GATCGAGTTC GTGCTGGACG TGGGCAATAT GCTGGAGCAC
    TTTGCGCTCT ACCGATTCCT GGCGGGAACG CTGAGGTCGC TCTACGAGCT GGTAGACCGG
    CCCGTGGGTG GACGCCTCGG TCGGTTCTTT GACGGGCTGC GGGGACTGTT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17075
    Clone ID Related Accession (Same CDS sequence) NM_001274284.1
    Accession Version NM_001274284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCCATC CCATCAAGCA GCGTGTGGAG GAATGGCTGG AGCACCAGCC CTCGACCCCA 
    CAGCTGCTGA CCCGGAGCCA CACGCACGAG CTCCTGCCCA GCTGCAAGCC AGATGACTGT
    GAGGCCTCCG GAGAAGCATC CGAAACAGAC TCGGTGCCCC AAGCTGGAGC AGGAGTAAAC
    GGAGGAGCAC CGAATGGTGC GGGATCGGAT ACATCGGAGG GCTTCACCGA TTCCATTGCC
    ACATGTATGC AAACGAGCAC GAATTCCTAT GGGAATTCCA CGGAGAGAAT CGGTGGATCG
    GCTGAACCCA TTGGACAGCC CGTCACGCCT TTGGGCTTTG GCAGCAGCAA CCAGAGCCTG
    AATGTAAAGA TTGTAAAGCG ATCGCAAACG CGTCGCAAGT CGGAGAGACC ATGGTCGGTC
    TCGTGTCTGT CCCAGTTGAC TACGGATGCA GCCCAGTTAA CCACGGCCCG GATAGTGGAA
    AACTCTCCGT CCGGTCTGGC CAGTCACTCG ATCAGTGAGA GTGCCCTGGA CTCGCTTTCG
    CCAGGACCAA GACCGCGGGC AGCTTCTTCC TCGGGAACGG GAAGCAATGC AGCCAAGAAG
    GCGGATAGCA AGGGCTCATT GAGGAGGAGG AAGGCCAGGA AGAAGCGCAT TTCGGCCGCA
    TCGGCTGGCA GGAAATCTGA TTCAGGTTCC GAACTGGGTG GAGATCTCAC CCAGACACTG
    ATGAAGTCCT GCGAGTCCAT GTCCTCGCAG CAGCTGCAGG AGTTCACCAA TGCCCTTCTG
    AGCATTCAAA AAGGCGCAGT AGTTGCTCCT TTGAGTCCCA AGGGCGACGT TTCTGGAGTA
    CCTTCACTGG CCGATGGCGA GGGAGGGGAA ACCCAGCTGA TGCTACCCAA GTTCCGGGTG
    GGCTCCTTCA CCACAGCTGG CCTATTGGCT ACTGATACGA GGTTAGGCGC TTTAGCAGCC
    CTCTCAAACT ACATGAACGA GGACGAACAA CAAGCAGAAC TCAGCACTGA GGACCATCAT
    AGCAGCATCT CGGAGACGGC GTGGGACAAC TACCAAGAGA AATACAACTC GGAGAACTAT
    TCCGAGGGCT TTGATTCAGA TGCGGCCCGG CGTCTCTTGG AATTCGGCGA CGACTATCGC
    AATTTCATCG ACTCGCAATC GGACTGCTGC AGTTCTCTGT CGGCGGCCAA CAATCTGGAC
    TCGTTCTCGC CGCCGCGCAT GGACTCACTG CAGAAGCACG AGCTGAAGAC CCTCCATATC
    AACCAGGACA CAATCACCAG CACCGTGGAC CACGCCCGCC GCCAGCGCGC CCTTGAACTG
    CAGTACGAGC GTCGTCGCAA GACCCTGGAA GTTCGACGAA AGTCTTGCCA AGACATGGAT
    GAGAGCCTGA TGGCTAGTCC CCAGTCGGAT CAACAGCAGC AGCAATTGCA GGTCACGCCT
    TCGTTGTCTG CCTCCGCCAC CGCCCTAATG ACCACGCCCA AGATTCAGTC GACCAGCCAC
    CAGATTAGCC ATCGCGCAGA ATCTGTGGGA CGCAAGTTGG ATTTCGGCGG AATGAGCCAC
    TCCGCTCAGT CCCTGCTGCG TCGCACCTCG GAGAGCGATA CTTCAACCAG GAGACGACGC
    ACTGTGACGG CGGACGAAAG AAGACGCTCC TCCCGCAACC TGGAGAAGTG CATCAAACTT
    ATTCCAGCTA CCACTTCCTC GTCGAGTGGC TCGGACTCCG AGGACGGAGA GCAGGAGATG
    CGATCCCTGC TGCAGCAGAG CCGCGACAGG CTGGACGACA CCAGAGCCCT GAAGATCAGA
    TGCCATCTGC TTAGACCAGA GGATTATAAC GAGATCATCA ACACTTGCCG CGACAACATT
    CGCTGCCTGG AGGCCGTGCT GCGCGGTCCG CCCGGCACCG TGTTGTCCAA CCACTGCGCT
    GGCCAGACAA AAGATCTGCT CGGCGCCTGG GAGGATCTAC TTAGCTGGAG CGAGAATGCC
    TCCGCTGCCC GCAAATTGCA GCAGGAGATG AGTGTGTTGA AGAGCTCGCT GCAGCGCCTG
    GGAGACAAGC CAACTCCAGA GCTCCTCGAT ACGGAGCCGG CCATCCAAAT AGCAGTGGAG
    GCGCTCAAGT TGGAGCAGAC GCAACTGACC AGCTATAGGA CCAACATGCT GCGTCTTAAC
    GCCTCCGTCC ACAGTTGGCT TACCAAGCAG GAGCGGCGAC TGCAGAGCGC CTTGGAGGAA
    CAGGAGCAGC AACAGGAGTC CGAACAACTC AAGCAGCAAA AACTTGTTGA AGAGGAGAAG
    GGAGCTGACG TTCAGAAGGA GCTGGCGTCT ACCGGAGCAG TGGCCATCAC GGTCACCGAC
    AGCAATGGTA ATCAGGTGGA GGCACTAGCC ACAGGAGAAG CCTCCACCTC CACGCCAGCC
    TGGGATGTGC ACACGCTGAT GTCATCGGAG CAGGAGTTCC ATAAGCATCT GAAGAACGAA
    GTGAGCGACA TGTACAGTTC CTGGGATGAG GCGGATGCCA GAATCAACAC GCAACTGGAA
    ATGCTTACCA ACTCGCTTAT TGCCTGGAGG CAGTTGGAGT CCGGTTTGAG TGAGTTCCAA
    TTGGCTTTGG GCCAAGATAG GGGCACTCTG AAGGGTCTTG AAGGAGCTCT GGACAAGGGA
    CAAGCCACAC CAGTCGAGCT GGCCCAGAAT GTAAAACTGG TGGCCAAGCT GCTGTCCGAA
    AAGGTTCACG TAAGCCAGGA GCAACTTTTG GCCGTCCAGC AGCACCTGGA CCCCAACCAC
    ATCTACCACA TTACGAAGTT TACAGCCTCA AATGGCTCGC TATCAGATTC CGGCATCTCT
    GATGGAGGAG CTACTTCTGA TGGTGGCCTG TCCGAGAGGG AGCGACGACT GGGAGTTCTT
    CGACGCCTAG CAAAGCAGTT GGAACTAGCT CTGGCGCCGG GAAGCGAAGC CATGCGTTCT
    ATTGCTGCCC GAATGGAAAG TGCCGAGGCC GACCTGAAGC ACCTGCAGAA CACCTGTAGA
    GACTTAATTG TTCGCACTGC AGCTAGCCAC CAGCAGAAGC AGCAGATCCA GCAAAATCAG
    ACCCAGCAGG TGTCACCGAA GGCCAATGGA CACATTAAGA AGCAGGCCGC AAAGGGCAAG
    GCAGAACCCC AGTCGCCTGG CAGACGTGGT AAAGGAGCTC GAAAGGCGCG TCAGGCCAAG
    AAGGCTGGAG AGGATCAGCA AGTAGAGGAG CCAAGTCTCA GCCCAGAACA GCAGAAGATG
    GTCCTGAAGC AACTCAAGAC CTTGACAAGT GGCGATGGTG GGGACGACCC CTCTGACGAT
    CCCTCGTTGC TCTTCAACCT GGAAAGCTCC GAGGAAGATG GAGAGGGAGC GGATCCTGCA
    CAGACCTCTA AGCGAGGGTG GGCGTGGCGC ATCGCGAGGG CGGCAGTGCC TATGCAGGTG
    GCCCTGTTCA CAATCTTCTG CGCTGCCTGC CTGATGCAAC CCAACTGCTG CGACAATCTG
    AACAACCTGT CCATGAGCTT CACGCCGCAG CTCCGCTATA TCCGTGGACC ACCTCCGATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261213.1
    CDS573..4115
    Translation

    Target ORF information:

    RefSeq Version NM_001274284.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCCATC CCATCAAGCA GCGTGTGGAG GAATGGCTGG AGCACCAGCC CTCGACCCCA 
    CAGCTGCTGA CCCGGAGCCA CACGCACGAG CTCCTGCCCA GCTGCAAGCC AGATGACTGT
    GAGGCCTCCG GAGAAGCATC CGAAACAGAC TCGGTGCCCC AAGCTGGAGC AGGAGTAAAC
    GGAGGAGCAC CGAATGGTGC GGGATCGGAT ACATCGGAGG GCTTCACCGA TTCCATTGCC
    ACATGTATGC AAACGAGCAC GAATTCCTAT GGGAATTCCA CGGAGAGAAT CGGTGGATCG
    GCTGAACCCA TTGGACAGCC CGTCACGCCT TTGGGCTTTG GCAGCAGCAA CCAGAGCCTG
    AATGTAAAGA TTGTAAAGCG ATCGCAAACG CGTCGCAAGT CGGAGAGACC ATGGTCGGTC
    TCGTGTCTGT CCCAGTTGAC TACGGATGCA GCCCAGTTAA CCACGGCCCG GATAGTGGAA
    AACTCTCCGT CCGGTCTGGC CAGTCACTCG ATCAGTGAGA GTGCCCTGGA CTCGCTTTCG
    CCAGGACCAA GACCGCGGGC AGCTTCTTCC TCGGGAACGG GAAGCAATGC AGCCAAGAAG
    GCGGATAGCA AGGGCTCATT GAGGAGGAGG AAGGCCAGGA AGAAGCGCAT TTCGGCCGCA
    TCGGCTGGCA GGAAATCTGA TTCAGGTTCC GAACTGGGTG GAGATCTCAC CCAGACACTG
    ATGAAGTCCT GCGAGTCCAT GTCCTCGCAG CAGCTGCAGG AGTTCACCAA TGCCCTTCTG
    AGCATTCAAA AAGGCGCAGT AGTTGCTCCT TTGAGTCCCA AGGGCGACGT TTCTGGAGTA
    CCTTCACTGG CCGATGGCGA GGGAGGGGAA ACCCAGCTGA TGCTACCCAA GTTCCGGGTG
    GGCTCCTTCA CCACAGCTGG CCTATTGGCT ACTGATACGA GGTTAGGCGC TTTAGCAGCC
    CTCTCAAACT ACATGAACGA GGACGAACAA CAAGCAGAAC TCAGCACTGA GGACCATCAT
    AGCAGCATCT CGGAGACGGC GTGGGACAAC TACCAAGAGA AATACAACTC GGAGAACTAT
    TCCGAGGGCT TTGATTCAGA TGCGGCCCGG CGTCTCTTGG AATTCGGCGA CGACTATCGC
    AATTTCATCG ACTCGCAATC GGACTGCTGC AGTTCTCTGT CGGCGGCCAA CAATCTGGAC
    TCGTTCTCGC CGCCGCGCAT GGACTCACTG CAGAAGCACG AGCTGAAGAC CCTCCATATC
    AACCAGGACA CAATCACCAG CACCGTGGAC CACGCCCGCC GCCAGCGCGC CCTTGAACTG
    CAGTACGAGC GTCGTCGCAA GACCCTGGAA GTTCGACGAA AGTCTTGCCA AGACATGGAT
    GAGAGCCTGA TGGCTAGTCC CCAGTCGGAT CAACAGCAGC AGCAATTGCA GGTCACGCCT
    TCGTTGTCTG CCTCCGCCAC CGCCCTAATG ACCACGCCCA AGATTCAGTC GACCAGCCAC
    CAGATTAGCC ATCGCGCAGA ATCTGTGGGA CGCAAGTTGG ATTTCGGCGG AATGAGCCAC
    TCCGCTCAGT CCCTGCTGCG TCGCACCTCG GAGAGCGATA CTTCAACCAG GAGACGACGC
    ACTGTGACGG CGGACGAAAG AAGACGCTCC TCCCGCAACC TGGAGAAGTG CATCAAACTT
    ATTCCAGCTA CCACTTCCTC GTCGAGTGGC TCGGACTCCG AGGACGGAGA GCAGGAGATG
    CGATCCCTGC TGCAGCAGAG CCGCGACAGG CTGGACGACA CCAGAGCCCT GAAGATCAGA
    TGCCATCTGC TTAGACCAGA GGATTATAAC GAGATCATCA ACACTTGCCG CGACAACATT
    CGCTGCCTGG AGGCCGTGCT GCGCGGTCCG CCCGGCACCG TGTTGTCCAA CCACTGCGCT
    GGCCAGACAA AAGATCTGCT CGGCGCCTGG GAGGATCTAC TTAGCTGGAG CGAGAATGCC
    TCCGCTGCCC GCAAATTGCA GCAGGAGATG AGTGTGTTGA AGAGCTCGCT GCAGCGCCTG
    GGAGACAAGC CAACTCCAGA GCTCCTCGAT ACGGAGCCGG CCATCCAAAT AGCAGTGGAG
    GCGCTCAAGT TGGAGCAGAC GCAACTGACC AGCTATAGGA CCAACATGCT GCGTCTTAAC
    GCCTCCGTCC ACAGTTGGCT TACCAAGCAG GAGCGGCGAC TGCAGAGCGC CTTGGAGGAA
    CAGGAGCAGC AACAGGAGTC CGAACAACTC AAGCAGCAAA AACTTGTTGA AGAGGAGAAG
    GGAGCTGACG TTCAGAAGGA GCTGGCGTCT ACCGGAGCAG TGGCCATCAC GGTCACCGAC
    AGCAATGGTA ATCAGGTGGA GGCACTAGCC ACAGGAGAAG CCTCCACCTC CACGCCAGCC
    TGGGATGTGC ACACGCTGAT GTCATCGGAG CAGGAGTTCC ATAAGCATCT GAAGAACGAA
    GTGAGCGACA TGTACAGTTC CTGGGATGAG GCGGATGCCA GAATCAACAC GCAACTGGAA
    ATGCTTACCA ACTCGCTTAT TGCCTGGAGG CAGTTGGAGT CCGGTTTGAG TGAGTTCCAA
    TTGGCTTTGG GCCAAGATAG GGGCACTCTG AAGGGTCTTG AAGGAGCTCT GGACAAGGGA
    CAAGCCACAC CAGTCGAGCT GGCCCAGAAT GTAAAACTGG TGGCCAAGCT GCTGTCCGAA
    AAGGTTCACG TAAGCCAGGA GCAACTTTTG GCCGTCCAGC AGCACCTGGA CCCCAACCAC
    ATCTACCACA TTACGAAGTT TACAGCCTCA AATGGCTCGC TATCAGATTC CGGCATCTCT
    GATGGAGGAG CTACTTCTGA TGGTGGCCTG TCCGAGAGGG AGCGACGACT GGGAGTTCTT
    CGACGCCTAG CAAAGCAGTT GGAACTAGCT CTGGCGCCGG GAAGCGAAGC CATGCGTTCT
    ATTGCTGCCC GAATGGAAAG TGCCGAGGCC GACCTGAAGC ACCTGCAGAA CACCTGTAGA
    GACTTAATTG TTCGCACTGC AGCTAGCCAC CAGCAGAAGC AGCAGATCCA GCAAAATCAG
    ACCCAGCAGG TGTCACCGAA GGCCAATGGA CACATTAAGA AGCAGGCCGC AAAGGGCAAG
    GCAGAACCCC AGTCGCCTGG CAGACGTGGT AAAGGAGCTC GAAAGGCGCG TCAGGCCAAG
    AAGGCTGGAG AGGATCAGCA AGTAGAGGAG CCAAGTCTCA GCCCAGAACA GCAGAAGATG
    GTCCTGAAGC AACTCAAGAC CTTGACAAGT GGCGATGGTG GGGACGACCC CTCTGACGAT
    CCCTCGTTGC TCTTCAACCT GGAAAGCTCC GAGGAAGATG GAGAGGGAGC GGATCCTGCA
    CAGACCTCTA AGCGAGGGTG GGCGTGGCGC ATCGCGAGGG CGGCAGTGCC TATGCAGGTG
    GCCCTGTTCA CAATCTTCTG CGCTGCCTGC CTGATGCAAC CCAACTGCTG CGACAATCTG
    AACAACCTGT CCATGAGCTT CACGCCGCAG CTCCGCTATA TCCGTGGACC ACCTCCGATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17074
    Clone ID Related Accession (Same CDS sequence) NM_001274283.2
    Accession Version NM_001274283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274283.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCCCAGA GCAGCAACAG CAATATCAGC AGCAGCAACT CGTTGCTACA GCAACGAAAT 
    CACAATATCG GCAACAAAAT GCTGCCCAAC AAAGCGCACG GCAAGAACAT TGCCGTGGCG
    GTAATCACAC CCAATTCACA TGGAAACACC AGTCACGGTC ATGGGCTCGG TCACGGACTC
    GGCCACGAGC TGAACAAGTC GCCTTTGGGG CTGCGCAAGA CGCGTCATCA CCACAATGAC
    ACCTCCAAGT TCAACCGATC CAATCGCAAG TCGAAGAACT GTGCGATCTT CTATTTCAAG
    CACCTGGACA CGGACAACGA GCAGGGAAAC GCGGCGGGCA GTGATCTCCA GAGCGAGGAT
    GATCCGAGCC TGATCCATCG GCGCAGAGCT GGAGGCGACA TACTGAAATC GGCTGATGCC
    TCAACGGATG ACGACGACGA AGGTTGGCTC TACACGGCGA CAGCAGCAGC AACATTGGAA
    GTTGCAACAG CAGCAACTGC AGCACCGACT GCCGCAGCAG CAACATCGAG TGTGGATGGT
    CTGCAGTCCA CGGCCGTCTC CTCGACTACG GCCACGGGTG GACCACTCCC GCCCAGCGAC
    GACTCTGATA AGGAGAACAA GGTGGCTCTG GTCAGCGCCA CGACAATCAC GGCGGCAGCA
    ACCGCAACTG CAACATCCAT TGCAACACTG CACAGCAGCA ACTACGACAG CAGCAGCGCC
    TGTTCCTCCT CCAATTCAAA TTCGAATTCG AACTCCAACT CGAACGGCAG GCTCACGGAG
    ACCTCAGCCA CCTCGCGAGT AACCCAGCTA CAGATGCAAA TCCACTCGCA ATCGCAGTCG
    CAGTCACAGA TGGAGCTCCA AATCAATGGC AATGCCATCG ACGGCAGACA CATCATTAGC
    AACAACAATT GCTACAGCAG CATGCAACAT CAGCCGCAAA ACAACAACGA GGGTGAGGCA
    GCCGAGGATC TGGCCAAGAT CAAGATGGGT GACGATGAAG CAGCTGCTGA TATGGCAAAT
    GGAAACGCCA CCAAATCTCA GCAGATGAGC AACGGTGTGT ACAGTCGCGC GGACTCTTGC
    AACTTCACCG TTTGGGCGGC AGAAACTGTA GCCAGTTGCC ACTTGCCCCC GAGATCGCCG
    GCCAAATCGG CCAAGTCCTC GAAATCCCAG GCCAGCAATG CCACCGTGTC TGGATCCACG
    CTTGTTTCCC CAGTCAAGGG CAAGGTCTCG CATGATTCAA TCAAGCAGTT GGTGCTGGAA
    GCCGAGCATT TGGTGCGCGA CGCCCAAGAG ACCGCACTGA AGACGCCAAC CAAGCAGAAA
    CATTCCATCA TTAAGATCTC GTCGACGGTC AAGAAGCGCG AGGTCACTAT GCCCCATCCC
    ATCAAGCAGC GTGTGGAGGA ATGGCTGGAG CACCAGCCCT CGACCCCACA GCTGCTGACC
    CGGAGCCACA CGCACGAGCT CCTGCCCAGC TGCAAGCCAG ATGACTGTGA GGCCTCCGGA
    GAAGCATCCG AAACAGACTC GGTGCCCCAA GCTGGAGCAG GAGTAAACGG AGGAGCACCG
    AATGGTGCGG GATCGGATAC ATCGGAGGGC TTCACCGATT CCATTGCCAC ATGTATGCAA
    ACGAGCACGA ATTCCTATGG GAATTCCACG GAGAGAATCG GTGGATCGGC TGAACCCATT
    GGACAGCCCG TCACGCCTTT GGGCTTTGGC AGCAGCAACC AGAGCCTGAA TGTAAAGATT
    GTAAAGCGAT CGCAAACGCG TCGCAAGTCG GAGAGACCAT GGTCGGTCTC GTGTCTGTCC
    CAGTTGACTA CGGATGCAGC CCAGTTAACC ACGGCCCGGA TAGTGGAAAA CTCTCCGTCC
    GGTCTGGCCA GTCACTCGAT CAGTGAGAGT GCCCTGGACT CGCTTTCGCC AGGACCAAGA
    CCGCGGGCAG CTTCTTCCTC GGGAACGGGA AGCAATGCAG CCAAGAAGGC GGATAGCAAG
    GGCTCATTGA GGAGGAGGAA GGCCAGGAAG AAGCGCATTT CGGCCGCATC GGCTGGCAGG
    AAATCTGATT CAGGTTCCGA ACTGGGTGGA GATCTCACCC AGACACTGAT GAAGTCCTGC
    GAGTCCATGT CCTCGCAGCA GCTGCAGGAG TTCACCAATG CCCTTCTGAG CATTCAAAAA
    GGCGCAGTAG TTGCTCCTTT GAGTCCCAAG GGCGACGTTT CTGGAGTACC TTCACTGGCC
    GATGGCGAGG GAGGGGAAAC CCAGCTGATG CTACCCAAGT TCCGGGTGGG CTCCTTCACC
    ACAGCTGGCC TATTGGCTAC TGATACGAGG TTAGGCGCTT TAGCAGCCCT CTCAAACTAC
    ATGAACGAGG ACGAACAACA AGCAGAACTC AGCACTGAGG ACCATCATAG CAGCATCTCG
    GAGACGGCGT GGGACAACTA CCAAGAGAAA TACAACTCGG AGAACTATTC CGAGGGCTTT
    GATTCAGATG CGGCCCGGCG TCTCTTGGAA TTCGGCGACG ACTATCGCAA TTTCATCGAC
    TCGCAATCGG ACTGCTGCAG TTCTCTGTCG GCGGCCAACA ATCTGGACTC GTTCTCGCCG
    CCGCGCATGG ACTCACTGCA GAAGCACGAG CTGAAGACCC TCCATATCAA CCAGGACACA
    ATCACCAGCA CCGTGGACCA CGCCCGCCGC CAGCGCGCCC TTGAACTGCA GTACGAGCGT
    CGTCGCAAGA CCCTGGAAGT TCGACGAAAG TCTTGCCAAG ACATGGATGA GAGCCTGATG
    GCTAGTCCCC AGTCGGATCA ACAGCAGCAG CAATTGCAGG TCACGCCTTC GTTGTCTGCC
    TCCGCCACCG CCCTAATGAC CACGCCCAAG ATTCAGTCGA CCAGCCACCA GATTAGCCAT
    CGCGCAGAAT CTGTGGGACG CAAGTTGGAT TTCGGCGGAA TGAGCCACTC CGCTCAGTCC
    CTGCTGCGTC GCACCTCGGA GAGCGATACT TCAACCAGGA GACGACGCAC TGTGACGGCG
    GACGAAAGAA GACGCTCCTC CCGCAACCTG GAGAAGTGCA TCAAACTTAT TCCAGCTACC
    ACTTCCTCGT CGAGTGGCTC GGACTCCGAG GACGGAGAGC AGGAGATGCG ATCCCTGCTG
    CAGCAGAGCC GCGACAGGCT GGACGACACC AGAGCCCTGA AGATCAGATG CCATCTGCTT
    AGACCAGAGG ATTATAACGA GATCATCAAC ACTTGCCGCG ACAACATTCG CTGCCTGGAG
    GCCGTGCTGC GCGGTCCGCC CGGCACCGTG TTGTCCAACC ACTGCGCTGG CCAGACAAAA
    GATCTGCTCG GCGCCTGGGA GGATCTACTT AGCTGGAGCG AGAATGCCTC CGCTGCCCGC
    AAATTGCAGC AGGAGATGAG TGTGTTGAAG AGCTCGCTGC AGCGCCTGGG AGACAAGCCA
    ACTCCAGAGC TCCTCGATAC GGAGCCGGCC ATCCAAATAG CAGTGGAGGC GCTCAAGTTG
    GAGCAGACGC AACTGACCAG CTATAGGACC AACATGCTGC GTCTTAACGC CTCCGTCCAC
    AGTTGGCTTA CCAAGCAGGA GCGGCGACTG CAGAGCGCCT TGGAGGAACA GGAGCAGCAA
    CAGGAGTCCG AACAACTCAA GCAGCAAAAA CTTGTTGAAG AGGAGAAGGG AGCTGACGTT
    CAGAAGGAGC TGGCGTCTAC CGGAGCAGTG GCCATCACGG TCACCGACAG CAATGGTAAT
    CAGGTGGAGG CACTAGCCAC AGGAGAAGCC TCCACCTCCA CGCCAGCCTG GGATGTGCAC
    ACGCTGATGT CATCGGAGCA GGAGTTCCAT AAGCATCTGA AGAACGAAGT GAGCGACATG
    TACAGTTCCT GGGATGAGGC GGATGCCAGA ATCAACACGC AACTGGAAAT GCTTACCAAC
    TCGCTTATTG CCTGGAGGCA GTTGGAGTCC GGTTTGAGTG AGTTCCAATT GGCTTTGGGC
    CAAGATAGGG GCACTCTGAA GGGTCTTGAA GGAGCTCTGG ACAAGGGACA AGCCACACCA
    GTCGAGCTGG CCCAGAATGT AAAACTGGTG GCCAAGCTGC TGTCCGAAAA GGTTCACGTA
    AGCCAGGAGC AACTTTTGGC CGTCCAGCAG CACCTGGACC CCAACCACAT CTACCACATT
    ACGAAGTTTA CAGCCTCAAA TGGCTCGCTA TCAGATTCCG GCATCTCTGA TGGAGGAGCT
    ACTTCTGATG GTGGCCTGTC CGAGAGGGAG CGACGACTGG GAGTTCTTCG ACGCCTAGCA
    AAGCAGTTGG AACTAGCTCT GGCGCCGGGA AGCGAAGCCA TGCGTTCTAT TGCTGCCCGA
    ATGGAAAGTG CCGAGGCCGA CCTGAAGCAC CTGCAGAACA CCTGTAGAGA CTTAATTGTT
    CGCACTGCAG CTAGCCACCA GCAGAAGCAG CAGATCCAGC AAAATCAGAC CCAGCAGGTG
    TCACCGAAGG CCAATGGACA CATTAAGAAG CAGGCCGCAA AGGGCAAGGC AGAACCCCAG
    TCGCCTGGCA GACGTGGTAA AGGAGCTCGA AAGGCGCGTC AGGCCAAGAA GGCTGGAGAG
    GATCAGCAAG TAGAGGAGCC AAGTCTCAGC CCAGAACAGC AGAAGATGGT CCTGAAGCAA
    CTCAAGACCT TGACAAGTGG CGATGGTGGG GACGACCCCT CTGACGATCC CTCGTTGCTC
    TTCAACCTGG AAAGCTCCGA GGAAGATGGA GAGGGAGCGG ATCCTGCACA GACCTCTAAG
    CGAGGGTGGG CGTGGCGCAT CGCGAGGGCG GCAGTGCCTA TGCAGGTGGC CCTGTTCACA
    ATCTTCTGCG CTGCCTGCCT GATGCAACCC AACTGCTGCG ACAATCTGAA CAACCTGTCC
    ATGAGCTTCA CGCCGCAGCT CCGCTATATC CGTGGACCAC CTCCGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261212.1
    CDS1799..6709
    Translation

    Target ORF information:

    RefSeq Version NM_001274283.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCCCAGA GCAGCAACAG CAATATCAGC AGCAGCAACT CGTTGCTACA GCAACGAAAT 
    CACAATATCG GCAACAAAAT GCTGCCCAAC AAAGCGCACG GCAAGAACAT TGCCGTGGCG
    GTAATCACAC CCAATTCACA TGGAAACACC AGTCACGGTC ATGGGCTCGG TCACGGACTC
    GGCCACGAGC TGAACAAGTC GCCTTTGGGG CTGCGCAAGA CGCGTCATCA CCACAATGAC
    ACCTCCAAGT TCAACCGATC CAATCGCAAG TCGAAGAACT GTGCGATCTT CTATTTCAAG
    CACCTGGACA CGGACAACGA GCAGGGAAAC GCGGCGGGCA GTGATCTCCA GAGCGAGGAT
    GATCCGAGCC TGATCCATCG GCGCAGAGCT GGAGGCGACA TACTGAAATC GGCTGATGCC
    TCAACGGATG ACGACGACGA AGGTTGGCTC TACACGGCGA CAGCAGCAGC AACATTGGAA
    GTTGCAACAG CAGCAACTGC AGCACCGACT GCCGCAGCAG CAACATCGAG TGTGGATGGT
    CTGCAGTCCA CGGCCGTCTC CTCGACTACG GCCACGGGTG GACCACTCCC GCCCAGCGAC
    GACTCTGATA AGGAGAACAA GGTGGCTCTG GTCAGCGCCA CGACAATCAC GGCGGCAGCA
    ACCGCAACTG CAACATCCAT TGCAACACTG CACAGCAGCA ACTACGACAG CAGCAGCGCC
    TGTTCCTCCT CCAATTCAAA TTCGAATTCG AACTCCAACT CGAACGGCAG GCTCACGGAG
    ACCTCAGCCA CCTCGCGAGT AACCCAGCTA CAGATGCAAA TCCACTCGCA ATCGCAGTCG
    CAGTCACAGA TGGAGCTCCA AATCAATGGC AATGCCATCG ACGGCAGACA CATCATTAGC
    AACAACAATT GCTACAGCAG CATGCAACAT CAGCCGCAAA ACAACAACGA GGGTGAGGCA
    GCCGAGGATC TGGCCAAGAT CAAGATGGGT GACGATGAAG CAGCTGCTGA TATGGCAAAT
    GGAAACGCCA CCAAATCTCA GCAGATGAGC AACGGTGTGT ACAGTCGCGC GGACTCTTGC
    AACTTCACCG TTTGGGCGGC AGAAACTGTA GCCAGTTGCC ACTTGCCCCC GAGATCGCCG
    GCCAAATCGG CCAAGTCCTC GAAATCCCAG GCCAGCAATG CCACCGTGTC TGGATCCACG
    CTTGTTTCCC CAGTCAAGGG CAAGGTCTCG CATGATTCAA TCAAGCAGTT GGTGCTGGAA
    GCCGAGCATT TGGTGCGCGA CGCCCAAGAG ACCGCACTGA AGACGCCAAC CAAGCAGAAA
    CATTCCATCA TTAAGATCTC GTCGACGGTC AAGAAGCGCG AGGTCACTAT GCCCCATCCC
    ATCAAGCAGC GTGTGGAGGA ATGGCTGGAG CACCAGCCCT CGACCCCACA GCTGCTGACC
    CGGAGCCACA CGCACGAGCT CCTGCCCAGC TGCAAGCCAG ATGACTGTGA GGCCTCCGGA
    GAAGCATCCG AAACAGACTC GGTGCCCCAA GCTGGAGCAG GAGTAAACGG AGGAGCACCG
    AATGGTGCGG GATCGGATAC ATCGGAGGGC TTCACCGATT CCATTGCCAC ATGTATGCAA
    ACGAGCACGA ATTCCTATGG GAATTCCACG GAGAGAATCG GTGGATCGGC TGAACCCATT
    GGACAGCCCG TCACGCCTTT GGGCTTTGGC AGCAGCAACC AGAGCCTGAA TGTAAAGATT
    GTAAAGCGAT CGCAAACGCG TCGCAAGTCG GAGAGACCAT GGTCGGTCTC GTGTCTGTCC
    CAGTTGACTA CGGATGCAGC CCAGTTAACC ACGGCCCGGA TAGTGGAAAA CTCTCCGTCC
    GGTCTGGCCA GTCACTCGAT CAGTGAGAGT GCCCTGGACT CGCTTTCGCC AGGACCAAGA
    CCGCGGGCAG CTTCTTCCTC GGGAACGGGA AGCAATGCAG CCAAGAAGGC GGATAGCAAG
    GGCTCATTGA GGAGGAGGAA GGCCAGGAAG AAGCGCATTT CGGCCGCATC GGCTGGCAGG
    AAATCTGATT CAGGTTCCGA ACTGGGTGGA GATCTCACCC AGACACTGAT GAAGTCCTGC
    GAGTCCATGT CCTCGCAGCA GCTGCAGGAG TTCACCAATG CCCTTCTGAG CATTCAAAAA
    GGCGCAGTAG TTGCTCCTTT GAGTCCCAAG GGCGACGTTT CTGGAGTACC TTCACTGGCC
    GATGGCGAGG GAGGGGAAAC CCAGCTGATG CTACCCAAGT TCCGGGTGGG CTCCTTCACC
    ACAGCTGGCC TATTGGCTAC TGATACGAGG TTAGGCGCTT TAGCAGCCCT CTCAAACTAC
    ATGAACGAGG ACGAACAACA AGCAGAACTC AGCACTGAGG ACCATCATAG CAGCATCTCG
    GAGACGGCGT GGGACAACTA CCAAGAGAAA TACAACTCGG AGAACTATTC CGAGGGCTTT
    GATTCAGATG CGGCCCGGCG TCTCTTGGAA TTCGGCGACG ACTATCGCAA TTTCATCGAC
    TCGCAATCGG ACTGCTGCAG TTCTCTGTCG GCGGCCAACA ATCTGGACTC GTTCTCGCCG
    CCGCGCATGG ACTCACTGCA GAAGCACGAG CTGAAGACCC TCCATATCAA CCAGGACACA
    ATCACCAGCA CCGTGGACCA CGCCCGCCGC CAGCGCGCCC TTGAACTGCA GTACGAGCGT
    CGTCGCAAGA CCCTGGAAGT TCGACGAAAG TCTTGCCAAG ACATGGATGA GAGCCTGATG
    GCTAGTCCCC AGTCGGATCA ACAGCAGCAG CAATTGCAGG TCACGCCTTC GTTGTCTGCC
    TCCGCCACCG CCCTAATGAC CACGCCCAAG ATTCAGTCGA CCAGCCACCA GATTAGCCAT
    CGCGCAGAAT CTGTGGGACG CAAGTTGGAT TTCGGCGGAA TGAGCCACTC CGCTCAGTCC
    CTGCTGCGTC GCACCTCGGA GAGCGATACT TCAACCAGGA GACGACGCAC TGTGACGGCG
    GACGAAAGAA GACGCTCCTC CCGCAACCTG GAGAAGTGCA TCAAACTTAT TCCAGCTACC
    ACTTCCTCGT CGAGTGGCTC GGACTCCGAG GACGGAGAGC AGGAGATGCG ATCCCTGCTG
    CAGCAGAGCC GCGACAGGCT GGACGACACC AGAGCCCTGA AGATCAGATG CCATCTGCTT
    AGACCAGAGG ATTATAACGA GATCATCAAC ACTTGCCGCG ACAACATTCG CTGCCTGGAG
    GCCGTGCTGC GCGGTCCGCC CGGCACCGTG TTGTCCAACC ACTGCGCTGG CCAGACAAAA
    GATCTGCTCG GCGCCTGGGA GGATCTACTT AGCTGGAGCG AGAATGCCTC CGCTGCCCGC
    AAATTGCAGC AGGAGATGAG TGTGTTGAAG AGCTCGCTGC AGCGCCTGGG AGACAAGCCA
    ACTCCAGAGC TCCTCGATAC GGAGCCGGCC ATCCAAATAG CAGTGGAGGC GCTCAAGTTG
    GAGCAGACGC AACTGACCAG CTATAGGACC AACATGCTGC GTCTTAACGC CTCCGTCCAC
    AGTTGGCTTA CCAAGCAGGA GCGGCGACTG CAGAGCGCCT TGGAGGAACA GGAGCAGCAA
    CAGGAGTCCG AACAACTCAA GCAGCAAAAA CTTGTTGAAG AGGAGAAGGG AGCTGACGTT
    CAGAAGGAGC TGGCGTCTAC CGGAGCAGTG GCCATCACGG TCACCGACAG CAATGGTAAT
    CAGGTGGAGG CACTAGCCAC AGGAGAAGCC TCCACCTCCA CGCCAGCCTG GGATGTGCAC
    ACGCTGATGT CATCGGAGCA GGAGTTCCAT AAGCATCTGA AGAACGAAGT GAGCGACATG
    TACAGTTCCT GGGATGAGGC GGATGCCAGA ATCAACACGC AACTGGAAAT GCTTACCAAC
    TCGCTTATTG CCTGGAGGCA GTTGGAGTCC GGTTTGAGTG AGTTCCAATT GGCTTTGGGC
    CAAGATAGGG GCACTCTGAA GGGTCTTGAA GGAGCTCTGG ACAAGGGACA AGCCACACCA
    GTCGAGCTGG CCCAGAATGT AAAACTGGTG GCCAAGCTGC TGTCCGAAAA GGTTCACGTA
    AGCCAGGAGC AACTTTTGGC CGTCCAGCAG CACCTGGACC CCAACCACAT CTACCACATT
    ACGAAGTTTA CAGCCTCAAA TGGCTCGCTA TCAGATTCCG GCATCTCTGA TGGAGGAGCT
    ACTTCTGATG GTGGCCTGTC CGAGAGGGAG CGACGACTGG GAGTTCTTCG ACGCCTAGCA
    AAGCAGTTGG AACTAGCTCT GGCGCCGGGA AGCGAAGCCA TGCGTTCTAT TGCTGCCCGA
    ATGGAAAGTG CCGAGGCCGA CCTGAAGCAC CTGCAGAACA CCTGTAGAGA CTTAATTGTT
    CGCACTGCAG CTAGCCACCA GCAGAAGCAG CAGATCCAGC AAAATCAGAC CCAGCAGGTG
    TCACCGAAGG CCAATGGACA CATTAAGAAG CAGGCCGCAA AGGGCAAGGC AGAACCCCAG
    TCGCCTGGCA GACGTGGTAA AGGAGCTCGA AAGGCGCGTC AGGCCAAGAA GGCTGGAGAG
    GATCAGCAAG TAGAGGAGCC AAGTCTCAGC CCAGAACAGC AGAAGATGGT CCTGAAGCAA
    CTCAAGACCT TGACAAGTGG CGATGGTGGG GACGACCCCT CTGACGATCC CTCGTTGCTC
    TTCAACCTGG AAAGCTCCGA GGAAGATGGA GAGGGAGCGG ATCCTGCACA GACCTCTAAG
    CGAGGGTGGG CGTGGCGCAT CGCGAGGGCG GCAGTGCCTA TGCAGGTGGC CCTGTTCACA
    ATCTTCTGCG CTGCCTGCCT GATGCAACCC AACTGCTGCG ACAATCTGAA CAACCTGTCC
    ATGAGCTTCA CGCCGCAGCT CCGCTATATC CGTGGACCAC CTCCGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20360
    Clone ID Related Accession (Same CDS sequence) NM_001103993.2
    Accession Version NM_001103993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001103993.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGAACTCAG CACTGAGGAC
    CATCATAGCA GCATCTCGGA GACGGCGTGG GACAACTACC AAGAGAAATA CAACTCGGAG
    AACTATTCCG AGGGCTTTGA TTCAGATGCG GCCCGGCGTC TCTTGGAATT CGGCGACGAC
    TATCGCAATT TCATCGACTC GCAATCGGAC TGCTGCAGTT CTCTGTCGGC GGCCAACAAT
    CTGGACTCGT TCTCGCCGCC GCGCATGGAC TCACTGCAGA AGCACGAGCT GAAGACCCTC
    CATATCAACC AGGACACAAT CACCAGCACC GTGGACCACG CCCGCCGCCA GCGCGCCCTT
    GAACTGCAGT ACGAGCGTCG TCGCAAGACC CTGGAAGTTC GACGAAAGTC TTGCCAAGAC
    ATGGATGAGA GCCTGATGGC TAGTCCCCAG TCGGATCAAC AGCAGCAGCA ATTGCAGGTC
    ACGCCTTCGT TGTCTGCCTC CGCCACCGCC CTAATGACCA CGCCCAAGAT TCAGTCGACC
    AGCCACCAGA TTAGCCATCG CGCAGAATCT GTGGGACGCA AGTTGGATTT CGGCGGAATG
    AGCCACTCCG CTCAGTCCCT GCTGCGTCGC ACCTCGGAGA GCGATACTTC AACCAGGAGA
    CGACGCACTG TGACGGCGGA CGAAAGAAGA CGCTCCTCCC GCAACCTGGA GAAGTGCATC
    AAACTTATTC CAGCTACCAC TTCCTCGTCG AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG
    GAGATGCGAT CCCTGCTGCA GCAGAGCCGC GACAGGCTGG ACGACACCAG AGCCCTGAAG
    ATCAGATGCC ATCTGCTTAG ACCAGAGGAT TATAACGAGA TCATCAACAC TTGCCGCGAC
    AACATTCGCT GCCTGGAGGC CGTGCTGCGC GGTCCGCCCG GCACCGTGTT GTCCAACCAC
    TGCGCTGGCC AGACAAAAGT ATCAGCCTGT CTCCGCCGGC GCCGGAGGCG ACGTCTAGGG
    ACTGAGCTTC CCGGTCCCAC GGAATCCGGC GATTCCCGGC AGATGGTGCA TATGGTCGAG
    TGCCAGTGTG CGCCCATTAC GCGCTTCGTG GCACGGGTGA TCGAGTTCGT GCTGGACGTG
    GGCAATATGC TGGAGCACTT TGCGCTCTAC CGATTCCTGG CGGGAACGCT GAGGTCGCTC
    TACGAGCTGG TAGACCGGCC CGTGGGTGGA CGCCTCGGTC GGTTCTTTGA CGGGCTGCGG
    GGACTGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097463.1
    CDS1099..6570
    Translation

    Target ORF information:

    RefSeq Version NM_001103993.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGAACTCAG CACTGAGGAC
    CATCATAGCA GCATCTCGGA GACGGCGTGG GACAACTACC AAGAGAAATA CAACTCGGAG
    AACTATTCCG AGGGCTTTGA TTCAGATGCG GCCCGGCGTC TCTTGGAATT CGGCGACGAC
    TATCGCAATT TCATCGACTC GCAATCGGAC TGCTGCAGTT CTCTGTCGGC GGCCAACAAT
    CTGGACTCGT TCTCGCCGCC GCGCATGGAC TCACTGCAGA AGCACGAGCT GAAGACCCTC
    CATATCAACC AGGACACAAT CACCAGCACC GTGGACCACG CCCGCCGCCA GCGCGCCCTT
    GAACTGCAGT ACGAGCGTCG TCGCAAGACC CTGGAAGTTC GACGAAAGTC TTGCCAAGAC
    ATGGATGAGA GCCTGATGGC TAGTCCCCAG TCGGATCAAC AGCAGCAGCA ATTGCAGGTC
    ACGCCTTCGT TGTCTGCCTC CGCCACCGCC CTAATGACCA CGCCCAAGAT TCAGTCGACC
    AGCCACCAGA TTAGCCATCG CGCAGAATCT GTGGGACGCA AGTTGGATTT CGGCGGAATG
    AGCCACTCCG CTCAGTCCCT GCTGCGTCGC ACCTCGGAGA GCGATACTTC AACCAGGAGA
    CGACGCACTG TGACGGCGGA CGAAAGAAGA CGCTCCTCCC GCAACCTGGA GAAGTGCATC
    AAACTTATTC CAGCTACCAC TTCCTCGTCG AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG
    GAGATGCGAT CCCTGCTGCA GCAGAGCCGC GACAGGCTGG ACGACACCAG AGCCCTGAAG
    ATCAGATGCC ATCTGCTTAG ACCAGAGGAT TATAACGAGA TCATCAACAC TTGCCGCGAC
    AACATTCGCT GCCTGGAGGC CGTGCTGCGC GGTCCGCCCG GCACCGTGTT GTCCAACCAC
    TGCGCTGGCC AGACAAAAGT ATCAGCCTGT CTCCGCCGGC GCCGGAGGCG ACGTCTAGGG
    ACTGAGCTTC CCGGTCCCAC GGAATCCGGC GATTCCCGGC AGATGGTGCA TATGGTCGAG
    TGCCAGTGTG CGCCCATTAC GCGCTTCGTG GCACGGGTGA TCGAGTTCGT GCTGGACGTG
    GGCAATATGC TGGAGCACTT TGCGCTCTAC CGATTCCTGG CGGGAACGCT GAGGTCGCTC
    TACGAGCTGG TAGACCGGCC CGTGGGTGGA CGCCTCGGTC GGTTCTTTGA CGGGCTGCGG
    GGACTGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17073
    Clone ID Related Accession (Same CDS sequence) NM_001274282.2
    Accession Version NM_001274282.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274282.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGCACCCAA GCCGGTTCAG
    ACCAGGCTCA AAGAACTCAG CACTGAGGAC CATCATAGCA GCATCTCGGA GACGGCGTGG
    GACAACTACC AAGAGAAATA CAACTCGGAG AACTATTCCG AGGGCTTTGA TTCAGATGCG
    GCCCGGCGTC TCTTGGAATT CGGCGACGAC TATCGCAATT TCATCGACTC GCAATCGGAC
    TGCTGCAGTT CTCTGTCGGC GGCCAACAAT CTGGACTCGT TCTCGCCGCC GCGCATGGAC
    TCACTGCAGA AGCACGAGCT GAAGACCCTC CATATCAACC AGGACACAAT CACCAGCACC
    GTGGACCACG CCCGCCGCCA GCGCGCCCTT GAACTGCAGT ACGAGCGTCG TCGCAAGACC
    CTGGAAGTTC GACGAAAGTC TTGCCAAGAC ATGGATGAGA GCCTGATGGC TAGTCCCCAG
    TCGGATCAAC AGCAGCAGCA ATTGCAGGTC ACGCCTTCGT TGTCTGCCTC CGCCACCGCC
    CTAATGACCA CGCCCAAGAT TCAGTCGACC AGCCACCAGA TTAGCCATCG CGCAGAATCT
    GTGGGACGCA AGTTGGATTT CGGCGGAATG AGCCACTCCG CTCAGTCCCT GCTGCGTCGC
    ACCTCGGAGA GCGATACTTC AACCAGGAGA CGACGCACTG TGACGGCGGA CGAAAGAAGA
    CGCTCCTCCC GCAACCTGGA GAAGTGCATC AAACTTATTC CAGCTACCAC TTCCTCGTCG
    AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG GAGATGCGAT CCCTGCTGCA GCAGAGCCGC
    GACAGGCTGG ACGACACCAG AGCCCTGAAG ATCAGATGCC ATCTGCTTAG ACCAGAGGAT
    TATAACGAGA TCATCAACAC TTGCCGCGAC AACATTCGCT GCCTGGAGGC CGTGCTGCGC
    GGTCCGCCCG GCACCGTGTT GTCCAACCAC TGCGCTGGCC AGACAAAAGA TCTGCTCGGC
    GCCTGGGAGG ATCTACTTAG CTGGAGCGAG AATGCCTCCG CTGCCCGCAA ATTGCAGCAG
    GAGATGAGTG TGTTGAAGAG CTCGCTGCAG CGCCTGGGAG ACAAGCCAAC TCCAGAGCTC
    CTCGATACGG AGCCGGCCAT CCAAATAGCA GTGGAGGCGC TCAAGTTGGA GCAGACGCAA
    CTGACCAGCT ATAGGACCAA CATGCTGCGT CTTAACGCCT CCGTCCACAG TTGGCTTACC
    AAGCAGGAGC GGCGACTGCA GAGCGCCTTG GAGGAACAGG AGCAGCAACA GGAGTCCGAA
    CAACTCAAGC AGCAAAAACT TGTTGAAGAG GAGAAGGGAG CTGACGTTCA GAAGGAGCTG
    GCGTCTACCG GAGCAGTGGC CATCACGGTC ACCGACAGCA ATGGTAATCA GGTGGAGGCA
    CTAGCCACAG GAGAAGCCTC CACCTCCACG CCAGCCTGGG ATGTGCACAC GCTGATGTCA
    TCGGAGCAGG AGTTCCATAA GCATCTGAAG AACGAAGTGA GCGACATGTA CAGTTCCTGG
    GATGAGGCGG ATGCCAGAAT CAACACGCAA CTGGAAATGC TTACCAACTC GCTTATTGCC
    TGGAGGCAGT TGGAGTCCGG TTTGAGTGAG TTCCAATTGG CTTTGGGCCA AGATAGGGGC
    ACTCTGAAGG GTCTTGAAGG AGCTCTGGAC AAGGGACAAG CCACACCAGT CGAGCTGGCC
    CAGAATGTAA AACTGGTGGC CAAGCTGCTG TCCGAAAAGG TTCACGTAAG CCAGGAGCAA
    CTTTTGGCCG TCCAGCAGCA CCTGGACCCC AACCACATCT ACCACATTAC GAAGTTTACA
    GCCTCAAATG GCTCGCTATC AGATTCCGGC ATCTCTGATG GAGGAGCTAC TTCTGATGGT
    GGCCTGTCCG AGAGGGAGCG ACGACTGGGA GTTCTTCGAC GCCTAGCAAA GCAGTTGGAA
    CTAGCTCTGG CGCCGGGAAG CGAAGCCATG CGTTCTATTG CTGCCCGAAT GGAAAGTGCC
    GAGGCCGACC TGAAGCACCT GCAGAACACC TGTAGAGACT TAATTGTTCG CACTGCAGCT
    AGCCACCAGC AGAAGCAGCA GATCCAGCAA AATCAGACCC AGCAGGTGTC ACCGAAGGCC
    AATGGACACA TTAAGAAGCA GGCCGCAAAG GGCAAGGCAG AACCCCAGTC GCCTGGCAGA
    CGTGGTAAAG GAGCTCGAAA GGCGCGTCAG GCCAAGAAGG CTGGAGAGGA TCAGCAAGTA
    GAGGAGCCAA GTCTCAGCCC AGAACAGCAG AAGATGGTCC TGAAGCAACT CAAGACCTTG
    ACAAGTGGCG ATGGTGGGGA CGACCCCTCT GACGATCCCT CGTTGCTCTT CAACCTGGAA
    AGCTCCGAGG AAGATGGAGA GGGAGCGGAT CCTGCACAGA CCTCTAAGCG AGGGTGGGCG
    TGGCGCATCG CGAGGGCGGC AGTGCCTATG CAGGTGGCCC TGTTCACAAT CTTCTGCGCT
    GCCTGCCTGA TGCAACCCAA CTGCTGCGAC AATCTGAACA ACCTGTCCAT GAGCTTCACG
    CCGCAGCTCC GCTATATCCG TGGACCACCT CCGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261211.1
    CDS1005..7823
    Translation

    Target ORF information:

    RefSeq Version NM_001274282.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274282.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGCACCCAA GCCGGTTCAG
    ACCAGGCTCA AAGAACTCAG CACTGAGGAC CATCATAGCA GCATCTCGGA GACGGCGTGG
    GACAACTACC AAGAGAAATA CAACTCGGAG AACTATTCCG AGGGCTTTGA TTCAGATGCG
    GCCCGGCGTC TCTTGGAATT CGGCGACGAC TATCGCAATT TCATCGACTC GCAATCGGAC
    TGCTGCAGTT CTCTGTCGGC GGCCAACAAT CTGGACTCGT TCTCGCCGCC GCGCATGGAC
    TCACTGCAGA AGCACGAGCT GAAGACCCTC CATATCAACC AGGACACAAT CACCAGCACC
    GTGGACCACG CCCGCCGCCA GCGCGCCCTT GAACTGCAGT ACGAGCGTCG TCGCAAGACC
    CTGGAAGTTC GACGAAAGTC TTGCCAAGAC ATGGATGAGA GCCTGATGGC TAGTCCCCAG
    TCGGATCAAC AGCAGCAGCA ATTGCAGGTC ACGCCTTCGT TGTCTGCCTC CGCCACCGCC
    CTAATGACCA CGCCCAAGAT TCAGTCGACC AGCCACCAGA TTAGCCATCG CGCAGAATCT
    GTGGGACGCA AGTTGGATTT CGGCGGAATG AGCCACTCCG CTCAGTCCCT GCTGCGTCGC
    ACCTCGGAGA GCGATACTTC AACCAGGAGA CGACGCACTG TGACGGCGGA CGAAAGAAGA
    CGCTCCTCCC GCAACCTGGA GAAGTGCATC AAACTTATTC CAGCTACCAC TTCCTCGTCG
    AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG GAGATGCGAT CCCTGCTGCA GCAGAGCCGC
    GACAGGCTGG ACGACACCAG AGCCCTGAAG ATCAGATGCC ATCTGCTTAG ACCAGAGGAT
    TATAACGAGA TCATCAACAC TTGCCGCGAC AACATTCGCT GCCTGGAGGC CGTGCTGCGC
    GGTCCGCCCG GCACCGTGTT GTCCAACCAC TGCGCTGGCC AGACAAAAGA TCTGCTCGGC
    GCCTGGGAGG ATCTACTTAG CTGGAGCGAG AATGCCTCCG CTGCCCGCAA ATTGCAGCAG
    GAGATGAGTG TGTTGAAGAG CTCGCTGCAG CGCCTGGGAG ACAAGCCAAC TCCAGAGCTC
    CTCGATACGG AGCCGGCCAT CCAAATAGCA GTGGAGGCGC TCAAGTTGGA GCAGACGCAA
    CTGACCAGCT ATAGGACCAA CATGCTGCGT CTTAACGCCT CCGTCCACAG TTGGCTTACC
    AAGCAGGAGC GGCGACTGCA GAGCGCCTTG GAGGAACAGG AGCAGCAACA GGAGTCCGAA
    CAACTCAAGC AGCAAAAACT TGTTGAAGAG GAGAAGGGAG CTGACGTTCA GAAGGAGCTG
    GCGTCTACCG GAGCAGTGGC CATCACGGTC ACCGACAGCA ATGGTAATCA GGTGGAGGCA
    CTAGCCACAG GAGAAGCCTC CACCTCCACG CCAGCCTGGG ATGTGCACAC GCTGATGTCA
    TCGGAGCAGG AGTTCCATAA GCATCTGAAG AACGAAGTGA GCGACATGTA CAGTTCCTGG
    GATGAGGCGG ATGCCAGAAT CAACACGCAA CTGGAAATGC TTACCAACTC GCTTATTGCC
    TGGAGGCAGT TGGAGTCCGG TTTGAGTGAG TTCCAATTGG CTTTGGGCCA AGATAGGGGC
    ACTCTGAAGG GTCTTGAAGG AGCTCTGGAC AAGGGACAAG CCACACCAGT CGAGCTGGCC
    CAGAATGTAA AACTGGTGGC CAAGCTGCTG TCCGAAAAGG TTCACGTAAG CCAGGAGCAA
    CTTTTGGCCG TCCAGCAGCA CCTGGACCCC AACCACATCT ACCACATTAC GAAGTTTACA
    GCCTCAAATG GCTCGCTATC AGATTCCGGC ATCTCTGATG GAGGAGCTAC TTCTGATGGT
    GGCCTGTCCG AGAGGGAGCG ACGACTGGGA GTTCTTCGAC GCCTAGCAAA GCAGTTGGAA
    CTAGCTCTGG CGCCGGGAAG CGAAGCCATG CGTTCTATTG CTGCCCGAAT GGAAAGTGCC
    GAGGCCGACC TGAAGCACCT GCAGAACACC TGTAGAGACT TAATTGTTCG CACTGCAGCT
    AGCCACCAGC AGAAGCAGCA GATCCAGCAA AATCAGACCC AGCAGGTGTC ACCGAAGGCC
    AATGGACACA TTAAGAAGCA GGCCGCAAAG GGCAAGGCAG AACCCCAGTC GCCTGGCAGA
    CGTGGTAAAG GAGCTCGAAA GGCGCGTCAG GCCAAGAAGG CTGGAGAGGA TCAGCAAGTA
    GAGGAGCCAA GTCTCAGCCC AGAACAGCAG AAGATGGTCC TGAAGCAACT CAAGACCTTG
    ACAAGTGGCG ATGGTGGGGA CGACCCCTCT GACGATCCCT CGTTGCTCTT CAACCTGGAA
    AGCTCCGAGG AAGATGGAGA GGGAGCGGAT CCTGCACAGA CCTCTAAGCG AGGGTGGGCG
    TGGCGCATCG CGAGGGCGGC AGTGCCTATG CAGGTGGCCC TGTTCACAAT CTTCTGCGCT
    GCCTGCCTGA TGCAACCCAA CTGCTGCGAC AATCTGAACA ACCTGTCCAT GAGCTTCACG
    CCGCAGCTCC GCTATATCCG TGGACCACCT CCGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20357
    Clone ID Related Accession (Same CDS sequence) NM_001043111.3
    Accession Version NM_001043111.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001043111.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGATAC TGAAATCGGC TGATGCCTCA ACGGATGACG ACGACGAAGG TTGGCTCTAC
    ACGGCGACAG CAGCAGCAAC ATTGGAAGTT GCAACAGCAG CAACTGCAGC ACCGACTGCC
    GCAGCAGCAA CATCGAGTGT GGATGGTCTG CAGTCCACGG CCGTCTCCTC GACTACGGCC
    ACGGGTGGAC CACTCCCGCC CAGCGACGAC TCTGATAAGG AGAACAAGGT GGCTCTGGTC
    AGCGCCACGA CAATCACGGC GGCAGCAACC GCAACTGCAA CATCCATTGC AACACTGCAC
    AGCAGCAACT ACGACAGCAG CAGCGCCTGT TCCTCCTCCA ATTCAAATTC GAATTCGAAC
    TCCAACTCGA ACGGCAGGCT CACGGAGACC TCAGCCACCT CGCGAGTAAC CCAGCTACAG
    ATGCAAATCC ACTCGCAATC GCAGTCGCAG TCACAGATGG AGCTCCAAAT CAATGGCAAT
    GCCATCGACG GCAGACACAT CATTAGCAAC AACAATTGCT ACAGCAGCAT GCAACATCAG
    CCGCAAAACA ACAACGAGGG TGAGGCAGCC GAGGATCTGG CCAAGATCAA GATGGGTGAC
    GATGAAGCAG CTGCTGATAT GGCAAATGGA AACGCCACCA AATCTCAGCA GATGAGCAAC
    GGTGTGTACA GTCGCGCGGA CTCTTGCAAC TTCACCGTTT GGGCGGCAGA AACTGTAGCC
    AGTTGCCACT TGCCCCCGAG ATCGCCGGCC AAATCGGCCA AGTCCTCGAA ATCCCAGGCC
    AGCAATGCCA CCGTGTCTGG ATCCACGCTT GTTTCCCCAG TCAAGGGCAA GGTCTCGCAT
    GATTCAATCA AGCAGTTGGT GCTGGAAGCC GAGCATTTGG TGCGCGACGC CCAAGAGACC
    GCACTGAAGA CGCCAACCAA GCAGAAACAT TCCATCATTA AGATCTCGTC GACGGTCAAG
    AAGCGCGAGG TCACTATGCC CCATCCCATC AAGCAGCGTG TGGAGGAATG GCTGGAGCAC
    CAGCCCTCGA CCCCACAGCT GCTGACCCGG AGCCACACGC ACGAGCTCCT GCCCAGCTGC
    AAGCCAGATG ACTGTGAGGC CTCCGGAGAA GCATCCGAAA CAGACTCGGT GCCCCAAGCT
    GGAGCAGGAG TAAACGGAGG AGCACCGAAT GGTGCGGGAT CGGATACATC GGAGGGCTTC
    ACCGATTCCA TTGCCACATG TATGCAAACG AGCACGAATT CCTATGGGAA TTCCACGGAG
    AGAATCGGTG GATCGGCTGA ACCCATTGGA CAGCCCGTCA CGCCTTTGGG CTTTGGCAGC
    AGCAACCAGA GCCTGAATGT AAAGATTGTA AAGCGATCGC AAACGCGTCG CAAGTCGGAG
    AGACCATGGT CGGTCTCGTG TCTGTCCCAG TTGACTACGG ATGCAGCCCA GTTAACCACG
    GCCCGGATAG TGGAAAACTC TCCGTCCGGT CTGGCCAGTC ACTCGATCAG TGAGAGTGCC
    CTGGACTCGC TTTCGCCAGG ACCAAGACCG CGGGCAGCTT CTTCCTCGGG AACGGGAAGC
    AATGCAGCCA AGAAGGCGGA TAGCAAGGGC TCATTGAGGA GGAGGAAGGC CAGGAAGAAG
    CGCATTTCGG CCGCATCGGC TGGCAGGAAA TCTGATTCAG GTTCCGAACT GGGTGGAGAT
    CTCACCCAGA CACTGATGAA GTCCTGCGAG TCCATGTCCT CGCAGCAGCT GCAGGAGTTC
    ACCAATGCCC TTCTGAGCAT TCAAAAAGGC GCAGTAGTTG CTCCTTTGAG TCCCAAGGGC
    GACGTTTCTG GAGTACCTTC ACTGGCCGAT GGCGAGGGAG GGGAAACCCA GCTGATGCTA
    CCCAAGTTCC GGGTGGGCTC CTTCACCACA GCTGGCCTAT TGGCTACTGA TACGAGGTTA
    GGCGCTTTAG CAGCCCTCTC AAACTACATG AACGAGGACG AACAACAAGC AGAACTCAGC
    ACTGAGGACC ATCATAGCAG CATCTCGGAG ACGGCGTGGG ACAACTACCA AGAGAAATAC
    AACTCGGAGA ACTATTCCGA GGGCTTTGAT TCAGATGCGG CCCGGCGTCT CTTGGAATTC
    GGCGACGACT ATCGCAATTT CATCGACTCG CAATCGGACT GCTGCAGTTC TCTGTCGGCG
    GCCAACAATC TGGACTCGTT CTCGCCGCCG CGCATGGACT CACTGCAGAA GCACGAGCTG
    AAGACCCTCC ATATCAACCA GGACACAATC ACCAGCACCG TGGACCACGC CCGCCGCCAG
    CGCGCCCTTG AACTGCAGTA CGAGCGTCGT CGCAAGACCC TGGAAGTTCG ACGAAAGTCT
    TGCCAAGACA TGGATGAGAG CCTGATGGCT AGTCCCCAGT CGGATCAACA GCAGCAGCAA
    TTGCAGGTCA CGCCTTCGTT GTCTGCCTCC GCCACCGCCC TAATGACCAC GCCCAAGATT
    CAGTCGACCA GCCACCAGAT TAGCCATCGC GCAGAATCTG TGGGACGCAA GTTGGATTTC
    GGCGGAATGA GCCACTCCGC TCAGTCCCTG CTGCGTCGCA CCTCGGAGAG CGATACTTCA
    ACCAGGAGAC GACGCACTGT GACGGCGGAC GAAAGAAGAC GCTCCTCCCG CAACCTGGAG
    AAGTGCATCA AACTTATTCC AGCTACCACT TCCTCGTCGA GTGGCTCGGA CTCCGAGGAC
    GGAGAGCAGG AGATGCGATC CCTGCTGCAG CAGAGCCGCG ACAGGCTGGA CGACACCAGA
    GCCCTGAAGA TCAGATGCCA TCTGCTTAGA CCAGAGGATT ATAACGAGAT CATCAACACT
    TGCCGCGACA ACATTCGCTG CCTGGAGGCC GTGCTGCGCG GTCCGCCCGG CACCGTGTTG
    TCCAACCACT GCGCTGGCCA GACAAAAGAT CTGCTCGGCG CCTGGGAGGA TCTACTTAGC
    TGGAGCGAGA ATGCCTCCGC TGCCCGCAAA TTGCAGCAGG AGATGAGTGT GTTGAAGAGC
    TCGCTGCAGC GCCTGGGAGA CAAGCCAACT CCAGAGCTCC TCGATACGGA GCCGGCCATC
    CAAATAGCAG TGGAGGCGCT CAAGTTGGAG CAGACGCAAC TGACCAGCTA TAGGACCAAC
    ATGCTGCGTC TTAACGCCTC CGTCCACAGT TGGCTTACCA AGCAGGAGCG GCGACTGCAG
    AGCGCCTTGG AGGAACAGGA GCAGCAACAG GAGTCCGAAC AACTCAAGCA GCAAAAACTT
    GTTGAAGAGG AGAAGGGAGC TGACGTTCAG AAGGAGCTGG CGTCTACCGG AGCAGTGGCC
    ATCACGGTCA CCGACAGCAA TGGTAATCAG GTGGAGGCAC TAGCCACAGG AGAAGCCTCC
    ACCTCCACGC CAGCCTGGGA TGTGCACACG CTGATGTCAT CGGAGCAGGA GTTCCATAAG
    CATCTGAAGA ACGAAGTGAG CGACATGTAC AGTTCCTGGG ATGAGGCGGA TGCCAGAATC
    AACACGCAAC TGGAAATGCT TACCAACTCG CTTATTGCCT GGAGGCAGTT GGAGTCCGGT
    TTGAGTGAGT TCCAATTGGC TTTGGGCCAA GATAGGGGCA CTCTGAAGGG TCTTGAAGGA
    GCTCTGGACA AGGGACAAGC CACACCAGTC GAGCTGGCCC AGAATGTAAA ACTGGTGGCC
    AAGCTGCTGT CCGAAAAGGT TCACGTAAGC CAGGAGCAAC TTTTGGCCGT CCAGCAGCAC
    CTGGACCCCA ACCACATCTA CCACATTACG AAGTTTACAG CCTCAAATGG CTCGCTATCA
    GATTCCGGCA TCTCTGATGG AGGAGCTACT TCTGATGGTG GCCTGTCCGA GAGGGAGCGA
    CGACTGGGAG TTCTTCGACG CCTAGCAAAG CAGTTGGAAC TAGCTCTGGC GCCGGGAAGC
    GAAGCCATGC GTTCTATTGC TGCCCGAATG GAAAGTGCCG AGGCCGACCT GAAGCACCTG
    CAGAACACCT GTAGAGACTT AATTGTTCGC ACTGCAGCTA GCCACCAGCA GAAGCAGCAG
    ATCCAGCAAA ATCAGACCCA GCAGGTGTCA CCGAAGGCCA ATGGACACAT TAAGAAGCAG
    GCCGCAAAGG GCAAGGCAGA ACCCCAGTCG CCTGGCAGAC GTGGTAAAGG AGCTCGAAAG
    GCGCGTCAGG CCAAGAAGGC TGGAGAGGAT CAGCAAGTAG AGGAGCCAAG TCTCAGCCCA
    GAACAGCAGA AGATGGTCCT GAAGCAACTC AAGACCTTGA CAAGTGGCGA TGGTGGGGAC
    GACCCCTCTG ACGATCCCTC GTTGCTCTTC AACCTGGAAA GCTCCGAGGA AGATGGAGAG
    GGAGCGGATC CTGCACAGAC CTCTAAGCGA GGGTGGGCGT GGCGCATCGC GAGGGCGGCA
    GTGCCTATGC AGGTGGCCCT GTTCACAATC TTCTGCGCTG CCTGCCTGAT GCAACCCAAC
    TGCTGCGACA ATCTGAACAA CCTGTCCATG AGCTTCACGC CGCAGCTCCG CTATATCCGT
    GGACCACCTC CGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036576.1
    CDS1099..7236
    Translation

    Target ORF information:

    RefSeq Version NM_001043111.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001043111.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGATAC TGAAATCGGC TGATGCCTCA ACGGATGACG ACGACGAAGG TTGGCTCTAC
    ACGGCGACAG CAGCAGCAAC ATTGGAAGTT GCAACAGCAG CAACTGCAGC ACCGACTGCC
    GCAGCAGCAA CATCGAGTGT GGATGGTCTG CAGTCCACGG CCGTCTCCTC GACTACGGCC
    ACGGGTGGAC CACTCCCGCC CAGCGACGAC TCTGATAAGG AGAACAAGGT GGCTCTGGTC
    AGCGCCACGA CAATCACGGC GGCAGCAACC GCAACTGCAA CATCCATTGC AACACTGCAC
    AGCAGCAACT ACGACAGCAG CAGCGCCTGT TCCTCCTCCA ATTCAAATTC GAATTCGAAC
    TCCAACTCGA ACGGCAGGCT CACGGAGACC TCAGCCACCT CGCGAGTAAC CCAGCTACAG
    ATGCAAATCC ACTCGCAATC GCAGTCGCAG TCACAGATGG AGCTCCAAAT CAATGGCAAT
    GCCATCGACG GCAGACACAT CATTAGCAAC AACAATTGCT ACAGCAGCAT GCAACATCAG
    CCGCAAAACA ACAACGAGGG TGAGGCAGCC GAGGATCTGG CCAAGATCAA GATGGGTGAC
    GATGAAGCAG CTGCTGATAT GGCAAATGGA AACGCCACCA AATCTCAGCA GATGAGCAAC
    GGTGTGTACA GTCGCGCGGA CTCTTGCAAC TTCACCGTTT GGGCGGCAGA AACTGTAGCC
    AGTTGCCACT TGCCCCCGAG ATCGCCGGCC AAATCGGCCA AGTCCTCGAA ATCCCAGGCC
    AGCAATGCCA CCGTGTCTGG ATCCACGCTT GTTTCCCCAG TCAAGGGCAA GGTCTCGCAT
    GATTCAATCA AGCAGTTGGT GCTGGAAGCC GAGCATTTGG TGCGCGACGC CCAAGAGACC
    GCACTGAAGA CGCCAACCAA GCAGAAACAT TCCATCATTA AGATCTCGTC GACGGTCAAG
    AAGCGCGAGG TCACTATGCC CCATCCCATC AAGCAGCGTG TGGAGGAATG GCTGGAGCAC
    CAGCCCTCGA CCCCACAGCT GCTGACCCGG AGCCACACGC ACGAGCTCCT GCCCAGCTGC
    AAGCCAGATG ACTGTGAGGC CTCCGGAGAA GCATCCGAAA CAGACTCGGT GCCCCAAGCT
    GGAGCAGGAG TAAACGGAGG AGCACCGAAT GGTGCGGGAT CGGATACATC GGAGGGCTTC
    ACCGATTCCA TTGCCACATG TATGCAAACG AGCACGAATT CCTATGGGAA TTCCACGGAG
    AGAATCGGTG GATCGGCTGA ACCCATTGGA CAGCCCGTCA CGCCTTTGGG CTTTGGCAGC
    AGCAACCAGA GCCTGAATGT AAAGATTGTA AAGCGATCGC AAACGCGTCG CAAGTCGGAG
    AGACCATGGT CGGTCTCGTG TCTGTCCCAG TTGACTACGG ATGCAGCCCA GTTAACCACG
    GCCCGGATAG TGGAAAACTC TCCGTCCGGT CTGGCCAGTC ACTCGATCAG TGAGAGTGCC
    CTGGACTCGC TTTCGCCAGG ACCAAGACCG CGGGCAGCTT CTTCCTCGGG AACGGGAAGC
    AATGCAGCCA AGAAGGCGGA TAGCAAGGGC TCATTGAGGA GGAGGAAGGC CAGGAAGAAG
    CGCATTTCGG CCGCATCGGC TGGCAGGAAA TCTGATTCAG GTTCCGAACT GGGTGGAGAT
    CTCACCCAGA CACTGATGAA GTCCTGCGAG TCCATGTCCT CGCAGCAGCT GCAGGAGTTC
    ACCAATGCCC TTCTGAGCAT TCAAAAAGGC GCAGTAGTTG CTCCTTTGAG TCCCAAGGGC
    GACGTTTCTG GAGTACCTTC ACTGGCCGAT GGCGAGGGAG GGGAAACCCA GCTGATGCTA
    CCCAAGTTCC GGGTGGGCTC CTTCACCACA GCTGGCCTAT TGGCTACTGA TACGAGGTTA
    GGCGCTTTAG CAGCCCTCTC AAACTACATG AACGAGGACG AACAACAAGC AGAACTCAGC
    ACTGAGGACC ATCATAGCAG CATCTCGGAG ACGGCGTGGG ACAACTACCA AGAGAAATAC
    AACTCGGAGA ACTATTCCGA GGGCTTTGAT TCAGATGCGG CCCGGCGTCT CTTGGAATTC
    GGCGACGACT ATCGCAATTT CATCGACTCG CAATCGGACT GCTGCAGTTC TCTGTCGGCG
    GCCAACAATC TGGACTCGTT CTCGCCGCCG CGCATGGACT CACTGCAGAA GCACGAGCTG
    AAGACCCTCC ATATCAACCA GGACACAATC ACCAGCACCG TGGACCACGC CCGCCGCCAG
    CGCGCCCTTG AACTGCAGTA CGAGCGTCGT CGCAAGACCC TGGAAGTTCG ACGAAAGTCT
    TGCCAAGACA TGGATGAGAG CCTGATGGCT AGTCCCCAGT CGGATCAACA GCAGCAGCAA
    TTGCAGGTCA CGCCTTCGTT GTCTGCCTCC GCCACCGCCC TAATGACCAC GCCCAAGATT
    CAGTCGACCA GCCACCAGAT TAGCCATCGC GCAGAATCTG TGGGACGCAA GTTGGATTTC
    GGCGGAATGA GCCACTCCGC TCAGTCCCTG CTGCGTCGCA CCTCGGAGAG CGATACTTCA
    ACCAGGAGAC GACGCACTGT GACGGCGGAC GAAAGAAGAC GCTCCTCCCG CAACCTGGAG
    AAGTGCATCA AACTTATTCC AGCTACCACT TCCTCGTCGA GTGGCTCGGA CTCCGAGGAC
    GGAGAGCAGG AGATGCGATC CCTGCTGCAG CAGAGCCGCG ACAGGCTGGA CGACACCAGA
    GCCCTGAAGA TCAGATGCCA TCTGCTTAGA CCAGAGGATT ATAACGAGAT CATCAACACT
    TGCCGCGACA ACATTCGCTG CCTGGAGGCC GTGCTGCGCG GTCCGCCCGG CACCGTGTTG
    TCCAACCACT GCGCTGGCCA GACAAAAGAT CTGCTCGGCG CCTGGGAGGA TCTACTTAGC
    TGGAGCGAGA ATGCCTCCGC TGCCCGCAAA TTGCAGCAGG AGATGAGTGT GTTGAAGAGC
    TCGCTGCAGC GCCTGGGAGA CAAGCCAACT CCAGAGCTCC TCGATACGGA GCCGGCCATC
    CAAATAGCAG TGGAGGCGCT CAAGTTGGAG CAGACGCAAC TGACCAGCTA TAGGACCAAC
    ATGCTGCGTC TTAACGCCTC CGTCCACAGT TGGCTTACCA AGCAGGAGCG GCGACTGCAG
    AGCGCCTTGG AGGAACAGGA GCAGCAACAG GAGTCCGAAC AACTCAAGCA GCAAAAACTT
    GTTGAAGAGG AGAAGGGAGC TGACGTTCAG AAGGAGCTGG CGTCTACCGG AGCAGTGGCC
    ATCACGGTCA CCGACAGCAA TGGTAATCAG GTGGAGGCAC TAGCCACAGG AGAAGCCTCC
    ACCTCCACGC CAGCCTGGGA TGTGCACACG CTGATGTCAT CGGAGCAGGA GTTCCATAAG
    CATCTGAAGA ACGAAGTGAG CGACATGTAC AGTTCCTGGG ATGAGGCGGA TGCCAGAATC
    AACACGCAAC TGGAAATGCT TACCAACTCG CTTATTGCCT GGAGGCAGTT GGAGTCCGGT
    TTGAGTGAGT TCCAATTGGC TTTGGGCCAA GATAGGGGCA CTCTGAAGGG TCTTGAAGGA
    GCTCTGGACA AGGGACAAGC CACACCAGTC GAGCTGGCCC AGAATGTAAA ACTGGTGGCC
    AAGCTGCTGT CCGAAAAGGT TCACGTAAGC CAGGAGCAAC TTTTGGCCGT CCAGCAGCAC
    CTGGACCCCA ACCACATCTA CCACATTACG AAGTTTACAG CCTCAAATGG CTCGCTATCA
    GATTCCGGCA TCTCTGATGG AGGAGCTACT TCTGATGGTG GCCTGTCCGA GAGGGAGCGA
    CGACTGGGAG TTCTTCGACG CCTAGCAAAG CAGTTGGAAC TAGCTCTGGC GCCGGGAAGC
    GAAGCCATGC GTTCTATTGC TGCCCGAATG GAAAGTGCCG AGGCCGACCT GAAGCACCTG
    CAGAACACCT GTAGAGACTT AATTGTTCGC ACTGCAGCTA GCCACCAGCA GAAGCAGCAG
    ATCCAGCAAA ATCAGACCCA GCAGGTGTCA CCGAAGGCCA ATGGACACAT TAAGAAGCAG
    GCCGCAAAGG GCAAGGCAGA ACCCCAGTCG CCTGGCAGAC GTGGTAAAGG AGCTCGAAAG
    GCGCGTCAGG CCAAGAAGGC TGGAGAGGAT CAGCAAGTAG AGGAGCCAAG TCTCAGCCCA
    GAACAGCAGA AGATGGTCCT GAAGCAACTC AAGACCTTGA CAAGTGGCGA TGGTGGGGAC
    GACCCCTCTG ACGATCCCTC GTTGCTCTTC AACCTGGAAA GCTCCGAGGA AGATGGAGAG
    GGAGCGGATC CTGCACAGAC CTCTAAGCGA GGGTGGGCGT GGCGCATCGC GAGGGCGGCA
    GTGCCTATGC AGGTGGCCCT GTTCACAATC TTCTGCGCTG CCTGCCTGAT GCAACCCAAC
    TGCTGCGACA ATCTGAACAA CCTGTCCATG AGCTTCACGC CGCAGCTCCG CTATATCCGT
    GGACCACCTC CGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa20358
    Clone ID Related Accession (Same CDS sequence) NM_079149.5
    Accession Version NM_079149.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product klarsicht, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079149.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGAACTCAG CACTGAGGAC
    CATCATAGCA GCATCTCGGA GACGGCGTGG GACAACTACC AAGAGAAATA CAACTCGGAG
    AACTATTCCG AGGGCTTTGA TTCAGATGCG GCCCGGCGTC TCTTGGAATT CGGCGACGAC
    TATCGCAATT TCATCGACTC GCAATCGGAC TGCTGCAGTT CTCTGTCGGC GGCCAACAAT
    CTGGACTCGT TCTCGCCGCC GCGCATGGAC TCACTGCAGA AGCACGAGCT GAAGACCCTC
    CATATCAACC AGGACACAAT CACCAGCACC GTGGACCACG CCCGCCGCCA GCGCGCCCTT
    GAACTGCAGT ACGAGCGTCG TCGCAAGACC CTGGAAGTTC GACGAAAGTC TTGCCAAGAC
    ATGGATGAGA GCCTGATGGC TAGTCCCCAG TCGGATCAAC AGCAGCAGCA ATTGCAGGTC
    ACGCCTTCGT TGTCTGCCTC CGCCACCGCC CTAATGACCA CGCCCAAGAT TCAGTCGACC
    AGCCACCAGA TTAGCCATCG CGCAGAATCT GTGGGACGCA AGTTGGATTT CGGCGGAATG
    AGCCACTCCG CTCAGTCCCT GCTGCGTCGC ACCTCGGAGA GCGATACTTC AACCAGGAGA
    CGACGCACTG TGACGGCGGA CGAAAGAAGA CGCTCCTCCC GCAACCTGGA GAAGTGCATC
    AAACTTATTC CAGCTACCAC TTCCTCGTCG AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG
    GAGATGCGAT CCCTGCTGCA GCAGAGCCGC GACAGGCTGG ACGACACCAG AGCCCTGAAG
    ATCAGATGCC ATCTGCTTAG ACCAGAGGAT TATAACGAGA TCATCAACAC TTGCCGCGAC
    AACATTCGCT GCCTGGAGGC CGTGCTGCGC GGTCCGCCCG GCACCGTGTT GTCCAACCAC
    TGCGCTGGCC AGACAAAAGA TCTGCTCGGC GCCTGGGAGG ATCTACTTAG CTGGAGCGAG
    AATGCCTCCG CTGCCCGCAA ATTGCAGCAG GAGATGAGTG TGTTGAAGAG CTCGCTGCAG
    CGCCTGGGAG ACAAGCCAAC TCCAGAGCTC CTCGATACGG AGCCGGCCAT CCAAATAGCA
    GTGGAGGCGC TCAAGTTGGA GCAGACGCAA CTGACCAGCT ATAGGACCAA CATGCTGCGT
    CTTAACGCCT CCGTCCACAG TTGGCTTACC AAGCAGGAGC GGCGACTGCA GAGCGCCTTG
    GAGGAACAGG AGCAGCAACA GGAGTCCGAA CAACTCAAGC AGCAAAAACT TGTTGAAGAG
    GAGAAGGGAG CTGACGTTCA GAAGGAGCTG GCGTCTACCG GAGCAGTGGC CATCACGGTC
    ACCGACAGCA ATGGTAATCA GGTGGAGGCA CTAGCCACAG GAGAAGCCTC CACCTCCACG
    CCAGCCTGGG ATGTGCACAC GCTGATGTCA TCGGAGCAGG AGTTCCATAA GCATCTGAAG
    AACGAAGTGA GCGACATGTA CAGTTCCTGG GATGAGGCGG ATGCCAGAAT CAACACGCAA
    CTGGAAATGC TTACCAACTC GCTTATTGCC TGGAGGCAGT TGGAGTCCGG TTTGAGTGAG
    TTCCAATTGG CTTTGGGCCA AGATAGGGGC ACTCTGAAGG GTCTTGAAGG AGCTCTGGAC
    AAGGGACAAG CCACACCAGT CGAGCTGGCC CAGAATGTAA AACTGGTGGC CAAGCTGCTG
    TCCGAAAAGG TTCACGTAAG CCAGGAGCAA CTTTTGGCCG TCCAGCAGCA CCTGGACCCC
    AACCACATCT ACCACATTAC GAAGTTTACA GCCTCAAATG GCTCGCTATC AGATTCCGGC
    ATCTCTGATG GAGGAGCTAC TTCTGATGGT GGCCTGTCCG AGAGGGAGCG ACGACTGGGA
    GTTCTTCGAC GCCTAGCAAA GCAGTTGGAA CTAGCTCTGG CGCCGGGAAG CGAAGCCATG
    CGTTCTATTG CTGCCCGAAT GGAAAGTGCC GAGGCCGACC TGAAGCACCT GCAGAACACC
    TGTAGAGACT TAATTGTTCG CACTGCAGCT AGCCACCAGC AGAAGCAGCA GATCCAGCAA
    AATCAGACCC AGCAGGTGTC ACCGAAGGCC AATGGACACA TTAAGAAGCA GGCCGCAAAG
    GGCAAGGCAG AACCCCAGTC GCCTGGCAGA CGTGGTAAAG GAGCTCGAAA GGCGCGTCAG
    GCCAAGAAGG CTGGAGAGGA TCAGCAAGTA GAGGAGCCAA GTCTCAGCCC AGAACAGCAG
    AAGATGGTCC TGAAGCAACT CAAGACCTTG ACAAGTGGCG ATGGTGGGGA CGACCCCTCT
    GACGATCCCT CGTTGCTCTT CAACCTGGAA AGCTCCGAGG AAGATGGAGA GGGAGCGGAT
    CCTGCACAGA CCTCTAAGCG AGGGTGGGCG TGGCGCATCG CGAGGGCGGC AGTGCCTATG
    CAGGTGGCCC TGTTCACAAT CTTCTGCGCT GCCTGCCTGA TGCAACCCAA CTGCTGCGAC
    AATCTGAACA ACCTGTCCAT GAGCTTCACG CCGCAGCTCC GCTATATCCG TGGACCACCT
    CCGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523873.2
    CDS1099..7887
    Translation

    Target ORF information:

    RefSeq Version NM_079149.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster klarsicht (klar), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079149.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAATGC AACAGGAAAA CGAGACGGGA AGAGAAGGGG TAATCCCGAA CTCGGAGAAA 
    GAAGTAGCGT TGATAAGTAA CCAAGAGACT GAAAAAGCGG CCATGATTAG TGGTGGCAAA
    GAAGGTTCGG TAAGGGATTT GGAGTCCCCA AACGAAACCG CCACCACCCA AAAAATAGAA
    CACACAACGA AACCACTTAA ATTGGATGGC ATTTTTGCCA TGCCAGCCAC TCCGCCGAAT
    AGGGCCGGAC TAACAACAAA ATCGGCCAGC AGTAGCCGTA GCAGTTCCCT CAAAAAGCAG
    AGACGAGGAT CCAGAGGAAG TGCCAATTTG AATGTAGCGG GAACGGGTGA TGGAGCACCC
    AAAAGAAGCG CCCCCGGCGG TGGTCAGCTA AACTATGGCA CCAATAGTGT TGGAAACGAC
    CTTATGAAGC AATCGCAGTC CATGACGTCA CTGAAGGCCT CTAATGAGGA ACAGCAGGAG
    AGTCGTGACG TCATCCAGGC CAAACTTCAC TTGGAACGTC CCTACAACAG CCTCAAGAAA
    AATTCGCATA GCAATAAAAT GCACCGTTAT TCGTGGGGAA GCGGAAACAG TCAATGCAGT
    TCCACCAGTC TGCACAGCAG CGCCACCTTG GGTCTGGGTT CGTCTGGCAA AGACGACCTC
    TGGGCGGCCA TTCAAACGAA CTACAACTAC ATTATGGACA CGAACCTTTT GGACACTTGC
    AAGGAGGCGC GCTGTGAGAT AGAGGGCGCT GCCACGGTGT TGGAGAAATC CAGTGAATGT
    TCCTTTAAGA TGCTCGATGA GACCCAACGA CCGGAGGGTC TTTGTGAGGA TCCCAAGGAG
    TTGCGGCGTT GGCTGCGCGA AATGGAGAAT AAATTGGAGA GTTCGCCGAC AATCACAGAG
    CTGACTTTGT TTGGCAACGC GGAATTGCAG CGCCATCTAG CAGTACACTC GGTGTTGTAC
    CATGAAATTG CATCACACGC TCGAGTGGTG AGCTCTTGCA TTCGAGCCGC CGAAAAGGAA
    CAGCAACTCC AACAGCAGCA GCAACTGTCG AGTCAGCAAC CCGCCTCATT GACCAGCAAC
    TGCTCCAGTG AGTCAACCAG CGAATCGGCC ACAAAGTCGA GCAGCCTGAG CAGTGGTTTC
    GCTTCCGATC CGGTGACCAC ACCCATTGGA ACCGCAGCAG CGGCCCCGCC GTCAAGTAGC
    ACCCACCCAT CCAAGAAGGG GGAGGGCAAC TTGGAGCGAC TTAGGGATCG CTACCATCTG
    TTGTATCTGA AGGCGTTCGA ACTGCAATTG TGCCTGGATA ATCTGCTCAG GAAACGCAGT
    TCCGCAGCGA ATGGTTTGGA TGACGACGAG GACGAAGAGG AGGACACCGA AGACGATTCC
    TTTGGCTACG AGGGCGAAGC CACCGAGGAC GATCTGAACG AGGTCAACTC GGATCTCGAT
    CTAGAGAACA GCGAGTCCAA GTCGGCCACC CCCGCGGAAC TTATTCTCCA GCGCTGTCAG
    ATTGCAGCTA CCCAGATTGT GTGCGGACTG GATGAGACCC AATCCCAGTC GCGGTCACAG
    CAAGTCCCAT CCCAAGCGAA GTCGCCGTCA GCGGATCAGC CACGCGATTG TCTTGCACCT
    CAAAAGCCCG GCGATGAGGC CGATGAAGAG CTAGAGGAGG AGGACGAGGA TCCTGATATC
    GGCACCGATG TGGTGGACTT CCTGATTGCC AAGCGATCGT GGAGACAGGT GAACCACCCG
    AATCCCAGTG GTACAGCCAC CCTAACGGAT TTCGAGGCGG ATTCAGAGAG CAGTGACTTC
    GAGCAGGTGC AGCAGTTGAG CAGAAGAGGA TTACCCCCAA CAGTGGGCAG CACCCGAAGG
    GTCCTTAACC TCATTGAGAT GCCCCAGAGC AGCAACAGCA ATATCAGCAG CAGCAACTCG
    TTGCTACAGC AACGAAATCA CAATATCGGC AACAAAATGC TGCCCAACAA AGCGCACGGC
    AAGAACATTG CCGTGGCGGT AATCACACCC AATTCACATG GAAACACCAG TCACGGTCAT
    GGGCTCGGTC ACGGACTCGG CCACGAGCTG AACAAGTCGC CTTTGGGGCT GCGCAAGACG
    CGTCATCACC ACAATGACAC CTCCAAGTTC AACCGATCCA ATCGCAAGTC GAAGAACTGT
    GCGATCTTCT ATTTCAAGCA CCTGGACACG GACAACGAGC AGGGAAACGC GGCGGGCAGT
    GATCTCCAGA GCGAGGATGA TCCGAGCCTG ATCCATCGGC GCAGAGCTGG AGGCGACATA
    CTGAAATCGG CTGATGCCTC AACGGATGAC GACGACGAAG GTTGGCTCTA CACGGCGACA
    GCAGCAGCAA CATTGGAAGT TGCAACAGCA GCAACTGCAG CACCGACTGC CGCAGCAGCA
    ACATCGAGTG TGGATGGTCT GCAGTCCACG GCCGTCTCCT CGACTACGGC CACGGGTGGA
    CCACTCCCGC CCAGCGACGA CTCTGATAAG GAGAACAAGG TGGCTCTGGT CAGCGCCACG
    ACAATCACGG CGGCAGCAAC CGCAACTGCA ACATCCATTG CAACACTGCA CAGCAGCAAC
    TACGACAGCA GCAGCGCCTG TTCCTCCTCC AATTCAAATT CGAATTCGAA CTCCAACTCG
    AACGGCAGGC TCACGGAGAC CTCAGCCACC TCGCGAGTAA CCCAGCTACA GATGCAAATC
    CACTCGCAAT CGCAGTCGCA GTCACAGATG GAGCTCCAAA TCAATGGCAA TGCCATCGAC
    GGCAGACACA TCATTAGCAA CAACAATTGC TACAGCAGCA TGCAACATCA GCCGCAAAAC
    AACAACGAGG GTGAGGCAGC CGAGGATCTG GCCAAGATCA AGATGGGTGA CGATGAAGCA
    GCTGCTGATA TGGCAAATGG AAACGCCACC AAATCTCAGC AGATGAGCAA CGGTGTGTAC
    AGTCGCGCGG ACTCTTGCAA CTTCACCGTT TGGGCGGCAG AAACTGTAGC CAGTTGCCAC
    TTGCCCCCGA GATCGCCGGC CAAATCGGCC AAGTCCTCGA AATCCCAGGC CAGCAATGCC
    ACCGTGTCTG GATCCACGCT TGTTTCCCCA GTCAAGGGCA AGGTCTCGCA TGATTCAATC
    AAGCAGTTGG TGCTGGAAGC CGAGCATTTG GTGCGCGACG CCCAAGAGAC CGCACTGAAG
    ACGCCAACCA AGCAGAAACA TTCCATCATT AAGATCTCGT CGACGGTCAA GAAGCGCGAG
    GTCACTATGC CCCATCCCAT CAAGCAGCGT GTGGAGGAAT GGCTGGAGCA CCAGCCCTCG
    ACCCCACAGC TGCTGACCCG GAGCCACACG CACGAGCTCC TGCCCAGCTG CAAGCCAGAT
    GACTGTGAGG CCTCCGGAGA AGCATCCGAA ACAGACTCGG TGCCCCAAGC TGGAGCAGGA
    GTAAACGGAG GAGCACCGAA TGGTGCGGGA TCGGATACAT CGGAGGGCTT CACCGATTCC
    ATTGCCACAT GTATGCAAAC GAGCACGAAT TCCTATGGGA ATTCCACGGA GAGAATCGGT
    GGATCGGCTG AACCCATTGG ACAGCCCGTC ACGCCTTTGG GCTTTGGCAG CAGCAACCAG
    AGCCTGAATG TAAAGATTGT AAAGCGATCG CAAACGCGTC GCAAGTCGGA GAGACCATGG
    TCGGTCTCGT GTCTGTCCCA GTTGACTACG GATGCAGCCC AGTTAACCAC GGCCCGGATA
    GTGGAAAACT CTCCGTCCGG TCTGGCCAGT CACTCGATCA GTGAGAGTGC CCTGGACTCG
    CTTTCGCCAG GACCAAGACC GCGGGCAGCT TCTTCCTCGG GAACGGGAAG CAATGCAGCC
    AAGAAGGCGG ATAGCAAGGG CTCATTGAGG AGGAGGAAGG CCAGGAAGAA GCGCATTTCG
    GCCGCATCGG CTGGCAGGAA ATCTGATTCA GGTTCCGAAC TGGGTGGAGA TCTCACCCAG
    ACACTGATGA AGTCCTGCGA GTCCATGTCC TCGCAGCAGC TGCAGGAGTT CACCAATGCC
    CTTCTGAGCA TTCAAAAAGG CGCAGTAGTT GCTCCTTTGA GTCCCAAGGG CGACGTTTCT
    GGAGTACCTT CACTGGCCGA TGGCGAGGGA GGGGAAACCC AGCTGATGCT ACCCAAGTTC
    CGGGTGGGCT CCTTCACCAC AGCTGGCCTA TTGGCTACTG ATACGAGGTT AGGCGCTTTA
    GCAGCCCTCT CAAACTACAT GAACGAGGAC GAACAACAAG CAGAACTCAG CACTGAGGAC
    CATCATAGCA GCATCTCGGA GACGGCGTGG GACAACTACC AAGAGAAATA CAACTCGGAG
    AACTATTCCG AGGGCTTTGA TTCAGATGCG GCCCGGCGTC TCTTGGAATT CGGCGACGAC
    TATCGCAATT TCATCGACTC GCAATCGGAC TGCTGCAGTT CTCTGTCGGC GGCCAACAAT
    CTGGACTCGT TCTCGCCGCC GCGCATGGAC TCACTGCAGA AGCACGAGCT GAAGACCCTC
    CATATCAACC AGGACACAAT CACCAGCACC GTGGACCACG CCCGCCGCCA GCGCGCCCTT
    GAACTGCAGT ACGAGCGTCG TCGCAAGACC CTGGAAGTTC GACGAAAGTC TTGCCAAGAC
    ATGGATGAGA GCCTGATGGC TAGTCCCCAG TCGGATCAAC AGCAGCAGCA ATTGCAGGTC
    ACGCCTTCGT TGTCTGCCTC CGCCACCGCC CTAATGACCA CGCCCAAGAT TCAGTCGACC
    AGCCACCAGA TTAGCCATCG CGCAGAATCT GTGGGACGCA AGTTGGATTT CGGCGGAATG
    AGCCACTCCG CTCAGTCCCT GCTGCGTCGC ACCTCGGAGA GCGATACTTC AACCAGGAGA
    CGACGCACTG TGACGGCGGA CGAAAGAAGA CGCTCCTCCC GCAACCTGGA GAAGTGCATC
    AAACTTATTC CAGCTACCAC TTCCTCGTCG AGTGGCTCGG ACTCCGAGGA CGGAGAGCAG
    GAGATGCGAT CCCTGCTGCA GCAGAGCCGC GACAGGCTGG ACGACACCAG AGCCCTGAAG
    ATCAGATGCC ATCTGCTTAG ACCAGAGGAT TATAACGAGA TCATCAACAC TTGCCGCGAC
    AACATTCGCT GCCTGGAGGC CGTGCTGCGC GGTCCGCCCG GCACCGTGTT GTCCAACCAC
    TGCGCTGGCC AGACAAAAGA TCTGCTCGGC GCCTGGGAGG ATCTACTTAG CTGGAGCGAG
    AATGCCTCCG CTGCCCGCAA ATTGCAGCAG GAGATGAGTG TGTTGAAGAG CTCGCTGCAG
    CGCCTGGGAG ACAAGCCAAC TCCAGAGCTC CTCGATACGG AGCCGGCCAT CCAAATAGCA
    GTGGAGGCGC TCAAGTTGGA GCAGACGCAA CTGACCAGCT ATAGGACCAA CATGCTGCGT
    CTTAACGCCT CCGTCCACAG TTGGCTTACC AAGCAGGAGC GGCGACTGCA GAGCGCCTTG
    GAGGAACAGG AGCAGCAACA GGAGTCCGAA CAACTCAAGC AGCAAAAACT TGTTGAAGAG
    GAGAAGGGAG CTGACGTTCA GAAGGAGCTG GCGTCTACCG GAGCAGTGGC CATCACGGTC
    ACCGACAGCA ATGGTAATCA GGTGGAGGCA CTAGCCACAG GAGAAGCCTC CACCTCCACG
    CCAGCCTGGG ATGTGCACAC GCTGATGTCA TCGGAGCAGG AGTTCCATAA GCATCTGAAG
    AACGAAGTGA GCGACATGTA CAGTTCCTGG GATGAGGCGG ATGCCAGAAT CAACACGCAA
    CTGGAAATGC TTACCAACTC GCTTATTGCC TGGAGGCAGT TGGAGTCCGG TTTGAGTGAG
    TTCCAATTGG CTTTGGGCCA AGATAGGGGC ACTCTGAAGG GTCTTGAAGG AGCTCTGGAC
    AAGGGACAAG CCACACCAGT CGAGCTGGCC CAGAATGTAA AACTGGTGGC CAAGCTGCTG
    TCCGAAAAGG TTCACGTAAG CCAGGAGCAA CTTTTGGCCG TCCAGCAGCA CCTGGACCCC
    AACCACATCT ACCACATTAC GAAGTTTACA GCCTCAAATG GCTCGCTATC AGATTCCGGC
    ATCTCTGATG GAGGAGCTAC TTCTGATGGT GGCCTGTCCG AGAGGGAGCG ACGACTGGGA
    GTTCTTCGAC GCCTAGCAAA GCAGTTGGAA CTAGCTCTGG CGCCGGGAAG CGAAGCCATG
    CGTTCTATTG CTGCCCGAAT GGAAAGTGCC GAGGCCGACC TGAAGCACCT GCAGAACACC
    TGTAGAGACT TAATTGTTCG CACTGCAGCT AGCCACCAGC AGAAGCAGCA GATCCAGCAA
    AATCAGACCC AGCAGGTGTC ACCGAAGGCC AATGGACACA TTAAGAAGCA GGCCGCAAAG
    GGCAAGGCAG AACCCCAGTC GCCTGGCAGA CGTGGTAAAG GAGCTCGAAA GGCGCGTCAG
    GCCAAGAAGG CTGGAGAGGA TCAGCAAGTA GAGGAGCCAA GTCTCAGCCC AGAACAGCAG
    AAGATGGTCC TGAAGCAACT CAAGACCTTG ACAAGTGGCG ATGGTGGGGA CGACCCCTCT
    GACGATCCCT CGTTGCTCTT CAACCTGGAA AGCTCCGAGG AAGATGGAGA GGGAGCGGAT
    CCTGCACAGA CCTCTAAGCG AGGGTGGGCG TGGCGCATCG CGAGGGCGGC AGTGCCTATG
    CAGGTGGCCC TGTTCACAAT CTTCTGCGCT GCCTGCCTGA TGCAACCCAA CTGCTGCGAC
    AATCTGAACA ACCTGTCCAT GAGCTTCACG CCGCAGCTCC GCTATATCCG TGGACCACCT
    CCGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.