Dis3l2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dis3l2
Entrez Gene ID	38034
Full Name	CG16940 gene product from transcript CG16940-RA
Synonyms	Dmel\CG16940
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B2-61B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_167805.2	NP_728490.1	Dis3 like 3'-5' exoribonuclease 2, isoform C
NM_138168.3	NP_612012.1	Dis3 like 3'-5' exoribonuclease 2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mutants of Tailor and Dis3L2 are viable and lack overt morphological defects. Instead, these mutants exhibit defects in female and male fertility, implying specific requirements in the germline.
Title: Characterization of a TUTase/RNase complex required for

Here, the authors show that in flies, 3' terminal RNA uridylation triggers the processive, 3'-to-5' exoribonucleolytic decay via the RNase II/R enzyme CG16940, a homolog of the human Perlman syndrome exoribonuclease Dis3l2.
Title: Molecular basis for cytoplasmic RNA surveillance by uridylation-triggered decay in Drosophila.

The following Dis3l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dis3l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17044	NM_138168.3 Latest version!	Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17045	NM_167805.2 Latest version!	Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dis3l2 ( NM_138168.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612012.1 Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17044
Clone ID Related Accession (Same CDS sequence)	NM_138168.3
Accession Version	NM_138168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dis3 like 3'-5' exoribonuclease 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_138168.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAA CGTCAAGCGT CAACGTGGAT GCGGACCGAG ATTGCAGAAG GATTGAGGGC 
CTCCGACAGC GCTCACAACG CCTTGCTGCC CGCCTCCAGC AGATGCAGAG GGAAATGGAG 
TCTAATGCAT CCAGCAAAGA CCTATCGGCC AACAATGCCG TTATTCCGCT GCAGCCACTA 
ATGCGCTGGG CGGAACCACG GCCACATCCC AACCAATCCC AACCATCTGC TCGGAGCAAC 
AGCAGGCAGG CCATTCCTTA CGGTGTGCCA GTATCCACGC CAGCGCCGGT AGCTATGCAA 
TTGCCCTACC ACGTAGCATT CGCCTCCTAC CAGGCGACCA TGCAGATTCC CTGCCTTCAG 
CCACTTTCGC ATTTGCACGT CGTGTCTAGT CCCAGCCATC GGTTAATAGC GAAAAAGAAG 
TCGAAGCGGG CTCTTCGAAA TGAGGTGGAA GCTCTTCAGG ATATGGTCAA ACATCTTTCA 
CACCTCTCCC CAGATGTTAA CGCGTATGCC TGCCAGCTGA TACGTAAAAA CGAGATCCTT 
TCTCAGCTCG AGTGGAGTCA GACCAGGTCA GAAGCACATC CGCAACAGGT TGGTTACCAG 
GGGCGGCATC GCCACGATTC TGAGGTCTCT GCTACAAACA ATGGAAGTCA TGTACAGCAG 
CAACCGAAAC TAGGACGAAG GCAACGCAAT AGCAAGAAAA ACGATGGATC CGGTAGCAAT 
GGGACCCAAT CGCAAAAAAC CGAACTAGAG GCCATGCGGT CGTACATAAA CAAGATTGTC 
CAACGTCACG TGGGTATGGA TCACCAGTTG TCGGAAGACT TGATTTTCAA AGGTAACTTC 
TCCGACCTGG AAGCGCATGC GCAGAGACTA GTTGCGGCGG GTTATGGGCG CATTGTAGAA 
GAGGAAATCC GGGTTAACCG CAAGAACAAC AGACAGGCCT TTATAATCAT GTCCACTGAT 
CGAGAAGCAC TCGAACGAGA TGGCATCGTG CTCTTGCCAG TGGCTAGGCG CTATGCCTTC 
GATGGTGACA AAGTACGTGC ATTCGTGCTA AACCCTGGTG CTCAGGGTAG TTCAAAGACA 
GCTGAGCCCT CGTCTGGTGA AATCTCTGGT GGGAAGCCCT CATTATCTCT TGCGGATGGT 
GAGGAGCTTT CGGACGATAC CGAGTCCCAG GGCTCTGAGT CCGATACCGA CAACGTGGTC 
GTGATATCGT CGGACAACTG CCCTAAAGCG TTCGTCATAG CAATAACTAA ACGAACTGAG 
CTCCGCCAGA TCGTGGGAAC CATATCGTTT ACGAACCCAA CCAAACTCTG TGACGACCAG 
CTATTCTACA AATTCCGACC GTACGATTTG CGTGTCCCCA TGGTCTACGT TCCGAAGGAT 
GCCTGTGCCG CCCACATCGG AAACAAGCAG CAAATAGACG TGTCCGGCCT CTTATACCTT 
GCCCATATAC TCGAGACAGA TTGCAATGGG CACTGCATCG CTGAACTAAT CCAGCCGGTT 
GGCCGCGTGG GTAATTTGGA TGACGAACTT AAGGCAATTC TGTTTCATAA CGGTCTCCGG 
GATATAAAGC CATTTGAGCA ACGATTTATC GACATTTACT CCCAGCCGCC ACCTCCGATA 
AGCCAAGAGG ATCTGCGTCA ACGAAAAGAC TTAAGAAAGA TGTGCATATT TACTATAGAT 
CCGATGACTG CTCGCGATTT GGATGACGCC GTTTCTATAG AGAAGCTAGG CGACAACGAA 
TATGAGATTG GCGTCCATAT ATCCGATGTT TCGCACTTCC TGATAGAGGA CAATGAACTG 
GATAACATAG TGAAGGAACG CTCTACGTCT ATTTATTTAG CCAACGAGGT AATCCACATG 
CTGCCTCAGT CTCTATGCAT GCGATGTTCC CTGCTCCCTG GGCAAGACAA GTTTGCTTTT 
TCCGTCTTCT GGCGGATGAA CGGGAAGGGA GTCATGCTGC AAAAGAAGCC AGAATTTTGC 
CGCACAGTCA TTAACTCTTG CTCGCAATTT GCTTATGAAC ACGCTCAAAA GATCATCGAC 
AACCCTAATG AACGGTTTAC CGAGAACGAC TTTCCGACCA TCCTGAATGG ATTTAATCCC 
GATGATATCA GAAATAGGGT GCTATGGCTG CACGACATTG CAAGCTCCAT CCGTAAGACA 
CGTTTAGACA ATGGCGCACT CACAATTAAC AATGCCAAGC TACGCTTCCT GCTCGATCCA 
ATTACTGGCG AGCCGTTGTC TTTTGAAGTG GAGAAACAGC GAGAGGCTAA CCGGCTGATT 
GAAGAGTTTA TGCTCCTGGC CAACCAGGCA GTCGCCCGCT TTATACACGA CTCCTTTCCG 
GATATCGCTG TACTGCGTAA CCACCCTCCG CCACTTATTA AGTCCCTTAA GGCTTTAAGG 
GAAAAGCTGC TTGCTCTGGG ATTCGAATTG GACTATAGCT CGTCTAAGGC GCTCCAGGAG 
AGCATGGTGC GGTTGTGCAA TGAGGCGCCC AATCCTGTCG CAATGAATGC CTGCCTAAGT 
CAGCTTTTGA TGAAACCAAT GGCCCGTGCA ACATATTTTT GTAGCGAGGG TAAATCGGAA 
CCGGCTGACC TGTGGCACTA CGCCTTGTCC ATACCCATAT ACACTCACTT TACTAGCCCA 
ATCCGTCGGT ATCCTGATAT TTTGGTGCAC CGACTTTTGG CGGCCGCACT TAAATATTGT 
ACACCGCCTA AACGTACCCC AGATGATTTG CACACCTTAA CGAAATTAGC GAATGAGCGC 
AAGTACAATG CGAAAAAGGC CGGAGAGGAC TCTGGTAACT TGTACTTCAA ACGATATGTT 
CACAACAAGC AGGGCATATA CATGCGAGCT GTGGTCATTG AAATATTTCA GCATATGATG 
AACGTAGTTA CTCTGGAGTC GGGCCACGTT ATAAGCATTA ACTACAAAAT GCAAAAGGTC 
CTCGTAGACA CACACGGCGT ACCCAATTAC ATTCTTATTG CCGAGCGCAA CTTAAAGCAG 
TCACCTAGGA AGTTGCAGCT GCTTTCCGTG GTCCCCATAT GTCTGATCAT TTGGGACCAA 
AAACTAACTG GATTTTTAAA GATGGAAGAA CAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612012.1
CDS	137..3235
Translation	MSETSSVNVDADRDCRRIEGLRQRSQRLAARLQQMQREMESNASSKDLSANNAVIPLQPLMRWAEPRPHPNQSQPSARSNSRQAIPYGVPVSTPAPVAMQLPYHVAFASYQATMQIPCLQPLSHLHVVSSPSHRLIAKKKSKRALRNEVEALQDMVKHLSHLSPDVNAYACQLIRKNEILSQLEWSQTRSEAHPQQVGYQGRHRHDSEVSATNNGSHVQQQPKLGRRQRNSKKNDGSGSNGTQSQKTELEAMRSYINKIVQRHVGMDHQLSEDLIFKGNFSDLEAHAQRLVAAGYGRIVEEEIRVNRKNNRQAFIIMSTDREALERDGIVLLPVARRYAFDGDKVRAFVLNPGAQGSSKTAEPSSGEISGGKPSLSLADGEELSDDTESQGSESDTDNVVVISSDNCPKAFVIAITKRTELRQIVGTISFTNPTKLCDDQLFYKFRPYDLRVPMVYVPKDACAAHIGNKQQIDVSGLLYLAHILETDCNGHCIAELIQPVGRVGNLDDELKAILFHNGLRDIKPFEQRFIDIYSQPPPPISQEDLRQRKDLRKMCIFTIDPMTARDLDDAVSIEKLGDNEYEIGVHISDVSHFLIEDNELDNIVKERSTSIYLANEVIHMLPQSLCMRCSLLPGQDKFAFSVFWRMNGKGVMLQKKPEFCRTVINSCSQFAYEHAQKIIDNPNERFTENDFPTILNGFNPDDIRNRVLWLHDIASSIRKTRLDNGALTINNAKLRFLLDPITGEPLSFEVEKQREANRLIEEFMLLANQAVARFIHDSFPDIAVLRNHPPPLIKSLKALREKLLALGFELDYSSSKALQESMVRLCNEAPNPVAMNACLSQLLMKPMARATYFCSEGKSEPADLWHYALSIPIYTHFTSPIRRYPDILVHRLLAAALKYCTPPKRTPDDLHTLTKLANERKYNAKKAGEDSGNLYFKRYVHNKQGIYMRAVVIEIFQHMMNVVTLESGHVISINYKMQKVLVDTHGVPNYILIAERNLKQSPRKLQLLSVVPICLIIWDQKLTGFLKMEEQI

Target ORF information:

RefSeq Version	NM_138168.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138168.3

ORF Insert Sequence:

ATGTCGGAAA CGTCAAGCGT CAACGTGGAT GCGGACCGAG ATTGCAGAAG GATTGAGGGC 
CTCCGACAGC GCTCACAACG CCTTGCTGCC CGCCTCCAGC AGATGCAGAG GGAAATGGAG 
TCTAATGCAT CCAGCAAAGA CCTATCGGCC AACAATGCCG TTATTCCGCT GCAGCCACTA 
ATGCGCTGGG CGGAACCACG GCCACATCCC AACCAATCCC AACCATCTGC TCGGAGCAAC 
AGCAGGCAGG CCATTCCTTA CGGTGTGCCA GTATCCACGC CAGCGCCGGT AGCTATGCAA 
TTGCCCTACC ACGTAGCATT CGCCTCCTAC CAGGCGACCA TGCAGATTCC CTGCCTTCAG 
CCACTTTCGC ATTTGCACGT CGTGTCTAGT CCCAGCCATC GGTTAATAGC GAAAAAGAAG 
TCGAAGCGGG CTCTTCGAAA TGAGGTGGAA GCTCTTCAGG ATATGGTCAA ACATCTTTCA 
CACCTCTCCC CAGATGTTAA CGCGTATGCC TGCCAGCTGA TACGTAAAAA CGAGATCCTT 
TCTCAGCTCG AGTGGAGTCA GACCAGGTCA GAAGCACATC CGCAACAGGT TGGTTACCAG 
GGGCGGCATC GCCACGATTC TGAGGTCTCT GCTACAAACA ATGGAAGTCA TGTACAGCAG 
CAACCGAAAC TAGGACGAAG GCAACGCAAT AGCAAGAAAA ACGATGGATC CGGTAGCAAT 
GGGACCCAAT CGCAAAAAAC CGAACTAGAG GCCATGCGGT CGTACATAAA CAAGATTGTC 
CAACGTCACG TGGGTATGGA TCACCAGTTG TCGGAAGACT TGATTTTCAA AGGTAACTTC 
TCCGACCTGG AAGCGCATGC GCAGAGACTA GTTGCGGCGG GTTATGGGCG CATTGTAGAA 
GAGGAAATCC GGGTTAACCG CAAGAACAAC AGACAGGCCT TTATAATCAT GTCCACTGAT 
CGAGAAGCAC TCGAACGAGA TGGCATCGTG CTCTTGCCAG TGGCTAGGCG CTATGCCTTC 
GATGGTGACA AAGTACGTGC ATTCGTGCTA AACCCTGGTG CTCAGGGTAG TTCAAAGACA 
GCTGAGCCCT CGTCTGGTGA AATCTCTGGT GGGAAGCCCT CATTATCTCT TGCGGATGGT 
GAGGAGCTTT CGGACGATAC CGAGTCCCAG GGCTCTGAGT CCGATACCGA CAACGTGGTC 
GTGATATCGT CGGACAACTG CCCTAAAGCG TTCGTCATAG CAATAACTAA ACGAACTGAG 
CTCCGCCAGA TCGTGGGAAC CATATCGTTT ACGAACCCAA CCAAACTCTG TGACGACCAG 
CTATTCTACA AATTCCGACC GTACGATTTG CGTGTCCCCA TGGTCTACGT TCCGAAGGAT 
GCCTGTGCCG CCCACATCGG AAACAAGCAG CAAATAGACG TGTCCGGCCT CTTATACCTT 
GCCCATATAC TCGAGACAGA TTGCAATGGG CACTGCATCG CTGAACTAAT CCAGCCGGTT 
GGCCGCGTGG GTAATTTGGA TGACGAACTT AAGGCAATTC TGTTTCATAA CGGTCTCCGG 
GATATAAAGC CATTTGAGCA ACGATTTATC GACATTTACT CCCAGCCGCC ACCTCCGATA 
AGCCAAGAGG ATCTGCGTCA ACGAAAAGAC TTAAGAAAGA TGTGCATATT TACTATAGAT 
CCGATGACTG CTCGCGATTT GGATGACGCC GTTTCTATAG AGAAGCTAGG CGACAACGAA 
TATGAGATTG GCGTCCATAT ATCCGATGTT TCGCACTTCC TGATAGAGGA CAATGAACTG 
GATAACATAG TGAAGGAACG CTCTACGTCT ATTTATTTAG CCAACGAGGT AATCCACATG 
CTGCCTCAGT CTCTATGCAT GCGATGTTCC CTGCTCCCTG GGCAAGACAA GTTTGCTTTT 
TCCGTCTTCT GGCGGATGAA CGGGAAGGGA GTCATGCTGC AAAAGAAGCC AGAATTTTGC 
CGCACAGTCA TTAACTCTTG CTCGCAATTT GCTTATGAAC ACGCTCAAAA GATCATCGAC 
AACCCTAATG AACGGTTTAC CGAGAACGAC TTTCCGACCA TCCTGAATGG ATTTAATCCC 
GATGATATCA GAAATAGGGT GCTATGGCTG CACGACATTG CAAGCTCCAT CCGTAAGACA 
CGTTTAGACA ATGGCGCACT CACAATTAAC AATGCCAAGC TACGCTTCCT GCTCGATCCA 
ATTACTGGCG AGCCGTTGTC TTTTGAAGTG GAGAAACAGC GAGAGGCTAA CCGGCTGATT 
GAAGAGTTTA TGCTCCTGGC CAACCAGGCA GTCGCCCGCT TTATACACGA CTCCTTTCCG 
GATATCGCTG TACTGCGTAA CCACCCTCCG CCACTTATTA AGTCCCTTAA GGCTTTAAGG 
GAAAAGCTGC TTGCTCTGGG ATTCGAATTG GACTATAGCT CGTCTAAGGC GCTCCAGGAG 
AGCATGGTGC GGTTGTGCAA TGAGGCGCCC AATCCTGTCG CAATGAATGC CTGCCTAAGT 
CAGCTTTTGA TGAAACCAAT GGCCCGTGCA ACATATTTTT GTAGCGAGGG TAAATCGGAA 
CCGGCTGACC TGTGGCACTA CGCCTTGTCC ATACCCATAT ACACTCACTT TACTAGCCCA 
ATCCGTCGGT ATCCTGATAT TTTGGTGCAC CGACTTTTGG CGGCCGCACT TAAATATTGT 
ACACCGCCTA AACGTACCCC AGATGATTTG CACACCTTAA CGAAATTAGC GAATGAGCGC 
AAGTACAATG CGAAAAAGGC CGGAGAGGAC TCTGGTAACT TGTACTTCAA ACGATATGTT 
CACAACAAGC AGGGCATATA CATGCGAGCT GTGGTCATTG AAATATTTCA GCATATGATG 
AACGTAGTTA CTCTGGAGTC GGGCCACGTT ATAAGCATTA ACTACAAAAT GCAAAAGGTC 
CTCGTAGACA CACACGGCGT ACCCAATTAC ATTCTTATTG CCGAGCGCAA CTTAAAGCAG 
TCACCTAGGA AGTTGCAGCT GCTTTCCGTG GTCCCCATAT GTCTGATCAT TTGGGACCAA 
AAACTAACTG GATTTTTAAA GATGGAAGAA CAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dis3l2 ( NM_167805.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728490.1 Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17045
Clone ID Related Accession (Same CDS sequence)	NM_167805.2
Accession Version	NM_167805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dis3 like 3'-5' exoribonuclease 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_167805.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTTACC CTTTATATCC CGCCCATAGC CAAGTAATGT CGGAAACGTC AAGCGTCAAC 
GTGGATGCGG ACCGAGATTG CAGAAGGATT GAGGGCCTCC GACAGCGCTC ACAACGCCTT 
GCTGCCCGCC TCCAGCAGAT GCAGAGGGAA ATGGAGTCTA ATGCATCCAG CAAAGACCTA 
TCGGCCAACA ATGCCGTTAT TCCGCTGCAG CCACTAATGC GCTGGGCGGA ACCACGGCCA 
CATCCCAACC AATCCCAACC ATCTGCTCGG AGCAACAGCA GGCAGGCCAT TCCTTACGGT 
GTGCCAGTAT CCACGCCAGC GCCGGTAGCT ATGCAATTGC CCTACCACGT AGCATTCGCC 
TCCTACCAGG CGACCATGCA GATTCCCTGC CTTCAGCCAC TTTCGCATTT GCACGTCGTG 
TCTAGTCCCA GCCATCGGTT AATAGCGAAA AAGAAGTCGA AGCGGGCTCT TCGAAATGAG 
GTGGAAGCTC TTCAGGATAT GGTCAAACAT CTTTCACACC TCTCCCCAGA TGTTAACGCG 
TATGCCTGCC AGCTGATACG TAAAAACGAG ATCCTTTCTC AGCTCGAGTG GAGTCAGACC 
AGGTCAGAAG CACATCCGCA ACAGGTTGGT TACCAGGGGC GGCATCGCCA CGATTCTGAG 
GTCTCTGCTA CAAACAATGG AAGTCATGTA CAGCAGCAAC CGAAACTAGG ACGAAGGCAA 
CGCAATAGCA AGAAAAACGA TGGATCCGGT AGCAATGGGA CCCAATCGCA AAAAACCGAA 
CTAGAGGCCA TGCGGTCGTA CATAAACAAG ATTGTCCAAC GTCACGTGGG TATGGATCAC 
CAGTTGTCGG AAGACTTGAT TTTCAAAGGT AACTTCTCCG ACCTGGAAGC GCATGCGCAG 
AGACTAGTTG CGGCGGGTTA TGGGCGCATT GTAGAAGAGG AAATCCGGGT TAACCGCAAG 
AACAACAGAC AGGCCTTTAT AATCATGTCC ACTGATCGAG AAGCACTCGA ACGAGATGGC 
ATCGTGCTCT TGCCAGTGGC TAGGCGCTAT GCCTTCGATG GTGACAAAGT ACGTGCATTC 
GTGCTAAACC CTGGTGCTCA GGGTAGTTCA AAGACAGCTG AGCCCTCGTC TGGTGAAATC 
TCTGGTGGGA AGCCCTCATT ATCTCTTGCG GATGGTGAGG AGCTTTCGGA CGATACCGAG 
TCCCAGGGCT CTGAGTCCGA TACCGACAAC GTGGTCGTGA TATCGTCGGA CAACTGCCCT 
AAAGCGTTCG TCATAGCAAT AACTAAACGA ACTGAGCTCC GCCAGATCGT GGGAACCATA 
TCGTTTACGA ACCCAACCAA ACTCTGTGAC GACCAGCTAT TCTACAAATT CCGACCGTAC 
GATTTGCGTG TCCCCATGGT CTACGTTCCG AAGGATGCCT GTGCCGCCCA CATCGGAAAC 
AAGCAGCAAA TAGACGTGTC CGGCCTCTTA TACCTTGCCC ATATACTCGA GACAGATTGC 
AATGGGCACT GCATCGCTGA ACTAATCCAG CCGGTTGGCC GCGTGGGTAA TTTGGATGAC 
GAACTTAAGG CAATTCTGTT TCATAACGGT CTCCGGGATA TAAAGCCATT TGAGCAACGA 
TTTATCGACA TTTACTCCCA GCCGCCACCT CCGATAAGCC AAGAGGATCT GCGTCAACGA 
AAAGACTTAA GAAAGATGTG CATATTTACT ATAGATCCGA TGACTGCTCG CGATTTGGAT 
GACGCCGTTT CTATAGAGAA GCTAGGCGAC AACGAATATG AGATTGGCGT CCATATATCC 
GATGTTTCGC ACTTCCTGAT AGAGGACAAT GAACTGGATA ACATAGTGAA GGAACGCTCT 
ACGTCTATTT ATTTAGCCAA CGAGGTAATC CACATGCTGC CTCAGTCTCT ATGCATGCGA 
TGTTCCCTGC TCCCTGGGCA AGACAAGTTT GCTTTTTCCG TCTTCTGGCG GATGAACGGG 
AAGGGAGTCA TGCTGCAAAA GAAGCCAGAA TTTTGCCGCA CAGTCATTAA CTCTTGCTCG 
CAATTTGCTT ATGAACACGC TCAAAAGATC ATCGACAACC CTAATGAACG GTTTACCGAG 
AACGACTTTC CGACCATCCT GAATGGATTT AATCCCGATG ATATCAGAAA TAGGGTGCTA 
TGGCTGCACG ACATTGCAAG CTCCATCCGT AAGACACGTT TAGACAATGG CGCACTCACA 
ATTAACAATG CCAAGCTACG CTTCCTGCTC GATCCAATTA CTGGCGAGCC GTTGTCTTTT 
GAAGTGGAGA AACAGCGAGA GGCTAACCGG CTGATTGAAG AGTTTATGCT CCTGGCCAAC 
CAGGCAGTCG CCCGCTTTAT ACACGACTCC TTTCCGGATA TCGCTGTACT GCGTAACCAC 
CCTCCGCCAC TTATTAAGTC CCTTAAGGCT TTAAGGGAAA AGCTGCTTGC TCTGGGATTC 
GAATTGGACT ATAGCTCGTC TAAGGCGCTC CAGGAGAGCA TGGTGCGGTT GTGCAATGAG 
GCGCCCAATC CTGTCGCAAT GAATGCCTGC CTAAGTCAGC TTTTGATGAA ACCAATGGCC 
CGTGCAACAT ATTTTTGTAG CGAGGGTAAA TCGGAACCGG CTGACCTGTG GCACTACGCC 
TTGTCCATAC CCATATACAC TCACTTTACT AGCCCAATCC GTCGGTATCC TGATATTTTG 
GTGCACCGAC TTTTGGCGGC CGCACTTAAA TATTGTACAC CGCCTAAACG TACCCCAGAT 
GATTTGCACA CCTTAACGAA ATTAGCGAAT GAGCGCAAGT ACAATGCGAA AAAGGCCGGA 
GAGGACTCTG GTAACTTGTA CTTCAAACGA TATGTTCACA ACAAGCAGGG CATATACATG 
CGAGCTGTGG TCATTGAAAT ATTTCAGCAT ATGATGAACG TAGTTACTCT GGAGTCGGGC 
CACGTTATAA GCATTAACTA CAAAATGCAA AAGGTCCTCG TAGACACACA CGGCGTACCC 
AATTACATTC TTATTGCCGA GCGCAACTTA AAGCAGTCAC CTAGGAAGTT GCAGCTGCTT 
TCCGTGGTCC CCATATGTCT GATCATTTGG GACCAAAAAC TAACTGGATT TTTAAAGATG 
GAAGAACAAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728490.1
CDS	42..3176
Translation	MPYPLYPAHSQVMSETSSVNVDADRDCRRIEGLRQRSQRLAARLQQMQREMESNASSKDLSANNAVIPLQPLMRWAEPRPHPNQSQPSARSNSRQAIPYGVPVSTPAPVAMQLPYHVAFASYQATMQIPCLQPLSHLHVVSSPSHRLIAKKKSKRALRNEVEALQDMVKHLSHLSPDVNAYACQLIRKNEILSQLEWSQTRSEAHPQQVGYQGRHRHDSEVSATNNGSHVQQQPKLGRRQRNSKKNDGSGSNGTQSQKTELEAMRSYINKIVQRHVGMDHQLSEDLIFKGNFSDLEAHAQRLVAAGYGRIVEEEIRVNRKNNRQAFIIMSTDREALERDGIVLLPVARRYAFDGDKVRAFVLNPGAQGSSKTAEPSSGEISGGKPSLSLADGEELSDDTESQGSESDTDNVVVISSDNCPKAFVIAITKRTELRQIVGTISFTNPTKLCDDQLFYKFRPYDLRVPMVYVPKDACAAHIGNKQQIDVSGLLYLAHILETDCNGHCIAELIQPVGRVGNLDDELKAILFHNGLRDIKPFEQRFIDIYSQPPPPISQEDLRQRKDLRKMCIFTIDPMTARDLDDAVSIEKLGDNEYEIGVHISDVSHFLIEDNELDNIVKERSTSIYLANEVIHMLPQSLCMRCSLLPGQDKFAFSVFWRMNGKGVMLQKKPEFCRTVINSCSQFAYEHAQKIIDNPNERFTENDFPTILNGFNPDDIRNRVLWLHDIASSIRKTRLDNGALTINNAKLRFLLDPITGEPLSFEVEKQREANRLIEEFMLLANQAVARFIHDSFPDIAVLRNHPPPLIKSLKALREKLLALGFELDYSSSKALQESMVRLCNEAPNPVAMNACLSQLLMKPMARATYFCSEGKSEPADLWHYALSIPIYTHFTSPIRRYPDILVHRLLAAALKYCTPPKRTPDDLHTLTKLANERKYNAKKAGEDSGNLYFKRYVHNKQGIYMRAVVIEIFQHMMNVVTLESGHVISINYKMQKVLVDTHGVPNYILIAERNLKQSPRKLQLLSVVPICLIIWDQKLTGFLKMEEQI

Target ORF information:

RefSeq Version	NM_167805.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dis3 like 3'-5' exoribonuclease 2 (Dis3l2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167805.2

ORF Insert Sequence:

ATGCCTTACC CTTTATATCC CGCCCATAGC CAAGTAATGT CGGAAACGTC AAGCGTCAAC 
GTGGATGCGG ACCGAGATTG CAGAAGGATT GAGGGCCTCC GACAGCGCTC ACAACGCCTT 
GCTGCCCGCC TCCAGCAGAT GCAGAGGGAA ATGGAGTCTA ATGCATCCAG CAAAGACCTA 
TCGGCCAACA ATGCCGTTAT TCCGCTGCAG CCACTAATGC GCTGGGCGGA ACCACGGCCA 
CATCCCAACC AATCCCAACC ATCTGCTCGG AGCAACAGCA GGCAGGCCAT TCCTTACGGT 
GTGCCAGTAT CCACGCCAGC GCCGGTAGCT ATGCAATTGC CCTACCACGT AGCATTCGCC 
TCCTACCAGG CGACCATGCA GATTCCCTGC CTTCAGCCAC TTTCGCATTT GCACGTCGTG 
TCTAGTCCCA GCCATCGGTT AATAGCGAAA AAGAAGTCGA AGCGGGCTCT TCGAAATGAG 
GTGGAAGCTC TTCAGGATAT GGTCAAACAT CTTTCACACC TCTCCCCAGA TGTTAACGCG 
TATGCCTGCC AGCTGATACG TAAAAACGAG ATCCTTTCTC AGCTCGAGTG GAGTCAGACC 
AGGTCAGAAG CACATCCGCA ACAGGTTGGT TACCAGGGGC GGCATCGCCA CGATTCTGAG 
GTCTCTGCTA CAAACAATGG AAGTCATGTA CAGCAGCAAC CGAAACTAGG ACGAAGGCAA 
CGCAATAGCA AGAAAAACGA TGGATCCGGT AGCAATGGGA CCCAATCGCA AAAAACCGAA 
CTAGAGGCCA TGCGGTCGTA CATAAACAAG ATTGTCCAAC GTCACGTGGG TATGGATCAC 
CAGTTGTCGG AAGACTTGAT TTTCAAAGGT AACTTCTCCG ACCTGGAAGC GCATGCGCAG 
AGACTAGTTG CGGCGGGTTA TGGGCGCATT GTAGAAGAGG AAATCCGGGT TAACCGCAAG 
AACAACAGAC AGGCCTTTAT AATCATGTCC ACTGATCGAG AAGCACTCGA ACGAGATGGC 
ATCGTGCTCT TGCCAGTGGC TAGGCGCTAT GCCTTCGATG GTGACAAAGT ACGTGCATTC 
GTGCTAAACC CTGGTGCTCA GGGTAGTTCA AAGACAGCTG AGCCCTCGTC TGGTGAAATC 
TCTGGTGGGA AGCCCTCATT ATCTCTTGCG GATGGTGAGG AGCTTTCGGA CGATACCGAG 
TCCCAGGGCT CTGAGTCCGA TACCGACAAC GTGGTCGTGA TATCGTCGGA CAACTGCCCT 
AAAGCGTTCG TCATAGCAAT AACTAAACGA ACTGAGCTCC GCCAGATCGT GGGAACCATA 
TCGTTTACGA ACCCAACCAA ACTCTGTGAC GACCAGCTAT TCTACAAATT CCGACCGTAC 
GATTTGCGTG TCCCCATGGT CTACGTTCCG AAGGATGCCT GTGCCGCCCA CATCGGAAAC 
AAGCAGCAAA TAGACGTGTC CGGCCTCTTA TACCTTGCCC ATATACTCGA GACAGATTGC 
AATGGGCACT GCATCGCTGA ACTAATCCAG CCGGTTGGCC GCGTGGGTAA TTTGGATGAC 
GAACTTAAGG CAATTCTGTT TCATAACGGT CTCCGGGATA TAAAGCCATT TGAGCAACGA 
TTTATCGACA TTTACTCCCA GCCGCCACCT CCGATAAGCC AAGAGGATCT GCGTCAACGA 
AAAGACTTAA GAAAGATGTG CATATTTACT ATAGATCCGA TGACTGCTCG CGATTTGGAT 
GACGCCGTTT CTATAGAGAA GCTAGGCGAC AACGAATATG AGATTGGCGT CCATATATCC 
GATGTTTCGC ACTTCCTGAT AGAGGACAAT GAACTGGATA ACATAGTGAA GGAACGCTCT 
ACGTCTATTT ATTTAGCCAA CGAGGTAATC CACATGCTGC CTCAGTCTCT ATGCATGCGA 
TGTTCCCTGC TCCCTGGGCA AGACAAGTTT GCTTTTTCCG TCTTCTGGCG GATGAACGGG 
AAGGGAGTCA TGCTGCAAAA GAAGCCAGAA TTTTGCCGCA CAGTCATTAA CTCTTGCTCG 
CAATTTGCTT ATGAACACGC TCAAAAGATC ATCGACAACC CTAATGAACG GTTTACCGAG 
AACGACTTTC CGACCATCCT GAATGGATTT AATCCCGATG ATATCAGAAA TAGGGTGCTA 
TGGCTGCACG ACATTGCAAG CTCCATCCGT AAGACACGTT TAGACAATGG CGCACTCACA 
ATTAACAATG CCAAGCTACG CTTCCTGCTC GATCCAATTA CTGGCGAGCC GTTGTCTTTT 
GAAGTGGAGA AACAGCGAGA GGCTAACCGG CTGATTGAAG AGTTTATGCT CCTGGCCAAC 
CAGGCAGTCG CCCGCTTTAT ACACGACTCC TTTCCGGATA TCGCTGTACT GCGTAACCAC 
CCTCCGCCAC TTATTAAGTC CCTTAAGGCT TTAAGGGAAA AGCTGCTTGC TCTGGGATTC 
GAATTGGACT ATAGCTCGTC TAAGGCGCTC CAGGAGAGCA TGGTGCGGTT GTGCAATGAG 
GCGCCCAATC CTGTCGCAAT GAATGCCTGC CTAAGTCAGC TTTTGATGAA ACCAATGGCC 
CGTGCAACAT ATTTTTGTAG CGAGGGTAAA TCGGAACCGG CTGACCTGTG GCACTACGCC 
TTGTCCATAC CCATATACAC TCACTTTACT AGCCCAATCC GTCGGTATCC TGATATTTTG 
GTGCACCGAC TTTTGGCGGC CGCACTTAAA TATTGTACAC CGCCTAAACG TACCCCAGAT 
GATTTGCACA CCTTAACGAA ATTAGCGAAT GAGCGCAAGT ACAATGCGAA AAAGGCCGGA 
GAGGACTCTG GTAACTTGTA CTTCAAACGA TATGTTCACA ACAAGCAGGG CATATACATG 
CGAGCTGTGG TCATTGAAAT ATTTCAGCAT ATGATGAACG TAGTTACTCT GGAGTCGGGC 
CACGTTATAA GCATTAACTA CAAAATGCAA AAGGTCCTCG TAGACACACA CGGCGTACCC 
AATTACATTC TTATTGCCGA GCGCAACTTA AAGCAGTCAC CTAGGAAGTT GCAGCTGCTT 
TCCGTGGTCC CCATATGTCT GATCATTTGG GACCAAAAAC TAACTGGATT TTTAAAGATG 
GAAGAACAAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dis3l2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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