egg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	egg
Entrez Gene ID	37962
Full Name	eggless
Synonyms	AAF47268 Dm,AAM70794,BcDNA:AT13877,CG12196,CG30422,CG30426,Dm-setdb1,DmSetdb1,Dmel\CG12196,Dmel_CG30422,Dmel_CG30426,Dmsetdb1,EGG,Egg,Egg/dSETDB1,Eggless,SETDB1,SetDB1,Setdb1,dEset,dSETDB1,dSetdb1,dsetdb1
General protein information	Preferred Names eggless Names CG12196 gene product from transcript CG12196-RA CG12196-PA Histone-lysine N-methyltransferase dSETDB1 egg-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60E1-60E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_138122.5	NP_611966.3	eggless

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	egg	NP_611966.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007978	XP_317488.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified a H3K9 methyltransferase, SETDB1/eggless, a Drosophila homologue of the mammalian SETDB1, which confers the robustness for the precise determination of bristle number.
Title: Drosophila SETDB1 and caspase cooperatively fine-tune cell fate determination of sensory organ precursor.

dSETDB1 is required for the expression of a Wnt ligand, Drosophila Wingless type mouse mammary virus integration site number 4 (dWnt4) in the somatic niche.
Title: Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila.

Mosaic analysis also reveals that the early egg chamber budding defects in egg- ovaries are due to an intrinsic requirement for egg in follicle stem cells and their descendents
Title: Epigenetic regulation of oogenesis and germ stem cell maintenance by the Drosophila histone methyltransferase Eggless/dSetDB1.

The results showed that on chromosome 4, Su(var)3-9, SETDB1 and HP1a target the same genes.
Title: HP1a, Su(var)3-9, SETDB1 and POF stimulate or repress gene expression depending on genomic position, gene length and expression pattern in Drosophila melanogaster.

this study has revealed the essential roles of Eggless (Egg), a H3K9 methyltransferase in controlling stem cell maintenance and differentiation through distinct mechanisms.
Title: Histone H3K9 trimethylase Eggless controls germline stem cell maintenance and differentiation.

The following egg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11235	NM_138122.5 Latest version!	Drosophila melanogaster eggless (egg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egg ( NM_138122.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611966.3 Drosophila melanogaster eggless (egg), mRNA.

Price & Availability↑

CloneID	OFa11235
Clone ID Related Accession (Same CDS sequence)	NM_138122.5
Accession Version	NM_138122.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eggless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_138122.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGGC AGCCAACAGC CGTGGACTGC TTAGAGAGCA GTGGAAGCAC AGTGGAGGAT 
GTCCAAGAAA CACCGGCGTC GAGGGAAAAA AGTTACGGGT TGCCGGTGCG CAAAGGAGAA 
AATAGCTTGG AGTCTCCTGC AGAACAAGCC GCAAAGGATG TGGAAATTGA GGAACTGACA 
CACAGTGAAG CAATCGCCGC CACCGGTTCA ACCAGGAAGC AGTGCCCATA TGGAGGTAAG 
GCCCCTGATG AACCTGGAAA GTTGGCCGAC GAATCGGAGG ACCGCAAGGG AGAGAACACG 
AAAGCCATTG CAAGCAGCCC GGTTTTGGTA GCTGTGGATT CGGACAGCAG TGTTGAGCTA 
ATAGAGAGTC CTGTCAAATT CTCCTCCGCC AACGAAAGTG AGAAAGATCC GCCAAAACCA 
GATGCTGTCA ATGAAGCTGC GGCTAAAGAG GCCGAGGAAA TGACTGATAG CAGCATCAGC 
AGTCCCACAT CCGAAAGTTT TCCTGAAAAA GATGAAAAGA CCAATAAGGA AAATGAGCAA 
GAACCTCCTG GCATGGAGGT AGATCAGGAC GTCGAGGAGT CAATAAGTCG ACCAGCAGAA 
GAATACAAAA TTGAGAATAC ATTAAAAGGT CACAAGCGTA TTAGCTTAAC GGAAATCGAA 
GAACACAAAA TTGTTGATAA AAAAGACGAT GTCCTAGAGG TGGAGCTTGA AAAGGGCACT 
GCTCCGAAAG CTGCAGAAGA CGAGAAGCTG AACGCTTTGT TATCAGATGG AGATGTCTTC 
TACGACAAGG AATGTGTCAA CTGCAATTGC ACAAAGCTTC ATAAACAGTA CGTGTTGGCT 
AACATGGCCA CTTTAAACTT TTACCAGGTG CTCCGAAAGT CTTCCAAGCA GCAGTTCCTC 
TGCATGGGAT GCCATGACAC AGCAATGGAC TTGTATGAGG AGTATGCTGG ACAACTAATG 
GCCAAGCAGC CGCTGCTGCT AAAGGATTTC CATCAGGATC ACGCCGACTT TGTGGCTCTG 
GACAGCAGCG ATGAGGAGGA AGAGGAGAAG CAGCCAGAAA AATCGGACTT TTCGAAAAAC 
AAGCTGCAGC TCATCGAAGA TGAACTCGAT GATGCCATCA AAAATGTACT TAACAAGGTG 
GATTTTACGG CTCAGCTGTC CTGGTCAAAA ACTATTCTCC AGGCGAAAGC AGACCACTTG 
GAGCGACAAT TCGCACTGGC AGATGTGGAA CTCGAAAAAG TTCAGACCAC CGCTGACAAA 
ATGCACTGCG CTTTGTATAA CTCGTGTCCG GTGGCACACA AACATCTGCC CACCCTGGAC 
ATTGAACCCA GTGACTATGT GCATGAAGTT CCACCTCCTG GCGAAATCGT GCGTCCTCCA 
ATACAGTTGG GAGAGACATA CTACGCTGTT AAGAACAAGG CTATTGCCAG CTGGGTGTCC 
ATAAAGGTAA TCGAGTTTAC AGAGAGCACC GCCATCAACG GAAACACGAT GAAGAGCTAC 
AAGATTAGGT ACCTCAACAC TCCGTACCAG ATGATCAAGA CAGTGACGGC CAAGCACATT 
GCCTACTTCG AACCACCGCC AGTGCGACTG ACCATTGGTA CCCGCGTGAT TGCGTACTTC 
GATGGCACCA CTTTGTCGCG GGGCAAGGAT AAGGGCGTGG TGCAGAGCGC CTTTTATCCA 
GGCATCATCG CTGAGCCGTT GAAGCAGGCC AATCGCTACC GCTACCTAAT ATTCTACGAC 
GATGGGTACA CTCAGTACGT TCCGCACCGC GATGTCCGTC TAGTTTGCCA GGCTTCCGAG 
AAAGTGTGGG AGGACGTACA CGCCGCATCG CGCGATTTCA TACAGAAGTA CGTGGAGAAG 
TACTCCGTGG ATCGTCCAAT GGTGCAATGC ACCCGGGGAC AAAGTATGAC CACTGAATCG 
AACGGGACCT GGCTGTACGC GCGCGTCATT GACATCGACT GCAGCTTGGT GTTGATGCAG 
TTTGAGGGCG ACAAAAACCA TACGGAGTGG ATATACAGGG GTTCATTGCG CTTGGGTCCT 
GTCTTCAGGG AAACCCAGAA CAATATGAAC AGTTCAAGCG CACAGCAGCT GCGTGTCCCA 
AGACGCACGG AGCCCTTTAT TCGGTACACC AAGGAGATGG AGTCCAGCAG CAAGGTGAAC 
CAGCAGATGC GTGCCTTTGC CCGCAAGAGC AGCGCCTCCG CCCAAAATAA CGCATTAGCG 
GCTGCATCTT CGGCAGCAAC CCCAGCCGGA GGACGCACCA ATGCAGGAGG TGTCAGCACC 
AGTAATAGCG CAAGTGCTGT GCGACACCTA AACAACTCGA CAATCTACGT GGACGACGAG 
AACAGGCCCA AGGGCCATGT AGTCTACTTT ACAGCCAAGC GGAATCTGCC TCCCAAAATG 
TACAAGTGCC ACGAGTGCAG CCCGAACTGC CTCTTTAAGA TCGTTCACCG TCTGGACAGC 
TACAGTCCGT TAGCGAAGCC TCTGCTTTCT GGCTGGGAAC GTCTTGTCAT GCGGCAGAAG 
ACCAAGAAGT CTGTGGTATA CAAGGGACCT TGTGGCAAAA GCTTGCGCAG CCTGGCCGAA 
GTTCACAGGT ACCTTCGAGC TACTGAAAAT GTGTTGAACG TAGACAATTT TGACTTTACG 
CCCGACCTCA AGTGTCTGGC CGAGTACTCC ATTGATCCCT CGATCGTGAA GGACACGGAC 
ATTTCAAAGG GGCAGGAGAA GATGGCGATT CCGCTGGTAA ACTACTACGA CAACACCCTG 
CCGCCCCCAT GCACCTACGC GAAGCAGCGC ATCCCTACCG AGGGTGTTCA TCTGAATCTG 
GACGAGGAGT TCCTCTTGTG CTGCGACTGC GAGGACGACT GCTCGGACAA GTCAAAGTGC 
GCCTGTTGGC AGCTAACGGT GGCGGGCGTG CGGTACTGTA ACCCGAAGAA ACCCATCGAG 
GAGATAGGCT ACCAGTACAA GCGGCTCCAC GAGCACGTGC CCACCGGCAT CTACGAGTGC 
AACTCGCGCT GCAAGTGCAA GAAGAATTGC CTGAACCGGG TGGTGCAGTT CTCCCTAGAG 
ATGAAACTGC AGGTGTTCAA GACCTCAAAT CGTGGATGGG GCTTGCGCTG CGTTAACGAC 
ATCCCCAAGG GTGCTTTCAT CTGTATTTAC GCCGGCCATC TGCTCACGGA GACGATGGCC 
AACGAAGGCG GACAGGACGC CGGCGACGAG TATTTCGCGG ACTTAGATTA CATAGAGGTC 
GCTGAACAGT TAAAAGAGGG ATACGAGTCG GAGGTGGACC ACTCGGACCC GGACGCCGAG 
GAGGACAACG GGGGACCCGA TGCCGAGGAC GATGACGACT TCCGGCCAAA CTATCACTAC 
CAGCGCAAAA TCAAGCGCTC TTCCAGAAGC GGCTCCACGC AGAACAGCTC CACTCAGAGC 
AGCGAGTTGG ACTCGCAGGA GCGTGCGGTG ATCAACTTTA ACCCAAATGC TGATCTGGAC 
GAGACGGTGC GGGAGAACTC GGTGCGGAGG CTTTTCGGCA AGGACGAGGC GCCCTACATC 
ATGGACGCCA AGACAACGGG CAACCTTGGA CGCTATTTCA ACCACTCGTG CAGCCCCAAT 
CTCTTCGTGC AGAACGTGTT CGTGGATACT CACGACCTGC GTTTCCCCTG GGTTGCCTTC 
TTCTCGGCCG CGCACATCCG CTCCGGCACC GAGCTGACTT GGAACTACAA TTACGAGGTG 
GGCGTGGTGC CCGGCAAGGT GTTGTACTGC CAGTGCGGGG CCCCCAATTG CCGCCTTCGT 
CTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611966.3
CDS	89..3877
Translation	MSGQPTAVDCLESSGSTVEDVQETPASREKSYGLPVRKGENSLESPAEQAAKDVEIEELTHSEAIAATGSTRKQCPYGGKAPDEPGKLADESEDRKGENTKAIASSPVLVAVDSDSSVELIESPVKFSSANESEKDPPKPDAVNEAAAKEAEEMTDSSISSPTSESFPEKDEKTNKENEQEPPGMEVDQDVEESISRPAEEYKIENTLKGHKRISLTEIEEHKIVDKKDDVLEVELEKGTAPKAAEDEKLNALLSDGDVFYDKECVNCNCTKLHKQYVLANMATLNFYQVLRKSSKQQFLCMGCHDTAMDLYEEYAGQLMAKQPLLLKDFHQDHADFVALDSSDEEEEEKQPEKSDFSKNKLQLIEDELDDAIKNVLNKVDFTAQLSWSKTILQAKADHLERQFALADVELEKVQTTADKMHCALYNSCPVAHKHLPTLDIEPSDYVHEVPPPGEIVRPPIQLGETYYAVKNKAIASWVSIKVIEFTESTAINGNTMKSYKIRYLNTPYQMIKTVTAKHIAYFEPPPVRLTIGTRVIAYFDGTTLSRGKDKGVVQSAFYPGIIAEPLKQANRYRYLIFYDDGYTQYVPHRDVRLVCQASEKVWEDVHAASRDFIQKYVEKYSVDRPMVQCTRGQSMTTESNGTWLYARVIDIDCSLVLMQFEGDKNHTEWIYRGSLRLGPVFRETQNNMNSSSAQQLRVPRRTEPFIRYTKEMESSSKVNQQMRAFARKSSASAQNNALAAASSAATPAGGRTNAGGVSTSNSASAVRHLNNSTIYVDDENRPKGHVVYFTAKRNLPPKMYKCHECSPNCLFKIVHRLDSYSPLAKPLLSGWERLVMRQKTKKSVVYKGPCGKSLRSLAEVHRYLRATENVLNVDNFDFTPDLKCLAEYSIDPSIVKDTDISKGQEKMAIPLVNYYDNTLPPPCTYAKQRIPTEGVHLNLDEEFLLCCDCEDDCSDKSKCACWQLTVAGVRYCNPKKPIEEIGYQYKRLHEHVPTGIYECNSRCKCKKNCLNRVVQFSLEMKLQVFKTSNRGWGLRCVNDIPKGAFICIYAGHLLTETMANEGGQDAGDEYFADLDYIEVAEQLKEGYESEVDHSDPDAEEDNGGPDAEDDDDFRPNYHYQRKIKRSSRSGSTQNSSTQSSELDSQERAVINFNPNADLDETVRENSVRRLFGKDEAPYIMDAKTTGNLGRYFNHSCSPNLFVQNVFVDTHDLRFPWVAFFSAAHIRSGTELTWNYNYEVGVVPGKVLYCQCGAPNCRLRLL

Target ORF information:

RefSeq Version	NM_138122.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eggless (egg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138122.5

ORF Insert Sequence:

ATGTCTGGGC AGCCAACAGC CGTGGACTGC TTAGAGAGCA GTGGAAGCAC AGTGGAGGAT 
GTCCAAGAAA CACCGGCGTC GAGGGAAAAA AGTTACGGGT TGCCGGTGCG CAAAGGAGAA 
AATAGCTTGG AGTCTCCTGC AGAACAAGCC GCAAAGGATG TGGAAATTGA GGAACTGACA 
CACAGTGAAG CAATCGCCGC CACCGGTTCA ACCAGGAAGC AGTGCCCATA TGGAGGTAAG 
GCCCCTGATG AACCTGGAAA GTTGGCCGAC GAATCGGAGG ACCGCAAGGG AGAGAACACG 
AAAGCCATTG CAAGCAGCCC GGTTTTGGTA GCTGTGGATT CGGACAGCAG TGTTGAGCTA 
ATAGAGAGTC CTGTCAAATT CTCCTCCGCC AACGAAAGTG AGAAAGATCC GCCAAAACCA 
GATGCTGTCA ATGAAGCTGC GGCTAAAGAG GCCGAGGAAA TGACTGATAG CAGCATCAGC 
AGTCCCACAT CCGAAAGTTT TCCTGAAAAA GATGAAAAGA CCAATAAGGA AAATGAGCAA 
GAACCTCCTG GCATGGAGGT AGATCAGGAC GTCGAGGAGT CAATAAGTCG ACCAGCAGAA 
GAATACAAAA TTGAGAATAC ATTAAAAGGT CACAAGCGTA TTAGCTTAAC GGAAATCGAA 
GAACACAAAA TTGTTGATAA AAAAGACGAT GTCCTAGAGG TGGAGCTTGA AAAGGGCACT 
GCTCCGAAAG CTGCAGAAGA CGAGAAGCTG AACGCTTTGT TATCAGATGG AGATGTCTTC 
TACGACAAGG AATGTGTCAA CTGCAATTGC ACAAAGCTTC ATAAACAGTA CGTGTTGGCT 
AACATGGCCA CTTTAAACTT TTACCAGGTG CTCCGAAAGT CTTCCAAGCA GCAGTTCCTC 
TGCATGGGAT GCCATGACAC AGCAATGGAC TTGTATGAGG AGTATGCTGG ACAACTAATG 
GCCAAGCAGC CGCTGCTGCT AAAGGATTTC CATCAGGATC ACGCCGACTT TGTGGCTCTG 
GACAGCAGCG ATGAGGAGGA AGAGGAGAAG CAGCCAGAAA AATCGGACTT TTCGAAAAAC 
AAGCTGCAGC TCATCGAAGA TGAACTCGAT GATGCCATCA AAAATGTACT TAACAAGGTG 
GATTTTACGG CTCAGCTGTC CTGGTCAAAA ACTATTCTCC AGGCGAAAGC AGACCACTTG 
GAGCGACAAT TCGCACTGGC AGATGTGGAA CTCGAAAAAG TTCAGACCAC CGCTGACAAA 
ATGCACTGCG CTTTGTATAA CTCGTGTCCG GTGGCACACA AACATCTGCC CACCCTGGAC 
ATTGAACCCA GTGACTATGT GCATGAAGTT CCACCTCCTG GCGAAATCGT GCGTCCTCCA 
ATACAGTTGG GAGAGACATA CTACGCTGTT AAGAACAAGG CTATTGCCAG CTGGGTGTCC 
ATAAAGGTAA TCGAGTTTAC AGAGAGCACC GCCATCAACG GAAACACGAT GAAGAGCTAC 
AAGATTAGGT ACCTCAACAC TCCGTACCAG ATGATCAAGA CAGTGACGGC CAAGCACATT 
GCCTACTTCG AACCACCGCC AGTGCGACTG ACCATTGGTA CCCGCGTGAT TGCGTACTTC 
GATGGCACCA CTTTGTCGCG GGGCAAGGAT AAGGGCGTGG TGCAGAGCGC CTTTTATCCA 
GGCATCATCG CTGAGCCGTT GAAGCAGGCC AATCGCTACC GCTACCTAAT ATTCTACGAC 
GATGGGTACA CTCAGTACGT TCCGCACCGC GATGTCCGTC TAGTTTGCCA GGCTTCCGAG 
AAAGTGTGGG AGGACGTACA CGCCGCATCG CGCGATTTCA TACAGAAGTA CGTGGAGAAG 
TACTCCGTGG ATCGTCCAAT GGTGCAATGC ACCCGGGGAC AAAGTATGAC CACTGAATCG 
AACGGGACCT GGCTGTACGC GCGCGTCATT GACATCGACT GCAGCTTGGT GTTGATGCAG 
TTTGAGGGCG ACAAAAACCA TACGGAGTGG ATATACAGGG GTTCATTGCG CTTGGGTCCT 
GTCTTCAGGG AAACCCAGAA CAATATGAAC AGTTCAAGCG CACAGCAGCT GCGTGTCCCA 
AGACGCACGG AGCCCTTTAT TCGGTACACC AAGGAGATGG AGTCCAGCAG CAAGGTGAAC 
CAGCAGATGC GTGCCTTTGC CCGCAAGAGC AGCGCCTCCG CCCAAAATAA CGCATTAGCG 
GCTGCATCTT CGGCAGCAAC CCCAGCCGGA GGACGCACCA ATGCAGGAGG TGTCAGCACC 
AGTAATAGCG CAAGTGCTGT GCGACACCTA AACAACTCGA CAATCTACGT GGACGACGAG 
AACAGGCCCA AGGGCCATGT AGTCTACTTT ACAGCCAAGC GGAATCTGCC TCCCAAAATG 
TACAAGTGCC ACGAGTGCAG CCCGAACTGC CTCTTTAAGA TCGTTCACCG TCTGGACAGC 
TACAGTCCGT TAGCGAAGCC TCTGCTTTCT GGCTGGGAAC GTCTTGTCAT GCGGCAGAAG 
ACCAAGAAGT CTGTGGTATA CAAGGGACCT TGTGGCAAAA GCTTGCGCAG CCTGGCCGAA 
GTTCACAGGT ACCTTCGAGC TACTGAAAAT GTGTTGAACG TAGACAATTT TGACTTTACG 
CCCGACCTCA AGTGTCTGGC CGAGTACTCC ATTGATCCCT CGATCGTGAA GGACACGGAC 
ATTTCAAAGG GGCAGGAGAA GATGGCGATT CCGCTGGTAA ACTACTACGA CAACACCCTG 
CCGCCCCCAT GCACCTACGC GAAGCAGCGC ATCCCTACCG AGGGTGTTCA TCTGAATCTG 
GACGAGGAGT TCCTCTTGTG CTGCGACTGC GAGGACGACT GCTCGGACAA GTCAAAGTGC 
GCCTGTTGGC AGCTAACGGT GGCGGGCGTG CGGTACTGTA ACCCGAAGAA ACCCATCGAG 
GAGATAGGCT ACCAGTACAA GCGGCTCCAC GAGCACGTGC CCACCGGCAT CTACGAGTGC 
AACTCGCGCT GCAAGTGCAA GAAGAATTGC CTGAACCGGG TGGTGCAGTT CTCCCTAGAG 
ATGAAACTGC AGGTGTTCAA GACCTCAAAT CGTGGATGGG GCTTGCGCTG CGTTAACGAC 
ATCCCCAAGG GTGCTTTCAT CTGTATTTAC GCCGGCCATC TGCTCACGGA GACGATGGCC 
AACGAAGGCG GACAGGACGC CGGCGACGAG TATTTCGCGG ACTTAGATTA CATAGAGGTC 
GCTGAACAGT TAAAAGAGGG ATACGAGTCG GAGGTGGACC ACTCGGACCC GGACGCCGAG 
GAGGACAACG GGGGACCCGA TGCCGAGGAC GATGACGACT TCCGGCCAAA CTATCACTAC 
CAGCGCAAAA TCAAGCGCTC TTCCAGAAGC GGCTCCACGC AGAACAGCTC CACTCAGAGC 
AGCGAGTTGG ACTCGCAGGA GCGTGCGGTG ATCAACTTTA ACCCAAATGC TGATCTGGAC 
GAGACGGTGC GGGAGAACTC GGTGCGGAGG CTTTTCGGCA AGGACGAGGC GCCCTACATC 
ATGGACGCCA AGACAACGGG CAACCTTGGA CGCTATTTCA ACCACTCGTG CAGCCCCAAT 
CTCTTCGTGC AGAACGTGTT CGTGGATACT CACGACCTGC GTTTCCCCTG GGTTGCCTTC 
TTCTCGGCCG CGCACATCCG CTCCGGCACC GAGCTGACTT GGAACTACAA TTACGAGGTG 
GGCGTGGTGC CCGGCAAGGT GTTGTACTGC CAGTGCGGGG CCCCCAATTG CCGCCTTCGT 
CTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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egg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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