Mid1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Mid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa11225 NM_001038883.2
Latest version!
Drosophila melanogaster Mid1, transcript variant A (Mid1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa11225 NM_001274241.1
Latest version!
Drosophila melanogaster Mid1, transcript variant B (Mid1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa11225
    Clone ID Related Accession (Same CDS sequence) NM_001038883.2 , NM_001274241.1
    Accession Version NM_001038883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Mid1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001038883.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCCAG GACCCGGGCT GGTCGTGATG GCGCTTGCGT TTATTTCGGG GGCTCGGCGT 
    GGCAGCAGCA GCAGCATCAG CTCAGCAACA GCAACACCAA CAGCGGCAGC AACATCTGCA
    GCAGCAGCAG CGGCAGCAGC AGCAACATCC GCAGCATCAA CATCCGCAGC AGCAGCAGCA
    GCAGCAAATA CAGCAGGCGG CCGTAAAAGG GGCAGAAGTG GCGGCGGAGT CGGAGGCGGA
    GGAGTTGCAA CCACGTTACC GTCCACTGGC GAGTCGTCGT CATCGTCATT CGTTCGCAGC
    TCTGGCTCAT ACCTGCTGCC CATCTTCCTG CCCCACTACC TCGACTTCGC GCTCCAGTTC
    CACCCCCAAC TGCCGCCGCG GGAGTACAGC CAGGGCCGGG CCCACTCCGA GTCCTGGGCG
    TCCTCGAGGA TAGCGGACAG GCTTCAGCAG CGGCGACGAC GACGCGAGAG CAGCAGCAGC
    AGGGATATAG CAGGTAGGGC AGGCGGAACA GGCGGATCGG GAGCGGATTC AGGAGGTCTG
    GCAGCCACAA CCGGTCACAG CACAGAGTCA AGCGAGGCCA ACAACCAGGA GGACGGCGAG
    GATCGCGGCC TGACAGTGCA GTTCCCCTCG TCGCAGGCCT ACGACGAGGA CCAGGACATC
    TTTGCCCTGG TGGCCGAGGA CGACCTGCTG TCCGAGGAGT CCTTCGATCG CCTGATCAAG
    CTCAACGAAG ACGCCGACGA GGAGGACTAC AATCTGCAGC AGCAGCACTT GCCGCAGCAC
    AAGCAGCAGC AACAGCAACC CGGAGCGGAA ACGGAAATAG ACACAGAGCC AGAGAGCGAG
    ACGGAAACGG AGACGGAGAC AGAGACAGAC ACAGAAGCGG AAGTGGAAGT GGAAAGAGAG
    ACCGAACAGC AGCAGCAGGA CGAGAGCCGA CTGATTGATG AGGGTGTCCT GCAGCACAGC
    GACCACAGTA ACAGCCACTC GAAGGAGTTC GCCATCAGCT ACGATAACAA CAATAACAAA
    CGGGAGGCCA GCGGCCACAT CCTTGATGCC CAGACGGCGC AGTTGATTGG CCACAAGGAT
    AACACCGTGC CCGCCGTGGC CAGCACCGAG TCGGCAGGAG GCGGCAGCAT CACCACATCC
    TTGCCAGCGC CCGTGGATCT GAAGAAGTCC ATACTCGGGA CTGCGACTTC ATTAACACGC
    CTTAATCCTT GGATATCAGC CTGTGATCTG GCACAGCCGG GCACAGGAAC CGACTTGCAG
    GGTCAGTGCT CGGCTGGTAC ACTGCCCATG GCCTGGGTCG ATGAGGGACC CGGGCCCCCA
    ACCTGCCCGC GATCCTGCGC CGAACAGCAG CAACAGAAGC CAGTGGCCTC CAAGGCCAAG
    CGGAGTGCCA CCAGTAACAA TAAAGTCAAG TATTTCGTAA ACTATAAGAC AACCCAGATG
    CGGTTGCGCC GCGCTGGGGA TCACGCGATG GACGCCACCG ACAGCCATTC CCCGCTGGCC
    AGCGAGCGGC CACCGAGCAC CACCGACGAC CAGGACGTGG ACGTGTCCTC CACCACTGAG
    GAGGCGGAGC CGGAGCCCAC GCTGTCCTAC AGCCATCTGC ACGCCCAGGA GCCGGTCCTG
    GCCCACCAGC AGGACCCGCG GGAGCGGGAG CAGGAGCAGC AGGAGCAACA ACAGTGCCTT
    GAATATCTGG GCGATTCCGC CGAGTCGAGT CCGCAGCACC TGTGCGGCCT CCAGTCACCT
    GGTCATCTGC TGGAGCGACT GAAGGTCCTG CGGCTGCGCA ACTGCTGCGA GAGGAGCGTC
    TTTAGCTCCC TGCACACGCT GGCCCTCAAT GCCAGCCTTA CCGATCGCCA GGAGTGCGTC
    CGCGTGCTCA GCGACCTGCT GGACGTAGAT GGACTGGCCA ACAGGATCAC CTGCGAGCTG
    GCCGAGATCC TGTTCCGCTT CGACTGCCGG CAGGTCTACT CGCTGATCAA CCAGTGCGAC
    GACTGCAAGG AAGCATATCG TCGCTGGGTC TGCAGTACAC TAGTGCCATA TTTCGCCGAG
    CCAAAGGACG TGTCGCCGCA ACCGAAGGAC GAAGGACAGG GCAAAGGCAA ACGCTCGGCA
    AGGAGTGCAG CGAGTGCGGA GGCAACGGCG ATGGCGGACG GAATCAAGCA TAAATCTCTG
    AAATTAATCA GCAATAACAA TAAATCAAAT AACGGCAATA ACATGAACGA GCGTCAGATT
    AATCAGAAGC ATGACAAGAG CTCCAATGCG AACGCGAACG CGAACTCAAA CGCCAGCAAG
    GACATTGACC GCAGCGCGGA GGAGGCGATT GACAGGGACC TGGTCCAGAC ATTCTACGAC
    GTTGTTGGTC TGTCTAAGCC CCGCCACGGC GAGGAGGAGG TCCTGCCGGA GAATGAGGCT
    GGATTAGGAC GCAGTGCGGA CTCCGAAGTG GATAAGGGTG CGGAGCCGGA CAGGAAGAGA
    AGTAGGAACA GGGAGGCGGA GCTGGAGCTG GATACGGAGG CGGAGGCCGA ACAACAATCC
    AATAATTTCA TATCGACTGC CAATAATTCG GATCCCAGAG CTACGGATCC AGTGATATCA
    AAACTACAGA AGAGATCAAC GCGCAAGACA GAGTTCCGTA AGCGTCGTCG TATCCGGCCG
    TGCCTGAGCG TCTGCCAAAC CGTGGAACAA AAGTGCCCCT ACCTGCTGCC CGCCGATCGC
    GCCCCTGCGC TGCCCACCCA ATATGCCGGC GAGCCGACAT TTCTCTGCCT AGATCAAAAC
    ATACCCGAGA CTGGGGCGCA GCTGGAGAAG TCTAGCTACG GCCCCAACGA TTGCTGCTAC
    AGTTACTGCA ACGGACCGGC CTCCGGAATA TGCACCGTTT GCCAGGATTT CGGCCAGCCC
    GGGACGGAGG AGCGCGAAAT GGCGGGCAAC TCCACCCGCC GTGTGCACAA CATCACACTC
    TCGCTGAGCT CACATCCCGG CGAGCACGCC CGCGCCAAGA GCGTGGCTTT GGCCCTGCGG
    AACGTCAGCC AAACGGGGAG CGATCCGGAC GCAGTGGAAA CGCTGCTCGA TCGCCTGCCG
    TACTACGCCA GCCACGACGG GATCTTCTAC TACGACGAGG AGGGCGAGAT GCCACACGCG
    TCGCTCTCCG GCGACTGTGC CGTAGTGCCC GCCGCTACCA CGCGCTGCAC CATTCCGTAC
    TACGCCTCCG GGACGGGAGC GGTGGCCAAG CCGCCCACGC AGCTGCTGGT TTGGCTGAGC
    GCCCTGCTCG GCCTGCTCAG CAGCTGTGGA GCGGCGCGTC AGAGGTGGAG CTGTCAGTGG
    AGCAGTGGCT GCGGCGGACA CCGGGCTCGC AGTCGCCAAG CGGTCGCCTG CGAGGCCAAC
    TGCCACGAGC AGGAGCTGGT GAAGAGCAGG AGCTGCAGCC AGAGGAGCCC GAAGGTGCGT
    AGGGAGAAGC CAGGTCAGGA CCAGGACGCT GAGCTGGTGG CCTTCAGGAC GTGGCTGTGG
    TCCAGCTCTT GGCATGCCTA CAAGCTGACC TGCAGCAAAA TACGATACTT CAGCAGCCGG
    AGCCGGTTCA AAGGAGAGAC GAGGGCCGCT CGTTCAGCGA ACTCCAAGGA CCCAAGTGGA
    TTACAGTGGA GTCGGGCGGC AGGATGCACT AGGACTAGGA ACTACCGCTT CTTCTACTAC
    TACAACTACA ACATCAACGA CTGGTGGCGG AGATGGTGGC GCTGCCTGAG CAGCGGGGCC
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001033972.1
    CDS606..4331
    Translation

    Target ORF information:

    RefSeq Version NM_001038883.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Mid1, transcript variant A (Mid1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001038883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCCAG GACCCGGGCT GGTCGTGATG GCGCTTGCGT TTATTTCGGG GGCTCGGCGT 
    GGCAGCAGCA GCAGCATCAG CTCAGCAACA GCAACACCAA CAGCGGCAGC AACATCTGCA
    GCAGCAGCAG CGGCAGCAGC AGCAACATCC GCAGCATCAA CATCCGCAGC AGCAGCAGCA
    GCAGCAAATA CAGCAGGCGG CCGTAAAAGG GGCAGAAGTG GCGGCGGAGT CGGAGGCGGA
    GGAGTTGCAA CCACGTTACC GTCCACTGGC GAGTCGTCGT CATCGTCATT CGTTCGCAGC
    TCTGGCTCAT ACCTGCTGCC CATCTTCCTG CCCCACTACC TCGACTTCGC GCTCCAGTTC
    CACCCCCAAC TGCCGCCGCG GGAGTACAGC CAGGGCCGGG CCCACTCCGA GTCCTGGGCG
    TCCTCGAGGA TAGCGGACAG GCTTCAGCAG CGGCGACGAC GACGCGAGAG CAGCAGCAGC
    AGGGATATAG CAGGTAGGGC AGGCGGAACA GGCGGATCGG GAGCGGATTC AGGAGGTCTG
    GCAGCCACAA CCGGTCACAG CACAGAGTCA AGCGAGGCCA ACAACCAGGA GGACGGCGAG
    GATCGCGGCC TGACAGTGCA GTTCCCCTCG TCGCAGGCCT ACGACGAGGA CCAGGACATC
    TTTGCCCTGG TGGCCGAGGA CGACCTGCTG TCCGAGGAGT CCTTCGATCG CCTGATCAAG
    CTCAACGAAG ACGCCGACGA GGAGGACTAC AATCTGCAGC AGCAGCACTT GCCGCAGCAC
    AAGCAGCAGC AACAGCAACC CGGAGCGGAA ACGGAAATAG ACACAGAGCC AGAGAGCGAG
    ACGGAAACGG AGACGGAGAC AGAGACAGAC ACAGAAGCGG AAGTGGAAGT GGAAAGAGAG
    ACCGAACAGC AGCAGCAGGA CGAGAGCCGA CTGATTGATG AGGGTGTCCT GCAGCACAGC
    GACCACAGTA ACAGCCACTC GAAGGAGTTC GCCATCAGCT ACGATAACAA CAATAACAAA
    CGGGAGGCCA GCGGCCACAT CCTTGATGCC CAGACGGCGC AGTTGATTGG CCACAAGGAT
    AACACCGTGC CCGCCGTGGC CAGCACCGAG TCGGCAGGAG GCGGCAGCAT CACCACATCC
    TTGCCAGCGC CCGTGGATCT GAAGAAGTCC ATACTCGGGA CTGCGACTTC ATTAACACGC
    CTTAATCCTT GGATATCAGC CTGTGATCTG GCACAGCCGG GCACAGGAAC CGACTTGCAG
    GGTCAGTGCT CGGCTGGTAC ACTGCCCATG GCCTGGGTCG ATGAGGGACC CGGGCCCCCA
    ACCTGCCCGC GATCCTGCGC CGAACAGCAG CAACAGAAGC CAGTGGCCTC CAAGGCCAAG
    CGGAGTGCCA CCAGTAACAA TAAAGTCAAG TATTTCGTAA ACTATAAGAC AACCCAGATG
    CGGTTGCGCC GCGCTGGGGA TCACGCGATG GACGCCACCG ACAGCCATTC CCCGCTGGCC
    AGCGAGCGGC CACCGAGCAC CACCGACGAC CAGGACGTGG ACGTGTCCTC CACCACTGAG
    GAGGCGGAGC CGGAGCCCAC GCTGTCCTAC AGCCATCTGC ACGCCCAGGA GCCGGTCCTG
    GCCCACCAGC AGGACCCGCG GGAGCGGGAG CAGGAGCAGC AGGAGCAACA ACAGTGCCTT
    GAATATCTGG GCGATTCCGC CGAGTCGAGT CCGCAGCACC TGTGCGGCCT CCAGTCACCT
    GGTCATCTGC TGGAGCGACT GAAGGTCCTG CGGCTGCGCA ACTGCTGCGA GAGGAGCGTC
    TTTAGCTCCC TGCACACGCT GGCCCTCAAT GCCAGCCTTA CCGATCGCCA GGAGTGCGTC
    CGCGTGCTCA GCGACCTGCT GGACGTAGAT GGACTGGCCA ACAGGATCAC CTGCGAGCTG
    GCCGAGATCC TGTTCCGCTT CGACTGCCGG CAGGTCTACT CGCTGATCAA CCAGTGCGAC
    GACTGCAAGG AAGCATATCG TCGCTGGGTC TGCAGTACAC TAGTGCCATA TTTCGCCGAG
    CCAAAGGACG TGTCGCCGCA ACCGAAGGAC GAAGGACAGG GCAAAGGCAA ACGCTCGGCA
    AGGAGTGCAG CGAGTGCGGA GGCAACGGCG ATGGCGGACG GAATCAAGCA TAAATCTCTG
    AAATTAATCA GCAATAACAA TAAATCAAAT AACGGCAATA ACATGAACGA GCGTCAGATT
    AATCAGAAGC ATGACAAGAG CTCCAATGCG AACGCGAACG CGAACTCAAA CGCCAGCAAG
    GACATTGACC GCAGCGCGGA GGAGGCGATT GACAGGGACC TGGTCCAGAC ATTCTACGAC
    GTTGTTGGTC TGTCTAAGCC CCGCCACGGC GAGGAGGAGG TCCTGCCGGA GAATGAGGCT
    GGATTAGGAC GCAGTGCGGA CTCCGAAGTG GATAAGGGTG CGGAGCCGGA CAGGAAGAGA
    AGTAGGAACA GGGAGGCGGA GCTGGAGCTG GATACGGAGG CGGAGGCCGA ACAACAATCC
    AATAATTTCA TATCGACTGC CAATAATTCG GATCCCAGAG CTACGGATCC AGTGATATCA
    AAACTACAGA AGAGATCAAC GCGCAAGACA GAGTTCCGTA AGCGTCGTCG TATCCGGCCG
    TGCCTGAGCG TCTGCCAAAC CGTGGAACAA AAGTGCCCCT ACCTGCTGCC CGCCGATCGC
    GCCCCTGCGC TGCCCACCCA ATATGCCGGC GAGCCGACAT TTCTCTGCCT AGATCAAAAC
    ATACCCGAGA CTGGGGCGCA GCTGGAGAAG TCTAGCTACG GCCCCAACGA TTGCTGCTAC
    AGTTACTGCA ACGGACCGGC CTCCGGAATA TGCACCGTTT GCCAGGATTT CGGCCAGCCC
    GGGACGGAGG AGCGCGAAAT GGCGGGCAAC TCCACCCGCC GTGTGCACAA CATCACACTC
    TCGCTGAGCT CACATCCCGG CGAGCACGCC CGCGCCAAGA GCGTGGCTTT GGCCCTGCGG
    AACGTCAGCC AAACGGGGAG CGATCCGGAC GCAGTGGAAA CGCTGCTCGA TCGCCTGCCG
    TACTACGCCA GCCACGACGG GATCTTCTAC TACGACGAGG AGGGCGAGAT GCCACACGCG
    TCGCTCTCCG GCGACTGTGC CGTAGTGCCC GCCGCTACCA CGCGCTGCAC CATTCCGTAC
    TACGCCTCCG GGACGGGAGC GGTGGCCAAG CCGCCCACGC AGCTGCTGGT TTGGCTGAGC
    GCCCTGCTCG GCCTGCTCAG CAGCTGTGGA GCGGCGCGTC AGAGGTGGAG CTGTCAGTGG
    AGCAGTGGCT GCGGCGGACA CCGGGCTCGC AGTCGCCAAG CGGTCGCCTG CGAGGCCAAC
    TGCCACGAGC AGGAGCTGGT GAAGAGCAGG AGCTGCAGCC AGAGGAGCCC GAAGGTGCGT
    AGGGAGAAGC CAGGTCAGGA CCAGGACGCT GAGCTGGTGG CCTTCAGGAC GTGGCTGTGG
    TCCAGCTCTT GGCATGCCTA CAAGCTGACC TGCAGCAAAA TACGATACTT CAGCAGCCGG
    AGCCGGTTCA AAGGAGAGAC GAGGGCCGCT CGTTCAGCGA ACTCCAAGGA CCCAAGTGGA
    TTACAGTGGA GTCGGGCGGC AGGATGCACT AGGACTAGGA ACTACCGCTT CTTCTACTAC
    TACAACTACA ACATCAACGA CTGGTGGCGG AGATGGTGGC GCTGCCTGAG CAGCGGGGCC
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11225
    Clone ID Related Accession (Same CDS sequence) NM_001038883.2 , NM_001274241.1
    Accession Version NM_001274241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Mid1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCCAG GACCCGGGCT GGTCGTGATG GCGCTTGCGT TTATTTCGGG GGCTCGGCGT 
    GGCAGCAGCA GCAGCATCAG CTCAGCAACA GCAACACCAA CAGCGGCAGC AACATCTGCA
    GCAGCAGCAG CGGCAGCAGC AGCAACATCC GCAGCATCAA CATCCGCAGC AGCAGCAGCA
    GCAGCAAATA CAGCAGGCGG CCGTAAAAGG GGCAGAAGTG GCGGCGGAGT CGGAGGCGGA
    GGAGTTGCAA CCACGTTACC GTCCACTGGC GAGTCGTCGT CATCGTCATT CGTTCGCAGC
    TCTGGCTCAT ACCTGCTGCC CATCTTCCTG CCCCACTACC TCGACTTCGC GCTCCAGTTC
    CACCCCCAAC TGCCGCCGCG GGAGTACAGC CAGGGCCGGG CCCACTCCGA GTCCTGGGCG
    TCCTCGAGGA TAGCGGACAG GCTTCAGCAG CGGCGACGAC GACGCGAGAG CAGCAGCAGC
    AGGGATATAG CAGGTAGGGC AGGCGGAACA GGCGGATCGG GAGCGGATTC AGGAGGTCTG
    GCAGCCACAA CCGGTCACAG CACAGAGTCA AGCGAGGCCA ACAACCAGGA GGACGGCGAG
    GATCGCGGCC TGACAGTGCA GTTCCCCTCG TCGCAGGCCT ACGACGAGGA CCAGGACATC
    TTTGCCCTGG TGGCCGAGGA CGACCTGCTG TCCGAGGAGT CCTTCGATCG CCTGATCAAG
    CTCAACGAAG ACGCCGACGA GGAGGACTAC AATCTGCAGC AGCAGCACTT GCCGCAGCAC
    AAGCAGCAGC AACAGCAACC CGGAGCGGAA ACGGAAATAG ACACAGAGCC AGAGAGCGAG
    ACGGAAACGG AGACGGAGAC AGAGACAGAC ACAGAAGCGG AAGTGGAAGT GGAAAGAGAG
    ACCGAACAGC AGCAGCAGGA CGAGAGCCGA CTGATTGATG AGGGTGTCCT GCAGCACAGC
    GACCACAGTA ACAGCCACTC GAAGGAGTTC GCCATCAGCT ACGATAACAA CAATAACAAA
    CGGGAGGCCA GCGGCCACAT CCTTGATGCC CAGACGGCGC AGTTGATTGG CCACAAGGAT
    AACACCGTGC CCGCCGTGGC CAGCACCGAG TCGGCAGGAG GCGGCAGCAT CACCACATCC
    TTGCCAGCGC CCGTGGATCT GAAGAAGTCC ATACTCGGGA CTGCGACTTC ATTAACACGC
    CTTAATCCTT GGATATCAGC CTGTGATCTG GCACAGCCGG GCACAGGAAC CGACTTGCAG
    GGTCAGTGCT CGGCTGGTAC ACTGCCCATG GCCTGGGTCG ATGAGGGACC CGGGCCCCCA
    ACCTGCCCGC GATCCTGCGC CGAACAGCAG CAACAGAAGC CAGTGGCCTC CAAGGCCAAG
    CGGAGTGCCA CCAGTAACAA TAAAGTCAAG TATTTCGTAA ACTATAAGAC AACCCAGATG
    CGGTTGCGCC GCGCTGGGGA TCACGCGATG GACGCCACCG ACAGCCATTC CCCGCTGGCC
    AGCGAGCGGC CACCGAGCAC CACCGACGAC CAGGACGTGG ACGTGTCCTC CACCACTGAG
    GAGGCGGAGC CGGAGCCCAC GCTGTCCTAC AGCCATCTGC ACGCCCAGGA GCCGGTCCTG
    GCCCACCAGC AGGACCCGCG GGAGCGGGAG CAGGAGCAGC AGGAGCAACA ACAGTGCCTT
    GAATATCTGG GCGATTCCGC CGAGTCGAGT CCGCAGCACC TGTGCGGCCT CCAGTCACCT
    GGTCATCTGC TGGAGCGACT GAAGGTCCTG CGGCTGCGCA ACTGCTGCGA GAGGAGCGTC
    TTTAGCTCCC TGCACACGCT GGCCCTCAAT GCCAGCCTTA CCGATCGCCA GGAGTGCGTC
    CGCGTGCTCA GCGACCTGCT GGACGTAGAT GGACTGGCCA ACAGGATCAC CTGCGAGCTG
    GCCGAGATCC TGTTCCGCTT CGACTGCCGG CAGGTCTACT CGCTGATCAA CCAGTGCGAC
    GACTGCAAGG AAGCATATCG TCGCTGGGTC TGCAGTACAC TAGTGCCATA TTTCGCCGAG
    CCAAAGGACG TGTCGCCGCA ACCGAAGGAC GAAGGACAGG GCAAAGGCAA ACGCTCGGCA
    AGGAGTGCAG CGAGTGCGGA GGCAACGGCG ATGGCGGACG GAATCAAGCA TAAATCTCTG
    AAATTAATCA GCAATAACAA TAAATCAAAT AACGGCAATA ACATGAACGA GCGTCAGATT
    AATCAGAAGC ATGACAAGAG CTCCAATGCG AACGCGAACG CGAACTCAAA CGCCAGCAAG
    GACATTGACC GCAGCGCGGA GGAGGCGATT GACAGGGACC TGGTCCAGAC ATTCTACGAC
    GTTGTTGGTC TGTCTAAGCC CCGCCACGGC GAGGAGGAGG TCCTGCCGGA GAATGAGGCT
    GGATTAGGAC GCAGTGCGGA CTCCGAAGTG GATAAGGGTG CGGAGCCGGA CAGGAAGAGA
    AGTAGGAACA GGGAGGCGGA GCTGGAGCTG GATACGGAGG CGGAGGCCGA ACAACAATCC
    AATAATTTCA TATCGACTGC CAATAATTCG GATCCCAGAG CTACGGATCC AGTGATATCA
    AAACTACAGA AGAGATCAAC GCGCAAGACA GAGTTCCGTA AGCGTCGTCG TATCCGGCCG
    TGCCTGAGCG TCTGCCAAAC CGTGGAACAA AAGTGCCCCT ACCTGCTGCC CGCCGATCGC
    GCCCCTGCGC TGCCCACCCA ATATGCCGGC GAGCCGACAT TTCTCTGCCT AGATCAAAAC
    ATACCCGAGA CTGGGGCGCA GCTGGAGAAG TCTAGCTACG GCCCCAACGA TTGCTGCTAC
    AGTTACTGCA ACGGACCGGC CTCCGGAATA TGCACCGTTT GCCAGGATTT CGGCCAGCCC
    GGGACGGAGG AGCGCGAAAT GGCGGGCAAC TCCACCCGCC GTGTGCACAA CATCACACTC
    TCGCTGAGCT CACATCCCGG CGAGCACGCC CGCGCCAAGA GCGTGGCTTT GGCCCTGCGG
    AACGTCAGCC AAACGGGGAG CGATCCGGAC GCAGTGGAAA CGCTGCTCGA TCGCCTGCCG
    TACTACGCCA GCCACGACGG GATCTTCTAC TACGACGAGG AGGGCGAGAT GCCACACGCG
    TCGCTCTCCG GCGACTGTGC CGTAGTGCCC GCCGCTACCA CGCGCTGCAC CATTCCGTAC
    TACGCCTCCG GGACGGGAGC GGTGGCCAAG CCGCCCACGC AGCTGCTGGT TTGGCTGAGC
    GCCCTGCTCG GCCTGCTCAG CAGCTGTGGA GCGGCGCGTC AGAGGTGGAG CTGTCAGTGG
    AGCAGTGGCT GCGGCGGACA CCGGGCTCGC AGTCGCCAAG CGGTCGCCTG CGAGGCCAAC
    TGCCACGAGC AGGAGCTGGT GAAGAGCAGG AGCTGCAGCC AGAGGAGCCC GAAGGTGCGT
    AGGGAGAAGC CAGGTCAGGA CCAGGACGCT GAGCTGGTGG CCTTCAGGAC GTGGCTGTGG
    TCCAGCTCTT GGCATGCCTA CAAGCTGACC TGCAGCAAAA TACGATACTT CAGCAGCCGG
    AGCCGGTTCA AAGGAGAGAC GAGGGCCGCT CGTTCAGCGA ACTCCAAGGA CCCAAGTGGA
    TTACAGTGGA GTCGGGCGGC AGGATGCACT AGGACTAGGA ACTACCGCTT CTTCTACTAC
    TACAACTACA ACATCAACGA CTGGTGGCGG AGATGGTGGC GCTGCCTGAG CAGCGGGGCC
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261170.1
    CDS606..4331
    Translation

    Target ORF information:

    RefSeq Version NM_001274241.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Mid1, transcript variant B (Mid1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCCAG GACCCGGGCT GGTCGTGATG GCGCTTGCGT TTATTTCGGG GGCTCGGCGT 
    GGCAGCAGCA GCAGCATCAG CTCAGCAACA GCAACACCAA CAGCGGCAGC AACATCTGCA
    GCAGCAGCAG CGGCAGCAGC AGCAACATCC GCAGCATCAA CATCCGCAGC AGCAGCAGCA
    GCAGCAAATA CAGCAGGCGG CCGTAAAAGG GGCAGAAGTG GCGGCGGAGT CGGAGGCGGA
    GGAGTTGCAA CCACGTTACC GTCCACTGGC GAGTCGTCGT CATCGTCATT CGTTCGCAGC
    TCTGGCTCAT ACCTGCTGCC CATCTTCCTG CCCCACTACC TCGACTTCGC GCTCCAGTTC
    CACCCCCAAC TGCCGCCGCG GGAGTACAGC CAGGGCCGGG CCCACTCCGA GTCCTGGGCG
    TCCTCGAGGA TAGCGGACAG GCTTCAGCAG CGGCGACGAC GACGCGAGAG CAGCAGCAGC
    AGGGATATAG CAGGTAGGGC AGGCGGAACA GGCGGATCGG GAGCGGATTC AGGAGGTCTG
    GCAGCCACAA CCGGTCACAG CACAGAGTCA AGCGAGGCCA ACAACCAGGA GGACGGCGAG
    GATCGCGGCC TGACAGTGCA GTTCCCCTCG TCGCAGGCCT ACGACGAGGA CCAGGACATC
    TTTGCCCTGG TGGCCGAGGA CGACCTGCTG TCCGAGGAGT CCTTCGATCG CCTGATCAAG
    CTCAACGAAG ACGCCGACGA GGAGGACTAC AATCTGCAGC AGCAGCACTT GCCGCAGCAC
    AAGCAGCAGC AACAGCAACC CGGAGCGGAA ACGGAAATAG ACACAGAGCC AGAGAGCGAG
    ACGGAAACGG AGACGGAGAC AGAGACAGAC ACAGAAGCGG AAGTGGAAGT GGAAAGAGAG
    ACCGAACAGC AGCAGCAGGA CGAGAGCCGA CTGATTGATG AGGGTGTCCT GCAGCACAGC
    GACCACAGTA ACAGCCACTC GAAGGAGTTC GCCATCAGCT ACGATAACAA CAATAACAAA
    CGGGAGGCCA GCGGCCACAT CCTTGATGCC CAGACGGCGC AGTTGATTGG CCACAAGGAT
    AACACCGTGC CCGCCGTGGC CAGCACCGAG TCGGCAGGAG GCGGCAGCAT CACCACATCC
    TTGCCAGCGC CCGTGGATCT GAAGAAGTCC ATACTCGGGA CTGCGACTTC ATTAACACGC
    CTTAATCCTT GGATATCAGC CTGTGATCTG GCACAGCCGG GCACAGGAAC CGACTTGCAG
    GGTCAGTGCT CGGCTGGTAC ACTGCCCATG GCCTGGGTCG ATGAGGGACC CGGGCCCCCA
    ACCTGCCCGC GATCCTGCGC CGAACAGCAG CAACAGAAGC CAGTGGCCTC CAAGGCCAAG
    CGGAGTGCCA CCAGTAACAA TAAAGTCAAG TATTTCGTAA ACTATAAGAC AACCCAGATG
    CGGTTGCGCC GCGCTGGGGA TCACGCGATG GACGCCACCG ACAGCCATTC CCCGCTGGCC
    AGCGAGCGGC CACCGAGCAC CACCGACGAC CAGGACGTGG ACGTGTCCTC CACCACTGAG
    GAGGCGGAGC CGGAGCCCAC GCTGTCCTAC AGCCATCTGC ACGCCCAGGA GCCGGTCCTG
    GCCCACCAGC AGGACCCGCG GGAGCGGGAG CAGGAGCAGC AGGAGCAACA ACAGTGCCTT
    GAATATCTGG GCGATTCCGC CGAGTCGAGT CCGCAGCACC TGTGCGGCCT CCAGTCACCT
    GGTCATCTGC TGGAGCGACT GAAGGTCCTG CGGCTGCGCA ACTGCTGCGA GAGGAGCGTC
    TTTAGCTCCC TGCACACGCT GGCCCTCAAT GCCAGCCTTA CCGATCGCCA GGAGTGCGTC
    CGCGTGCTCA GCGACCTGCT GGACGTAGAT GGACTGGCCA ACAGGATCAC CTGCGAGCTG
    GCCGAGATCC TGTTCCGCTT CGACTGCCGG CAGGTCTACT CGCTGATCAA CCAGTGCGAC
    GACTGCAAGG AAGCATATCG TCGCTGGGTC TGCAGTACAC TAGTGCCATA TTTCGCCGAG
    CCAAAGGACG TGTCGCCGCA ACCGAAGGAC GAAGGACAGG GCAAAGGCAA ACGCTCGGCA
    AGGAGTGCAG CGAGTGCGGA GGCAACGGCG ATGGCGGACG GAATCAAGCA TAAATCTCTG
    AAATTAATCA GCAATAACAA TAAATCAAAT AACGGCAATA ACATGAACGA GCGTCAGATT
    AATCAGAAGC ATGACAAGAG CTCCAATGCG AACGCGAACG CGAACTCAAA CGCCAGCAAG
    GACATTGACC GCAGCGCGGA GGAGGCGATT GACAGGGACC TGGTCCAGAC ATTCTACGAC
    GTTGTTGGTC TGTCTAAGCC CCGCCACGGC GAGGAGGAGG TCCTGCCGGA GAATGAGGCT
    GGATTAGGAC GCAGTGCGGA CTCCGAAGTG GATAAGGGTG CGGAGCCGGA CAGGAAGAGA
    AGTAGGAACA GGGAGGCGGA GCTGGAGCTG GATACGGAGG CGGAGGCCGA ACAACAATCC
    AATAATTTCA TATCGACTGC CAATAATTCG GATCCCAGAG CTACGGATCC AGTGATATCA
    AAACTACAGA AGAGATCAAC GCGCAAGACA GAGTTCCGTA AGCGTCGTCG TATCCGGCCG
    TGCCTGAGCG TCTGCCAAAC CGTGGAACAA AAGTGCCCCT ACCTGCTGCC CGCCGATCGC
    GCCCCTGCGC TGCCCACCCA ATATGCCGGC GAGCCGACAT TTCTCTGCCT AGATCAAAAC
    ATACCCGAGA CTGGGGCGCA GCTGGAGAAG TCTAGCTACG GCCCCAACGA TTGCTGCTAC
    AGTTACTGCA ACGGACCGGC CTCCGGAATA TGCACCGTTT GCCAGGATTT CGGCCAGCCC
    GGGACGGAGG AGCGCGAAAT GGCGGGCAAC TCCACCCGCC GTGTGCACAA CATCACACTC
    TCGCTGAGCT CACATCCCGG CGAGCACGCC CGCGCCAAGA GCGTGGCTTT GGCCCTGCGG
    AACGTCAGCC AAACGGGGAG CGATCCGGAC GCAGTGGAAA CGCTGCTCGA TCGCCTGCCG
    TACTACGCCA GCCACGACGG GATCTTCTAC TACGACGAGG AGGGCGAGAT GCCACACGCG
    TCGCTCTCCG GCGACTGTGC CGTAGTGCCC GCCGCTACCA CGCGCTGCAC CATTCCGTAC
    TACGCCTCCG GGACGGGAGC GGTGGCCAAG CCGCCCACGC AGCTGCTGGT TTGGCTGAGC
    GCCCTGCTCG GCCTGCTCAG CAGCTGTGGA GCGGCGCGTC AGAGGTGGAG CTGTCAGTGG
    AGCAGTGGCT GCGGCGGACA CCGGGCTCGC AGTCGCCAAG CGGTCGCCTG CGAGGCCAAC
    TGCCACGAGC AGGAGCTGGT GAAGAGCAGG AGCTGCAGCC AGAGGAGCCC GAAGGTGCGT
    AGGGAGAAGC CAGGTCAGGA CCAGGACGCT GAGCTGGTGG CCTTCAGGAC GTGGCTGTGG
    TCCAGCTCTT GGCATGCCTA CAAGCTGACC TGCAGCAAAA TACGATACTT CAGCAGCCGG
    AGCCGGTTCA AAGGAGAGAC GAGGGCCGCT CGTTCAGCGA ACTCCAAGGA CCCAAGTGGA
    TTACAGTGGA GTCGGGCGGC AGGATGCACT AGGACTAGGA ACTACCGCTT CTTCTACTAC
    TACAACTACA ACATCAACGA CTGGTGGCGG AGATGGTGGC GCTGCCTGAG CAGCGGGGCC
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.