MAN1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN1
Entrez Gene ID	37838
Full Name	CG3167 gene product from transcript CG3167-RC
Synonyms	BcDNA:LD24895,CG3167,Dmel\CG3167,dMAN1,dMan1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B5-60B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299883.1	NP_001286812.1	MAN1, isoform C
NM_166651.2	NP_726404.1	MAN1, isoform A
NM_138029.3	NP_611873.1	MAN1, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007422	XP_001687932.1
Drosophila melanogaster (fruit fly)	MAN1	NP_611873.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

neither Bocks nor Ote rescued dMAN1-/- mutant phenotypes, implying that dMAN1 makes unique contributions to wing development
Title: Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.

Genetic interaction experiments suggest that MAN1 is a BMP signaling antagonist both at the neuromuscular junction and during crossvein formation
Title: The Drosophila LEM-domain protein MAN1 antagonizes BMP signaling at the neuromuscular junction and the wing crossveins.

Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein
Title: Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein.

description of the Drosophila melanogaster LEM-domain protein encoded by the gene CG3167 which is the putative ortholog to vertebrate MAN1; concluded that MAN1 protein is not a limiting component of the nuclear architecture in Drosophila cultured cells
Title: The Drosophila melanogaster LEM-domain protein MAN1.

The following MAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11102	NM_001299883.1 Latest version!	Drosophila melanogaster MAN1, transcript variant C (MAN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11102	NM_166651.2 Latest version!	Drosophila melanogaster MAN1, transcript variant A (MAN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11102	NM_138029.3 Latest version!	Drosophila melanogaster MAN1, transcript variant B (MAN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN1 ( NM_001299883.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286812.1 Drosophila melanogaster MAN1, transcript variant C (MAN1), mRNA.

Price & Availability↑

CloneID	OFa11102
Clone ID Related Accession (Same CDS sequence)	NM_001299883.1 , NM_166651.2 , NM_138029.3
Accession Version	NM_001299883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAN1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286812.1
CDS	470..2422
Translation	MSTESLNSLSDKELHRKLIQSGFPSTPVTETTRAVLIEKLRKHTRADKLKKRSNKYVLYSKEQQESPPFPQYHQYHAPQPPQNYANGLDNNNDLDQTGGSSAYNRSLDESDSSPLQLSASKMYAPPPVVASNYDGDCSPHSLGLNGKYLQPCSMPYAIDTSNNYGKPSGKAKLSDGGVVNRLLSFRDTTIQRKFNYPTGQASRIPLRKERLTRFALSDLKSFIRNPDIRPYVIPRVLISLFLIFLTIITVLYVGKRFEQSPIDKAALKYTLCNPNDMQMISEKVNCIEKDSLRGALDMSEELFRHLNERARLHHCKDANLSPALEIGEFVREMVSNPKTHRGNLHSNLMAAKYLITENPQWSIQVVDSTKHLGQTSHFELSEPNLPLKCIVLKKVTRFFTVIGALLLIVAGFLIVYVAVVIYRVKQKEALLAVDQFQKDIINELIYLSSQSESPEVVINQLQEKFLPAKKRSKLLSSWNKALKQLEKNDSRVLFGMVNRDGKAMRTIAWNRNVDKKDVGLVKKWQSPAFDNSNKIANPPTPCLKIRHMFDSSEVDQANLKQSIVESIIEKVGTRCKICDVQLDVQSCCVYIRCASEEDAGTIHKEINGWWFDKRLISIKFLRLERYLSRFPKPSAEPLYFHTNEAANTHS

Target ORF information:

RefSeq Version	NM_001299883.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAN1, transcript variant C (MAN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299883.1

ORF Insert Sequence:

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1 ( NM_166651.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726404.1 Drosophila melanogaster MAN1, transcript variant A (MAN1), mRNA.

Price & Availability↑

CloneID	OFa11102
Clone ID Related Accession (Same CDS sequence)	NM_001299883.1 , NM_166651.2 , NM_138029.3
Accession Version	NM_166651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAN1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166651.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726404.1
CDS	219..2171
Translation	MSTESLNSLSDKELHRKLIQSGFPSTPVTETTRAVLIEKLRKHTRADKLKKRSNKYVLYSKEQQESPPFPQYHQYHAPQPPQNYANGLDNNNDLDQTGGSSAYNRSLDESDSSPLQLSASKMYAPPPVVASNYDGDCSPHSLGLNGKYLQPCSMPYAIDTSNNYGKPSGKAKLSDGGVVNRLLSFRDTTIQRKFNYPTGQASRIPLRKERLTRFALSDLKSFIRNPDIRPYVIPRVLISLFLIFLTIITVLYVGKRFEQSPIDKAALKYTLCNPNDMQMISEKVNCIEKDSLRGALDMSEELFRHLNERARLHHCKDANLSPALEIGEFVREMVSNPKTHRGNLHSNLMAAKYLITENPQWSIQVVDSTKHLGQTSHFELSEPNLPLKCIVLKKVTRFFTVIGALLLIVAGFLIVYVAVVIYRVKQKEALLAVDQFQKDIINELIYLSSQSESPEVVINQLQEKFLPAKKRSKLLSSWNKALKQLEKNDSRVLFGMVNRDGKAMRTIAWNRNVDKKDVGLVKKWQSPAFDNSNKIANPPTPCLKIRHMFDSSEVDQANLKQSIVESIIEKVGTRCKICDVQLDVQSCCVYIRCASEEDAGTIHKEINGWWFDKRLISIKFLRLERYLSRFPKPSAEPLYFHTNEAANTHS

Target ORF information:

RefSeq Version	NM_166651.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAN1, transcript variant A (MAN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166651.2

ORF Insert Sequence:

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1 ( NM_138029.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611873.1 Drosophila melanogaster MAN1, transcript variant B (MAN1), mRNA.

Price & Availability↑

CloneID	OFa11102
Clone ID Related Accession (Same CDS sequence)	NM_001299883.1 , NM_166651.2 , NM_138029.3
Accession Version	NM_138029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MAN1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138029.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611873.1
CDS	207..2159
Translation	MSTESLNSLSDKELHRKLIQSGFPSTPVTETTRAVLIEKLRKHTRADKLKKRSNKYVLYSKEQQESPPFPQYHQYHAPQPPQNYANGLDNNNDLDQTGGSSAYNRSLDESDSSPLQLSASKMYAPPPVVASNYDGDCSPHSLGLNGKYLQPCSMPYAIDTSNNYGKPSGKAKLSDGGVVNRLLSFRDTTIQRKFNYPTGQASRIPLRKERLTRFALSDLKSFIRNPDIRPYVIPRVLISLFLIFLTIITVLYVGKRFEQSPIDKAALKYTLCNPNDMQMISEKVNCIEKDSLRGALDMSEELFRHLNERARLHHCKDANLSPALEIGEFVREMVSNPKTHRGNLHSNLMAAKYLITENPQWSIQVVDSTKHLGQTSHFELSEPNLPLKCIVLKKVTRFFTVIGALLLIVAGFLIVYVAVVIYRVKQKEALLAVDQFQKDIINELIYLSSQSESPEVVINQLQEKFLPAKKRSKLLSSWNKALKQLEKNDSRVLFGMVNRDGKAMRTIAWNRNVDKKDVGLVKKWQSPAFDNSNKIANPPTPCLKIRHMFDSSEVDQANLKQSIVESIIEKVGTRCKICDVQLDVQSCCVYIRCASEEDAGTIHKEINGWWFDKRLISIKFLRLERYLSRFPKPSAEPLYFHTNEAANTHS

Target ORF information:

RefSeq Version	NM_138029.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAN1, transcript variant B (MAN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138029.3

ORF Insert Sequence:

ATGTCAACCG AGAGTTTGAA CAGTTTGAGC GACAAGGAAC TGCACAGGAA GCTAATCCAG 
AGCGGCTTCC CCAGCACTCC GGTGACGGAA ACAACGCGAG CGGTCCTCAT CGAGAAGTTG 
AGAAAGCACA CGCGAGCCGA CAAGCTGAAA AAGAGGAGCA ACAAGTACGT GCTGTACTCC 
AAGGAGCAGC AAGAGTCGCC CCCATTTCCA CAGTACCACC AGTACCACGC CCCACAACCG 
CCCCAAAACT ACGCCAATGG CCTTGATAAC AATAACGACC TGGACCAGAC CGGAGGCTCC 
TCAGCTTACA ATCGCAGTCT CGATGAGAGC GACTCAAGCC CGCTTCAGTT GTCCGCCTCC 
AAAATGTACG CCCCACCACC GGTTGTGGCT TCCAATTACG ATGGAGACTG CTCGCCGCAC 
AGCTTGGGGT TAAATGGCAA ATACCTGCAG CCATGCTCCA TGCCGTACGC CATAGACACG 
TCGAATAACT ATGGCAAACC GAGCGGAAAA GCTAAGTTAT CTGACGGAGG CGTGGTAAAT 
AGACTGCTCA GCTTTCGGGA TACCACGATC CAGCGGAAGT TCAACTATCC CACTGGCCAG 
GCGTCGAGAA TCCCGCTCAG GAAAGAAAGG CTAACTCGGT TCGCTTTGTC GGACCTTAAA 
TCCTTCATCA GGAATCCCGA CATCAGGCCC TATGTAATAC CACGAGTGCT AATTTCGCTG 
TTCTTAATAT TTTTGACAAT AATCACTGTC CTCTATGTGG GCAAGAGGTT CGAGCAGAGC 
CCCATCGATA AGGCCGCACT GAAGTATACA TTATGTAACC CCAACGATAT GCAAATGATC 
AGCGAAAAGG TGAATTGCAT CGAAAAAGAT TCGCTAAGGG GTGCGCTGGA CATGAGCGAG 
GAACTCTTTA GGCATCTGAA CGAGCGAGCC AGGCTCCATC ACTGCAAAGA CGCCAATTTG 
TCGCCCGCTT TGGAAATAGG AGAGTTTGTG CGGGAAATGG TCAGCAACCC AAAGACTCAC 
AGGGGAAATC TGCACAGCAA CTTGATGGCT GCCAAATATT TGATTACCGA GAATCCGCAG 
TGGAGCATCC AAGTGGTGGA TTCAACGAAG CACCTTGGAC AGACCTCGCA CTTCGAGCTT 
TCTGAACCCA ATCTGCCGCT CAAGTGCATT GTGTTGAAGA AAGTTACCAG GTTTTTCACC 
GTTATCGGTG CACTTCTCTT AATAGTCGCT GGATTTCTCA TCGTGTACGT CGCGGTAGTA 
ATATACCGAG TTAAGCAGAA GGAAGCCCTG TTGGCGGTCG ATCAGTTCCA AAAGGACATA 
ATAAACGAGC TAATTTACTT GAGCTCCCAG TCCGAGAGTC CCGAGGTAGT TATTAACCAG 
CTGCAGGAGA AGTTTCTGCC GGCCAAGAAG CGCTCAAAGC TCTTGTCTTC TTGGAACAAG 
GCCCTCAAGC AGCTGGAGAA AAACGATAGC CGTGTGCTGT TCGGAATGGT GAATCGTGAC 
GGGAAGGCAA TGCGCACAAT TGCGTGGAAC AGGAACGTTG ACAAAAAGGA TGTGGGGCTT 
GTTAAGAAGT GGCAGAGTCC CGCCTTCGAT AACTCGAACA AGATTGCCAA CCCGCCGACT 
CCCTGCCTGA AGATTAGGCA CATGTTCGAC TCCTCAGAGG TGGATCAGGC CAATCTGAAG 
CAGTCCATCG TGGAGTCGAT CATCGAGAAG GTCGGCACGC GCTGCAAGAT CTGCGATGTC 
CAGCTGGACG TGCAGTCCTG CTGCGTGTAC ATCCGCTGCG CCAGCGAGGA GGACGCCGGC 
ACGATTCACA AAGAGATCAA CGGCTGGTGG TTCGACAAGC GCCTGATTTC GATTAAATTC 
TTAAGACTGG AACGCTACCT GAGCCGTTTC CCCAAGCCCT CGGCCGAGCC ACTTTACTTT 
CACACCAACG AGGCAGCCAA CACTCACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MAN1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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