egl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	egl
Entrez Gene ID	37757
Full Name	egalitarian
Synonyms	CG4051,Dmel\CG4051,EGL,Egl,egal
General protein information	Preferred Names egalitarian Names CG4051 gene product from transcript CG4051-RB CG4051-PB CG4051-PC egl-PB egl-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59F7-59F7\|2-105 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299850.1	NP_001286779.1	egalitarian, isoform C
NM_166623.3	NP_726360.3	egalitarian, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005155	XP_314043.4
Drosophila melanogaster (fruit fly)	egl	NP_726360.3
Caenorhabditis elegans (roundworm)	egal-1	NP_001021323.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the absence of Egl function, oocyte microtubules completely fail to reorganize. Egl also appears to function in regulating the translation of critical polarity determining mRNAs.
Title: Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber.

direct recognition of these mRNA localization signals is mediated by the Egalitarian (Egl) protein
Title: Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor.

Egl interacts directly with dynein light chain, a microtubule motor component, through an Egl domain distinct from that which binds BicD; data provide a direct link between a molecule necessary for oocyte specification and the microtubule motor complex
Title: Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate.

Through the combined actions of the fusome, MT, and Baz, Par-1 is selectively enriched and localized within the oocyte, where, in conjunction with BicD, Egalitarian (Egl), and Dynein, it acts on the MT cytoskeleton to effect polarization.
Title: The fusome and microtubules enrich Par-1 in the oocyte, where it effects polarization in conjunction with Par-3, BicD, Egl, and dynein.

component of a selective dynein motor complex that drives transcript localization in a variety of tissues
Title: Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis.

The following egl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13171	NM_001299850.1 Latest version!	Drosophila melanogaster egalitarian (egl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa13171	NM_166623.3 Latest version!	Drosophila melanogaster egalitarian (egl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egl ( NM_001299850.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286779.1 Drosophila melanogaster egalitarian (egl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13171
Clone ID Related Accession (Same CDS sequence)	NM_001299850.1 , NM_166623.3
Accession Version	NM_001299850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	egalitarian, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCCA TGGAGTACGA GATGGCACGC AACATGACGT TGCTCTTCTT CCTGGAGCGA 
CTGCTGGACA AGGGCGAGCC CCGCACCGTC CACGACCTGT CCTGCCAATT CGGCAACAAG 
GAGTTCACCA AGGAGATGCG CCAAATCGCC GGTGGCAGTC AGTCGGGTCT GAAGAAGTTC 
CTCGCCCAGT ACCCGGCCAT ATTTCTGGTT GACGGCGACT ACGTCCAGGT GAACGCCTAT 
CAGCACCACA ACGCGGACGA TGGCGGCTGC GGGGGCAAGC GGGACTACAT CCAAGAGGCT 
AAAGACTACT TCAAGAACAA GATGCTGCAG TACGGAGCGG CTGCCGAGGT GCCCGTCCGC 
AGTCTCCTGG GCCACCGGTC GCAGGCGTCG CCCCAAGTGC GTCACATATC GGGCCAGCAC 
ATCAAGGAGT TCACCGACTT CCTGATGAAG CACACGGACA CCTTCAAGGT GACGGACGAC 
TATGTGATGC TGGTGGGCTG CGAGAACATG ACGGATCTGC CGGCGCGGGA TCGCCTCCAC 
CTGCCGCAGT CGAACATCGA TACTCGAGGC ACGCAGCAGA TGCTGGACTT CTTTGCGCAG 
TGCATCGAGG TCAAGGGACC GTTGCTGGTG GACCAGTTGT TCCACCTGCT GACCACCAAT 
TTCCCGCAGG ACCAGTGGCT GCGCATGTTC AAGACGCCGG GCGACCTGAG CTCATTCCTC 
AAGCTCTTCG CCGACTGCTT CCACATACAG GCCAATCTAG TCACCCTGCT GCAGAAGCCC 
AAGCTCAGCG ACACGCACAT TCAGCAGGCA CAGGCTCAAA CCCGGGAGCA ATTTAATGCG 
CTGAACAATA ACAACAGTGC CAGCATTCGA AAGCAGGAGC CAACACCTGG AGGTGGAGGC 
GTTGGAGGTG TCAGCTCGGT GCAGCAGAGA CTGCAGTCGC CGGCACTGCG GACTAATGGT 
CACACGAACA ACAACAATGG GAGCAACGGC AGCAACAATA ACAACAACAA CAACAACAGC 
ATTGCCTGCC CAAACTTCAA GCTAAATGCG CCCGTTTCGA ATGTGATGGG TGGACAGAGC 
CAGGGGTTCG GGCAGCCCAA GTCGGAGCCC AGCTCTGGCT TTGACAGCTA CGTGCCCATG 
TCGGAGCTGA AGCTGGAGAA TCTGTGCGAG AACAACTATC CCAGTGCGAA CACCTGCTAC 
GGGCCCATTA ATAACTCCAG CCAGCAGACG CAGCAAGTGC AAACGCAGCA GCAGCAGCAG 
CCGCAGCATG CGACACAGAA TCCGGCGGAG CAGCGCTTGA ATAGTGTTAA CCAAACGCTG 
AAGCAACGCA TCAACACCCT GGTTATACGG ACTCTGGCCG AGAACCTGGA GAAGGACAAG 
CAATCGCTGG CCAACCAGCA GGGCGGGCCC ATTTCCCCGC ACGCCAGTCC CGTGCATTCC 
ATAGCCAATT CGAGTTCGAA CCAAAATGCC GGTAGTGCTG CAAATAACGC CAATAGCAAC 
TCGAATGCGA ATCCGAACAA TGCCAACCAC TCACCTAGTC ATAGTTACTT TGTCGGCGAC 
ACCTGGAAGA TAAAGGTGCT GCAGAACACC ACGGTGATAG CGAATGTCAA GCAGTCGGTG 
TTTGTGACCG ACATCATACT GAAGTATGCG GCCAAGAACG AGAGCATAGT GGTCTCCCTG 
GACTGCGAGG GCATTAACCT GGGCCTGAAG GGCGAGATCA CCCTGATTGA GATTGGCACA 
ACGCGGGGCG AAGCCTTCTT GTTCGACGTG CAGTCCTGCC CGGCGATGGT AACCGATGGC 
GGATTGAAGA CCGTGTTGGA GCACGACCAG GTGATCAAGG TTATACACGA CTGCCGCAAC 
GACGCTGCCA ACCTTTATCT GCAATTCGGC ATCCTGCTGC GGAATGTGTT CGACACCCAG 
GCCGCACATG CCATCTTGCA GTATCAGGAG AGCGGCAAGC AGGTCTACAA GGCCAAGTAC 
ATATCGTTAA ACTCGCTGTG CGAGCAGTAC AACGCTCCCT GCAACCCGAT CAAGGATCAG 
TTGAAGCAGA TCTACCGCAG GGACCAGAAG TTCTGGGCGA AGAGACCCCT CACACGCGAA 
ATGATGCTGT ACGCAGCGGG CGATGTCCTG GTGCTCATCC ACGATCAGCT ATTCGGCAAC 
CTGGCGCGGC AGATCAAGCC AGAGAATCGG GCTCTCTTCT CAGAGCTGTG CACCGAGCAA 
ATACTCATGC AGATCAAACC CAACGAGGTC AAGATACGCA AGAAGCAGCG CAAGGTCAGC 
ACCGAGGTGT CCGATCTCAA ACAGAAGCTG GCCCAGACCA GCAAGAGCAT TGTGCTCTCC 
AATCGCGAGA TACGCTTGCT GCGGTATATG GATCTGACAG AGGACGAGAA AGAGCGCCTC 
AAGGGCTACT ACAAGGTGGC GAAGAAGCTG GAGAAGATGG AGTCCGCCGG CAATCCCAGC 
AAAGATCAAA GTGACTCCGA GGATGAACAA GAGCCGAACG AGAACGACGC CTTTCCCAGT 
TTGGATTCGG TGCCCTCGGA CAACTCGCTT TCGGGCACAT TTTCGCCACG CTTCAGTTCA 
GAGCCACCCA GCCTGACTGA ATCCATGCAA ATGCTGGAAG AGATTCTCCA GAACAAGTCA 
ATGGATCGCA TAGCCCGTAT TGACAAGCTG GAGGCCATTC TGACGACTGC CACCTCGCTG 
CCATGTGAAC AAATTATAGC TTCTAATTCT ATGCAAGAGC AATTAGGTTC GAGCATTGCG 
ACCACCGAGA ATCTACAGAT TATACGCGAG AAATCCAAAA ACATTAAGAA CTGCAATTGC 
CAGGGCGAGC GGAGTGTGAC GCCCATTATG CGAACGACTG ACAAGCGCGT GGTGAAGCTC 
GTGGACGCCG AATCGCAGAC ACTGAGCACC GGCGACGTGG TCATCACCAA GATATTCTTC 
CAGGACGAGC ACGAGCGAGC CAAGGAGGCG GCCCTGCTGA GCAACTCGCC CGCGAAGCGT 
GTGTCTCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286779.1
CDS	200..3214
Translation	MESMEYEMARNMTLLFFLERLLDKGEPRTVHDLSCQFGNKEFTKEMRQIAGGSQSGLKKFLAQYPAIFLVDGDYVQVNAYQHHNADDGGCGGKRDYIQEAKDYFKNKMLQYGAAAEVPVRSLLGHRSQASPQVRHISGQHIKEFTDFLMKHTDTFKVTDDYVMLVGCENMTDLPARDRLHLPQSNIDTRGTQQMLDFFAQCIEVKGPLLVDQLFHLLTTNFPQDQWLRMFKTPGDLSSFLKLFADCFHIQANLVTLLQKPKLSDTHIQQAQAQTREQFNALNNNNSASIRKQEPTPGGGGVGGVSSVQQRLQSPALRTNGHTNNNNGSNGSNNNNNNNNSIACPNFKLNAPVSNVMGGQSQGFGQPKSEPSSGFDSYVPMSELKLENLCENNYPSANTCYGPINNSSQQTQQVQTQQQQQPQHATQNPAEQRLNSVNQTLKQRINTLVIRTLAENLEKDKQSLANQQGGPISPHASPVHSIANSSSNQNAGSAANNANSNSNANPNNANHSPSHSYFVGDTWKIKVLQNTTVIANVKQSVFVTDIILKYAAKNESIVVSLDCEGINLGLKGEITLIEIGTTRGEAFLFDVQSCPAMVTDGGLKTVLEHDQVIKVIHDCRNDAANLYLQFGILLRNVFDTQAAHAILQYQESGKQVYKAKYISLNSLCEQYNAPCNPIKDQLKQIYRRDQKFWAKRPLTREMMLYAAGDVLVLIHDQLFGNLARQIKPENRALFSELCTEQILMQIKPNEVKIRKKQRKVSTEVSDLKQKLAQTSKSIVLSNREIRLLRYMDLTEDEKERLKGYYKVAKKLEKMESAGNPSKDQSDSEDEQEPNENDAFPSLDSVPSDNSLSGTFSPRFSSEPPSLTESMQMLEEILQNKSMDRIARIDKLEAILTTATSLPCEQIIASNSMQEQLGSSIATTENLQIIREKSKNIKNCNCQGERSVTPIMRTTDKRVVKLVDAESQTLSTGDVVITKIFFQDEHERAKEAALLSNSPAKRVSPT

Target ORF information:

RefSeq Version	NM_001299850.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster egalitarian (egl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299850.1

ORF Insert Sequence:

ATGGAGTCCA TGGAGTACGA GATGGCACGC AACATGACGT TGCTCTTCTT CCTGGAGCGA 
CTGCTGGACA AGGGCGAGCC CCGCACCGTC CACGACCTGT CCTGCCAATT CGGCAACAAG 
GAGTTCACCA AGGAGATGCG CCAAATCGCC GGTGGCAGTC AGTCGGGTCT GAAGAAGTTC 
CTCGCCCAGT ACCCGGCCAT ATTTCTGGTT GACGGCGACT ACGTCCAGGT GAACGCCTAT 
CAGCACCACA ACGCGGACGA TGGCGGCTGC GGGGGCAAGC GGGACTACAT CCAAGAGGCT 
AAAGACTACT TCAAGAACAA GATGCTGCAG TACGGAGCGG CTGCCGAGGT GCCCGTCCGC 
AGTCTCCTGG GCCACCGGTC GCAGGCGTCG CCCCAAGTGC GTCACATATC GGGCCAGCAC 
ATCAAGGAGT TCACCGACTT CCTGATGAAG CACACGGACA CCTTCAAGGT GACGGACGAC 
TATGTGATGC TGGTGGGCTG CGAGAACATG ACGGATCTGC CGGCGCGGGA TCGCCTCCAC 
CTGCCGCAGT CGAACATCGA TACTCGAGGC ACGCAGCAGA TGCTGGACTT CTTTGCGCAG 
TGCATCGAGG TCAAGGGACC GTTGCTGGTG GACCAGTTGT TCCACCTGCT GACCACCAAT 
TTCCCGCAGG ACCAGTGGCT GCGCATGTTC AAGACGCCGG GCGACCTGAG CTCATTCCTC 
AAGCTCTTCG CCGACTGCTT CCACATACAG GCCAATCTAG TCACCCTGCT GCAGAAGCCC 
AAGCTCAGCG ACACGCACAT TCAGCAGGCA CAGGCTCAAA CCCGGGAGCA ATTTAATGCG 
CTGAACAATA ACAACAGTGC CAGCATTCGA AAGCAGGAGC CAACACCTGG AGGTGGAGGC 
GTTGGAGGTG TCAGCTCGGT GCAGCAGAGA CTGCAGTCGC CGGCACTGCG GACTAATGGT 
CACACGAACA ACAACAATGG GAGCAACGGC AGCAACAATA ACAACAACAA CAACAACAGC 
ATTGCCTGCC CAAACTTCAA GCTAAATGCG CCCGTTTCGA ATGTGATGGG TGGACAGAGC 
CAGGGGTTCG GGCAGCCCAA GTCGGAGCCC AGCTCTGGCT TTGACAGCTA CGTGCCCATG 
TCGGAGCTGA AGCTGGAGAA TCTGTGCGAG AACAACTATC CCAGTGCGAA CACCTGCTAC 
GGGCCCATTA ATAACTCCAG CCAGCAGACG CAGCAAGTGC AAACGCAGCA GCAGCAGCAG 
CCGCAGCATG CGACACAGAA TCCGGCGGAG CAGCGCTTGA ATAGTGTTAA CCAAACGCTG 
AAGCAACGCA TCAACACCCT GGTTATACGG ACTCTGGCCG AGAACCTGGA GAAGGACAAG 
CAATCGCTGG CCAACCAGCA GGGCGGGCCC ATTTCCCCGC ACGCCAGTCC CGTGCATTCC 
ATAGCCAATT CGAGTTCGAA CCAAAATGCC GGTAGTGCTG CAAATAACGC CAATAGCAAC 
TCGAATGCGA ATCCGAACAA TGCCAACCAC TCACCTAGTC ATAGTTACTT TGTCGGCGAC 
ACCTGGAAGA TAAAGGTGCT GCAGAACACC ACGGTGATAG CGAATGTCAA GCAGTCGGTG 
TTTGTGACCG ACATCATACT GAAGTATGCG GCCAAGAACG AGAGCATAGT GGTCTCCCTG 
GACTGCGAGG GCATTAACCT GGGCCTGAAG GGCGAGATCA CCCTGATTGA GATTGGCACA 
ACGCGGGGCG AAGCCTTCTT GTTCGACGTG CAGTCCTGCC CGGCGATGGT AACCGATGGC 
GGATTGAAGA CCGTGTTGGA GCACGACCAG GTGATCAAGG TTATACACGA CTGCCGCAAC 
GACGCTGCCA ACCTTTATCT GCAATTCGGC ATCCTGCTGC GGAATGTGTT CGACACCCAG 
GCCGCACATG CCATCTTGCA GTATCAGGAG AGCGGCAAGC AGGTCTACAA GGCCAAGTAC 
ATATCGTTAA ACTCGCTGTG CGAGCAGTAC AACGCTCCCT GCAACCCGAT CAAGGATCAG 
TTGAAGCAGA TCTACCGCAG GGACCAGAAG TTCTGGGCGA AGAGACCCCT CACACGCGAA 
ATGATGCTGT ACGCAGCGGG CGATGTCCTG GTGCTCATCC ACGATCAGCT ATTCGGCAAC 
CTGGCGCGGC AGATCAAGCC AGAGAATCGG GCTCTCTTCT CAGAGCTGTG CACCGAGCAA 
ATACTCATGC AGATCAAACC CAACGAGGTC AAGATACGCA AGAAGCAGCG CAAGGTCAGC 
ACCGAGGTGT CCGATCTCAA ACAGAAGCTG GCCCAGACCA GCAAGAGCAT TGTGCTCTCC 
AATCGCGAGA TACGCTTGCT GCGGTATATG GATCTGACAG AGGACGAGAA AGAGCGCCTC 
AAGGGCTACT ACAAGGTGGC GAAGAAGCTG GAGAAGATGG AGTCCGCCGG CAATCCCAGC 
AAAGATCAAA GTGACTCCGA GGATGAACAA GAGCCGAACG AGAACGACGC CTTTCCCAGT 
TTGGATTCGG TGCCCTCGGA CAACTCGCTT TCGGGCACAT TTTCGCCACG CTTCAGTTCA 
GAGCCACCCA GCCTGACTGA ATCCATGCAA ATGCTGGAAG AGATTCTCCA GAACAAGTCA 
ATGGATCGCA TAGCCCGTAT TGACAAGCTG GAGGCCATTC TGACGACTGC CACCTCGCTG 
CCATGTGAAC AAATTATAGC TTCTAATTCT ATGCAAGAGC AATTAGGTTC GAGCATTGCG 
ACCACCGAGA ATCTACAGAT TATACGCGAG AAATCCAAAA ACATTAAGAA CTGCAATTGC 
CAGGGCGAGC GGAGTGTGAC GCCCATTATG CGAACGACTG ACAAGCGCGT GGTGAAGCTC 
GTGGACGCCG AATCGCAGAC ACTGAGCACC GGCGACGTGG TCATCACCAA GATATTCTTC 
CAGGACGAGC ACGAGCGAGC CAAGGAGGCG GCCCTGCTGA GCAACTCGCC CGCGAAGCGT 
GTGTCTCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egl ( NM_166623.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726360.3 Drosophila melanogaster egalitarian (egl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13171
Clone ID Related Accession (Same CDS sequence)	NM_001299850.1 , NM_166623.3
Accession Version	NM_166623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	egalitarian, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166623.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCCA TGGAGTACGA GATGGCACGC AACATGACGT TGCTCTTCTT CCTGGAGCGA 
CTGCTGGACA AGGGCGAGCC CCGCACCGTC CACGACCTGT CCTGCCAATT CGGCAACAAG 
GAGTTCACCA AGGAGATGCG CCAAATCGCC GGTGGCAGTC AGTCGGGTCT GAAGAAGTTC 
CTCGCCCAGT ACCCGGCCAT ATTTCTGGTT GACGGCGACT ACGTCCAGGT GAACGCCTAT 
CAGCACCACA ACGCGGACGA TGGCGGCTGC GGGGGCAAGC GGGACTACAT CCAAGAGGCT 
AAAGACTACT TCAAGAACAA GATGCTGCAG TACGGAGCGG CTGCCGAGGT GCCCGTCCGC 
AGTCTCCTGG GCCACCGGTC GCAGGCGTCG CCCCAAGTGC GTCACATATC GGGCCAGCAC 
ATCAAGGAGT TCACCGACTT CCTGATGAAG CACACGGACA CCTTCAAGGT GACGGACGAC 
TATGTGATGC TGGTGGGCTG CGAGAACATG ACGGATCTGC CGGCGCGGGA TCGCCTCCAC 
CTGCCGCAGT CGAACATCGA TACTCGAGGC ACGCAGCAGA TGCTGGACTT CTTTGCGCAG 
TGCATCGAGG TCAAGGGACC GTTGCTGGTG GACCAGTTGT TCCACCTGCT GACCACCAAT 
TTCCCGCAGG ACCAGTGGCT GCGCATGTTC AAGACGCCGG GCGACCTGAG CTCATTCCTC 
AAGCTCTTCG CCGACTGCTT CCACATACAG GCCAATCTAG TCACCCTGCT GCAGAAGCCC 
AAGCTCAGCG ACACGCACAT TCAGCAGGCA CAGGCTCAAA CCCGGGAGCA ATTTAATGCG 
CTGAACAATA ACAACAGTGC CAGCATTCGA AAGCAGGAGC CAACACCTGG AGGTGGAGGC 
GTTGGAGGTG TCAGCTCGGT GCAGCAGAGA CTGCAGTCGC CGGCACTGCG GACTAATGGT 
CACACGAACA ACAACAATGG GAGCAACGGC AGCAACAATA ACAACAACAA CAACAACAGC 
ATTGCCTGCC CAAACTTCAA GCTAAATGCG CCCGTTTCGA ATGTGATGGG TGGACAGAGC 
CAGGGGTTCG GGCAGCCCAA GTCGGAGCCC AGCTCTGGCT TTGACAGCTA CGTGCCCATG 
TCGGAGCTGA AGCTGGAGAA TCTGTGCGAG AACAACTATC CCAGTGCGAA CACCTGCTAC 
GGGCCCATTA ATAACTCCAG CCAGCAGACG CAGCAAGTGC AAACGCAGCA GCAGCAGCAG 
CCGCAGCATG CGACACAGAA TCCGGCGGAG CAGCGCTTGA ATAGTGTTAA CCAAACGCTG 
AAGCAACGCA TCAACACCCT GGTTATACGG ACTCTGGCCG AGAACCTGGA GAAGGACAAG 
CAATCGCTGG CCAACCAGCA GGGCGGGCCC ATTTCCCCGC ACGCCAGTCC CGTGCATTCC 
ATAGCCAATT CGAGTTCGAA CCAAAATGCC GGTAGTGCTG CAAATAACGC CAATAGCAAC 
TCGAATGCGA ATCCGAACAA TGCCAACCAC TCACCTAGTC ATAGTTACTT TGTCGGCGAC 
ACCTGGAAGA TAAAGGTGCT GCAGAACACC ACGGTGATAG CGAATGTCAA GCAGTCGGTG 
TTTGTGACCG ACATCATACT GAAGTATGCG GCCAAGAACG AGAGCATAGT GGTCTCCCTG 
GACTGCGAGG GCATTAACCT GGGCCTGAAG GGCGAGATCA CCCTGATTGA GATTGGCACA 
ACGCGGGGCG AAGCCTTCTT GTTCGACGTG CAGTCCTGCC CGGCGATGGT AACCGATGGC 
GGATTGAAGA CCGTGTTGGA GCACGACCAG GTGATCAAGG TTATACACGA CTGCCGCAAC 
GACGCTGCCA ACCTTTATCT GCAATTCGGC ATCCTGCTGC GGAATGTGTT CGACACCCAG 
GCCGCACATG CCATCTTGCA GTATCAGGAG AGCGGCAAGC AGGTCTACAA GGCCAAGTAC 
ATATCGTTAA ACTCGCTGTG CGAGCAGTAC AACGCTCCCT GCAACCCGAT CAAGGATCAG 
TTGAAGCAGA TCTACCGCAG GGACCAGAAG TTCTGGGCGA AGAGACCCCT CACACGCGAA 
ATGATGCTGT ACGCAGCGGG CGATGTCCTG GTGCTCATCC ACGATCAGCT ATTCGGCAAC 
CTGGCGCGGC AGATCAAGCC AGAGAATCGG GCTCTCTTCT CAGAGCTGTG CACCGAGCAA 
ATACTCATGC AGATCAAACC CAACGAGGTC AAGATACGCA AGAAGCAGCG CAAGGTCAGC 
ACCGAGGTGT CCGATCTCAA ACAGAAGCTG GCCCAGACCA GCAAGAGCAT TGTGCTCTCC 
AATCGCGAGA TACGCTTGCT GCGGTATATG GATCTGACAG AGGACGAGAA AGAGCGCCTC 
AAGGGCTACT ACAAGGTGGC GAAGAAGCTG GAGAAGATGG AGTCCGCCGG CAATCCCAGC 
AAAGATCAAA GTGACTCCGA GGATGAACAA GAGCCGAACG AGAACGACGC CTTTCCCAGT 
TTGGATTCGG TGCCCTCGGA CAACTCGCTT TCGGGCACAT TTTCGCCACG CTTCAGTTCA 
GAGCCACCCA GCCTGACTGA ATCCATGCAA ATGCTGGAAG AGATTCTCCA GAACAAGTCA 
ATGGATCGCA TAGCCCGTAT TGACAAGCTG GAGGCCATTC TGACGACTGC CACCTCGCTG 
CCATGTGAAC AAATTATAGC TTCTAATTCT ATGCAAGAGC AATTAGGTTC GAGCATTGCG 
ACCACCGAGA ATCTACAGAT TATACGCGAG AAATCCAAAA ACATTAAGAA CTGCAATTGC 
CAGGGCGAGC GGAGTGTGAC GCCCATTATG CGAACGACTG ACAAGCGCGT GGTGAAGCTC 
GTGGACGCCG AATCGCAGAC ACTGAGCACC GGCGACGTGG TCATCACCAA GATATTCTTC 
CAGGACGAGC ACGAGCGAGC CAAGGAGGCG GCCCTGCTGA GCAACTCGCC CGCGAAGCGT 
GTGTCTCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726360.3
CDS	743..3757
Translation	MESMEYEMARNMTLLFFLERLLDKGEPRTVHDLSCQFGNKEFTKEMRQIAGGSQSGLKKFLAQYPAIFLVDGDYVQVNAYQHHNADDGGCGGKRDYIQEAKDYFKNKMLQYGAAAEVPVRSLLGHRSQASPQVRHISGQHIKEFTDFLMKHTDTFKVTDDYVMLVGCENMTDLPARDRLHLPQSNIDTRGTQQMLDFFAQCIEVKGPLLVDQLFHLLTTNFPQDQWLRMFKTPGDLSSFLKLFADCFHIQANLVTLLQKPKLSDTHIQQAQAQTREQFNALNNNNSASIRKQEPTPGGGGVGGVSSVQQRLQSPALRTNGHTNNNNGSNGSNNNNNNNNSIACPNFKLNAPVSNVMGGQSQGFGQPKSEPSSGFDSYVPMSELKLENLCENNYPSANTCYGPINNSSQQTQQVQTQQQQQPQHATQNPAEQRLNSVNQTLKQRINTLVIRTLAENLEKDKQSLANQQGGPISPHASPVHSIANSSSNQNAGSAANNANSNSNANPNNANHSPSHSYFVGDTWKIKVLQNTTVIANVKQSVFVTDIILKYAAKNESIVVSLDCEGINLGLKGEITLIEIGTTRGEAFLFDVQSCPAMVTDGGLKTVLEHDQVIKVIHDCRNDAANLYLQFGILLRNVFDTQAAHAILQYQESGKQVYKAKYISLNSLCEQYNAPCNPIKDQLKQIYRRDQKFWAKRPLTREMMLYAAGDVLVLIHDQLFGNLARQIKPENRALFSELCTEQILMQIKPNEVKIRKKQRKVSTEVSDLKQKLAQTSKSIVLSNREIRLLRYMDLTEDEKERLKGYYKVAKKLEKMESAGNPSKDQSDSEDEQEPNENDAFPSLDSVPSDNSLSGTFSPRFSSEPPSLTESMQMLEEILQNKSMDRIARIDKLEAILTTATSLPCEQIIASNSMQEQLGSSIATTENLQIIREKSKNIKNCNCQGERSVTPIMRTTDKRVVKLVDAESQTLSTGDVVITKIFFQDEHERAKEAALLSNSPAKRVSPT

Target ORF information:

RefSeq Version	NM_166623.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster egalitarian (egl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166623.3

ORF Insert Sequence:

ATGGAGTCCA TGGAGTACGA GATGGCACGC AACATGACGT TGCTCTTCTT CCTGGAGCGA 
CTGCTGGACA AGGGCGAGCC CCGCACCGTC CACGACCTGT CCTGCCAATT CGGCAACAAG 
GAGTTCACCA AGGAGATGCG CCAAATCGCC GGTGGCAGTC AGTCGGGTCT GAAGAAGTTC 
CTCGCCCAGT ACCCGGCCAT ATTTCTGGTT GACGGCGACT ACGTCCAGGT GAACGCCTAT 
CAGCACCACA ACGCGGACGA TGGCGGCTGC GGGGGCAAGC GGGACTACAT CCAAGAGGCT 
AAAGACTACT TCAAGAACAA GATGCTGCAG TACGGAGCGG CTGCCGAGGT GCCCGTCCGC 
AGTCTCCTGG GCCACCGGTC GCAGGCGTCG CCCCAAGTGC GTCACATATC GGGCCAGCAC 
ATCAAGGAGT TCACCGACTT CCTGATGAAG CACACGGACA CCTTCAAGGT GACGGACGAC 
TATGTGATGC TGGTGGGCTG CGAGAACATG ACGGATCTGC CGGCGCGGGA TCGCCTCCAC 
CTGCCGCAGT CGAACATCGA TACTCGAGGC ACGCAGCAGA TGCTGGACTT CTTTGCGCAG 
TGCATCGAGG TCAAGGGACC GTTGCTGGTG GACCAGTTGT TCCACCTGCT GACCACCAAT 
TTCCCGCAGG ACCAGTGGCT GCGCATGTTC AAGACGCCGG GCGACCTGAG CTCATTCCTC 
AAGCTCTTCG CCGACTGCTT CCACATACAG GCCAATCTAG TCACCCTGCT GCAGAAGCCC 
AAGCTCAGCG ACACGCACAT TCAGCAGGCA CAGGCTCAAA CCCGGGAGCA ATTTAATGCG 
CTGAACAATA ACAACAGTGC CAGCATTCGA AAGCAGGAGC CAACACCTGG AGGTGGAGGC 
GTTGGAGGTG TCAGCTCGGT GCAGCAGAGA CTGCAGTCGC CGGCACTGCG GACTAATGGT 
CACACGAACA ACAACAATGG GAGCAACGGC AGCAACAATA ACAACAACAA CAACAACAGC 
ATTGCCTGCC CAAACTTCAA GCTAAATGCG CCCGTTTCGA ATGTGATGGG TGGACAGAGC 
CAGGGGTTCG GGCAGCCCAA GTCGGAGCCC AGCTCTGGCT TTGACAGCTA CGTGCCCATG 
TCGGAGCTGA AGCTGGAGAA TCTGTGCGAG AACAACTATC CCAGTGCGAA CACCTGCTAC 
GGGCCCATTA ATAACTCCAG CCAGCAGACG CAGCAAGTGC AAACGCAGCA GCAGCAGCAG 
CCGCAGCATG CGACACAGAA TCCGGCGGAG CAGCGCTTGA ATAGTGTTAA CCAAACGCTG 
AAGCAACGCA TCAACACCCT GGTTATACGG ACTCTGGCCG AGAACCTGGA GAAGGACAAG 
CAATCGCTGG CCAACCAGCA GGGCGGGCCC ATTTCCCCGC ACGCCAGTCC CGTGCATTCC 
ATAGCCAATT CGAGTTCGAA CCAAAATGCC GGTAGTGCTG CAAATAACGC CAATAGCAAC 
TCGAATGCGA ATCCGAACAA TGCCAACCAC TCACCTAGTC ATAGTTACTT TGTCGGCGAC 
ACCTGGAAGA TAAAGGTGCT GCAGAACACC ACGGTGATAG CGAATGTCAA GCAGTCGGTG 
TTTGTGACCG ACATCATACT GAAGTATGCG GCCAAGAACG AGAGCATAGT GGTCTCCCTG 
GACTGCGAGG GCATTAACCT GGGCCTGAAG GGCGAGATCA CCCTGATTGA GATTGGCACA 
ACGCGGGGCG AAGCCTTCTT GTTCGACGTG CAGTCCTGCC CGGCGATGGT AACCGATGGC 
GGATTGAAGA CCGTGTTGGA GCACGACCAG GTGATCAAGG TTATACACGA CTGCCGCAAC 
GACGCTGCCA ACCTTTATCT GCAATTCGGC ATCCTGCTGC GGAATGTGTT CGACACCCAG 
GCCGCACATG CCATCTTGCA GTATCAGGAG AGCGGCAAGC AGGTCTACAA GGCCAAGTAC 
ATATCGTTAA ACTCGCTGTG CGAGCAGTAC AACGCTCCCT GCAACCCGAT CAAGGATCAG 
TTGAAGCAGA TCTACCGCAG GGACCAGAAG TTCTGGGCGA AGAGACCCCT CACACGCGAA 
ATGATGCTGT ACGCAGCGGG CGATGTCCTG GTGCTCATCC ACGATCAGCT ATTCGGCAAC 
CTGGCGCGGC AGATCAAGCC AGAGAATCGG GCTCTCTTCT CAGAGCTGTG CACCGAGCAA 
ATACTCATGC AGATCAAACC CAACGAGGTC AAGATACGCA AGAAGCAGCG CAAGGTCAGC 
ACCGAGGTGT CCGATCTCAA ACAGAAGCTG GCCCAGACCA GCAAGAGCAT TGTGCTCTCC 
AATCGCGAGA TACGCTTGCT GCGGTATATG GATCTGACAG AGGACGAGAA AGAGCGCCTC 
AAGGGCTACT ACAAGGTGGC GAAGAAGCTG GAGAAGATGG AGTCCGCCGG CAATCCCAGC 
AAAGATCAAA GTGACTCCGA GGATGAACAA GAGCCGAACG AGAACGACGC CTTTCCCAGT 
TTGGATTCGG TGCCCTCGGA CAACTCGCTT TCGGGCACAT TTTCGCCACG CTTCAGTTCA 
GAGCCACCCA GCCTGACTGA ATCCATGCAA ATGCTGGAAG AGATTCTCCA GAACAAGTCA 
ATGGATCGCA TAGCCCGTAT TGACAAGCTG GAGGCCATTC TGACGACTGC CACCTCGCTG 
CCATGTGAAC AAATTATAGC TTCTAATTCT ATGCAAGAGC AATTAGGTTC GAGCATTGCG 
ACCACCGAGA ATCTACAGAT TATACGCGAG AAATCCAAAA ACATTAAGAA CTGCAATTGC 
CAGGGCGAGC GGAGTGTGAC GCCCATTATG CGAACGACTG ACAAGCGCGT GGTGAAGCTC 
GTGGACGCCG AATCGCAGAC ACTGAGCACC GGCGACGTGG TCATCACCAA GATATTCTTC 
CAGGACGAGC ACGAGCGAGC CAAGGAGGCG GCCCTGCTGA GCAACTCGCC CGCGAAGCGT 
GTGTCTCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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egl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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