twi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	twi
Entrez Gene ID	37655
Full Name	twist
Synonyms	CG2956,DTwist,Dmel\CG2956,EC9,TWI,Tw,Twi,Twist,bHLHb17,dip5
General protein information	Preferred Names twist Names CG2956 gene product from transcript CG2956-RC CG2956-PA CG2956-PB CG2956-PC twi-PA twi-PB twi-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59C1-59C2\|2-100 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299823.1	NP_001286752.1	twist, isoform C
NM_079092.3	NP_523816.2	twist, isoform A
NM_001038878.2	NP_001033967.1	twist, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	twist3	NP_571060.2
Drosophila melanogaster (fruit fly)	twi	NP_001033967.1
Xenopus tropicalis (tropical clawed frog)	twist2	NP_001096679.1
Gallus gallus (chicken)	TWIST3	NP_001096684.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings provide mechanistic insight into the brake on myogenesis that occurs during mesoderm specification: twist and tin expression at early stages in turn activate the myogenic inhibitor Him; yet, once Twist or Tin levels decline at mid-embryogenesis, Him is no longer expressed in the mesoderm, and MEF2-dependent muscle differentiation can proceed.
Title: The myogenic repressor gene Holes in muscles is a direct transcriptional target of Twist and Tinman in the Drosophila embryonic mesoderm.

The level of Twi activity determines whether the cellular events of ventral furrow formation, epithelial-to-mesenchymal transition, cell division and mesodermal migration occur.
Title: Discrete levels of Twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis.

Our studies identify Akirin as a nuclear factor that genetically interacts with the BRM complex and is required for optimal expression of the Twist-dependent Dmef2 enhancer
Title: Akirin links twist-regulated transcription with the Brahma chromatin remodeling complex during embryogenesis.

conclude that Twi is an essential Notch co-regulator in myogenic progenitor cells and has the potential to confer specificity on Notch signalling at over 170 genes, showing that a single factor can have a profound effect on the output of the pathway.
Title: Specificity of Notch pathway activation: twist controls the transcriptional output in adult muscle progenitors.

Data show that cell fate decisions in the dorsal and lateral Twi- and Tin-positive mesoderm of Drosophila melanogaster depend on the antagonistic action of the transcription factor Lame duck and the zinc finger homeodomain factor Zfh1.
Title: Antagonistic function of Lmd and Zfh1 fine tunes cell fate decisions in the Twi and Tin positive mesoderm of Drosophila melanogaster.

The following twi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12622	NM_079092.3 Latest version!	Drosophila melanogaster twist (twi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa12622	NM_001299823.1 Latest version!	Drosophila melanogaster twist (twi), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa12622	NM_001038878.2 Latest version!	Drosophila melanogaster twist (twi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twi ( NM_001299823.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286752.1 Drosophila melanogaster twist (twi), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12622
Clone ID Related Accession (Same CDS sequence)	NM_001299823.1 , NM_079092.3 , NM_001038878.2
Accession Version	NM_001299823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twist, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286752.1
CDS	647..2119
Translation	MMSARSVSPKVLLDISYKPTLPNIMELQNNVIKLIQVEQQAYMQSGYQLQHQQQHLHSHQHHQQHHQQQHAQYAPLPSEYAAYGITELEDTDYNIPSNEVLSTSSNQSAQSTSLELNNNNTSSNTNSSGNNPSGFDGQASSGSSWNEHGKRARSSGDYDCQTGGSLVMQPEHKKLIHQQQQQQQQHQQQIYVDYLPTTVDEVASAQSCPGVQSTCTSPQSHFDFPDEELPEHKAQVFLPLYNNQQQQSQQLQQQQPHQQSHAQMHFQNAYRQSFEGYEPANSLNGSAYSSSDRDDMEYARHNALSSVSDLNGGVMSPACLADDGSAGSLLDGSDAGGKAFRKPRRRLKRKPSKTEETDEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSPSAYGSASSLLSAAANGAEADLKCLRKANGAPIIPPEKLSYLFGVWRMEGDAQHQKA

Target ORF information:

RefSeq Version	NM_001299823.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twist (twi), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299823.1

ORF Insert Sequence:

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twi ( NM_079092.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523816.2 Drosophila melanogaster twist (twi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12622
Clone ID Related Accession (Same CDS sequence)	NM_001299823.1 , NM_079092.3 , NM_001038878.2
Accession Version	NM_079092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twist, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523816.2
CDS	376..1848
Translation	MMSARSVSPKVLLDISYKPTLPNIMELQNNVIKLIQVEQQAYMQSGYQLQHQQQHLHSHQHHQQHHQQQHAQYAPLPSEYAAYGITELEDTDYNIPSNEVLSTSSNQSAQSTSLELNNNNTSSNTNSSGNNPSGFDGQASSGSSWNEHGKRARSSGDYDCQTGGSLVMQPEHKKLIHQQQQQQQQHQQQIYVDYLPTTVDEVASAQSCPGVQSTCTSPQSHFDFPDEELPEHKAQVFLPLYNNQQQQSQQLQQQQPHQQSHAQMHFQNAYRQSFEGYEPANSLNGSAYSSSDRDDMEYARHNALSSVSDLNGGVMSPACLADDGSAGSLLDGSDAGGKAFRKPRRRLKRKPSKTEETDEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSPSAYGSASSLLSAAANGAEADLKCLRKANGAPIIPPEKLSYLFGVWRMEGDAQHQKA

Target ORF information:

RefSeq Version	NM_079092.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twist (twi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079092.3

ORF Insert Sequence:

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twi ( NM_001038878.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033967.1 Drosophila melanogaster twist (twi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12622
Clone ID Related Accession (Same CDS sequence)	NM_001299823.1 , NM_079092.3 , NM_001038878.2
Accession Version	NM_001038878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twist, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001038878.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033967.1
CDS	162..1634
Translation	MMSARSVSPKVLLDISYKPTLPNIMELQNNVIKLIQVEQQAYMQSGYQLQHQQQHLHSHQHHQQHHQQQHAQYAPLPSEYAAYGITELEDTDYNIPSNEVLSTSSNQSAQSTSLELNNNNTSSNTNSSGNNPSGFDGQASSGSSWNEHGKRARSSGDYDCQTGGSLVMQPEHKKLIHQQQQQQQQHQQQIYVDYLPTTVDEVASAQSCPGVQSTCTSPQSHFDFPDEELPEHKAQVFLPLYNNQQQQSQQLQQQQPHQQSHAQMHFQNAYRQSFEGYEPANSLNGSAYSSSDRDDMEYARHNALSSVSDLNGGVMSPACLADDGSAGSLLDGSDAGGKAFRKPRRRLKRKPSKTEETDEFSNQRVMANVRERQRTQSLNDAFKSLQQIIPTLPSDKLSKIQTLKLATRYIDFLCRMLSSSDISLLKALEAQGSPSAYGSASSLLSAAANGAEADLKCLRKANGAPIIPPEKLSYLFGVWRMEGDAQHQKA

Target ORF information:

RefSeq Version	NM_001038878.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twist (twi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038878.2

ORF Insert Sequence:

ATGATGAGCG CTCGCTCGGT GTCGCCCAAA GTGCTGCTGG ACATAAGCTA CAAGCCCACA 
CTGCCCAACA TCATGGAGCT GCAGAACAAT GTGATCAAGC TGATACAGGT GGAGCAGCAG 
GCCTACATGC AGTCCGGCTA TCAGCTGCAG CACCAGCAGC AGCACCTCCA CTCCCACCAG 
CACCACCAGC AGCACCACCA GCAGCAGCAT GCCCAGTACG CCCCACTGCC CTCGGAGTAC 
GCCGCCTATG GTATTACCGA ACTGGAGGAC ACAGACTACA ACATACCCAG CAACGAGGTC 
CTGAGCACCA GCAGCAACCA GAGTGCCCAG AGCACCAGTC TGGAGCTGAA CAACAACAAC 
ACCAGCAGCA ACACCAACAG CTCCGGCAAC AATCCGAGTG GCTTCGATGG CCAGGCCAGC 
AGCGGATCCT CTTGGAACGA GCACGGCAAG AGGGCCAGGA GCAGTGGCGA CTACGATTGC 
CAAACCGGGG GATCACTGGT CATGCAGCCC GAGCACAAGA AGCTAATCCA CCAGCAACAG 
CAGCAGCAAC AACAGCACCA GCAACAGATC TATGTGGATT ACTTGCCCAC CACCGTGGAC 
GAGGTGGCCT CGGCTCAATC TTGTCCTGGC GTCCAGAGCA CGTGCACCTC CCCGCAATCC 
CACTTCGATT TTCCCGACGA GGAGCTGCCC GAGCACAAGG CCCAGGTGTT CCTGCCCCTC 
TACAACAACC AGCAGCAGCA GTCGCAGCAG CTGCAACAGC AGCAGCCGCA CCAGCAAAGC 
CACGCCCAGA TGCACTTCCA AAACGCCTAC AGACAAAGTT TCGAGGGCTA CGAGCCGGCC 
AACTCGCTGA ACGGCAGTGC TTACTCCAGC TCGGATCGGG ATGACATGGA GTACGCCCGC 
CACAACGCCC TGAGTTCAGT TAGCGATCTC AACGGAGGAG TCATGTCGCC CGCCTGCTTG 
GCGGATGACG GCAGTGCCGG CAGTTTGCTG GACGGATCCG ATGCCGGCGG AAAGGCCTTC 
CGCAAGCCAC GTCGCCGGCT GAAGCGGAAG CCCAGCAAGA CGGAGGAGAC GGACGAGTTC 
AGCAACCAGC GGGTCATGGC CAATGTGAGG GAGCGCCAGC GCACCCAGAG CCTCAACGAC 
GCCTTCAAGT CCCTGCAGCA GATCATCCCC ACGCTGCCCA GCGACAAGCT CAGCAAGATC 
CAGACCCTCA AACTGGCCAC AAGATACATT GACTTCCTGT GCCGCATGCT CAGCTCGAGT 
GATATATCTT TGCTGAAGGC CTTGGAGGCC CAGGGATCGC CCTCGGCGTA TGGATCGGCC 
AGCTCCCTCC TGAGTGCCGC CGCCAATGGA GCCGAGGCAG ATCTGAAGTG CCTGCGCAAG 
GCCAACGGAG CACCCATTAT CCCGCCCGAG AAGCTGAGTT ATCTGTTCGG GGTGTGGCGC 
ATGGAGGGCG ACGCGCAGCA CCAGAAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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twi cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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