dve cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dve
Entrez Gene ID	37546
Full Name	defective proventriculus
Synonyms	CG5799,DVE,Dmel\CG5799,Dve,Dve-s,SATB1,dep,l(2)01738
General protein information	Preferred Names defective proventriculus Names CG5799 gene product from transcript CG5799-RA CG5799-PA CG5799-PB compass dve-PA dve-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58D2-58D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057894.4	NP_477242.1	defective proventriculus, isoform A
NM_057893.5	NP_477241.2	defective proventriculus, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006642	XP_316670.4
Drosophila melanogaster (fruit fly)	dve	NP_477241.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function of dve regulatory motifs in cell differentiation.
Title: Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation.

Ectopic antenna formation in heads with dve mutant clones was associated with ectopic Dll expression and endogenous Hth expression in the vertex region.
Title: A vertex specific dorsal selector Dve represses the ventral appendage identity in Drosophila head.

Male accessory gland secondary cells are crucial for male fecundity, and Dve activity is required for their survival.
Title: The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila.

The homeobox gene defective proventriculus (dve) is expressed in the midgut, and two transcripts, type A and type B, have been identified
Title: Spatial and temporal requirement of defective proventriculus activity during Drosophila midgut development.

Dve suppresses the potential joint-forming activity, and that Notch signaling represses Dve expression to form joints.
Title: Notch signaling relieves the joint-suppressive activity of Defective proventriculus in the Drosophila leg.

The following dve gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dve cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10909	NM_057893.5 Latest version!	Drosophila melanogaster defective proventriculus (dve), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10908	NM_057894.4 Latest version!	Drosophila melanogaster defective proventriculus (dve), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dve ( NM_057893.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477241.2 Drosophila melanogaster defective proventriculus (dve), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10909
Clone ID Related Accession (Same CDS sequence)	NM_057893.5
Accession Version	NM_057893.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective proventriculus, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057893.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGAAG AAGCCAAATC ACTTCCACTT CACTGTGTGG TGGAGTCGGT GCACTCACTG 
CACGCCTCCC TGACCATCGA CACTCGCCAG CCCTGGAAGA GGCGGCCCAA TATCGAGACC 
GACAGCTACG TGATCATCGC CGCGGCCACG CCCTGGAGCG AGATTGTTCA GACGGCGCTC 
CAGAGGCTCG GCTACTCCCA GGAGGTGGCC AACACAGCCA GAGGCTCATT AATAATCAAA 
CATTGGAAAC CCATACCCCT GGAGCAGATA TCGGACAATC CAGCGGTGCC CGTCAGCGAT 
ATTGTGGGTG AATTGACTTC CGTGATCACG CTGAGGATCG TGATTCTGCG CCCCAAGACT 
TCGCCGTTCG GGGAGATCAA GGACAAGCTG CTCAAGCTGC TGGTGCTTCA GTCGCATGCG 
GTGCTCCGTT CCACAGGATG TCCGCTGGAT GAGGTGACGC TATCCCAGAT CTGCCGGAGT 
TCCCACCAGA ACACCTACGC CCTGCCAGGA GGAGAGATCT CCGACGAACT GCGCCGCAAG 
TTCGACCAGT GGTGGTCCAA TCAGCTCTCT CCCCAAGCGA CCGCCATGAC GCCCAAGATG 
CTGCCGTTCA TGACGGGTCC TTCAGCGGTT CCGGTTCCAG TGGCAGTGCC CGGCGAGATG 
GACTTTCCGG TAGGCACGGC CATGGCGGCG GCGGCCGCTG CGGCGGCTCA TGCTGCCGCT 
GCCGGTGGAG CAGCGGGCGG CAATCCCCTG GGATCGATGG GTAGTCGCGA GAGTCTGTTG 
CTGGCTAACG AGGCTGCTCA CCATGCGGGC GCTGGACAGG CTCCGGGACC AGGACATCAC 
GGCAGCATGC TGGTGCATCC GATGCACGCG TCCATGCACC ACCATCACCA TCATGGCGGC 
GGCGGTGGAC ACGGTCCTCC GTATCCCAAT CAGAAGACTC GCATGCGCAC CAGCTTCGAT 
CCGGAGATGG AGCTGCCCAA GCTGCAGAAG TGGTTCGCCG ACAATCCGCA TCCTTCGCGA 
CAGCAGATCC AGACGTATGT GGTGCAGCTG AATGCCCTGG AGTCTCGCCG GGGAAGGAAG 
CCCCTGGATG TGAACAACGT GGTCTACTGG TTCAAGAACG CTCGGGCGGC TCAAAAACGG 
GCAGAGATGC GGGGTGGTAG TCTGGGCAGT GCGATCAGTG CCCTGGGTCA CGCCGCCATG 
AACGGCTATC TGAGTCAGCA CGCCCCTTTG GGCCAGAATT CCAGCAGCAG TGCTGGTAGC 
CAGCCCATGA GCATGGGCAA CCTGTCCATG TCGCACGACT ACCTAAAGAG TCCGCTCAGC 
CTGAAGTCGG AGGACATTGA CACCATGTCG CAGCACTCGG ACGACATGGA CGAGGAGCAG 
AGCAGACCGA ACACGCCACA ACTGCCCCTT TCGCTGACCA CCCACGAGCG GCATCGCAGC 
TCACCGCTGA TGGACGAGGA CGAGGATGAG GCGGGAGAGC AGCAGCAGCC ACAGGAGGCC 
AAAAGCGATG GAATGAACGG TAGTGTGAAG GATGAGGAGC GGCAGGAGAA GTCGCGCGAT 
GAACTGCAGG ACAACGAAAA GGAGAACTCC AACGGGGAAG ACCGTCATCT GGATGCAGAC 
GCCCAGATGG AGGCCAGTTC CAACCTCAAC AATAACCACA ACAACAGCAG CTTGCACATT 
GAGCAGGAGG TGGGCTCCAC ACCCAAGCGC TCCACGCCCA AGGAGGAGGA CGACGACTTG 
GATATGGACG AGGATGAGGA GGACAACGAA AACGATGCCA GCCATTTGGA CGAGTTTCGC 
TCGCCCTCGC CGGATCTAGC AGGTGGAGTT GTGCCCCACA AGGACCAGCT GCCCTTTCCT 
ATGGTTCCCA ACTCCATGTT CTCGCAGTCC TTCATGTACA TGAGCCACTA CATCCCGGCA 
TTCGGTCAAG CGGCCGCCGG ACATCCGCAT CACCATGCTG CCGCTGCAGC GGCAGCTGCC 
GGAATCCAAC CCAACGCCCT CATGGGCGGC GGCGGCCTCA ACCTGTCGAG CATATCGAAC 
GAGGAGCGCC GGAAGCGAAA CCGCACCTTC ATCGACCCGG TGACCGAGGT GCCCAAGCTG 
GAGCAGTGGT TCGCCATGAA CACACATCCC TCGCACAACC TCATCCTCAA GTACACCGAG 
GACTTGAACA CCATGCCATA TAGGCAAAAG TTCCCGCGCT TGGAGAGCAA AAACGTGCAG 
TTCTGGTTCA AGAATCGACG GGCCAAGTGC AAGCGCCTCA AGATGTCGCT GTACGACAGC 
AATCAGTGCG CCCAGCTGGG AGGACTCGGC TCTTTTGTGC CCAAGTACGA GGAGCGGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477241.2
CDS	18..2360
Translation	MLEEAKSLPLHCVVESVHSLHASLTIDTRQPWKRRPNIETDSYVIIAAATPWSEIVQTALQRLGYSQEVANTARGSLIIKHWKPIPLEQISDNPAVPVSDIVGELTSVITLRIVILRPKTSPFGEIKDKLLKLLVLQSHAVLRSTGCPLDEVTLSQICRSSHQNTYALPGGEISDELRRKFDQWWSNQLSPQATAMTPKMLPFMTGPSAVPVPVAVPGEMDFPVGTAMAAAAAAAAHAAAAGGAAGGNPLGSMGSRESLLLANEAAHHAGAGQAPGPGHHGSMLVHPMHASMHHHHHHGGGGGHGPPYPNQKTRMRTSFDPEMELPKLQKWFADNPHPSRQQIQTYVVQLNALESRRGRKPLDVNNVVYWFKNARAAQKRAEMRGGSLGSAISALGHAAMNGYLSQHAPLGQNSSSSAGSQPMSMGNLSMSHDYLKSPLSLKSEDIDTMSQHSDDMDEEQSRPNTPQLPLSLTTHERHRSSPLMDEDEDEAGEQQQPQEAKSDGMNGSVKDEERQEKSRDELQDNEKENSNGEDRHLDADAQMEASSNLNNNHNNSSLHIEQEVGSTPKRSTPKEEDDDLDMDEDEEDNENDASHLDEFRSPSPDLAGGVVPHKDQLPFPMVPNSMFSQSFMYMSHYIPAFGQAAAGHPHHHAAAAAAAAGIQPNALMGGGGLNLSSISNEERRKRNRTFIDPVTEVPKLEQWFAMNTHPSHNLILKYTEDLNTMPYRQKFPRLESKNVQFWFKNRRAKCKRLKMSLYDSNQCAQLGGLGSFVPKYEERD

Target ORF information:

RefSeq Version	NM_057893.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective proventriculus (dve), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057893.5

ORF Insert Sequence:

ATGCTGGAAG AAGCCAAATC ACTTCCACTT CACTGTGTGG TGGAGTCGGT GCACTCACTG 
CACGCCTCCC TGACCATCGA CACTCGCCAG CCCTGGAAGA GGCGGCCCAA TATCGAGACC 
GACAGCTACG TGATCATCGC CGCGGCCACG CCCTGGAGCG AGATTGTTCA GACGGCGCTC 
CAGAGGCTCG GCTACTCCCA GGAGGTGGCC AACACAGCCA GAGGCTCATT AATAATCAAA 
CATTGGAAAC CCATACCCCT GGAGCAGATA TCGGACAATC CAGCGGTGCC CGTCAGCGAT 
ATTGTGGGTG AATTGACTTC CGTGATCACG CTGAGGATCG TGATTCTGCG CCCCAAGACT 
TCGCCGTTCG GGGAGATCAA GGACAAGCTG CTCAAGCTGC TGGTGCTTCA GTCGCATGCG 
GTGCTCCGTT CCACAGGATG TCCGCTGGAT GAGGTGACGC TATCCCAGAT CTGCCGGAGT 
TCCCACCAGA ACACCTACGC CCTGCCAGGA GGAGAGATCT CCGACGAACT GCGCCGCAAG 
TTCGACCAGT GGTGGTCCAA TCAGCTCTCT CCCCAAGCGA CCGCCATGAC GCCCAAGATG 
CTGCCGTTCA TGACGGGTCC TTCAGCGGTT CCGGTTCCAG TGGCAGTGCC CGGCGAGATG 
GACTTTCCGG TAGGCACGGC CATGGCGGCG GCGGCCGCTG CGGCGGCTCA TGCTGCCGCT 
GCCGGTGGAG CAGCGGGCGG CAATCCCCTG GGATCGATGG GTAGTCGCGA GAGTCTGTTG 
CTGGCTAACG AGGCTGCTCA CCATGCGGGC GCTGGACAGG CTCCGGGACC AGGACATCAC 
GGCAGCATGC TGGTGCATCC GATGCACGCG TCCATGCACC ACCATCACCA TCATGGCGGC 
GGCGGTGGAC ACGGTCCTCC GTATCCCAAT CAGAAGACTC GCATGCGCAC CAGCTTCGAT 
CCGGAGATGG AGCTGCCCAA GCTGCAGAAG TGGTTCGCCG ACAATCCGCA TCCTTCGCGA 
CAGCAGATCC AGACGTATGT GGTGCAGCTG AATGCCCTGG AGTCTCGCCG GGGAAGGAAG 
CCCCTGGATG TGAACAACGT GGTCTACTGG TTCAAGAACG CTCGGGCGGC TCAAAAACGG 
GCAGAGATGC GGGGTGGTAG TCTGGGCAGT GCGATCAGTG CCCTGGGTCA CGCCGCCATG 
AACGGCTATC TGAGTCAGCA CGCCCCTTTG GGCCAGAATT CCAGCAGCAG TGCTGGTAGC 
CAGCCCATGA GCATGGGCAA CCTGTCCATG TCGCACGACT ACCTAAAGAG TCCGCTCAGC 
CTGAAGTCGG AGGACATTGA CACCATGTCG CAGCACTCGG ACGACATGGA CGAGGAGCAG 
AGCAGACCGA ACACGCCACA ACTGCCCCTT TCGCTGACCA CCCACGAGCG GCATCGCAGC 
TCACCGCTGA TGGACGAGGA CGAGGATGAG GCGGGAGAGC AGCAGCAGCC ACAGGAGGCC 
AAAAGCGATG GAATGAACGG TAGTGTGAAG GATGAGGAGC GGCAGGAGAA GTCGCGCGAT 
GAACTGCAGG ACAACGAAAA GGAGAACTCC AACGGGGAAG ACCGTCATCT GGATGCAGAC 
GCCCAGATGG AGGCCAGTTC CAACCTCAAC AATAACCACA ACAACAGCAG CTTGCACATT 
GAGCAGGAGG TGGGCTCCAC ACCCAAGCGC TCCACGCCCA AGGAGGAGGA CGACGACTTG 
GATATGGACG AGGATGAGGA GGACAACGAA AACGATGCCA GCCATTTGGA CGAGTTTCGC 
TCGCCCTCGC CGGATCTAGC AGGTGGAGTT GTGCCCCACA AGGACCAGCT GCCCTTTCCT 
ATGGTTCCCA ACTCCATGTT CTCGCAGTCC TTCATGTACA TGAGCCACTA CATCCCGGCA 
TTCGGTCAAG CGGCCGCCGG ACATCCGCAT CACCATGCTG CCGCTGCAGC GGCAGCTGCC 
GGAATCCAAC CCAACGCCCT CATGGGCGGC GGCGGCCTCA ACCTGTCGAG CATATCGAAC 
GAGGAGCGCC GGAAGCGAAA CCGCACCTTC ATCGACCCGG TGACCGAGGT GCCCAAGCTG 
GAGCAGTGGT TCGCCATGAA CACACATCCC TCGCACAACC TCATCCTCAA GTACACCGAG 
GACTTGAACA CCATGCCATA TAGGCAAAAG TTCCCGCGCT TGGAGAGCAA AAACGTGCAG 
TTCTGGTTCA AGAATCGACG GGCCAAGTGC AAGCGCCTCA AGATGTCGCT GTACGACAGC 
AATCAGTGCG CCCAGCTGGG AGGACTCGGC TCTTTTGTGC CCAAGTACGA GGAGCGGGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dve ( NM_057894.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477242.1 Drosophila melanogaster defective proventriculus (dve), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10908
Clone ID Related Accession (Same CDS sequence)	NM_057894.4
Accession Version	NM_057894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective proventriculus, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_057894.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTTCC AGAGCGCCAT GGAAACCTTT GCCGAAGCCT GGGTGGCAGC CAATGCCGGT 
CAGCAGAGTC AGGCCTTGGC CTTAACCAGA GCTGTCAACG CGGCCGCCGT TGTGGCCTCG 
GCCAATGCCA AATCCCCCAA TGCCTCCTCC TCCGCCTCGG CCAGCCTGGC GGATCTGGCC 
GTCAAGAAGG AGCACTCCAT CTCGCCGCCC CACAGTGTGG TGAGCGTGGG TGTGGGCGTG 
GCTGGCGAGG ATCAGCATCG CAGATCCTCG TTGCAGGGTG TGCAGGAGAA GCTCACCCAG 
CTGCAGATGC AGCAGCAGCA TCACCAGCAG CAGCAGCAAC AGCAGCAGCA GCAACAGCAA 
CAGCACCAGC AGCAGCAGCA ACATCATCAT CATCAGCAGC AAGCACAGCA GGCAGGCGCT 
GGAGTGGGTA GTGGCGAGCT GGCTGGACGC AGATCCTCCG AGGGCACACC ACCCACCGCC 
CATCCCCCAA CGAACTCCGA GGGACAGGCG CTCTCCTCCT CCACAGCCTC ATCTAGTCAG 
AATGCCACCC CCAAGAGCGA TCGGGAAAGG GAGCGTGAGC GGGAGAGGGA TCGGGAGGAG 
CGCGTGGAGC GGGGCAGCAT ATCCTGCCTC CCACCAGCCG CCCTTGGAAT GGCCGTGGGC 
GTCCAGCAGG CCCAGCATCA GGAGAAGCTG CTCAGCCATC CGGCCTTGGA TTCGCGACGG 
GAGTTCGACG TCAGCAAAGT GAATCCCAAA TCACTTCCAC TTCACTGTGT GGTGGAGTCG 
GTGCACTCAC TGCACGCCTC CCTGACCATC GACACTCGCC AGCCCTGGAA GAGGCGGCCC 
AATATCGAGA CCGACAGCTA CGTGATCATC GCCGCGGCCA CGCCCTGGAG CGAGATTGTT 
CAGACGGCGC TCCAGAGGCT CGGCTACTCC CAGGAGGTGG CCAACACAGC CAGAGGCTCA 
TTAATAATCA AACATTGGAA ACCCATACCC CTGGAGCAGA TATCGGACAA TCCAGCGGTG 
CCCGTCAGCG ATATTGTGGG TGAATTGACT TCCGTGATCA CGCTGAGGAT CGTGATTCTG 
CGCCCCAAGA CTTCGCCGTT CGGGGAGATC AAGGACAAGC TGCTCAAGCT GCTGGTGCTT 
CAGTCGCATG CGGTGCTCCG TTCCACAGGA TGTCCGCTGG ATGAGGTGAC GCTATCCCAG 
ATCTGCCGGA GTTCCCACCA GAACACCTAC GCCCTGCCAG GAGGAGAGAT CTCCGACGAA 
CTGCGCCGCA AGTTCGACCA GTGGTGGTCC AATCAGCTCT CTCCCCAAGC GACCGCCATG 
ACGCCCAAGA TGCTGCCGTT CATGACGGGT CCTTCAGCGG TTCCGGTTCC AGTGGCAGTG 
CCCGGCGAGA TGGACTTTCC GGTAGGCACG GCCATGGCGG CGGCGGCCGC TGCGGCGGCT 
CATGCTGCCG CTGCCGGTGG AGCAGCGGGC GGCAATCCCC TGGGATCGAT GGGTAGTCGC 
GAGAGTCTGT TGCTGGCTAA CGAGGCTGCT CACCATGCGG GCGCTGGACA GGCTCCGGGA 
CCAGGACATC ACGGCAGCAT GCTGGTGCAT CCGATGCACG CGTCCATGCA CCACCATCAC 
CATCATGGCG GCGGCGGTGG ACACGGTCCT CCGTATCCCA ATCAGAAGAC TCGCATGCGC 
ACCAGCTTCG ATCCGGAGAT GGAGCTGCCC AAGCTGCAGA AGTGGTTCGC CGACAATCCG 
CATCCTTCGC GACAGCAGAT CCAGACGTAT GTGGTGCAGC TGAATGCCCT GGAGTCTCGC 
CGGGGAAGGA AGCCCCTGGA TGTGAACAAC GTGGTCTACT GGTTCAAGAA CGCTCGGGCG 
GCTCAAAAAC GGGCAGAGAT GCGGGGTGGT AGTCTGGGCA GTGCGATCAG TGCCCTGGGT 
CACGCCGCCA TGAACGGCTA TCTGAGTCAG CACGCCCCTT TGGGCCAGAA TTCCAGCAGC 
AGTGCTGGTA GCCAGCCCAT GAGCATGGGC AACCTGTCCA TGTCGCACGA CTACCTAAAG 
AGTCCGCTCA GCCTGAAGTC GGAGGACATT GACACCATGT CGCAGCACTC GGACGACATG 
GACGAGGAGC AGAGCAGACC GAACACGCCA CAACTGCCCC TTTCGCTGAC CACCCACGAG 
CGGCATCGCA GCTCACCGCT GATGGACGAG GACGAGGATG AGGCGGGAGA GCAGCAGCAG 
CCACAGGAGG CCAAAAGCGA TGGAATGAAC GGTAGTGTGA AGGATGAGGA GCGGCAGGAG 
AAGTCGCGCG ATGAACTGCA GGACAACGAA AAGGAGAACT CCAACGGGGA AGACCGTCAT 
CTGGATGCAG ACGCCCAGAT GGAGGCCAGT TCCAACCTCA ACAATAACCA CAACAACAGC 
AGCTTGCACA TTGAGCAGGA GGTGGGCTCC ACACCCAAGC GCTCCACGCC CAAGGAGGAG 
GACGACGACT TGGATATGGA CGAGGATGAG GAGGACAACG AAAACGATGC CAGCCATTTG 
GACGAGTTTC GCTCGCCCTC GCCGGATCTA GCAGGTGGAG TTGTGCCCCA CAAGGACCAG 
CTGCCCTTTC CTATGGTTCC CAACTCCATG TTCTCGCAGT CCTTCATGTA CATGAGCCAC 
TACATCCCGG CATTCGGTCA AGCGGCCGCC GGACATCCGC ATCACCATGC TGCCGCTGCA 
GCGGCAGCTG CCGGAATCCA ACCCAACGCC CTCATGGGCG GCGGCGGCCT CAACCTGTCG 
AGCATATCGA ACGAGGAGCG CCGGAAGCGA AACCGCACCT TCATCGACCC GGTGACCGAG 
GTGCCCAAGC TGGAGCAGTG GTTCGCCATG AACACACATC CCTCGCACAA CCTCATCCTC 
AAGTACACCG AGGACTTGAA CACCATGCCA TATAGGCAAA AGTTCCCGCG CTTGGAGAGC 
AAAAACGTGC AGTTCTGGTT CAAGAATCGA CGGGCCAAGT GCAAGCGCCT CAAGATGTCG 
CTGTACGACA GCAATCAGTG CGCCCAGCTG GGAGGACTCG GCTCTTTTGT GCCCAAGTAC 
GAGGAGCGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477242.1
CDS	720..3794
Translation	MDFQSAMETFAEAWVAANAGQQSQALALTRAVNAAAVVASANAKSPNASSSASASLADLAVKKEHSISPPHSVVSVGVGVAGEDQHRRSSLQGVQEKLTQLQMQQQHHQQQQQQQQQQQQQHQQQQQHHHHQQQAQQAGAGVGSGELAGRRSSEGTPPTAHPPTNSEGQALSSSTASSSQNATPKSDRERERERERDREERVERGSISCLPPAALGMAVGVQQAQHQEKLLSHPALDSRREFDVSKVNPKSLPLHCVVESVHSLHASLTIDTRQPWKRRPNIETDSYVIIAAATPWSEIVQTALQRLGYSQEVANTARGSLIIKHWKPIPLEQISDNPAVPVSDIVGELTSVITLRIVILRPKTSPFGEIKDKLLKLLVLQSHAVLRSTGCPLDEVTLSQICRSSHQNTYALPGGEISDELRRKFDQWWSNQLSPQATAMTPKMLPFMTGPSAVPVPVAVPGEMDFPVGTAMAAAAAAAAHAAAAGGAAGGNPLGSMGSRESLLLANEAAHHAGAGQAPGPGHHGSMLVHPMHASMHHHHHHGGGGGHGPPYPNQKTRMRTSFDPEMELPKLQKWFADNPHPSRQQIQTYVVQLNALESRRGRKPLDVNNVVYWFKNARAAQKRAEMRGGSLGSAISALGHAAMNGYLSQHAPLGQNSSSSAGSQPMSMGNLSMSHDYLKSPLSLKSEDIDTMSQHSDDMDEEQSRPNTPQLPLSLTTHERHRSSPLMDEDEDEAGEQQQPQEAKSDGMNGSVKDEERQEKSRDELQDNEKENSNGEDRHLDADAQMEASSNLNNNHNNSSLHIEQEVGSTPKRSTPKEEDDDLDMDEDEEDNENDASHLDEFRSPSPDLAGGVVPHKDQLPFPMVPNSMFSQSFMYMSHYIPAFGQAAAGHPHHHAAAAAAAAGIQPNALMGGGGLNLSSISNEERRKRNRTFIDPVTEVPKLEQWFAMNTHPSHNLILKYTEDLNTMPYRQKFPRLESKNVQFWFKNRRAKCKRLKMSLYDSNQCAQLGGLGSFVPKYEERD

Target ORF information:

RefSeq Version	NM_057894.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective proventriculus (dve), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057894.4

ORF Insert Sequence:

ATGGACTTCC AGAGCGCCAT GGAAACCTTT GCCGAAGCCT GGGTGGCAGC CAATGCCGGT 
CAGCAGAGTC AGGCCTTGGC CTTAACCAGA GCTGTCAACG CGGCCGCCGT TGTGGCCTCG 
GCCAATGCCA AATCCCCCAA TGCCTCCTCC TCCGCCTCGG CCAGCCTGGC GGATCTGGCC 
GTCAAGAAGG AGCACTCCAT CTCGCCGCCC CACAGTGTGG TGAGCGTGGG TGTGGGCGTG 
GCTGGCGAGG ATCAGCATCG CAGATCCTCG TTGCAGGGTG TGCAGGAGAA GCTCACCCAG 
CTGCAGATGC AGCAGCAGCA TCACCAGCAG CAGCAGCAAC AGCAGCAGCA GCAACAGCAA 
CAGCACCAGC AGCAGCAGCA ACATCATCAT CATCAGCAGC AAGCACAGCA GGCAGGCGCT 
GGAGTGGGTA GTGGCGAGCT GGCTGGACGC AGATCCTCCG AGGGCACACC ACCCACCGCC 
CATCCCCCAA CGAACTCCGA GGGACAGGCG CTCTCCTCCT CCACAGCCTC ATCTAGTCAG 
AATGCCACCC CCAAGAGCGA TCGGGAAAGG GAGCGTGAGC GGGAGAGGGA TCGGGAGGAG 
CGCGTGGAGC GGGGCAGCAT ATCCTGCCTC CCACCAGCCG CCCTTGGAAT GGCCGTGGGC 
GTCCAGCAGG CCCAGCATCA GGAGAAGCTG CTCAGCCATC CGGCCTTGGA TTCGCGACGG 
GAGTTCGACG TCAGCAAAGT GAATCCCAAA TCACTTCCAC TTCACTGTGT GGTGGAGTCG 
GTGCACTCAC TGCACGCCTC CCTGACCATC GACACTCGCC AGCCCTGGAA GAGGCGGCCC 
AATATCGAGA CCGACAGCTA CGTGATCATC GCCGCGGCCA CGCCCTGGAG CGAGATTGTT 
CAGACGGCGC TCCAGAGGCT CGGCTACTCC CAGGAGGTGG CCAACACAGC CAGAGGCTCA 
TTAATAATCA AACATTGGAA ACCCATACCC CTGGAGCAGA TATCGGACAA TCCAGCGGTG 
CCCGTCAGCG ATATTGTGGG TGAATTGACT TCCGTGATCA CGCTGAGGAT CGTGATTCTG 
CGCCCCAAGA CTTCGCCGTT CGGGGAGATC AAGGACAAGC TGCTCAAGCT GCTGGTGCTT 
CAGTCGCATG CGGTGCTCCG TTCCACAGGA TGTCCGCTGG ATGAGGTGAC GCTATCCCAG 
ATCTGCCGGA GTTCCCACCA GAACACCTAC GCCCTGCCAG GAGGAGAGAT CTCCGACGAA 
CTGCGCCGCA AGTTCGACCA GTGGTGGTCC AATCAGCTCT CTCCCCAAGC GACCGCCATG 
ACGCCCAAGA TGCTGCCGTT CATGACGGGT CCTTCAGCGG TTCCGGTTCC AGTGGCAGTG 
CCCGGCGAGA TGGACTTTCC GGTAGGCACG GCCATGGCGG CGGCGGCCGC TGCGGCGGCT 
CATGCTGCCG CTGCCGGTGG AGCAGCGGGC GGCAATCCCC TGGGATCGAT GGGTAGTCGC 
GAGAGTCTGT TGCTGGCTAA CGAGGCTGCT CACCATGCGG GCGCTGGACA GGCTCCGGGA 
CCAGGACATC ACGGCAGCAT GCTGGTGCAT CCGATGCACG CGTCCATGCA CCACCATCAC 
CATCATGGCG GCGGCGGTGG ACACGGTCCT CCGTATCCCA ATCAGAAGAC TCGCATGCGC 
ACCAGCTTCG ATCCGGAGAT GGAGCTGCCC AAGCTGCAGA AGTGGTTCGC CGACAATCCG 
CATCCTTCGC GACAGCAGAT CCAGACGTAT GTGGTGCAGC TGAATGCCCT GGAGTCTCGC 
CGGGGAAGGA AGCCCCTGGA TGTGAACAAC GTGGTCTACT GGTTCAAGAA CGCTCGGGCG 
GCTCAAAAAC GGGCAGAGAT GCGGGGTGGT AGTCTGGGCA GTGCGATCAG TGCCCTGGGT 
CACGCCGCCA TGAACGGCTA TCTGAGTCAG CACGCCCCTT TGGGCCAGAA TTCCAGCAGC 
AGTGCTGGTA GCCAGCCCAT GAGCATGGGC AACCTGTCCA TGTCGCACGA CTACCTAAAG 
AGTCCGCTCA GCCTGAAGTC GGAGGACATT GACACCATGT CGCAGCACTC GGACGACATG 
GACGAGGAGC AGAGCAGACC GAACACGCCA CAACTGCCCC TTTCGCTGAC CACCCACGAG 
CGGCATCGCA GCTCACCGCT GATGGACGAG GACGAGGATG AGGCGGGAGA GCAGCAGCAG 
CCACAGGAGG CCAAAAGCGA TGGAATGAAC GGTAGTGTGA AGGATGAGGA GCGGCAGGAG 
AAGTCGCGCG ATGAACTGCA GGACAACGAA AAGGAGAACT CCAACGGGGA AGACCGTCAT 
CTGGATGCAG ACGCCCAGAT GGAGGCCAGT TCCAACCTCA ACAATAACCA CAACAACAGC 
AGCTTGCACA TTGAGCAGGA GGTGGGCTCC ACACCCAAGC GCTCCACGCC CAAGGAGGAG 
GACGACGACT TGGATATGGA CGAGGATGAG GAGGACAACG AAAACGATGC CAGCCATTTG 
GACGAGTTTC GCTCGCCCTC GCCGGATCTA GCAGGTGGAG TTGTGCCCCA CAAGGACCAG 
CTGCCCTTTC CTATGGTTCC CAACTCCATG TTCTCGCAGT CCTTCATGTA CATGAGCCAC 
TACATCCCGG CATTCGGTCA AGCGGCCGCC GGACATCCGC ATCACCATGC TGCCGCTGCA 
GCGGCAGCTG CCGGAATCCA ACCCAACGCC CTCATGGGCG GCGGCGGCCT CAACCTGTCG 
AGCATATCGA ACGAGGAGCG CCGGAAGCGA AACCGCACCT TCATCGACCC GGTGACCGAG 
GTGCCCAAGC TGGAGCAGTG GTTCGCCATG AACACACATC CCTCGCACAA CCTCATCCTC 
AAGTACACCG AGGACTTGAA CACCATGCCA TATAGGCAAA AGTTCCCGCG CTTGGAGAGC 
AAAAACGTGC AGTTCTGGTT CAAGAATCGA CGGGCCAAGT GCAAGCGCCT CAAGATGTCG 
CTGTACGACA GCAATCAGTG CGCCCAGCTG GGAGGACTCG GCTCTTTTGT GCCCAAGTAC 
GAGGAGCGGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dve cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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