Topors cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Topors
Entrez Gene ID	37188
Full Name	Topoisomerase I-interacting protein
Synonyms	CG15104,Dmel\CG15104,TOPORS,dTopors,dtopors,nbl
General protein information	Preferred Names Topoisomerase I-interacting protein Names CG15104 gene product from transcript CG15104-RB CG15104-PA CG15104-PB Topors-PA Topors-PB nuclear blebber topoisomerase I-interacting protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56A2-56A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_137544.3	NP_611388.1	topoisomerase I-interacting protein, isoform A
NM_001274154.1	NP_001261083.1	topoisomerase I-interacting protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dtopors plays a structural role in spermatocyte lamina that is critical for multiple aspects of meiotic chromosome transmission
Title: Nuclear structure and chromosome segregation in Drosophila male meiosis depend on the ubiquitin ligase dTopors.

Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein
Title: Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein.

The following Topors gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Topors cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10561	NM_137544.3 Latest version!	Drosophila melanogaster topoisomerase I-interacting protein, transcript variant A (Topors), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10561	NM_001274154.1 Latest version!	Drosophila melanogaster topoisomerase I-interacting protein, transcript variant B (Topors), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Topors ( NM_137544.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611388.1 Drosophila melanogaster topoisomerase I-interacting protein, transcript variant A (Topors), mRNA.

Price & Availability↑

CloneID	OFa10561
Clone ID Related Accession (Same CDS sequence)	NM_137544.3 , NM_001274154.1
Accession Version	NM_137544.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase I-interacting protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137544.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGG AGAATCCCGG AGCTCTTGCC GCCAACGTGC CCTATTTGGG AGTGGATGAG 
TTGGGCGCAT CGGTGATTGT GGAGCCTGGT TTGGAAGGAT CCAATGCCGG AGGCCGGACG 
CTACCGGCTG CGGCCATTAA GTTTGCTGAT CTCACCGAGA GCGGCAGTGA GTCGGGAGAC 
AACGAGGCCG AGGAACCTGT ATCCGCTGGC CCGGATAATG CCAACGCCAT CGGTGAGCCG 
GGCACCAGTG CATCCGCCGC GGAGGAGAAC GGAACAGTAG AGCGCAACTC GCCGCCGCCC 
AATTGCGCCA TCTGCTTGTC CCGCTGCAGG AGGAAGTGCT TCACGGACTC GTGCATGCAC 
CAGTTCTGCT TCAAGTGCCT GTGCGAGTGG AGCAAGATAA AGCCCGAGTG TCCACTGTGT 
AAGCAGCCCT TCAGAACCAT CATACACAAT GTCCGCACCC TGGACGACTA CGATCGCTAC 
CCGGTGCAGA CTACGTCGCC GGTTCCAACA GAAAATCCCT CCCTACGTTA CCACATTGTG 
AGGCGTCCCA GGTACACGCC GCTGGTGCAG AACCAGGCCG TCATTGTCAA CGACATCGAG 
GCGGCCATAG CGGCAGGAGC TGCTGGTGAA GATGTGCTTT CGGCGGCAGA AGTAGCAGCT 
GGCAGGCGAT CGTACAGCCG CTTTGAGCCG TATCGCTCGG AGCTGATGAA TTACTACCAG 
CACGATCAGG ATGCATCCAC GTCGGGTTCA TTGAGCCAGC TCTGGCGACG TTATGTGTAC 
GATAGAAAGC TGTATGCTCT GCCCGTCAGC GACAGCGTGA CTGGACACTT TCGAGAGTGG 
AGCGCCCGTT TTTACAGAAA CAATCCTGCT CAGATTCATC GCCTGATGCC TTGGATCCAT 
CGCGACATCA TGTGCCTACT GAGGAATGCC GCACATAGCG TAAATACTGT GATGACTCTG 
ATGAGCGACC TCCTGCCCAT GACTAGCTTA CTAGGTCCTA CATTCCGTCG ACGGCTGTCG 
CCGTATCTCG GCGAGCGCAC CAGTCACTTT ATCCACGAAC TGTTTAACTT TGCCCGCTCC 
CCGTATGACA TAAACGGTTA CGATCATGTG GTGCAGTATT CGGCACGAGT GGCTGAGGAG 
GTGGAGGTGG ATCTGTTGGA CATGGTGGAG ACACAATCCT CGAACGGTGA TGATCTGAAT 
TTGGAGGTCG GAGATAGCGA TGCGGATGCT ATAAACGCTG GCTTCTCTCC GGATTGGAGC 
CCCCCTAGGG TACGCCCGTC GACCAGTGTG ATTGTGACCA ATCCTGGTGC CACACATTCC 
TTCAGCGTGA CAATGGCCAG TGATGGCAGT GAGCTGCCGG GGATTTCCAT ACGCCGTACA 
ACCAATGTGG GCTCACAAAC GGTTGCCATT AACCTGAGCA TGAGGCGCCC AGCGGCGGTT 
GCCAGCGAGG AACCAGAGGT CATCGAGATC GACGACGGCG ATGCGGCCGC AAATGCAGAG 
GTGGCGGCCA TCAATGATGG CAGCAACACC AGTAGGAGAC ACGCAGGTGC TACTCTTCCA 
GTGACGGCGC ACATCGAACT GGAGAGCAGC AGCAGTTCCG GCGATGAGGA TGAGTGCGTC 
TTTGTGCTGG AGCTGAAGCC ACCACACATG CGCACTCCCG AACAGGTGAG CCTTGACTCA 
AATAGTGACT CCGATGTGGT GTTCGTAAAT GAGCAGCACG AAGCTGCGCC GGACGCAATT 
GCCGAAAACC GGTCGACACA ATCCCCTTTG GATCTGGCCT CCAGGGATCA GGGCCTGTTT 
ATGGGGCCCA GCACAAGTGG CGCAGCGGCC AACAGAGGCA AGAACTGGAA GCTGGTCATG 
GCGCAGACAC GGCGCCTCGA TCAGTTGCGC ACGCTGCGCT CTATACGTTC GAAGAAATCA 
CGCCGTTCAT CCATGCCTGC CCGCAGTGAC AGTGGCAGCA GCCCCAGCAG CTGCAGCAGC 
TCCTCCTTTC ACTTCAGCAG CAGCAGCGAT GAGGATAGCA GTGACAGTAG TACCACCAAC 
TCCGAGCCAC CCAAGAAAAA GTCACGCAAG CGAGTGGCGA ATAATAAGCG CTCTAAAAAG 
GAGTCAATCG GGAAAAGGAC ATCTCGTAAA CGGAAGGCCA AAGACCAGAA TATGGAAATG 
CTTGAGCAGC AGCAGATAAG CCAAAAGAAG CCCCAGCGCC AACCGGAAAG CAGCAGTGAC 
AGTCCAAGTT CGTCCGATGA CGAGTCAGGT GGCGATAGCA GCGAGTCATC CGGTCAACCC 
AACACGAACA ACAACAAATC CAGCAGCGAT TCCGATGATG ATAGCGCTGT AAACATGCAG 
CTCAGTGCCT TGCGCGCCAC ACTAAAAGCG GAGGCAACTC TGGAGGATAG GAAACCTGTA 
AAACTGGAGC TTCAGTTGCC GGATGACGAC CAGGCGGGGC CCCTCCACTC ACGGATGACT 
CCATCGCCGA GAGAGGATAA CGAACCGGGT TGCAGTGCAC CGAAACGGCG ACGCAGTTGC 
AGCCACAGCA ATCAGTCCAG CCAGAGTGCC AGTTTGGCCA GCAGTTCGAC AGCCACCTCG 
AGCTCGGCTC CATTAAGTTC GTTTGCCTGG GGAGCAGCGG GCTTTTCCGG CGATCCGCTG 
ATGCGCGGAC ATCCGGCGAT GGAAGAGCAC GACATAGCCA ACTCCCTAAT CGAACTGTCC 
ACTCTAACGC AGCCTGTGAA CATCGGACTC TTCAACGAGC ATTATAATTC GGCAGAGAAT 
TCCATGGGCA TGCTGAGCAA CACGTTGTGC GACAGCCCAC AAACTTTGGC GGCGGATGAC 
GCCAATCTAG AGAACTATTT CGATACAGAT GCGGATCCGG AGGCCAGCCG TGAACGGGAT 
GCCTATTCGC TGGAGGCCGC AATCGATGTA GTTGGCGAAT CAGAGTTGCA AATCGCCGAG 
GATACAGCCA CTGCCACCGA AGAACAAGAT GAGGAGGACG AAGAGGATGA AGATCAAGAG 
GAAGACGACC AGGAAGAGGA AAAAGCTGCG GAGGAAGAAG AGGAAGAGGA GGAGGACGAC 
GATGATAGCG ACAACCACGA TGAAAACGAT GAGAATCAGG GACTACTGCC GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611388.1
CDS	401..3517
Translation	MAEENPGALAANVPYLGVDELGASVIVEPGLEGSNAGGRTLPAAAIKFADLTESGSESGDNEAEEPVSAGPDNANAIGEPGTSASAAEENGTVERNSPPPNCAICLSRCRRKCFTDSCMHQFCFKCLCEWSKIKPECPLCKQPFRTIIHNVRTLDDYDRYPVQTTSPVPTENPSLRYHIVRRPRYTPLVQNQAVIVNDIEAAIAAGAAGEDVLSAAEVAAGRRSYSRFEPYRSELMNYYQHDQDASTSGSLSQLWRRYVYDRKLYALPVSDSVTGHFREWSARFYRNNPAQIHRLMPWIHRDIMCLLRNAAHSVNTVMTLMSDLLPMTSLLGPTFRRRLSPYLGERTSHFIHELFNFARSPYDINGYDHVVQYSARVAEEVEVDLLDMVETQSSNGDDLNLEVGDSDADAINAGFSPDWSPPRVRPSTSVIVTNPGATHSFSVTMASDGSELPGISIRRTTNVGSQTVAINLSMRRPAAVASEEPEVIEIDDGDAAANAEVAAINDGSNTSRRHAGATLPVTAHIELESSSSSGDEDECVFVLELKPPHMRTPEQVSLDSNSDSDVVFVNEQHEAAPDAIAENRSTQSPLDLASRDQGLFMGPSTSGAAANRGKNWKLVMAQTRRLDQLRTLRSIRSKKSRRSSMPARSDSGSSPSSCSSSSFHFSSSSDEDSSDSSTTNSEPPKKKSRKRVANNKRSKKESIGKRTSRKRKAKDQNMEMLEQQQISQKKPQRQPESSSDSPSSSDDESGGDSSESSGQPNTNNNKSSSDSDDDSAVNMQLSALRATLKAEATLEDRKPVKLELQLPDDDQAGPLHSRMTPSPREDNEPGCSAPKRRRSCSHSNQSSQSASLASSSTATSSSAPLSSFAWGAAGFSGDPLMRGHPAMEEHDIANSLIELSTLTQPVNIGLFNEHYNSAENSMGMLSNTLCDSPQTLAADDANLENYFDTDADPEASRERDAYSLEAAIDVVGESELQIAEDTATATEEQDEEDEEDEDQEEDDQEEEKAAEEEEEEEEDDDDSDNHDENDENQGLLPY

Target ORF information:

RefSeq Version	NM_137544.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase I-interacting protein, transcript variant A (Topors), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137544.3

ORF Insert Sequence:

ATGGCGGAGG AGAATCCCGG AGCTCTTGCC GCCAACGTGC CCTATTTGGG AGTGGATGAG 
TTGGGCGCAT CGGTGATTGT GGAGCCTGGT TTGGAAGGAT CCAATGCCGG AGGCCGGACG 
CTACCGGCTG CGGCCATTAA GTTTGCTGAT CTCACCGAGA GCGGCAGTGA GTCGGGAGAC 
AACGAGGCCG AGGAACCTGT ATCCGCTGGC CCGGATAATG CCAACGCCAT CGGTGAGCCG 
GGCACCAGTG CATCCGCCGC GGAGGAGAAC GGAACAGTAG AGCGCAACTC GCCGCCGCCC 
AATTGCGCCA TCTGCTTGTC CCGCTGCAGG AGGAAGTGCT TCACGGACTC GTGCATGCAC 
CAGTTCTGCT TCAAGTGCCT GTGCGAGTGG AGCAAGATAA AGCCCGAGTG TCCACTGTGT 
AAGCAGCCCT TCAGAACCAT CATACACAAT GTCCGCACCC TGGACGACTA CGATCGCTAC 
CCGGTGCAGA CTACGTCGCC GGTTCCAACA GAAAATCCCT CCCTACGTTA CCACATTGTG 
AGGCGTCCCA GGTACACGCC GCTGGTGCAG AACCAGGCCG TCATTGTCAA CGACATCGAG 
GCGGCCATAG CGGCAGGAGC TGCTGGTGAA GATGTGCTTT CGGCGGCAGA AGTAGCAGCT 
GGCAGGCGAT CGTACAGCCG CTTTGAGCCG TATCGCTCGG AGCTGATGAA TTACTACCAG 
CACGATCAGG ATGCATCCAC GTCGGGTTCA TTGAGCCAGC TCTGGCGACG TTATGTGTAC 
GATAGAAAGC TGTATGCTCT GCCCGTCAGC GACAGCGTGA CTGGACACTT TCGAGAGTGG 
AGCGCCCGTT TTTACAGAAA CAATCCTGCT CAGATTCATC GCCTGATGCC TTGGATCCAT 
CGCGACATCA TGTGCCTACT GAGGAATGCC GCACATAGCG TAAATACTGT GATGACTCTG 
ATGAGCGACC TCCTGCCCAT GACTAGCTTA CTAGGTCCTA CATTCCGTCG ACGGCTGTCG 
CCGTATCTCG GCGAGCGCAC CAGTCACTTT ATCCACGAAC TGTTTAACTT TGCCCGCTCC 
CCGTATGACA TAAACGGTTA CGATCATGTG GTGCAGTATT CGGCACGAGT GGCTGAGGAG 
GTGGAGGTGG ATCTGTTGGA CATGGTGGAG ACACAATCCT CGAACGGTGA TGATCTGAAT 
TTGGAGGTCG GAGATAGCGA TGCGGATGCT ATAAACGCTG GCTTCTCTCC GGATTGGAGC 
CCCCCTAGGG TACGCCCGTC GACCAGTGTG ATTGTGACCA ATCCTGGTGC CACACATTCC 
TTCAGCGTGA CAATGGCCAG TGATGGCAGT GAGCTGCCGG GGATTTCCAT ACGCCGTACA 
ACCAATGTGG GCTCACAAAC GGTTGCCATT AACCTGAGCA TGAGGCGCCC AGCGGCGGTT 
GCCAGCGAGG AACCAGAGGT CATCGAGATC GACGACGGCG ATGCGGCCGC AAATGCAGAG 
GTGGCGGCCA TCAATGATGG CAGCAACACC AGTAGGAGAC ACGCAGGTGC TACTCTTCCA 
GTGACGGCGC ACATCGAACT GGAGAGCAGC AGCAGTTCCG GCGATGAGGA TGAGTGCGTC 
TTTGTGCTGG AGCTGAAGCC ACCACACATG CGCACTCCCG AACAGGTGAG CCTTGACTCA 
AATAGTGACT CCGATGTGGT GTTCGTAAAT GAGCAGCACG AAGCTGCGCC GGACGCAATT 
GCCGAAAACC GGTCGACACA ATCCCCTTTG GATCTGGCCT CCAGGGATCA GGGCCTGTTT 
ATGGGGCCCA GCACAAGTGG CGCAGCGGCC AACAGAGGCA AGAACTGGAA GCTGGTCATG 
GCGCAGACAC GGCGCCTCGA TCAGTTGCGC ACGCTGCGCT CTATACGTTC GAAGAAATCA 
CGCCGTTCAT CCATGCCTGC CCGCAGTGAC AGTGGCAGCA GCCCCAGCAG CTGCAGCAGC 
TCCTCCTTTC ACTTCAGCAG CAGCAGCGAT GAGGATAGCA GTGACAGTAG TACCACCAAC 
TCCGAGCCAC CCAAGAAAAA GTCACGCAAG CGAGTGGCGA ATAATAAGCG CTCTAAAAAG 
GAGTCAATCG GGAAAAGGAC ATCTCGTAAA CGGAAGGCCA AAGACCAGAA TATGGAAATG 
CTTGAGCAGC AGCAGATAAG CCAAAAGAAG CCCCAGCGCC AACCGGAAAG CAGCAGTGAC 
AGTCCAAGTT CGTCCGATGA CGAGTCAGGT GGCGATAGCA GCGAGTCATC CGGTCAACCC 
AACACGAACA ACAACAAATC CAGCAGCGAT TCCGATGATG ATAGCGCTGT AAACATGCAG 
CTCAGTGCCT TGCGCGCCAC ACTAAAAGCG GAGGCAACTC TGGAGGATAG GAAACCTGTA 
AAACTGGAGC TTCAGTTGCC GGATGACGAC CAGGCGGGGC CCCTCCACTC ACGGATGACT 
CCATCGCCGA GAGAGGATAA CGAACCGGGT TGCAGTGCAC CGAAACGGCG ACGCAGTTGC 
AGCCACAGCA ATCAGTCCAG CCAGAGTGCC AGTTTGGCCA GCAGTTCGAC AGCCACCTCG 
AGCTCGGCTC CATTAAGTTC GTTTGCCTGG GGAGCAGCGG GCTTTTCCGG CGATCCGCTG 
ATGCGCGGAC ATCCGGCGAT GGAAGAGCAC GACATAGCCA ACTCCCTAAT CGAACTGTCC 
ACTCTAACGC AGCCTGTGAA CATCGGACTC TTCAACGAGC ATTATAATTC GGCAGAGAAT 
TCCATGGGCA TGCTGAGCAA CACGTTGTGC GACAGCCCAC AAACTTTGGC GGCGGATGAC 
GCCAATCTAG AGAACTATTT CGATACAGAT GCGGATCCGG AGGCCAGCCG TGAACGGGAT 
GCCTATTCGC TGGAGGCCGC AATCGATGTA GTTGGCGAAT CAGAGTTGCA AATCGCCGAG 
GATACAGCCA CTGCCACCGA AGAACAAGAT GAGGAGGACG AAGAGGATGA AGATCAAGAG 
GAAGACGACC AGGAAGAGGA AAAAGCTGCG GAGGAAGAAG AGGAAGAGGA GGAGGACGAC 
GATGATAGCG ACAACCACGA TGAAAACGAT GAGAATCAGG GACTACTGCC GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topors ( NM_001274154.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261083.1 Drosophila melanogaster topoisomerase I-interacting protein, transcript variant B (Topors), mRNA.

Price & Availability↑

CloneID	OFa10561
Clone ID Related Accession (Same CDS sequence)	NM_137544.3 , NM_001274154.1
Accession Version	NM_001274154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase I-interacting protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGG AGAATCCCGG AGCTCTTGCC GCCAACGTGC CCTATTTGGG AGTGGATGAG 
TTGGGCGCAT CGGTGATTGT GGAGCCTGGT TTGGAAGGAT CCAATGCCGG AGGCCGGACG 
CTACCGGCTG CGGCCATTAA GTTTGCTGAT CTCACCGAGA GCGGCAGTGA GTCGGGAGAC 
AACGAGGCCG AGGAACCTGT ATCCGCTGGC CCGGATAATG CCAACGCCAT CGGTGAGCCG 
GGCACCAGTG CATCCGCCGC GGAGGAGAAC GGAACAGTAG AGCGCAACTC GCCGCCGCCC 
AATTGCGCCA TCTGCTTGTC CCGCTGCAGG AGGAAGTGCT TCACGGACTC GTGCATGCAC 
CAGTTCTGCT TCAAGTGCCT GTGCGAGTGG AGCAAGATAA AGCCCGAGTG TCCACTGTGT 
AAGCAGCCCT TCAGAACCAT CATACACAAT GTCCGCACCC TGGACGACTA CGATCGCTAC 
CCGGTGCAGA CTACGTCGCC GGTTCCAACA GAAAATCCCT CCCTACGTTA CCACATTGTG 
AGGCGTCCCA GGTACACGCC GCTGGTGCAG AACCAGGCCG TCATTGTCAA CGACATCGAG 
GCGGCCATAG CGGCAGGAGC TGCTGGTGAA GATGTGCTTT CGGCGGCAGA AGTAGCAGCT 
GGCAGGCGAT CGTACAGCCG CTTTGAGCCG TATCGCTCGG AGCTGATGAA TTACTACCAG 
CACGATCAGG ATGCATCCAC GTCGGGTTCA TTGAGCCAGC TCTGGCGACG TTATGTGTAC 
GATAGAAAGC TGTATGCTCT GCCCGTCAGC GACAGCGTGA CTGGACACTT TCGAGAGTGG 
AGCGCCCGTT TTTACAGAAA CAATCCTGCT CAGATTCATC GCCTGATGCC TTGGATCCAT 
CGCGACATCA TGTGCCTACT GAGGAATGCC GCACATAGCG TAAATACTGT GATGACTCTG 
ATGAGCGACC TCCTGCCCAT GACTAGCTTA CTAGGTCCTA CATTCCGTCG ACGGCTGTCG 
CCGTATCTCG GCGAGCGCAC CAGTCACTTT ATCCACGAAC TGTTTAACTT TGCCCGCTCC 
CCGTATGACA TAAACGGTTA CGATCATGTG GTGCAGTATT CGGCACGAGT GGCTGAGGAG 
GTGGAGGTGG ATCTGTTGGA CATGGTGGAG ACACAATCCT CGAACGGTGA TGATCTGAAT 
TTGGAGGTCG GAGATAGCGA TGCGGATGCT ATAAACGCTG GCTTCTCTCC GGATTGGAGC 
CCCCCTAGGG TACGCCCGTC GACCAGTGTG ATTGTGACCA ATCCTGGTGC CACACATTCC 
TTCAGCGTGA CAATGGCCAG TGATGGCAGT GAGCTGCCGG GGATTTCCAT ACGCCGTACA 
ACCAATGTGG GCTCACAAAC GGTTGCCATT AACCTGAGCA TGAGGCGCCC AGCGGCGGTT 
GCCAGCGAGG AACCAGAGGT CATCGAGATC GACGACGGCG ATGCGGCCGC AAATGCAGAG 
GTGGCGGCCA TCAATGATGG CAGCAACACC AGTAGGAGAC ACGCAGGTGC TACTCTTCCA 
GTGACGGCGC ACATCGAACT GGAGAGCAGC AGCAGTTCCG GCGATGAGGA TGAGTGCGTC 
TTTGTGCTGG AGCTGAAGCC ACCACACATG CGCACTCCCG AACAGGTGAG CCTTGACTCA 
AATAGTGACT CCGATGTGGT GTTCGTAAAT GAGCAGCACG AAGCTGCGCC GGACGCAATT 
GCCGAAAACC GGTCGACACA ATCCCCTTTG GATCTGGCCT CCAGGGATCA GGGCCTGTTT 
ATGGGGCCCA GCACAAGTGG CGCAGCGGCC AACAGAGGCA AGAACTGGAA GCTGGTCATG 
GCGCAGACAC GGCGCCTCGA TCAGTTGCGC ACGCTGCGCT CTATACGTTC GAAGAAATCA 
CGCCGTTCAT CCATGCCTGC CCGCAGTGAC AGTGGCAGCA GCCCCAGCAG CTGCAGCAGC 
TCCTCCTTTC ACTTCAGCAG CAGCAGCGAT GAGGATAGCA GTGACAGTAG TACCACCAAC 
TCCGAGCCAC CCAAGAAAAA GTCACGCAAG CGAGTGGCGA ATAATAAGCG CTCTAAAAAG 
GAGTCAATCG GGAAAAGGAC ATCTCGTAAA CGGAAGGCCA AAGACCAGAA TATGGAAATG 
CTTGAGCAGC AGCAGATAAG CCAAAAGAAG CCCCAGCGCC AACCGGAAAG CAGCAGTGAC 
AGTCCAAGTT CGTCCGATGA CGAGTCAGGT GGCGATAGCA GCGAGTCATC CGGTCAACCC 
AACACGAACA ACAACAAATC CAGCAGCGAT TCCGATGATG ATAGCGCTGT AAACATGCAG 
CTCAGTGCCT TGCGCGCCAC ACTAAAAGCG GAGGCAACTC TGGAGGATAG GAAACCTGTA 
AAACTGGAGC TTCAGTTGCC GGATGACGAC CAGGCGGGGC CCCTCCACTC ACGGATGACT 
CCATCGCCGA GAGAGGATAA CGAACCGGGT TGCAGTGCAC CGAAACGGCG ACGCAGTTGC 
AGCCACAGCA ATCAGTCCAG CCAGAGTGCC AGTTTGGCCA GCAGTTCGAC AGCCACCTCG 
AGCTCGGCTC CATTAAGTTC GTTTGCCTGG GGAGCAGCGG GCTTTTCCGG CGATCCGCTG 
ATGCGCGGAC ATCCGGCGAT GGAAGAGCAC GACATAGCCA ACTCCCTAAT CGAACTGTCC 
ACTCTAACGC AGCCTGTGAA CATCGGACTC TTCAACGAGC ATTATAATTC GGCAGAGAAT 
TCCATGGGCA TGCTGAGCAA CACGTTGTGC GACAGCCCAC AAACTTTGGC GGCGGATGAC 
GCCAATCTAG AGAACTATTT CGATACAGAT GCGGATCCGG AGGCCAGCCG TGAACGGGAT 
GCCTATTCGC TGGAGGCCGC AATCGATGTA GTTGGCGAAT CAGAGTTGCA AATCGCCGAG 
GATACAGCCA CTGCCACCGA AGAACAAGAT GAGGAGGACG AAGAGGATGA AGATCAAGAG 
GAAGACGACC AGGAAGAGGA AAAAGCTGCG GAGGAAGAAG AGGAAGAGGA GGAGGACGAC 
GATGATAGCG ACAACCACGA TGAAAACGAT GAGAATCAGG GACTACTGCC GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261083.1
CDS	657..3773
Translation	MAEENPGALAANVPYLGVDELGASVIVEPGLEGSNAGGRTLPAAAIKFADLTESGSESGDNEAEEPVSAGPDNANAIGEPGTSASAAEENGTVERNSPPPNCAICLSRCRRKCFTDSCMHQFCFKCLCEWSKIKPECPLCKQPFRTIIHNVRTLDDYDRYPVQTTSPVPTENPSLRYHIVRRPRYTPLVQNQAVIVNDIEAAIAAGAAGEDVLSAAEVAAGRRSYSRFEPYRSELMNYYQHDQDASTSGSLSQLWRRYVYDRKLYALPVSDSVTGHFREWSARFYRNNPAQIHRLMPWIHRDIMCLLRNAAHSVNTVMTLMSDLLPMTSLLGPTFRRRLSPYLGERTSHFIHELFNFARSPYDINGYDHVVQYSARVAEEVEVDLLDMVETQSSNGDDLNLEVGDSDADAINAGFSPDWSPPRVRPSTSVIVTNPGATHSFSVTMASDGSELPGISIRRTTNVGSQTVAINLSMRRPAAVASEEPEVIEIDDGDAAANAEVAAINDGSNTSRRHAGATLPVTAHIELESSSSSGDEDECVFVLELKPPHMRTPEQVSLDSNSDSDVVFVNEQHEAAPDAIAENRSTQSPLDLASRDQGLFMGPSTSGAAANRGKNWKLVMAQTRRLDQLRTLRSIRSKKSRRSSMPARSDSGSSPSSCSSSSFHFSSSSDEDSSDSSTTNSEPPKKKSRKRVANNKRSKKESIGKRTSRKRKAKDQNMEMLEQQQISQKKPQRQPESSSDSPSSSDDESGGDSSESSGQPNTNNNKSSSDSDDDSAVNMQLSALRATLKAEATLEDRKPVKLELQLPDDDQAGPLHSRMTPSPREDNEPGCSAPKRRRSCSHSNQSSQSASLASSSTATSSSAPLSSFAWGAAGFSGDPLMRGHPAMEEHDIANSLIELSTLTQPVNIGLFNEHYNSAENSMGMLSNTLCDSPQTLAADDANLENYFDTDADPEASRERDAYSLEAAIDVVGESELQIAEDTATATEEQDEEDEEDEDQEEDDQEEEKAAEEEEEEEEDDDDSDNHDENDENQGLLPY

Target ORF information:

RefSeq Version	NM_001274154.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase I-interacting protein, transcript variant B (Topors), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274154.1

ORF Insert Sequence:

ATGGCGGAGG AGAATCCCGG AGCTCTTGCC GCCAACGTGC CCTATTTGGG AGTGGATGAG 
TTGGGCGCAT CGGTGATTGT GGAGCCTGGT TTGGAAGGAT CCAATGCCGG AGGCCGGACG 
CTACCGGCTG CGGCCATTAA GTTTGCTGAT CTCACCGAGA GCGGCAGTGA GTCGGGAGAC 
AACGAGGCCG AGGAACCTGT ATCCGCTGGC CCGGATAATG CCAACGCCAT CGGTGAGCCG 
GGCACCAGTG CATCCGCCGC GGAGGAGAAC GGAACAGTAG AGCGCAACTC GCCGCCGCCC 
AATTGCGCCA TCTGCTTGTC CCGCTGCAGG AGGAAGTGCT TCACGGACTC GTGCATGCAC 
CAGTTCTGCT TCAAGTGCCT GTGCGAGTGG AGCAAGATAA AGCCCGAGTG TCCACTGTGT 
AAGCAGCCCT TCAGAACCAT CATACACAAT GTCCGCACCC TGGACGACTA CGATCGCTAC 
CCGGTGCAGA CTACGTCGCC GGTTCCAACA GAAAATCCCT CCCTACGTTA CCACATTGTG 
AGGCGTCCCA GGTACACGCC GCTGGTGCAG AACCAGGCCG TCATTGTCAA CGACATCGAG 
GCGGCCATAG CGGCAGGAGC TGCTGGTGAA GATGTGCTTT CGGCGGCAGA AGTAGCAGCT 
GGCAGGCGAT CGTACAGCCG CTTTGAGCCG TATCGCTCGG AGCTGATGAA TTACTACCAG 
CACGATCAGG ATGCATCCAC GTCGGGTTCA TTGAGCCAGC TCTGGCGACG TTATGTGTAC 
GATAGAAAGC TGTATGCTCT GCCCGTCAGC GACAGCGTGA CTGGACACTT TCGAGAGTGG 
AGCGCCCGTT TTTACAGAAA CAATCCTGCT CAGATTCATC GCCTGATGCC TTGGATCCAT 
CGCGACATCA TGTGCCTACT GAGGAATGCC GCACATAGCG TAAATACTGT GATGACTCTG 
ATGAGCGACC TCCTGCCCAT GACTAGCTTA CTAGGTCCTA CATTCCGTCG ACGGCTGTCG 
CCGTATCTCG GCGAGCGCAC CAGTCACTTT ATCCACGAAC TGTTTAACTT TGCCCGCTCC 
CCGTATGACA TAAACGGTTA CGATCATGTG GTGCAGTATT CGGCACGAGT GGCTGAGGAG 
GTGGAGGTGG ATCTGTTGGA CATGGTGGAG ACACAATCCT CGAACGGTGA TGATCTGAAT 
TTGGAGGTCG GAGATAGCGA TGCGGATGCT ATAAACGCTG GCTTCTCTCC GGATTGGAGC 
CCCCCTAGGG TACGCCCGTC GACCAGTGTG ATTGTGACCA ATCCTGGTGC CACACATTCC 
TTCAGCGTGA CAATGGCCAG TGATGGCAGT GAGCTGCCGG GGATTTCCAT ACGCCGTACA 
ACCAATGTGG GCTCACAAAC GGTTGCCATT AACCTGAGCA TGAGGCGCCC AGCGGCGGTT 
GCCAGCGAGG AACCAGAGGT CATCGAGATC GACGACGGCG ATGCGGCCGC AAATGCAGAG 
GTGGCGGCCA TCAATGATGG CAGCAACACC AGTAGGAGAC ACGCAGGTGC TACTCTTCCA 
GTGACGGCGC ACATCGAACT GGAGAGCAGC AGCAGTTCCG GCGATGAGGA TGAGTGCGTC 
TTTGTGCTGG AGCTGAAGCC ACCACACATG CGCACTCCCG AACAGGTGAG CCTTGACTCA 
AATAGTGACT CCGATGTGGT GTTCGTAAAT GAGCAGCACG AAGCTGCGCC GGACGCAATT 
GCCGAAAACC GGTCGACACA ATCCCCTTTG GATCTGGCCT CCAGGGATCA GGGCCTGTTT 
ATGGGGCCCA GCACAAGTGG CGCAGCGGCC AACAGAGGCA AGAACTGGAA GCTGGTCATG 
GCGCAGACAC GGCGCCTCGA TCAGTTGCGC ACGCTGCGCT CTATACGTTC GAAGAAATCA 
CGCCGTTCAT CCATGCCTGC CCGCAGTGAC AGTGGCAGCA GCCCCAGCAG CTGCAGCAGC 
TCCTCCTTTC ACTTCAGCAG CAGCAGCGAT GAGGATAGCA GTGACAGTAG TACCACCAAC 
TCCGAGCCAC CCAAGAAAAA GTCACGCAAG CGAGTGGCGA ATAATAAGCG CTCTAAAAAG 
GAGTCAATCG GGAAAAGGAC ATCTCGTAAA CGGAAGGCCA AAGACCAGAA TATGGAAATG 
CTTGAGCAGC AGCAGATAAG CCAAAAGAAG CCCCAGCGCC AACCGGAAAG CAGCAGTGAC 
AGTCCAAGTT CGTCCGATGA CGAGTCAGGT GGCGATAGCA GCGAGTCATC CGGTCAACCC 
AACACGAACA ACAACAAATC CAGCAGCGAT TCCGATGATG ATAGCGCTGT AAACATGCAG 
CTCAGTGCCT TGCGCGCCAC ACTAAAAGCG GAGGCAACTC TGGAGGATAG GAAACCTGTA 
AAACTGGAGC TTCAGTTGCC GGATGACGAC CAGGCGGGGC CCCTCCACTC ACGGATGACT 
CCATCGCCGA GAGAGGATAA CGAACCGGGT TGCAGTGCAC CGAAACGGCG ACGCAGTTGC 
AGCCACAGCA ATCAGTCCAG CCAGAGTGCC AGTTTGGCCA GCAGTTCGAC AGCCACCTCG 
AGCTCGGCTC CATTAAGTTC GTTTGCCTGG GGAGCAGCGG GCTTTTCCGG CGATCCGCTG 
ATGCGCGGAC ATCCGGCGAT GGAAGAGCAC GACATAGCCA ACTCCCTAAT CGAACTGTCC 
ACTCTAACGC AGCCTGTGAA CATCGGACTC TTCAACGAGC ATTATAATTC GGCAGAGAAT 
TCCATGGGCA TGCTGAGCAA CACGTTGTGC GACAGCCCAC AAACTTTGGC GGCGGATGAC 
GCCAATCTAG AGAACTATTT CGATACAGAT GCGGATCCGG AGGCCAGCCG TGAACGGGAT 
GCCTATTCGC TGGAGGCCGC AATCGATGTA GTTGGCGAAT CAGAGTTGCA AATCGCCGAG 
GATACAGCCA CTGCCACCGA AGAACAAGAT GAGGAGGACG AAGAGGATGA AGATCAAGAG 
GAAGACGACC AGGAAGAGGA AAAAGCTGCG GAGGAAGAAG AGGAAGAGGA GGAGGACGAC 
GATGATAGCG ACAACCACGA TGAAAACGAT GAGAATCAGG GACTACTGCC GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Topors cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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