Hsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsf
Entrez Gene ID	37068
Full Name	Heat shock factor
Synonyms	CG5748,D-HSF,Dm-Hsf,DmHSF,DmHSFa,Dmel\CG5748,HSF,HSF1,HSFa,Hsf1,dHSF,dHSFa,hsf,l(2)03091
General protein information	Preferred Names Heat shock factor Names CG5748 gene product from transcript CG5748-RA CG5748-PA CG5748-PB CG5748-PC CG5748-PD Heat-Shock factor Hsf-PA Hsf-PB Hsf-PC Hsf-PD heat shock factor heat shock transcription factor heat shock transcription factor 1 heat-shock transcription factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55B7-55B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043092.3	NP_001036557.1	heat shock factor, isoform B
NM_057227.5	NP_476575.1	heat shock factor, isoform A
NM_001043091.3	NP_001036556.1	heat shock factor, isoform C
NM_001043090.3	NP_001036555.1	heat shock factor, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsf	NP_001036556.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011082	XP_309567.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSF is dispensable for establishing or maintaining Pol II pausing but is critical for the release of paused Pol II into the gene body at a subset of highly activated genes
Title: Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation.

Knockdown of the Drosophila Nrf1 and Nrf2 ortholog cap 'n' collar isoform-C, but not Hsf1, blocks the protective effect of ASC-JM17 on mutant AR-induced eye degeneration in flies. Our observations indicate that activation of the Nrf1/Nrf2 pathway is a viable option for pharmacological intervention in SBMA and potentially other polyglutamine diseases.
Title: A small-molecule Nrf1 and Nrf2 activator mitigates polyglutamine toxicity in spinal and bulbar muscular atrophy.

HSF role in the heat shock response to viral infection
Title: The heat shock response restricts virus infection in Drosophila.

The survival curves of heterozygotes on hsf-1 and hsf-4 alleles are similar in spite of HSF-4 protein is slightly active on normal temperature.
Title: [VARIOUS ALLELES OF HSF HEAT-SHOCK TRANSCRIPTION FACTOR OF DROSOPHILA MELANOGASTER INCREASE VIABILITY OF ITS CARRIERS IN UNFAVORABLE ENVIRONMENTS].

Physiological function of mutated HSF4 is not fully abrogated after heat exposure and is able to recover even after severe heat stress, causing the induction of heat-shock gene expression.
Title: [Kinetics of heat shock response upon disfunction of general transcription factor (HSF)].

The following Hsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10433	NM_057227.5 Latest version!	Drosophila melanogaster heat shock factor, transcript variant A (Hsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10434	NM_001043091.3 Latest version!	Drosophila melanogaster heat shock factor, transcript variant C (Hsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10435	NM_001043092.3 Latest version!	Drosophila melanogaster heat shock factor, transcript variant B (Hsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10436	NM_001043090.3 Latest version!	Drosophila melanogaster heat shock factor, transcript variant D (Hsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsf ( NM_057227.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476575.1 Drosophila melanogaster heat shock factor, transcript variant A (Hsf), mRNA.

Price & Availability↑

CloneID	OFa10433
Clone ID Related Accession (Same CDS sequence)	NM_057227.5
Accession Version	NM_057227.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057227.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTTGACGAAC TCCACGGGCA CTTGGAAAGC ATGCAAGATG AGTTGGAAAC ACTGAAGGAT 
CTGCTGCGTG GCGATGGGGT GGCCATTGAT CAGAACATGC TCATGGGTCT GTTTAACGAC 
TCTGATCTAA TGGACAACTA TGGCCTATCG TTTCCCAATG ACAGCATAAG CAGTGAAAAG 
AAAGCACCCA GTGGCTCTGA ACTGATTTCC TATCAGCCCA TGTATGATCT GTCCGACATT 
TTGGACACGG ACGATGGCAA CAATGACCAG GAGGCCAGCA GGCGCCAGAT GCAGACGCAA 
AGTTCGGTTT TAAATACGCC ACGTCACGAG TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476575.1
CDS	244..2319
Translation	MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL

Target ORF information:

RefSeq Version	NM_057227.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock factor, transcript variant A (Hsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057227.5

ORF Insert Sequence:

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTTGACGAAC TCCACGGGCA CTTGGAAAGC ATGCAAGATG AGTTGGAAAC ACTGAAGGAT 
CTGCTGCGTG GCGATGGGGT GGCCATTGAT CAGAACATGC TCATGGGTCT GTTTAACGAC 
TCTGATCTAA TGGACAACTA TGGCCTATCG TTTCCCAATG ACAGCATAAG CAGTGAAAAG 
AAAGCACCCA GTGGCTCTGA ACTGATTTCC TATCAGCCCA TGTATGATCT GTCCGACATT 
TTGGACACGG ACGATGGCAA CAATGACCAG GAGGCCAGCA GGCGCCAGAT GCAGACGCAA 
AGTTCGGTTT TAAATACGCC ACGTCACGAG TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf ( NM_001043091.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036556.1 Drosophila melanogaster heat shock factor, transcript variant C (Hsf), mRNA.

Price & Availability↑

CloneID	OFa10434
Clone ID Related Accession (Same CDS sequence)	NM_001043091.3
Accession Version	NM_001043091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043091.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTTGACGAAC TCCACGGGCA CTTGGAAAGC ATGCAAGATG AGTTGGAAAC ACTGAAGGAT 
CTGCTGCGTG GCGATGGGGT GGCCATTGAT CAGAACATGC TCATGGGTGC GCTTAGCAGG 
ACGCCCAATT TTCAGTTGCC GGAGGACGAG CTGCTTTTAA AGCTGTTTAA CGACTCTGAT 
CTAATGGACA ACTATGGCCT ATCGTTTCCC AATGACAGCA TAAGCAGTGA AAAGAAAGCA 
CCCAGTGGCT CTGAACTGAT TTCCTATCAG CCCATGTATG ATCTGTCCGA CATTTTGGAC 
ACGGACGATG GCAACAATGA CCAGGAGGCC AGCAGGCGCC AGATGCAGAC GCAAAGTTCG 
GTTTTAAATA CGCCACGTCA CGAGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036556.1
CDS	257..2386
Translation	MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGALSRTPNFQLPEDELLLKLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL

Target ORF information:

RefSeq Version	NM_001043091.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock factor, transcript variant C (Hsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043091.3

ORF Insert Sequence:

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTTGACGAAC TCCACGGGCA CTTGGAAAGC ATGCAAGATG AGTTGGAAAC ACTGAAGGAT 
CTGCTGCGTG GCGATGGGGT GGCCATTGAT CAGAACATGC TCATGGGTGC GCTTAGCAGG 
ACGCCCAATT TTCAGTTGCC GGAGGACGAG CTGCTTTTAA AGCTGTTTAA CGACTCTGAT 
CTAATGGACA ACTATGGCCT ATCGTTTCCC AATGACAGCA TAAGCAGTGA AAAGAAAGCA 
CCCAGTGGCT CTGAACTGAT TTCCTATCAG CCCATGTATG ATCTGTCCGA CATTTTGGAC 
ACGGACGATG GCAACAATGA CCAGGAGGCC AGCAGGCGCC AGATGCAGAC GCAAAGTTCG 
GTTTTAAATA CGCCACGTCA CGAGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf ( NM_001043092.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036557.1 Drosophila melanogaster heat shock factor, transcript variant B (Hsf), mRNA.

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CloneID	OFa10435
Clone ID Related Accession (Same CDS sequence)	NM_001043092.3
Accession Version	NM_001043092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTGTTAGTAG ACCAGAAGAA TACGAATTCA AGTCGGATAA GCCATAATCA AGCGGCTAAT 
TCTTACTGTT GCAGTGACGA ACTCCACGGG CACTTGGAAA GCATGCAAGA TGAGTTGGAA 
ACACTGAAGG ATCTGCTGCG TGGCGATGGG GTGGCCATTG ATCAGAACAT GCTCATGGGT 
CTGTTTAACG ACTCTGATCT AATGGACAAC TATGGCCTAT CGTTTCCCAA TGACAGCATA 
AGCAGTGAAA AGAAAGCACC CAGTGGCTCT GAACTGATTT CCTATCAGCC CATGTATGAT 
CTGTCCGACA TTTTGGACAC GGACGATGGC AACAATGACC AGGAGGCCAG CAGGCGCCAG 
ATGCAGACGC AAAGTTCGGT TTTAAATACG CCACGTCACG AGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036557.1
CDS	244..2391
Translation	MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVLVDQKNTNSSRISHNQAANSYCCSDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL

Target ORF information:

RefSeq Version	NM_001043092.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock factor, transcript variant B (Hsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043092.3

ORF Insert Sequence:

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTGTTAGTAG ACCAGAAGAA TACGAATTCA AGTCGGATAA GCCATAATCA AGCGGCTAAT 
TCTTACTGTT GCAGTGACGA ACTCCACGGG CACTTGGAAA GCATGCAAGA TGAGTTGGAA 
ACACTGAAGG ATCTGCTGCG TGGCGATGGG GTGGCCATTG ATCAGAACAT GCTCATGGGT 
CTGTTTAACG ACTCTGATCT AATGGACAAC TATGGCCTAT CGTTTCCCAA TGACAGCATA 
AGCAGTGAAA AGAAAGCACC CAGTGGCTCT GAACTGATTT CCTATCAGCC CATGTATGAT 
CTGTCCGACA TTTTGGACAC GGACGATGGC AACAATGACC AGGAGGCCAG CAGGCGCCAG 
ATGCAGACGC AAAGTTCGGT TTTAAATACG CCACGTCACG AGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf ( NM_001043090.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036555.1 Drosophila melanogaster heat shock factor, transcript variant D (Hsf), mRNA.

Price & Availability↑

CloneID	OFa10436
Clone ID Related Accession (Same CDS sequence)	NM_001043090.3
Accession Version	NM_001043090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043090.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTGTTAGTAG ACCAGAAGAA TACGAATTCA AGTCGGATAA GCCATAATCA AGCGGCTAAT 
TCTTACTGTT GCAGTGACGA ACTCCACGGG CACTTGGAAA GCATGCAAGA TGAGTTGGAA 
ACACTGAAGG ATCTGCTGCG TGGCGATGGG GTGGCCATTG ATCAGAACAT GCTCATGGGT 
GCGCTTAGCA GGACGCCCAA TTTTCAGTTG CCGGAGGACG AGCTGCTTTT AAAGCTGTTT 
AACGACTCTG ATCTAATGGA CAACTATGGC CTATCGTTTC CCAATGACAG CATAAGCAGT 
GAAAAGAAAG CACCCAGTGG CTCTGAACTG ATTTCCTATC AGCCCATGTA TGATCTGTCC 
GACATTTTGG ACACGGACGA TGGCAACAAT GACCAGGAGG CCAGCAGGCG CCAGATGCAG 
ACGCAAAGTT CGGTTTTAAA TACGCCACGT CACGAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036555.1
CDS	244..2445
Translation	MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVLVDQKNTNSSRISHNQAANSYCCSDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGALSRTPNFQLPEDELLLKLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL

Target ORF information:

RefSeq Version	NM_001043090.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock factor, transcript variant D (Hsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043090.3

ORF Insert Sequence:

ATGTCCAGGT CGCGTTCATC CGCTAAAGCC GTGCAGTTCA AGCACGAATC GGAGGAAGAG 
GAAGAAGACG AGGAGGAGCA GCTGCCTTCC AGGAGAATGC ACTCTTACGG AGACGCCGCG 
GCCATCGGAA GCGGGGTGCC GGCCTTTTTG GCCAAATTGT GGCGCCTGGT GGACGATGCC 
GATACCAATC GCTTGATTTG CTGGACCAAG GATGGCCAAA GTTTCGTTAT ACAAAATCAA 
GCGCAATTCG CCAAGGAACT ATTGCCACTA AACTACAAGC ACAACAACAT GGCCAGCTTC 
ATAAGGCAAT TGAATATGTA TGGATTCCAC AAGATCACCT CTATTGACAA TGGCGGACTA 
CGTTTTGATC GCGACGAGAT TGAATTTTCG CACCCATTTT TTAAGCGCAA CTCGCCTTTT 
CTACTTGACC AAATCAAAAG GAAAATATCG AACAACAAAA ATGGTGACGA CAAAGGTGTC 
CTGAAGCCGG AGGCCATGTC GAAGATTCTC ACCGATGTGA AAGTCATGCG GGGTCGTCAG 
GACAATCTGG ATTCGCGCTT CTCCGCCATG AAACAGGAGA ACGAAGTGCT GTGGCGCGAG 
ATAGCCAGCC TGCGCCAAAA GCACGCTAAG CAGCAACAAA TAGTCAACAA ACTGATCCAG 
TTCCTCATTA CCATTGTGCA ACCGTCGCGC AACATGTCTG GCGTGAAACG CCACGTGCAG 
CTGATGATCA ACAATACGCC GGAAATTGAT CGTGCACGGA CCACCAGTGA GACCGAGAGC 
GAGAGTGGCG GCGGACCGGT TATCCACGAG CTTAGGGAGG AGCTTCTTGA TGAGGTGATG 
AATCCATCAC CGGCTGGCTA CACCGCAGCC TCACATTATG ACCAAGAGAG CGTCTCTCCG 
CCTGCCGTTG AGCGTCCGCG ATCTAACATG AGCATTAGCT CGCACAACGT CGATTATTCG 
AATCAGAGTG TGGAGGACTT GCTGCTCCAG GGAAATGGAA CCGCTGGCGG TAATATTCTA 
GTAGGCGGAG CCGCTTCTCC CATGGCCCAA AGTGTGAGTC AATCGCCGGC CCAACATGAT 
GTCTACACAG TCACCGAGGC GCCCGATTCT CATGTCCAGG AGGTGCCAAA CAGTCCGCCT 
TATTACGAGG AGCAGAATGT GCTTACCACG CCCATGGTGC GGGAGCAGGA GCAGCAGAAG 
CGTCAGCAGC TTAAGGAGAA CAACAAGCTA CGACGACAGG CAGGAGATGT TATACTTGAT 
GCTGGAGATA TTCTCGTAGA TAGTTCGTCG CCCAAGGCGC AACGGACAAG CATCCAGCAT 
AGTACGCAAC CTGATGTGAT GGTCCAGCCA ATGATTATAA AGTCTGAGCC GGAGAACAGT 
TCCGGACTGA TGGATCTAAT GACTCCCGCG AACGATCTGT ACAGTGTTAA CTTCATCAGT 
GAGGATATGC CGACGGATAT TTTTGAAGAC GCTCTGCTTC CCGACGGCGT GGAAGAGGCA 
GCCAAACTGG ACCAGCAACA AAAATTTGGG CAATCGACAG TGAGCAGCGG CAAGTTTGCC 
AGCAACTTCG ATGTGCCCAC CAACAGTACG CTGCTGGATG CCAATCAGGC CTCGACATCG 
AAGGCAGCGG CCAAGGCGCA AGCATCTGAG GAAGAGGGCA TGGCTGTGGC CAAATACAGT 
GGCGCTGAGA ACGGAAACAA CCGCGATACC AACAACAGTC AACTCCTCAG GATGGCCTCA 
GTGTTAGTAG ACCAGAAGAA TACGAATTCA AGTCGGATAA GCCATAATCA AGCGGCTAAT 
TCTTACTGTT GCAGTGACGA ACTCCACGGG CACTTGGAAA GCATGCAAGA TGAGTTGGAA 
ACACTGAAGG ATCTGCTGCG TGGCGATGGG GTGGCCATTG ATCAGAACAT GCTCATGGGT 
GCGCTTAGCA GGACGCCCAA TTTTCAGTTG CCGGAGGACG AGCTGCTTTT AAAGCTGTTT 
AACGACTCTG ATCTAATGGA CAACTATGGC CTATCGTTTC CCAATGACAG CATAAGCAGT 
GAAAAGAAAG CACCCAGTGG CTCTGAACTG ATTTCCTATC AGCCCATGTA TGATCTGTCC 
GACATTTTGG ACACGGACGA TGGCAACAAT GACCAGGAGG CCAGCAGGCG CCAGATGCAG 
ACGCAAAGTT CGGTTTTAAA TACGCCACGT CACGAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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