Dlish cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dlish
Entrez Gene ID	37014
Full Name	CG10933 gene product from transcript CG10933-RA
Synonyms	Dmel\CG10933
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E2-54E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_137407.3	NP_611251.2	dachs ligand with SH3s

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005616	XP_315625.4
Drosophila melanogaster (fruit fly)	CG10933	NP_611251.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dlish (CG10933) tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs
Title: The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.

The following Dlish gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dlish cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10374	NM_137407.3 Latest version!	Drosophila melanogaster dachs ligand with SH3s (Dlish), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dlish ( NM_137407.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611251.2 Drosophila melanogaster dachs ligand with SH3s (Dlish), mRNA.

Price & Availability↑

CloneID	OFa10374
Clone ID Related Accession (Same CDS sequence)	NM_137407.3
Accession Version	NM_137407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dachs ligand with SH3s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137407.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTTC TTTGCCCCGT GCGCATGAGG CGCGACAAGA AGAAAGCTAC AAATGCCAGC 
ATTGAGAGGG ACTTACCAGC TGTGGGTGTA TTGGGCATGG GCCGCATAAC TGGTTCCTCC 
AGCATTGAAA CACTTGTAAG AGTGGGCATT GAAAAGGAAC ACGGTCTAAG TCCTGATTCC 
AAGATGGTTG TGCTGCACGA CTTTACTCCT TGCGTGGATG ATGAACTAGA GGTGAAGCGT 
GGACAGCTGG TTAATATCTT GTACAGAGAG AACGATTGGG TCTACGTGAT TGGTCAGGAC 
TCTAGGCAGG AGGGTTTCAT ACCCTTCTCT TATTGTGCTC CCTGCAACAC GCAACTGGCG 
GATTTGGCTG TTAAGAAGAA ACTGCCAAGG GAACAGTGTC CAGAGCAGCC GATCGAAGAG 
AATATACCAC TTTTGGGCAC GGATAACAAG CTGGATGTTC TTTGCGATGA AACCTTGAAT 
CCTGGCTCCG CCAACAGCAT AGAGAACACC CTGCTGGTGG AGCCAGAGTG TACTCCTTTC 
GTGAAGGAAC CCTCCGGGCG CTGTATCGTC TTGTACACCT TTATAGCCAG GGACGAGAAT 
GATTTGTCTG TGGAACGGGG TGAATTTGTT ACCGTGCTAA ATCGCGAGGA TCCCGATTGG 
TTTTGGATAA TGCGCAGTGA TGGCCAGGAA GGATTTGTGC CAGCCAGCTT TATTTATCCT 
GCAGACAGTG TACGAGTTTT GCAGCAACAG AAAGCCACTT TAAATGCCAT GGAGACCATT 
TTGCAGCAGG GTCAACAAGG TCAGCAGAGC CAGCAACAGC AGCAGCCTCA ATTGGGCTTG 
GGAACAGATG ATCTACGCTA CCATGGCACG GAGCTGGTGA TGCTGTATGA TTACAAAGCG 
CAAGCTCCAG ACGATCTGTA CCTTTCTGTG CGGAGAGGAG ATTGGATTTA CGCAGACTTA 
ACCAATCAGA CTGTTGATGG CTGGCTTTGG GCTTATGCTC CGAAAACCCG CAAATATGGA 
TTCATCCCGA AAGCCTATGC CCGACCACCC GCGATGACCA GCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611251.2
CDS	132..1199
Translation	MAFLCPVRMRRDKKKATNASIERDLPAVGVLGMGRITGSSSIETLVRVGIEKEHGLSPDSKMVVLHDFTPCVDDELEVKRGQLVNILYRENDWVYVIGQDSRQEGFIPFSYCAPCNTQLADLAVKKKLPREQCPEQPIEENIPLLGTDNKLDVLCDETLNPGSANSIENTLLVEPECTPFVKEPSGRCIVLYTFIARDENDLSVERGEFVTVLNREDPDWFWIMRSDGQEGFVPASFIYPADSVRVLQQQKATLNAMETILQQGQQGQQSQQQQQPQLGLGTDDLRYHGTELVMLYDYKAQAPDDLYLSVRRGDWIYADLTNQTVDGWLWAYAPKTRKYGFIPKAYARPPAMTSL

Target ORF information:

RefSeq Version	NM_137407.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dachs ligand with SH3s (Dlish), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137407.3

ORF Insert Sequence:

ATGGCATTTC TTTGCCCCGT GCGCATGAGG CGCGACAAGA AGAAAGCTAC AAATGCCAGC 
ATTGAGAGGG ACTTACCAGC TGTGGGTGTA TTGGGCATGG GCCGCATAAC TGGTTCCTCC 
AGCATTGAAA CACTTGTAAG AGTGGGCATT GAAAAGGAAC ACGGTCTAAG TCCTGATTCC 
AAGATGGTTG TGCTGCACGA CTTTACTCCT TGCGTGGATG ATGAACTAGA GGTGAAGCGT 
GGACAGCTGG TTAATATCTT GTACAGAGAG AACGATTGGG TCTACGTGAT TGGTCAGGAC 
TCTAGGCAGG AGGGTTTCAT ACCCTTCTCT TATTGTGCTC CCTGCAACAC GCAACTGGCG 
GATTTGGCTG TTAAGAAGAA ACTGCCAAGG GAACAGTGTC CAGAGCAGCC GATCGAAGAG 
AATATACCAC TTTTGGGCAC GGATAACAAG CTGGATGTTC TTTGCGATGA AACCTTGAAT 
CCTGGCTCCG CCAACAGCAT AGAGAACACC CTGCTGGTGG AGCCAGAGTG TACTCCTTTC 
GTGAAGGAAC CCTCCGGGCG CTGTATCGTC TTGTACACCT TTATAGCCAG GGACGAGAAT 
GATTTGTCTG TGGAACGGGG TGAATTTGTT ACCGTGCTAA ATCGCGAGGA TCCCGATTGG 
TTTTGGATAA TGCGCAGTGA TGGCCAGGAA GGATTTGTGC CAGCCAGCTT TATTTATCCT 
GCAGACAGTG TACGAGTTTT GCAGCAACAG AAAGCCACTT TAAATGCCAT GGAGACCATT 
TTGCAGCAGG GTCAACAAGG TCAGCAGAGC CAGCAACAGC AGCAGCCTCA ATTGGGCTTG 
GGAACAGATG ATCTACGCTA CCATGGCACG GAGCTGGTGA TGCTGTATGA TTACAAAGCG 
CAAGCTCCAG ACGATCTGTA CCTTTCTGTG CGGAGAGGAG ATTGGATTTA CGCAGACTTA 
ACCAATCAGA CTGTTGATGG CTGGCTTTGG GCTTATGCTC CGAAAACCCG CAAATATGGA 
TTCATCCCGA AAGCCTATGC CCGACCACCC GCGATGACCA GCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dlish cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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