RhoGAP54D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP54D
Entrez Gene ID	36996
Full Name	Rho GTPase activating protein at 54D
Synonyms	CG6477,Dmel\CG6477
General protein information	Preferred Names Rho GTPase activating protein at 54D Names CG6477 gene product from transcript CG6477-RA CG6477-PA CG6477-PC RhoGAP54D-PA RhoGAP54D-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54C11-54C12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274131.2	NP_001261060.1	Rho GTPase activating protein at 54D, isoform C
NM_137392.3	NP_611236.2	Rho GTPase activating protein at 54D, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RhoGAP54D	NP_611236.2
Xenopus tropicalis (tropical clawed frog)	arhgap19	XP_002939704.1
Homo sapiens (human)	ARHGAP19	NP_116289.4
Canis lupus familiaris (dog)	ARHGAP19	XP_534979.3
Rattus norvegicus (Norway rat)	Arhgap19	XP_003749164.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001687	XP_321406.5
Pan troglodytes (chimpanzee)	ARHGAP19	XP_001161248.1
	ARHGAP19	XP_001101897.1
Bos taurus (cattle)	ARHGAP19	XP_002698528.1
Mus musculus (house mouse)	Arhgap19	NP_081943.2
Gallus gallus (chicken)	ARHGAP19	NP_001012927.1
Danio rerio (zebrafish)	arhgap19	XP_003201186.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP54D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP54D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10360	NM_137392.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10360	NM_001274131.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP54D ( NM_001274131.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261060.1 Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10360
Clone ID Related Accession (Same CDS sequence)	NM_001274131.2 , NM_137392.3
Accession Version	NM_001274131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 54D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274131.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGCAA CGATGGATAC GGACCATGCG AACCTAAATG ACTTCAGGGA TCGCGATCTG 
GCCACGCGCA TGCGCCGCTG CAATTACGAA AAATACAAAT CCTTGGTGCG CATGCACCTA 
TCGTTTGAAT TGGAGCTCAA TACGGATGAA TTCGATCTCC CCTGTCACGA GATTGTCTAC 
GAGGACAAGG GCAAGCTGAA GAAGTGGAAC CGCCTGTCTA AAAAACATCG TTTGGGACAT 
GGAGCAGCTG CCACATCCTG TGCCGCCGGC TCCGCCAGTA AATCCGTGGG TAATAGTCCC 
ACAGAGACAC TGCAACAGCA GATTGATCCG GGATTCTTAA TGCATCTGCA AGAGCTCAAG 
GAGTTCCTAA TGCTGGAGAA GAATCTCACC CAGGAAGGCC TATTCCGCAA GGCTGGTGCG 
GTGTCCCGAC AAAACGAACT TCGGATGCAC ATCCAGCACG ACAAACCACT GAATTTGGAA 
CTTGCGGGCT TCTCCGCTCA CGATTGCGCG ACCGTTTTCA AAGGTTTTCT ATCCGAACTT 
CCAGAACCGC TGCTTACGGA TGCCCACTAT CCGGCGCATC TGCAGATAGC CCCACTCTGC 
CAGGCGCTCA ATGGTCAGAC GACTGCCACT GCTGAGCGTC AACAACATCT TCTCAACTCC 
GTGCAGCTGC TACTTCTCCT CCTGCCCGAA GAACATCGTG AACTTCTTCA GCACATCATT 
GAGATGCTGC ATGCTGTGGC CAAGCATGAG AAAAGCAACA AGATGAGCGC CGATAACTTG 
GCCACTCTCT TCACACCGCA CCTAATTTGC CCAAGGCAAC TGCCACCCGA GGTCCTGCAC 
TACCAGGCCA AGAAAATGTC CAGCATCGTG ACCTATATGA TCGTCCGTGG ACTGGACATC 
TTCGAGGTGC CTGGAAAGCT GTCTACCGAC ATCCGGGCGT ACTTTCTCGA GCGCAAAAGA 
AAGAAAACCA TGTCGCCGGA ACAAACGCTT GACGAGTCCA TCTCGGACGT CTCGACGGTC 
AACACGGTGT ACACTTTTGT AGACCGAGCC GCCACTGCAG CGGCCACCAA TACGAACAAT 
ACGGACACGG AGCTGGCGCA ACTTTATGCC CATATCCAGA GCATGCCGGA GTCCTCTAAG 
AAGAGGCGTT TGATTAAGCA GTTCAACAAG CAAAATGGGC AGGGCACACC GTTGCAACTG 
GTGGTAATGA ATCGTTTGAA GAACAACGAG GCCACTCGAA GTGCCAAGTC CCTGGGAGAT 
TCCATTAAGA AGCACATTTT CCACAAGAGC CTCATGTCGC GGACTCCAAA AAGAGTGCCA 
CCCAGTTTTC ATTTGGCTAG TGGATCCGAG ACGCCCAATA TGTCGCATGT GAAACCGCCC 
AAGATGCGGG TGCTTTTTCA GAGCCCCACG CCACCCACAC CCACCTCATC GACCATCACC 
AGCGTCAGCC TGTCCACTAA CACTCGGCAT CAGCTTCAGA AATCCATCTC ATCTGCTTCC 
CTCAAGATCG AATCCTCGTC TTCGGACAGC AGCTCTAGCT GTGCGACCAG CGGTAGTATC 
TCTGCTCCAG TCAGTCGACA GCAATCGAAA GAATCGCCTT CTGGCAATGG AGCTGCTGGT 
GCTTCCGGCC AGGAACTGGA TGAGATCGAT GCCGATTGCT GCGTGACGCC TTTAAAGATC 
ATGTCAGCGG TTGCAAAGCA GCTAATTGAC AAGAAGAGCG TTGCCTGGGA CGAGCACCGA 
CTGCTGGAGA TTGAGGAGTA CTCGAATCCC TCAACGCCCG TTCAGCAATC GACGCGCTAT 
AAGTCGGAGC CTAATCTGTC AAGTATCCTG CCGCAGGTGG ACGCAGAGGA AGAGGACGAC 
GGCGCACTGA CGCCCATTGT GGAGGGCCGC AGTACCATGG TAGCTAGCAG CAGCCATATG 
GGCAGATCAA TAACCCGAAA ATTGATGAAG GGCGTTAGCA TGGGTAATCT CAGATTCCCA 
TTCAGTACGC CAGAGACAAC CAAACGACTG GTGCGAAGTG TTTCTGCCAC GCTCAGGAGG 
CGCCCCAGTG GCGAAGAAAG CAAGCCATGC CCATTGGCAG CGGATGCACC ACTTCTGGAG 
GATGTAGACG ATAGCGATGA AGACGACGAC CATCATGGGG CGGTGGATGA GGACGATGAT 
GATACACTTT CGGAAAACAA TGGCAGCTCC AACGAGCTGC CCGCCATGAG CCTGGGCTAT 
CAGCAGATAC TACAGAGCAG CGTATATCGC AACATGGATC TCATTACGAG CACACCAGCT 
CTGCATTTGG GTCGCCGTTC CATGTCGCCC ATCACCAAGT CAACCCAACG CATGCCTAAG 
GCGATGCAGG AATCTATCAT GACTCCCCGA TCCCGAAAAC CGGTGATGTT GCTTACCGCC 
TTAGCTGGCA ATGGCGACAA GCAGCTAAAT CTGAGCTTCG CCGAGCAAGA TGAGCCGGAT 
TCGATTGGTG GCACGCCGAC CAAGCAACTG GAGCCACCAT CGCTGCACAG TGAGGATGCC 
ACATCACTGG GGGGATATGC GGATATCCTA GGGCAGCATC AGAGCTTTGC TCTCTTTAAT 
CCCAGAGAGC GTACGACCAG CAGTAGCAAC AGCAATGAAG ACTCAAAGCC CTCAAACGCC 
TTATCCAGTG ATTTCAAGGA ATATTTGCTA ACGCGCAGTG TTCTTACCGC CAGTCCGGCT 
GATTTATCAT TTGCCAGTCG GTCGGATGAT TTCGGTGGAG CCAGCACCAC GCAGGACATC 
GACGACTTGG ACGAGTCCGA TCTGAGCCCC AGTCTGCTCT ACTGCCTGGA CGGAAATGAG 
CCAACTTTAG CCTCACCCTT TGGCAACAGC AACGGCAGGA AGCGATCGGC GGGCACACCC 
CTAAAGTCTA CGCTAATTAC CAACCTTGAC GCCGACAACA AGGAAAACAT GGAGAATCTA 
CAGGAGGATC ATTCGCGCAC CAAAAAGCTG CTAATTACTT CTCCCGAGAA GTATCCGGGC 
GAAACGGCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261060.1
CDS	335..3349
Translation	MEATMDTDHANLNDFRDRDLATRMRRCNYEKYKSLVRMHLSFELELNTDEFDLPCHEIVYEDKGKLKKWNRLSKKHRLGHGAAATSCAAGSASKSVGNSPTETLQQQIDPGFLMHLQELKEFLMLEKNLTQEGLFRKAGAVSRQNELRMHIQHDKPLNLELAGFSAHDCATVFKGFLSELPEPLLTDAHYPAHLQIAPLCQALNGQTTATAERQQHLLNSVQLLLLLLPEEHRELLQHIIEMLHAVAKHEKSNKMSADNLATLFTPHLICPRQLPPEVLHYQAKKMSSIVTYMIVRGLDIFEVPGKLSTDIRAYFLERKRKKTMSPEQTLDESISDVSTVNTVYTFVDRAATAAATNTNNTDTELAQLYAHIQSMPESSKKRRLIKQFNKQNGQGTPLQLVVMNRLKNNEATRSAKSLGDSIKKHIFHKSLMSRTPKRVPPSFHLASGSETPNMSHVKPPKMRVLFQSPTPPTPTSSTITSVSLSTNTRHQLQKSISSASLKIESSSSDSSSSCATSGSISAPVSRQQSKESPSGNGAAGASGQELDEIDADCCVTPLKIMSAVAKQLIDKKSVAWDEHRLLEIEEYSNPSTPVQQSTRYKSEPNLSSILPQVDAEEEDDGALTPIVEGRSTMVASSSHMGRSITRKLMKGVSMGNLRFPFSTPETTKRLVRSVSATLRRRPSGEESKPCPLAADAPLLEDVDDSDEDDDHHGAVDEDDDDTLSENNGSSNELPAMSLGYQQILQSSVYRNMDLITSTPALHLGRRSMSPITKSTQRMPKAMQESIMTPRSRKPVMLLTALAGNGDKQLNLSFAEQDEPDSIGGTPTKQLEPPSLHSEDATSLGGYADILGQHQSFALFNPRERTTSSSNSNEDSKPSNALSSDFKEYLLTRSVLTASPADLSFASRSDDFGGASTTQDIDDLDESDLSPSLLYCLDGNEPTLASPFGNSNGRKRSAGTPLKSTLITNLDADNKENMENLQEDHSRTKKLLITSPEKYPGETAL

Target ORF information:

RefSeq Version	NM_001274131.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274131.2

ORF Insert Sequence:

ATGGAAGCAA CGATGGATAC GGACCATGCG AACCTAAATG ACTTCAGGGA TCGCGATCTG 
GCCACGCGCA TGCGCCGCTG CAATTACGAA AAATACAAAT CCTTGGTGCG CATGCACCTA 
TCGTTTGAAT TGGAGCTCAA TACGGATGAA TTCGATCTCC CCTGTCACGA GATTGTCTAC 
GAGGACAAGG GCAAGCTGAA GAAGTGGAAC CGCCTGTCTA AAAAACATCG TTTGGGACAT 
GGAGCAGCTG CCACATCCTG TGCCGCCGGC TCCGCCAGTA AATCCGTGGG TAATAGTCCC 
ACAGAGACAC TGCAACAGCA GATTGATCCG GGATTCTTAA TGCATCTGCA AGAGCTCAAG 
GAGTTCCTAA TGCTGGAGAA GAATCTCACC CAGGAAGGCC TATTCCGCAA GGCTGGTGCG 
GTGTCCCGAC AAAACGAACT TCGGATGCAC ATCCAGCACG ACAAACCACT GAATTTGGAA 
CTTGCGGGCT TCTCCGCTCA CGATTGCGCG ACCGTTTTCA AAGGTTTTCT ATCCGAACTT 
CCAGAACCGC TGCTTACGGA TGCCCACTAT CCGGCGCATC TGCAGATAGC CCCACTCTGC 
CAGGCGCTCA ATGGTCAGAC GACTGCCACT GCTGAGCGTC AACAACATCT TCTCAACTCC 
GTGCAGCTGC TACTTCTCCT CCTGCCCGAA GAACATCGTG AACTTCTTCA GCACATCATT 
GAGATGCTGC ATGCTGTGGC CAAGCATGAG AAAAGCAACA AGATGAGCGC CGATAACTTG 
GCCACTCTCT TCACACCGCA CCTAATTTGC CCAAGGCAAC TGCCACCCGA GGTCCTGCAC 
TACCAGGCCA AGAAAATGTC CAGCATCGTG ACCTATATGA TCGTCCGTGG ACTGGACATC 
TTCGAGGTGC CTGGAAAGCT GTCTACCGAC ATCCGGGCGT ACTTTCTCGA GCGCAAAAGA 
AAGAAAACCA TGTCGCCGGA ACAAACGCTT GACGAGTCCA TCTCGGACGT CTCGACGGTC 
AACACGGTGT ACACTTTTGT AGACCGAGCC GCCACTGCAG CGGCCACCAA TACGAACAAT 
ACGGACACGG AGCTGGCGCA ACTTTATGCC CATATCCAGA GCATGCCGGA GTCCTCTAAG 
AAGAGGCGTT TGATTAAGCA GTTCAACAAG CAAAATGGGC AGGGCACACC GTTGCAACTG 
GTGGTAATGA ATCGTTTGAA GAACAACGAG GCCACTCGAA GTGCCAAGTC CCTGGGAGAT 
TCCATTAAGA AGCACATTTT CCACAAGAGC CTCATGTCGC GGACTCCAAA AAGAGTGCCA 
CCCAGTTTTC ATTTGGCTAG TGGATCCGAG ACGCCCAATA TGTCGCATGT GAAACCGCCC 
AAGATGCGGG TGCTTTTTCA GAGCCCCACG CCACCCACAC CCACCTCATC GACCATCACC 
AGCGTCAGCC TGTCCACTAA CACTCGGCAT CAGCTTCAGA AATCCATCTC ATCTGCTTCC 
CTCAAGATCG AATCCTCGTC TTCGGACAGC AGCTCTAGCT GTGCGACCAG CGGTAGTATC 
TCTGCTCCAG TCAGTCGACA GCAATCGAAA GAATCGCCTT CTGGCAATGG AGCTGCTGGT 
GCTTCCGGCC AGGAACTGGA TGAGATCGAT GCCGATTGCT GCGTGACGCC TTTAAAGATC 
ATGTCAGCGG TTGCAAAGCA GCTAATTGAC AAGAAGAGCG TTGCCTGGGA CGAGCACCGA 
CTGCTGGAGA TTGAGGAGTA CTCGAATCCC TCAACGCCCG TTCAGCAATC GACGCGCTAT 
AAGTCGGAGC CTAATCTGTC AAGTATCCTG CCGCAGGTGG ACGCAGAGGA AGAGGACGAC 
GGCGCACTGA CGCCCATTGT GGAGGGCCGC AGTACCATGG TAGCTAGCAG CAGCCATATG 
GGCAGATCAA TAACCCGAAA ATTGATGAAG GGCGTTAGCA TGGGTAATCT CAGATTCCCA 
TTCAGTACGC CAGAGACAAC CAAACGACTG GTGCGAAGTG TTTCTGCCAC GCTCAGGAGG 
CGCCCCAGTG GCGAAGAAAG CAAGCCATGC CCATTGGCAG CGGATGCACC ACTTCTGGAG 
GATGTAGACG ATAGCGATGA AGACGACGAC CATCATGGGG CGGTGGATGA GGACGATGAT 
GATACACTTT CGGAAAACAA TGGCAGCTCC AACGAGCTGC CCGCCATGAG CCTGGGCTAT 
CAGCAGATAC TACAGAGCAG CGTATATCGC AACATGGATC TCATTACGAG CACACCAGCT 
CTGCATTTGG GTCGCCGTTC CATGTCGCCC ATCACCAAGT CAACCCAACG CATGCCTAAG 
GCGATGCAGG AATCTATCAT GACTCCCCGA TCCCGAAAAC CGGTGATGTT GCTTACCGCC 
TTAGCTGGCA ATGGCGACAA GCAGCTAAAT CTGAGCTTCG CCGAGCAAGA TGAGCCGGAT 
TCGATTGGTG GCACGCCGAC CAAGCAACTG GAGCCACCAT CGCTGCACAG TGAGGATGCC 
ACATCACTGG GGGGATATGC GGATATCCTA GGGCAGCATC AGAGCTTTGC TCTCTTTAAT 
CCCAGAGAGC GTACGACCAG CAGTAGCAAC AGCAATGAAG ACTCAAAGCC CTCAAACGCC 
TTATCCAGTG ATTTCAAGGA ATATTTGCTA ACGCGCAGTG TTCTTACCGC CAGTCCGGCT 
GATTTATCAT TTGCCAGTCG GTCGGATGAT TTCGGTGGAG CCAGCACCAC GCAGGACATC 
GACGACTTGG ACGAGTCCGA TCTGAGCCCC AGTCTGCTCT ACTGCCTGGA CGGAAATGAG 
CCAACTTTAG CCTCACCCTT TGGCAACAGC AACGGCAGGA AGCGATCGGC GGGCACACCC 
CTAAAGTCTA CGCTAATTAC CAACCTTGAC GCCGACAACA AGGAAAACAT GGAGAATCTA 
CAGGAGGATC ATTCGCGCAC CAAAAAGCTG CTAATTACTT CTCCCGAGAA GTATCCGGGC 
GAAACGGCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP54D ( NM_137392.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611236.2 Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10360
Clone ID Related Accession (Same CDS sequence)	NM_001274131.2 , NM_137392.3
Accession Version	NM_137392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 54D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137392.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGCAA CGATGGATAC GGACCATGCG AACCTAAATG ACTTCAGGGA TCGCGATCTG 
GCCACGCGCA TGCGCCGCTG CAATTACGAA AAATACAAAT CCTTGGTGCG CATGCACCTA 
TCGTTTGAAT TGGAGCTCAA TACGGATGAA TTCGATCTCC CCTGTCACGA GATTGTCTAC 
GAGGACAAGG GCAAGCTGAA GAAGTGGAAC CGCCTGTCTA AAAAACATCG TTTGGGACAT 
GGAGCAGCTG CCACATCCTG TGCCGCCGGC TCCGCCAGTA AATCCGTGGG TAATAGTCCC 
ACAGAGACAC TGCAACAGCA GATTGATCCG GGATTCTTAA TGCATCTGCA AGAGCTCAAG 
GAGTTCCTAA TGCTGGAGAA GAATCTCACC CAGGAAGGCC TATTCCGCAA GGCTGGTGCG 
GTGTCCCGAC AAAACGAACT TCGGATGCAC ATCCAGCACG ACAAACCACT GAATTTGGAA 
CTTGCGGGCT TCTCCGCTCA CGATTGCGCG ACCGTTTTCA AAGGTTTTCT ATCCGAACTT 
CCAGAACCGC TGCTTACGGA TGCCCACTAT CCGGCGCATC TGCAGATAGC CCCACTCTGC 
CAGGCGCTCA ATGGTCAGAC GACTGCCACT GCTGAGCGTC AACAACATCT TCTCAACTCC 
GTGCAGCTGC TACTTCTCCT CCTGCCCGAA GAACATCGTG AACTTCTTCA GCACATCATT 
GAGATGCTGC ATGCTGTGGC CAAGCATGAG AAAAGCAACA AGATGAGCGC CGATAACTTG 
GCCACTCTCT TCACACCGCA CCTAATTTGC CCAAGGCAAC TGCCACCCGA GGTCCTGCAC 
TACCAGGCCA AGAAAATGTC CAGCATCGTG ACCTATATGA TCGTCCGTGG ACTGGACATC 
TTCGAGGTGC CTGGAAAGCT GTCTACCGAC ATCCGGGCGT ACTTTCTCGA GCGCAAAAGA 
AAGAAAACCA TGTCGCCGGA ACAAACGCTT GACGAGTCCA TCTCGGACGT CTCGACGGTC 
AACACGGTGT ACACTTTTGT AGACCGAGCC GCCACTGCAG CGGCCACCAA TACGAACAAT 
ACGGACACGG AGCTGGCGCA ACTTTATGCC CATATCCAGA GCATGCCGGA GTCCTCTAAG 
AAGAGGCGTT TGATTAAGCA GTTCAACAAG CAAAATGGGC AGGGCACACC GTTGCAACTG 
GTGGTAATGA ATCGTTTGAA GAACAACGAG GCCACTCGAA GTGCCAAGTC CCTGGGAGAT 
TCCATTAAGA AGCACATTTT CCACAAGAGC CTCATGTCGC GGACTCCAAA AAGAGTGCCA 
CCCAGTTTTC ATTTGGCTAG TGGATCCGAG ACGCCCAATA TGTCGCATGT GAAACCGCCC 
AAGATGCGGG TGCTTTTTCA GAGCCCCACG CCACCCACAC CCACCTCATC GACCATCACC 
AGCGTCAGCC TGTCCACTAA CACTCGGCAT CAGCTTCAGA AATCCATCTC ATCTGCTTCC 
CTCAAGATCG AATCCTCGTC TTCGGACAGC AGCTCTAGCT GTGCGACCAG CGGTAGTATC 
TCTGCTCCAG TCAGTCGACA GCAATCGAAA GAATCGCCTT CTGGCAATGG AGCTGCTGGT 
GCTTCCGGCC AGGAACTGGA TGAGATCGAT GCCGATTGCT GCGTGACGCC TTTAAAGATC 
ATGTCAGCGG TTGCAAAGCA GCTAATTGAC AAGAAGAGCG TTGCCTGGGA CGAGCACCGA 
CTGCTGGAGA TTGAGGAGTA CTCGAATCCC TCAACGCCCG TTCAGCAATC GACGCGCTAT 
AAGTCGGAGC CTAATCTGTC AAGTATCCTG CCGCAGGTGG ACGCAGAGGA AGAGGACGAC 
GGCGCACTGA CGCCCATTGT GGAGGGCCGC AGTACCATGG TAGCTAGCAG CAGCCATATG 
GGCAGATCAA TAACCCGAAA ATTGATGAAG GGCGTTAGCA TGGGTAATCT CAGATTCCCA 
TTCAGTACGC CAGAGACAAC CAAACGACTG GTGCGAAGTG TTTCTGCCAC GCTCAGGAGG 
CGCCCCAGTG GCGAAGAAAG CAAGCCATGC CCATTGGCAG CGGATGCACC ACTTCTGGAG 
GATGTAGACG ATAGCGATGA AGACGACGAC CATCATGGGG CGGTGGATGA GGACGATGAT 
GATACACTTT CGGAAAACAA TGGCAGCTCC AACGAGCTGC CCGCCATGAG CCTGGGCTAT 
CAGCAGATAC TACAGAGCAG CGTATATCGC AACATGGATC TCATTACGAG CACACCAGCT 
CTGCATTTGG GTCGCCGTTC CATGTCGCCC ATCACCAAGT CAACCCAACG CATGCCTAAG 
GCGATGCAGG AATCTATCAT GACTCCCCGA TCCCGAAAAC CGGTGATGTT GCTTACCGCC 
TTAGCTGGCA ATGGCGACAA GCAGCTAAAT CTGAGCTTCG CCGAGCAAGA TGAGCCGGAT 
TCGATTGGTG GCACGCCGAC CAAGCAACTG GAGCCACCAT CGCTGCACAG TGAGGATGCC 
ACATCACTGG GGGGATATGC GGATATCCTA GGGCAGCATC AGAGCTTTGC TCTCTTTAAT 
CCCAGAGAGC GTACGACCAG CAGTAGCAAC AGCAATGAAG ACTCAAAGCC CTCAAACGCC 
TTATCCAGTG ATTTCAAGGA ATATTTGCTA ACGCGCAGTG TTCTTACCGC CAGTCCGGCT 
GATTTATCAT TTGCCAGTCG GTCGGATGAT TTCGGTGGAG CCAGCACCAC GCAGGACATC 
GACGACTTGG ACGAGTCCGA TCTGAGCCCC AGTCTGCTCT ACTGCCTGGA CGGAAATGAG 
CCAACTTTAG CCTCACCCTT TGGCAACAGC AACGGCAGGA AGCGATCGGC GGGCACACCC 
CTAAAGTCTA CGCTAATTAC CAACCTTGAC GCCGACAACA AGGAAAACAT GGAGAATCTA 
CAGGAGGATC ATTCGCGCAC CAAAAAGCTG CTAATTACTT CTCCCGAGAA GTATCCGGGC 
GAAACGGCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611236.2
CDS	531..3545
Translation	MEATMDTDHANLNDFRDRDLATRMRRCNYEKYKSLVRMHLSFELELNTDEFDLPCHEIVYEDKGKLKKWNRLSKKHRLGHGAAATSCAAGSASKSVGNSPTETLQQQIDPGFLMHLQELKEFLMLEKNLTQEGLFRKAGAVSRQNELRMHIQHDKPLNLELAGFSAHDCATVFKGFLSELPEPLLTDAHYPAHLQIAPLCQALNGQTTATAERQQHLLNSVQLLLLLLPEEHRELLQHIIEMLHAVAKHEKSNKMSADNLATLFTPHLICPRQLPPEVLHYQAKKMSSIVTYMIVRGLDIFEVPGKLSTDIRAYFLERKRKKTMSPEQTLDESISDVSTVNTVYTFVDRAATAAATNTNNTDTELAQLYAHIQSMPESSKKRRLIKQFNKQNGQGTPLQLVVMNRLKNNEATRSAKSLGDSIKKHIFHKSLMSRTPKRVPPSFHLASGSETPNMSHVKPPKMRVLFQSPTPPTPTSSTITSVSLSTNTRHQLQKSISSASLKIESSSSDSSSSCATSGSISAPVSRQQSKESPSGNGAAGASGQELDEIDADCCVTPLKIMSAVAKQLIDKKSVAWDEHRLLEIEEYSNPSTPVQQSTRYKSEPNLSSILPQVDAEEEDDGALTPIVEGRSTMVASSSHMGRSITRKLMKGVSMGNLRFPFSTPETTKRLVRSVSATLRRRPSGEESKPCPLAADAPLLEDVDDSDEDDDHHGAVDEDDDDTLSENNGSSNELPAMSLGYQQILQSSVYRNMDLITSTPALHLGRRSMSPITKSTQRMPKAMQESIMTPRSRKPVMLLTALAGNGDKQLNLSFAEQDEPDSIGGTPTKQLEPPSLHSEDATSLGGYADILGQHQSFALFNPRERTTSSSNSNEDSKPSNALSSDFKEYLLTRSVLTASPADLSFASRSDDFGGASTTQDIDDLDESDLSPSLLYCLDGNEPTLASPFGNSNGRKRSAGTPLKSTLITNLDADNKENMENLQEDHSRTKKLLITSPEKYPGETAL

Target ORF information:

RefSeq Version	NM_137392.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 54D (RhoGAP54D), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137392.3

ORF Insert Sequence:

ATGGAAGCAA CGATGGATAC GGACCATGCG AACCTAAATG ACTTCAGGGA TCGCGATCTG 
GCCACGCGCA TGCGCCGCTG CAATTACGAA AAATACAAAT CCTTGGTGCG CATGCACCTA 
TCGTTTGAAT TGGAGCTCAA TACGGATGAA TTCGATCTCC CCTGTCACGA GATTGTCTAC 
GAGGACAAGG GCAAGCTGAA GAAGTGGAAC CGCCTGTCTA AAAAACATCG TTTGGGACAT 
GGAGCAGCTG CCACATCCTG TGCCGCCGGC TCCGCCAGTA AATCCGTGGG TAATAGTCCC 
ACAGAGACAC TGCAACAGCA GATTGATCCG GGATTCTTAA TGCATCTGCA AGAGCTCAAG 
GAGTTCCTAA TGCTGGAGAA GAATCTCACC CAGGAAGGCC TATTCCGCAA GGCTGGTGCG 
GTGTCCCGAC AAAACGAACT TCGGATGCAC ATCCAGCACG ACAAACCACT GAATTTGGAA 
CTTGCGGGCT TCTCCGCTCA CGATTGCGCG ACCGTTTTCA AAGGTTTTCT ATCCGAACTT 
CCAGAACCGC TGCTTACGGA TGCCCACTAT CCGGCGCATC TGCAGATAGC CCCACTCTGC 
CAGGCGCTCA ATGGTCAGAC GACTGCCACT GCTGAGCGTC AACAACATCT TCTCAACTCC 
GTGCAGCTGC TACTTCTCCT CCTGCCCGAA GAACATCGTG AACTTCTTCA GCACATCATT 
GAGATGCTGC ATGCTGTGGC CAAGCATGAG AAAAGCAACA AGATGAGCGC CGATAACTTG 
GCCACTCTCT TCACACCGCA CCTAATTTGC CCAAGGCAAC TGCCACCCGA GGTCCTGCAC 
TACCAGGCCA AGAAAATGTC CAGCATCGTG ACCTATATGA TCGTCCGTGG ACTGGACATC 
TTCGAGGTGC CTGGAAAGCT GTCTACCGAC ATCCGGGCGT ACTTTCTCGA GCGCAAAAGA 
AAGAAAACCA TGTCGCCGGA ACAAACGCTT GACGAGTCCA TCTCGGACGT CTCGACGGTC 
AACACGGTGT ACACTTTTGT AGACCGAGCC GCCACTGCAG CGGCCACCAA TACGAACAAT 
ACGGACACGG AGCTGGCGCA ACTTTATGCC CATATCCAGA GCATGCCGGA GTCCTCTAAG 
AAGAGGCGTT TGATTAAGCA GTTCAACAAG CAAAATGGGC AGGGCACACC GTTGCAACTG 
GTGGTAATGA ATCGTTTGAA GAACAACGAG GCCACTCGAA GTGCCAAGTC CCTGGGAGAT 
TCCATTAAGA AGCACATTTT CCACAAGAGC CTCATGTCGC GGACTCCAAA AAGAGTGCCA 
CCCAGTTTTC ATTTGGCTAG TGGATCCGAG ACGCCCAATA TGTCGCATGT GAAACCGCCC 
AAGATGCGGG TGCTTTTTCA GAGCCCCACG CCACCCACAC CCACCTCATC GACCATCACC 
AGCGTCAGCC TGTCCACTAA CACTCGGCAT CAGCTTCAGA AATCCATCTC ATCTGCTTCC 
CTCAAGATCG AATCCTCGTC TTCGGACAGC AGCTCTAGCT GTGCGACCAG CGGTAGTATC 
TCTGCTCCAG TCAGTCGACA GCAATCGAAA GAATCGCCTT CTGGCAATGG AGCTGCTGGT 
GCTTCCGGCC AGGAACTGGA TGAGATCGAT GCCGATTGCT GCGTGACGCC TTTAAAGATC 
ATGTCAGCGG TTGCAAAGCA GCTAATTGAC AAGAAGAGCG TTGCCTGGGA CGAGCACCGA 
CTGCTGGAGA TTGAGGAGTA CTCGAATCCC TCAACGCCCG TTCAGCAATC GACGCGCTAT 
AAGTCGGAGC CTAATCTGTC AAGTATCCTG CCGCAGGTGG ACGCAGAGGA AGAGGACGAC 
GGCGCACTGA CGCCCATTGT GGAGGGCCGC AGTACCATGG TAGCTAGCAG CAGCCATATG 
GGCAGATCAA TAACCCGAAA ATTGATGAAG GGCGTTAGCA TGGGTAATCT CAGATTCCCA 
TTCAGTACGC CAGAGACAAC CAAACGACTG GTGCGAAGTG TTTCTGCCAC GCTCAGGAGG 
CGCCCCAGTG GCGAAGAAAG CAAGCCATGC CCATTGGCAG CGGATGCACC ACTTCTGGAG 
GATGTAGACG ATAGCGATGA AGACGACGAC CATCATGGGG CGGTGGATGA GGACGATGAT 
GATACACTTT CGGAAAACAA TGGCAGCTCC AACGAGCTGC CCGCCATGAG CCTGGGCTAT 
CAGCAGATAC TACAGAGCAG CGTATATCGC AACATGGATC TCATTACGAG CACACCAGCT 
CTGCATTTGG GTCGCCGTTC CATGTCGCCC ATCACCAAGT CAACCCAACG CATGCCTAAG 
GCGATGCAGG AATCTATCAT GACTCCCCGA TCCCGAAAAC CGGTGATGTT GCTTACCGCC 
TTAGCTGGCA ATGGCGACAA GCAGCTAAAT CTGAGCTTCG CCGAGCAAGA TGAGCCGGAT 
TCGATTGGTG GCACGCCGAC CAAGCAACTG GAGCCACCAT CGCTGCACAG TGAGGATGCC 
ACATCACTGG GGGGATATGC GGATATCCTA GGGCAGCATC AGAGCTTTGC TCTCTTTAAT 
CCCAGAGAGC GTACGACCAG CAGTAGCAAC AGCAATGAAG ACTCAAAGCC CTCAAACGCC 
TTATCCAGTG ATTTCAAGGA ATATTTGCTA ACGCGCAGTG TTCTTACCGC CAGTCCGGCT 
GATTTATCAT TTGCCAGTCG GTCGGATGAT TTCGGTGGAG CCAGCACCAC GCAGGACATC 
GACGACTTGG ACGAGTCCGA TCTGAGCCCC AGTCTGCTCT ACTGCCTGGA CGGAAATGAG 
CCAACTTTAG CCTCACCCTT TGGCAACAGC AACGGCAGGA AGCGATCGGC GGGCACACCC 
CTAAAGTCTA CGCTAATTAC CAACCTTGAC GCCGACAACA AGGAAAACAT GGAGAATCTA 
CAGGAGGATC ATTCGCGCAC CAAAAAGCTG CTAATTACTT CTCCCGAGAA GTATCCGGGC 
GAAACGGCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP54D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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