Dcr-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcr-2
Entrez Gene ID	36993
Full Name	Dicer-2
Synonyms	CG6493,DCR-2,DCR2,DICER,Dcr,Dcr2,Dicer,Dicer2,Dmel\CG6493,cg6493,dcr,dcr-2,dcr2,dic2,dicer,dicer-2,dicer2,dmDcr-2
General protein information	Preferred Names Dicer-2 Names CG6493 gene product from transcript CG6493-RB CG6493-PA CG6493-PB Dcr-2-PA Dcr-2-PB Dicer2 dicer dicer 2 dicer-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54C10-54C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299611.1	NP_001286540.1	Dicer-2, isoform B
NM_079054.5	NP_523778.2	Dicer-2, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012289	XP_320248.4
Arabidopsis thaliana (thale cress)	DCL2	NP_001189798.1
Oryza sativa (rice)	Os03g0583900	NP_001050564.1
Drosophila melanogaster (fruit fly)	Dcr-2	NP_523778.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

binding preference for the thermodynamically more stable siRNA end by Loqs-PD alone is greatly amplified in complex with Dcr-2 to initiate strand discrimination by asymmetry sensing in the RISC loading complex.
Title: Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.

Study found that dsRNA-binding domain of Dicer-2 is crucial for efficient and high-fidelity 21-nt siRNA production and for siRNA loading to Argonaute2.
Title: The C-terminal dsRNA-binding domain of

Partial reconstitution of the RNAi response in human cells has been demonstrated using Drosophila gene products, DCR-2, Loqs-PD, and R2D2.
Title: Partial reconstitution of the RNAi response in human cells using Drosophila gene products.

Data show that dmDcr-2's partner protein, Loquacious-PD, alters termini dependence, enabling dmDcr-2 to cleave substrates normally refractory to cleavage.
Title: Drosophila dicer-2 cleavage is mediated by helicase- and dsRNA termini-dependent states that are modulated by Loquacious-PD.

Inorganic phosphate occupies the phosphate-binding pocket in the fly Dicer-2, blocking binding of pre-miRNA and restricting pre-miRNA processing to Dicer-1.
Title: A universal small molecule, inorganic phosphate, restricts the substrate specificity of Dicer-2 in small RNA biogenesis.

The following Dcr-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcr-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13066	NM_079054.5 Latest version!	Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21845	NM_001299611.1 Latest version!	Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcr-2 ( NM_079054.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523778.2 Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13066
Clone ID Related Accession (Same CDS sequence)	NM_079054.5
Accession Version	NM_079054.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dicer-2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079054.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGATG TGGAAATCAA GCCTCGCGGC TATCAACTTC GCCTGGTTGA CCACCTAACG 
AAGAGCAATG GCATTGTCTA CCTGCCCACA GGATCTGGGA AAACGTTCGT GGCCATCCTG 
GTCCTAAAGC GCTTCTCGCA GGACTTTGAC AAACCCATCG AAAGCGGCGG AAAGCGTGCG 
TTGTTCATGT GCAATACTGT GGAATTGGCC CGACAACAGG CGATGGCCGT CAGGCGGTGC 
ACCAACTTTA AGGTTGGCTT TTATGTGGGT GAACAGGGAG TGGATGACTG GACGCGCGGA 
ATGTGGAGTG ATGAAATCAA AAAAAACCAA GTTCTTGTTG GCACAGCCCA GGTCTTTTTG 
GACATGGTCA CCCAGACGTA CGTGGCTTTA AGCTCCCTGA GCGTTGTCAT CATAGACGAG 
TGCCACCACG GCACTGGTCA CCACCCATTT CGTGAGTTCA TGCGATTGTT CACTATCGCT 
AATCAAACAA AATTGCCGCG CGTGGTGGGT CTCACCGGGG TGTTAATCAA GGGAAATGAA 
ATTACGAACG TGGCCACGAA ACTTAAAGAG CTAGAGATCA CATATCGGGG AAACATCATT 
ACCGTGTCCG ACACCAAGGA GATGGAAAAC GTGATGCTGT ACGCCACCAA GCCCACAGAG 
GTCATGGTGT CCTTTCCACA TCAAGAGCAA GTGCTCACGG TTACAAGGTT AATAAGCGCA 
GAAATTGAAA AGTTCTATGT ATCCTTAGAC CTCATGAATA TTGGCGTTCA ACCGATCCGA 
AGGTCAAAGT CATTGCAATG CCTGCGGGAT CCGTCGAAGA AGAGCTTTGT AAAGCAACTG 
TTCAACGATT TTCTGTACCA GATGAAAGAG TATGGCATCT ACGCAGCTTC CATAGCCATA 
ATCTCGTTAA TTGTTGAGTT CGATATAAAG AGGCGCCAGG CCGAGACGCT AAGCGTGAAA 
TTGATGCATA GGACGGCCTT GACCTTGTGC GAGAAAATCC GTCACTTGCT GGTGCAAAAA 
CTGCAAGACA TGACTTATGA CGACGACGAT GACAACGTAA ACACTGAGGA AGTCATTATG 
AACTTCTCCA CCCCGAAAGT GCAGCGATTC CTGATGAGTC TTAAGGTATC CTTCGCTGAT 
AAAGATCCCA AAGATATCTG CTGTCTGGTC TTTGTTGAAC GCCGCTACAC CTGCAAGTGC 
ATCTATGGCT TGCTGCTCAA CTACATCCAG TCTACGCCTG AGCTGCGAAA CGTGCTCACG 
CCCCAATTCA TGGTGGGCCG CAACAATATT TCTCCAGACT TTGAGAGCGT CTTGGAGAGA 
AAATGGCAAA AATCTGCCAT TCAGCAGTTT CGGGACGGTA ATGCCAATCT TATGATATGT 
TCAAGCGTCC TAGAAGAAGG CATCGATGTG CAGGCCTGCA ATCATGTCTT TATTCTTGAT 
CCTGTTAAAA CCTTTAACAT GTACGTGCAG TCAAAGGGTC GGGCTCGTAC CACTGAGGCC 
AAATTCGTGC TGTTCACAGC TGATAAGGAA AGGGAAAAGA CAATTCAGCA AATTTATCAG 
TATCGAAAAG CGCATAATGA CATTGCCGAA TATTTAAAGG ATCGAGTTCT AGAAAAAACT 
GAGCCAGAAT TGTACGAAAT CAAAGGGCAC TTCCAGGACG ATATTGACCC TTTTACCAAT 
GAGAACGGTG CCGTGTTGCT TCCCAACAAT GCCCTGGCCA TACTGCATCG CTATTGCCAG 
ACAATTCCCA CGGATGCTTT TGGCTTTGTG ATCCCCTGGT TCCATGTATT GCAAGAAGAC 
GAACGTGATA GAATATTTGG TGTTTCTGCC AAGGGCAAGC ACGTAATTTC AATCAACATG 
CCAGTAAATT GCATGCTAAG AGACACAATT TACAGCGATC CCATGGACAA TGTTAAAACA 
GCCAAAATAT CAGCTGCCTT CAAAGCGTGT AAAGTACTCT ACAGTTTGGG AGAGTTAAAC 
GAGAGATTCG TGCCCAAAAC ATTAAAGGAG CGCGTAGCCT CTATTGCTGA TGTTCATTTT 
GAGCACTGGA ATAAGTATGG CGATAGTGTG ACTGCGACAG TCAACAAGGC CGATAAGAGC 
AAGGACAGAA CCTACAAGAC CGAATGTCCA TTAGAGTTTT ACGACGCACT TCCGCGGGTA 
GGTGAAATCT GTTATGCCTA CGAAATATTT TTGGAGCCGC AGTTTGAATC GTGTGAGTAC 
ACGGAACACA TGTATTTAAA TCTCCAAACA CCAAGAAATT ATGCTATATT GCTGCGGAAC 
AAGCTGCCAC GCCTTGCAGA GATGCCCCTG TTTAGCAATC AGGGTAAGCT ACACGTTCGA 
GTGGCCAATG CACCACTTGA AGTAATTATC CAGAACTCCG AACAGCTGGA GCTGCTCCAT 
CAGTTTCACG GAATGGTATT CCGCGATATC TTGAAGATTT GGCATCCGTT CTTTGTGCTG 
GATCGTCGCA GCAAGGAGAA CTCCTACCTG GTAGTTCCTC TCATACTGGG TGCGGGTGAA 
CAGAAATGCT TTGACTGGGA ACTGATGACC AACTTCCGAA GACTACCGCA AAGTCATGGG 
TCAAATGTAC AGCAACGCGA GCAACAACCT GCTCCACGTC CTGAAGATTT TGAGGGCAAA 
ATTGTTACAC AATGGTATGC CAACTATGAT AAGCCCATGC TGGTCACAAA GGTGCACCGT 
GAGCTAACAC CGTTAAGTTA TATGGAAAAG AACCAACAGG ACAAGACCTA CTACGAGTTT 
ACCATGTCAA AGTACGGCAA CCGCATTGGT GATGTTGTTC ACAAGGATAA GTTTATGATC 
GAGGTCAGAG ACCTTACGGA ACAGCTCACT TTCTACGTTC ATAATCGTGG AAAGTTCAAC 
GCGAAGAGCA AGGCTAAGAT GAAAGTTATT TTGATTCCGG AGCTATGCTT CAATTTTAAC 
TTTCCTGGGG ATTTATGGCT TAAGTTGATC TTCCTACCCA GCATTTTAAA CCGCATGTAC 
TTCCTTCTCC ACGCAGAGGC CTTACGTAAG CGATTTAATA CGTATTTAAA CCTCCATCTG 
CTGCCTTTTA ATGGAACTGA TTACATGCCC AGACCACTAG AAATTGATTA TTCGCTAAAG 
CGGAATGTCG ACCCCTTGGG CAATGTCATA CCAACTGAGG ATATCGAGGA GCCGAAATCC 
CTTTTAGAGC CAATGCCCAC AAAGTCCATT GAGGCGTCCG TGGCCAATCT TGAAATAACA 
GAATTCGAAA ATCCCTGGCA AAAGTATATG GAGCCGGTTG ATCTGTCGCG AAATCTTTTG 
AGTACGTATC CCGTAGAGCT GGACTACTAC TATCATTTTA GCGTTGGTAA TGTATGTGAG 
ATGAATGAGA TGGATTTTGA AGATAAGGAA TACTGGGCAA AAAATCAGTT CCATATGCCC 
ACGGGTAATA TCTATGGCAA CAGAACTCCG GCCAAGACTA ACGCAAACGT GCCAGCTTTG 
ATGCCGTCGA AACCGACTGT CCGCGGGAAG GTAAAACCAC TGCTCATTTT GCAAAAAACT 
GTTTCCAAAG AACATATAAC GCCAGCGGAG CAGGGCGAGT TTTTGGCTGC AATCACTGCC 
TCGAGCGCAG CGGATGTTTT CGACATGGAA CGCCTTGAGA TTCTGGGCGA TTCCTTCTTA 
AAACTTAGTG CCACGCTCTA CTTGGCCAGC AAGTATTCGG ATTGGAATGA GGGCACTCTT 
ACAGAGGTCA AATCCAAGCT TGTGTCTAAT CGTAACCTGT TGTTCTGCCT CATCGACGCG 
GACATTCCCA AAACGCTCAA CACCATTCAG TTTACGCCTA GATACACATG GCTTCCACCT 
GGCATCAGTT TGCCCCACAA TGTTCTAGCC TTGTGGCGAG AAAATCCGGA ATTCGCAAAG 
ATTATTGGCC CACACAATCT CCGTGACTTG GCTCTCGGCG ATGAGGAGTC CCTGGTGAAG 
GGCAACTGCA GCGATATCAA CTACAACCGC TTCGTGGAGG GATGTCGAGC AAATGGGCAG 
TCCTTTTATG CCGGTGCCGA TTTTTCATCC GAGGTGAACT TCTGCGTGGG ACTGGTCACG 
ATTCCCAACA AGGTGATAGC CGATACCCTT GAGGCGCTAT TGGGCGTAAT CGTTAAGAAC 
TACGGATTGC AGCATGCCTT CAAGATGCTA GAGTACTTCA AGATTTGCCG TGCGGATATT 
GACAAGCCGC TTACTCAGTT ACTGAACCTC GAACTGGGCG GCAAGAAAAT GCGGGCGAAC 
GTGAATACTA CAGAAATTGA CGGCTTTCTC ATAAATCACT ATTATTTGGA AAAAAATCTT 
GGCTACACGT TTAAGGATCG ACGATATTTG TTGCAGGCCC TCACCCATCC CTCGTATCCC 
ACTAATCGCA TCACTGGGTC CTATCAGGAG CTGGAATTTA TTGGCGATGC CATACTCGAC 
TTTCTAATCT CTGCCTATAT TTTCGAGAAC AATACGAAAA TGAATCCGGG CGCCCTCACA 
GATCTGCGTT CCGCTTTGGT CAACAATACT ACGCTGGCCT GCATTTGCGT GCGGCATCGG 
TTGCACTTTT TTATCCTAGC GGAGAACGCA AAGCTCTCCG AGATCATATC CAAATTCGTG 
AATTTCCAGG AGAGCCAGGG TCACCGGGTT ACCAACTATG TGCGCATTCT GCTCGAGGAA 
GCTGATGTTC AGCCGACTCC GCTGGACTTG GATGACGAAT TGGATATGAC TGAGTTACCG 
CATGCAAATA AATGCATTTC TCAGGAAGCA GAGAAAGGAG TCCCACCAAA AGGTGAGTTC 
AACATGTCCA CAAACGTGGA TGTGCCAAAG GCGTTGGGGG ATGTTCTGGA GGCCCTTATT 
GCAGCCGTAT ACCTGGACTG CCGAGATCTC CAACGTACTT GGGAGGTAAT TTTCAATCTC 
TTCGAGCCGG AGCTTCAGGA GTTCACGCGC AAAGTGCCCA TCAATCATAT ACGACAGCTC 
GTTGAGCACA AGCATGCGAA GCCCGTGTTT AGCTCGCCCA TCGTCGAAGG GGAAACTGTA 
ATGGTTAGTT GTCAATTTAC CTGCATGGAA AAGACGATCA AGGTGTACGG ATTTGGGAGC 
AATAAGGACC AGGCCAAGCT ATCGGCTGCC AAGCACGCCC TTCAGCAGCT AAGCAAATGC 
GACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523778.2
CDS	100..5268
Translation	MEDVEIKPRGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRFSQDFDKPIESGGKRALFMCNTVELARQQAMAVRRCTNFKVGFYVGEQGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVIIDECHHGTGHHPFREFMRLFTIANQTKLPRVVGLTGVLIKGNEITNVATKLKELEITYRGNIITVSDTKEMENVMLYATKPTEVMVSFPHQEQVLTVTRLISAEIEKFYVSLDLMNIGVQPIRRSKSLQCLRDPSKKSFVKQLFNDFLYQMKEYGIYAASIAIISLIVEFDIKRRQAETLSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEEVIMNFSTPKVQRFLMSLKVSFADKDPKDICCLVFVERRYTCKCIYGLLLNYIQSTPELRNVLTPQFMVGRNNISPDFESVLERKWQKSAIQQFRDGNANLMICSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFVLFTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLEKTEPELYEIKGHFQDDIDPFTNENGAVLLPNNALAILHRYCQTIPTDAFGFVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCMLRDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVPKTLKERVASIADVHFEHWNKYGDSVTATVNKADKSKDRTYKTECPLEFYDALPRVGEICYAYEIFLEPQFESCEYTEHMYLNLQTPRNYAILLRNKLPRLAEMPLFSNQGKLHVRVANAPLEVIIQNSEQLELLHQFHGMVFRDILKIWHPFFVLDRRSKENSYLVVPLILGAGEQKCFDWELMTNFRRLPQSHGSNVQQREQQPAPRPEDFEGKIVTQWYANYDKPMLVTKVHRELTPLSYMEKNQQDKTYYEFTMSKYGNRIGDVVHKDKFMIEVRDLTEQLTFYVHNRGKFNAKSKAKMKVILIPELCFNFNFPGDLWLKLIFLPSILNRMYFLLHAEALRKRFNTYLNLHLLPFNGTDYMPRPLEIDYSLKRNVDPLGNVIPTEDIEEPKSLLEPMPTKSIEASVANLEITEFENPWQKYMEPVDLSRNLLSTYPVELDYYYHFSVGNVCEMNEMDFEDKEYWAKNQFHMPTGNIYGNRTPAKTNANVPALMPSKPTVRGKVKPLLILQKTVSKEHITPAEQGEFLAAITASSAADVFDMERLEILGDSFLKLSATLYLASKYSDWNEGTLTEVKSKLVSNRNLLFCLIDADIPKTLNTIQFTPRYTWLPPGISLPHNVLALWRENPEFAKIIGPHNLRDLALGDEESLVKGNCSDINYNRFVEGCRANGQSFYAGADFSSEVNFCVGLVTIPNKVIADTLEALLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQLLNLELGGKKMRANVNTTEIDGFLINHYYLEKNLGYTFKDRRYLLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLACICVRHRLHFFILAENAKLSEIISKFVNFQESQGHRVTNYVRILLEEADVQPTPLDLDDELDMTELPHANKCISQEAEKGVPPKGEFNMSTNVDVPKALGDVLEALIAAVYLDCRDLQRTWEVIFNLFEPELQEFTRKVPINHIRQLVEHKHAKPVFSSPIVEGETVMVSCQFTCMEKTIKVYGFGSNKDQAKLSAAKHALQQLSKCDA

Target ORF information:

RefSeq Version	NM_079054.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079054.5

ORF Insert Sequence:

ATGGAAGATG TGGAAATCAA GCCTCGCGGC TATCAACTTC GCCTGGTTGA CCACCTAACG 
AAGAGCAATG GCATTGTCTA CCTGCCCACA GGATCTGGGA AAACGTTCGT GGCCATCCTG 
GTCCTAAAGC GCTTCTCGCA GGACTTTGAC AAACCCATCG AAAGCGGCGG AAAGCGTGCG 
TTGTTCATGT GCAATACTGT GGAATTGGCC CGACAACAGG CGATGGCCGT CAGGCGGTGC 
ACCAACTTTA AGGTTGGCTT TTATGTGGGT GAACAGGGAG TGGATGACTG GACGCGCGGA 
ATGTGGAGTG ATGAAATCAA AAAAAACCAA GTTCTTGTTG GCACAGCCCA GGTCTTTTTG 
GACATGGTCA CCCAGACGTA CGTGGCTTTA AGCTCCCTGA GCGTTGTCAT CATAGACGAG 
TGCCACCACG GCACTGGTCA CCACCCATTT CGTGAGTTCA TGCGATTGTT CACTATCGCT 
AATCAAACAA AATTGCCGCG CGTGGTGGGT CTCACCGGGG TGTTAATCAA GGGAAATGAA 
ATTACGAACG TGGCCACGAA ACTTAAAGAG CTAGAGATCA CATATCGGGG AAACATCATT 
ACCGTGTCCG ACACCAAGGA GATGGAAAAC GTGATGCTGT ACGCCACCAA GCCCACAGAG 
GTCATGGTGT CCTTTCCACA TCAAGAGCAA GTGCTCACGG TTACAAGGTT AATAAGCGCA 
GAAATTGAAA AGTTCTATGT ATCCTTAGAC CTCATGAATA TTGGCGTTCA ACCGATCCGA 
AGGTCAAAGT CATTGCAATG CCTGCGGGAT CCGTCGAAGA AGAGCTTTGT AAAGCAACTG 
TTCAACGATT TTCTGTACCA GATGAAAGAG TATGGCATCT ACGCAGCTTC CATAGCCATA 
ATCTCGTTAA TTGTTGAGTT CGATATAAAG AGGCGCCAGG CCGAGACGCT AAGCGTGAAA 
TTGATGCATA GGACGGCCTT GACCTTGTGC GAGAAAATCC GTCACTTGCT GGTGCAAAAA 
CTGCAAGACA TGACTTATGA CGACGACGAT GACAACGTAA ACACTGAGGA AGTCATTATG 
AACTTCTCCA CCCCGAAAGT GCAGCGATTC CTGATGAGTC TTAAGGTATC CTTCGCTGAT 
AAAGATCCCA AAGATATCTG CTGTCTGGTC TTTGTTGAAC GCCGCTACAC CTGCAAGTGC 
ATCTATGGCT TGCTGCTCAA CTACATCCAG TCTACGCCTG AGCTGCGAAA CGTGCTCACG 
CCCCAATTCA TGGTGGGCCG CAACAATATT TCTCCAGACT TTGAGAGCGT CTTGGAGAGA 
AAATGGCAAA AATCTGCCAT TCAGCAGTTT CGGGACGGTA ATGCCAATCT TATGATATGT 
TCAAGCGTCC TAGAAGAAGG CATCGATGTG CAGGCCTGCA ATCATGTCTT TATTCTTGAT 
CCTGTTAAAA CCTTTAACAT GTACGTGCAG TCAAAGGGTC GGGCTCGTAC CACTGAGGCC 
AAATTCGTGC TGTTCACAGC TGATAAGGAA AGGGAAAAGA CAATTCAGCA AATTTATCAG 
TATCGAAAAG CGCATAATGA CATTGCCGAA TATTTAAAGG ATCGAGTTCT AGAAAAAACT 
GAGCCAGAAT TGTACGAAAT CAAAGGGCAC TTCCAGGACG ATATTGACCC TTTTACCAAT 
GAGAACGGTG CCGTGTTGCT TCCCAACAAT GCCCTGGCCA TACTGCATCG CTATTGCCAG 
ACAATTCCCA CGGATGCTTT TGGCTTTGTG ATCCCCTGGT TCCATGTATT GCAAGAAGAC 
GAACGTGATA GAATATTTGG TGTTTCTGCC AAGGGCAAGC ACGTAATTTC AATCAACATG 
CCAGTAAATT GCATGCTAAG AGACACAATT TACAGCGATC CCATGGACAA TGTTAAAACA 
GCCAAAATAT CAGCTGCCTT CAAAGCGTGT AAAGTACTCT ACAGTTTGGG AGAGTTAAAC 
GAGAGATTCG TGCCCAAAAC ATTAAAGGAG CGCGTAGCCT CTATTGCTGA TGTTCATTTT 
GAGCACTGGA ATAAGTATGG CGATAGTGTG ACTGCGACAG TCAACAAGGC CGATAAGAGC 
AAGGACAGAA CCTACAAGAC CGAATGTCCA TTAGAGTTTT ACGACGCACT TCCGCGGGTA 
GGTGAAATCT GTTATGCCTA CGAAATATTT TTGGAGCCGC AGTTTGAATC GTGTGAGTAC 
ACGGAACACA TGTATTTAAA TCTCCAAACA CCAAGAAATT ATGCTATATT GCTGCGGAAC 
AAGCTGCCAC GCCTTGCAGA GATGCCCCTG TTTAGCAATC AGGGTAAGCT ACACGTTCGA 
GTGGCCAATG CACCACTTGA AGTAATTATC CAGAACTCCG AACAGCTGGA GCTGCTCCAT 
CAGTTTCACG GAATGGTATT CCGCGATATC TTGAAGATTT GGCATCCGTT CTTTGTGCTG 
GATCGTCGCA GCAAGGAGAA CTCCTACCTG GTAGTTCCTC TCATACTGGG TGCGGGTGAA 
CAGAAATGCT TTGACTGGGA ACTGATGACC AACTTCCGAA GACTACCGCA AAGTCATGGG 
TCAAATGTAC AGCAACGCGA GCAACAACCT GCTCCACGTC CTGAAGATTT TGAGGGCAAA 
ATTGTTACAC AATGGTATGC CAACTATGAT AAGCCCATGC TGGTCACAAA GGTGCACCGT 
GAGCTAACAC CGTTAAGTTA TATGGAAAAG AACCAACAGG ACAAGACCTA CTACGAGTTT 
ACCATGTCAA AGTACGGCAA CCGCATTGGT GATGTTGTTC ACAAGGATAA GTTTATGATC 
GAGGTCAGAG ACCTTACGGA ACAGCTCACT TTCTACGTTC ATAATCGTGG AAAGTTCAAC 
GCGAAGAGCA AGGCTAAGAT GAAAGTTATT TTGATTCCGG AGCTATGCTT CAATTTTAAC 
TTTCCTGGGG ATTTATGGCT TAAGTTGATC TTCCTACCCA GCATTTTAAA CCGCATGTAC 
TTCCTTCTCC ACGCAGAGGC CTTACGTAAG CGATTTAATA CGTATTTAAA CCTCCATCTG 
CTGCCTTTTA ATGGAACTGA TTACATGCCC AGACCACTAG AAATTGATTA TTCGCTAAAG 
CGGAATGTCG ACCCCTTGGG CAATGTCATA CCAACTGAGG ATATCGAGGA GCCGAAATCC 
CTTTTAGAGC CAATGCCCAC AAAGTCCATT GAGGCGTCCG TGGCCAATCT TGAAATAACA 
GAATTCGAAA ATCCCTGGCA AAAGTATATG GAGCCGGTTG ATCTGTCGCG AAATCTTTTG 
AGTACGTATC CCGTAGAGCT GGACTACTAC TATCATTTTA GCGTTGGTAA TGTATGTGAG 
ATGAATGAGA TGGATTTTGA AGATAAGGAA TACTGGGCAA AAAATCAGTT CCATATGCCC 
ACGGGTAATA TCTATGGCAA CAGAACTCCG GCCAAGACTA ACGCAAACGT GCCAGCTTTG 
ATGCCGTCGA AACCGACTGT CCGCGGGAAG GTAAAACCAC TGCTCATTTT GCAAAAAACT 
GTTTCCAAAG AACATATAAC GCCAGCGGAG CAGGGCGAGT TTTTGGCTGC AATCACTGCC 
TCGAGCGCAG CGGATGTTTT CGACATGGAA CGCCTTGAGA TTCTGGGCGA TTCCTTCTTA 
AAACTTAGTG CCACGCTCTA CTTGGCCAGC AAGTATTCGG ATTGGAATGA GGGCACTCTT 
ACAGAGGTCA AATCCAAGCT TGTGTCTAAT CGTAACCTGT TGTTCTGCCT CATCGACGCG 
GACATTCCCA AAACGCTCAA CACCATTCAG TTTACGCCTA GATACACATG GCTTCCACCT 
GGCATCAGTT TGCCCCACAA TGTTCTAGCC TTGTGGCGAG AAAATCCGGA ATTCGCAAAG 
ATTATTGGCC CACACAATCT CCGTGACTTG GCTCTCGGCG ATGAGGAGTC CCTGGTGAAG 
GGCAACTGCA GCGATATCAA CTACAACCGC TTCGTGGAGG GATGTCGAGC AAATGGGCAG 
TCCTTTTATG CCGGTGCCGA TTTTTCATCC GAGGTGAACT TCTGCGTGGG ACTGGTCACG 
ATTCCCAACA AGGTGATAGC CGATACCCTT GAGGCGCTAT TGGGCGTAAT CGTTAAGAAC 
TACGGATTGC AGCATGCCTT CAAGATGCTA GAGTACTTCA AGATTTGCCG TGCGGATATT 
GACAAGCCGC TTACTCAGTT ACTGAACCTC GAACTGGGCG GCAAGAAAAT GCGGGCGAAC 
GTGAATACTA CAGAAATTGA CGGCTTTCTC ATAAATCACT ATTATTTGGA AAAAAATCTT 
GGCTACACGT TTAAGGATCG ACGATATTTG TTGCAGGCCC TCACCCATCC CTCGTATCCC 
ACTAATCGCA TCACTGGGTC CTATCAGGAG CTGGAATTTA TTGGCGATGC CATACTCGAC 
TTTCTAATCT CTGCCTATAT TTTCGAGAAC AATACGAAAA TGAATCCGGG CGCCCTCACA 
GATCTGCGTT CCGCTTTGGT CAACAATACT ACGCTGGCCT GCATTTGCGT GCGGCATCGG 
TTGCACTTTT TTATCCTAGC GGAGAACGCA AAGCTCTCCG AGATCATATC CAAATTCGTG 
AATTTCCAGG AGAGCCAGGG TCACCGGGTT ACCAACTATG TGCGCATTCT GCTCGAGGAA 
GCTGATGTTC AGCCGACTCC GCTGGACTTG GATGACGAAT TGGATATGAC TGAGTTACCG 
CATGCAAATA AATGCATTTC TCAGGAAGCA GAGAAAGGAG TCCCACCAAA AGGTGAGTTC 
AACATGTCCA CAAACGTGGA TGTGCCAAAG GCGTTGGGGG ATGTTCTGGA GGCCCTTATT 
GCAGCCGTAT ACCTGGACTG CCGAGATCTC CAACGTACTT GGGAGGTAAT TTTCAATCTC 
TTCGAGCCGG AGCTTCAGGA GTTCACGCGC AAAGTGCCCA TCAATCATAT ACGACAGCTC 
GTTGAGCACA AGCATGCGAA GCCCGTGTTT AGCTCGCCCA TCGTCGAAGG GGAAACTGTA 
ATGGTTAGTT GTCAATTTAC CTGCATGGAA AAGACGATCA AGGTGTACGG ATTTGGGAGC 
AATAAGGACC AGGCCAAGCT ATCGGCTGCC AAGCACGCCC TTCAGCAGCT AAGCAAATGC 
GACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcr-2 ( NM_001299611.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286540.1 Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21845
Clone ID Related Accession (Same CDS sequence)	NM_001299611.1
Accession Version	NM_001299611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dicer-2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGATG TGGAAATCAA GCCTCGCGGC TATCAACTTC GCCTGGTTGA CCACCTAACG 
AAGAGCAATG GCATTGTCTA CCTGCCCACA GGATCTGGGA AAACGTTCGT GGCCATCCTG 
GTCCTAAAGC GCTTCTCGCA GGACTTTGAC AAACCCATCG AAAGCGGCGG AAAGCGTGCG 
TTGTTCATGT GCAATACTGT GGAATTGGCC CGACAACAGG CGATGGCCGT CAGGCGGTGC 
ACCAACTTTA AGGTTGGCTT TTATGTGGGT GAACAGGGAG TGGATGACTG GACGCGCGGA 
ATGTGGAGTG ATGAAATCAA AAAAAACCAA GTTCTTGTTG GCACAGCCCA GGTCTTTTTG 
GACATGGTCA CCCAGACGTA CGTGGCTTTA AGCTCCCTGA GCGTTGTCAT CATAGACGAG 
TGCCACCACG GCACTGGTCA CCACCCATTT CGTGAGTTCA TGCGATTGTT CACTATCGCT 
AATCAAACAA AATTGCCGCG CGTGGTGGGT CTCACCGGGG TGTTAATCAA GGGAAATGAA 
ATTACGAACG TGGCCACGAA ACTTAAAGAG CTAGAGATCA CATATCGGGG AAACATCATT 
ACCGTGTCCG ACACCAAGGA GATGGAAAAC GTGATGCTGT ACGCCACCAA GCCCACAGAG 
GTCATGGTGT CCTTTCCACA TCAAGAGCAA GTGCTCACGG TTACAAGGTT AATAAGCGCA 
GAAATTGAAA AGTTCTATGT ATCCTTAGAC CTCATGAATA TTGGCGTTCA ACCGATCCGA 
AGGTCAAAGT CATTGCAATG CCTGCGGGAT CCGTCGAAGA AGAGCTTTGT AAAGCAACTG 
TTCAACGATT TTCTGTACCA GATGAAAGAG TATGGCATCT ACGCAGCTTC CATAGCCATA 
ATCTCGTTAA TTGTTGAGTT CGATATAAAG AGGCGCCAGG CCGAGACGCT AAGCGTGAAA 
TTGATGCATA GGACGGCCTT GACCTTGTGC GAGAAAATCC GTCACTTGCT GGTGCAAAAA 
CTGCAAGACA TGACTTATGA CGACGACGAT GACAACGTAA ACACTGAGGA AGTCATTATG 
AACTTCTCCA CCCCGAAAGT GCAGCGATTC CTGATGAGTC TTAAGGTATC CTTCGCTGAT 
AAAGATCCCA AAGATATCTG CTGTCTGGTC TTTGTTGAAC GCCGCTACAC CTGCAAGTGC 
ATCTATGGCT TGCTGCTCAA CTACATCCAG TCTACGCCTG AGCTGCGAAA CGTGCTCACG 
CCCCAATTCA TGGTGGGCCG CAACAATATT TCTCCAGACT TTGAGAGCGT CTTGGAGAGA 
AAATGGCAAA AATCTGCCAT TCAGCAGTTT CGGGACGGTA ATGCCAATCT TATGATATGT 
TCAAGCGTCC TAGAAGAAGG CATCGATGTG CAGGCCTGCA ATCATGTCTT TATTCTTGAT 
CCTGTTAAAA CCTTTAACAT GTACGTGCAG TCAAAGGGTC GGGCTCGTAC CACTGAGGCC 
AAATTCGTGC TGTTCACAGC TGATAAGGAA AGGGAAAAGA CAATTCAGCA AATTTATCAG 
TATCGAAAAG CGCATAATGA CATTGCCGAA TATTTAAAGG ATCGAGTTCT AGAAAAAACT 
GAGCCAGAAT TGTACGAAAT CAAAGGGCAC TTCCAGGACG ATATTGACCC TTTTACCAAT 
GAGAACGGTG CCGTGTTGCT TCCCAACAAT GCCCTGGCCA TACTGCATCG CTATTGCCAG 
ACAATTCCCA CGGATGCTTT TGGCTTTGTG ATCCCCTGGT TCCATGTATT GCAAGAAGAC 
GAACGTGATA GAATATTTGG TGTTTCTGCC AAGGGCAAGC ACGTAATTTC AATCAACATG 
CCAGTAAATT GCATGCTAAG AGACACAATT TACAGCGATC CCATGGACAA TGTTAAAACA 
GCCAAAATAT CAGCTGCCTT CAAAGCGTGT AAAGTACTCT ACAGTTTGGG AGAGTTAAAC 
GAGAGATTCG TGCCCAAAAC ATTAAAGGAG CGCGTAGCCT CTATTGCTGA TGTTCATTTT 
GAGCACTGGA ATAAGTATGG CGATATGACT GCGACAGTCA ACAAGGCCGA TAAGAGCAAG 
GACAGAACCT ACAAGACCGA ATGTCCATTA GAGTTTTACG ACGCACTTCC GCGGGTAGGT 
GAAATCTGTT ATGCCTACGA AATATTTTTG GAGCCGCAGT TTGAATCGTG TGAGTACACG 
GAACACATGT ATTTAAATCT CCAAACACCA AGAAATTATG CTATATTGCT GCGGAACAAG 
CTGCCACGCC TTGCAGAGAT GCCCCTGTTT AGCAATCAGG GTAAGCTACA CGTTCGAGTG 
GCCAATGCAC CACTTGAAGT AATTATCCAG AACTCCGAAC AGCTGGAGCT GCTCCATCAG 
TTTCACGGAA TGGTATTCCG CGATATCTTG AAGATTTGGC ATCCGTTCTT TGTGCTGGAT 
CGTCGCAGCA AGGAGAACTC CTACCTGGTA GTTCCTCTCA TACTGGGTGC GGGTGAACAG 
AAATGCTTTG ACTGGGAACT GATGACCAAC TTCCGAAGAC TACCGCAAAG TCATGGGTCA 
AATGTACAGC AACGCGAGCA ACAACCTGCT CCACGTCCTG AAGATTTTGA GGGCAAAATT 
GTTACACAAT GGTATGCCAA CTATGATAAG CCCATGCTGG TCACAAAGGT GCACCGTGAG 
CTAACACCGT TAAGTTATAT GGAAAAGAAC CAACAGGACA AGACCTACTA CGAGTTTACC 
ATGTCAAAGT ACGGCAACCG CATTGGTGAT GTTGTTCACA AGGATAAGTT TATGATCGAG 
GTCAGAGACC TTACGGAACA GCTCACTTTC TACGTTCATA ATCGTGGAAA GTTCAACGCG 
AAGAGCAAGG CTAAGATGAA AGTTATTTTG ATTCCGGAGC TATGCTTCAA TTTTAACTTT 
CCTGGGGATT TATGGCTTAA GTTGATCTTC CTACCCAGCA TTTTAAACCG CATGTACTTC 
CTTCTCCACG CAGAGGCCTT ACGTAAGCGA TTTAATACGT ATTTAAACCT CCATCTGCTG 
CCTTTTAATG GAACTGATTA CATGCCCAGA CCACTAGAAA TTGATTATTC GCTAAAGCGG 
AATGTCGACC CCTTGGGCAA TGTCATACCA ACTGAGGATA TCGAGGAGCC GAAATCCCTT 
TTAGAGCCAA TGCCCACAAA GTCCATTGAG GCGTCCGTGG CCAATCTTGA AATAACAGAA 
TTCGAAAATC CCTGGCAAAA GTATATGGAG CCGGTTGATC TGTCGCGAAA TCTTTTGAGT 
ACGTATCCCG TAGAGCTGGA CTACTACTAT CATTTTAGCG TTGGTAATGT ATGTGAGATG 
AATGAGATGG ATTTTGAAGA TAAGGAATAC TGGGCAAAAA ATCAGTTCCA TATGCCCACG 
GGTAATATCT ATGGCAACAG AACTCCGGCC AAGACTAACG CAAACGTGCC AGCTTTGATG 
CCGTCGAAAC CGACTGTCCG CGGGAAGGTA AAACCACTGC TCATTTTGCA AAAAACTGTT 
TCCAAAGAAC ATATAACGCC AGCGGAGCAG GGCGAGTTTT TGGCTGCAAT CACTGCCTCG 
AGCGCAGCGG ATGTTTTCGA CATGGAACGC CTTGAGATTC TGGGCGATTC CTTCTTAAAA 
CTTAGTGCCA CGCTCTACTT GGCCAGCAAG TATTCGGATT GGAATGAGGG CACTCTTACA 
GAGGTCAAAT CCAAGCTTGT GTCTAATCGT AACCTGTTGT TCTGCCTCAT CGACGCGGAC 
ATTCCCAAAA CGCTCAACAC CATTCAGTTT ACGCCTAGAT ACACATGGCT TCCACCTGGC 
ATCAGTTTGC CCCACAATGT TCTAGCCTTG TGGCGAGAAA ATCCGGAATT CGCAAAGATT 
ATTGGCCCAC ACAATCTCCG TGACTTGGCT CTCGGCGATG AGGAGTCCCT GGTGAAGGGC 
AACTGCAGCG ATATCAACTA CAACCGCTTC GTGGAGGGAT GTCGAGCAAA TGGGCAGTCC 
TTTTATGCCG GTGCCGATTT TTCATCCGAG GTGAACTTCT GCGTGGGACT GGTCACGATT 
CCCAACAAGG TGATAGCCGA TACCCTTGAG GCGCTATTGG GCGTAATCGT TAAGAACTAC 
GGATTGCAGC ATGCCTTCAA GATGCTAGAG TACTTCAAGA TTTGCCGTGC GGATATTGAC 
AAGCCGCTTA CTCAGTTACT GAACCTCGAA CTGGGCGGCA AGAAAATGCG GGCGAACGTG 
AATACTACAG AAATTGACGG CTTTCTCATA AATCACTATT ATTTGGAAAA AAATCTTGGC 
TACACGTTTA AGGATCGACG ATATTTGTTG CAGGCCCTCA CCCATCCCTC GTATCCCACT 
AATCGCATCA CTGGGTCCTA TCAGGAGCTG GAATTTATTG GCGATGCCAT ACTCGACTTT 
CTAATCTCTG CCTATATTTT CGAGAACAAT ACGAAAATGA ATCCGGGCGC CCTCACAGAT 
CTGCGTTCCG CTTTGGTCAA CAATACTACG CTGGCCTGCA TTTGCGTGCG GCATCGGTTG 
CACTTTTTTA TCCTAGCGGA GAACGCAAAG CTCTCCGAGA TCATATCCAA ATTCGTGAAT 
TTCCAGGAGA GCCAGGGTCA CCGGGTTACC AACTATGTGC GCATTCTGCT CGAGGAAGCT 
GATGTTCAGC CGACTCCGCT GGACTTGGAT GACGAATTGG ATATGACTGA GTTACCGCAT 
GCAAATAAAT GCATTTCTCA GGAAGCAGAG AAAGGAGTCC CACCAAAAGG TGAGTTCAAC 
ATGTCCACAA ACGTGGATGT GCCAAAGGCG TTGGGGGATG TTCTGGAGGC CCTTATTGCA 
GCCGTATACC TGGACTGCCG AGATCTCCAA CGTACTTGGG AGGTAATTTT CAATCTCTTC 
GAGCCGGAGC TTCAGGAGTT CACGCGCAAA GTGCCCATCA ATCATATACG ACAGCTCGTT 
GAGCACAAGC ATGCGAAGCC CGTGTTTAGC TCGCCCATCG TCGAAGGGGA AACTGTAATG 
GTTAGTTGTC AATTTACCTG CATGGAAAAG ACGATCAAGG TGTACGGATT TGGGAGCAAT 
AAGGACCAGG CCAAGCTATC GGCTGCCAAG CACGCCCTTC AGCAGCTAAG CAAATGCGAC 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286540.1
CDS	100..5265
Translation	MEDVEIKPRGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRFSQDFDKPIESGGKRALFMCNTVELARQQAMAVRRCTNFKVGFYVGEQGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVIIDECHHGTGHHPFREFMRLFTIANQTKLPRVVGLTGVLIKGNEITNVATKLKELEITYRGNIITVSDTKEMENVMLYATKPTEVMVSFPHQEQVLTVTRLISAEIEKFYVSLDLMNIGVQPIRRSKSLQCLRDPSKKSFVKQLFNDFLYQMKEYGIYAASIAIISLIVEFDIKRRQAETLSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEEVIMNFSTPKVQRFLMSLKVSFADKDPKDICCLVFVERRYTCKCIYGLLLNYIQSTPELRNVLTPQFMVGRNNISPDFESVLERKWQKSAIQQFRDGNANLMICSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFVLFTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLEKTEPELYEIKGHFQDDIDPFTNENGAVLLPNNALAILHRYCQTIPTDAFGFVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCMLRDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVPKTLKERVASIADVHFEHWNKYGDMTATVNKADKSKDRTYKTECPLEFYDALPRVGEICYAYEIFLEPQFESCEYTEHMYLNLQTPRNYAILLRNKLPRLAEMPLFSNQGKLHVRVANAPLEVIIQNSEQLELLHQFHGMVFRDILKIWHPFFVLDRRSKENSYLVVPLILGAGEQKCFDWELMTNFRRLPQSHGSNVQQREQQPAPRPEDFEGKIVTQWYANYDKPMLVTKVHRELTPLSYMEKNQQDKTYYEFTMSKYGNRIGDVVHKDKFMIEVRDLTEQLTFYVHNRGKFNAKSKAKMKVILIPELCFNFNFPGDLWLKLIFLPSILNRMYFLLHAEALRKRFNTYLNLHLLPFNGTDYMPRPLEIDYSLKRNVDPLGNVIPTEDIEEPKSLLEPMPTKSIEASVANLEITEFENPWQKYMEPVDLSRNLLSTYPVELDYYYHFSVGNVCEMNEMDFEDKEYWAKNQFHMPTGNIYGNRTPAKTNANVPALMPSKPTVRGKVKPLLILQKTVSKEHITPAEQGEFLAAITASSAADVFDMERLEILGDSFLKLSATLYLASKYSDWNEGTLTEVKSKLVSNRNLLFCLIDADIPKTLNTIQFTPRYTWLPPGISLPHNVLALWRENPEFAKIIGPHNLRDLALGDEESLVKGNCSDINYNRFVEGCRANGQSFYAGADFSSEVNFCVGLVTIPNKVIADTLEALLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQLLNLELGGKKMRANVNTTEIDGFLINHYYLEKNLGYTFKDRRYLLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLACICVRHRLHFFILAENAKLSEIISKFVNFQESQGHRVTNYVRILLEEADVQPTPLDLDDELDMTELPHANKCISQEAEKGVPPKGEFNMSTNVDVPKALGDVLEALIAAVYLDCRDLQRTWEVIFNLFEPELQEFTRKVPINHIRQLVEHKHAKPVFSSPIVEGETVMVSCQFTCMEKTIKVYGFGSNKDQAKLSAAKHALQQLSKCDA

Target ORF information:

RefSeq Version	NM_001299611.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dicer-2 (Dcr-2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299611.1

ORF Insert Sequence:

ATGGAAGATG TGGAAATCAA GCCTCGCGGC TATCAACTTC GCCTGGTTGA CCACCTAACG 
AAGAGCAATG GCATTGTCTA CCTGCCCACA GGATCTGGGA AAACGTTCGT GGCCATCCTG 
GTCCTAAAGC GCTTCTCGCA GGACTTTGAC AAACCCATCG AAAGCGGCGG AAAGCGTGCG 
TTGTTCATGT GCAATACTGT GGAATTGGCC CGACAACAGG CGATGGCCGT CAGGCGGTGC 
ACCAACTTTA AGGTTGGCTT TTATGTGGGT GAACAGGGAG TGGATGACTG GACGCGCGGA 
ATGTGGAGTG ATGAAATCAA AAAAAACCAA GTTCTTGTTG GCACAGCCCA GGTCTTTTTG 
GACATGGTCA CCCAGACGTA CGTGGCTTTA AGCTCCCTGA GCGTTGTCAT CATAGACGAG 
TGCCACCACG GCACTGGTCA CCACCCATTT CGTGAGTTCA TGCGATTGTT CACTATCGCT 
AATCAAACAA AATTGCCGCG CGTGGTGGGT CTCACCGGGG TGTTAATCAA GGGAAATGAA 
ATTACGAACG TGGCCACGAA ACTTAAAGAG CTAGAGATCA CATATCGGGG AAACATCATT 
ACCGTGTCCG ACACCAAGGA GATGGAAAAC GTGATGCTGT ACGCCACCAA GCCCACAGAG 
GTCATGGTGT CCTTTCCACA TCAAGAGCAA GTGCTCACGG TTACAAGGTT AATAAGCGCA 
GAAATTGAAA AGTTCTATGT ATCCTTAGAC CTCATGAATA TTGGCGTTCA ACCGATCCGA 
AGGTCAAAGT CATTGCAATG CCTGCGGGAT CCGTCGAAGA AGAGCTTTGT AAAGCAACTG 
TTCAACGATT TTCTGTACCA GATGAAAGAG TATGGCATCT ACGCAGCTTC CATAGCCATA 
ATCTCGTTAA TTGTTGAGTT CGATATAAAG AGGCGCCAGG CCGAGACGCT AAGCGTGAAA 
TTGATGCATA GGACGGCCTT GACCTTGTGC GAGAAAATCC GTCACTTGCT GGTGCAAAAA 
CTGCAAGACA TGACTTATGA CGACGACGAT GACAACGTAA ACACTGAGGA AGTCATTATG 
AACTTCTCCA CCCCGAAAGT GCAGCGATTC CTGATGAGTC TTAAGGTATC CTTCGCTGAT 
AAAGATCCCA AAGATATCTG CTGTCTGGTC TTTGTTGAAC GCCGCTACAC CTGCAAGTGC 
ATCTATGGCT TGCTGCTCAA CTACATCCAG TCTACGCCTG AGCTGCGAAA CGTGCTCACG 
CCCCAATTCA TGGTGGGCCG CAACAATATT TCTCCAGACT TTGAGAGCGT CTTGGAGAGA 
AAATGGCAAA AATCTGCCAT TCAGCAGTTT CGGGACGGTA ATGCCAATCT TATGATATGT 
TCAAGCGTCC TAGAAGAAGG CATCGATGTG CAGGCCTGCA ATCATGTCTT TATTCTTGAT 
CCTGTTAAAA CCTTTAACAT GTACGTGCAG TCAAAGGGTC GGGCTCGTAC CACTGAGGCC 
AAATTCGTGC TGTTCACAGC TGATAAGGAA AGGGAAAAGA CAATTCAGCA AATTTATCAG 
TATCGAAAAG CGCATAATGA CATTGCCGAA TATTTAAAGG ATCGAGTTCT AGAAAAAACT 
GAGCCAGAAT TGTACGAAAT CAAAGGGCAC TTCCAGGACG ATATTGACCC TTTTACCAAT 
GAGAACGGTG CCGTGTTGCT TCCCAACAAT GCCCTGGCCA TACTGCATCG CTATTGCCAG 
ACAATTCCCA CGGATGCTTT TGGCTTTGTG ATCCCCTGGT TCCATGTATT GCAAGAAGAC 
GAACGTGATA GAATATTTGG TGTTTCTGCC AAGGGCAAGC ACGTAATTTC AATCAACATG 
CCAGTAAATT GCATGCTAAG AGACACAATT TACAGCGATC CCATGGACAA TGTTAAAACA 
GCCAAAATAT CAGCTGCCTT CAAAGCGTGT AAAGTACTCT ACAGTTTGGG AGAGTTAAAC 
GAGAGATTCG TGCCCAAAAC ATTAAAGGAG CGCGTAGCCT CTATTGCTGA TGTTCATTTT 
GAGCACTGGA ATAAGTATGG CGATATGACT GCGACAGTCA ACAAGGCCGA TAAGAGCAAG 
GACAGAACCT ACAAGACCGA ATGTCCATTA GAGTTTTACG ACGCACTTCC GCGGGTAGGT 
GAAATCTGTT ATGCCTACGA AATATTTTTG GAGCCGCAGT TTGAATCGTG TGAGTACACG 
GAACACATGT ATTTAAATCT CCAAACACCA AGAAATTATG CTATATTGCT GCGGAACAAG 
CTGCCACGCC TTGCAGAGAT GCCCCTGTTT AGCAATCAGG GTAAGCTACA CGTTCGAGTG 
GCCAATGCAC CACTTGAAGT AATTATCCAG AACTCCGAAC AGCTGGAGCT GCTCCATCAG 
TTTCACGGAA TGGTATTCCG CGATATCTTG AAGATTTGGC ATCCGTTCTT TGTGCTGGAT 
CGTCGCAGCA AGGAGAACTC CTACCTGGTA GTTCCTCTCA TACTGGGTGC GGGTGAACAG 
AAATGCTTTG ACTGGGAACT GATGACCAAC TTCCGAAGAC TACCGCAAAG TCATGGGTCA 
AATGTACAGC AACGCGAGCA ACAACCTGCT CCACGTCCTG AAGATTTTGA GGGCAAAATT 
GTTACACAAT GGTATGCCAA CTATGATAAG CCCATGCTGG TCACAAAGGT GCACCGTGAG 
CTAACACCGT TAAGTTATAT GGAAAAGAAC CAACAGGACA AGACCTACTA CGAGTTTACC 
ATGTCAAAGT ACGGCAACCG CATTGGTGAT GTTGTTCACA AGGATAAGTT TATGATCGAG 
GTCAGAGACC TTACGGAACA GCTCACTTTC TACGTTCATA ATCGTGGAAA GTTCAACGCG 
AAGAGCAAGG CTAAGATGAA AGTTATTTTG ATTCCGGAGC TATGCTTCAA TTTTAACTTT 
CCTGGGGATT TATGGCTTAA GTTGATCTTC CTACCCAGCA TTTTAAACCG CATGTACTTC 
CTTCTCCACG CAGAGGCCTT ACGTAAGCGA TTTAATACGT ATTTAAACCT CCATCTGCTG 
CCTTTTAATG GAACTGATTA CATGCCCAGA CCACTAGAAA TTGATTATTC GCTAAAGCGG 
AATGTCGACC CCTTGGGCAA TGTCATACCA ACTGAGGATA TCGAGGAGCC GAAATCCCTT 
TTAGAGCCAA TGCCCACAAA GTCCATTGAG GCGTCCGTGG CCAATCTTGA AATAACAGAA 
TTCGAAAATC CCTGGCAAAA GTATATGGAG CCGGTTGATC TGTCGCGAAA TCTTTTGAGT 
ACGTATCCCG TAGAGCTGGA CTACTACTAT CATTTTAGCG TTGGTAATGT ATGTGAGATG 
AATGAGATGG ATTTTGAAGA TAAGGAATAC TGGGCAAAAA ATCAGTTCCA TATGCCCACG 
GGTAATATCT ATGGCAACAG AACTCCGGCC AAGACTAACG CAAACGTGCC AGCTTTGATG 
CCGTCGAAAC CGACTGTCCG CGGGAAGGTA AAACCACTGC TCATTTTGCA AAAAACTGTT 
TCCAAAGAAC ATATAACGCC AGCGGAGCAG GGCGAGTTTT TGGCTGCAAT CACTGCCTCG 
AGCGCAGCGG ATGTTTTCGA CATGGAACGC CTTGAGATTC TGGGCGATTC CTTCTTAAAA 
CTTAGTGCCA CGCTCTACTT GGCCAGCAAG TATTCGGATT GGAATGAGGG CACTCTTACA 
GAGGTCAAAT CCAAGCTTGT GTCTAATCGT AACCTGTTGT TCTGCCTCAT CGACGCGGAC 
ATTCCCAAAA CGCTCAACAC CATTCAGTTT ACGCCTAGAT ACACATGGCT TCCACCTGGC 
ATCAGTTTGC CCCACAATGT TCTAGCCTTG TGGCGAGAAA ATCCGGAATT CGCAAAGATT 
ATTGGCCCAC ACAATCTCCG TGACTTGGCT CTCGGCGATG AGGAGTCCCT GGTGAAGGGC 
AACTGCAGCG ATATCAACTA CAACCGCTTC GTGGAGGGAT GTCGAGCAAA TGGGCAGTCC 
TTTTATGCCG GTGCCGATTT TTCATCCGAG GTGAACTTCT GCGTGGGACT GGTCACGATT 
CCCAACAAGG TGATAGCCGA TACCCTTGAG GCGCTATTGG GCGTAATCGT TAAGAACTAC 
GGATTGCAGC ATGCCTTCAA GATGCTAGAG TACTTCAAGA TTTGCCGTGC GGATATTGAC 
AAGCCGCTTA CTCAGTTACT GAACCTCGAA CTGGGCGGCA AGAAAATGCG GGCGAACGTG 
AATACTACAG AAATTGACGG CTTTCTCATA AATCACTATT ATTTGGAAAA AAATCTTGGC 
TACACGTTTA AGGATCGACG ATATTTGTTG CAGGCCCTCA CCCATCCCTC GTATCCCACT 
AATCGCATCA CTGGGTCCTA TCAGGAGCTG GAATTTATTG GCGATGCCAT ACTCGACTTT 
CTAATCTCTG CCTATATTTT CGAGAACAAT ACGAAAATGA ATCCGGGCGC CCTCACAGAT 
CTGCGTTCCG CTTTGGTCAA CAATACTACG CTGGCCTGCA TTTGCGTGCG GCATCGGTTG 
CACTTTTTTA TCCTAGCGGA GAACGCAAAG CTCTCCGAGA TCATATCCAA ATTCGTGAAT 
TTCCAGGAGA GCCAGGGTCA CCGGGTTACC AACTATGTGC GCATTCTGCT CGAGGAAGCT 
GATGTTCAGC CGACTCCGCT GGACTTGGAT GACGAATTGG ATATGACTGA GTTACCGCAT 
GCAAATAAAT GCATTTCTCA GGAAGCAGAG AAAGGAGTCC CACCAAAAGG TGAGTTCAAC 
ATGTCCACAA ACGTGGATGT GCCAAAGGCG TTGGGGGATG TTCTGGAGGC CCTTATTGCA 
GCCGTATACC TGGACTGCCG AGATCTCCAA CGTACTTGGG AGGTAATTTT CAATCTCTTC 
GAGCCGGAGC TTCAGGAGTT CACGCGCAAA GTGCCCATCA ATCATATACG ACAGCTCGTT 
GAGCACAAGC ATGCGAAGCC CGTGTTTAGC TCGCCCATCG TCGAAGGGGA AACTGTAATG 
GTTAGTTGTC AATTTACCTG CATGGAAAAG ACGATCAAGG TGTACGGATT TGGGAGCAAT 
AAGGACCAGG CCAAGCTATC GGCTGCCAAG CACGCCCTTC AGCAGCTAAG CAAATGCGAC 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dcr-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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