CG8405 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8405
Entrez Gene ID	36772
Full Name	CG8405 gene product from transcript CG8405-RB
Synonyms	Dmel\CG8405,R5_2R:11947059..11948303
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E1-52E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_166137.2	NP_725521.1	uncharacterized protein, isoform A
NM_001299546.1	NP_001286475.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y59C2A.2	NP_494212.3
Drosophila melanogaster (fruit fly)	CG8405	NP_725521.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

genomic organization, expression, and evolutionary conservation
Title: Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.

The following CG8405 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8405 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10157	NM_001299546.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8405), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa10157	NM_166137.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8405), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8405 ( NM_166137.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725521.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8405), mRNA.

Price & Availability↑

CloneID	OFa10157
Clone ID Related Accession (Same CDS sequence)	NM_166137.2 , NM_001299546.1
Accession Version	NM_166137.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166137.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCTG TGCTGCCGGA AAACGCGGCC GAGATCGCGG TGGCCAATGC AATCAATAAC 
ATCAACGGGA ATGGAAATGG GAACGGGCGC AATCGGGTGC TACCTGGCGG ATTAGGTGGT 
GGTGGCCAGA ACAACATGAT GAATATGCGG GATCGACTGT TCCATGCCAT ATACTTTCGG 
GCGGCGGCCA CCTATGCGGA GTTGGTTCCA AGGAAGGTGC AGTTGACCAT TGAGTTCCTG 
CTGCTGGCCA AGGCCTTGCT GTTCTTCTTT ACGCTCATCT ACGTGCACAA CTCCTTCATC 
AAGAATCCCT GCACTTGCCT GCAGGATGTT AAGAACTGGC CGCGCGAAGG CGTCATCAGA 
GTGGAAATTA TTCCTCACCT GGCGGAGAAG CGTGCAATTT GGCAGGGAAT TAAAAGGGAC 
CAAGAAATAG TGCGCTCCCT CAAGGATTCC TACTACTATG GTATTGGACC CCAAACTCGT 
CAAAATCACG AAACGCTTAA ACGGTACGAG TCCATTCTGG GAAAACTGGG TCTGCCCGTC 
CGACCAACAT TCGCTTACAG TAACGAATCC CTGTACTACT ACTTTGATGC CATCAACATC 
CTGGACACCT ACGACCATCC AAATGCAATA CAGCTCAAGA ACGAAGAGGA TGATGAAGAG 
CAGTACATTG TGGAGTATTC ATTGGAATAC GGCCACCTCC GCTTGTCTTC ATCCACGCGA 
AAGCGTCTAC GCATACCCGT ACTCACTGTC CAACTGGATC CCAATACGGA CAAGTGTTTT 
GGGGACAGTT TGACGCGATA TCTGCTCAAA CGTTTGCTAG GCTATGATGA CTTACTAATG 
GCATCCGTAC GAACAATAGC CGAGCAGGAG GAAAATAAGG GCTACCTGCG CAATGTTATA 
ACCGGCGAAC ATTATCGCTT CGTCTCCATG TGGTGGGCAG CCTGGAGCAG TTACCCGGCT 
GCCTTCTGTG TTATGTTACT ATTCACGTTC TCAGTGTCGA TGTTGCTGCG CTACTCACAC 
CACCAGATCT TTGTGTTTAT AGTCGACCTG CTGCAGATGC TGGAGTACAA TGTGTCTGCC 
CGGTTCCCAA TCGCACCGTT GCTCACTGTC ATATTGGCGC TCGTAGGAAT GGAAGCCATC 
ATGTCGGAGT TCTTTAATGA CACCACTACG GCCTTCTACA TCATACTGAT TGTCTGGATC 
GCGGATCAAT ACGATGCCAT CTGCTGTCAC ACGAGCATCA CCAAGCGTCA TTGGCTCAGA 
TTCTTCTACC TGTACCATTT TGCGTTCTAT GCGTATCACT ATCGCTTCAG TGGACAGTAC 
AGGACGCTGG CCCTGCTCTC GTCGTACCTA TTCACCCAGC ACTCGATGGT GTTCTTCTTT 
CATCGCTACG AGCTGCCAGC CATCATGGCC CAGCACCAGG TCTTCATCAT TACACGCAAC 
CAGGGAGCAG CAGCAGCAGC TGCTGCAGCG GCAGCGGGCG CGGGCGGAGC AGAGGCAGCA 
GGAGGGGGCG CAGGAGCAGG CGCTGCAGCT GGTGGACAGC AAGGAGATGG TGGGGCAGGA 
GGAGGCGGCA TCGGTGCTGG CCAGCAGGCG TCCATGCAAC GTGCCCGTCT CATAGTCACT 
CGGCTCTTCA GTCAACTTGC AGCACAACGC CATCAGCAGG AACCACGCCC GCCAGGACCC 
ACTGCCGCCG TGGGCGTGGC CTTGGCCAAC CTGAGAAGAC TGCCCGTGGT GGGCCAGTTG 
TTGCAGGGCC GCGAGCAATT CGCCACCGTG CGTCGCGTCT TCCTGGGACA TGGCGGCAAC 
GCAAGGCTGA TGCAGGTGCG TCTGCAGCGT ATTAACGTCG CTGACATACC TGGCTTGCGC 
AACCAGGTGG CAGCGGCCAT GGCAGCAGCG GGGGCCGCCC AAAGGGGCCA GAGCGCTGCA 
ACAGATGAAC CCACCATCGG CGATGTTGCA ACTACAGATG TGGCAGCAGC AGCAGACGCG 
GCAGCAACGC CAGCAGCTAA CGATGCAGCA GATGTCGAAA CAAATCAGGG ACGCAAGCAG 
CCGACAACAT CGAGCATAAA TGATGCAGTA GCAACGGGAG CGGAAGGAGC TCAGGACAAG 
CCCGGCCAGG AGAAAGTGGA GGTGGTGGAG AATTCTGGGC AAGAAAACCT ACAGCATGGC 
GATGGGGATG GGCAAATAGC GGGCATGTCC AAGACGCTGG AAAATCTAAA TTTCGTTGCG 
GCGGATATTA TAAATATGTC GGATGAAACT ACTGTAGCAT CATCGCCCCC AACGAACGGC 
ACTCAACAAC CAGATCAGAC CCTGATCCAC ACCCACGTCC ATGTCCATCA GACTCAGCCA 
AAGGCGAGGC AATTAGAAAA TAATTTGCCA GAGTTGCTGA AAGCTGAGGG GGGGGCATCC 
GCAGCAACGG CATCAGGTGA AAAGCAAAGC TGCCCACTAC AAGCACCCAG CATTATGAAT 
GACCAGCAGC AGCAGCAGCA GCAGCAGCAG AGGCAGAAAA ACAAACCGGA CGAGCCCGCG 
ACAGCAACGG TAGCAACATC AGCAGTTGCC ACGTCAAAAC AATCCTCTGG GCCAGGAGCA 
ACAGAGGTAG GAGTGGTAGG AGCGGCAGGA GGAGCCGAGG CAACAGCCAG GCCAATGATG 
GATGACAGGC AAACAGTTGA TTTTATAGAA GGCGACAGCC CAACGTCAGC AGCAAATTGC 
CAACTCAATG CTGGCGCAAA CCACAAGGCA CAGGAGCAGC AGCTAATGAA AACTTCACAA 
CATACATCAA ACCAAGTGAC AACAGCAGCA GCAACAGGAG CAGCAGCAGG AACACCAGCA 
ACAGTAACAC AAGCAACTGG AGCAACAGCC GCAATGGGAA CAGCAGAAAC ATCAGCAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725521.1
CDS	147..3029
Translation	MAAVLPENAAEIAVANAINNINGNGNGNGRNRVLPGGLGGGGQNNMMNMRDRLFHAIYFRAAATYAELVPRKVQLTIEFLLLAKALLFFFTLIYVHNSFIKNPCTCLQDVKNWPREGVIRVEIIPHLAEKRAIWQGIKRDQEIVRSLKDSYYYGIGPQTRQNHETLKRYESILGKLGLPVRPTFAYSNESLYYYFDAINILDTYDHPNAIQLKNEEDDEEQYIVEYSLEYGHLRLSSSTRKRLRIPVLTVQLDPNTDKCFGDSLTRYLLKRLLGYDDLLMASVRTIAEQEENKGYLRNVITGEHYRFVSMWWAAWSSYPAAFCVMLLFTFSVSMLLRYSHHQIFVFIVDLLQMLEYNVSARFPIAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWIADQYDAICCHTSITKRHWLRFFYLYHFAFYAYHYRFSGQYRTLALLSSYLFTQHSMVFFFHRYELPAIMAQHQVFIITRNQGAAAAAAAAAAGAGGAEAAGGGAGAGAAAGGQQGDGGAGGGGIGAGQQASMQRARLIVTRLFSQLAAQRHQQEPRPPGPTAAVGVALANLRRLPVVGQLLQGREQFATVRRVFLGHGGNARLMQVRLQRINVADIPGLRNQVAAAMAAAGAAQRGQSAATDEPTIGDVATTDVAAAADAAATPAANDAADVETNQGRKQPTTSSINDAVATGAEGAQDKPGQEKVEVVENSGQENLQHGDGDGQIAGMSKTLENLNFVAADIINMSDETTVASSPPTNGTQQPDQTLIHTHVHVHQTQPKARQLENNLPELLKAEGGASAATASGEKQSCPLQAPSIMNDQQQQQQQQQRQKNKPDEPATATVATSAVATSKQSSGPGATEVGVVGAAGGAEATARPMMDDRQTVDFIEGDSPTSAANCQLNAGANHKAQEQQLMKTSQHTSNQVTTAAATGAAAGTPATVTQATGATAAMGTAETSAE

Target ORF information:

RefSeq Version	NM_166137.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8405), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166137.2

ORF Insert Sequence:

ATGGCCGCTG TGCTGCCGGA AAACGCGGCC GAGATCGCGG TGGCCAATGC AATCAATAAC 
ATCAACGGGA ATGGAAATGG GAACGGGCGC AATCGGGTGC TACCTGGCGG ATTAGGTGGT 
GGTGGCCAGA ACAACATGAT GAATATGCGG GATCGACTGT TCCATGCCAT ATACTTTCGG 
GCGGCGGCCA CCTATGCGGA GTTGGTTCCA AGGAAGGTGC AGTTGACCAT TGAGTTCCTG 
CTGCTGGCCA AGGCCTTGCT GTTCTTCTTT ACGCTCATCT ACGTGCACAA CTCCTTCATC 
AAGAATCCCT GCACTTGCCT GCAGGATGTT AAGAACTGGC CGCGCGAAGG CGTCATCAGA 
GTGGAAATTA TTCCTCACCT GGCGGAGAAG CGTGCAATTT GGCAGGGAAT TAAAAGGGAC 
CAAGAAATAG TGCGCTCCCT CAAGGATTCC TACTACTATG GTATTGGACC CCAAACTCGT 
CAAAATCACG AAACGCTTAA ACGGTACGAG TCCATTCTGG GAAAACTGGG TCTGCCCGTC 
CGACCAACAT TCGCTTACAG TAACGAATCC CTGTACTACT ACTTTGATGC CATCAACATC 
CTGGACACCT ACGACCATCC AAATGCAATA CAGCTCAAGA ACGAAGAGGA TGATGAAGAG 
CAGTACATTG TGGAGTATTC ATTGGAATAC GGCCACCTCC GCTTGTCTTC ATCCACGCGA 
AAGCGTCTAC GCATACCCGT ACTCACTGTC CAACTGGATC CCAATACGGA CAAGTGTTTT 
GGGGACAGTT TGACGCGATA TCTGCTCAAA CGTTTGCTAG GCTATGATGA CTTACTAATG 
GCATCCGTAC GAACAATAGC CGAGCAGGAG GAAAATAAGG GCTACCTGCG CAATGTTATA 
ACCGGCGAAC ATTATCGCTT CGTCTCCATG TGGTGGGCAG CCTGGAGCAG TTACCCGGCT 
GCCTTCTGTG TTATGTTACT ATTCACGTTC TCAGTGTCGA TGTTGCTGCG CTACTCACAC 
CACCAGATCT TTGTGTTTAT AGTCGACCTG CTGCAGATGC TGGAGTACAA TGTGTCTGCC 
CGGTTCCCAA TCGCACCGTT GCTCACTGTC ATATTGGCGC TCGTAGGAAT GGAAGCCATC 
ATGTCGGAGT TCTTTAATGA CACCACTACG GCCTTCTACA TCATACTGAT TGTCTGGATC 
GCGGATCAAT ACGATGCCAT CTGCTGTCAC ACGAGCATCA CCAAGCGTCA TTGGCTCAGA 
TTCTTCTACC TGTACCATTT TGCGTTCTAT GCGTATCACT ATCGCTTCAG TGGACAGTAC 
AGGACGCTGG CCCTGCTCTC GTCGTACCTA TTCACCCAGC ACTCGATGGT GTTCTTCTTT 
CATCGCTACG AGCTGCCAGC CATCATGGCC CAGCACCAGG TCTTCATCAT TACACGCAAC 
CAGGGAGCAG CAGCAGCAGC TGCTGCAGCG GCAGCGGGCG CGGGCGGAGC AGAGGCAGCA 
GGAGGGGGCG CAGGAGCAGG CGCTGCAGCT GGTGGACAGC AAGGAGATGG TGGGGCAGGA 
GGAGGCGGCA TCGGTGCTGG CCAGCAGGCG TCCATGCAAC GTGCCCGTCT CATAGTCACT 
CGGCTCTTCA GTCAACTTGC AGCACAACGC CATCAGCAGG AACCACGCCC GCCAGGACCC 
ACTGCCGCCG TGGGCGTGGC CTTGGCCAAC CTGAGAAGAC TGCCCGTGGT GGGCCAGTTG 
TTGCAGGGCC GCGAGCAATT CGCCACCGTG CGTCGCGTCT TCCTGGGACA TGGCGGCAAC 
GCAAGGCTGA TGCAGGTGCG TCTGCAGCGT ATTAACGTCG CTGACATACC TGGCTTGCGC 
AACCAGGTGG CAGCGGCCAT GGCAGCAGCG GGGGCCGCCC AAAGGGGCCA GAGCGCTGCA 
ACAGATGAAC CCACCATCGG CGATGTTGCA ACTACAGATG TGGCAGCAGC AGCAGACGCG 
GCAGCAACGC CAGCAGCTAA CGATGCAGCA GATGTCGAAA CAAATCAGGG ACGCAAGCAG 
CCGACAACAT CGAGCATAAA TGATGCAGTA GCAACGGGAG CGGAAGGAGC TCAGGACAAG 
CCCGGCCAGG AGAAAGTGGA GGTGGTGGAG AATTCTGGGC AAGAAAACCT ACAGCATGGC 
GATGGGGATG GGCAAATAGC GGGCATGTCC AAGACGCTGG AAAATCTAAA TTTCGTTGCG 
GCGGATATTA TAAATATGTC GGATGAAACT ACTGTAGCAT CATCGCCCCC AACGAACGGC 
ACTCAACAAC CAGATCAGAC CCTGATCCAC ACCCACGTCC ATGTCCATCA GACTCAGCCA 
AAGGCGAGGC AATTAGAAAA TAATTTGCCA GAGTTGCTGA AAGCTGAGGG GGGGGCATCC 
GCAGCAACGG CATCAGGTGA AAAGCAAAGC TGCCCACTAC AAGCACCCAG CATTATGAAT 
GACCAGCAGC AGCAGCAGCA GCAGCAGCAG AGGCAGAAAA ACAAACCGGA CGAGCCCGCG 
ACAGCAACGG TAGCAACATC AGCAGTTGCC ACGTCAAAAC AATCCTCTGG GCCAGGAGCA 
ACAGAGGTAG GAGTGGTAGG AGCGGCAGGA GGAGCCGAGG CAACAGCCAG GCCAATGATG 
GATGACAGGC AAACAGTTGA TTTTATAGAA GGCGACAGCC CAACGTCAGC AGCAAATTGC 
CAACTCAATG CTGGCGCAAA CCACAAGGCA CAGGAGCAGC AGCTAATGAA AACTTCACAA 
CATACATCAA ACCAAGTGAC AACAGCAGCA GCAACAGGAG CAGCAGCAGG AACACCAGCA 
ACAGTAACAC AAGCAACTGG AGCAACAGCC GCAATGGGAA CAGCAGAAAC ATCAGCAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8405 ( NM_001299546.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286475.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8405), mRNA.

Price & Availability↑

CloneID	OFa10157
Clone ID Related Accession (Same CDS sequence)	NM_166137.2 , NM_001299546.1
Accession Version	NM_001299546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCTG TGCTGCCGGA AAACGCGGCC GAGATCGCGG TGGCCAATGC AATCAATAAC 
ATCAACGGGA ATGGAAATGG GAACGGGCGC AATCGGGTGC TACCTGGCGG ATTAGGTGGT 
GGTGGCCAGA ACAACATGAT GAATATGCGG GATCGACTGT TCCATGCCAT ATACTTTCGG 
GCGGCGGCCA CCTATGCGGA GTTGGTTCCA AGGAAGGTGC AGTTGACCAT TGAGTTCCTG 
CTGCTGGCCA AGGCCTTGCT GTTCTTCTTT ACGCTCATCT ACGTGCACAA CTCCTTCATC 
AAGAATCCCT GCACTTGCCT GCAGGATGTT AAGAACTGGC CGCGCGAAGG CGTCATCAGA 
GTGGAAATTA TTCCTCACCT GGCGGAGAAG CGTGCAATTT GGCAGGGAAT TAAAAGGGAC 
CAAGAAATAG TGCGCTCCCT CAAGGATTCC TACTACTATG GTATTGGACC CCAAACTCGT 
CAAAATCACG AAACGCTTAA ACGGTACGAG TCCATTCTGG GAAAACTGGG TCTGCCCGTC 
CGACCAACAT TCGCTTACAG TAACGAATCC CTGTACTACT ACTTTGATGC CATCAACATC 
CTGGACACCT ACGACCATCC AAATGCAATA CAGCTCAAGA ACGAAGAGGA TGATGAAGAG 
CAGTACATTG TGGAGTATTC ATTGGAATAC GGCCACCTCC GCTTGTCTTC ATCCACGCGA 
AAGCGTCTAC GCATACCCGT ACTCACTGTC CAACTGGATC CCAATACGGA CAAGTGTTTT 
GGGGACAGTT TGACGCGATA TCTGCTCAAA CGTTTGCTAG GCTATGATGA CTTACTAATG 
GCATCCGTAC GAACAATAGC CGAGCAGGAG GAAAATAAGG GCTACCTGCG CAATGTTATA 
ACCGGCGAAC ATTATCGCTT CGTCTCCATG TGGTGGGCAG CCTGGAGCAG TTACCCGGCT 
GCCTTCTGTG TTATGTTACT ATTCACGTTC TCAGTGTCGA TGTTGCTGCG CTACTCACAC 
CACCAGATCT TTGTGTTTAT AGTCGACCTG CTGCAGATGC TGGAGTACAA TGTGTCTGCC 
CGGTTCCCAA TCGCACCGTT GCTCACTGTC ATATTGGCGC TCGTAGGAAT GGAAGCCATC 
ATGTCGGAGT TCTTTAATGA CACCACTACG GCCTTCTACA TCATACTGAT TGTCTGGATC 
GCGGATCAAT ACGATGCCAT CTGCTGTCAC ACGAGCATCA CCAAGCGTCA TTGGCTCAGA 
TTCTTCTACC TGTACCATTT TGCGTTCTAT GCGTATCACT ATCGCTTCAG TGGACAGTAC 
AGGACGCTGG CCCTGCTCTC GTCGTACCTA TTCACCCAGC ACTCGATGGT GTTCTTCTTT 
CATCGCTACG AGCTGCCAGC CATCATGGCC CAGCACCAGG TCTTCATCAT TACACGCAAC 
CAGGGAGCAG CAGCAGCAGC TGCTGCAGCG GCAGCGGGCG CGGGCGGAGC AGAGGCAGCA 
GGAGGGGGCG CAGGAGCAGG CGCTGCAGCT GGTGGACAGC AAGGAGATGG TGGGGCAGGA 
GGAGGCGGCA TCGGTGCTGG CCAGCAGGCG TCCATGCAAC GTGCCCGTCT CATAGTCACT 
CGGCTCTTCA GTCAACTTGC AGCACAACGC CATCAGCAGG AACCACGCCC GCCAGGACCC 
ACTGCCGCCG TGGGCGTGGC CTTGGCCAAC CTGAGAAGAC TGCCCGTGGT GGGCCAGTTG 
TTGCAGGGCC GCGAGCAATT CGCCACCGTG CGTCGCGTCT TCCTGGGACA TGGCGGCAAC 
GCAAGGCTGA TGCAGGTGCG TCTGCAGCGT ATTAACGTCG CTGACATACC TGGCTTGCGC 
AACCAGGTGG CAGCGGCCAT GGCAGCAGCG GGGGCCGCCC AAAGGGGCCA GAGCGCTGCA 
ACAGATGAAC CCACCATCGG CGATGTTGCA ACTACAGATG TGGCAGCAGC AGCAGACGCG 
GCAGCAACGC CAGCAGCTAA CGATGCAGCA GATGTCGAAA CAAATCAGGG ACGCAAGCAG 
CCGACAACAT CGAGCATAAA TGATGCAGTA GCAACGGGAG CGGAAGGAGC TCAGGACAAG 
CCCGGCCAGG AGAAAGTGGA GGTGGTGGAG AATTCTGGGC AAGAAAACCT ACAGCATGGC 
GATGGGGATG GGCAAATAGC GGGCATGTCC AAGACGCTGG AAAATCTAAA TTTCGTTGCG 
GCGGATATTA TAAATATGTC GGATGAAACT ACTGTAGCAT CATCGCCCCC AACGAACGGC 
ACTCAACAAC CAGATCAGAC CCTGATCCAC ACCCACGTCC ATGTCCATCA GACTCAGCCA 
AAGGCGAGGC AATTAGAAAA TAATTTGCCA GAGTTGCTGA AAGCTGAGGG GGGGGCATCC 
GCAGCAACGG CATCAGGTGA AAAGCAAAGC TGCCCACTAC AAGCACCCAG CATTATGAAT 
GACCAGCAGC AGCAGCAGCA GCAGCAGCAG AGGCAGAAAA ACAAACCGGA CGAGCCCGCG 
ACAGCAACGG TAGCAACATC AGCAGTTGCC ACGTCAAAAC AATCCTCTGG GCCAGGAGCA 
ACAGAGGTAG GAGTGGTAGG AGCGGCAGGA GGAGCCGAGG CAACAGCCAG GCCAATGATG 
GATGACAGGC AAACAGTTGA TTTTATAGAA GGCGACAGCC CAACGTCAGC AGCAAATTGC 
CAACTCAATG CTGGCGCAAA CCACAAGGCA CAGGAGCAGC AGCTAATGAA AACTTCACAA 
CATACATCAA ACCAAGTGAC AACAGCAGCA GCAACAGGAG CAGCAGCAGG AACACCAGCA 
ACAGTAACAC AAGCAACTGG AGCAACAGCC GCAATGGGAA CAGCAGAAAC ATCAGCAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286475.1
CDS	147..3029
Translation	MAAVLPENAAEIAVANAINNINGNGNGNGRNRVLPGGLGGGGQNNMMNMRDRLFHAIYFRAAATYAELVPRKVQLTIEFLLLAKALLFFFTLIYVHNSFIKNPCTCLQDVKNWPREGVIRVEIIPHLAEKRAIWQGIKRDQEIVRSLKDSYYYGIGPQTRQNHETLKRYESILGKLGLPVRPTFAYSNESLYYYFDAINILDTYDHPNAIQLKNEEDDEEQYIVEYSLEYGHLRLSSSTRKRLRIPVLTVQLDPNTDKCFGDSLTRYLLKRLLGYDDLLMASVRTIAEQEENKGYLRNVITGEHYRFVSMWWAAWSSYPAAFCVMLLFTFSVSMLLRYSHHQIFVFIVDLLQMLEYNVSARFPIAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWIADQYDAICCHTSITKRHWLRFFYLYHFAFYAYHYRFSGQYRTLALLSSYLFTQHSMVFFFHRYELPAIMAQHQVFIITRNQGAAAAAAAAAAGAGGAEAAGGGAGAGAAAGGQQGDGGAGGGGIGAGQQASMQRARLIVTRLFSQLAAQRHQQEPRPPGPTAAVGVALANLRRLPVVGQLLQGREQFATVRRVFLGHGGNARLMQVRLQRINVADIPGLRNQVAAAMAAAGAAQRGQSAATDEPTIGDVATTDVAAAADAAATPAANDAADVETNQGRKQPTTSSINDAVATGAEGAQDKPGQEKVEVVENSGQENLQHGDGDGQIAGMSKTLENLNFVAADIINMSDETTVASSPPTNGTQQPDQTLIHTHVHVHQTQPKARQLENNLPELLKAEGGASAATASGEKQSCPLQAPSIMNDQQQQQQQQQRQKNKPDEPATATVATSAVATSKQSSGPGATEVGVVGAAGGAEATARPMMDDRQTVDFIEGDSPTSAANCQLNAGANHKAQEQQLMKTSQHTSNQVTTAAATGAAAGTPATVTQATGATAAMGTAETSAE

Target ORF information:

RefSeq Version	NM_001299546.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8405), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299546.1

ORF Insert Sequence:

ATGGCCGCTG TGCTGCCGGA AAACGCGGCC GAGATCGCGG TGGCCAATGC AATCAATAAC 
ATCAACGGGA ATGGAAATGG GAACGGGCGC AATCGGGTGC TACCTGGCGG ATTAGGTGGT 
GGTGGCCAGA ACAACATGAT GAATATGCGG GATCGACTGT TCCATGCCAT ATACTTTCGG 
GCGGCGGCCA CCTATGCGGA GTTGGTTCCA AGGAAGGTGC AGTTGACCAT TGAGTTCCTG 
CTGCTGGCCA AGGCCTTGCT GTTCTTCTTT ACGCTCATCT ACGTGCACAA CTCCTTCATC 
AAGAATCCCT GCACTTGCCT GCAGGATGTT AAGAACTGGC CGCGCGAAGG CGTCATCAGA 
GTGGAAATTA TTCCTCACCT GGCGGAGAAG CGTGCAATTT GGCAGGGAAT TAAAAGGGAC 
CAAGAAATAG TGCGCTCCCT CAAGGATTCC TACTACTATG GTATTGGACC CCAAACTCGT 
CAAAATCACG AAACGCTTAA ACGGTACGAG TCCATTCTGG GAAAACTGGG TCTGCCCGTC 
CGACCAACAT TCGCTTACAG TAACGAATCC CTGTACTACT ACTTTGATGC CATCAACATC 
CTGGACACCT ACGACCATCC AAATGCAATA CAGCTCAAGA ACGAAGAGGA TGATGAAGAG 
CAGTACATTG TGGAGTATTC ATTGGAATAC GGCCACCTCC GCTTGTCTTC ATCCACGCGA 
AAGCGTCTAC GCATACCCGT ACTCACTGTC CAACTGGATC CCAATACGGA CAAGTGTTTT 
GGGGACAGTT TGACGCGATA TCTGCTCAAA CGTTTGCTAG GCTATGATGA CTTACTAATG 
GCATCCGTAC GAACAATAGC CGAGCAGGAG GAAAATAAGG GCTACCTGCG CAATGTTATA 
ACCGGCGAAC ATTATCGCTT CGTCTCCATG TGGTGGGCAG CCTGGAGCAG TTACCCGGCT 
GCCTTCTGTG TTATGTTACT ATTCACGTTC TCAGTGTCGA TGTTGCTGCG CTACTCACAC 
CACCAGATCT TTGTGTTTAT AGTCGACCTG CTGCAGATGC TGGAGTACAA TGTGTCTGCC 
CGGTTCCCAA TCGCACCGTT GCTCACTGTC ATATTGGCGC TCGTAGGAAT GGAAGCCATC 
ATGTCGGAGT TCTTTAATGA CACCACTACG GCCTTCTACA TCATACTGAT TGTCTGGATC 
GCGGATCAAT ACGATGCCAT CTGCTGTCAC ACGAGCATCA CCAAGCGTCA TTGGCTCAGA 
TTCTTCTACC TGTACCATTT TGCGTTCTAT GCGTATCACT ATCGCTTCAG TGGACAGTAC 
AGGACGCTGG CCCTGCTCTC GTCGTACCTA TTCACCCAGC ACTCGATGGT GTTCTTCTTT 
CATCGCTACG AGCTGCCAGC CATCATGGCC CAGCACCAGG TCTTCATCAT TACACGCAAC 
CAGGGAGCAG CAGCAGCAGC TGCTGCAGCG GCAGCGGGCG CGGGCGGAGC AGAGGCAGCA 
GGAGGGGGCG CAGGAGCAGG CGCTGCAGCT GGTGGACAGC AAGGAGATGG TGGGGCAGGA 
GGAGGCGGCA TCGGTGCTGG CCAGCAGGCG TCCATGCAAC GTGCCCGTCT CATAGTCACT 
CGGCTCTTCA GTCAACTTGC AGCACAACGC CATCAGCAGG AACCACGCCC GCCAGGACCC 
ACTGCCGCCG TGGGCGTGGC CTTGGCCAAC CTGAGAAGAC TGCCCGTGGT GGGCCAGTTG 
TTGCAGGGCC GCGAGCAATT CGCCACCGTG CGTCGCGTCT TCCTGGGACA TGGCGGCAAC 
GCAAGGCTGA TGCAGGTGCG TCTGCAGCGT ATTAACGTCG CTGACATACC TGGCTTGCGC 
AACCAGGTGG CAGCGGCCAT GGCAGCAGCG GGGGCCGCCC AAAGGGGCCA GAGCGCTGCA 
ACAGATGAAC CCACCATCGG CGATGTTGCA ACTACAGATG TGGCAGCAGC AGCAGACGCG 
GCAGCAACGC CAGCAGCTAA CGATGCAGCA GATGTCGAAA CAAATCAGGG ACGCAAGCAG 
CCGACAACAT CGAGCATAAA TGATGCAGTA GCAACGGGAG CGGAAGGAGC TCAGGACAAG 
CCCGGCCAGG AGAAAGTGGA GGTGGTGGAG AATTCTGGGC AAGAAAACCT ACAGCATGGC 
GATGGGGATG GGCAAATAGC GGGCATGTCC AAGACGCTGG AAAATCTAAA TTTCGTTGCG 
GCGGATATTA TAAATATGTC GGATGAAACT ACTGTAGCAT CATCGCCCCC AACGAACGGC 
ACTCAACAAC CAGATCAGAC CCTGATCCAC ACCCACGTCC ATGTCCATCA GACTCAGCCA 
AAGGCGAGGC AATTAGAAAA TAATTTGCCA GAGTTGCTGA AAGCTGAGGG GGGGGCATCC 
GCAGCAACGG CATCAGGTGA AAAGCAAAGC TGCCCACTAC AAGCACCCAG CATTATGAAT 
GACCAGCAGC AGCAGCAGCA GCAGCAGCAG AGGCAGAAAA ACAAACCGGA CGAGCCCGCG 
ACAGCAACGG TAGCAACATC AGCAGTTGCC ACGTCAAAAC AATCCTCTGG GCCAGGAGCA 
ACAGAGGTAG GAGTGGTAGG AGCGGCAGGA GGAGCCGAGG CAACAGCCAG GCCAATGATG 
GATGACAGGC AAACAGTTGA TTTTATAGAA GGCGACAGCC CAACGTCAGC AGCAAATTGC 
CAACTCAATG CTGGCGCAAA CCACAAGGCA CAGGAGCAGC AGCTAATGAA AACTTCACAA 
CATACATCAA ACCAAGTGAC AACAGCAGCA GCAACAGGAG CAGCAGCAGG AACACCAGCA 
ACAGTAACAC AAGCAACTGG AGCAACAGCC GCAATGGGAA CAGCAGAAAC ATCAGCAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8405 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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