Mlf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlf
Entrez Gene ID	36750
Full Name	Myelodysplasia/myeloid leukemia factor
Synonyms	BcDNA:SD18148,CG8295,Dmel\CG8295,MLF,dMLF,dmlf,mlf
General protein information	Preferred Names Myelodysplasia/myeloid leukemia factor Names CG8295 gene product from transcript CG8295-RD CG8295-PA CG8295-PB CG8295-PC CG8295-PD CG8295-PE CG8295-PF CG8295-PG CG8295-PH Mlf-PA Mlf-PB Mlf-PC Mlf-PD Mlf-PE Mlf-PF Mlf-PG Mlf-PH drosophila myeloid leukemia factor myeloid leukemia factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52D2-52D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079029.5	NP_523753.1	Myelodysplasia/myeloid leukemia factor, isoform A
NM_166123.4	NP_725502.1	Myelodysplasia/myeloid leukemia factor, isoform B
NM_001259426.2	NP_001246355.1	Myelodysplasia/myeloid leukemia factor, isoform H
NM_001259425.1	NP_001246354.1	Myelodysplasia/myeloid leukemia factor, isoform G
NM_166122.4	NP_725501.2	Myelodysplasia/myeloid leukemia factor, isoform C
NM_001259424.2	NP_001246353.1	Myelodysplasia/myeloid leukemia factor, isoform F
NM_206125.2	NP_995847.1	Myelodysplasia/myeloid leukemia factor, isoform D
NM_001169693.1	NP_001163164.1	Myelodysplasia/myeloid leukemia factor, isoform E

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Mlf	NP_523753.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011838	XP_320679.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data indicate that the MLF/DnaJ-1-dependent increase in Lz level allows the repression of Notch expression and signaling to prevent aberrant blood cell development. Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo.
Title: Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.

mlf participates in a positive feedback loop to fine tune the activity of the RUNX transcription factor Lozenge (LZ) during development of the crystal cells.
Title: Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis.

Data revealed that MLF interacts with CSN3 in vivo and that the PCI domain of the dCSN3 protein is sufficient for this to occur, possibly functioning as a structural scaffold for assembly of the COP9 signalosome complex.
Title: The myeloid leukemia factor interacts with COP9 signalosome subunit 3 in Drosophila melanogaster.

MLF1 has been described as mainly cytoplasmic, but in Drosophila, one of the dMLF isoforms (dMLFA) localized mainly in the nucleus.
Title: Identification of novel nuclear localization signals of Drosophila myeloid leukemia factor.

Mlf and its mutant were characterized. Mlf can act developmentaly and post-developmentally to suppress neurodegeneration and premature aging in a cerebellar ataxia model.
Title: Characterization of the Drosophila myeloid leukemia factor.

The following Mlf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10131	NM_079029.5 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10132	NM_206125.2 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10133	NM_166123.4 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa10134	NM_166122.4 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12285	NM_001259424.2 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa12286	NM_001169693.1 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12286	NM_001259426.2 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12299	NM_001259425.1 Latest version!	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlf ( NM_079029.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523753.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10131
Clone ID Related Accession (Same CDS sequence)	NM_079029.5
Accession Version	NM_079029.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079029.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGC ATACATCCAC ATCCCTATGC CGCCAATCCG 
AGGCGCCAAC AACGTGCCGT AAAACATTTT CACACGGAGG ACGAAGCGGC CAGCTCCTAC 
AAGGCGGTCA AGCGCGGCAA GAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523753.1
CDS	103..1032
Translation	MSLFGALMGDFDDDLGLMNNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSIHPHPYAANPRRQQRAVKHFHTEDEAASSYKAVKRGKKK

Target ORF information:

RefSeq Version	NM_079029.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079029.5

ORF Insert Sequence:

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGC ATACATCCAC ATCCCTATGC CGCCAATCCG 
AGGCGCCAAC AACGTGCCGT AAAACATTTT CACACGGAGG ACGAAGCGGC CAGCTCCTAC 
AAGGCGGTCA AGCGCGGCAA GAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_206125.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995847.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa10132
Clone ID Related Accession (Same CDS sequence)	NM_206125.2
Accession Version	NM_206125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_206125.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG CATACATCCA 
CATCCCTATG CCGCCAATCC GAGGCGCCAA CAACGTGCCG TAAAACATTT TCACACGGAG 
GACGAAGCGG CCAGCTCCTA CAAGGCGGTC AAGCGCGGCA AGAAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995847.1
CDS	109..1239
Translation	MSLFGALMGDFDDDLGLMNNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSGNIATAAAPTPTSSPTTYDTSNGNNNNYVSSRRSYLRNGHGHSLATPRRPLRTPPSSPLATVSTSPSIHPHPYAANPRRQQRAVKHFHTEDEAASSYKAVKRGKKK

Target ORF information:

RefSeq Version	NM_206125.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206125.2

ORF Insert Sequence:

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG CATACATCCA 
CATCCCTATG CCGCCAATCC GAGGCGCCAA CAACGTGCCG TAAAACATTT TCACACGGAG 
GACGAAGCGG CCAGCTCCTA CAAGGCGGTC AAGCGCGGCA AGAAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_166123.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725502.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10133
Clone ID Related Accession (Same CDS sequence)	NM_166123.4
Accession Version	NM_166123.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_166123.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGA TATAATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725502.1
CDS	109..930
Translation	MSLFGALMGDFDDDLGLMNNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNRYNY

Target ORF information:

RefSeq Version	NM_166123.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166123.4

ORF Insert Sequence:

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGA TATAATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_166122.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725501.2 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10134
Clone ID Related Accession (Same CDS sequence)	NM_166122.4
Accession Version	NM_166122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166122.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACAAA AACGTAAAAG CAAATCAAAC CGTTCACAAA TGGAGCTCCA CAACCACATG 
AACCACACTA TGAACGCGAT GAACATGCAG ATGCGCTCGA TGAATCGCCT GATGAACAGC 
TTTATGCCCG ATCCCTTCAT GCAGGTCTCG CCCTTTGACC AGGGATTCCA GCAGAACGCT 
CTCATGGAGC GTCCGCAGAT GCCGGCCATG CCAGCCATGG GCCTCTTCGG CATGCCCATG 
ATGCCAAACT TTAATCGCCT GTTGAACGCT GATATTGGTG GCAATTCAGG CGCATCCTTC 
TGCCAGAGCA CCGTGATGAC CATGTCATCG GGTCCCGATG GGCGTCCTCA GATCTACCAG 
GCCAGCACTA GTACCAAAAC AGGACCGGGA GGCGTTCGTG AGACCCGCAG GACGGTGCAG 
GACTCGCGCA CTGGGGTGAA GAAGATGGCC ATTGGTCATC ACATCGGCGA GCGGGCACAC 
ATTATTGAGA AAGAGCAGGA CATGCGCTCA GGACAACTGG AGGAGCGCCA GGAGTTCATT 
AATCTGGAGG AGGGAGAAGC CGAGCAGTTT GACAGGGAGT TTACATCGCG CGCTAGTCGC 
GGAGCGGTGC AGTCAAGACA TCATGCTGGT GGCATGCAGG CCATCATGCC CGCCCGTCCA 
GCGGCACACA CCTCGACGTT GACCATTGAG CCAGTGGAGG ACGACGACGA CGATGATGAT 
GACTGTGTAA TCCAGGAGCA GCAACCGGTT CGCTCCTCCG CGGGCCGCCA TTATTCCAGT 
GCGCCAACGG CACCGCAGAA CAGTGGCAAC ATTGCAACAG CAGCAGCACC AACACCAACA 
TCCTCGCCAA CAACCTACGA TACAAGCAAC GGAAACAACA ATAACTACGT GAGCTCTCGT 
CGTTCGTACC TGCGAAATGG GCATGGGCAT AGCCTGGCCA CGCCGCGCCG CCCACTAAGG 
ACACCCCCAT CCTCGCCGCT GGCTACGGTG TCCACTTCTC CAAGCATACA TCCACATCCC 
TATGCCGCCA ATCCGAGGCG CCAACAACGT GCCGTAAAAC ATTTTCACAC GGAGGACGAA 
GCGGCCAGCT CCTACAAGGC GGTCAAGCGC GGCAAGAAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725501.2
CDS	92..1216
Translation	MKQKRKSKSNRSQMELHNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSGNIATAAAPTPTSSPTTYDTSNGNNNNYVSSRRSYLRNGHGHSLATPRRPLRTPPSSPLATVSTSPSIHPHPYAANPRRQQRAVKHFHTEDEAASSYKAVKRGKKK

Target ORF information:

RefSeq Version	NM_166122.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166122.4

ORF Insert Sequence:

ATGAAACAAA AACGTAAAAG CAAATCAAAC CGTTCACAAA TGGAGCTCCA CAACCACATG 
AACCACACTA TGAACGCGAT GAACATGCAG ATGCGCTCGA TGAATCGCCT GATGAACAGC 
TTTATGCCCG ATCCCTTCAT GCAGGTCTCG CCCTTTGACC AGGGATTCCA GCAGAACGCT 
CTCATGGAGC GTCCGCAGAT GCCGGCCATG CCAGCCATGG GCCTCTTCGG CATGCCCATG 
ATGCCAAACT TTAATCGCCT GTTGAACGCT GATATTGGTG GCAATTCAGG CGCATCCTTC 
TGCCAGAGCA CCGTGATGAC CATGTCATCG GGTCCCGATG GGCGTCCTCA GATCTACCAG 
GCCAGCACTA GTACCAAAAC AGGACCGGGA GGCGTTCGTG AGACCCGCAG GACGGTGCAG 
GACTCGCGCA CTGGGGTGAA GAAGATGGCC ATTGGTCATC ACATCGGCGA GCGGGCACAC 
ATTATTGAGA AAGAGCAGGA CATGCGCTCA GGACAACTGG AGGAGCGCCA GGAGTTCATT 
AATCTGGAGG AGGGAGAAGC CGAGCAGTTT GACAGGGAGT TTACATCGCG CGCTAGTCGC 
GGAGCGGTGC AGTCAAGACA TCATGCTGGT GGCATGCAGG CCATCATGCC CGCCCGTCCA 
GCGGCACACA CCTCGACGTT GACCATTGAG CCAGTGGAGG ACGACGACGA CGATGATGAT 
GACTGTGTAA TCCAGGAGCA GCAACCGGTT CGCTCCTCCG CGGGCCGCCA TTATTCCAGT 
GCGCCAACGG CACCGCAGAA CAGTGGCAAC ATTGCAACAG CAGCAGCACC AACACCAACA 
TCCTCGCCAA CAACCTACGA TACAAGCAAC GGAAACAACA ATAACTACGT GAGCTCTCGT 
CGTTCGTACC TGCGAAATGG GCATGGGCAT AGCCTGGCCA CGCCGCGCCG CCCACTAAGG 
ACACCCCCAT CCTCGCCGCT GGCTACGGTG TCCACTTCTC CAAGCATACA TCCACATCCC 
TATGCCGCCA ATCCGAGGCG CCAACAACGT GCCGTAAAAC ATTTTCACAC GGAGGACGAA 
GCGGCCAGCT CCTACAAGGC GGTCAAGCGC GGCAAGAAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_001259424.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246353.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa12285
Clone ID Related Accession (Same CDS sequence)	NM_001259424.2
Accession Version	NM_001259424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259424.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACAAA AACGTAAAAG CAAATCAAAC CGTTCACAAA TGGAGCTCCA CAACCACATG 
AACCACACTA TGAACGCGAT GAACATGCAG ATGCGCTCGA TGAATCGCCT GATGAACAGC 
TTTATGCCCG ATCCCTTCAT GCAGGTCTCG CCCTTTGACC AGGGATTCCA GCAGAACGCT 
CTCATGGAGC GTCCGCAGAT GCCGGCCATG CCAGCCATGG GCCTCTTCGG CATGCCCATG 
ATGCCAAACT TTAATCGCCT GTTGAACGCT GATATTGGTG GCAATTCAGG CGCATCCTTC 
TGCCAGAGCA CCGTGATGAC CATGTCATCG GGTCCCGATG GGCGTCCTCA GATCTACCAG 
GCCAGCACTA GTACCAAAAC AGGACCGGGA GGCGTTCGTG AGACCCGCAG GACGGTGCAG 
GACTCGCGCA CTGGGGTGAA GAAGATGGCC ATTGGTCATC ACATCGGCGA GCGGGCACAC 
ATTATTGAGA AAGAGCAGGA CATGCGCTCA GGACAACTGG AGGAGCGCCA GGAGTTCATT 
AATCTGGAGG AGGGAGAAGC CGAGCAGTTT GACAGGGAGT TTACATCGCG CGCTAGTCGC 
GGAGCGGTGC AGTCAAGACA TCATGCTGGT GGCATGCAGG CCATCATGCC CGCCCGTCCA 
GCGGCACACA CCTCGACGTT GACCATTGAG CCAGTGGAGG ACGACGACGA CGATGATGAT 
GACTGTGTAA TCCAGGAGCA GCAACCGGTT CGCTCCTCCG CGGGCCGCCA TTATTCCAGT 
GCGCCAACGG CACCGCAGAA CAGCAGCATA CATCCACATC CCTATGCCGC CAATCCGAGG 
CGCCAACAAC GTGCCGTAAA ACATTTTCAC ACGGAGGACG AAGCGGCCAG CTCCTACAAG 
GCGGTCAAGC GCGGCAAGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246353.1
CDS	92..1018
Translation	MKQKRKSKSNRSQMELHNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSSIHPHPYAANPRRQQRAVKHFHTEDEAASSYKAVKRGKKK

Target ORF information:

RefSeq Version	NM_001259424.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259424.2

ORF Insert Sequence:

ATGAAACAAA AACGTAAAAG CAAATCAAAC CGTTCACAAA TGGAGCTCCA CAACCACATG 
AACCACACTA TGAACGCGAT GAACATGCAG ATGCGCTCGA TGAATCGCCT GATGAACAGC 
TTTATGCCCG ATCCCTTCAT GCAGGTCTCG CCCTTTGACC AGGGATTCCA GCAGAACGCT 
CTCATGGAGC GTCCGCAGAT GCCGGCCATG CCAGCCATGG GCCTCTTCGG CATGCCCATG 
ATGCCAAACT TTAATCGCCT GTTGAACGCT GATATTGGTG GCAATTCAGG CGCATCCTTC 
TGCCAGAGCA CCGTGATGAC CATGTCATCG GGTCCCGATG GGCGTCCTCA GATCTACCAG 
GCCAGCACTA GTACCAAAAC AGGACCGGGA GGCGTTCGTG AGACCCGCAG GACGGTGCAG 
GACTCGCGCA CTGGGGTGAA GAAGATGGCC ATTGGTCATC ACATCGGCGA GCGGGCACAC 
ATTATTGAGA AAGAGCAGGA CATGCGCTCA GGACAACTGG AGGAGCGCCA GGAGTTCATT 
AATCTGGAGG AGGGAGAAGC CGAGCAGTTT GACAGGGAGT TTACATCGCG CGCTAGTCGC 
GGAGCGGTGC AGTCAAGACA TCATGCTGGT GGCATGCAGG CCATCATGCC CGCCCGTCCA 
GCGGCACACA CCTCGACGTT GACCATTGAG CCAGTGGAGG ACGACGACGA CGATGATGAT 
GACTGTGTAA TCCAGGAGCA GCAACCGGTT CGCTCCTCCG CGGGCCGCCA TTATTCCAGT 
GCGCCAACGG CACCGCAGAA CAGCAGCATA CATCCACATC CCTATGCCGC CAATCCGAGG 
CGCCAACAAC GTGCCGTAAA ACATTTTCAC ACGGAGGACG AAGCGGCCAG CTCCTACAAG 
GCGGTCAAGC GCGGCAAGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_001259426.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246355.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa12286
Clone ID Related Accession (Same CDS sequence)	NM_001259426.2 , NM_001169693.1
Accession Version	NM_001259426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259426.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG AGATTGGCGT 
ATTTATAATT CGCGGTACCA CCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246355.1
CDS	103..1152
Translation	MSLFGALMGDFDDDLGLMNNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSGNIATAAAPTPTSSPTTYDTSNGNNNNYVSSRRSYLRNGHGHSLATPRRPLRTPPSSPLATVSTSPRDWRIYNSRYHRK

Target ORF information:

RefSeq Version	NM_001259426.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259426.2

ORF Insert Sequence:

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG AGATTGGCGT 
ATTTATAATT CGCGGTACCA CCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_001169693.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163164.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa12286
Clone ID Related Accession (Same CDS sequence)	NM_001259426.2 , NM_001169693.1
Accession Version	NM_001169693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG AGATTGGCGT 
ATTTATAATT CGCGGTACCA CCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163164.1
CDS	109..1158
Translation	MSLFGALMGDFDDDLGLMNNHMNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSGNIATAAAPTPTSSPTTYDTSNGNNNNYVSSRRSYLRNGHGHSLATPRRPLRTPPSSPLATVSTSPRDWRIYNSRYHRK

Target ORF information:

RefSeq Version	NM_001169693.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169693.1

ORF Insert Sequence:

ATGTCTTTAT TCGGAGCGTT GATGGGTGAT TTCGACGACG ATCTCGGCCT TATGAACAAC 
CACATGAACC ACACTATGAA CGCGATGAAC ATGCAGATGC GCTCGATGAA TCGCCTGATG 
AACAGCTTTA TGCCCGATCC CTTCATGCAG GTCTCGCCCT TTGACCAGGG ATTCCAGCAG 
AACGCTCTCA TGGAGCGTCC GCAGATGCCG GCCATGCCAG CCATGGGCCT CTTCGGCATG 
CCCATGATGC CAAACTTTAA TCGCCTGTTG AACGCTGATA TTGGTGGCAA TTCAGGCGCA 
TCCTTCTGCC AGAGCACCGT GATGACCATG TCATCGGGTC CCGATGGGCG TCCTCAGATC 
TACCAGGCCA GCACTAGTAC CAAAACAGGA CCGGGAGGCG TTCGTGAGAC CCGCAGGACG 
GTGCAGGACT CGCGCACTGG GGTGAAGAAG ATGGCCATTG GTCATCACAT CGGCGAGCGG 
GCACACATTA TTGAGAAAGA GCAGGACATG CGCTCAGGAC AACTGGAGGA GCGCCAGGAG 
TTCATTAATC TGGAGGAGGG AGAAGCCGAG CAGTTTGACA GGGAGTTTAC ATCGCGCGCT 
AGTCGCGGAG CGGTGCAGTC AAGACATCAT GCTGGTGGCA TGCAGGCCAT CATGCCCGCC 
CGTCCAGCGG CACACACCTC GACGTTGACC ATTGAGCCAG TGGAGGACGA CGACGACGAT 
GATGATGACT GTGTAATCCA GGAGCAGCAA CCGGTTCGCT CCTCCGCGGG CCGCCATTAT 
TCCAGTGCGC CAACGGCACC GCAGAACAGT GGCAACATTG CAACAGCAGC AGCACCAACA 
CCAACATCCT CGCCAACAAC CTACGATACA AGCAACGGAA ACAACAATAA CTACGTGAGC 
TCTCGTCGTT CGTACCTGCG AAATGGGCAT GGGCATAGCC TGGCCACGCC GCGCCGCCCA 
CTAAGGACAC CCCCATCCTC GCCGCTGGCT ACGGTGTCCA CTTCTCCAAG AGATTGGCGT 
ATTTATAATT CGCGGTACCA CCGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlf ( NM_001259425.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246354.1 Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa12299
Clone ID Related Accession (Same CDS sequence)	NM_001259425.1
Accession Version	NM_001259425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Myelodysplasia/myeloid leukemia factor, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCACA CTATGAACGC GATGAACATG CAGATGCGCT CGATGAATCG CCTGATGAAC 
AGCTTTATGC CCGATCCCTT CATGCAGGTC TCGCCCTTTG ACCAGGGATT CCAGCAGAAC 
GCTCTCATGG AGCGTCCGCA GATGCCGGCC ATGCCAGCCA TGGGCCTCTT CGGCATGCCC 
ATGATGCCAA ACTTTAATCG CCTGTTGAAC GCTGATATTG GTGGCAATTC AGGCGCATCC 
TTCTGCCAGA GCACCGTGAT GACCATGTCA TCGGGTCCCG ATGGGCGTCC TCAGATCTAC 
CAGGCCAGCA CTAGTACCAA AACAGGACCG GGAGGCGTTC GTGAGACCCG CAGGACGGTG 
CAGGACTCGC GCACTGGGGT GAAGAAGATG GCCATTGGTC ATCACATCGG CGAGCGGGCA 
CACATTATTG AGAAAGAGCA GGACATGCGC TCAGGACAAC TGGAGGAGCG CCAGGAGTTC 
ATTAATCTGG AGGAGGGAGA AGCCGAGCAG TTTGACAGGG AGTTTACATC GCGCGCTAGT 
CGCGGAGCGG TGCAGTCAAG ACATCATGCT GGTGGCATGC AGGCCATCAT GCCCGCCCGT 
CCAGCGGCAC ACACCTCGAC GTTGACCATT GAGCCAGTGG AGGACGACGA CGACGATGAT 
GATGACTGTG TAATCCAGGA GCAGCAACCG GTTCGCTCCT CCGCGGGCCG CCATTATTCC 
AGTGCGCCAA CGGCACCGCA GAACAGCATA CATCCACATC CCTATGCCGC CAATCCGAGG 
CGCCAACAAC GTGCCGTAAA ACATTTTCAC ACGGAGGACG AAGCGGCCAG CTCCTACAAG 
GCGGTCAAGC GCGGCAAGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246354.1
CDS	68..934
Translation	MNHTMNAMNMQMRSMNRLMNSFMPDPFMQVSPFDQGFQQNALMERPQMPAMPAMGLFGMPMMPNFNRLLNADIGGNSGASFCQSTVMTMSSGPDGRPQIYQASTSTKTGPGGVRETRRTVQDSRTGVKKMAIGHHIGERAHIIEKEQDMRSGQLEERQEFINLEEGEAEQFDREFTSRASRGAVQSRHHAGGMQAIMPARPAAHTSTLTIEPVEDDDDDDDDCVIQEQQPVRSSAGRHYSSAPTAPQNSIHPHPYAANPRRQQRAVKHFHTEDEAASSYKAVKRGKKK

Target ORF information:

RefSeq Version	NM_001259425.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Myelodysplasia/myeloid leukemia factor (Mlf), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259425.1

ORF Insert Sequence:

ATGAACCACA CTATGAACGC GATGAACATG CAGATGCGCT CGATGAATCG CCTGATGAAC 
AGCTTTATGC CCGATCCCTT CATGCAGGTC TCGCCCTTTG ACCAGGGATT CCAGCAGAAC 
GCTCTCATGG AGCGTCCGCA GATGCCGGCC ATGCCAGCCA TGGGCCTCTT CGGCATGCCC 
ATGATGCCAA ACTTTAATCG CCTGTTGAAC GCTGATATTG GTGGCAATTC AGGCGCATCC 
TTCTGCCAGA GCACCGTGAT GACCATGTCA TCGGGTCCCG ATGGGCGTCC TCAGATCTAC 
CAGGCCAGCA CTAGTACCAA AACAGGACCG GGAGGCGTTC GTGAGACCCG CAGGACGGTG 
CAGGACTCGC GCACTGGGGT GAAGAAGATG GCCATTGGTC ATCACATCGG CGAGCGGGCA 
CACATTATTG AGAAAGAGCA GGACATGCGC TCAGGACAAC TGGAGGAGCG CCAGGAGTTC 
ATTAATCTGG AGGAGGGAGA AGCCGAGCAG TTTGACAGGG AGTTTACATC GCGCGCTAGT 
CGCGGAGCGG TGCAGTCAAG ACATCATGCT GGTGGCATGC AGGCCATCAT GCCCGCCCGT 
CCAGCGGCAC ACACCTCGAC GTTGACCATT GAGCCAGTGG AGGACGACGA CGACGATGAT 
GATGACTGTG TAATCCAGGA GCAGCAACCG GTTCGCTCCT CCGCGGGCCG CCATTATTCC 
AGTGCGCCAA CGGCACCGCA GAACAGCATA CATCCACATC CCTATGCCGC CAATCCGAGG 
CGCCAACAAC GTGCCGTAAA ACATTTTCAC ACGGAGGACG AAGCGGCCAG CTCCTACAAG 
GCGGTCAAGC GCGGCAAGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mlf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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