ckn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ckn
Entrez Gene ID	36673
Full Name	caskin
Synonyms	BcDNA:GM06913,CG12424,Dmel\CG12424
General protein information	Preferred Names caskin Names CG12424 gene product from transcript CG12424-RE CG12424-PB CG12424-PC CG12424-PD CG12424-PE CG12424-PF ckn-PB ckn-PC ckn-PD ckn-PE ckn-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51D6-51D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_166077.2	NP_725434.1	caskin, isoform C
NM_166076.2	NP_725433.1	caskin, isoform B
NM_001299508.1	NP_001286437.1	caskin, isoform D
NM_137165.2	NP_611009.1	caskin, isoform E
NM_001299509.1	NP_001286438.1	caskin, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ckn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ckn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10046	NM_166076.2 Latest version!	Drosophila melanogaster caskin (ckn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa10046	NM_137165.2 Latest version!	Drosophila melanogaster caskin (ckn), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa10046	NM_166077.2 Latest version!	Drosophila melanogaster caskin (ckn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa21812	NM_001299508.1 Latest version!	Drosophila melanogaster caskin (ckn), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa21813	NM_001299509.1 Latest version!	Drosophila melanogaster caskin (ckn), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ckn ( NM_166077.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725434.1 Drosophila melanogaster caskin (ckn), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10046
Clone ID Related Accession (Same CDS sequence)	NM_166077.2 , NM_166076.2 , NM_137165.2
Accession Version	NM_166077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	caskin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166077.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725434.1
CDS	684..3458
Translation	MHRSLKPHASTAQAKKVQPPTIPGDSPQDLHLYQQPQQLHHHHHQHPNHHHPHHQPSIPLYRLSGPLRHHQNHQADDGISMSGSSIVSSPSLEEGGFNLPRETSVALRLKDEVGGTSTAAPTTGGPGGGGATVVGAPPPGAGKPGVAMVPPPPVFCATLREPLTHKAAVYYHQQLALQEDQGIDLTQSPGRDSPGSSSGSAGSGSRHSTASLDSGRASSYLTGVSSSGASIRAPLSSPRCSSVSSCSIGSVDRQRNDELIIDWLLEMKHEEYAQLFIAAGYDLPTIARMTPEDLTAIGIKNPHHRERIKQRIDKLQVLDNLPHFVPGSIEEWLQLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDIISGKWNPGGANAYQQQEYQRKPWQFNVPIPSTPSYVPTTRVSWDDTKIYATSSVLNSTAPSGSSCLNSSLANGDAYYCTGSNHECCSGNDVVLIKVRQPRGKSLESLEDIHDNSTRSHLTFSHYTTTDYGHFGPPTTAAAAAAMAAAATLQQQHHHQQLLHQQQQQAAAARLLGNNAPGIGWRRSYDDGDITPTNDATRELLYEQEGGGTLPRQQRGILQRAVLNTMPPLEHHHYMNAAAAAEYGAASGVEGGANYMGGSPLTGKKIAPEPPRRHCSIRNSRTLSGEGVPSGAAAQAHQQAAQQQQGQHMFYGHTAQHWQQQQQQAGAGTGQQPIYANYATIQQTTAEIHCEKYHDSKSTSSIDSIDTIPFANENTGTIKQRLLNRQELQGGGAGNNNVPGGGHPAHLHSSSSSSSSSSTNTIANIAHSFHSLKSSSSLHDATLARSESMGSTASGGSALHLRSPTLDMSNAGGSTAEPASSPEAAVPTVAINNGVPPKVSVNVLNDIGNMLANLTDELDAMLEEEKRVGLNIDSE

Target ORF information:

RefSeq Version	NM_166077.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster caskin (ckn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166077.2

ORF Insert Sequence:

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckn ( NM_166076.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725433.1 Drosophila melanogaster caskin (ckn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10046
Clone ID Related Accession (Same CDS sequence)	NM_166077.2 , NM_166076.2 , NM_137165.2
Accession Version	NM_166076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	caskin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166076.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725433.1
CDS	366..3140
Translation	MHRSLKPHASTAQAKKVQPPTIPGDSPQDLHLYQQPQQLHHHHHQHPNHHHPHHQPSIPLYRLSGPLRHHQNHQADDGISMSGSSIVSSPSLEEGGFNLPRETSVALRLKDEVGGTSTAAPTTGGPGGGGATVVGAPPPGAGKPGVAMVPPPPVFCATLREPLTHKAAVYYHQQLALQEDQGIDLTQSPGRDSPGSSSGSAGSGSRHSTASLDSGRASSYLTGVSSSGASIRAPLSSPRCSSVSSCSIGSVDRQRNDELIIDWLLEMKHEEYAQLFIAAGYDLPTIARMTPEDLTAIGIKNPHHRERIKQRIDKLQVLDNLPHFVPGSIEEWLQLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDIISGKWNPGGANAYQQQEYQRKPWQFNVPIPSTPSYVPTTRVSWDDTKIYATSSVLNSTAPSGSSCLNSSLANGDAYYCTGSNHECCSGNDVVLIKVRQPRGKSLESLEDIHDNSTRSHLTFSHYTTTDYGHFGPPTTAAAAAAMAAAATLQQQHHHQQLLHQQQQQAAAARLLGNNAPGIGWRRSYDDGDITPTNDATRELLYEQEGGGTLPRQQRGILQRAVLNTMPPLEHHHYMNAAAAAEYGAASGVEGGANYMGGSPLTGKKIAPEPPRRHCSIRNSRTLSGEGVPSGAAAQAHQQAAQQQQGQHMFYGHTAQHWQQQQQQAGAGTGQQPIYANYATIQQTTAEIHCEKYHDSKSTSSIDSIDTIPFANENTGTIKQRLLNRQELQGGGAGNNNVPGGGHPAHLHSSSSSSSSSSTNTIANIAHSFHSLKSSSSLHDATLARSESMGSTASGGSALHLRSPTLDMSNAGGSTAEPASSPEAAVPTVAINNGVPPKVSVNVLNDIGNMLANLTDELDAMLEEEKRVGLNIDSE

Target ORF information:

RefSeq Version	NM_166076.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster caskin (ckn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166076.2

ORF Insert Sequence:

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckn ( NM_137165.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611009.1 Drosophila melanogaster caskin (ckn), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa10046
Clone ID Related Accession (Same CDS sequence)	NM_166077.2 , NM_166076.2 , NM_137165.2
Accession Version	NM_137165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	caskin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137165.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611009.1
CDS	684..3458
Translation	MHRSLKPHASTAQAKKVQPPTIPGDSPQDLHLYQQPQQLHHHHHQHPNHHHPHHQPSIPLYRLSGPLRHHQNHQADDGISMSGSSIVSSPSLEEGGFNLPRETSVALRLKDEVGGTSTAAPTTGGPGGGGATVVGAPPPGAGKPGVAMVPPPPVFCATLREPLTHKAAVYYHQQLALQEDQGIDLTQSPGRDSPGSSSGSAGSGSRHSTASLDSGRASSYLTGVSSSGASIRAPLSSPRCSSVSSCSIGSVDRQRNDELIIDWLLEMKHEEYAQLFIAAGYDLPTIARMTPEDLTAIGIKNPHHRERIKQRIDKLQVLDNLPHFVPGSIEEWLQLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDIISGKWNPGGANAYQQQEYQRKPWQFNVPIPSTPSYVPTTRVSWDDTKIYATSSVLNSTAPSGSSCLNSSLANGDAYYCTGSNHECCSGNDVVLIKVRQPRGKSLESLEDIHDNSTRSHLTFSHYTTTDYGHFGPPTTAAAAAAMAAAATLQQQHHHQQLLHQQQQQAAAARLLGNNAPGIGWRRSYDDGDITPTNDATRELLYEQEGGGTLPRQQRGILQRAVLNTMPPLEHHHYMNAAAAAEYGAASGVEGGANYMGGSPLTGKKIAPEPPRRHCSIRNSRTLSGEGVPSGAAAQAHQQAAQQQQGQHMFYGHTAQHWQQQQQQAGAGTGQQPIYANYATIQQTTAEIHCEKYHDSKSTSSIDSIDTIPFANENTGTIKQRLLNRQELQGGGAGNNNVPGGGHPAHLHSSSSSSSSSSTNTIANIAHSFHSLKSSSSLHDATLARSESMGSTASGGSALHLRSPTLDMSNAGGSTAEPASSPEAAVPTVAINNGVPPKVSVNVLNDIGNMLANLTDELDAMLEEEKRVGLNIDSE

Target ORF information:

RefSeq Version	NM_137165.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster caskin (ckn), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137165.2

ORF Insert Sequence:

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGAGTACCA GCGGAAGCCC TGGCAATTCA ATGTGCCCAT ACCCAGTACG 
CCATCCTATG TGCCAACCAC CCGCGTCTCG TGGGACGACA CGAAAATCTA CGCGACCAGC 
AGTGTCTTGA ATTCCACTGC CCCATCCGGG AGCAGCTGCC TCAATAGCAG CCTTGCCAAC 
GGAGATGCCT ACTACTGCAC GGGCAGTAAC CATGAGTGCT GCTCGGGCAA CGATGTGGTG 
CTCATTAAGG TTCGCCAGCC GCGCGGCAAG AGCCTGGAAT CTTTGGAGGA CATCCACGAC 
AACAGCACGC GCAGCCATCT GACCTTCAGC CATTACACAA CCACGGACTA CGGCCACTTT 
GGGCCGCCGA CAACAGCGGC GGCTGCAGCG GCAATGGCCG CAGCGGCCAC ACTGCAGCAG 
CAGCATCATC ACCAGCAGCT GCTCCACCAG CAACAGCAGC AGGCAGCTGC CGCCCGTCTC 
CTGGGCAACA ATGCGCCTGG CATTGGATGG CGGCGATCCT ACGACGACGG CGACATCACA 
CCCACCAACG ATGCCACGAG GGAGCTGCTC TACGAGCAGG AGGGCGGCGG AACTCTGCCA 
CGCCAGCAGC GTGGCATCCT GCAGCGGGCG GTGTTGAACA CCATGCCGCC ACTGGAGCAC 
CATCACTACA TGAATGCAGC GGCTGCGGCG GAGTACGGTG CGGCCAGCGG TGTGGAGGGT 
GGAGCGAATT ACATGGGCGG CAGTCCTTTG ACGGGCAAGA AAATAGCTCC TGAGCCACCT 
AGACGCCACT GCAGCATACG CAACTCCCGC ACGCTGAGCG GAGAGGGAGT GCCGTCGGGA 
GCGGCTGCGC AGGCGCATCA GCAGGCAGCC CAACAGCAGC AGGGCCAGCA CATGTTCTAC 
GGCCACACGG CGCAGCACTG GCAGCAGCAG CAGCAGCAGG CGGGTGCAGG TACTGGTCAG 
CAGCCGATCT ACGCCAACTA TGCCACCATA CAACAGACAA CGGCGGAGAT ACACTGTGAG 
AAGTATCACG ACAGTAAGTC GACTTCCAGC ATCGATTCGA TTGACACGAT ACCATTTGCC 
AACGAGAACA CTGGGACGAT AAAACAGCGG CTGCTCAACC GCCAGGAGCT GCAGGGCGGC 
GGAGCGGGCA ACAACAATGT ACCCGGCGGC GGCCATCCCG CACACCTGCA CTCCTCCAGC 
TCGAGCTCCA GCTCCTCCTC CACGAACACC ATCGCGAATA TAGCGCACTC GTTCCATTCG 
TTGAAATCTT CCAGTTCGCT GCACGATGCC ACATTGGCGC GGAGTGAAAG CATGGGCAGC 
ACAGCCAGCG GTGGAAGTGC CTTGCACCTG CGTTCACCCA CATTGGATAT GTCAAATGCC 
GGTGGATCAA CGGCGGAGCC TGCAAGTTCG CCAGAAGCAG CCGTACCAAC TGTGGCCATA 
AACAACGGCG TTCCGCCCAA AGTTTCGGTG AACGTTCTGA ACGACATTGG CAATATGCTG 
GCCAATCTCA CGGACGAGCT GGACGCCATG CTGGAGGAGG AGAAGCGAGT GGGCCTAAAC 
ATAGACAGTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckn ( NM_001299508.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286437.1 Drosophila melanogaster caskin (ckn), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21812
Clone ID Related Accession (Same CDS sequence)	NM_001299508.1
Accession Version	NM_001299508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	caskin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGTTCGCCA GCCGCGCGGC AAGAGCCTGG AATCTTTGGA GGACATCCAC 
GACAACAGCA CGCGCAGCCA TCTGACCTTC AGCCATTACA CAACCACGGA CTACGGCCAC 
TTTGGGCCGC CGACAACAGC GGCGGCTGCA GCGGCAATGG CCGCAGCGGC CACACTGCAG 
CAGCAGCATC ATCACCAGCA GCTGCTCCAC CAGCAACAGC AGCAGGCAGC TGCCGCCCGT 
CTCCTGGGCA ACAATGCGCC TGGCATTGGA TGGCGGCGAT CCTACGACGA CGGCGACATC 
ACACCCACCA ACGATGCCAC GAGGGAGCTG CTCTACGAGC AGGAGGGCGG CGGAACTCTG 
CCACGCCAGC AGCGTGGCAT CCTGCAGCGG GCGGTGTTGA ACACCATGCC GCCACTGGAG 
CACCATCACT ACATGAATGC AGCGGCTGCG GCGGAGTACG GTGCGGCCAG CGGTGTGGAG 
GGTGGAGCGA ATTACATGGG CGGCAGTCCT TTGACGGGCA AGAAAATAGC TCCTGAGCCA 
CCTAGACGCC ACTGCAGCAT ACGCAACTCC CGCACGCTGA GCGGAGAGGG AGTGCCGTCG 
GGAGCGGCTG CGCAGGCGCA TCAGCAGGCA GCCCAACAGC AGCAGGGCCA GCACATGTTC 
TACGGCCACA CGGCGCAGCA CTGGCAGCAG CAGCAGCAGC AGGCGGGTGC AGGTACTGGT 
CAGCAGCCGA TCTACGCCAA CTATGCCACC ATACAACAGA CAACGGCGGA GATACACTGT 
GAGAAGTATC ACGACAGTAA GTCGACTTCC AGCATCGATT CGATTGACAC GATACCATTT 
GCCAACGAGA ACACTGGGAC GATAAAACAG CGGCTGCTCA ACCGCCAGGA GCTGCAGGGC 
GGCGGAGCGG GCAACAACAA TGTACCCGGC GGCGGCCATC CCGCACACCT GCACTCCTCC 
AGCTCGAGCT CCAGCTCCTC CTCCACGAAC ACCATCGCGA ATATAGCGCA CTCGTTCCAT 
TCGTTGAAAT CTTCCAGTTC GCTGCACGAT GCCACATTGG CGCGGAGTGA AAGCATGGGC 
AGCACAGCCA GCGGTGGAAG TGCCTTGCAC CTGCGTTCAC CCACATTGGA TATGTCAAAT 
GCCGGTGGAT CAACGGCGGA GCCTGCAAGT TCGCCAGAAG CAGCCGTACC AACTGTGGCC 
ATAAACAACG GCGTTCCGCC CAAAGTTTCG GTGAACGTTC TGAACGACAT TGGCAATATG 
CTGGCCAATC TCACGGACGA GCTGGACGCC ATGCTGGAGG AGGAGAAGCG AGTGGGCCTA 
AACATAGACA GTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286437.1
CDS	684..3221
Translation	MHRSLKPHASTAQAKKVQPPTIPGDSPQDLHLYQQPQQLHHHHHQHPNHHHPHHQPSIPLYRLSGPLRHHQNHQADDGISMSGSSIVSSPSLEEGGFNLPRETSVALRLKDEVGGTSTAAPTTGGPGGGGATVVGAPPPGAGKPGVAMVPPPPVFCATLREPLTHKAAVYYHQQLALQEDQGIDLTQSPGRDSPGSSSGSAGSGSRHSTASLDSGRASSYLTGVSSSGASIRAPLSSPRCSSVSSCSIGSVDRQRNDELIIDWLLEMKHEEYAQLFIAAGYDLPTIARMTPEDLTAIGIKNPHHRERIKQRIDKLQVLDNLPHFVPGSIEEWLQLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDIISGKWNPGGANAYQQQVRQPRGKSLESLEDIHDNSTRSHLTFSHYTTTDYGHFGPPTTAAAAAAMAAAATLQQQHHHQQLLHQQQQQAAAARLLGNNAPGIGWRRSYDDGDITPTNDATRELLYEQEGGGTLPRQQRGILQRAVLNTMPPLEHHHYMNAAAAAEYGAASGVEGGANYMGGSPLTGKKIAPEPPRRHCSIRNSRTLSGEGVPSGAAAQAHQQAAQQQQGQHMFYGHTAQHWQQQQQQAGAGTGQQPIYANYATIQQTTAEIHCEKYHDSKSTSSIDSIDTIPFANENTGTIKQRLLNRQELQGGGAGNNNVPGGGHPAHLHSSSSSSSSSSTNTIANIAHSFHSLKSSSSLHDATLARSESMGSTASGGSALHLRSPTLDMSNAGGSTAEPASSPEAAVPTVAINNGVPPKVSVNVLNDIGNMLANLTDELDAMLEEEKRVGLNIDSE

Target ORF information:

RefSeq Version	NM_001299508.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster caskin (ckn), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299508.1

ORF Insert Sequence:

ATGCATCGTT CGCTGAAGCC GCATGCATCC ACCGCACAGG CTAAGAAGGT GCAACCGCCA 
ACGATACCAG GCGACTCGCC GCAGGATTTG CATCTCTATC AGCAGCCGCA GCAGCTTCAC 
CACCACCACC ATCAACACCC AAACCATCAT CATCCGCACC ACCAGCCATC GATACCGCTC 
TATCGGCTAA GCGGACCACT GCGCCATCAT CAAAACCACC AGGCGGACGA TGGGATCAGC 
ATGAGCGGCA GCAGCATCGT TTCGTCGCCC TCGCTGGAGG AGGGTGGCTT CAATTTGCCG 
CGGGAGACGA GTGTGGCCCT GCGTCTTAAG GATGAAGTGG GTGGCACTTC AACGGCAGCC 
CCAACCACTG GCGGCCCAGG AGGAGGTGGA GCGACGGTGG TGGGAGCACC ACCACCCGGT 
GCCGGAAAGC CCGGCGTAGC CATGGTTCCA CCGCCACCCG TTTTCTGTGC CACTTTGCGC 
GAACCGCTGA CCCACAAAGC GGCCGTCTAC TACCACCAAC AGCTGGCCCT GCAGGAGGAT 
CAGGGCATCG ATCTAACCCA ATCGCCTGGC CGAGACTCGC CGGGCTCGTC GTCGGGCTCG 
GCGGGCTCTG GATCTCGTCA CAGCACCGCC AGCTTGGACT CGGGTCGGGC CTCCTCCTAC 
CTGACGGGCG TGTCCTCGTC GGGCGCCTCG ATTCGTGCTC CGCTCTCCTC GCCGCGCTGC 
AGTTCCGTCA GCTCGTGCTC CATTGGCAGT GTGGATCGGC AGCGCAACGA TGAGCTCATC 
ATCGACTGGC TGCTCGAGAT GAAGCACGAG GAGTACGCGC AGCTGTTCAT CGCCGCGGGC 
TATGATCTGC CCACCATTGC GCGCATGACG CCGGAGGATC TCACCGCCAT TGGCATTAAG 
AATCCGCATC ATCGGGAGCG GATCAAGCAG CGGATCGACA AGCTGCAGGT GCTCGACAAT 
TTGCCGCATT TCGTGCCGGG ATCCATAGAG GAATGGCTGC AGCTGCTGCG TCTGGAGGAG 
TATATACAGC CCCTGCTGGA GCAGAACTAT AAGACGGTGC GCGACGTAAC CCAAGTGACT 
TGGGAGGATC TCGAGGACAT TGGCATTGTC AAGCTGGGCC ACCAAAAGAA AATTCTGCTG 
GCCATCAAGC GTGTGAAGGA CATAATTAGC GGAAAGTGGA ACCCAGGCGG CGCCAATGCC 
TACCAACAGC AGGTTCGCCA GCCGCGCGGC AAGAGCCTGG AATCTTTGGA GGACATCCAC 
GACAACAGCA CGCGCAGCCA TCTGACCTTC AGCCATTACA CAACCACGGA CTACGGCCAC 
TTTGGGCCGC CGACAACAGC GGCGGCTGCA GCGGCAATGG CCGCAGCGGC CACACTGCAG 
CAGCAGCATC ATCACCAGCA GCTGCTCCAC CAGCAACAGC AGCAGGCAGC TGCCGCCCGT 
CTCCTGGGCA ACAATGCGCC TGGCATTGGA TGGCGGCGAT CCTACGACGA CGGCGACATC 
ACACCCACCA ACGATGCCAC GAGGGAGCTG CTCTACGAGC AGGAGGGCGG CGGAACTCTG 
CCACGCCAGC AGCGTGGCAT CCTGCAGCGG GCGGTGTTGA ACACCATGCC GCCACTGGAG 
CACCATCACT ACATGAATGC AGCGGCTGCG GCGGAGTACG GTGCGGCCAG CGGTGTGGAG 
GGTGGAGCGA ATTACATGGG CGGCAGTCCT TTGACGGGCA AGAAAATAGC TCCTGAGCCA 
CCTAGACGCC ACTGCAGCAT ACGCAACTCC CGCACGCTGA GCGGAGAGGG AGTGCCGTCG 
GGAGCGGCTG CGCAGGCGCA TCAGCAGGCA GCCCAACAGC AGCAGGGCCA GCACATGTTC 
TACGGCCACA CGGCGCAGCA CTGGCAGCAG CAGCAGCAGC AGGCGGGTGC AGGTACTGGT 
CAGCAGCCGA TCTACGCCAA CTATGCCACC ATACAACAGA CAACGGCGGA GATACACTGT 
GAGAAGTATC ACGACAGTAA GTCGACTTCC AGCATCGATT CGATTGACAC GATACCATTT 
GCCAACGAGA ACACTGGGAC GATAAAACAG CGGCTGCTCA ACCGCCAGGA GCTGCAGGGC 
GGCGGAGCGG GCAACAACAA TGTACCCGGC GGCGGCCATC CCGCACACCT GCACTCCTCC 
AGCTCGAGCT CCAGCTCCTC CTCCACGAAC ACCATCGCGA ATATAGCGCA CTCGTTCCAT 
TCGTTGAAAT CTTCCAGTTC GCTGCACGAT GCCACATTGG CGCGGAGTGA AAGCATGGGC 
AGCACAGCCA GCGGTGGAAG TGCCTTGCAC CTGCGTTCAC CCACATTGGA TATGTCAAAT 
GCCGGTGGAT CAACGGCGGA GCCTGCAAGT TCGCCAGAAG CAGCCGTACC AACTGTGGCC 
ATAAACAACG GCGTTCCGCC CAAAGTTTCG GTGAACGTTC TGAACGACAT TGGCAATATG 
CTGGCCAATC TCACGGACGA GCTGGACGCC ATGCTGGAGG AGGAGAAGCG AGTGGGCCTA 
AACATAGACA GTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckn ( NM_001299509.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286438.1 Drosophila melanogaster caskin (ckn), transcript variant F, mRNA.

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CloneID	OFa21813
Clone ID Related Accession (Same CDS sequence)	NM_001299509.1
Accession Version	NM_001299509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	caskin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCGT GCCTGCTGGA GATCTTCGCC CATTTGTTCG AGGATGGAGG ATTTCGATTA 
GCATATGCGG CTTATAGAGT TGCGGTCTTG TTATTATTGT TGTTTTGCTG CGACATTCAC 
TTCGACGGAG TTGGATCCAT AGAGGAATGG CTGCAGCTGC TGCGTCTGGA GGAGTATATA 
CAGCCCCTGC TGGAGCAGAA CTATAAGACG GTGCGCGACG TAACCCAAGT GACTTGGGAG 
GATCTCGAGG ACATTGGCAT TGTCAAGCTG GGCCACCAAA AGAAAATTCT GCTGGCCATC 
AAGCGTGTGA AGGACATAAT TAGCGGAAAG TGGAACCCAG GCGGCGCCAA TGCCTACCAA 
CAGCAGGAGT ACCAGCGGAA GCCCTGGCAA TTCAATGTGC CCATACCCAG TACGCCATCC 
TATGTGCCAA CCACCCGCGT CTCGTGGGAC GACACGAAAA TCTACGCGAC CAGCAGTGTC 
TTGAATTCCA CTGCCCCATC CGGGAGCAGC TGCCTCAATA GCAGCCTTGC CAACGGAGAT 
GCCTACTACT GCACGGGCAG TAACCATGAG TGCTGCTCGG GCAACGATGT GGTGCTCATT 
AAGGTTCGCC AGCCGCGCGG CAAGAGCCTG GAATCTTTGG AGGACATCCA CGACAACAGC 
ACGCGCAGCC ATCTGACCTT CAGCCATTAC ACAACCACGG ACTACGGCCA CTTTGGGCCG 
CCGACAACAG CGGCGGCTGC AGCGGCAATG GCCGCAGCGG CCACACTGCA GCAGCAGCAT 
CATCACCAGC AGCTGCTCCA CCAGCAACAG CAGCAGGCAG CTGCCGCCCG TCTCCTGGGC 
AACAATGCGC CTGGCATTGG ATGGCGGCGA TCCTACGACG ACGGCGACAT CACACCCACC 
AACGATGCCA CGAGGGAGCT GCTCTACGAG CAGGAGGGCG GCGGAACTCT GCCACGCCAG 
CAGCGTGGCA TCCTGCAGCG GGCGGTGTTG AACACCATGC CGCCACTGGA GCACCATCAC 
TACATGAATG CAGCGGCTGC GGCGGAGTAC GGTGCGGCCA GCGGTGTGGA GGGTGGAGCG 
AATTACATGG GCGGCAGTCC TTTGACGGGC AAGAAAATAG CTCCTGAGCC ACCTAGACGC 
CACTGCAGCA TACGCAACTC CCGCACGCTG AGCGGAGAGG GAGTGCCGTC GGGAGCGGCT 
GCGCAGGCGC ATCAGCAGGC AGCCCAACAG CAGCAGGGCC AGCACATGTT CTACGGCCAC 
ACGGCGCAGC ACTGGCAGCA GCAGCAGCAG CAGGCGGGTG CAGGTACTGG TCAGCAGCCG 
ATCTACGCCA ACTATGCCAC CATACAACAG ACAACGGCGG AGATACACTG TGAGAAGTAT 
CACGACAGTA AGTCGACTTC CAGCATCGAT TCGATTGACA CGATACCATT TGCCAACGAG 
AACACTGGGA CGATAAAACA GCGGCTGCTC AACCGCCAGG AGCTGCAGGG CGGCGGAGCG 
GGCAACAACA ATGTACCCGG CGGCGGCCAT CCCGCACACC TGCACTCCTC CAGCTCGAGC 
TCCAGCTCCT CCTCCACGAA CACCATCGCG AATATAGCGC ACTCGTTCCA TTCGTTGAAA 
TCTTCCAGTT CGCTGCACGA TGCCACATTG GCGCGGAGTG AAAGCATGGG CAGCACAGCC 
AGCGGTGGAA GTGCCTTGCA CCTGCGTTCA CCCACATTGG ATATGTCAAA TGCCGGTGGA 
TCAACGGCGG AGCCTGCAAG TTCGCCAGAA GCAGCCGTAC CAACTGTGGC CATAAACAAC 
GGCGTTCCGC CCAAAGTTTC GGTGAACGTT CTGAACGACA TTGGCAATAT GCTGGCCAAT 
CTCACGGACG AGCTGGACGC CATGCTGGAG GAGGAGAAGC GAGTGGGCCT AAACATAGAC 
AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286438.1
CDS	127..2055
Translation	MVSCLLEIFAHLFEDGGFRLAYAAYRVAVLLLLLFCCDIHFDGVGSIEEWLQLLRLEEYIQPLLEQNYKTVRDVTQVTWEDLEDIGIVKLGHQKKILLAIKRVKDIISGKWNPGGANAYQQQEYQRKPWQFNVPIPSTPSYVPTTRVSWDDTKIYATSSVLNSTAPSGSSCLNSSLANGDAYYCTGSNHECCSGNDVVLIKVRQPRGKSLESLEDIHDNSTRSHLTFSHYTTTDYGHFGPPTTAAAAAAMAAAATLQQQHHHQQLLHQQQQQAAAARLLGNNAPGIGWRRSYDDGDITPTNDATRELLYEQEGGGTLPRQQRGILQRAVLNTMPPLEHHHYMNAAAAAEYGAASGVEGGANYMGGSPLTGKKIAPEPPRRHCSIRNSRTLSGEGVPSGAAAQAHQQAAQQQQGQHMFYGHTAQHWQQQQQQAGAGTGQQPIYANYATIQQTTAEIHCEKYHDSKSTSSIDSIDTIPFANENTGTIKQRLLNRQELQGGGAGNNNVPGGGHPAHLHSSSSSSSSSSTNTIANIAHSFHSLKSSSSLHDATLARSESMGSTASGGSALHLRSPTLDMSNAGGSTAEPASSPEAAVPTVAINNGVPPKVSVNVLNDIGNMLANLTDELDAMLEEEKRVGLNIDSE

Target ORF information:

RefSeq Version	NM_001299509.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster caskin (ckn), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299509.1

ORF Insert Sequence:

ATGGTGTCGT GCCTGCTGGA GATCTTCGCC CATTTGTTCG AGGATGGAGG ATTTCGATTA 
GCATATGCGG CTTATAGAGT TGCGGTCTTG TTATTATTGT TGTTTTGCTG CGACATTCAC 
TTCGACGGAG TTGGATCCAT AGAGGAATGG CTGCAGCTGC TGCGTCTGGA GGAGTATATA 
CAGCCCCTGC TGGAGCAGAA CTATAAGACG GTGCGCGACG TAACCCAAGT GACTTGGGAG 
GATCTCGAGG ACATTGGCAT TGTCAAGCTG GGCCACCAAA AGAAAATTCT GCTGGCCATC 
AAGCGTGTGA AGGACATAAT TAGCGGAAAG TGGAACCCAG GCGGCGCCAA TGCCTACCAA 
CAGCAGGAGT ACCAGCGGAA GCCCTGGCAA TTCAATGTGC CCATACCCAG TACGCCATCC 
TATGTGCCAA CCACCCGCGT CTCGTGGGAC GACACGAAAA TCTACGCGAC CAGCAGTGTC 
TTGAATTCCA CTGCCCCATC CGGGAGCAGC TGCCTCAATA GCAGCCTTGC CAACGGAGAT 
GCCTACTACT GCACGGGCAG TAACCATGAG TGCTGCTCGG GCAACGATGT GGTGCTCATT 
AAGGTTCGCC AGCCGCGCGG CAAGAGCCTG GAATCTTTGG AGGACATCCA CGACAACAGC 
ACGCGCAGCC ATCTGACCTT CAGCCATTAC ACAACCACGG ACTACGGCCA CTTTGGGCCG 
CCGACAACAG CGGCGGCTGC AGCGGCAATG GCCGCAGCGG CCACACTGCA GCAGCAGCAT 
CATCACCAGC AGCTGCTCCA CCAGCAACAG CAGCAGGCAG CTGCCGCCCG TCTCCTGGGC 
AACAATGCGC CTGGCATTGG ATGGCGGCGA TCCTACGACG ACGGCGACAT CACACCCACC 
AACGATGCCA CGAGGGAGCT GCTCTACGAG CAGGAGGGCG GCGGAACTCT GCCACGCCAG 
CAGCGTGGCA TCCTGCAGCG GGCGGTGTTG AACACCATGC CGCCACTGGA GCACCATCAC 
TACATGAATG CAGCGGCTGC GGCGGAGTAC GGTGCGGCCA GCGGTGTGGA GGGTGGAGCG 
AATTACATGG GCGGCAGTCC TTTGACGGGC AAGAAAATAG CTCCTGAGCC ACCTAGACGC 
CACTGCAGCA TACGCAACTC CCGCACGCTG AGCGGAGAGG GAGTGCCGTC GGGAGCGGCT 
GCGCAGGCGC ATCAGCAGGC AGCCCAACAG CAGCAGGGCC AGCACATGTT CTACGGCCAC 
ACGGCGCAGC ACTGGCAGCA GCAGCAGCAG CAGGCGGGTG CAGGTACTGG TCAGCAGCCG 
ATCTACGCCA ACTATGCCAC CATACAACAG ACAACGGCGG AGATACACTG TGAGAAGTAT 
CACGACAGTA AGTCGACTTC CAGCATCGAT TCGATTGACA CGATACCATT TGCCAACGAG 
AACACTGGGA CGATAAAACA GCGGCTGCTC AACCGCCAGG AGCTGCAGGG CGGCGGAGCG 
GGCAACAACA ATGTACCCGG CGGCGGCCAT CCCGCACACC TGCACTCCTC CAGCTCGAGC 
TCCAGCTCCT CCTCCACGAA CACCATCGCG AATATAGCGC ACTCGTTCCA TTCGTTGAAA 
TCTTCCAGTT CGCTGCACGA TGCCACATTG GCGCGGAGTG AAAGCATGGG CAGCACAGCC 
AGCGGTGGAA GTGCCTTGCA CCTGCGTTCA CCCACATTGG ATATGTCAAA TGCCGGTGGA 
TCAACGGCGG AGCCTGCAAG TTCGCCAGAA GCAGCCGTAC CAACTGTGGC CATAAACAAC 
GGCGTTCCGC CCAAAGTTTC GGTGAACGTT CTGAACGACA TTGGCAATAT GCTGGCCAAT 
CTCACGGACG AGCTGGACGC CATGCTGGAG GAGGAGAAGC GAGTGGGCCT AAACATAGAC 
AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ckn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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