Pcf11 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcf11
Entrez Gene ID	36658
Full Name	Protein 1 of cleavage and polyadenylation factor 1
Synonyms	CG10228,Dmel\CG10228,Inr-a,anon-WO0118547.146,dPcf11,l(2)k08015,pcf11
General protein information	Preferred Names Protein 1 of cleavage and polyadenylation factor 1 Names CG10228 gene product from transcript CG10228-RH CG10228-PB CG10228-PC CG10228-PD CG10228-PE CG10228-PF CG10228-PG CG10228-PH Pcf11-PB Pcf11-PC Pcf11-PD Pcf11-PE Pcf11-PF Pcf11-PG Pcf11-PH inverse regulator a lethal (2) k08015 protein 1 of cleavage and polyadenylation factor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51C5-50B9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299499.1	NP_001286428.1	protein 1 of cleavage and polyadenylation factor 1, isoform F
NM_001202002.2	NP_001188931.1	protein 1 of cleavage and polyadenylation factor 1, isoform E
NM_001299501.1	NP_001286430.1	protein 1 of cleavage and polyadenylation factor 1, isoform H
NM_001169683.2	NP_001163154.1	protein 1 of cleavage and polyadenylation factor 1, isoform C
NM_001299500.1	NP_001286429.1	protein 1 of cleavage and polyadenylation factor 1, isoform G
NM_001169682.2	NP_001163153.1	protein 1 of cleavage and polyadenylation factor 1, isoform B
NM_137155.5	NP_610999.4	protein 1 of cleavage and polyadenylation factor 1, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Pcf11 is directly involved in transcription termination in Drosophila.
Title: Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript.

The following Pcf11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcf11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12553	NM_001169682.2 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant B (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12554	NM_001169683.2 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant C (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10032	NM_137155.5 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant D (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12552	NM_001299501.1 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant H (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12552	NM_001202002.2 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant E (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21809	NM_001299499.1 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant F (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21810	NM_001299500.1 Latest version!	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant G (Pcf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcf11 ( NM_001169682.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163153.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant B (Pcf11), mRNA.

Price & Availability↑

CloneID	OFa12553
Clone ID Related Accession (Same CDS sequence)	NM_001169682.2
Accession Version	NM_001169682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169682.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGTAAGAA ACAACTTTTA 
ATTCTAATCT CACACTTGTT AGCGAACAAC CAAGTCTGGT TTGACTTGGA ACCTAGAGCT 
ACAGACAGCT TAGTTGAAAA TTTAGAATAT ATCAACCAGA ATGGCGGCAC TCGATGGCTT 
AGGTTGGTGG AAGGGCGGGT TATTGGGACC GTATGTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163153.1
CDS	234..1955
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKRKKQLLILISHLLANNQVWFDLEPRATDSLVENLEYINQNGGTRWLRLVEGRVIGTVCG

Target ORF information:

RefSeq Version	NM_001169682.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant B (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169682.2

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGTAAGAA ACAACTTTTA 
ATTCTAATCT CACACTTGTT AGCGAACAAC CAAGTCTGGT TTGACTTGGA ACCTAGAGCT 
ACAGACAGCT TAGTTGAAAA TTTAGAATAT ATCAACCAGA ATGGCGGCAC TCGATGGCTT 
AGGTTGGTGG AAGGGCGGGT TATTGGGACC GTATGTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_001169683.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163154.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant C (Pcf11), mRNA.

Price & Availability↑

CloneID	OFa12554
Clone ID Related Accession (Same CDS sequence)	NM_001169683.2
Accession Version	NM_001169683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169683.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTTAACA AAACAAAAGC GTATTTACTT AAAAACAAAG ACACTCCAGC TTACACACAG 
CTAATAGACG GTCGACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163154.1
CDS	234..494
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVNKTKAYLLKNKDTPAYTQLIDGRH

Target ORF information:

RefSeq Version	NM_001169683.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant C (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169683.2

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTTAACA AAACAAAAGC GTATTTACTT AAAAACAAAG ACACTCCAGC TTACACACAG 
CTAATAGACG GTCGACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_137155.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610999.4 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant D (Pcf11), mRNA.

Price & Availability↑

CloneID	OFa10032
Clone ID Related Accession (Same CDS sequence)	NM_137155.5
Accession Version	NM_137155.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137155.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACCTACATT TCAAATACTC 
GAACCAGGCC ATCCTCCAGC TCCTCCAAGT CCGCCGATTA TAGATGTATC GAGTATGGAC 
ATTGACTTGA GGCGGCCAAA GACGCCGCCT CCTCCGACAT TCGACTCAGT GGATAGCCTC 
GACCAGAAGG ATACAACTAG CGGTCACATC AGCAGCACAA TAATACAAGC TAGTGGCGCC 
TCCAACGCCT CCCCATCAGG CGCGTCCACT TCCAAATTGC CCGCAAAAGT GTCGTTCAAA 
ATACAAAAAC AGTTCAATAA ATTAGAAAAA TCTACAGCGA ATCTATCAAC AGTATCAATG 
CTCAACTCAA ATCCCACGAC TTCAGGAACA CCAGCGGCAT CTCTAGTCAG GCAATCGTCG 
CCCAGTCCTT CGTCCTCGTC TATTTCCTCG CAGGGTAATG TATCGGACGC CCTGCAGCTG 
TCGATTAACA AGTTGCTCCA GGTGCAAGGC GGTGCCGGCG AGAAACGTCC CGCCGACGCA 
CTGCCGCCAC ATATCGACGG TGAAGAGCAA CCGCCGCAGC AAAAGCGCAG CAAGTCAGCC 
AAACTGGACG CTCTCTTTGG CAGTGAGGAT GTCGACCTGC GACGCGAGAT TCTAGTTGTC 
AAGCCAGGAG TTATTGTTGT CGAAGACGAC TCTATGGACG AATGCGATGT AGACAAGCCC 
ACAAAGAAAT CTGGGTCGCC GCCCAAGAAG GCAACGTTGG AGGAGCTGCG CGCCAAGTTG 
GCTAACTCGG CTCGAATCCA GAATAAGCAG GGAAAGTCTG ACAAATCCAG GGATCAGGCC 
GTGTCACAAC GCCTTAAGCA GCTGGCGGAG CTTAAAGTCA ACGACGATTC GCAGGAGGCG 
CACGACGAGA AGGTGCGCAC CATTCTCAGC CAGGCGCAGG AGATGTATGA GAATAACGGC 
ATGAATCAGG AACAGTACAA GGATCTGGTC CAAAAAGTAG TGGCCATCAA TGAGAATAGC 
AAAATAAAAG AGTCCCGGCG CCGAGACAAC GATCTCGAGC GCAATGCGGC CAGGGATGCA 
GTGCTACGCA AACGTATTCC CAAGCTGAAG GGCAACGAAA ATCATTCTGC GGGCTCACCG 
CATACCGATG GATCGCCCAG GTACGATGAT CAGCCGGCAG CTGCCCCGGA GAAGCCCTCA 
AACAAACGGG ATAAGACCAA GAGGGAGATG AAGCGTCGCA AGCCCACCAA ATGGGGCGAA 
CAAGTGGATG CAGGGGCAGC ACAACGCACT GCCTGGCAAC TGGCCAATGT CAACAATAAT 
AATAATAATA ACAACAACAA GCGGGGCGGT ATCCAATTGC CCGTTCAGCC TCAGGCTGGA 
TTCCGTGGCA TGCCCTGGCA GCAGCCACCA GCCATGGTGT TGGGCCAGTC CGCAGTTCCG 
CCGCCACCGC AACCGCCGTC GATGATTAGT ATGCCTCCCG TGCCACCGGT GACCATGACC 
AAGGCCATAA ATTCCCTTGA CAATCCAATG GCTGATGTAG TGCGAAGCAT CACGATCGAC 
GGTGGCTCCA AGGAGATACG CTTCTACAAC CAGGTGGCCA TCATTTTCAT GGATGGCGAT 
CAGCCGCATG AAATTGGCTT CCAGCCGGGT CAGCGGGTGA TCTTCATTGA TCACAATGAA 
CCGCTACCGC TTTGCTTCAA CGACGATTAC AAGCCTTTCC ACTTGGATGG ACAGCTTCAC 
CGGATTCGGT TTGGCTTTCC ATCGCGCGAA CTTTATATCG ACGAGCACTG GTACGAGATC 
TACTTTGGCG GTCCAGCCGT ATCCGTGCCC ATTGGCAACA AACTGCATAT AATCAAAGCT 
GAGGGTCCAC CGCCCAACGT GGACATTGGT CGAGTGCGAA GGGATCTGGT TGTGGGCAAA 
ATCAATATGA TTGCGGATGC GCATACTATA GTGCCACTCT TTCTGGACGC CAGACAGCAG 
ACCTTCCAGC TCGGCGCCGA GCAGCATTCA CTGCAGTTCG TGGACAGTTT CCAATATGCT 
CTGCTCGACG GCCAGCTGCA GAAGATGGAG TATGGTGGTC TCCCAAAAGG AATGATGCTG 
AACGGCGGTC GCAGCTGCTT CATTAGGTTT GGAACGCTGC CCAAAGGAGT CATTGCTGGA 
AAAACCCATG TGGCCGATAT GGTGTACATT AAGACTGATG CTCCTGCAGA GCCTCCCAAG 
CCGCCGCCAA TTATTGTGAA ACCACCGCCA GTGGTGGAGC AACCGAAGCC CGCTCCAGTT 
GCTGTTGCTC CCATTTCCCT TCCAGCAGCA GCCGCCGCAC TTGAGAGCTT AAACATCAAT 
GATTTGTTTC AAAAGCTTGT CTCGTCTGGA ATAATTGGTG GTGCAACTGC AGCACCAACT 
CTCCCTGCCG CAGACTCATC AGCGGCCAAA GAGCCAACCG CTTCTGCCAC CGAACCCTCA 
ACTGCATCTG CAACCGTTGC GCCTGCTACA CTGCCTGCTG GTCCCCCCGC GGAGCCGATC 
AAGCGCATCG ATCTCCGCAA GCCAGAGTCC ATCAAGACAC GCCAGGCGGC TGTGGTGGCC 
GTTCTTTACC TGGGAATGCA GTGCAGCAGC TGTGGAGTTC GTTTCCCGCC AGAGCAAACA 
ATTAAGTACA GCCAACATTT GGACTGGCAC TTCCGACAGA ATCGTAGAGA GCGGGATTCC 
ACTAGGAAAG CCACTTCGCG AAAATGGTAT TACGATCTCA ACGACTGGCG GCAATACGAA 
GAAATCGAAG ATGTGGAGGA GCGAGAGAAG AACTTCCTCG AGGCTCAGGG ACAGCCAGGT 
GGCGTTGAGG CCCTTGATGA ACTCTCACAA CAACGCTCGC TGGATTCGCC CGTGCCAACA 
TGTGCTGCTG GAAGGGATGA TGTGGATCAC TGCTGTGACA TGTGTCACGA GAAATTCGAG 
CAGTTCTACA ACGAAGAGCT CGAGGAGTGG CATCTGCGTA GCGCTATTCG TGTGGAAGAT 
AAGATCTATC ATCCTCTATG CTACGAGGAC TACAAGGCCT CGTTGAATCC TCCAACGGAG 
GTAAAGTCCG ACCAGGATGT GGACATGAAC AACACCGATG ACAATGCCAT GGACACACTA 
ATCAAAGTGG AGGATGATGA AGATGAAGGT ACGCCAAACT CATCACAACC CATTTTTGAA 
GACGACGATG ACGATGTCAT TGTGCTGCCC AATGAAGAGC CAAGTGTCAC GGAAATCGTC 
GACGACGACG ATGAGGACGA ATACGTTCCG GGCAATGTGA CACGTGCGGA CATGGGCAAC 
GAGTCACAGG ACAAAACAGA GTCCAACTCC GAGACCAAAG AACCGAAGAA AGATCAGGTT 
GAGCTCGGTG AACAGCAGCA AAACGAATCG GCCAATGAAT CGGATGTCGA AATCCAAGAG 
CCGAACATTC CTTTCACGGA TCTGGACACT TATGTGGAGA AAGAGATGGA TGAGGCGACC 
AGGGCGGCGC TACTGAACGT AAAGATCAAG GAGGAGCCCA AGGATGAGTA TGAGGAGGAC 
GAGGATGATG GATTCGAAGA CGTGGGCACG GTGGTTTCAC TTTTACCTCT GCCCGAGGAC 
GAGATCTCCA TACACAGCAG CGAAACTCAA ACACACACCA TCGGCTCGTC AGCCTCGCCG 
GCCACCATCG AGCGGCCAGC ATCGGTGACC TCGCTCTCCC TGCCCGCCAA TGAGGCGGAC 
GAGTTGGAGC AGGGTGATGC AAACACCGAT ACGGATGCGG AATTGAATGG CGAGAAGCAG 
GAGGCCACGC ATAACCTGAG CGCAGTGGGT CCGGCGTTAC CTTTGGCTAG CATAGTTAAT 
AAAATAAAGA TAAATATCAC TAAGAATACG AGTAGTAATA GTCATAATAG TGCCTCCAAC 
ACCACGACAA CGGACTCGCA AGTCTCGGCC ATAAGCGTCA TCGGTGGATC AGGAGCAGCT 
GGCGGTGCAT CCGGAGCAGG AGACCCTGTC CAGCAGGTGA ATGCCATTCA AACCATTTCC 
ACCATTCCAG TGCTGTGCGG CGGCAATACG TTCGTGCCCA AGATTGCGAC CAGCACGCCC 
TCAAACGTAA TCAGCTCGAT CTCGGTAATT GGCAGCACTT ACGGCGCAAG CAGCAGCAGC 
AATGGCAGCC GAAGCACTAC TTCCACCGCA TCCTCCGCAC CAGCTGCTTC GCTTTTGGCG 
GAGACGGGCA GCCTGAAATC GCCAACTCCG CCGCTCGCCA CAGTTGATCC GGATCCGGAG 
CCCGTCGTGG AACAAAAGCC GGCGCTGCGG AATGCCACGC TGAAGCGGAC GAAAAAGGTG 
CAGAACGGCA TCGAAACGTC GGGCCTGTGC TCGATCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610999.4
CDS	234..6095
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKPTFQILEPGHPPAPPSPPIIDVSSMDIDLRRPKTPPPPTFDSVDSLDQKDTTSGHISSTIIQASGASNASPSGASTSKLPAKVSFKIQKQFNKLEKSTANLSTVSMLNSNPTTSGTPAASLVRQSSPSPSSSSISSQGNVSDALQLSINKLLQVQGGAGEKRPADALPPHIDGEEQPPQQKRSKSAKLDALFGSEDVDLRREILVVKPGVIVVEDDSMDECDVDKPTKKSGSPPKKATLEELRAKLANSARIQNKQGKSDKSRDQAVSQRLKQLAELKVNDDSQEAHDEKVRTILSQAQEMYENNGMNQEQYKDLVQKVVAINENSKIKESRRRDNDLERNAARDAVLRKRIPKLKGNENHSAGSPHTDGSPRYDDQPAAAPEKPSNKRDKTKREMKRRKPTKWGEQVDAGAAQRTAWQLANVNNNNNNNNNKRGGIQLPVQPQAGFRGMPWQQPPAMVLGQSAVPPPPQPPSMISMPPVPPVTMTKAINSLDNPMADVVRSITIDGGSKEIRFYNQVAIIFMDGDQPHEIGFQPGQRVIFIDHNEPLPLCFNDDYKPFHLDGQLHRIRFGFPSRELYIDEHWYEIYFGGPAVSVPIGNKLHIIKAEGPPPNVDIGRVRRDLVVGKINMIADAHTIVPLFLDARQQTFQLGAEQHSLQFVDSFQYALLDGQLQKMEYGGLPKGMMLNGGRSCFIRFGTLPKGVIAGKTHVADMVYIKTDAPAEPPKPPPIIVKPPPVVEQPKPAPVAVAPISLPAAAAALESLNINDLFQKLVSSGIIGGATAAPTLPAADSSAAKEPTASATEPSTASATVAPATLPAGPPAEPIKRIDLRKPESIKTRQAAVVAVLYLGMQCSSCGVRFPPEQTIKYSQHLDWHFRQNRRERDSTRKATSRKWYYDLNDWRQYEEIEDVEEREKNFLEAQGQPGGVEALDELSQQRSLDSPVPTCAAGRDDVDHCCDMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCYEDYKASLNPPTEVKSDQDVDMNNTDDNAMDTLIKVEDDEDEGTPNSSQPIFEDDDDDVIVLPNEEPSVTEIVDDDDEDEYVPGNVTRADMGNESQDKTESNSETKEPKKDQVELGEQQQNESANESDVEIQEPNIPFTDLDTYVEKEMDEATRAALLNVKIKEEPKDEYEEDEDDGFEDVGTVVSLLPLPEDEISIHSSETQTHTIGSSASPATIERPASVTSLSLPANEADELEQGDANTDTDAELNGEKQEATHNLSAVGPALPLASIVNKIKINITKNTSSNSHNSASNTTTTDSQVSAISVIGGSGAAGGASGAGDPVQQVNAIQTISTIPVLCGGNTFVPKIATSTPSNVISSISVIGSTYGASSSSNGSRSTTSTASSAPAASLLAETGSLKSPTPPLATVDPDPEPVVEQKPALRNATLKRTKKVQNGIETSGLCSIM

Target ORF information:

RefSeq Version	NM_137155.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant D (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137155.5

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACCTACATT TCAAATACTC 
GAACCAGGCC ATCCTCCAGC TCCTCCAAGT CCGCCGATTA TAGATGTATC GAGTATGGAC 
ATTGACTTGA GGCGGCCAAA GACGCCGCCT CCTCCGACAT TCGACTCAGT GGATAGCCTC 
GACCAGAAGG ATACAACTAG CGGTCACATC AGCAGCACAA TAATACAAGC TAGTGGCGCC 
TCCAACGCCT CCCCATCAGG CGCGTCCACT TCCAAATTGC CCGCAAAAGT GTCGTTCAAA 
ATACAAAAAC AGTTCAATAA ATTAGAAAAA TCTACAGCGA ATCTATCAAC AGTATCAATG 
CTCAACTCAA ATCCCACGAC TTCAGGAACA CCAGCGGCAT CTCTAGTCAG GCAATCGTCG 
CCCAGTCCTT CGTCCTCGTC TATTTCCTCG CAGGGTAATG TATCGGACGC CCTGCAGCTG 
TCGATTAACA AGTTGCTCCA GGTGCAAGGC GGTGCCGGCG AGAAACGTCC CGCCGACGCA 
CTGCCGCCAC ATATCGACGG TGAAGAGCAA CCGCCGCAGC AAAAGCGCAG CAAGTCAGCC 
AAACTGGACG CTCTCTTTGG CAGTGAGGAT GTCGACCTGC GACGCGAGAT TCTAGTTGTC 
AAGCCAGGAG TTATTGTTGT CGAAGACGAC TCTATGGACG AATGCGATGT AGACAAGCCC 
ACAAAGAAAT CTGGGTCGCC GCCCAAGAAG GCAACGTTGG AGGAGCTGCG CGCCAAGTTG 
GCTAACTCGG CTCGAATCCA GAATAAGCAG GGAAAGTCTG ACAAATCCAG GGATCAGGCC 
GTGTCACAAC GCCTTAAGCA GCTGGCGGAG CTTAAAGTCA ACGACGATTC GCAGGAGGCG 
CACGACGAGA AGGTGCGCAC CATTCTCAGC CAGGCGCAGG AGATGTATGA GAATAACGGC 
ATGAATCAGG AACAGTACAA GGATCTGGTC CAAAAAGTAG TGGCCATCAA TGAGAATAGC 
AAAATAAAAG AGTCCCGGCG CCGAGACAAC GATCTCGAGC GCAATGCGGC CAGGGATGCA 
GTGCTACGCA AACGTATTCC CAAGCTGAAG GGCAACGAAA ATCATTCTGC GGGCTCACCG 
CATACCGATG GATCGCCCAG GTACGATGAT CAGCCGGCAG CTGCCCCGGA GAAGCCCTCA 
AACAAACGGG ATAAGACCAA GAGGGAGATG AAGCGTCGCA AGCCCACCAA ATGGGGCGAA 
CAAGTGGATG CAGGGGCAGC ACAACGCACT GCCTGGCAAC TGGCCAATGT CAACAATAAT 
AATAATAATA ACAACAACAA GCGGGGCGGT ATCCAATTGC CCGTTCAGCC TCAGGCTGGA 
TTCCGTGGCA TGCCCTGGCA GCAGCCACCA GCCATGGTGT TGGGCCAGTC CGCAGTTCCG 
CCGCCACCGC AACCGCCGTC GATGATTAGT ATGCCTCCCG TGCCACCGGT GACCATGACC 
AAGGCCATAA ATTCCCTTGA CAATCCAATG GCTGATGTAG TGCGAAGCAT CACGATCGAC 
GGTGGCTCCA AGGAGATACG CTTCTACAAC CAGGTGGCCA TCATTTTCAT GGATGGCGAT 
CAGCCGCATG AAATTGGCTT CCAGCCGGGT CAGCGGGTGA TCTTCATTGA TCACAATGAA 
CCGCTACCGC TTTGCTTCAA CGACGATTAC AAGCCTTTCC ACTTGGATGG ACAGCTTCAC 
CGGATTCGGT TTGGCTTTCC ATCGCGCGAA CTTTATATCG ACGAGCACTG GTACGAGATC 
TACTTTGGCG GTCCAGCCGT ATCCGTGCCC ATTGGCAACA AACTGCATAT AATCAAAGCT 
GAGGGTCCAC CGCCCAACGT GGACATTGGT CGAGTGCGAA GGGATCTGGT TGTGGGCAAA 
ATCAATATGA TTGCGGATGC GCATACTATA GTGCCACTCT TTCTGGACGC CAGACAGCAG 
ACCTTCCAGC TCGGCGCCGA GCAGCATTCA CTGCAGTTCG TGGACAGTTT CCAATATGCT 
CTGCTCGACG GCCAGCTGCA GAAGATGGAG TATGGTGGTC TCCCAAAAGG AATGATGCTG 
AACGGCGGTC GCAGCTGCTT CATTAGGTTT GGAACGCTGC CCAAAGGAGT CATTGCTGGA 
AAAACCCATG TGGCCGATAT GGTGTACATT AAGACTGATG CTCCTGCAGA GCCTCCCAAG 
CCGCCGCCAA TTATTGTGAA ACCACCGCCA GTGGTGGAGC AACCGAAGCC CGCTCCAGTT 
GCTGTTGCTC CCATTTCCCT TCCAGCAGCA GCCGCCGCAC TTGAGAGCTT AAACATCAAT 
GATTTGTTTC AAAAGCTTGT CTCGTCTGGA ATAATTGGTG GTGCAACTGC AGCACCAACT 
CTCCCTGCCG CAGACTCATC AGCGGCCAAA GAGCCAACCG CTTCTGCCAC CGAACCCTCA 
ACTGCATCTG CAACCGTTGC GCCTGCTACA CTGCCTGCTG GTCCCCCCGC GGAGCCGATC 
AAGCGCATCG ATCTCCGCAA GCCAGAGTCC ATCAAGACAC GCCAGGCGGC TGTGGTGGCC 
GTTCTTTACC TGGGAATGCA GTGCAGCAGC TGTGGAGTTC GTTTCCCGCC AGAGCAAACA 
ATTAAGTACA GCCAACATTT GGACTGGCAC TTCCGACAGA ATCGTAGAGA GCGGGATTCC 
ACTAGGAAAG CCACTTCGCG AAAATGGTAT TACGATCTCA ACGACTGGCG GCAATACGAA 
GAAATCGAAG ATGTGGAGGA GCGAGAGAAG AACTTCCTCG AGGCTCAGGG ACAGCCAGGT 
GGCGTTGAGG CCCTTGATGA ACTCTCACAA CAACGCTCGC TGGATTCGCC CGTGCCAACA 
TGTGCTGCTG GAAGGGATGA TGTGGATCAC TGCTGTGACA TGTGTCACGA GAAATTCGAG 
CAGTTCTACA ACGAAGAGCT CGAGGAGTGG CATCTGCGTA GCGCTATTCG TGTGGAAGAT 
AAGATCTATC ATCCTCTATG CTACGAGGAC TACAAGGCCT CGTTGAATCC TCCAACGGAG 
GTAAAGTCCG ACCAGGATGT GGACATGAAC AACACCGATG ACAATGCCAT GGACACACTA 
ATCAAAGTGG AGGATGATGA AGATGAAGGT ACGCCAAACT CATCACAACC CATTTTTGAA 
GACGACGATG ACGATGTCAT TGTGCTGCCC AATGAAGAGC CAAGTGTCAC GGAAATCGTC 
GACGACGACG ATGAGGACGA ATACGTTCCG GGCAATGTGA CACGTGCGGA CATGGGCAAC 
GAGTCACAGG ACAAAACAGA GTCCAACTCC GAGACCAAAG AACCGAAGAA AGATCAGGTT 
GAGCTCGGTG AACAGCAGCA AAACGAATCG GCCAATGAAT CGGATGTCGA AATCCAAGAG 
CCGAACATTC CTTTCACGGA TCTGGACACT TATGTGGAGA AAGAGATGGA TGAGGCGACC 
AGGGCGGCGC TACTGAACGT AAAGATCAAG GAGGAGCCCA AGGATGAGTA TGAGGAGGAC 
GAGGATGATG GATTCGAAGA CGTGGGCACG GTGGTTTCAC TTTTACCTCT GCCCGAGGAC 
GAGATCTCCA TACACAGCAG CGAAACTCAA ACACACACCA TCGGCTCGTC AGCCTCGCCG 
GCCACCATCG AGCGGCCAGC ATCGGTGACC TCGCTCTCCC TGCCCGCCAA TGAGGCGGAC 
GAGTTGGAGC AGGGTGATGC AAACACCGAT ACGGATGCGG AATTGAATGG CGAGAAGCAG 
GAGGCCACGC ATAACCTGAG CGCAGTGGGT CCGGCGTTAC CTTTGGCTAG CATAGTTAAT 
AAAATAAAGA TAAATATCAC TAAGAATACG AGTAGTAATA GTCATAATAG TGCCTCCAAC 
ACCACGACAA CGGACTCGCA AGTCTCGGCC ATAAGCGTCA TCGGTGGATC AGGAGCAGCT 
GGCGGTGCAT CCGGAGCAGG AGACCCTGTC CAGCAGGTGA ATGCCATTCA AACCATTTCC 
ACCATTCCAG TGCTGTGCGG CGGCAATACG TTCGTGCCCA AGATTGCGAC CAGCACGCCC 
TCAAACGTAA TCAGCTCGAT CTCGGTAATT GGCAGCACTT ACGGCGCAAG CAGCAGCAGC 
AATGGCAGCC GAAGCACTAC TTCCACCGCA TCCTCCGCAC CAGCTGCTTC GCTTTTGGCG 
GAGACGGGCA GCCTGAAATC GCCAACTCCG CCGCTCGCCA CAGTTGATCC GGATCCGGAG 
CCCGTCGTGG AACAAAAGCC GGCGCTGCGG AATGCCACGC TGAAGCGGAC GAAAAAGGTG 
CAGAACGGCA TCGAAACGTC GGGCCTGTGC TCGATCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_001202002.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188931.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant E (Pcf11), mRNA.

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CloneID	OFa12552
Clone ID Related Accession (Same CDS sequence)	NM_001202002.2 , NM_001299501.1
Accession Version	NM_001202002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001202002.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGAACACC AGCGGCATCT 
CTAGTCAGGC AATCGTCGCC CAGTCCTTCG TCCTCGTCTA TTTCCTCGCA GGGTAATGTA 
TCGGACGCCC TGCAGCTGTC GATTAACAAG TTGCTCCAGG TGCAAGGCGG TGCCGGCGAG 
AAACGTCCCG CCGACGCACT GCCGCCACAT ATCGACGGTG AAGAGCAACC GCCGCAGCAA 
AAGCGCAGCA AGTCAGCCAA ACTGGACGCT CTCTTTGGCA GTGAGGATGT CGACCTGCGA 
CGCGAGATTC TAGTTGTCAA GCCAGGAGTT ATTGTTGTCG AAGACGACTC TATGGACGAA 
TGCGATGTAG ACAAGCCCAC AAAGAAATCT GGGTCGCCGC CCAAGAAGGC AACGTTGGAG 
GAGCTGCGCG CCAAGTTGGC TAACTCGGCT CGAATCCAGA ATAAGCAGGG AAAGTCTGAC 
AAATCCAGGG ATCAGGCCGT GTCACAACGC CTTAAGCAGC TGGCGGAGCT TAAAGTCAAC 
GACGATTCGC AGGAGGCGCA CGACGAGAAG GTGCGCACCA TTCTCAGCCA GGCGCAGGAG 
ATGTATGAGA ATAACGGCAT GAATCAGGAA CAGTACAAGG ATCTGGTCCA AAAAGTAGTG 
GCCATCAATG AGAATAGCAA AATAAAAGAG TCCCGGCGCC GAGACAACGA TCTCGAGCGC 
AATGCGGCCA GGGATGCAGT GCTACGCAAA CGTATTCCCA AGCTGAAGGG CAACGAAAAT 
CATTCTGCGG GCTCACCGCA TACCGATGGA TCGCCCAGGT ACGATGATCA GCCGGCAGCT 
GCCCCGGAGA AGCCCTCAAA CAAACGGGAT AAGACCAAGA GGGAGATGAA GCGTCGCAAG 
CCCACCAAAT GGGGCGAACA AGTGGATGCA GGGGCAGCAC AACGCACTGC CTGGCAACTG 
GCCAATGTCA ACAATAATAA TAATAATAAC AACAACAAGC GGGGCGGTAT CCAATTGCCC 
GTTCAGCCTC AGGCTGGATT CCGTGGCATG CCCTGGCAGC AGCCACCAGC CATGGTGTTG 
GGCCAGTCCG CAGTTCCGCC GCCACCGCAA CCGCCGTCGA TGATTAGTAT GCCTCCCGTG 
CCACCGGTGA CCATGACCAA GGCCATAAAT TCCCTTGACA ATCCAATGGC TGATGTAGTG 
CGAAGCATCA CGATCGACGG TGGCTCCAAG GAGATACGCT TCTACAACCA GGTGGCCATC 
ATTTTCATGG ATGGCGATCA GCCGCATGAA ATTGGCTTCC AGCCGGGTCA GCGGGTGATC 
TTCATTGATC ACAATGAACC GCTACCGCTT TGCTTCAACG ACGATTACAA GCCTTTCCAC 
TTGGATGGAC AGCTTCACCG GATTCGGTTT GGCTTTCCAT CGCGCGAACT TTATATCGAC 
GAGCACTGGT ACGAGATCTA CTTTGGCGGT CCAGCCGTAT CCGTGCCCAT TGGCAACAAA 
CTGCATATAA TCAAAGCTGA GGGTCCACCG CCCAACGTGG ACATTGGTCG AGTGCGAAGG 
GATCTGGTTG TGGGCAAAAT CAATATGATT GCGGATGCGC ATACTATAGT GCCACTCTTT 
CTGGACGCCA GACAGCAGAC CTTCCAGCTC GGCGCCGAGC AGCATTCACT GCAGTTCGTG 
GACAGTTTCC AATATGCTCT GCTCGACGGC CAGCTGCAGA AGATGGAGTA TGGTGGTCTC 
CCAAAAGGAA TGATGCTGAA CGGCGGTCGC AGCTGCTTCA TTAGGTTTGG AACGCTGCCC 
AAAGGAGTCA TTGCTGGAAA AACCCATGTG GCCGATATGG TGTACATTAA GACTGATGCT 
CCTGCAGAGC CTCCCAAGCC GCCGCCAATT ATTGTGAAAC CACCGCCAGT GGTGGAGCAA 
CCGAAGCCCG CTCCAGTTGC TGTTGCTCCC ATTTCCCTTC CAGCAGCAGC CGCCGCACTT 
GAGAGCTTAA ACATCAATGA TTTGTTTCAA AAGCTTGTCT CGTCTGGAAT AATTGGTGGT 
GCAACTGCAG CACCAACTCT CCCTGCCGCA GACTCATCAG CGGCCAAAGA GCCAACCGCT 
TCTGCCACCG AACCCTCAAC TGCATCTGCA ACCGTTGCGC CTGCTACACT GCCTGCTGGT 
CCCCCCGCGG AGCCGATCAA GCGCATCGAT CTCCGCAAGC CAGAGTCCAT CAAGACACGC 
CAGGCGGCTG TGGTGGCCGT TCTTTACCTG GGAATGCAGT GCAGCAGCTG TGGAGTTCGT 
TTCCCGCCAG AGCAAACAAT TAAGTACAGC CAACATTTGG ACTGGCACTT CCGACAGAAT 
CGTAGAGAGC GGGATTCCAC TAGGAAAGCC ACTTCGCGAA AATGGTATTA CGATCTCAAC 
GACTGGCGGC AATACGAAGA AATCGAAGAT GTGGAGGAGC GAGAGAAGAA CTTCCTCGAG 
GCTCAGGGAC AGCCAGGTGG CGTTGAGGCC CTTGATGAAC TCTCACAACA ACGCTCGCTG 
GATTCGCCCG TGCCAACATG TGCTGCTGGA AGGGATGATG TGGATCACTG CTGTGACATG 
TGTCACGAGA AATTCGAGCA GTTCTACAAC GAAGAGCTCG AGGAGTGGCA TCTGCGTAGC 
GCTATTCGTG TGGAAGATAA GATCTATCAT CCTCTATGCT ACGAGGACTA CAAGGCCTCG 
TTGAATCCTC CAACGGAGGT AAAGTCCGAC CAGGATGTGG ACATGAACAA CACCGATGAC 
AATGCCATGG ACACACTAAT CAAAGTGGAG GATGATGAAG ATGAAGGTAC GCCAAACTCA 
TCACAACCCA TTTTTGAAGA CGACGATGAC GATGTCATTG TGCTGCCCAA TGAAGAGCCA 
AGTGTCACGG AAATCGTCGA CGACGACGAT GAGGACGAAT ACGTTCCGGG CAATGTGACA 
CGTGCGGACA TGGGCAACGA GTCACAGGAC AAAACAGAGT CCAACTCCGA GACCAAAGAA 
CCGAAGAAAG ATCAGGTTGA GCTCGGTGAA CAGCAGCAAA ACGAATCGGC CAATGAATCG 
GATGTCGAAA TCCAAGAGCC GAACATTCCT TTCACGGATC TGGACACTTA TGTGGAGAAA 
GAGATGGATG AGGCGACCAG GGCGGCGCTA CTGAACGTAA AGATCAAGGA GGAGCCCAAG 
GATGAGTATG AGGAGGACGA GGATGATGGA TTCGAAGACG TGGGCACGGT GGTTTCACTT 
TTACCTCTGC CCGAGGACGA GATCTCCATA CACAGCAGCG AAACTCAAAC ACACACCATC 
GGCTCGTCAG CCTCGCCGGC CACCATCGAG CGGCCAGCAT CGGTGACCTC GCTCTCCCTG 
CCCGCCAATG AGGCGGACGA GTTGGAGCAG GGTGATGCAA ACACCGATAC GGATGCGGAA 
TTGAATGGCG AGAAGCAGGA GGCCACGCAT AACCTGAGCG CAGTGGGTCC GGCGTTACCT 
TTGGCTAGCA TAGTTAATAA AATAAAGATA AATATCACTA AGAATACGAG TAGTAATAGT 
CATAATAGTG CCTCCAACAC CACGACAACG GACTCGCAAG TCTCGGCCAT AAGCGTCATC 
GGTGGATCAG GAGCAGCTGG CGGTGCATCC GGAGCAGGAG ACCCTGTCCA GCAGGTGAAT 
GCCATTCAAA CCATTTCCAC CATTCCAGTG CTGTGCGGCG GCAATACGTT CGTGCCCAAG 
ATTGCGACCA GCACGCCCTC AAACGTAATC AGCTCGATCT CGGTAATTGG CAGCACTTAC 
GGCGCAAGCA GCAGCAGCAA TGGCAGCCGA AGCACTACTT CCACCGCATC CTCCGCACCA 
GCTGCTTCGC TTTTGGCGGA GACGGGCAGC CTGAAATCGC CAACTCCGCC GCTCGCCACA 
GTTGATCCGG ATCCGGAGCC CGTCGTGGAA CAAAAGCCGG CGCTGCGGAA TGCCACGCTG 
AAGCGGACGA AAAAGGTGCA GAACGGCATC GAAACGTCGG GCCTGTGCTC GATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188931.1
CDS	234..5753
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKRTPAASLVRQSSPSPSSSSISSQGNVSDALQLSINKLLQVQGGAGEKRPADALPPHIDGEEQPPQQKRSKSAKLDALFGSEDVDLRREILVVKPGVIVVEDDSMDECDVDKPTKKSGSPPKKATLEELRAKLANSARIQNKQGKSDKSRDQAVSQRLKQLAELKVNDDSQEAHDEKVRTILSQAQEMYENNGMNQEQYKDLVQKVVAINENSKIKESRRRDNDLERNAARDAVLRKRIPKLKGNENHSAGSPHTDGSPRYDDQPAAAPEKPSNKRDKTKREMKRRKPTKWGEQVDAGAAQRTAWQLANVNNNNNNNNNKRGGIQLPVQPQAGFRGMPWQQPPAMVLGQSAVPPPPQPPSMISMPPVPPVTMTKAINSLDNPMADVVRSITIDGGSKEIRFYNQVAIIFMDGDQPHEIGFQPGQRVIFIDHNEPLPLCFNDDYKPFHLDGQLHRIRFGFPSRELYIDEHWYEIYFGGPAVSVPIGNKLHIIKAEGPPPNVDIGRVRRDLVVGKINMIADAHTIVPLFLDARQQTFQLGAEQHSLQFVDSFQYALLDGQLQKMEYGGLPKGMMLNGGRSCFIRFGTLPKGVIAGKTHVADMVYIKTDAPAEPPKPPPIIVKPPPVVEQPKPAPVAVAPISLPAAAAALESLNINDLFQKLVSSGIIGGATAAPTLPAADSSAAKEPTASATEPSTASATVAPATLPAGPPAEPIKRIDLRKPESIKTRQAAVVAVLYLGMQCSSCGVRFPPEQTIKYSQHLDWHFRQNRRERDSTRKATSRKWYYDLNDWRQYEEIEDVEEREKNFLEAQGQPGGVEALDELSQQRSLDSPVPTCAAGRDDVDHCCDMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCYEDYKASLNPPTEVKSDQDVDMNNTDDNAMDTLIKVEDDEDEGTPNSSQPIFEDDDDDVIVLPNEEPSVTEIVDDDDEDEYVPGNVTRADMGNESQDKTESNSETKEPKKDQVELGEQQQNESANESDVEIQEPNIPFTDLDTYVEKEMDEATRAALLNVKIKEEPKDEYEEDEDDGFEDVGTVVSLLPLPEDEISIHSSETQTHTIGSSASPATIERPASVTSLSLPANEADELEQGDANTDTDAELNGEKQEATHNLSAVGPALPLASIVNKIKINITKNTSSNSHNSASNTTTTDSQVSAISVIGGSGAAGGASGAGDPVQQVNAIQTISTIPVLCGGNTFVPKIATSTPSNVISSISVIGSTYGASSSSNGSRSTTSTASSAPAASLLAETGSLKSPTPPLATVDPDPEPVVEQKPALRNATLKRTKKVQNGIETSGLCSIM

Target ORF information:

RefSeq Version	NM_001202002.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant E (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202002.2

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGAACACC AGCGGCATCT 
CTAGTCAGGC AATCGTCGCC CAGTCCTTCG TCCTCGTCTA TTTCCTCGCA GGGTAATGTA 
TCGGACGCCC TGCAGCTGTC GATTAACAAG TTGCTCCAGG TGCAAGGCGG TGCCGGCGAG 
AAACGTCCCG CCGACGCACT GCCGCCACAT ATCGACGGTG AAGAGCAACC GCCGCAGCAA 
AAGCGCAGCA AGTCAGCCAA ACTGGACGCT CTCTTTGGCA GTGAGGATGT CGACCTGCGA 
CGCGAGATTC TAGTTGTCAA GCCAGGAGTT ATTGTTGTCG AAGACGACTC TATGGACGAA 
TGCGATGTAG ACAAGCCCAC AAAGAAATCT GGGTCGCCGC CCAAGAAGGC AACGTTGGAG 
GAGCTGCGCG CCAAGTTGGC TAACTCGGCT CGAATCCAGA ATAAGCAGGG AAAGTCTGAC 
AAATCCAGGG ATCAGGCCGT GTCACAACGC CTTAAGCAGC TGGCGGAGCT TAAAGTCAAC 
GACGATTCGC AGGAGGCGCA CGACGAGAAG GTGCGCACCA TTCTCAGCCA GGCGCAGGAG 
ATGTATGAGA ATAACGGCAT GAATCAGGAA CAGTACAAGG ATCTGGTCCA AAAAGTAGTG 
GCCATCAATG AGAATAGCAA AATAAAAGAG TCCCGGCGCC GAGACAACGA TCTCGAGCGC 
AATGCGGCCA GGGATGCAGT GCTACGCAAA CGTATTCCCA AGCTGAAGGG CAACGAAAAT 
CATTCTGCGG GCTCACCGCA TACCGATGGA TCGCCCAGGT ACGATGATCA GCCGGCAGCT 
GCCCCGGAGA AGCCCTCAAA CAAACGGGAT AAGACCAAGA GGGAGATGAA GCGTCGCAAG 
CCCACCAAAT GGGGCGAACA AGTGGATGCA GGGGCAGCAC AACGCACTGC CTGGCAACTG 
GCCAATGTCA ACAATAATAA TAATAATAAC AACAACAAGC GGGGCGGTAT CCAATTGCCC 
GTTCAGCCTC AGGCTGGATT CCGTGGCATG CCCTGGCAGC AGCCACCAGC CATGGTGTTG 
GGCCAGTCCG CAGTTCCGCC GCCACCGCAA CCGCCGTCGA TGATTAGTAT GCCTCCCGTG 
CCACCGGTGA CCATGACCAA GGCCATAAAT TCCCTTGACA ATCCAATGGC TGATGTAGTG 
CGAAGCATCA CGATCGACGG TGGCTCCAAG GAGATACGCT TCTACAACCA GGTGGCCATC 
ATTTTCATGG ATGGCGATCA GCCGCATGAA ATTGGCTTCC AGCCGGGTCA GCGGGTGATC 
TTCATTGATC ACAATGAACC GCTACCGCTT TGCTTCAACG ACGATTACAA GCCTTTCCAC 
TTGGATGGAC AGCTTCACCG GATTCGGTTT GGCTTTCCAT CGCGCGAACT TTATATCGAC 
GAGCACTGGT ACGAGATCTA CTTTGGCGGT CCAGCCGTAT CCGTGCCCAT TGGCAACAAA 
CTGCATATAA TCAAAGCTGA GGGTCCACCG CCCAACGTGG ACATTGGTCG AGTGCGAAGG 
GATCTGGTTG TGGGCAAAAT CAATATGATT GCGGATGCGC ATACTATAGT GCCACTCTTT 
CTGGACGCCA GACAGCAGAC CTTCCAGCTC GGCGCCGAGC AGCATTCACT GCAGTTCGTG 
GACAGTTTCC AATATGCTCT GCTCGACGGC CAGCTGCAGA AGATGGAGTA TGGTGGTCTC 
CCAAAAGGAA TGATGCTGAA CGGCGGTCGC AGCTGCTTCA TTAGGTTTGG AACGCTGCCC 
AAAGGAGTCA TTGCTGGAAA AACCCATGTG GCCGATATGG TGTACATTAA GACTGATGCT 
CCTGCAGAGC CTCCCAAGCC GCCGCCAATT ATTGTGAAAC CACCGCCAGT GGTGGAGCAA 
CCGAAGCCCG CTCCAGTTGC TGTTGCTCCC ATTTCCCTTC CAGCAGCAGC CGCCGCACTT 
GAGAGCTTAA ACATCAATGA TTTGTTTCAA AAGCTTGTCT CGTCTGGAAT AATTGGTGGT 
GCAACTGCAG CACCAACTCT CCCTGCCGCA GACTCATCAG CGGCCAAAGA GCCAACCGCT 
TCTGCCACCG AACCCTCAAC TGCATCTGCA ACCGTTGCGC CTGCTACACT GCCTGCTGGT 
CCCCCCGCGG AGCCGATCAA GCGCATCGAT CTCCGCAAGC CAGAGTCCAT CAAGACACGC 
CAGGCGGCTG TGGTGGCCGT TCTTTACCTG GGAATGCAGT GCAGCAGCTG TGGAGTTCGT 
TTCCCGCCAG AGCAAACAAT TAAGTACAGC CAACATTTGG ACTGGCACTT CCGACAGAAT 
CGTAGAGAGC GGGATTCCAC TAGGAAAGCC ACTTCGCGAA AATGGTATTA CGATCTCAAC 
GACTGGCGGC AATACGAAGA AATCGAAGAT GTGGAGGAGC GAGAGAAGAA CTTCCTCGAG 
GCTCAGGGAC AGCCAGGTGG CGTTGAGGCC CTTGATGAAC TCTCACAACA ACGCTCGCTG 
GATTCGCCCG TGCCAACATG TGCTGCTGGA AGGGATGATG TGGATCACTG CTGTGACATG 
TGTCACGAGA AATTCGAGCA GTTCTACAAC GAAGAGCTCG AGGAGTGGCA TCTGCGTAGC 
GCTATTCGTG TGGAAGATAA GATCTATCAT CCTCTATGCT ACGAGGACTA CAAGGCCTCG 
TTGAATCCTC CAACGGAGGT AAAGTCCGAC CAGGATGTGG ACATGAACAA CACCGATGAC 
AATGCCATGG ACACACTAAT CAAAGTGGAG GATGATGAAG ATGAAGGTAC GCCAAACTCA 
TCACAACCCA TTTTTGAAGA CGACGATGAC GATGTCATTG TGCTGCCCAA TGAAGAGCCA 
AGTGTCACGG AAATCGTCGA CGACGACGAT GAGGACGAAT ACGTTCCGGG CAATGTGACA 
CGTGCGGACA TGGGCAACGA GTCACAGGAC AAAACAGAGT CCAACTCCGA GACCAAAGAA 
CCGAAGAAAG ATCAGGTTGA GCTCGGTGAA CAGCAGCAAA ACGAATCGGC CAATGAATCG 
GATGTCGAAA TCCAAGAGCC GAACATTCCT TTCACGGATC TGGACACTTA TGTGGAGAAA 
GAGATGGATG AGGCGACCAG GGCGGCGCTA CTGAACGTAA AGATCAAGGA GGAGCCCAAG 
GATGAGTATG AGGAGGACGA GGATGATGGA TTCGAAGACG TGGGCACGGT GGTTTCACTT 
TTACCTCTGC CCGAGGACGA GATCTCCATA CACAGCAGCG AAACTCAAAC ACACACCATC 
GGCTCGTCAG CCTCGCCGGC CACCATCGAG CGGCCAGCAT CGGTGACCTC GCTCTCCCTG 
CCCGCCAATG AGGCGGACGA GTTGGAGCAG GGTGATGCAA ACACCGATAC GGATGCGGAA 
TTGAATGGCG AGAAGCAGGA GGCCACGCAT AACCTGAGCG CAGTGGGTCC GGCGTTACCT 
TTGGCTAGCA TAGTTAATAA AATAAAGATA AATATCACTA AGAATACGAG TAGTAATAGT 
CATAATAGTG CCTCCAACAC CACGACAACG GACTCGCAAG TCTCGGCCAT AAGCGTCATC 
GGTGGATCAG GAGCAGCTGG CGGTGCATCC GGAGCAGGAG ACCCTGTCCA GCAGGTGAAT 
GCCATTCAAA CCATTTCCAC CATTCCAGTG CTGTGCGGCG GCAATACGTT CGTGCCCAAG 
ATTGCGACCA GCACGCCCTC AAACGTAATC AGCTCGATCT CGGTAATTGG CAGCACTTAC 
GGCGCAAGCA GCAGCAGCAA TGGCAGCCGA AGCACTACTT CCACCGCATC CTCCGCACCA 
GCTGCTTCGC TTTTGGCGGA GACGGGCAGC CTGAAATCGC CAACTCCGCC GCTCGCCACA 
GTTGATCCGG ATCCGGAGCC CGTCGTGGAA CAAAAGCCGG CGCTGCGGAA TGCCACGCTG 
AAGCGGACGA AAAAGGTGCA GAACGGCATC GAAACGTCGG GCCTGTGCTC GATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_001299501.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286430.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant H (Pcf11), mRNA.

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CloneID	OFa12552
Clone ID Related Accession (Same CDS sequence)	NM_001202002.2 , NM_001299501.1
Accession Version	NM_001299501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGAACACC AGCGGCATCT 
CTAGTCAGGC AATCGTCGCC CAGTCCTTCG TCCTCGTCTA TTTCCTCGCA GGGTAATGTA 
TCGGACGCCC TGCAGCTGTC GATTAACAAG TTGCTCCAGG TGCAAGGCGG TGCCGGCGAG 
AAACGTCCCG CCGACGCACT GCCGCCACAT ATCGACGGTG AAGAGCAACC GCCGCAGCAA 
AAGCGCAGCA AGTCAGCCAA ACTGGACGCT CTCTTTGGCA GTGAGGATGT CGACCTGCGA 
CGCGAGATTC TAGTTGTCAA GCCAGGAGTT ATTGTTGTCG AAGACGACTC TATGGACGAA 
TGCGATGTAG ACAAGCCCAC AAAGAAATCT GGGTCGCCGC CCAAGAAGGC AACGTTGGAG 
GAGCTGCGCG CCAAGTTGGC TAACTCGGCT CGAATCCAGA ATAAGCAGGG AAAGTCTGAC 
AAATCCAGGG ATCAGGCCGT GTCACAACGC CTTAAGCAGC TGGCGGAGCT TAAAGTCAAC 
GACGATTCGC AGGAGGCGCA CGACGAGAAG GTGCGCACCA TTCTCAGCCA GGCGCAGGAG 
ATGTATGAGA ATAACGGCAT GAATCAGGAA CAGTACAAGG ATCTGGTCCA AAAAGTAGTG 
GCCATCAATG AGAATAGCAA AATAAAAGAG TCCCGGCGCC GAGACAACGA TCTCGAGCGC 
AATGCGGCCA GGGATGCAGT GCTACGCAAA CGTATTCCCA AGCTGAAGGG CAACGAAAAT 
CATTCTGCGG GCTCACCGCA TACCGATGGA TCGCCCAGGT ACGATGATCA GCCGGCAGCT 
GCCCCGGAGA AGCCCTCAAA CAAACGGGAT AAGACCAAGA GGGAGATGAA GCGTCGCAAG 
CCCACCAAAT GGGGCGAACA AGTGGATGCA GGGGCAGCAC AACGCACTGC CTGGCAACTG 
GCCAATGTCA ACAATAATAA TAATAATAAC AACAACAAGC GGGGCGGTAT CCAATTGCCC 
GTTCAGCCTC AGGCTGGATT CCGTGGCATG CCCTGGCAGC AGCCACCAGC CATGGTGTTG 
GGCCAGTCCG CAGTTCCGCC GCCACCGCAA CCGCCGTCGA TGATTAGTAT GCCTCCCGTG 
CCACCGGTGA CCATGACCAA GGCCATAAAT TCCCTTGACA ATCCAATGGC TGATGTAGTG 
CGAAGCATCA CGATCGACGG TGGCTCCAAG GAGATACGCT TCTACAACCA GGTGGCCATC 
ATTTTCATGG ATGGCGATCA GCCGCATGAA ATTGGCTTCC AGCCGGGTCA GCGGGTGATC 
TTCATTGATC ACAATGAACC GCTACCGCTT TGCTTCAACG ACGATTACAA GCCTTTCCAC 
TTGGATGGAC AGCTTCACCG GATTCGGTTT GGCTTTCCAT CGCGCGAACT TTATATCGAC 
GAGCACTGGT ACGAGATCTA CTTTGGCGGT CCAGCCGTAT CCGTGCCCAT TGGCAACAAA 
CTGCATATAA TCAAAGCTGA GGGTCCACCG CCCAACGTGG ACATTGGTCG AGTGCGAAGG 
GATCTGGTTG TGGGCAAAAT CAATATGATT GCGGATGCGC ATACTATAGT GCCACTCTTT 
CTGGACGCCA GACAGCAGAC CTTCCAGCTC GGCGCCGAGC AGCATTCACT GCAGTTCGTG 
GACAGTTTCC AATATGCTCT GCTCGACGGC CAGCTGCAGA AGATGGAGTA TGGTGGTCTC 
CCAAAAGGAA TGATGCTGAA CGGCGGTCGC AGCTGCTTCA TTAGGTTTGG AACGCTGCCC 
AAAGGAGTCA TTGCTGGAAA AACCCATGTG GCCGATATGG TGTACATTAA GACTGATGCT 
CCTGCAGAGC CTCCCAAGCC GCCGCCAATT ATTGTGAAAC CACCGCCAGT GGTGGAGCAA 
CCGAAGCCCG CTCCAGTTGC TGTTGCTCCC ATTTCCCTTC CAGCAGCAGC CGCCGCACTT 
GAGAGCTTAA ACATCAATGA TTTGTTTCAA AAGCTTGTCT CGTCTGGAAT AATTGGTGGT 
GCAACTGCAG CACCAACTCT CCCTGCCGCA GACTCATCAG CGGCCAAAGA GCCAACCGCT 
TCTGCCACCG AACCCTCAAC TGCATCTGCA ACCGTTGCGC CTGCTACACT GCCTGCTGGT 
CCCCCCGCGG AGCCGATCAA GCGCATCGAT CTCCGCAAGC CAGAGTCCAT CAAGACACGC 
CAGGCGGCTG TGGTGGCCGT TCTTTACCTG GGAATGCAGT GCAGCAGCTG TGGAGTTCGT 
TTCCCGCCAG AGCAAACAAT TAAGTACAGC CAACATTTGG ACTGGCACTT CCGACAGAAT 
CGTAGAGAGC GGGATTCCAC TAGGAAAGCC ACTTCGCGAA AATGGTATTA CGATCTCAAC 
GACTGGCGGC AATACGAAGA AATCGAAGAT GTGGAGGAGC GAGAGAAGAA CTTCCTCGAG 
GCTCAGGGAC AGCCAGGTGG CGTTGAGGCC CTTGATGAAC TCTCACAACA ACGCTCGCTG 
GATTCGCCCG TGCCAACATG TGCTGCTGGA AGGGATGATG TGGATCACTG CTGTGACATG 
TGTCACGAGA AATTCGAGCA GTTCTACAAC GAAGAGCTCG AGGAGTGGCA TCTGCGTAGC 
GCTATTCGTG TGGAAGATAA GATCTATCAT CCTCTATGCT ACGAGGACTA CAAGGCCTCG 
TTGAATCCTC CAACGGAGGT AAAGTCCGAC CAGGATGTGG ACATGAACAA CACCGATGAC 
AATGCCATGG ACACACTAAT CAAAGTGGAG GATGATGAAG ATGAAGGTAC GCCAAACTCA 
TCACAACCCA TTTTTGAAGA CGACGATGAC GATGTCATTG TGCTGCCCAA TGAAGAGCCA 
AGTGTCACGG AAATCGTCGA CGACGACGAT GAGGACGAAT ACGTTCCGGG CAATGTGACA 
CGTGCGGACA TGGGCAACGA GTCACAGGAC AAAACAGAGT CCAACTCCGA GACCAAAGAA 
CCGAAGAAAG ATCAGGTTGA GCTCGGTGAA CAGCAGCAAA ACGAATCGGC CAATGAATCG 
GATGTCGAAA TCCAAGAGCC GAACATTCCT TTCACGGATC TGGACACTTA TGTGGAGAAA 
GAGATGGATG AGGCGACCAG GGCGGCGCTA CTGAACGTAA AGATCAAGGA GGAGCCCAAG 
GATGAGTATG AGGAGGACGA GGATGATGGA TTCGAAGACG TGGGCACGGT GGTTTCACTT 
TTACCTCTGC CCGAGGACGA GATCTCCATA CACAGCAGCG AAACTCAAAC ACACACCATC 
GGCTCGTCAG CCTCGCCGGC CACCATCGAG CGGCCAGCAT CGGTGACCTC GCTCTCCCTG 
CCCGCCAATG AGGCGGACGA GTTGGAGCAG GGTGATGCAA ACACCGATAC GGATGCGGAA 
TTGAATGGCG AGAAGCAGGA GGCCACGCAT AACCTGAGCG CAGTGGGTCC GGCGTTACCT 
TTGGCTAGCA TAGTTAATAA AATAAAGATA AATATCACTA AGAATACGAG TAGTAATAGT 
CATAATAGTG CCTCCAACAC CACGACAACG GACTCGCAAG TCTCGGCCAT AAGCGTCATC 
GGTGGATCAG GAGCAGCTGG CGGTGCATCC GGAGCAGGAG ACCCTGTCCA GCAGGTGAAT 
GCCATTCAAA CCATTTCCAC CATTCCAGTG CTGTGCGGCG GCAATACGTT CGTGCCCAAG 
ATTGCGACCA GCACGCCCTC AAACGTAATC AGCTCGATCT CGGTAATTGG CAGCACTTAC 
GGCGCAAGCA GCAGCAGCAA TGGCAGCCGA AGCACTACTT CCACCGCATC CTCCGCACCA 
GCTGCTTCGC TTTTGGCGGA GACGGGCAGC CTGAAATCGC CAACTCCGCC GCTCGCCACA 
GTTGATCCGG ATCCGGAGCC CGTCGTGGAA CAAAAGCCGG CGCTGCGGAA TGCCACGCTG 
AAGCGGACGA AAAAGGTGCA GAACGGCATC GAAACGTCGG GCCTGTGCTC GATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286430.1
CDS	234..5753
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKRTPAASLVRQSSPSPSSSSISSQGNVSDALQLSINKLLQVQGGAGEKRPADALPPHIDGEEQPPQQKRSKSAKLDALFGSEDVDLRREILVVKPGVIVVEDDSMDECDVDKPTKKSGSPPKKATLEELRAKLANSARIQNKQGKSDKSRDQAVSQRLKQLAELKVNDDSQEAHDEKVRTILSQAQEMYENNGMNQEQYKDLVQKVVAINENSKIKESRRRDNDLERNAARDAVLRKRIPKLKGNENHSAGSPHTDGSPRYDDQPAAAPEKPSNKRDKTKREMKRRKPTKWGEQVDAGAAQRTAWQLANVNNNNNNNNNKRGGIQLPVQPQAGFRGMPWQQPPAMVLGQSAVPPPPQPPSMISMPPVPPVTMTKAINSLDNPMADVVRSITIDGGSKEIRFYNQVAIIFMDGDQPHEIGFQPGQRVIFIDHNEPLPLCFNDDYKPFHLDGQLHRIRFGFPSRELYIDEHWYEIYFGGPAVSVPIGNKLHIIKAEGPPPNVDIGRVRRDLVVGKINMIADAHTIVPLFLDARQQTFQLGAEQHSLQFVDSFQYALLDGQLQKMEYGGLPKGMMLNGGRSCFIRFGTLPKGVIAGKTHVADMVYIKTDAPAEPPKPPPIIVKPPPVVEQPKPAPVAVAPISLPAAAAALESLNINDLFQKLVSSGIIGGATAAPTLPAADSSAAKEPTASATEPSTASATVAPATLPAGPPAEPIKRIDLRKPESIKTRQAAVVAVLYLGMQCSSCGVRFPPEQTIKYSQHLDWHFRQNRRERDSTRKATSRKWYYDLNDWRQYEEIEDVEEREKNFLEAQGQPGGVEALDELSQQRSLDSPVPTCAAGRDDVDHCCDMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCYEDYKASLNPPTEVKSDQDVDMNNTDDNAMDTLIKVEDDEDEGTPNSSQPIFEDDDDDVIVLPNEEPSVTEIVDDDDEDEYVPGNVTRADMGNESQDKTESNSETKEPKKDQVELGEQQQNESANESDVEIQEPNIPFTDLDTYVEKEMDEATRAALLNVKIKEEPKDEYEEDEDDGFEDVGTVVSLLPLPEDEISIHSSETQTHTIGSSASPATIERPASVTSLSLPANEADELEQGDANTDTDAELNGEKQEATHNLSAVGPALPLASIVNKIKINITKNTSSNSHNSASNTTTTDSQVSAISVIGGSGAAGGASGAGDPVQQVNAIQTISTIPVLCGGNTFVPKIATSTPSNVISSISVIGSTYGASSSSNGSRSTTSTASSAPAASLLAETGSLKSPTPPLATVDPDPEPVVEQKPALRNATLKRTKKVQNGIETSGLCSIM

Target ORF information:

RefSeq Version	NM_001299501.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant H (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299501.1

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGAACACC AGCGGCATCT 
CTAGTCAGGC AATCGTCGCC CAGTCCTTCG TCCTCGTCTA TTTCCTCGCA GGGTAATGTA 
TCGGACGCCC TGCAGCTGTC GATTAACAAG TTGCTCCAGG TGCAAGGCGG TGCCGGCGAG 
AAACGTCCCG CCGACGCACT GCCGCCACAT ATCGACGGTG AAGAGCAACC GCCGCAGCAA 
AAGCGCAGCA AGTCAGCCAA ACTGGACGCT CTCTTTGGCA GTGAGGATGT CGACCTGCGA 
CGCGAGATTC TAGTTGTCAA GCCAGGAGTT ATTGTTGTCG AAGACGACTC TATGGACGAA 
TGCGATGTAG ACAAGCCCAC AAAGAAATCT GGGTCGCCGC CCAAGAAGGC AACGTTGGAG 
GAGCTGCGCG CCAAGTTGGC TAACTCGGCT CGAATCCAGA ATAAGCAGGG AAAGTCTGAC 
AAATCCAGGG ATCAGGCCGT GTCACAACGC CTTAAGCAGC TGGCGGAGCT TAAAGTCAAC 
GACGATTCGC AGGAGGCGCA CGACGAGAAG GTGCGCACCA TTCTCAGCCA GGCGCAGGAG 
ATGTATGAGA ATAACGGCAT GAATCAGGAA CAGTACAAGG ATCTGGTCCA AAAAGTAGTG 
GCCATCAATG AGAATAGCAA AATAAAAGAG TCCCGGCGCC GAGACAACGA TCTCGAGCGC 
AATGCGGCCA GGGATGCAGT GCTACGCAAA CGTATTCCCA AGCTGAAGGG CAACGAAAAT 
CATTCTGCGG GCTCACCGCA TACCGATGGA TCGCCCAGGT ACGATGATCA GCCGGCAGCT 
GCCCCGGAGA AGCCCTCAAA CAAACGGGAT AAGACCAAGA GGGAGATGAA GCGTCGCAAG 
CCCACCAAAT GGGGCGAACA AGTGGATGCA GGGGCAGCAC AACGCACTGC CTGGCAACTG 
GCCAATGTCA ACAATAATAA TAATAATAAC AACAACAAGC GGGGCGGTAT CCAATTGCCC 
GTTCAGCCTC AGGCTGGATT CCGTGGCATG CCCTGGCAGC AGCCACCAGC CATGGTGTTG 
GGCCAGTCCG CAGTTCCGCC GCCACCGCAA CCGCCGTCGA TGATTAGTAT GCCTCCCGTG 
CCACCGGTGA CCATGACCAA GGCCATAAAT TCCCTTGACA ATCCAATGGC TGATGTAGTG 
CGAAGCATCA CGATCGACGG TGGCTCCAAG GAGATACGCT TCTACAACCA GGTGGCCATC 
ATTTTCATGG ATGGCGATCA GCCGCATGAA ATTGGCTTCC AGCCGGGTCA GCGGGTGATC 
TTCATTGATC ACAATGAACC GCTACCGCTT TGCTTCAACG ACGATTACAA GCCTTTCCAC 
TTGGATGGAC AGCTTCACCG GATTCGGTTT GGCTTTCCAT CGCGCGAACT TTATATCGAC 
GAGCACTGGT ACGAGATCTA CTTTGGCGGT CCAGCCGTAT CCGTGCCCAT TGGCAACAAA 
CTGCATATAA TCAAAGCTGA GGGTCCACCG CCCAACGTGG ACATTGGTCG AGTGCGAAGG 
GATCTGGTTG TGGGCAAAAT CAATATGATT GCGGATGCGC ATACTATAGT GCCACTCTTT 
CTGGACGCCA GACAGCAGAC CTTCCAGCTC GGCGCCGAGC AGCATTCACT GCAGTTCGTG 
GACAGTTTCC AATATGCTCT GCTCGACGGC CAGCTGCAGA AGATGGAGTA TGGTGGTCTC 
CCAAAAGGAA TGATGCTGAA CGGCGGTCGC AGCTGCTTCA TTAGGTTTGG AACGCTGCCC 
AAAGGAGTCA TTGCTGGAAA AACCCATGTG GCCGATATGG TGTACATTAA GACTGATGCT 
CCTGCAGAGC CTCCCAAGCC GCCGCCAATT ATTGTGAAAC CACCGCCAGT GGTGGAGCAA 
CCGAAGCCCG CTCCAGTTGC TGTTGCTCCC ATTTCCCTTC CAGCAGCAGC CGCCGCACTT 
GAGAGCTTAA ACATCAATGA TTTGTTTCAA AAGCTTGTCT CGTCTGGAAT AATTGGTGGT 
GCAACTGCAG CACCAACTCT CCCTGCCGCA GACTCATCAG CGGCCAAAGA GCCAACCGCT 
TCTGCCACCG AACCCTCAAC TGCATCTGCA ACCGTTGCGC CTGCTACACT GCCTGCTGGT 
CCCCCCGCGG AGCCGATCAA GCGCATCGAT CTCCGCAAGC CAGAGTCCAT CAAGACACGC 
CAGGCGGCTG TGGTGGCCGT TCTTTACCTG GGAATGCAGT GCAGCAGCTG TGGAGTTCGT 
TTCCCGCCAG AGCAAACAAT TAAGTACAGC CAACATTTGG ACTGGCACTT CCGACAGAAT 
CGTAGAGAGC GGGATTCCAC TAGGAAAGCC ACTTCGCGAA AATGGTATTA CGATCTCAAC 
GACTGGCGGC AATACGAAGA AATCGAAGAT GTGGAGGAGC GAGAGAAGAA CTTCCTCGAG 
GCTCAGGGAC AGCCAGGTGG CGTTGAGGCC CTTGATGAAC TCTCACAACA ACGCTCGCTG 
GATTCGCCCG TGCCAACATG TGCTGCTGGA AGGGATGATG TGGATCACTG CTGTGACATG 
TGTCACGAGA AATTCGAGCA GTTCTACAAC GAAGAGCTCG AGGAGTGGCA TCTGCGTAGC 
GCTATTCGTG TGGAAGATAA GATCTATCAT CCTCTATGCT ACGAGGACTA CAAGGCCTCG 
TTGAATCCTC CAACGGAGGT AAAGTCCGAC CAGGATGTGG ACATGAACAA CACCGATGAC 
AATGCCATGG ACACACTAAT CAAAGTGGAG GATGATGAAG ATGAAGGTAC GCCAAACTCA 
TCACAACCCA TTTTTGAAGA CGACGATGAC GATGTCATTG TGCTGCCCAA TGAAGAGCCA 
AGTGTCACGG AAATCGTCGA CGACGACGAT GAGGACGAAT ACGTTCCGGG CAATGTGACA 
CGTGCGGACA TGGGCAACGA GTCACAGGAC AAAACAGAGT CCAACTCCGA GACCAAAGAA 
CCGAAGAAAG ATCAGGTTGA GCTCGGTGAA CAGCAGCAAA ACGAATCGGC CAATGAATCG 
GATGTCGAAA TCCAAGAGCC GAACATTCCT TTCACGGATC TGGACACTTA TGTGGAGAAA 
GAGATGGATG AGGCGACCAG GGCGGCGCTA CTGAACGTAA AGATCAAGGA GGAGCCCAAG 
GATGAGTATG AGGAGGACGA GGATGATGGA TTCGAAGACG TGGGCACGGT GGTTTCACTT 
TTACCTCTGC CCGAGGACGA GATCTCCATA CACAGCAGCG AAACTCAAAC ACACACCATC 
GGCTCGTCAG CCTCGCCGGC CACCATCGAG CGGCCAGCAT CGGTGACCTC GCTCTCCCTG 
CCCGCCAATG AGGCGGACGA GTTGGAGCAG GGTGATGCAA ACACCGATAC GGATGCGGAA 
TTGAATGGCG AGAAGCAGGA GGCCACGCAT AACCTGAGCG CAGTGGGTCC GGCGTTACCT 
TTGGCTAGCA TAGTTAATAA AATAAAGATA AATATCACTA AGAATACGAG TAGTAATAGT 
CATAATAGTG CCTCCAACAC CACGACAACG GACTCGCAAG TCTCGGCCAT AAGCGTCATC 
GGTGGATCAG GAGCAGCTGG CGGTGCATCC GGAGCAGGAG ACCCTGTCCA GCAGGTGAAT 
GCCATTCAAA CCATTTCCAC CATTCCAGTG CTGTGCGGCG GCAATACGTT CGTGCCCAAG 
ATTGCGACCA GCACGCCCTC AAACGTAATC AGCTCGATCT CGGTAATTGG CAGCACTTAC 
GGCGCAAGCA GCAGCAGCAA TGGCAGCCGA AGCACTACTT CCACCGCATC CTCCGCACCA 
GCTGCTTCGC TTTTGGCGGA GACGGGCAGC CTGAAATCGC CAACTCCGCC GCTCGCCACA 
GTTGATCCGG ATCCGGAGCC CGTCGTGGAA CAAAAGCCGG CGCTGCGGAA TGCCACGCTG 
AAGCGGACGA AAAAGGTGCA GAACGGCATC GAAACGTCGG GCCTGTGCTC GATCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_001299499.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286428.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant F (Pcf11), mRNA.

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CloneID	OFa21809
Clone ID Related Accession (Same CDS sequence)	NM_001299499.1
Accession Version	NM_001299499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACTATCAAT GCTCAACTCA 
AATCCCACGA CTTCAGGAAC ACCAGCGGCA TCTCTAGTCA GGCAATCGTC GCCCAGTCCT 
TCGTCCTCGT CTATTTCCTC GCAGGGTAAT GTATCGGACG CCCTGCAGCT GTCGATTAAC 
AAGTTGCTCC AGGTGCAAGG CGGTGCCGGC GAGAAACGTC CCGCCGACGC ACTGCCGCCA 
CATATCGACG GTGAAGAGCA ACCGCCGCAG CAAAAGCGCA GCAAGTCAGC CAAACTGGAC 
GCTCTCTTTG GCAGTGAGGA TGTCGACCTG CGACGCGAGA TTCTAGTTGT CAAGCCAGGA 
GTTATTGTTG TCGAAGACGA CTCTATGGAC GAATGCGATG TAGACAAGCC CACAAAGAAA 
TCTGGGTCGC CGCCCAAGAA GGCAACGTTG GAGGAGCTGC GCGCCAAGTT GGCTAACTCG 
GCTCGAATCC AGAATAAGCA GGGAAAGTCT GACAAATCCA GGGATCAGGC CGTGTCACAA 
CGCCTTAAGC AGCTGGCGGA GCTTAAAGTC AACGACGATT CGCAGGAGGC GCACGACGAG 
AAGGTGCGCA CCATTCTCAG CCAGGCGCAG GAGATGTATG AGAATAACGG CATGAATCAG 
GAACAGTACA AGGATCTGGT CCAAAAAGTA GTGGCCATCA ATGAGAATAG CAAAATAAAA 
GAGTCCCGGC GCCGAGACAA CGATCTCGAG CGCAATGCGG CCAGGGATGC AGTGCTACGC 
AAACGTATTC CCAAGCTGAA GGGCAACGAA AATCATTCTG CGGGCTCACC GCATACCGAT 
GGATCGCCCA GGTACGATGA TCAGCCGGCA GCTGCCCCGG AGAAGCCCTC AAACAAACGG 
GATAAGACCA AGAGGGAGAT GAAGCGTCGC AAGCCCACCA AATGGGGCGA ACAAGTGGAT 
GCAGGGGCAG CACAACGCAC TGCCTGGCAA CTGGCCAATG TCAACAATAA TAATAATAAT 
AACAACAACA AGCGGGGCGG TATCCAATTG CCCGTTCAGC CTCAGGCTGG ATTCCGTGGC 
ATGCCCTGGC AGCAGCCACC AGCCATGGTG TTGGGCCAGT CCGCAGTTCC GCCGCCACCG 
CAACCGCCGT CGATGATTAG TATGCCTCCC GTGCCACCGG TGACCATGAC CAAGGCCATA 
AATTCCCTTG ACAATCCAAT GGCTGATGTA GTGCGAAGCA TCACGATCGA CGGTGGCTCC 
AAGGAGATAC GCTTCTACAA CCAGGTGGCC ATCATTTTCA TGGATGGCGA TCAGCCGCAT 
GAAATTGGCT TCCAGCCGGG TCAGCGGGTG ATCTTCATTG ATCACAATGA ACCGCTACCG 
CTTTGCTTCA ACGACGATTA CAAGCCTTTC CACTTGGATG GACAGCTTCA CCGGATTCGG 
TTTGGCTTTC CATCGCGCGA ACTTTATATC GACGAGCACT GGTACGAGAT CTACTTTGGC 
GGTCCAGCCG TATCCGTGCC CATTGGCAAC AAACTGCATA TAATCAAAGC TGAGGGTCCA 
CCGCCCAACG TGGACATTGG TCGAGTGCGA AGGGATCTGG TTGTGGGCAA AATCAATATG 
ATTGCGGATG CGCATACTAT AGTGCCACTC TTTCTGGACG CCAGACAGCA GACCTTCCAG 
CTCGGCGCCG AGCAGCATTC ACTGCAGTTC GTGGACAGTT TCCAATATGC TCTGCTCGAC 
GGCCAGCTGC AGAAGATGGA GTATGGTGGT CTCCCAAAAG GAATGATGCT GAACGGCGGT 
CGCAGCTGCT TCATTAGGTT TGGAACGCTG CCCAAAGGAG TCATTGCTGG AAAAACCCAT 
GTGGCCGATA TGGTGTACAT TAAGACTGAT GCTCCTGCAG AGCCTCCCAA GCCGCCGCCA 
ATTATTGTGA AACCACCGCC AGTGGTGGAG CAACCGAAGC CCGCTCCAGT TGCTGTTGCT 
CCCATTTCCC TTCCAGCAGC AGCCGCCGCA CTTGAGAGCT TAAACATCAA TGATTTGTTT 
CAAAAGCTTG TCTCGTCTGG AATAATTGGT GGTGCAACTG CAGCACCAAC TCTCCCTGCC 
GCAGACTCAT CAGCGGCCAA AGAGCCAACC GCTTCTGCCA CCGAACCCTC AACTGCATCT 
GCAACCGTTG CGCCTGCTAC ACTGCCTGCT GGTCCCCCCG CGGAGCCGAT CAAGCGCATC 
GATCTCCGCA AGCCAGAGTC CATCAAGACA CGCCAGGCGG CTGTGGTGGC CGTTCTTTAC 
CTGGGAATGC AGTGCAGCAG CTGTGGAGTT CGTTTCCCGC CAGAGCAAAC AATTAAGTAC 
AGCCAACATT TGGACTGGCA CTTCCGACAG AATCGTAGAG AGCGGGATTC CACTAGGAAA 
GCCACTTCGC GAAAATGGTA TTACGATCTC AACGACTGGC GGCAATACGA AGAAATCGAA 
GATGTGGAGG AGCGAGAGAA GAACTTCCTC GAGGCTCAGG GACAGCCAGG TGGCGTTGAG 
GCCCTTGATG AACTCTCACA ACAACGCTCG CTGGATTCGC CCGTGCCAAC ATGTGCTGCT 
GGAAGGGATG ATGTGGATCA CTGCTGTGAC ATGTGTCACG AGAAATTCGA GCAGTTCTAC 
AACGAAGAGC TCGAGGAGTG GCATCTGCGT AGCGCTATTC GTGTGGAAGA TAAGATCTAT 
CATCCTCTAT GCTACGAGGA CTACAAGGCC TCGTTGAATC CTCCAACGGA GGTAAAGTCC 
GACCAGGATG TGGACATGAA CAACACCGAT GACAATGCCA TGGACACACT AATCAAAGTG 
GAGGATGATG AAGATGAAGG TACGCCAAAC TCATCACAAC CCATTTTTGA AGACGACGAT 
GACGATGTCA TTGTGCTGCC CAATGAAGAG CCAAGTGTCA CGGAAATCGT CGACGACGAC 
GATGAGGACG AATACGTTCC GGGCAATGTG ACACGTGCGG ACATGGGCAA CGAGTCACAG 
GACAAAACAG AGTCCAACTC CGAGACCAAA GAACCGAAGA AAGATCAGGT TGAGCTCGGT 
GAACAGCAGC AAAACGAATC GGCCAATGAA TCGGATGTCG AAATCCAAGA GCCGAACATT 
CCTTTCACGG ATCTGGACAC TTATGTGGAG AAAGAGATGG ATGAGGCGAC CAGGGCGGCG 
CTACTGAACG TAAAGATCAA GGAGGAGCCC AAGGATGAGT ATGAGGAGGA CGAGGATGAT 
GGATTCGAAG ACGTGGGCAC GGTGGTTTCA CTTTTACCTC TGCCCGAGGA CGAGATCTCC 
ATACACAGCA GCGAAACTCA AACACACACC ATCGGCTCGT CAGCCTCGCC GGCCACCATC 
GAGCGGCCAG CATCGGTGAC CTCGCTCTCC CTGCCCGCCA ATGAGGCGGA CGAGTTGGAG 
CAGGGTGATG CAAACACCGA TACGGATGCG GAATTGAATG GCGAGAAGCA GGAGGCCACG 
CATAACCTGA GCGCAGTGGG TCCGGCGTTA CCTTTGGCTA GCATAGTTAA TAAAATAAAG 
ATAAATATCA CTAAGAATAC GAGTAGTAAT AGTCATAATA GTGCCTCCAA CACCACGACA 
ACGGACTCGC AAGTCTCGGC CATAAGCGTC ATCGGTGGAT CAGGAGCAGC TGGCGGTGCA 
TCCGGAGCAG GAGACCCTGT CCAGCAGGTG AATGCCATTC AAACCATTTC CACCATTCCA 
GTGCTGTGCG GCGGCAATAC GTTCGTGCCC AAGATTGCGA CCAGCACGCC CTCAAACGTA 
ATCAGCTCGA TCTCGGTAAT TGGCAGCACT TACGGCGCAA GCAGCAGCAG CAATGGCAGC 
CGAAGCACTA CTTCCACCGC ATCCTCCGCA CCAGCTGCTT CGCTTTTGGC GGAGACGGGC 
AGCCTGAAAT CGCCAACTCC GCCGCTCGCC ACAGTTGATC CGGATCCGGA GCCCGTCGTG 
GAACAAAAGC CGGCGCTGCG GAATGCCACG CTGAAGCGGA CGAAAAAGGT GCAGAACGGC 
ATCGAAACGT CGGGCCTGTG CTCGATCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286428.1
CDS	234..5786
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKLSMLNSNPTTSGTPAASLVRQSSPSPSSSSISSQGNVSDALQLSINKLLQVQGGAGEKRPADALPPHIDGEEQPPQQKRSKSAKLDALFGSEDVDLRREILVVKPGVIVVEDDSMDECDVDKPTKKSGSPPKKATLEELRAKLANSARIQNKQGKSDKSRDQAVSQRLKQLAELKVNDDSQEAHDEKVRTILSQAQEMYENNGMNQEQYKDLVQKVVAINENSKIKESRRRDNDLERNAARDAVLRKRIPKLKGNENHSAGSPHTDGSPRYDDQPAAAPEKPSNKRDKTKREMKRRKPTKWGEQVDAGAAQRTAWQLANVNNNNNNNNNKRGGIQLPVQPQAGFRGMPWQQPPAMVLGQSAVPPPPQPPSMISMPPVPPVTMTKAINSLDNPMADVVRSITIDGGSKEIRFYNQVAIIFMDGDQPHEIGFQPGQRVIFIDHNEPLPLCFNDDYKPFHLDGQLHRIRFGFPSRELYIDEHWYEIYFGGPAVSVPIGNKLHIIKAEGPPPNVDIGRVRRDLVVGKINMIADAHTIVPLFLDARQQTFQLGAEQHSLQFVDSFQYALLDGQLQKMEYGGLPKGMMLNGGRSCFIRFGTLPKGVIAGKTHVADMVYIKTDAPAEPPKPPPIIVKPPPVVEQPKPAPVAVAPISLPAAAAALESLNINDLFQKLVSSGIIGGATAAPTLPAADSSAAKEPTASATEPSTASATVAPATLPAGPPAEPIKRIDLRKPESIKTRQAAVVAVLYLGMQCSSCGVRFPPEQTIKYSQHLDWHFRQNRRERDSTRKATSRKWYYDLNDWRQYEEIEDVEEREKNFLEAQGQPGGVEALDELSQQRSLDSPVPTCAAGRDDVDHCCDMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCYEDYKASLNPPTEVKSDQDVDMNNTDDNAMDTLIKVEDDEDEGTPNSSQPIFEDDDDDVIVLPNEEPSVTEIVDDDDEDEYVPGNVTRADMGNESQDKTESNSETKEPKKDQVELGEQQQNESANESDVEIQEPNIPFTDLDTYVEKEMDEATRAALLNVKIKEEPKDEYEEDEDDGFEDVGTVVSLLPLPEDEISIHSSETQTHTIGSSASPATIERPASVTSLSLPANEADELEQGDANTDTDAELNGEKQEATHNLSAVGPALPLASIVNKIKINITKNTSSNSHNSASNTTTTDSQVSAISVIGGSGAAGGASGAGDPVQQVNAIQTISTIPVLCGGNTFVPKIATSTPSNVISSISVIGSTYGASSSSNGSRSTTSTASSAPAASLLAETGSLKSPTPPLATVDPDPEPVVEQKPALRNATLKRTKKVQNGIETSGLCSIM

Target ORF information:

RefSeq Version	NM_001299499.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant F (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299499.1

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACTATCAAT GCTCAACTCA 
AATCCCACGA CTTCAGGAAC ACCAGCGGCA TCTCTAGTCA GGCAATCGTC GCCCAGTCCT 
TCGTCCTCGT CTATTTCCTC GCAGGGTAAT GTATCGGACG CCCTGCAGCT GTCGATTAAC 
AAGTTGCTCC AGGTGCAAGG CGGTGCCGGC GAGAAACGTC CCGCCGACGC ACTGCCGCCA 
CATATCGACG GTGAAGAGCA ACCGCCGCAG CAAAAGCGCA GCAAGTCAGC CAAACTGGAC 
GCTCTCTTTG GCAGTGAGGA TGTCGACCTG CGACGCGAGA TTCTAGTTGT CAAGCCAGGA 
GTTATTGTTG TCGAAGACGA CTCTATGGAC GAATGCGATG TAGACAAGCC CACAAAGAAA 
TCTGGGTCGC CGCCCAAGAA GGCAACGTTG GAGGAGCTGC GCGCCAAGTT GGCTAACTCG 
GCTCGAATCC AGAATAAGCA GGGAAAGTCT GACAAATCCA GGGATCAGGC CGTGTCACAA 
CGCCTTAAGC AGCTGGCGGA GCTTAAAGTC AACGACGATT CGCAGGAGGC GCACGACGAG 
AAGGTGCGCA CCATTCTCAG CCAGGCGCAG GAGATGTATG AGAATAACGG CATGAATCAG 
GAACAGTACA AGGATCTGGT CCAAAAAGTA GTGGCCATCA ATGAGAATAG CAAAATAAAA 
GAGTCCCGGC GCCGAGACAA CGATCTCGAG CGCAATGCGG CCAGGGATGC AGTGCTACGC 
AAACGTATTC CCAAGCTGAA GGGCAACGAA AATCATTCTG CGGGCTCACC GCATACCGAT 
GGATCGCCCA GGTACGATGA TCAGCCGGCA GCTGCCCCGG AGAAGCCCTC AAACAAACGG 
GATAAGACCA AGAGGGAGAT GAAGCGTCGC AAGCCCACCA AATGGGGCGA ACAAGTGGAT 
GCAGGGGCAG CACAACGCAC TGCCTGGCAA CTGGCCAATG TCAACAATAA TAATAATAAT 
AACAACAACA AGCGGGGCGG TATCCAATTG CCCGTTCAGC CTCAGGCTGG ATTCCGTGGC 
ATGCCCTGGC AGCAGCCACC AGCCATGGTG TTGGGCCAGT CCGCAGTTCC GCCGCCACCG 
CAACCGCCGT CGATGATTAG TATGCCTCCC GTGCCACCGG TGACCATGAC CAAGGCCATA 
AATTCCCTTG ACAATCCAAT GGCTGATGTA GTGCGAAGCA TCACGATCGA CGGTGGCTCC 
AAGGAGATAC GCTTCTACAA CCAGGTGGCC ATCATTTTCA TGGATGGCGA TCAGCCGCAT 
GAAATTGGCT TCCAGCCGGG TCAGCGGGTG ATCTTCATTG ATCACAATGA ACCGCTACCG 
CTTTGCTTCA ACGACGATTA CAAGCCTTTC CACTTGGATG GACAGCTTCA CCGGATTCGG 
TTTGGCTTTC CATCGCGCGA ACTTTATATC GACGAGCACT GGTACGAGAT CTACTTTGGC 
GGTCCAGCCG TATCCGTGCC CATTGGCAAC AAACTGCATA TAATCAAAGC TGAGGGTCCA 
CCGCCCAACG TGGACATTGG TCGAGTGCGA AGGGATCTGG TTGTGGGCAA AATCAATATG 
ATTGCGGATG CGCATACTAT AGTGCCACTC TTTCTGGACG CCAGACAGCA GACCTTCCAG 
CTCGGCGCCG AGCAGCATTC ACTGCAGTTC GTGGACAGTT TCCAATATGC TCTGCTCGAC 
GGCCAGCTGC AGAAGATGGA GTATGGTGGT CTCCCAAAAG GAATGATGCT GAACGGCGGT 
CGCAGCTGCT TCATTAGGTT TGGAACGCTG CCCAAAGGAG TCATTGCTGG AAAAACCCAT 
GTGGCCGATA TGGTGTACAT TAAGACTGAT GCTCCTGCAG AGCCTCCCAA GCCGCCGCCA 
ATTATTGTGA AACCACCGCC AGTGGTGGAG CAACCGAAGC CCGCTCCAGT TGCTGTTGCT 
CCCATTTCCC TTCCAGCAGC AGCCGCCGCA CTTGAGAGCT TAAACATCAA TGATTTGTTT 
CAAAAGCTTG TCTCGTCTGG AATAATTGGT GGTGCAACTG CAGCACCAAC TCTCCCTGCC 
GCAGACTCAT CAGCGGCCAA AGAGCCAACC GCTTCTGCCA CCGAACCCTC AACTGCATCT 
GCAACCGTTG CGCCTGCTAC ACTGCCTGCT GGTCCCCCCG CGGAGCCGAT CAAGCGCATC 
GATCTCCGCA AGCCAGAGTC CATCAAGACA CGCCAGGCGG CTGTGGTGGC CGTTCTTTAC 
CTGGGAATGC AGTGCAGCAG CTGTGGAGTT CGTTTCCCGC CAGAGCAAAC AATTAAGTAC 
AGCCAACATT TGGACTGGCA CTTCCGACAG AATCGTAGAG AGCGGGATTC CACTAGGAAA 
GCCACTTCGC GAAAATGGTA TTACGATCTC AACGACTGGC GGCAATACGA AGAAATCGAA 
GATGTGGAGG AGCGAGAGAA GAACTTCCTC GAGGCTCAGG GACAGCCAGG TGGCGTTGAG 
GCCCTTGATG AACTCTCACA ACAACGCTCG CTGGATTCGC CCGTGCCAAC ATGTGCTGCT 
GGAAGGGATG ATGTGGATCA CTGCTGTGAC ATGTGTCACG AGAAATTCGA GCAGTTCTAC 
AACGAAGAGC TCGAGGAGTG GCATCTGCGT AGCGCTATTC GTGTGGAAGA TAAGATCTAT 
CATCCTCTAT GCTACGAGGA CTACAAGGCC TCGTTGAATC CTCCAACGGA GGTAAAGTCC 
GACCAGGATG TGGACATGAA CAACACCGAT GACAATGCCA TGGACACACT AATCAAAGTG 
GAGGATGATG AAGATGAAGG TACGCCAAAC TCATCACAAC CCATTTTTGA AGACGACGAT 
GACGATGTCA TTGTGCTGCC CAATGAAGAG CCAAGTGTCA CGGAAATCGT CGACGACGAC 
GATGAGGACG AATACGTTCC GGGCAATGTG ACACGTGCGG ACATGGGCAA CGAGTCACAG 
GACAAAACAG AGTCCAACTC CGAGACCAAA GAACCGAAGA AAGATCAGGT TGAGCTCGGT 
GAACAGCAGC AAAACGAATC GGCCAATGAA TCGGATGTCG AAATCCAAGA GCCGAACATT 
CCTTTCACGG ATCTGGACAC TTATGTGGAG AAAGAGATGG ATGAGGCGAC CAGGGCGGCG 
CTACTGAACG TAAAGATCAA GGAGGAGCCC AAGGATGAGT ATGAGGAGGA CGAGGATGAT 
GGATTCGAAG ACGTGGGCAC GGTGGTTTCA CTTTTACCTC TGCCCGAGGA CGAGATCTCC 
ATACACAGCA GCGAAACTCA AACACACACC ATCGGCTCGT CAGCCTCGCC GGCCACCATC 
GAGCGGCCAG CATCGGTGAC CTCGCTCTCC CTGCCCGCCA ATGAGGCGGA CGAGTTGGAG 
CAGGGTGATG CAAACACCGA TACGGATGCG GAATTGAATG GCGAGAAGCA GGAGGCCACG 
CATAACCTGA GCGCAGTGGG TCCGGCGTTA CCTTTGGCTA GCATAGTTAA TAAAATAAAG 
ATAAATATCA CTAAGAATAC GAGTAGTAAT AGTCATAATA GTGCCTCCAA CACCACGACA 
ACGGACTCGC AAGTCTCGGC CATAAGCGTC ATCGGTGGAT CAGGAGCAGC TGGCGGTGCA 
TCCGGAGCAG GAGACCCTGT CCAGCAGGTG AATGCCATTC AAACCATTTC CACCATTCCA 
GTGCTGTGCG GCGGCAATAC GTTCGTGCCC AAGATTGCGA CCAGCACGCC CTCAAACGTA 
ATCAGCTCGA TCTCGGTAAT TGGCAGCACT TACGGCGCAA GCAGCAGCAG CAATGGCAGC 
CGAAGCACTA CTTCCACCGC ATCCTCCGCA CCAGCTGCTT CGCTTTTGGC GGAGACGGGC 
AGCCTGAAAT CGCCAACTCC GCCGCTCGCC ACAGTTGATC CGGATCCGGA GCCCGTCGTG 
GAACAAAAGC CGGCGCTGCG GAATGCCACG CTGAAGCGGA CGAAAAAGGT GCAGAACGGC 
ATCGAAACGT CGGGCCTGTG CTCGATCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcf11 ( NM_001299500.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286429.1 Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant G (Pcf11), mRNA.

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CloneID	OFa21810
Clone ID Related Accession (Same CDS sequence)	NM_001299500.1
Accession Version	NM_001299500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein 1 of cleavage and polyadenylation factor 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGCCATCC TCCAGCTCCT 
CCAAGTCCGC CGATTATAGA TGTATCGAGT ATGGACATTG ACTTGAGGCG GCCAAAGACG 
CCGCCTCCTC CGACATTCGA CTCAGTGGAT AGCCTCGACC AGAAGGATAC AACTAGCGGT 
CACATCAGCA GCACAATAAT ACAAGCTAGT GGCGCCTCCA ACGCCTCCCC ATCAGGCGCG 
TCCACTTCCA AATTGCCCGC AAAAGTGTCG TTCAAAATAC AAAAACAGTT CAATAAATTA 
GAAAAATCTA CAGCGAATCT ATCAACAGTA TCAATGCTCA ACTCAAATCC CACGACTTCA 
GGAACACCAG CGGCATCTCT AGTCAGGCAA TCGTCGCCCA GTCCTTCGTC CTCGTCTATT 
TCCTCGCAGG GTAATGTATC GGACGCCCTG CAGCTGTCGA TTAACAAGTT GCTCCAGGTG 
CAAGGCGGTG CCGGCGAGAA ACGTCCCGCC GACGCACTGC CGCCACATAT CGACGGTGAA 
GAGCAACCGC CGCAGCAAAA GCGCAGCAAG TCAGCCAAAC TGGACGCTCT CTTTGGCAGT 
GAGGATGTCG ACCTGCGACG CGAGATTCTA GTTGTCAAGC CAGGAGTTAT TGTTGTCGAA 
GACGACTCTA TGGACGAATG CGATGTAGAC AAGCCCACAA AGAAATCTGG GTCGCCGCCC 
AAGAAGGCAA CGTTGGAGGA GCTGCGCGCC AAGTTGGCTA ACTCGGCTCG AATCCAGAAT 
AAGCAGGGAA AGTCTGACAA ATCCAGGGAT CAGGCCGTGT CACAACGCCT TAAGCAGCTG 
GCGGAGCTTA AAGTCAACGA CGATTCGCAG GAGGCGCACG ACGAGAAGGT GCGCACCATT 
CTCAGCCAGG CGCAGGAGAT GTATGAGAAT AACGGCATGA ATCAGGAACA GTACAAGGAT 
CTGGTCCAAA AAGTAGTGGC CATCAATGAG AATAGCAAAA TAAAAGAGTC CCGGCGCCGA 
GACAACGATC TCGAGCGCAA TGCGGCCAGG GATGCAGTGC TACGCAAACG TATTCCCAAG 
CTGAAGGGCA ACGAAAATCA TTCTGCGGGC TCACCGCATA CCGATGGATC GCCCAGGTAC 
GATGATCAGC CGGCAGCTGC CCCGGAGAAG CCCTCAAACA AACGGGATAA GACCAAGAGG 
GAGATGAAGC GTCGCAAGCC CACCAAATGG GGCGAACAAG TGGATGCAGG GGCAGCACAA 
CGCACTGCCT GGCAACTGGC CAATGTCAAC AATAATAATA ATAATAACAA CAACAAGCGG 
GGCGGTATCC AATTGCCCGT TCAGCCTCAG GCTGGATTCC GTGGCATGCC CTGGCAGCAG 
CCACCAGCCA TGGTGTTGGG CCAGTCCGCA GTTCCGCCGC CACCGCAACC GCCGTCGATG 
ATTAGTATGC CTCCCGTGCC ACCGGTGACC ATGACCAAGG CCATAAATTC CCTTGACAAT 
CCAATGGCTG ATGTAGTGCG AAGCATCACG ATCGACGGTG GCTCCAAGGA GATACGCTTC 
TACAACCAGG TGGCCATCAT TTTCATGGAT GGCGATCAGC CGCATGAAAT TGGCTTCCAG 
CCGGGTCAGC GGGTGATCTT CATTGATCAC AATGAACCGC TACCGCTTTG CTTCAACGAC 
GATTACAAGC CTTTCCACTT GGATGGACAG CTTCACCGGA TTCGGTTTGG CTTTCCATCG 
CGCGAACTTT ATATCGACGA GCACTGGTAC GAGATCTACT TTGGCGGTCC AGCCGTATCC 
GTGCCCATTG GCAACAAACT GCATATAATC AAAGCTGAGG GTCCACCGCC CAACGTGGAC 
ATTGGTCGAG TGCGAAGGGA TCTGGTTGTG GGCAAAATCA ATATGATTGC GGATGCGCAT 
ACTATAGTGC CACTCTTTCT GGACGCCAGA CAGCAGACCT TCCAGCTCGG CGCCGAGCAG 
CATTCACTGC AGTTCGTGGA CAGTTTCCAA TATGCTCTGC TCGACGGCCA GCTGCAGAAG 
ATGGAGTATG GTGGTCTCCC AAAAGGAATG ATGCTGAACG GCGGTCGCAG CTGCTTCATT 
AGGTTTGGAA CGCTGCCCAA AGGAGTCATT GCTGGAAAAA CCCATGTGGC CGATATGGTG 
TACATTAAGA CTGATGCTCC TGCAGAGCCT CCCAAGCCGC CGCCAATTAT TGTGAAACCA 
CCGCCAGTGG TGGAGCAACC GAAGCCCGCT CCAGTTGCTG TTGCTCCCAT TTCCCTTCCA 
GCAGCAGCCG CCGCACTTGA GAGCTTAAAC ATCAATGATT TGTTTCAAAA GCTTGTCTCG 
TCTGGAATAA TTGGTGGTGC AACTGCAGCA CCAACTCTCC CTGCCGCAGA CTCATCAGCG 
GCCAAAGAGC CAACCGCTTC TGCCACCGAA CCCTCAACTG CATCTGCAAC CGTTGCGCCT 
GCTACACTGC CTGCTGGTCC CCCCGCGGAG CCGATCAAGC GCATCGATCT CCGCAAGCCA 
GAGTCCATCA AGACACGCCA GGCGGCTGTG GTGGCCGTTC TTTACCTGGG AATGCAGTGC 
AGCAGCTGTG GAGTTCGTTT CCCGCCAGAG CAAACAATTA AGTACAGCCA ACATTTGGAC 
TGGCACTTCC GACAGAATCG TAGAGAGCGG GATTCCACTA GGAAAGCCAC TTCGCGAAAA 
TGGTATTACG ATCTCAACGA CTGGCGGCAA TACGAAGAAA TCGAAGATGT GGAGGAGCGA 
GAGAAGAACT TCCTCGAGGC TCAGGGACAG CCAGGTGGCG TTGAGGCCCT TGATGAACTC 
TCACAACAAC GCTCGCTGGA TTCGCCCGTG CCAACATGTG CTGCTGGAAG GGATGATGTG 
GATCACTGCT GTGACATGTG TCACGAGAAA TTCGAGCAGT TCTACAACGA AGAGCTCGAG 
GAGTGGCATC TGCGTAGCGC TATTCGTGTG GAAGATAAGA TCTATCATCC TCTATGCTAC 
GAGGACTACA AGGCCTCGTT GAATCCTCCA ACGGAGGTAA AGTCCGACCA GGATGTGGAC 
ATGAACAACA CCGATGACAA TGCCATGGAC ACACTAATCA AAGTGGAGGA TGATGAAGAT 
GAAGGTACGC CAAACTCATC ACAACCCATT TTTGAAGACG ACGATGACGA TGTCATTGTG 
CTGCCCAATG AAGAGCCAAG TGTCACGGAA ATCGTCGACG ACGACGATGA GGACGAATAC 
GTTCCGGGCA ATGTGACACG TGCGGACATG GGCAACGAGT CACAGGACAA AACAGAGTCC 
AACTCCGAGA CCAAAGAACC GAAGAAAGAT CAGGTTGAGC TCGGTGAACA GCAGCAAAAC 
GAATCGGCCA ATGAATCGGA TGTCGAAATC CAAGAGCCGA ACATTCCTTT CACGGATCTG 
GACACTTATG TGGAGAAAGA GATGGATGAG GCGACCAGGG CGGCGCTACT GAACGTAAAG 
ATCAAGGAGG AGCCCAAGGA TGAGTATGAG GAGGACGAGG ATGATGGATT CGAAGACGTG 
GGCACGGTGG TTTCACTTTT ACCTCTGCCC GAGGACGAGA TCTCCATACA CAGCAGCGAA 
ACTCAAACAC ACACCATCGG CTCGTCAGCC TCGCCGGCCA CCATCGAGCG GCCAGCATCG 
GTGACCTCGC TCTCCCTGCC CGCCAATGAG GCGGACGAGT TGGAGCAGGG TGATGCAAAC 
ACCGATACGG ATGCGGAATT GAATGGCGAG AAGCAGGAGG CCACGCATAA CCTGAGCGCA 
GTGGGTCCGG CGTTACCTTT GGCTAGCATA GTTAATAAAA TAAAGATAAA TATCACTAAG 
AATACGAGTA GTAATAGTCA TAATAGTGCC TCCAACACCA CGACAACGGA CTCGCAAGTC 
TCGGCCATAA GCGTCATCGG TGGATCAGGA GCAGCTGGCG GTGCATCCGG AGCAGGAGAC 
CCTGTCCAGC AGGTGAATGC CATTCAAACC ATTTCCACCA TTCCAGTGCT GTGCGGCGGC 
AATACGTTCG TGCCCAAGAT TGCGACCAGC ACGCCCTCAA ACGTAATCAG CTCGATCTCG 
GTAATTGGCA GCACTTACGG CGCAAGCAGC AGCAGCAATG GCAGCCGAAG CACTACTTCC 
ACCGCATCCT CCGCACCAGC TGCTTCGCTT TTGGCGGAGA CGGGCAGCCT GAAATCGCCA 
ACTCCGCCGC TCGCCACAGT TGATCCGGAT CCGGAGCCCG TCGTGGAACA AAAGCCGGCG 
CTGCGGAATG CCACGCTGAA GCGGACGAAA AAGGTGCAGA ACGGCATCGA AACGTCGGGC 
CTGTGCTCGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286429.1
CDS	234..6071
Translation	MEQLFQNYRDDERRIGEEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDSIVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMYALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEILQAKTRELLELKKRKLELELEQTKKHLEEQERQLTQTTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQRAPGVMGNVGPVMHNMPVAPQLFANKPKVHPVNPALLNSVRQRDPRLARQMHAASSHPAAPARNDPRLEAKSSSSQKSSRSRSKSPVRNSSSRSGKSGSSSHSSSSSRKRSESKSSTTSSSSSSSDVRHKGVTASSSNQPTVKRSGKITSKDSDRYVRNGSPLGSAKRKSSSPSSSPSKSKRSTSHKSSSSRGKTSSLRSRSRSPVFMDVDLRTGVRSKSPESRAVAPSSLPLAGASLPASAKAPKDLEKRHPPAPPSPPIIDVSSMDIDLRRPKTPPPPTFDSVDSLDQKDTTSGHISSTIIQASGASNASPSGASTSKLPAKVSFKIQKQFNKLEKSTANLSTVSMLNSNPTTSGTPAASLVRQSSPSPSSSSISSQGNVSDALQLSINKLLQVQGGAGEKRPADALPPHIDGEEQPPQQKRSKSAKLDALFGSEDVDLRREILVVKPGVIVVEDDSMDECDVDKPTKKSGSPPKKATLEELRAKLANSARIQNKQGKSDKSRDQAVSQRLKQLAELKVNDDSQEAHDEKVRTILSQAQEMYENNGMNQEQYKDLVQKVVAINENSKIKESRRRDNDLERNAARDAVLRKRIPKLKGNENHSAGSPHTDGSPRYDDQPAAAPEKPSNKRDKTKREMKRRKPTKWGEQVDAGAAQRTAWQLANVNNNNNNNNNKRGGIQLPVQPQAGFRGMPWQQPPAMVLGQSAVPPPPQPPSMISMPPVPPVTMTKAINSLDNPMADVVRSITIDGGSKEIRFYNQVAIIFMDGDQPHEIGFQPGQRVIFIDHNEPLPLCFNDDYKPFHLDGQLHRIRFGFPSRELYIDEHWYEIYFGGPAVSVPIGNKLHIIKAEGPPPNVDIGRVRRDLVVGKINMIADAHTIVPLFLDARQQTFQLGAEQHSLQFVDSFQYALLDGQLQKMEYGGLPKGMMLNGGRSCFIRFGTLPKGVIAGKTHVADMVYIKTDAPAEPPKPPPIIVKPPPVVEQPKPAPVAVAPISLPAAAAALESLNINDLFQKLVSSGIIGGATAAPTLPAADSSAAKEPTASATEPSTASATVAPATLPAGPPAEPIKRIDLRKPESIKTRQAAVVAVLYLGMQCSSCGVRFPPEQTIKYSQHLDWHFRQNRRERDSTRKATSRKWYYDLNDWRQYEEIEDVEEREKNFLEAQGQPGGVEALDELSQQRSLDSPVPTCAAGRDDVDHCCDMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCYEDYKASLNPPTEVKSDQDVDMNNTDDNAMDTLIKVEDDEDEGTPNSSQPIFEDDDDDVIVLPNEEPSVTEIVDDDDEDEYVPGNVTRADMGNESQDKTESNSETKEPKKDQVELGEQQQNESANESDVEIQEPNIPFTDLDTYVEKEMDEATRAALLNVKIKEEPKDEYEEDEDDGFEDVGTVVSLLPLPEDEISIHSSETQTHTIGSSASPATIERPASVTSLSLPANEADELEQGDANTDTDAELNGEKQEATHNLSAVGPALPLASIVNKIKINITKNTSSNSHNSASNTTTTDSQVSAISVIGGSGAAGGASGAGDPVQQVNAIQTISTIPVLCGGNTFVPKIATSTPSNVISSISVIGSTYGASSSSNGSRSTTSTASSAPAASLLAETGSLKSPTPPLATVDPDPEPVVEQKPALRNATLKRTKKVQNGIETSGLCSIM

Target ORF information:

RefSeq Version	NM_001299500.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein 1 of cleavage and polyadenylation factor 1, transcript variant G (Pcf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299500.1

ORF Insert Sequence:

ATGGAGCAGC TATTTCAGAA TTACCGCGAC GATGAGCGAA GGATCGGCGA GGAGTATCTG 
TCAAGTCTCC AGGACCTCAA CTGCAACAGC AAGCCATTGA TCAATATGCT CACGATGCTT 
GCCGAGGAGA ACATCAACTA CGCCCACATC ATAGTTAAAG TGGTGGAATA TTACATCAGC 
CAGGTGGCGC CCGAATTTAA ATTGCCCATA CTATATTTAA TTGATTCGAT AGTAAAGAAT 
GTGAAAAGCA GCTACGTCCA ATTGTTTGGA CAGTGCATAG TCAACATATT CCTCCACGCC 
TTCGAATCGG TCCAGCACTC ACAGTCGCAG GTTTTGGAAA AGGTGCGGGA ACGCATGTAT 
GCCCTGCGTC AGACCTGGAA CGAGGTGTTC CCGCCATCCA AGATGTACGC GCTGGACGTT 
AAGGTGAAGC GCCTAGATAA CAACTGGCCC ATTACTGCCA AACAGCCAAC TAACAAAATT 
CACGTCAATC CGGCCATTCA TGTTAATCCG GATTTCTTAA AACCGGGTTT GGTTCCTGGG 
ATGCCTGGTA ACCCCACAAT CACCAGCGAC ATGGAGGAGA TTTTGCAAGC AAAAACTCGT 
GAGTTGCTGG AGCTCAAGAA GCGCAAACTG GAACTGGAGC TGGAGCAAAC CAAAAAGCAT 
TTAGAGGAGC AGGAGCGTCA GCTGACCCAA ACCACGGACG CGATGGTTGG AGCACCGATT 
ATTATGCCTG CTCCCACTCC AGCAGCAATT TGTCCGCCAA TCACGGATCC CGGTATAGCA 
GTGCATAATC AACGAGCTCC AGGAGTAATG GGCAATGTTG GTCCCGTAAT GCATAACATG 
CCAGTGGCTC CGCAGCTTTT TGCGAATAAA CCAAAAGTCC ATCCTGTCAA TCCGGCACTG 
TTGAACTCGG TGCGCCAACG AGATCCGAGA CTAGCCCGCC AGATGCATGC GGCATCCTCA 
CATCCAGCTG CGCCGGCACG AAACGATCCT CGGCTGGAGG CCAAATCTAG CTCCTCACAG 
AAGTCTAGTC GGTCGCGCAG CAAATCGCCT GTGCGCAACA GCAGCAGTCG GTCTGGAAAG 
AGTGGAAGCT CTAGCCACAG CAGTTCGTCG AGCCGCAAGC GAAGTGAGTC AAAGAGCTCC 
ACGACTTCCT CATCTTCGTC GAGCTCTGAT GTTCGGCACA AGGGTGTCAC TGCAAGCAGC 
TCCAACCAGC CAACAGTTAA GCGGTCCGGT AAGATCACGT CTAAGGATTC GGATCGCTAT 
GTTCGCAATG GATCTCCTTT GGGATCCGCG AAACGCAAGA GCAGCTCACC AAGTAGCTCG 
CCCTCCAAAT CCAAGCGCAG CACGTCGCAC AAGTCATCCT CGTCGCGAGG AAAGACCTCT 
TCTCTGCGTT CCCGCAGTCG ATCTCCAGTT TTTATGGACG TCGACCTACG CACCGGAGTT 
CGAAGCAAGT CACCAGAGAG CAGGGCAGTT GCCCCATCAT CCTTACCACT GGCGGGCGCA 
TCACTACCAG CATCAGCTAA AGCACCAAAA GATTTAGAGA AACGCCATCC TCCAGCTCCT 
CCAAGTCCGC CGATTATAGA TGTATCGAGT ATGGACATTG ACTTGAGGCG GCCAAAGACG 
CCGCCTCCTC CGACATTCGA CTCAGTGGAT AGCCTCGACC AGAAGGATAC AACTAGCGGT 
CACATCAGCA GCACAATAAT ACAAGCTAGT GGCGCCTCCA ACGCCTCCCC ATCAGGCGCG 
TCCACTTCCA AATTGCCCGC AAAAGTGTCG TTCAAAATAC AAAAACAGTT CAATAAATTA 
GAAAAATCTA CAGCGAATCT ATCAACAGTA TCAATGCTCA ACTCAAATCC CACGACTTCA 
GGAACACCAG CGGCATCTCT AGTCAGGCAA TCGTCGCCCA GTCCTTCGTC CTCGTCTATT 
TCCTCGCAGG GTAATGTATC GGACGCCCTG CAGCTGTCGA TTAACAAGTT GCTCCAGGTG 
CAAGGCGGTG CCGGCGAGAA ACGTCCCGCC GACGCACTGC CGCCACATAT CGACGGTGAA 
GAGCAACCGC CGCAGCAAAA GCGCAGCAAG TCAGCCAAAC TGGACGCTCT CTTTGGCAGT 
GAGGATGTCG ACCTGCGACG CGAGATTCTA GTTGTCAAGC CAGGAGTTAT TGTTGTCGAA 
GACGACTCTA TGGACGAATG CGATGTAGAC AAGCCCACAA AGAAATCTGG GTCGCCGCCC 
AAGAAGGCAA CGTTGGAGGA GCTGCGCGCC AAGTTGGCTA ACTCGGCTCG AATCCAGAAT 
AAGCAGGGAA AGTCTGACAA ATCCAGGGAT CAGGCCGTGT CACAACGCCT TAAGCAGCTG 
GCGGAGCTTA AAGTCAACGA CGATTCGCAG GAGGCGCACG ACGAGAAGGT GCGCACCATT 
CTCAGCCAGG CGCAGGAGAT GTATGAGAAT AACGGCATGA ATCAGGAACA GTACAAGGAT 
CTGGTCCAAA AAGTAGTGGC CATCAATGAG AATAGCAAAA TAAAAGAGTC CCGGCGCCGA 
GACAACGATC TCGAGCGCAA TGCGGCCAGG GATGCAGTGC TACGCAAACG TATTCCCAAG 
CTGAAGGGCA ACGAAAATCA TTCTGCGGGC TCACCGCATA CCGATGGATC GCCCAGGTAC 
GATGATCAGC CGGCAGCTGC CCCGGAGAAG CCCTCAAACA AACGGGATAA GACCAAGAGG 
GAGATGAAGC GTCGCAAGCC CACCAAATGG GGCGAACAAG TGGATGCAGG GGCAGCACAA 
CGCACTGCCT GGCAACTGGC CAATGTCAAC AATAATAATA ATAATAACAA CAACAAGCGG 
GGCGGTATCC AATTGCCCGT TCAGCCTCAG GCTGGATTCC GTGGCATGCC CTGGCAGCAG 
CCACCAGCCA TGGTGTTGGG CCAGTCCGCA GTTCCGCCGC CACCGCAACC GCCGTCGATG 
ATTAGTATGC CTCCCGTGCC ACCGGTGACC ATGACCAAGG CCATAAATTC CCTTGACAAT 
CCAATGGCTG ATGTAGTGCG AAGCATCACG ATCGACGGTG GCTCCAAGGA GATACGCTTC 
TACAACCAGG TGGCCATCAT TTTCATGGAT GGCGATCAGC CGCATGAAAT TGGCTTCCAG 
CCGGGTCAGC GGGTGATCTT CATTGATCAC AATGAACCGC TACCGCTTTG CTTCAACGAC 
GATTACAAGC CTTTCCACTT GGATGGACAG CTTCACCGGA TTCGGTTTGG CTTTCCATCG 
CGCGAACTTT ATATCGACGA GCACTGGTAC GAGATCTACT TTGGCGGTCC AGCCGTATCC 
GTGCCCATTG GCAACAAACT GCATATAATC AAAGCTGAGG GTCCACCGCC CAACGTGGAC 
ATTGGTCGAG TGCGAAGGGA TCTGGTTGTG GGCAAAATCA ATATGATTGC GGATGCGCAT 
ACTATAGTGC CACTCTTTCT GGACGCCAGA CAGCAGACCT TCCAGCTCGG CGCCGAGCAG 
CATTCACTGC AGTTCGTGGA CAGTTTCCAA TATGCTCTGC TCGACGGCCA GCTGCAGAAG 
ATGGAGTATG GTGGTCTCCC AAAAGGAATG ATGCTGAACG GCGGTCGCAG CTGCTTCATT 
AGGTTTGGAA CGCTGCCCAA AGGAGTCATT GCTGGAAAAA CCCATGTGGC CGATATGGTG 
TACATTAAGA CTGATGCTCC TGCAGAGCCT CCCAAGCCGC CGCCAATTAT TGTGAAACCA 
CCGCCAGTGG TGGAGCAACC GAAGCCCGCT CCAGTTGCTG TTGCTCCCAT TTCCCTTCCA 
GCAGCAGCCG CCGCACTTGA GAGCTTAAAC ATCAATGATT TGTTTCAAAA GCTTGTCTCG 
TCTGGAATAA TTGGTGGTGC AACTGCAGCA CCAACTCTCC CTGCCGCAGA CTCATCAGCG 
GCCAAAGAGC CAACCGCTTC TGCCACCGAA CCCTCAACTG CATCTGCAAC CGTTGCGCCT 
GCTACACTGC CTGCTGGTCC CCCCGCGGAG CCGATCAAGC GCATCGATCT CCGCAAGCCA 
GAGTCCATCA AGACACGCCA GGCGGCTGTG GTGGCCGTTC TTTACCTGGG AATGCAGTGC 
AGCAGCTGTG GAGTTCGTTT CCCGCCAGAG CAAACAATTA AGTACAGCCA ACATTTGGAC 
TGGCACTTCC GACAGAATCG TAGAGAGCGG GATTCCACTA GGAAAGCCAC TTCGCGAAAA 
TGGTATTACG ATCTCAACGA CTGGCGGCAA TACGAAGAAA TCGAAGATGT GGAGGAGCGA 
GAGAAGAACT TCCTCGAGGC TCAGGGACAG CCAGGTGGCG TTGAGGCCCT TGATGAACTC 
TCACAACAAC GCTCGCTGGA TTCGCCCGTG CCAACATGTG CTGCTGGAAG GGATGATGTG 
GATCACTGCT GTGACATGTG TCACGAGAAA TTCGAGCAGT TCTACAACGA AGAGCTCGAG 
GAGTGGCATC TGCGTAGCGC TATTCGTGTG GAAGATAAGA TCTATCATCC TCTATGCTAC 
GAGGACTACA AGGCCTCGTT GAATCCTCCA ACGGAGGTAA AGTCCGACCA GGATGTGGAC 
ATGAACAACA CCGATGACAA TGCCATGGAC ACACTAATCA AAGTGGAGGA TGATGAAGAT 
GAAGGTACGC CAAACTCATC ACAACCCATT TTTGAAGACG ACGATGACGA TGTCATTGTG 
CTGCCCAATG AAGAGCCAAG TGTCACGGAA ATCGTCGACG ACGACGATGA GGACGAATAC 
GTTCCGGGCA ATGTGACACG TGCGGACATG GGCAACGAGT CACAGGACAA AACAGAGTCC 
AACTCCGAGA CCAAAGAACC GAAGAAAGAT CAGGTTGAGC TCGGTGAACA GCAGCAAAAC 
GAATCGGCCA ATGAATCGGA TGTCGAAATC CAAGAGCCGA ACATTCCTTT CACGGATCTG 
GACACTTATG TGGAGAAAGA GATGGATGAG GCGACCAGGG CGGCGCTACT GAACGTAAAG 
ATCAAGGAGG AGCCCAAGGA TGAGTATGAG GAGGACGAGG ATGATGGATT CGAAGACGTG 
GGCACGGTGG TTTCACTTTT ACCTCTGCCC GAGGACGAGA TCTCCATACA CAGCAGCGAA 
ACTCAAACAC ACACCATCGG CTCGTCAGCC TCGCCGGCCA CCATCGAGCG GCCAGCATCG 
GTGACCTCGC TCTCCCTGCC CGCCAATGAG GCGGACGAGT TGGAGCAGGG TGATGCAAAC 
ACCGATACGG ATGCGGAATT GAATGGCGAG AAGCAGGAGG CCACGCATAA CCTGAGCGCA 
GTGGGTCCGG CGTTACCTTT GGCTAGCATA GTTAATAAAA TAAAGATAAA TATCACTAAG 
AATACGAGTA GTAATAGTCA TAATAGTGCC TCCAACACCA CGACAACGGA CTCGCAAGTC 
TCGGCCATAA GCGTCATCGG TGGATCAGGA GCAGCTGGCG GTGCATCCGG AGCAGGAGAC 
CCTGTCCAGC AGGTGAATGC CATTCAAACC ATTTCCACCA TTCCAGTGCT GTGCGGCGGC 
AATACGTTCG TGCCCAAGAT TGCGACCAGC ACGCCCTCAA ACGTAATCAG CTCGATCTCG 
GTAATTGGCA GCACTTACGG CGCAAGCAGC AGCAGCAATG GCAGCCGAAG CACTACTTCC 
ACCGCATCCT CCGCACCAGC TGCTTCGCTT TTGGCGGAGA CGGGCAGCCT GAAATCGCCA 
ACTCCGCCGC TCGCCACAGT TGATCCGGAT CCGGAGCCCG TCGTGGAACA AAAGCCGGCG 
CTGCGGAATG CCACGCTGAA GCGGACGAAA AAGGTGCAGA ACGGCATCGA AACGTCGGGC 
CTGTGCTCGA TCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pcf11 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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