CG8485 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8485
Entrez Gene ID	36579
Full Name	CG8485 gene product from transcript CG8485-RA
Synonyms	152964_at,Dmel\CG8485,SNRK
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50E4-50E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_166042.2	NP_725371.1	uncharacterized protein, isoform C
NM_137098.3	NP_610942.2	uncharacterized protein, isoform A
NM_166041.2	NP_725370.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG8485	NP_610942.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001752	XP_321330.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8485 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8485 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09949	NM_137098.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8485), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09949	NM_166042.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8485), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09949	NM_166041.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8485), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8485 ( NM_166042.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725371.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG8485), mRNA.

Price & Availability↑

CloneID	OFa09949
Clone ID Related Accession (Same CDS sequence)	NM_166042.2 , NM_137098.3 , NM_166041.2
Accession Version	NM_166042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166042.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725371.1
CDS	300..2882
Translation	MTLETQPVGGVSDGKIAGLYDLEETLGSGHFAVVKLARHVFTGAKVAVKVVDKTKLDDVSKAHLFQEVRCMKLVQHPNVVRLYEVIDTQTKLYLVLELGDGGDLYDYIMKHDSGLSEELARKYFRQILRAITYCHQLHVVHRDLKPENVVFFEKLGLVKLTDFGFSNKFLPGQKLETFCGSLAYSAPEILLGDSYDAPAVDIWSLGVILYMLVCGQAPFEKANDSETLTMIMDCKYTVPSHVSTDCRDLIASMLVRDPKKRATVEEIASSAWLKPIDEPDSTTSTSEHFLPLVSREQLGEEDHAFIIQKMINGNIASKEEILQALDKNKYNHITATYFLLAELRLRRRREELAQKQRLLNDASMKVGDASRKLVPEKPSPTEAQKGGVPISINVTPAAQFANDGGKPDKRSRKCSIVREEDEEESATECTAVGNELKVTSSSRREAFSDSPFSRSIHERSSSEPGNQTKGEQIANSGVPSHSRTKIVVSVDATLAHKLKQMEKSAKIDEDTLSGLKELEIGKLKPLPSSSKNPVLTHRRTKLNKIRTPSCSSSEASDDDTKTRNKKKINKFVGDTPVRFRMHRRDSHDDSSDSQDQLYPPPGSASNAGNFISKSGSVSGKKEGQSQYKEPNNKSDENRKSHSQKNRKQHNKDHVDKHSHAERQLADSSTATTGNSTNRRRRIRESQSLDRITEAQEYEMRHRCYAESEAASSSSSSTQHIDQRYSLLSLNTSTFSVAETKEEYDEEADATNATDQIMNTLNAAKATLQQEQYFNNNTNLSRNFNHNHNQSIIHISSQKANGKKSNETPKDIYNLNSIEHIDLILEDKSLTKAIHVTGSKCFVTMKKIRRLGKYFPVMNFS

Target ORF information:

RefSeq Version	NM_166042.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8485), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166042.2

ORF Insert Sequence:

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8485 ( NM_137098.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610942.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8485), mRNA.

Price & Availability↑

CloneID	OFa09949
Clone ID Related Accession (Same CDS sequence)	NM_166042.2 , NM_137098.3 , NM_166041.2
Accession Version	NM_137098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137098.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610942.2
CDS	323..2905
Translation	MTLETQPVGGVSDGKIAGLYDLEETLGSGHFAVVKLARHVFTGAKVAVKVVDKTKLDDVSKAHLFQEVRCMKLVQHPNVVRLYEVIDTQTKLYLVLELGDGGDLYDYIMKHDSGLSEELARKYFRQILRAITYCHQLHVVHRDLKPENVVFFEKLGLVKLTDFGFSNKFLPGQKLETFCGSLAYSAPEILLGDSYDAPAVDIWSLGVILYMLVCGQAPFEKANDSETLTMIMDCKYTVPSHVSTDCRDLIASMLVRDPKKRATVEEIASSAWLKPIDEPDSTTSTSEHFLPLVSREQLGEEDHAFIIQKMINGNIASKEEILQALDKNKYNHITATYFLLAELRLRRRREELAQKQRLLNDASMKVGDASRKLVPEKPSPTEAQKGGVPISINVTPAAQFANDGGKPDKRSRKCSIVREEDEEESATECTAVGNELKVTSSSRREAFSDSPFSRSIHERSSSEPGNQTKGEQIANSGVPSHSRTKIVVSVDATLAHKLKQMEKSAKIDEDTLSGLKELEIGKLKPLPSSSKNPVLTHRRTKLNKIRTPSCSSSEASDDDTKTRNKKKINKFVGDTPVRFRMHRRDSHDDSSDSQDQLYPPPGSASNAGNFISKSGSVSGKKEGQSQYKEPNNKSDENRKSHSQKNRKQHNKDHVDKHSHAERQLADSSTATTGNSTNRRRRIRESQSLDRITEAQEYEMRHRCYAESEAASSSSSSTQHIDQRYSLLSLNTSTFSVAETKEEYDEEADATNATDQIMNTLNAAKATLQQEQYFNNNTNLSRNFNHNHNQSIIHISSQKANGKKSNETPKDIYNLNSIEHIDLILEDKSLTKAIHVTGSKCFVTMKKIRRLGKYFPVMNFS

Target ORF information:

RefSeq Version	NM_137098.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8485), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137098.3

ORF Insert Sequence:

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8485 ( NM_166041.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725370.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8485), mRNA.

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CloneID	OFa09949
Clone ID Related Accession (Same CDS sequence)	NM_166042.2 , NM_137098.3 , NM_166041.2
Accession Version	NM_166041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166041.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725370.1
CDS	115..2697
Translation	MTLETQPVGGVSDGKIAGLYDLEETLGSGHFAVVKLARHVFTGAKVAVKVVDKTKLDDVSKAHLFQEVRCMKLVQHPNVVRLYEVIDTQTKLYLVLELGDGGDLYDYIMKHDSGLSEELARKYFRQILRAITYCHQLHVVHRDLKPENVVFFEKLGLVKLTDFGFSNKFLPGQKLETFCGSLAYSAPEILLGDSYDAPAVDIWSLGVILYMLVCGQAPFEKANDSETLTMIMDCKYTVPSHVSTDCRDLIASMLVRDPKKRATVEEIASSAWLKPIDEPDSTTSTSEHFLPLVSREQLGEEDHAFIIQKMINGNIASKEEILQALDKNKYNHITATYFLLAELRLRRRREELAQKQRLLNDASMKVGDASRKLVPEKPSPTEAQKGGVPISINVTPAAQFANDGGKPDKRSRKCSIVREEDEEESATECTAVGNELKVTSSSRREAFSDSPFSRSIHERSSSEPGNQTKGEQIANSGVPSHSRTKIVVSVDATLAHKLKQMEKSAKIDEDTLSGLKELEIGKLKPLPSSSKNPVLTHRRTKLNKIRTPSCSSSEASDDDTKTRNKKKINKFVGDTPVRFRMHRRDSHDDSSDSQDQLYPPPGSASNAGNFISKSGSVSGKKEGQSQYKEPNNKSDENRKSHSQKNRKQHNKDHVDKHSHAERQLADSSTATTGNSTNRRRRIRESQSLDRITEAQEYEMRHRCYAESEAASSSSSSTQHIDQRYSLLSLNTSTFSVAETKEEYDEEADATNATDQIMNTLNAAKATLQQEQYFNNNTNLSRNFNHNHNQSIIHISSQKANGKKSNETPKDIYNLNSIEHIDLILEDKSLTKAIHVTGSKCFVTMKKIRRLGKYFPVMNFS

Target ORF information:

RefSeq Version	NM_166041.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8485), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166041.2

ORF Insert Sequence:

ATGACGCTGG AGACGCAGCC CGTTGGCGGG GTGAGCGACG GCAAGATCGC TGGTCTTTAT 
GATCTGGAGG AGACACTGGG ATCCGGACAC TTTGCCGTCG TGAAGTTGGC CCGGCATGTG 
TTCACCGGTG CCAAGGTGGC GGTCAAGGTA GTGGACAAAA CCAAGTTGGA CGATGTGTCC 
AAGGCGCATC TGTTCCAGGA AGTGCGATGC ATGAAACTGG TGCAGCACCC AAATGTTGTG 
CGACTGTACG AGGTTATTGA TACCCAGACC AAGCTGTATT TGGTTTTGGA GCTTGGCGAT 
GGTGGCGATC TATACGATTA CATAATGAAG CACGACTCTG GGCTCAGCGA GGAACTGGCC 
CGAAAGTATT TCCGCCAAAT CCTACGTGCT ATCACGTACT GTCATCAGCT TCACGTGGTT 
CACAGGGATC TAAAGCCGGA GAACGTGGTG TTTTTCGAAA AGCTGGGCCT TGTCAAGCTC 
ACCGACTTCG GGTTTAGCAA CAAGTTCTTG CCGGGCCAAA AGCTAGAAAC CTTCTGCGGC 
AGCTTGGCCT ACTCGGCCCC AGAGATCCTG CTGGGCGACT CCTATGATGC GCCTGCAGTT 
GACATTTGGT CCCTGGGAGT AATTCTGTAT ATGCTGGTCT GCGGTCAGGC GCCTTTCGAG 
AAGGCCAACG ACTCGGAAAC TCTGACCATG ATCATGGATT GTAAGTACAC GGTACCCTCG 
CACGTGTCCA CTGATTGTCG GGACCTGATT GCCTCAATGC TGGTGCGGGA TCCAAAGAAA 
CGGGCCACTG TGGAAGAGAT TGCTTCATCG GCTTGGCTAA AGCCCATCGA TGAGCCCGAT 
TCGACGACCT CCACCTCAGA GCACTTTCTG CCTTTGGTCA GTCGCGAACA GCTGGGCGAA 
GAGGATCACG CCTTTATCAT ACAAAAAATG ATAAACGGAA ACATTGCGTC CAAGGAAGAG 
ATATTGCAAG CTCTTGACAA AAATAAGTAC AATCATATAA CAGCCACATA TTTTTTGTTG 
GCGGAATTAC GTCTGCGACG GCGACGAGAA GAGCTGGCTC AAAAACAGAG GCTGCTCAAC 
GATGCCAGCA TGAAAGTGGG AGATGCGAGT CGCAAGCTAG TGCCTGAGAA ACCAAGTCCA 
ACCGAAGCCC AAAAAGGAGG AGTACCCATC AGCATTAACG TGACTCCAGC AGCTCAGTTT 
GCCAATGACG GTGGCAAGCC GGACAAGCGC AGTCGCAAGT GCAGCATTGT GCGTGAGGAG 
GACGAAGAGG AGTCTGCCAC AGAATGCACG GCGGTAGGCA ATGAGTTGAA GGTAACGTCG 
TCGTCGCGGC GCGAGGCCTT CTCAGATTCC CCCTTTAGTA GGTCAATACA TGAGCGTTCC 
AGCTCTGAGC CCGGAAATCA AACGAAAGGC GAACAGATAG CTAATAGCGG AGTGCCAAGC 
CATTCACGTA CAAAGATTGT CGTCTCGGTG GATGCAACGC TGGCCCATAA GCTCAAGCAG 
ATGGAGAAGA GTGCCAAGAT CGATGAGGAC ACGCTGAGTG GTCTGAAGGA GCTGGAGATC 
GGCAAGCTCA AGCCGCTTCC TAGCAGCAGC AAGAATCCGG TGCTTACCCA CCGTCGCACA 
AAGCTGAACA AGATTCGGAC GCCTTCATGC AGCAGCTCGG AGGCCTCAGA CGACGACACC 
AAGACACGCA ACAAGAAAAA GATTAACAAA TTTGTCGGTG ACACGCCAGT GCGGTTTCGC 
ATGCATCGTC GCGACTCGCA TGACGACTCC AGCGACTCGC AAGATCAGCT ATATCCGCCG 
CCCGGCTCGG CCAGCAATGC GGGTAATTTT ATCTCCAAAA GCGGCTCAGT AAGCGGCAAA 
AAAGAGGGAC AATCGCAGTA TAAAGAGCCA AATAACAAAT CCGATGAAAA TCGCAAATCT 
CACAGCCAAA AAAATCGGAA ACAGCACAAC AAGGATCATG TTGATAAGCA TTCCCACGCT 
GAGAGGCAGC TGGCGGATAG CAGCACAGCT ACAACAGGAA ATTCCACGAA TAGAAGGCGG 
CGCATTCGCG AGAGCCAGTC GCTGGACCGC ATCACAGAGG CTCAGGAATA CGAGATGCGG 
CATCGATGCT ACGCGGAGAG CGAGGCAGCA TCCTCGTCGT CCTCGTCCAC CCAACATATC 
GATCAGAGAT ATTCTTTACT CAGCCTAAAC ACTAGCACAT TCTCCGTTGC CGAAACCAAG 
GAGGAGTACG ACGAGGAGGC AGACGCCACA AATGCCACGG ACCAAATCAT GAATACTTTG 
AATGCAGCCA AAGCAACGCT TCAACAAGAG CAATATTTTA ATAACAATAC CAATCTTAGT 
AGGAACTTCA ATCACAATCA CAACCAGAGC ATCATCCACA TCAGTAGCCA GAAAGCCAAC 
GGCAAGAAGT CCAACGAAAC ACCAAAAGAT ATTTATAATC TGAACTCAAT CGAGCATATC 
GACTTGATTT TGGAGGATAA AAGCCTTACC AAAGCAATAC ACGTTACCGG CTCTAAATGC 
TTTGTCACCA TGAAGAAAAT CCGACGGCTG GGAAAATATT TTCCCGTGAT GAACTTCTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8485 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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