Opa1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Opa1
Entrez Gene ID	36578
Full Name	Optic atrophy 1
Synonyms	CG8479,Dmel\CG8479,OPA1,OPA1-like,Opa1-like,dOPA1,dOpa1,l(2)s3475,opa1,opa1-like
General protein information	Preferred Names Optic atrophy 1 Names CG8479 gene product from transcript CG8479-RD CG8479-PA CG8479-PB CG8479-PC CG8479-PD CG8479-PE Opa1-PA Opa1-PB Opa1-PC Opa1-PD Opa1-PE Opa1-like dOpa1 drosophila optic atrophy 1-like lethal (2) s3475 opa1 optic atrophy 1 optic atrophy 1 ortholog optic atrophy 1-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50E4-50E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_137097.3	NP_610941.1	optic atrophy 1, isoform B
NM_166040.2	NP_725369.1	optic atrophy 1, isoform A
NM_001299483.1	NP_001286412.1	optic atrophy 1, isoform D
NM_001299484.1	NP_001286413.1	optic atrophy 1, isoform E
NM_001299482.1	NP_001286411.1	optic atrophy 1, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Opa1	NP_598269.3
Danio rerio (zebrafish)	LOC100307079	XP_005166050.1
Pan troglodytes (chimpanzee)	OPA1	XP_003310225.1
Bos taurus (cattle)	OPA1	NP_001179890.1
Mus musculus (house mouse)	Opa1	NP_001186106.1
Danio rerio (zebrafish)	opa1	NP_001007299.1
Homo sapiens (human)	OPA1	NP_570849.2
	OPA1	XP_001087037.2
Drosophila melanogaster (fruit fly)	opa1-like	NP_725369.1
Canis lupus familiaris (dog)	LOC477129	XP_005634679.1
Gallus gallus (chicken)	OPA1	NP_001034398.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011286	XP_309360.3
Xenopus tropicalis (tropical clawed frog)	opa1	NP_001120510.1
Caenorhabditis elegans (roundworm)	eat-3	NP_495986.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lack of ChChd3 leads to inactivation of Hippo activity under normal development, which is also dependent on the transcriptional coactivator Yorkie (Yki). Furthermore, loss of ChChd3 induces oxidative stress and activates the JNK pathway. In addition, depletion of other mitochondrial fusion components, Opa1 or Marf, inactivates the Hippo pathway as well.
Title: Cross-Talk Between Mitochondrial Fusion and the Hippo Pathway in Controlling Cell Proliferation During Drosophila Development.

Mitochondrial fusion is regulated spatiotemporally by Ewg through Opa1-like during indirect flight muscles differentiation and growth.
Title: Drosophila Erect wing (Ewg) controls mitochondrial fusion during muscle growth and maintenance by regulation of the Opa1-like gene.

these results suggest that rho-7 and Opa1-like function in a common molecular pathway affecting mitochondrial function and apoptosis in Drosophila melanogaster.
Title: Rhomboid-7 over-expression results in Opa1-like processing and malfunctioning mitochondria.

MARF and Opa1 control mitochondrial and cardiac function in Drosophila.
Title: MARF and Opa1 control mitochondrial and cardiac function in Drosophila.

results suggest that heterozygous mutation of dOpa1 shows organ-specific pathogenesis and is associated with multiple organ abnormalities in an age-dependent and organ-specific manner
Title: Heterozygous mutation of Drosophila Opa1 causes the development of multiple organ abnormalities in an age-dependent and organ-specific manner.

The following Opa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Opa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21804	NM_001299484.1 Latest version!	Drosophila melanogaster optic atrophy 1, transcript variant E (Opa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09947	NM_137097.3 Latest version!	Drosophila melanogaster optic atrophy 1, transcript variant B (Opa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09948	NM_166040.2 Latest version!	Drosophila melanogaster optic atrophy 1, transcript variant A (Opa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21803	NM_001299483.1 Latest version!	Drosophila melanogaster optic atrophy 1, transcript variant D (Opa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21805	NM_001299482.1 Latest version!	Drosophila melanogaster optic atrophy 1, transcript variant C (Opa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Opa1 ( NM_001299484.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286413.1 Drosophila melanogaster optic atrophy 1, transcript variant E (Opa1), mRNA.

Price & Availability↑

CloneID	OFa21804
Clone ID Related Accession (Same CDS sequence)	NM_001299484.1
Accession Version	NM_001299484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optic atrophy 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGGTTC TCAAAGTAAT 
ATTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286413.1
CDS	136..2004
Translation	MLRIYQNTYRRTARKAVVYSTKVACCNHSTLCGITSHPRRAQDSGSSSSNGRHRGHEEFLLAGNPARGWQMPPPSRGYGMLVVRILRGALKLRYIVLGGAIGGGVSLSKKYEEWKDGLPNFKWLEDAMPQGERWSQFSRNLIEVGSLVKNAIEVAKDDLKAKTTVAALGITSDESRKKYEKLQSQVETLQTEIMNVQIKYQKELEKMEKENRELRQQYLILKTNKKTTAKKIKKSLIDMYSEVLDELSGYDTGYTMADHLPRVVVVGDQSSGKTSVLESIAKARIFPRGSGEMMTRAPVKVTLAEGPYHVAQFRDSDREYDLTKESDLQDLRRDVEFRMKASVRGGKTVSNEVIAMTVKGPGLQRMVLVDLPGIISTMTVDMASDTKDSIHQMTKHYMSNPNAIILCIQDGSVDAERSNVTDLVMQCDPLGRRTIFVLTKVDLAEELADPDRIRKILSGKLFPMKALGYYAVVTGRGRKDDSIDAIRQYEEDFFKNSKLFHRRGVIMPHQVTSRNLSLAVSDRFWKMVRETIEQQADAFKATRFNLETEWKNNFPRLRESGRDELFDKAKGEILDEVVTLSQISAKKWDDALSTKLWEKLSNYVFESIYLPAAQSGSQSNIL

Target ORF information:

RefSeq Version	NM_001299484.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optic atrophy 1, transcript variant E (Opa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299484.1

ORF Insert Sequence:

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGGTTC TCAAAGTAAT 
ATTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Opa1 ( NM_137097.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610941.1 Drosophila melanogaster optic atrophy 1, transcript variant B (Opa1), mRNA.

Price & Availability↑

CloneID	OFa09947
Clone ID Related Accession (Same CDS sequence)	NM_137097.3
Accession Version	NM_137097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optic atrophy 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137097.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGGTTC TCAAAATTCC 
TTCAACACGA TGGTAGACAT CAAGTTGCGC CAGTGGGCCG AGCAGGCACT GCCCGCTAAG 
TCGGTGGAAG CTGGCTGGGA AGCGTTGCAG CAGGAATTCA TATCGCTGAT GGAGCGTTCG 
AAGAAGGCGC AGGATCACGA CGGCATCTTC GATCAGTTGA AGTCTGCGGT GGTGGATGAG 
GCTATACGTC GGCACAGCTG GGAGGACAAG GCCATCGACA TGCTTCGCGT TATCCAGCTG 
AATACGCTGG AGGATCGGTT CGTGCACGAC AAGCAGGAGT GGGATTCGGC GGTTAAGTTC 
CTGGAGAGTT CGGTGAATGC CAAGCTCGTG CAGACGGAGG AGACTTTAGC ACAAATGTTT 
GGTCCCGGCC AAATGCGACG AATTACCCAC TGGCAGTACC TGACGCAGGA TCAGCAGAAG 
AGGCGAAGCG TTAAGAACGA ACTCGACAAA ATACTCAAAA ACGATACGAA ACATTTGCCC 
ACCCTGACCC ATGATGAACT GACTACGGTT CGCAAGAACC TTCAGCGTGA TAACGTGGAT 
GTGGACACGG ACTACATAAG GCAAACGTGG TTCCCGGTCT ACAGAAAACA CTTTCTGCAG 
CAGGCGTTAC AGAGGGCAAA GGATTGCCGC AAAGCATATT ACCTCTACAC GCAGCAGGGA 
GCCGAGTGTG AGATATCCTG CAGCGACGTC GTGCTCTTCT GGCGCATCCA GCAGGTGATC 
AAGATAACAG GCAACGCACT GAGACAGCAA GTAATCAATC GGGAGGCGCG GCGGCTGGAC 
AAGGAGATCA AAGCGGTGCT GGACGAGTTC AGCGATGACG AGGAGAAGAA GGGTTACCTG 
CTCACCGGCA AGCGCGTGCT ACTAGCCGAG GAGCTAATCA AAGTGCGGCA GATCCAAGAG 
AAGCTGGAGG AGTTCATCAA TTCACTTAAT CAGGAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610941.1
CDS	136..2937
Translation	MLRIYQNTYRRTARKAVVYSTKVACCNHSTLCGITSHPRRAQDSGSSSSNGRHRGHEEFLLAGNPARGWQMPPPSRGYGMLVVRILRGALKLRYIVLGGAIGGGVSLSKKYEEWKDGLPNFKWLEDAMPQGERWSQFSRNLIEVGSLVKNAIEVAKDDLKAKTTVAALGITSDESRKKYEKLQSQVETLQTEIMNVQIKYQKELEKMEKENRELRQQYLILKTNKKTTAKKIKKSLIDMYSEVLDELSGYDTGYTMADHLPRVVVVGDQSSGKTSVLESIAKARIFPRGSGEMMTRAPVKVTLAEGPYHVAQFRDSDREYDLTKESDLQDLRRDVEFRMKASVRGGKTVSNEVIAMTVKGPGLQRMVLVDLPGIISTMTVDMASDTKDSIHQMTKHYMSNPNAIILCIQDGSVDAERSNVTDLVMQCDPLGRRTIFVLTKVDLAEELADPDRIRKILSGKLFPMKALGYYAVVTGRGRKDDSIDAIRQYEEDFFKNSKLFHRRGVIMPHQVTSRNLSLAVSDRFWKMVRETIEQQADAFKATRFNLETEWKNNFPRLRESGRDELFDKAKGEILDEVVTLSQISAKKWDDALSTKLWEKLSNYVFESIYLPAAQSGSQNSFNTMVDIKLRQWAEQALPAKSVEAGWEALQQEFISLMERSKKAQDHDGIFDQLKSAVVDEAIRRHSWEDKAIDMLRVIQLNTLEDRFVHDKQEWDSAVKFLESSVNAKLVQTEETLAQMFGPGQMRRITHWQYLTQDQQKRRSVKNELDKILKNDTKHLPTLTHDELTTVRKNLQRDNVDVDTDYIRQTWFPVYRKHFLQQALQRAKDCRKAYYLYTQQGAECEISCSDVVLFWRIQQVIKITGNALRQQVINREARRLDKEIKAVLDEFSDDEEKKGYLLTGKRVLLAEELIKVRQIQEKLEEFINSLNQEK

Target ORF information:

RefSeq Version	NM_137097.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optic atrophy 1, transcript variant B (Opa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137097.3

ORF Insert Sequence:

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGGTTC TCAAAATTCC 
TTCAACACGA TGGTAGACAT CAAGTTGCGC CAGTGGGCCG AGCAGGCACT GCCCGCTAAG 
TCGGTGGAAG CTGGCTGGGA AGCGTTGCAG CAGGAATTCA TATCGCTGAT GGAGCGTTCG 
AAGAAGGCGC AGGATCACGA CGGCATCTTC GATCAGTTGA AGTCTGCGGT GGTGGATGAG 
GCTATACGTC GGCACAGCTG GGAGGACAAG GCCATCGACA TGCTTCGCGT TATCCAGCTG 
AATACGCTGG AGGATCGGTT CGTGCACGAC AAGCAGGAGT GGGATTCGGC GGTTAAGTTC 
CTGGAGAGTT CGGTGAATGC CAAGCTCGTG CAGACGGAGG AGACTTTAGC ACAAATGTTT 
GGTCCCGGCC AAATGCGACG AATTACCCAC TGGCAGTACC TGACGCAGGA TCAGCAGAAG 
AGGCGAAGCG TTAAGAACGA ACTCGACAAA ATACTCAAAA ACGATACGAA ACATTTGCCC 
ACCCTGACCC ATGATGAACT GACTACGGTT CGCAAGAACC TTCAGCGTGA TAACGTGGAT 
GTGGACACGG ACTACATAAG GCAAACGTGG TTCCCGGTCT ACAGAAAACA CTTTCTGCAG 
CAGGCGTTAC AGAGGGCAAA GGATTGCCGC AAAGCATATT ACCTCTACAC GCAGCAGGGA 
GCCGAGTGTG AGATATCCTG CAGCGACGTC GTGCTCTTCT GGCGCATCCA GCAGGTGATC 
AAGATAACAG GCAACGCACT GAGACAGCAA GTAATCAATC GGGAGGCGCG GCGGCTGGAC 
AAGGAGATCA AAGCGGTGCT GGACGAGTTC AGCGATGACG AGGAGAAGAA GGGTTACCTG 
CTCACCGGCA AGCGCGTGCT ACTAGCCGAG GAGCTAATCA AAGTGCGGCA GATCCAAGAG 
AAGCTGGAGG AGTTCATCAA TTCACTTAAT CAGGAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Opa1 ( NM_166040.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725369.1 Drosophila melanogaster optic atrophy 1, transcript variant A (Opa1), mRNA.

Price & Availability↑

CloneID	OFa09948
Clone ID Related Accession (Same CDS sequence)	NM_166040.2
Accession Version	NM_166040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optic atrophy 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166040.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGATCCAAA GCTCAAGCAG 
CTGGGCGAGG ATAAGTTGTC GGAATGGCGC AACTGGTTTG ACAGTCGTCT GGACGATGCC 
ATCGAGGCGG CCGATTATCA AGGAGTTCAG ATTGTCGAAA CCAAAGATGA CTTAAAGGCC 
AAAACAACGG TGGCCGCTTT AGGCATAACA TCCGATGAGA GTCGTAAGAA GTATGAGAAA 
CTCCAGAGCC AGGTGGAGAC GCTGCAGACG GAGATCATGA ACGTCCAGAT TAAGTACCAA 
AAGGAGCTGG AGAAGATGGA GAAGGAGAAC CGTGAACTAC GCCAGCAATA CCTCATCCTG 
AAAACAAACA AAAAGACCAC GGCAAAAAAA ATCAAAAAAT CCCTGATCGA CATGTACTCT 
GAGGTCCTTG ATGAGCTATC CGGCTACGAT ACGGGCTACA CCATGGCCGA TCACCTACCC 
CGTGTTGTGG TAGTGGGAGA TCAGAGCAGC GGCAAGACCT CTGTCCTGGA ATCCATTGCT 
AAGGCTCGCA TCTTTCCCCG TGGCAGTGGA GAGATGATGA CGCGAGCCCC AGTCAAAGTT 
ACTCTGGCTG AAGGACCATA CCATGTGGCT CAGTTCCGTG ACTCTGACCG GGAATACGAT 
CTCACCAAGG AGTCTGATCT GCAGGACCTC CGTCGGGATG TGGAGTTCCG AATGAAGGCG 
TCGGTGAGAG GTGGTAAAAC CGTCAGCAAT GAAGTCATTG CCATGACGGT CAAGGGTCCC 
GGTCTGCAAC GCATGGTTCT AGTCGATTTG CCAGGAATAA TTTCGACCAT GACTGTCGAC 
ATGGCCTCAG ACACAAAAGA TTCCATTCAC CAGATGACCA AGCATTATAT GAGCAATCCG 
AACGCCATCA TTCTCTGCAT TCAGGATGGA TCTGTGGACG CTGAGCGCAG TAATGTGACA 
GACTTGGTCA TGCAGTGCGA TCCCTTGGGT CGACGCACTA TTTTTGTGCT CACAAAGGTG 
GATCTGGCCG AGGAGCTCGC CGATCCTGAT AGAATAAGGA AAATCCTTTC GGGCAAACTC 
TTCCCCATGA AGGCTTTGGG CTACTATGCC GTCGTTACCG GTCGTGGACG AAAGGATGAC 
AGCATAGATG CTATTCGGCA GTATGAGGAA GACTTCTTTA AGAACTCCAA GCTTTTCCAT 
CGTCGAGGGG TTATCATGCC CCATCAGGTG ACCAGCCGCA ATCTGAGCTT GGCGGTGTCA 
GATCGTTTCT GGAAGATGGT GAGGGAAACC ATTGAGCAGC AGGCGGATGC ATTTAAGGCG 
ACTAGATTTA ATCTGGAAAC CGAATGGAAA AATAACTTCC CCAGATTGCG CGAGTCTGGT 
CGAGATGAGC TGTTCGACAA GGCAAAGGGC GAGATACTGG ACGAGGTGGT AACGCTCTCG 
CAAATCTCCG CTAAAAAGTG GGACGACGCT CTGAGCACCA AGCTATGGGA AAAGCTCTCC 
AATTATGTGT TTGAAAGCAT CTATCTGCCC GCTGCACAGT CAGGTTCTCA AAATTCCTTC 
AACACGATGG TAGACATCAA GTTGCGCCAG TGGGCCGAGC AGGCACTGCC CGCTAAGTCG 
GTGGAAGCTG GCTGGGAAGC GTTGCAGCAG GAATTCATAT CGCTGATGGA GCGTTCGAAG 
AAGGCGCAGG ATCACGACGG CATCTTCGAT CAGTTGAAGT CTGCGGTGGT GGATGAGGCT 
ATACGTCGGC ACAGCTGGGA GGACAAGGCC ATCGACATGC TTCGCGTTAT CCAGCTGAAT 
ACGCTGGAGG ATCGGTTCGT GCACGACAAG CAGGAGTGGG ATTCGGCGGT TAAGTTCCTG 
GAGAGTTCGG TGAATGCCAA GCTCGTGCAG ACGGAGGAGA CTTTAGCACA AATGTTTGGT 
CCCGGCCAAA TGCGACGAAT TACCCACTGG CAGTACCTGA CGCAGGATCA GCAGAAGAGG 
CGAAGCGTTA AGAACGAACT CGACAAAATA CTCAAAAACG ATACGAAACA TTTGCCCACC 
CTGACCCATG ATGAACTGAC TACGGTTCGC AAGAACCTTC AGCGTGATAA CGTGGATGTG 
GACACGGACT ACATAAGGCA AACGTGGTTC CCGGTCTACA GAAAACACTT TCTGCAGCAG 
GCGTTACAGA GGGCAAAGGA TTGCCGCAAA GCATATTACC TCTACACGCA GCAGGGAGCC 
GAGTGTGAGA TATCCTGCAG CGACGTCGTG CTCTTCTGGC GCATCCAGCA GGTGATCAAG 
ATAACAGGCA ACGCACTGAG ACAGCAAGTA ATCAATCGGG AGGCGCGGCG GCTGGACAAG 
GAGATCAAAG CGGTGCTGGA CGAGTTCAGC GATGACGAGG AGAAGAAGGG TTACCTGCTC 
ACCGGCAAGC GCGTGCTACT AGCCGAGGAG CTAATCAAAG TGCGGCAGAT CCAAGAGAAG 
CTGGAGGAGT TCATCAATTC ACTTAATCAG GAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725369.1
CDS	136..3054
Translation	MLRIYQNTYRRTARKAVVYSTKVACCNHSTLCGITSHPRRAQDSGSSSSNGRHRGHEEFLLAGNPARGWQMPPPSRGYGMLVVRILRGALKLRYIVLGGAIGGGVSLSKKYEEWKDGLPNFKWLEDAMPQGERWSQFSRNLIEVGSLVKNAIEVDPKLKQLGEDKLSEWRNWFDSRLDDAIEAADYQGVQIVETKDDLKAKTTVAALGITSDESRKKYEKLQSQVETLQTEIMNVQIKYQKELEKMEKENRELRQQYLILKTNKKTTAKKIKKSLIDMYSEVLDELSGYDTGYTMADHLPRVVVVGDQSSGKTSVLESIAKARIFPRGSGEMMTRAPVKVTLAEGPYHVAQFRDSDREYDLTKESDLQDLRRDVEFRMKASVRGGKTVSNEVIAMTVKGPGLQRMVLVDLPGIISTMTVDMASDTKDSIHQMTKHYMSNPNAIILCIQDGSVDAERSNVTDLVMQCDPLGRRTIFVLTKVDLAEELADPDRIRKILSGKLFPMKALGYYAVVTGRGRKDDSIDAIRQYEEDFFKNSKLFHRRGVIMPHQVTSRNLSLAVSDRFWKMVRETIEQQADAFKATRFNLETEWKNNFPRLRESGRDELFDKAKGEILDEVVTLSQISAKKWDDALSTKLWEKLSNYVFESIYLPAAQSGSQNSFNTMVDIKLRQWAEQALPAKSVEAGWEALQQEFISLMERSKKAQDHDGIFDQLKSAVVDEAIRRHSWEDKAIDMLRVIQLNTLEDRFVHDKQEWDSAVKFLESSVNAKLVQTEETLAQMFGPGQMRRITHWQYLTQDQQKRRSVKNELDKILKNDTKHLPTLTHDELTTVRKNLQRDNVDVDTDYIRQTWFPVYRKHFLQQALQRAKDCRKAYYLYTQQGAECEISCSDVVLFWRIQQVIKITGNALRQQVINREARRLDKEIKAVLDEFSDDEEKKGYLLTGKRVLLAEELIKVRQIQEKLEEFINSLNQEK

Target ORF information:

RefSeq Version	NM_166040.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optic atrophy 1, transcript variant A (Opa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166040.2

ORF Insert Sequence:

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGATCCAAA GCTCAAGCAG 
CTGGGCGAGG ATAAGTTGTC GGAATGGCGC AACTGGTTTG ACAGTCGTCT GGACGATGCC 
ATCGAGGCGG CCGATTATCA AGGAGTTCAG ATTGTCGAAA CCAAAGATGA CTTAAAGGCC 
AAAACAACGG TGGCCGCTTT AGGCATAACA TCCGATGAGA GTCGTAAGAA GTATGAGAAA 
CTCCAGAGCC AGGTGGAGAC GCTGCAGACG GAGATCATGA ACGTCCAGAT TAAGTACCAA 
AAGGAGCTGG AGAAGATGGA GAAGGAGAAC CGTGAACTAC GCCAGCAATA CCTCATCCTG 
AAAACAAACA AAAAGACCAC GGCAAAAAAA ATCAAAAAAT CCCTGATCGA CATGTACTCT 
GAGGTCCTTG ATGAGCTATC CGGCTACGAT ACGGGCTACA CCATGGCCGA TCACCTACCC 
CGTGTTGTGG TAGTGGGAGA TCAGAGCAGC GGCAAGACCT CTGTCCTGGA ATCCATTGCT 
AAGGCTCGCA TCTTTCCCCG TGGCAGTGGA GAGATGATGA CGCGAGCCCC AGTCAAAGTT 
ACTCTGGCTG AAGGACCATA CCATGTGGCT CAGTTCCGTG ACTCTGACCG GGAATACGAT 
CTCACCAAGG AGTCTGATCT GCAGGACCTC CGTCGGGATG TGGAGTTCCG AATGAAGGCG 
TCGGTGAGAG GTGGTAAAAC CGTCAGCAAT GAAGTCATTG CCATGACGGT CAAGGGTCCC 
GGTCTGCAAC GCATGGTTCT AGTCGATTTG CCAGGAATAA TTTCGACCAT GACTGTCGAC 
ATGGCCTCAG ACACAAAAGA TTCCATTCAC CAGATGACCA AGCATTATAT GAGCAATCCG 
AACGCCATCA TTCTCTGCAT TCAGGATGGA TCTGTGGACG CTGAGCGCAG TAATGTGACA 
GACTTGGTCA TGCAGTGCGA TCCCTTGGGT CGACGCACTA TTTTTGTGCT CACAAAGGTG 
GATCTGGCCG AGGAGCTCGC CGATCCTGAT AGAATAAGGA AAATCCTTTC GGGCAAACTC 
TTCCCCATGA AGGCTTTGGG CTACTATGCC GTCGTTACCG GTCGTGGACG AAAGGATGAC 
AGCATAGATG CTATTCGGCA GTATGAGGAA GACTTCTTTA AGAACTCCAA GCTTTTCCAT 
CGTCGAGGGG TTATCATGCC CCATCAGGTG ACCAGCCGCA ATCTGAGCTT GGCGGTGTCA 
GATCGTTTCT GGAAGATGGT GAGGGAAACC ATTGAGCAGC AGGCGGATGC ATTTAAGGCG 
ACTAGATTTA ATCTGGAAAC CGAATGGAAA AATAACTTCC CCAGATTGCG CGAGTCTGGT 
CGAGATGAGC TGTTCGACAA GGCAAAGGGC GAGATACTGG ACGAGGTGGT AACGCTCTCG 
CAAATCTCCG CTAAAAAGTG GGACGACGCT CTGAGCACCA AGCTATGGGA AAAGCTCTCC 
AATTATGTGT TTGAAAGCAT CTATCTGCCC GCTGCACAGT CAGGTTCTCA AAATTCCTTC 
AACACGATGG TAGACATCAA GTTGCGCCAG TGGGCCGAGC AGGCACTGCC CGCTAAGTCG 
GTGGAAGCTG GCTGGGAAGC GTTGCAGCAG GAATTCATAT CGCTGATGGA GCGTTCGAAG 
AAGGCGCAGG ATCACGACGG CATCTTCGAT CAGTTGAAGT CTGCGGTGGT GGATGAGGCT 
ATACGTCGGC ACAGCTGGGA GGACAAGGCC ATCGACATGC TTCGCGTTAT CCAGCTGAAT 
ACGCTGGAGG ATCGGTTCGT GCACGACAAG CAGGAGTGGG ATTCGGCGGT TAAGTTCCTG 
GAGAGTTCGG TGAATGCCAA GCTCGTGCAG ACGGAGGAGA CTTTAGCACA AATGTTTGGT 
CCCGGCCAAA TGCGACGAAT TACCCACTGG CAGTACCTGA CGCAGGATCA GCAGAAGAGG 
CGAAGCGTTA AGAACGAACT CGACAAAATA CTCAAAAACG ATACGAAACA TTTGCCCACC 
CTGACCCATG ATGAACTGAC TACGGTTCGC AAGAACCTTC AGCGTGATAA CGTGGATGTG 
GACACGGACT ACATAAGGCA AACGTGGTTC CCGGTCTACA GAAAACACTT TCTGCAGCAG 
GCGTTACAGA GGGCAAAGGA TTGCCGCAAA GCATATTACC TCTACACGCA GCAGGGAGCC 
GAGTGTGAGA TATCCTGCAG CGACGTCGTG CTCTTCTGGC GCATCCAGCA GGTGATCAAG 
ATAACAGGCA ACGCACTGAG ACAGCAAGTA ATCAATCGGG AGGCGCGGCG GCTGGACAAG 
GAGATCAAAG CGGTGCTGGA CGAGTTCAGC GATGACGAGG AGAAGAAGGG TTACCTGCTC 
ACCGGCAAGC GCGTGCTACT AGCCGAGGAG CTAATCAAAG TGCGGCAGAT CCAAGAGAAG 
CTGGAGGAGT TCATCAATTC ACTTAATCAG GAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Opa1 ( NM_001299483.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286412.1 Drosophila melanogaster optic atrophy 1, transcript variant D (Opa1), mRNA.

Price & Availability↑

CloneID	OFa21803
Clone ID Related Accession (Same CDS sequence)	NM_001299483.1
Accession Version	NM_001299483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optic atrophy 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGATTC CTTCAACACG 
ATGGTAGACA TCAAGTTGCG CCAGTGGGCC GAGCAGGCAC TGCCCGCTAA GTCGGTGGAA 
GCTGGCTGGG AAGCGTTGCA GCAGGAATTC ATATCGCTGA TGGAGCGTTC GAAGAAGGCG 
CAGGATCACG ACGGCATCTT CGATCAGTTG AAGTCTGCGG TGGTGGATGA GGCTATACGT 
CGGCACAGCT GGGAGGACAA GGCCATCGAC ATGCTTCGCG TTATCCAGCT GAATACGCTG 
GAGGATCGGT TCGTGCACGA CAAGCAGGAG TGGGATTCGG CGGTTAAGTT CCTGGAGAGT 
TCGGTGAATG CCAAGCTCGT GCAGACGGAG GAGACTTTAG CACAAATGTT TGGTCCCGGC 
CAAATGCGAC GAATTACCCA CTGGCAGTAC CTGACGCAGG ATCAGCAGAA GAGGCGAAGC 
GTTAAGAACG AACTCGACAA AATACTCAAA AACGATACGA AACATTTGCC CACCCTGACC 
CATGATGAAC TGACTACGGT TCGCAAGAAC CTTCAGCGTG ATAACGTGGA TGTGGACACG 
GACTACATAA GGCAAACGTG GTTCCCGGTC TACAGAAAAC ACTTTCTGCA GCAGGCGTTA 
CAGAGGGCAA AGGATTGCCG CAAAGCATAT TACCTCTACA CGCAGCAGGG AGCCGAGTGT 
GAGATATCCT GCAGCGACGT CGTGCTCTTC TGGCGCATCC AGCAGGTGAT CAAGATAACA 
GGCAACGCAC TGAGACAGCA AGTAATCAAT CGGGAGGCGC GGCGGCTGGA CAAGGAGATC 
AAAGCGGTGC TGGACGAGTT CAGCGATGAC GAGGAGAAGA AGGGTTACCT GCTCACCGGC 
AAGCGCGTGC TACTAGCCGA GGAGCTAATC AAAGTGCGGC AGATCCAAGA GAAGCTGGAG 
GAGTTCATCA ATTCACTTAA TCAGGAAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286412.1
CDS	136..2928
Translation	MLRIYQNTYRRTARKAVVYSTKVACCNHSTLCGITSHPRRAQDSGSSSSNGRHRGHEEFLLAGNPARGWQMPPPSRGYGMLVVRILRGALKLRYIVLGGAIGGGVSLSKKYEEWKDGLPNFKWLEDAMPQGERWSQFSRNLIEVGSLVKNAIEVAKDDLKAKTTVAALGITSDESRKKYEKLQSQVETLQTEIMNVQIKYQKELEKMEKENRELRQQYLILKTNKKTTAKKIKKSLIDMYSEVLDELSGYDTGYTMADHLPRVVVVGDQSSGKTSVLESIAKARIFPRGSGEMMTRAPVKVTLAEGPYHVAQFRDSDREYDLTKESDLQDLRRDVEFRMKASVRGGKTVSNEVIAMTVKGPGLQRMVLVDLPGIISTMTVDMASDTKDSIHQMTKHYMSNPNAIILCIQDGSVDAERSNVTDLVMQCDPLGRRTIFVLTKVDLAEELADPDRIRKILSGKLFPMKALGYYAVVTGRGRKDDSIDAIRQYEEDFFKNSKLFHRRGVIMPHQVTSRNLSLAVSDRFWKMVRETIEQQADAFKATRFNLETEWKNNFPRLRESGRDELFDKAKGEILDEVVTLSQISAKKWDDALSTKLWEKLSNYVFESIYLPAAQSDSFNTMVDIKLRQWAEQALPAKSVEAGWEALQQEFISLMERSKKAQDHDGIFDQLKSAVVDEAIRRHSWEDKAIDMLRVIQLNTLEDRFVHDKQEWDSAVKFLESSVNAKLVQTEETLAQMFGPGQMRRITHWQYLTQDQQKRRSVKNELDKILKNDTKHLPTLTHDELTTVRKNLQRDNVDVDTDYIRQTWFPVYRKHFLQQALQRAKDCRKAYYLYTQQGAECEISCSDVVLFWRIQQVIKITGNALRQQVINREARRLDKEIKAVLDEFSDDEEKKGYLLTGKRVLLAEELIKVRQIQEKLEEFINSLNQEK

Target ORF information:

RefSeq Version	NM_001299483.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optic atrophy 1, transcript variant D (Opa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299483.1

ORF Insert Sequence:

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGCCAAAGA TGACTTAAAG 
GCCAAAACAA CGGTGGCCGC TTTAGGCATA ACATCCGATG AGAGTCGTAA GAAGTATGAG 
AAACTCCAGA GCCAGGTGGA GACGCTGCAG ACGGAGATCA TGAACGTCCA GATTAAGTAC 
CAAAAGGAGC TGGAGAAGAT GGAGAAGGAG AACCGTGAAC TACGCCAGCA ATACCTCATC 
CTGAAAACAA ACAAAAAGAC CACGGCAAAA AAAATCAAAA AATCCCTGAT CGACATGTAC 
TCTGAGGTCC TTGATGAGCT ATCCGGCTAC GATACGGGCT ACACCATGGC CGATCACCTA 
CCCCGTGTTG TGGTAGTGGG AGATCAGAGC AGCGGCAAGA CCTCTGTCCT GGAATCCATT 
GCTAAGGCTC GCATCTTTCC CCGTGGCAGT GGAGAGATGA TGACGCGAGC CCCAGTCAAA 
GTTACTCTGG CTGAAGGACC ATACCATGTG GCTCAGTTCC GTGACTCTGA CCGGGAATAC 
GATCTCACCA AGGAGTCTGA TCTGCAGGAC CTCCGTCGGG ATGTGGAGTT CCGAATGAAG 
GCGTCGGTGA GAGGTGGTAA AACCGTCAGC AATGAAGTCA TTGCCATGAC GGTCAAGGGT 
CCCGGTCTGC AACGCATGGT TCTAGTCGAT TTGCCAGGAA TAATTTCGAC CATGACTGTC 
GACATGGCCT CAGACACAAA AGATTCCATT CACCAGATGA CCAAGCATTA TATGAGCAAT 
CCGAACGCCA TCATTCTCTG CATTCAGGAT GGATCTGTGG ACGCTGAGCG CAGTAATGTG 
ACAGACTTGG TCATGCAGTG CGATCCCTTG GGTCGACGCA CTATTTTTGT GCTCACAAAG 
GTGGATCTGG CCGAGGAGCT CGCCGATCCT GATAGAATAA GGAAAATCCT TTCGGGCAAA 
CTCTTCCCCA TGAAGGCTTT GGGCTACTAT GCCGTCGTTA CCGGTCGTGG ACGAAAGGAT 
GACAGCATAG ATGCTATTCG GCAGTATGAG GAAGACTTCT TTAAGAACTC CAAGCTTTTC 
CATCGTCGAG GGGTTATCAT GCCCCATCAG GTGACCAGCC GCAATCTGAG CTTGGCGGTG 
TCAGATCGTT TCTGGAAGAT GGTGAGGGAA ACCATTGAGC AGCAGGCGGA TGCATTTAAG 
GCGACTAGAT TTAATCTGGA AACCGAATGG AAAAATAACT TCCCCAGATT GCGCGAGTCT 
GGTCGAGATG AGCTGTTCGA CAAGGCAAAG GGCGAGATAC TGGACGAGGT GGTAACGCTC 
TCGCAAATCT CCGCTAAAAA GTGGGACGAC GCTCTGAGCA CCAAGCTATG GGAAAAGCTC 
TCCAATTATG TGTTTGAAAG CATCTATCTG CCCGCTGCAC AGTCAGATTC CTTCAACACG 
ATGGTAGACA TCAAGTTGCG CCAGTGGGCC GAGCAGGCAC TGCCCGCTAA GTCGGTGGAA 
GCTGGCTGGG AAGCGTTGCA GCAGGAATTC ATATCGCTGA TGGAGCGTTC GAAGAAGGCG 
CAGGATCACG ACGGCATCTT CGATCAGTTG AAGTCTGCGG TGGTGGATGA GGCTATACGT 
CGGCACAGCT GGGAGGACAA GGCCATCGAC ATGCTTCGCG TTATCCAGCT GAATACGCTG 
GAGGATCGGT TCGTGCACGA CAAGCAGGAG TGGGATTCGG CGGTTAAGTT CCTGGAGAGT 
TCGGTGAATG CCAAGCTCGT GCAGACGGAG GAGACTTTAG CACAAATGTT TGGTCCCGGC 
CAAATGCGAC GAATTACCCA CTGGCAGTAC CTGACGCAGG ATCAGCAGAA GAGGCGAAGC 
GTTAAGAACG AACTCGACAA AATACTCAAA AACGATACGA AACATTTGCC CACCCTGACC 
CATGATGAAC TGACTACGGT TCGCAAGAAC CTTCAGCGTG ATAACGTGGA TGTGGACACG 
GACTACATAA GGCAAACGTG GTTCCCGGTC TACAGAAAAC ACTTTCTGCA GCAGGCGTTA 
CAGAGGGCAA AGGATTGCCG CAAAGCATAT TACCTCTACA CGCAGCAGGG AGCCGAGTGT 
GAGATATCCT GCAGCGACGT CGTGCTCTTC TGGCGCATCC AGCAGGTGAT CAAGATAACA 
GGCAACGCAC TGAGACAGCA AGTAATCAAT CGGGAGGCGC GGCGGCTGGA CAAGGAGATC 
AAAGCGGTGC TGGACGAGTT CAGCGATGAC GAGGAGAAGA AGGGTTACCT GCTCACCGGC 
AAGCGCGTGC TACTAGCCGA GGAGCTAATC AAAGTGCGGC AGATCCAAGA GAAGCTGGAG 
GAGTTCATCA ATTCACTTAA TCAGGAAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Opa1 ( NM_001299482.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286411.1 Drosophila melanogaster optic atrophy 1, transcript variant C (Opa1), mRNA.

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CloneID	OFa21805
Clone ID Related Accession (Same CDS sequence)	NM_001299482.1
Accession Version	NM_001299482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optic atrophy 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGATCCAAA GCTCAAGCAG 
CTGGGCGAGG ATAAGTTGTC GGAATGGCGC AACTGGTTTG ACAGTCGTCT GGACGATGCC 
ATCGAGGCGG CCGATTATCA AGGAGTTCAG ATTGTCGAAA CCAAAGATGA CTTAAAGGCC 
AAAACAACGG TGGCCGCTTT AGGCATAACA TCCGATGAGA GTCGTAAGAA GTATGAGAAA 
CTCCAGAGCC AGGTGGAGAC GCTGCAGACG GAGATCATGA ACGTCCAGAT TAAGTACCAA 
AAGGAGCTGG AGAAGATGGA GAAGGAGAAC CGTGAACTAC GCCAGCAATA CCTCATCCTG 
AAAACAAACA AAAAGACCAC GGCAAAAAAA ATCAAAAAAT CCCTGATCGA CATGTACTCT 
GAGGTCCTTG ATGAGCTATC CGGCTACGAT ACGGGCTACA CCATGGCCGA TCACCTACCC 
CGTGTTGTGG TAGTGGGAGA TCAGAGCAGC GGCAAGACCT CTGTCCTGGA ATCCATTGCT 
AAGGCTCGCA TCTTTCCCCG TGGCAGTGGA GAGATGATGA CGCGAGCCCC AGTCAAAGTT 
ACTCTGGCTG AAGGACCATA CCATGTGGCT CAGTTCCGTG ACTCTGACCG GGAATACGAT 
CTCACCAAGG AGTCTGATCT GCAGGACCTC CGTCGGGATG TGGAGTTCCG AATGAAGGCG 
TCGGTGAGAG GTGGTAAAAC CGTCAGCAAT GAAGTCATTG CCATGACGGT CAAGGGTCCC 
GGTCTGCAAC GCATGGTTCT AGTCGATTTG CCAGGAATAA TTTCGACCAT GACTGTCGAC 
ATGGCCTCAG ACACAAAAGA TTCCATTCAC CAGATGACCA AGCATTATAT GAGCAATCCG 
AACGCCATCA TTCTCTGCAT TCAGGATGGA TCTGTGGACG CTGAGCGCAG TAATGTGACA 
GACTTGGTCA TGCAGTGCGA TCCCTTGGGT CGACGCACTA TTTTTGTGCT CACAAAGGTG 
GATCTGGCCG AGGAGCTCGC CGATCCTGAT AGAATAAGGA AAATCCTTTC GGGCAAACTC 
TTCCCCATGA AGGCTTTGGG CTACTATGCC GTCGTTACCG GTCGTGGACG AAAGGATGAC 
AGCATAGATG CTATTCGGCA GTATGAGGAA GACTTCTTTA AGAACTCCAA GCTTTTCCAT 
CGTCGAGGGG TTATCATGCC CCATCAGGTG ACCAGCCGCA ATCTGAGCTT GGCGGTGTCA 
GATCGTTTCT GGAAGATGGT GAGGGAAACC ATTGAGCAGC AGGCGGATGC ATTTAAGGCG 
ACTAGATTTA ATCTGGAAAC CGAATGGAAA AATAACTTCC CCAGATTGCG CGAGTCTGGT 
CGAGATGAGC TGTTCGACAA GGCAAAGGGC GAGATACTGG ACGAGGTGGT AACGCTCTCG 
CAAATCTCCG CTAAAAAGTG GGACGACGCT CTGAGCACCA AGCTATGGGA AAAGCTCTCC 
AATTATGTGT TTGAAAGCAT CTATCTGCCC GCTGCACAGT CAGATTCCTT CAACACGATG 
GTAGACATCA AGTTGCGCCA GTGGGCCGAG CAGGCACTGC CCGCTAAGTC GGTGGAAGCT 
GGCTGGGAAG CGTTGCAGCA GGAATTCATA TCGCTGATGG AGCGTTCGAA GAAGGCGCAG 
GATCACGACG GCATCTTCGA TCAGTTGAAG TCTGCGGTGG TGGATGAGGC TATACGTCGG 
CACAGCTGGG AGGACAAGGC CATCGACATG CTTCGCGTTA TCCAGCTGAA TACGCTGGAG 
GATCGGTTCG TGCACGACAA GCAGGAGTGG GATTCGGCGG TTAAGTTCCT GGAGAGTTCG 
GTGAATGCCA AGCTCGTGCA GACGGAGGAG ACTTTAGCAC AAATGTTTGG TCCCGGCCAA 
ATGCGACGAA TTACCCACTG GCAGTACCTG ACGCAGGATC AGCAGAAGAG GCGAAGCGTT 
AAGAACGAAC TCGACAAAAT ACTCAAAAAC GATACGAAAC ATTTGCCCAC CCTGACCCAT 
GATGAACTGA CTACGGTTCG CAAGAACCTT CAGCGTGATA ACGTGGATGT GGACACGGAC 
TACATAAGGC AAACGTGGTT CCCGGTCTAC AGAAAACACT TTCTGCAGCA GGCGTTACAG 
AGGGCAAAGG ATTGCCGCAA AGCATATTAC CTCTACACGC AGCAGGGAGC CGAGTGTGAG 
ATATCCTGCA GCGACGTCGT GCTCTTCTGG CGCATCCAGC AGGTGATCAA GATAACAGGC 
AACGCACTGA GACAGCAAGT AATCAATCGG GAGGCGCGGC GGCTGGACAA GGAGATCAAA 
GCGGTGCTGG ACGAGTTCAG CGATGACGAG GAGAAGAAGG GTTACCTGCT CACCGGCAAG 
CGCGTGCTAC TAGCCGAGGA GCTAATCAAA GTGCGGCAGA TCCAAGAGAA GCTGGAGGAG 
TTCATCAATT CACTTAATCA GGAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286411.1
CDS	136..3045
Translation	MLRIYQNTYRRTARKAVVYSTKVACCNHSTLCGITSHPRRAQDSGSSSSNGRHRGHEEFLLAGNPARGWQMPPPSRGYGMLVVRILRGALKLRYIVLGGAIGGGVSLSKKYEEWKDGLPNFKWLEDAMPQGERWSQFSRNLIEVGSLVKNAIEVDPKLKQLGEDKLSEWRNWFDSRLDDAIEAADYQGVQIVETKDDLKAKTTVAALGITSDESRKKYEKLQSQVETLQTEIMNVQIKYQKELEKMEKENRELRQQYLILKTNKKTTAKKIKKSLIDMYSEVLDELSGYDTGYTMADHLPRVVVVGDQSSGKTSVLESIAKARIFPRGSGEMMTRAPVKVTLAEGPYHVAQFRDSDREYDLTKESDLQDLRRDVEFRMKASVRGGKTVSNEVIAMTVKGPGLQRMVLVDLPGIISTMTVDMASDTKDSIHQMTKHYMSNPNAIILCIQDGSVDAERSNVTDLVMQCDPLGRRTIFVLTKVDLAEELADPDRIRKILSGKLFPMKALGYYAVVTGRGRKDDSIDAIRQYEEDFFKNSKLFHRRGVIMPHQVTSRNLSLAVSDRFWKMVRETIEQQADAFKATRFNLETEWKNNFPRLRESGRDELFDKAKGEILDEVVTLSQISAKKWDDALSTKLWEKLSNYVFESIYLPAAQSDSFNTMVDIKLRQWAEQALPAKSVEAGWEALQQEFISLMERSKKAQDHDGIFDQLKSAVVDEAIRRHSWEDKAIDMLRVIQLNTLEDRFVHDKQEWDSAVKFLESSVNAKLVQTEETLAQMFGPGQMRRITHWQYLTQDQQKRRSVKNELDKILKNDTKHLPTLTHDELTTVRKNLQRDNVDVDTDYIRQTWFPVYRKHFLQQALQRAKDCRKAYYLYTQQGAECEISCSDVVLFWRIQQVIKITGNALRQQVINREARRLDKEIKAVLDEFSDDEEKKGYLLTGKRVLLAEELIKVRQIQEKLEEFINSLNQEK

Target ORF information:

RefSeq Version	NM_001299482.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optic atrophy 1, transcript variant C (Opa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299482.1

ORF Insert Sequence:

ATGTTGCGCA TCTATCAAAA TACTTACCGG CGCACCGCGA GAAAAGCTGT TGTCTACTCC 
ACCAAGGTCG CCTGCTGCAA TCATTCCACG CTCTGTGGCA TCACCAGCCA TCCACGTCGG 
GCGCAGGATA GCGGGAGCTC CAGTTCGAAT GGCAGGCACC GTGGCCACGA GGAGTTCCTA 
CTTGCCGGCA ATCCGGCGCG GGGCTGGCAA ATGCCTCCGC CGTCGCGTGG TTACGGAATG 
CTGGTGGTGC GCATACTGCG GGGAGCCCTC AAGCTGCGAT ACATCGTCCT GGGCGGCGCC 
ATCGGCGGGG GCGTGTCGCT GAGCAAAAAA TACGAGGAAT GGAAGGATGG ACTGCCGAAT 
TTTAAGTGGC TGGAGGATGC CATGCCGCAG GGCGAACGGT GGAGCCAGTT CTCGCGGAAT 
CTCATTGAGG TGGGCTCGCT GGTGAAGAAC GCCATCGAAG TTGATCCAAA GCTCAAGCAG 
CTGGGCGAGG ATAAGTTGTC GGAATGGCGC AACTGGTTTG ACAGTCGTCT GGACGATGCC 
ATCGAGGCGG CCGATTATCA AGGAGTTCAG ATTGTCGAAA CCAAAGATGA CTTAAAGGCC 
AAAACAACGG TGGCCGCTTT AGGCATAACA TCCGATGAGA GTCGTAAGAA GTATGAGAAA 
CTCCAGAGCC AGGTGGAGAC GCTGCAGACG GAGATCATGA ACGTCCAGAT TAAGTACCAA 
AAGGAGCTGG AGAAGATGGA GAAGGAGAAC CGTGAACTAC GCCAGCAATA CCTCATCCTG 
AAAACAAACA AAAAGACCAC GGCAAAAAAA ATCAAAAAAT CCCTGATCGA CATGTACTCT 
GAGGTCCTTG ATGAGCTATC CGGCTACGAT ACGGGCTACA CCATGGCCGA TCACCTACCC 
CGTGTTGTGG TAGTGGGAGA TCAGAGCAGC GGCAAGACCT CTGTCCTGGA ATCCATTGCT 
AAGGCTCGCA TCTTTCCCCG TGGCAGTGGA GAGATGATGA CGCGAGCCCC AGTCAAAGTT 
ACTCTGGCTG AAGGACCATA CCATGTGGCT CAGTTCCGTG ACTCTGACCG GGAATACGAT 
CTCACCAAGG AGTCTGATCT GCAGGACCTC CGTCGGGATG TGGAGTTCCG AATGAAGGCG 
TCGGTGAGAG GTGGTAAAAC CGTCAGCAAT GAAGTCATTG CCATGACGGT CAAGGGTCCC 
GGTCTGCAAC GCATGGTTCT AGTCGATTTG CCAGGAATAA TTTCGACCAT GACTGTCGAC 
ATGGCCTCAG ACACAAAAGA TTCCATTCAC CAGATGACCA AGCATTATAT GAGCAATCCG 
AACGCCATCA TTCTCTGCAT TCAGGATGGA TCTGTGGACG CTGAGCGCAG TAATGTGACA 
GACTTGGTCA TGCAGTGCGA TCCCTTGGGT CGACGCACTA TTTTTGTGCT CACAAAGGTG 
GATCTGGCCG AGGAGCTCGC CGATCCTGAT AGAATAAGGA AAATCCTTTC GGGCAAACTC 
TTCCCCATGA AGGCTTTGGG CTACTATGCC GTCGTTACCG GTCGTGGACG AAAGGATGAC 
AGCATAGATG CTATTCGGCA GTATGAGGAA GACTTCTTTA AGAACTCCAA GCTTTTCCAT 
CGTCGAGGGG TTATCATGCC CCATCAGGTG ACCAGCCGCA ATCTGAGCTT GGCGGTGTCA 
GATCGTTTCT GGAAGATGGT GAGGGAAACC ATTGAGCAGC AGGCGGATGC ATTTAAGGCG 
ACTAGATTTA ATCTGGAAAC CGAATGGAAA AATAACTTCC CCAGATTGCG CGAGTCTGGT 
CGAGATGAGC TGTTCGACAA GGCAAAGGGC GAGATACTGG ACGAGGTGGT AACGCTCTCG 
CAAATCTCCG CTAAAAAGTG GGACGACGCT CTGAGCACCA AGCTATGGGA AAAGCTCTCC 
AATTATGTGT TTGAAAGCAT CTATCTGCCC GCTGCACAGT CAGATTCCTT CAACACGATG 
GTAGACATCA AGTTGCGCCA GTGGGCCGAG CAGGCACTGC CCGCTAAGTC GGTGGAAGCT 
GGCTGGGAAG CGTTGCAGCA GGAATTCATA TCGCTGATGG AGCGTTCGAA GAAGGCGCAG 
GATCACGACG GCATCTTCGA TCAGTTGAAG TCTGCGGTGG TGGATGAGGC TATACGTCGG 
CACAGCTGGG AGGACAAGGC CATCGACATG CTTCGCGTTA TCCAGCTGAA TACGCTGGAG 
GATCGGTTCG TGCACGACAA GCAGGAGTGG GATTCGGCGG TTAAGTTCCT GGAGAGTTCG 
GTGAATGCCA AGCTCGTGCA GACGGAGGAG ACTTTAGCAC AAATGTTTGG TCCCGGCCAA 
ATGCGACGAA TTACCCACTG GCAGTACCTG ACGCAGGATC AGCAGAAGAG GCGAAGCGTT 
AAGAACGAAC TCGACAAAAT ACTCAAAAAC GATACGAAAC ATTTGCCCAC CCTGACCCAT 
GATGAACTGA CTACGGTTCG CAAGAACCTT CAGCGTGATA ACGTGGATGT GGACACGGAC 
TACATAAGGC AAACGTGGTT CCCGGTCTAC AGAAAACACT TTCTGCAGCA GGCGTTACAG 
AGGGCAAAGG ATTGCCGCAA AGCATATTAC CTCTACACGC AGCAGGGAGC CGAGTGTGAG 
ATATCCTGCA GCGACGTCGT GCTCTTCTGG CGCATCCAGC AGGTGATCAA GATAACAGGC 
AACGCACTGA GACAGCAAGT AATCAATCGG GAGGCGCGGC GGCTGGACAA GGAGATCAAA 
GCGGTGCTGG ACGAGTTCAG CGATGACGAG GAGAAGAAGG GTTACCTGCT CACCGGCAAG 
CGCGTGCTAC TAGCCGAGGA GCTAATCAAA GTGCGGCAGA TCCAAGAGAA GCTGGAGGAG 
TTCATCAATT CACTTAATCA GGAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Opa1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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