dgt5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgt5
Entrez Gene ID	36364
Full Name	dim gamma-tubulin 5
Synonyms	CG8828,Dgt5,Dmel\CG8828,msd2
General protein information	Preferred Names dim gamma-tubulin 5 Names CG8828 gene product from transcript CG8828-RA CG8828-PA CG8828-PB dgt5-PA dgt5-PB mitotic spindle density 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49A7-49A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299420.1	NP_001286349.1	dim gamma-tubulin 5, isoform B
NM_136941.3	NP_610785.1	dim gamma-tubulin 5, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005732	XP_315747.3
Drosophila melanogaster (fruit fly)	dgt5	NP_610785.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin (Dgt2-6) and gamma-tubulin function cooperatively to amplify the number of microtubules.
Title: Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.

The following dgt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09757	NM_001299420.1 Latest version!	Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09757	NM_136941.3 Latest version!	Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgt5 ( NM_001299420.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286349.1 Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09757
Clone ID Related Accession (Same CDS sequence)	NM_001299420.1 , NM_136941.3
Accession Version	NM_001299420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dim gamma-tubulin 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATACC AGGAGAAAAT AACAGAATTT AAGAACTGGG CCACTAATCT TGGCTGCCCG 
CCCACTGCCC TGCCCACCGA TGATGCACTC CGAAGAATTT TTAAGTCCGG CTCCAGTTTA 
CTTTTGAACC AGCTGCAATC CCGCATCCAG CCGGTGGATT ACGTGCGGGA GGTGCGCGAG 
AACCTGCTGA TTGCCCAAGT GGCTCGCTAC AAGGACAAAA TGGTGCCGCT GGCCTCTAGG 
AGTTTCCAGC CACCGGAGCT CCAGAGGTAC CAAAAAATAC AGGAGCTAAA GAAGCACAAG 
GAGAAGGCGA ACCAACAGCT TACGGAGGCC CGAAAAGAGT ACCAAAAGCT ATCTGCCGGC 
ATCAAGACCA AGAACATTCA AACCATCAGT GCTGAGAACC GGAAGCAACT ACTTGAATCC 
AAATGCAATA TCCTTGACCT GAAGCTGGAG TCGCTGAATA AGAACTACGA CCAGGAGTTG 
AAGAACAAGA TCCAAATCTT GGGCACCACG CCCGTGAAGC TGAGTGCTAG AAATGCCAGC 
GAGGCGCAGG CAACAAGGGC TGTGGAGCAG GCTCTCAAGC AGCTGGAGAC CTTCTATGGA 
ATGTGTGACG ATGGCAATGC GGTGAACAAT TTAGCCGAGG CCAAGCAGCG ATTATGGGAT 
CAGATGCGGA GCACCTTTGC GGATATTCCC AACTCCCTGC TTCTGAACGT GGTAATGAAG 
ATCAAAGAGG AGCAGCTGCA GCACATCATG AAGCTGAATG AATCCAGAGG CGAATGCACA 
AATGATAAGC CACCGCTGAA TAACTATGAG GTCAAGTTAC TAAAGACCAA GGCCGATATG 
CTGGGTCTGG CGGCCAAATA TTTCGCGGCC CAAAAGGAGC TGGAACTGAA GGAGGAACGC 
TTTTGCCAAG ACTACAGCGT CTTTGTGGAC AAACTGCAAT CAAAGGTATA CAGATTTAAT 
GGCATAAGTT TGGGCGACGA GGAAAATGCG GATGAACTGA TTAGCGACTA TCTGGTGCAG 
TACAACATGC GCAATTTTAA TCGCTCCCAA AACGAATTCC TTCGTGAGCA GATTGAGCAA 
CTGCGTCTGG AGCTAGATGC GGGCGCCAAG CAGCTGGAGA ATCACGATCT AAAGCTAGGC 
TCTGTGAAAC AAGTATACGG CGACATTAAC TCCAGCATCA ATCGCATCCA GCAAGATATG 
GTGCAGCTGT CACAGATCAA GGAGAAGATC CTGTTTTCGC GAAATATGAT GAAGAACCTG 
CTGGACGATA TGCAAGCGGC CACACAGAAA CAAAACGCCA AGTCCCAGCT GATGAGCACT 
AAGCTAAAAG TCAGCAATAT GTCCATGTTG GGTGCGGAGA GTTTTTGCCT GGCCAATGAT 
AGTGTGTTCT CCAGCACCAA GGTGGAGTTC GATGGCAACT GCAGCGCAAT AAACTCAACG 
ATGCGTCGCA GTTTTGACAA CAAAACGCTA GTGCCGGGCG GAGCAGCATC TACCACACTG 
ATGGCTGCAT CCGGAGCCAC GCTACCATCG CATTTGCTGG AATTCAATAC ATTTTTGGAA 
ATACCGCTGG AAAAATTTAG CTGTACGCCG CGCGCCTGCT CCTTCCTGTT GTCGGCCAAT 
CCACTCATCG TCGAGGCCCA GGAGTTGGCC TCCACCGTTC AACTGGCACC AGGCTATTTG 
CTAACACCCT TCGGCGCTCT GCAGGAAGTC CGAAAACGCA TTTTGTGGGC GTCAGCAATT 
GCCGCACACA CGTCTGAGTT GAAATTGAAT TTGCAGCCGT TAATTGTTGA TCCGCACGAT 
TTGAGACTGA AGGCGAGTCG TCAGCACGAG GAAATTGACC AATTGCTGGA CAACCTGATG 
GCCATTGGGG TGAAGACTCA GTTGCAGCTG GAAAAGGCCG AGCGCATCTA TCAGTTCCTG 
CTTGAGAATC CATTGCGACG CTACGTTCCA CCCAGTAAAC GATACAACAA TGGCAGTTTC 
GCGGACTACG AGTCCGAATT CAATCTCTAC TATCGCATGA CCACCAATGG AAGTTCGATG 
CGAGCGCCAC CTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286349.1
CDS	81..2138
Translation	MAYQEKITEFKNWATNLGCPPTALPTDDALRRIFKSGSSLLLNQLQSRIQPVDYVREVRENLLIAQVARYKDKMVPLASRSFQPPELQRYQKIQELKKHKEKANQQLTEARKEYQKLSAGIKTKNIQTISAENRKQLLESKCNILDLKLESLNKNYDQELKNKIQILGTTPVKLSARNASEAQATRAVEQALKQLETFYGMCDDGNAVNNLAEAKQRLWDQMRSTFADIPNSLLLNVVMKIKEEQLQHIMKLNESRGECTNDKPPLNNYEVKLLKTKADMLGLAAKYFAAQKELELKEERFCQDYSVFVDKLQSKVYRFNGISLGDEENADELISDYLVQYNMRNFNRSQNEFLREQIEQLRLELDAGAKQLENHDLKLGSVKQVYGDINSSINRIQQDMVQLSQIKEKILFSRNMMKNLLDDMQAATQKQNAKSQLMSTKLKVSNMSMLGAESFCLANDSVFSSTKVEFDGNCSAINSTMRRSFDNKTLVPGGAASTTLMAASGATLPSHLLEFNTFLEIPLEKFSCTPRACSFLLSANPLIVEAQELASTVQLAPGYLLTPFGALQEVRKRILWASAIAAHTSELKLNLQPLIVDPHDLRLKASRQHEEIDQLLDNLMAIGVKTQLQLEKAERIYQFLLENPLRRYVPPSKRYNNGSFADYESEFNLYYRMTTNGSSMRAPPN

Target ORF information:

RefSeq Version	NM_001299420.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299420.1

ORF Insert Sequence:

ATGGCATACC AGGAGAAAAT AACAGAATTT AAGAACTGGG CCACTAATCT TGGCTGCCCG 
CCCACTGCCC TGCCCACCGA TGATGCACTC CGAAGAATTT TTAAGTCCGG CTCCAGTTTA 
CTTTTGAACC AGCTGCAATC CCGCATCCAG CCGGTGGATT ACGTGCGGGA GGTGCGCGAG 
AACCTGCTGA TTGCCCAAGT GGCTCGCTAC AAGGACAAAA TGGTGCCGCT GGCCTCTAGG 
AGTTTCCAGC CACCGGAGCT CCAGAGGTAC CAAAAAATAC AGGAGCTAAA GAAGCACAAG 
GAGAAGGCGA ACCAACAGCT TACGGAGGCC CGAAAAGAGT ACCAAAAGCT ATCTGCCGGC 
ATCAAGACCA AGAACATTCA AACCATCAGT GCTGAGAACC GGAAGCAACT ACTTGAATCC 
AAATGCAATA TCCTTGACCT GAAGCTGGAG TCGCTGAATA AGAACTACGA CCAGGAGTTG 
AAGAACAAGA TCCAAATCTT GGGCACCACG CCCGTGAAGC TGAGTGCTAG AAATGCCAGC 
GAGGCGCAGG CAACAAGGGC TGTGGAGCAG GCTCTCAAGC AGCTGGAGAC CTTCTATGGA 
ATGTGTGACG ATGGCAATGC GGTGAACAAT TTAGCCGAGG CCAAGCAGCG ATTATGGGAT 
CAGATGCGGA GCACCTTTGC GGATATTCCC AACTCCCTGC TTCTGAACGT GGTAATGAAG 
ATCAAAGAGG AGCAGCTGCA GCACATCATG AAGCTGAATG AATCCAGAGG CGAATGCACA 
AATGATAAGC CACCGCTGAA TAACTATGAG GTCAAGTTAC TAAAGACCAA GGCCGATATG 
CTGGGTCTGG CGGCCAAATA TTTCGCGGCC CAAAAGGAGC TGGAACTGAA GGAGGAACGC 
TTTTGCCAAG ACTACAGCGT CTTTGTGGAC AAACTGCAAT CAAAGGTATA CAGATTTAAT 
GGCATAAGTT TGGGCGACGA GGAAAATGCG GATGAACTGA TTAGCGACTA TCTGGTGCAG 
TACAACATGC GCAATTTTAA TCGCTCCCAA AACGAATTCC TTCGTGAGCA GATTGAGCAA 
CTGCGTCTGG AGCTAGATGC GGGCGCCAAG CAGCTGGAGA ATCACGATCT AAAGCTAGGC 
TCTGTGAAAC AAGTATACGG CGACATTAAC TCCAGCATCA ATCGCATCCA GCAAGATATG 
GTGCAGCTGT CACAGATCAA GGAGAAGATC CTGTTTTCGC GAAATATGAT GAAGAACCTG 
CTGGACGATA TGCAAGCGGC CACACAGAAA CAAAACGCCA AGTCCCAGCT GATGAGCACT 
AAGCTAAAAG TCAGCAATAT GTCCATGTTG GGTGCGGAGA GTTTTTGCCT GGCCAATGAT 
AGTGTGTTCT CCAGCACCAA GGTGGAGTTC GATGGCAACT GCAGCGCAAT AAACTCAACG 
ATGCGTCGCA GTTTTGACAA CAAAACGCTA GTGCCGGGCG GAGCAGCATC TACCACACTG 
ATGGCTGCAT CCGGAGCCAC GCTACCATCG CATTTGCTGG AATTCAATAC ATTTTTGGAA 
ATACCGCTGG AAAAATTTAG CTGTACGCCG CGCGCCTGCT CCTTCCTGTT GTCGGCCAAT 
CCACTCATCG TCGAGGCCCA GGAGTTGGCC TCCACCGTTC AACTGGCACC AGGCTATTTG 
CTAACACCCT TCGGCGCTCT GCAGGAAGTC CGAAAACGCA TTTTGTGGGC GTCAGCAATT 
GCCGCACACA CGTCTGAGTT GAAATTGAAT TTGCAGCCGT TAATTGTTGA TCCGCACGAT 
TTGAGACTGA AGGCGAGTCG TCAGCACGAG GAAATTGACC AATTGCTGGA CAACCTGATG 
GCCATTGGGG TGAAGACTCA GTTGCAGCTG GAAAAGGCCG AGCGCATCTA TCAGTTCCTG 
CTTGAGAATC CATTGCGACG CTACGTTCCA CCCAGTAAAC GATACAACAA TGGCAGTTTC 
GCGGACTACG AGTCCGAATT CAATCTCTAC TATCGCATGA CCACCAATGG AAGTTCGATG 
CGAGCGCCAC CTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgt5 ( NM_136941.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610785.1 Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09757
Clone ID Related Accession (Same CDS sequence)	NM_001299420.1 , NM_136941.3
Accession Version	NM_136941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dim gamma-tubulin 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136941.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATACC AGGAGAAAAT AACAGAATTT AAGAACTGGG CCACTAATCT TGGCTGCCCG 
CCCACTGCCC TGCCCACCGA TGATGCACTC CGAAGAATTT TTAAGTCCGG CTCCAGTTTA 
CTTTTGAACC AGCTGCAATC CCGCATCCAG CCGGTGGATT ACGTGCGGGA GGTGCGCGAG 
AACCTGCTGA TTGCCCAAGT GGCTCGCTAC AAGGACAAAA TGGTGCCGCT GGCCTCTAGG 
AGTTTCCAGC CACCGGAGCT CCAGAGGTAC CAAAAAATAC AGGAGCTAAA GAAGCACAAG 
GAGAAGGCGA ACCAACAGCT TACGGAGGCC CGAAAAGAGT ACCAAAAGCT ATCTGCCGGC 
ATCAAGACCA AGAACATTCA AACCATCAGT GCTGAGAACC GGAAGCAACT ACTTGAATCC 
AAATGCAATA TCCTTGACCT GAAGCTGGAG TCGCTGAATA AGAACTACGA CCAGGAGTTG 
AAGAACAAGA TCCAAATCTT GGGCACCACG CCCGTGAAGC TGAGTGCTAG AAATGCCAGC 
GAGGCGCAGG CAACAAGGGC TGTGGAGCAG GCTCTCAAGC AGCTGGAGAC CTTCTATGGA 
ATGTGTGACG ATGGCAATGC GGTGAACAAT TTAGCCGAGG CCAAGCAGCG ATTATGGGAT 
CAGATGCGGA GCACCTTTGC GGATATTCCC AACTCCCTGC TTCTGAACGT GGTAATGAAG 
ATCAAAGAGG AGCAGCTGCA GCACATCATG AAGCTGAATG AATCCAGAGG CGAATGCACA 
AATGATAAGC CACCGCTGAA TAACTATGAG GTCAAGTTAC TAAAGACCAA GGCCGATATG 
CTGGGTCTGG CGGCCAAATA TTTCGCGGCC CAAAAGGAGC TGGAACTGAA GGAGGAACGC 
TTTTGCCAAG ACTACAGCGT CTTTGTGGAC AAACTGCAAT CAAAGGTATA CAGATTTAAT 
GGCATAAGTT TGGGCGACGA GGAAAATGCG GATGAACTGA TTAGCGACTA TCTGGTGCAG 
TACAACATGC GCAATTTTAA TCGCTCCCAA AACGAATTCC TTCGTGAGCA GATTGAGCAA 
CTGCGTCTGG AGCTAGATGC GGGCGCCAAG CAGCTGGAGA ATCACGATCT AAAGCTAGGC 
TCTGTGAAAC AAGTATACGG CGACATTAAC TCCAGCATCA ATCGCATCCA GCAAGATATG 
GTGCAGCTGT CACAGATCAA GGAGAAGATC CTGTTTTCGC GAAATATGAT GAAGAACCTG 
CTGGACGATA TGCAAGCGGC CACACAGAAA CAAAACGCCA AGTCCCAGCT GATGAGCACT 
AAGCTAAAAG TCAGCAATAT GTCCATGTTG GGTGCGGAGA GTTTTTGCCT GGCCAATGAT 
AGTGTGTTCT CCAGCACCAA GGTGGAGTTC GATGGCAACT GCAGCGCAAT AAACTCAACG 
ATGCGTCGCA GTTTTGACAA CAAAACGCTA GTGCCGGGCG GAGCAGCATC TACCACACTG 
ATGGCTGCAT CCGGAGCCAC GCTACCATCG CATTTGCTGG AATTCAATAC ATTTTTGGAA 
ATACCGCTGG AAAAATTTAG CTGTACGCCG CGCGCCTGCT CCTTCCTGTT GTCGGCCAAT 
CCACTCATCG TCGAGGCCCA GGAGTTGGCC TCCACCGTTC AACTGGCACC AGGCTATTTG 
CTAACACCCT TCGGCGCTCT GCAGGAAGTC CGAAAACGCA TTTTGTGGGC GTCAGCAATT 
GCCGCACACA CGTCTGAGTT GAAATTGAAT TTGCAGCCGT TAATTGTTGA TCCGCACGAT 
TTGAGACTGA AGGCGAGTCG TCAGCACGAG GAAATTGACC AATTGCTGGA CAACCTGATG 
GCCATTGGGG TGAAGACTCA GTTGCAGCTG GAAAAGGCCG AGCGCATCTA TCAGTTCCTG 
CTTGAGAATC CATTGCGACG CTACGTTCCA CCCAGTAAAC GATACAACAA TGGCAGTTTC 
GCGGACTACG AGTCCGAATT CAATCTCTAC TATCGCATGA CCACCAATGG AAGTTCGATG 
CGAGCGCCAC CTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610785.1
CDS	168..2225
Translation	MAYQEKITEFKNWATNLGCPPTALPTDDALRRIFKSGSSLLLNQLQSRIQPVDYVREVRENLLIAQVARYKDKMVPLASRSFQPPELQRYQKIQELKKHKEKANQQLTEARKEYQKLSAGIKTKNIQTISAENRKQLLESKCNILDLKLESLNKNYDQELKNKIQILGTTPVKLSARNASEAQATRAVEQALKQLETFYGMCDDGNAVNNLAEAKQRLWDQMRSTFADIPNSLLLNVVMKIKEEQLQHIMKLNESRGECTNDKPPLNNYEVKLLKTKADMLGLAAKYFAAQKELELKEERFCQDYSVFVDKLQSKVYRFNGISLGDEENADELISDYLVQYNMRNFNRSQNEFLREQIEQLRLELDAGAKQLENHDLKLGSVKQVYGDINSSINRIQQDMVQLSQIKEKILFSRNMMKNLLDDMQAATQKQNAKSQLMSTKLKVSNMSMLGAESFCLANDSVFSSTKVEFDGNCSAINSTMRRSFDNKTLVPGGAASTTLMAASGATLPSHLLEFNTFLEIPLEKFSCTPRACSFLLSANPLIVEAQELASTVQLAPGYLLTPFGALQEVRKRILWASAIAAHTSELKLNLQPLIVDPHDLRLKASRQHEEIDQLLDNLMAIGVKTQLQLEKAERIYQFLLENPLRRYVPPSKRYNNGSFADYESEFNLYYRMTTNGSSMRAPPN

Target ORF information:

RefSeq Version	NM_136941.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dim gamma-tubulin 5 (dgt5), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136941.3

ORF Insert Sequence:

ATGGCATACC AGGAGAAAAT AACAGAATTT AAGAACTGGG CCACTAATCT TGGCTGCCCG 
CCCACTGCCC TGCCCACCGA TGATGCACTC CGAAGAATTT TTAAGTCCGG CTCCAGTTTA 
CTTTTGAACC AGCTGCAATC CCGCATCCAG CCGGTGGATT ACGTGCGGGA GGTGCGCGAG 
AACCTGCTGA TTGCCCAAGT GGCTCGCTAC AAGGACAAAA TGGTGCCGCT GGCCTCTAGG 
AGTTTCCAGC CACCGGAGCT CCAGAGGTAC CAAAAAATAC AGGAGCTAAA GAAGCACAAG 
GAGAAGGCGA ACCAACAGCT TACGGAGGCC CGAAAAGAGT ACCAAAAGCT ATCTGCCGGC 
ATCAAGACCA AGAACATTCA AACCATCAGT GCTGAGAACC GGAAGCAACT ACTTGAATCC 
AAATGCAATA TCCTTGACCT GAAGCTGGAG TCGCTGAATA AGAACTACGA CCAGGAGTTG 
AAGAACAAGA TCCAAATCTT GGGCACCACG CCCGTGAAGC TGAGTGCTAG AAATGCCAGC 
GAGGCGCAGG CAACAAGGGC TGTGGAGCAG GCTCTCAAGC AGCTGGAGAC CTTCTATGGA 
ATGTGTGACG ATGGCAATGC GGTGAACAAT TTAGCCGAGG CCAAGCAGCG ATTATGGGAT 
CAGATGCGGA GCACCTTTGC GGATATTCCC AACTCCCTGC TTCTGAACGT GGTAATGAAG 
ATCAAAGAGG AGCAGCTGCA GCACATCATG AAGCTGAATG AATCCAGAGG CGAATGCACA 
AATGATAAGC CACCGCTGAA TAACTATGAG GTCAAGTTAC TAAAGACCAA GGCCGATATG 
CTGGGTCTGG CGGCCAAATA TTTCGCGGCC CAAAAGGAGC TGGAACTGAA GGAGGAACGC 
TTTTGCCAAG ACTACAGCGT CTTTGTGGAC AAACTGCAAT CAAAGGTATA CAGATTTAAT 
GGCATAAGTT TGGGCGACGA GGAAAATGCG GATGAACTGA TTAGCGACTA TCTGGTGCAG 
TACAACATGC GCAATTTTAA TCGCTCCCAA AACGAATTCC TTCGTGAGCA GATTGAGCAA 
CTGCGTCTGG AGCTAGATGC GGGCGCCAAG CAGCTGGAGA ATCACGATCT AAAGCTAGGC 
TCTGTGAAAC AAGTATACGG CGACATTAAC TCCAGCATCA ATCGCATCCA GCAAGATATG 
GTGCAGCTGT CACAGATCAA GGAGAAGATC CTGTTTTCGC GAAATATGAT GAAGAACCTG 
CTGGACGATA TGCAAGCGGC CACACAGAAA CAAAACGCCA AGTCCCAGCT GATGAGCACT 
AAGCTAAAAG TCAGCAATAT GTCCATGTTG GGTGCGGAGA GTTTTTGCCT GGCCAATGAT 
AGTGTGTTCT CCAGCACCAA GGTGGAGTTC GATGGCAACT GCAGCGCAAT AAACTCAACG 
ATGCGTCGCA GTTTTGACAA CAAAACGCTA GTGCCGGGCG GAGCAGCATC TACCACACTG 
ATGGCTGCAT CCGGAGCCAC GCTACCATCG CATTTGCTGG AATTCAATAC ATTTTTGGAA 
ATACCGCTGG AAAAATTTAG CTGTACGCCG CGCGCCTGCT CCTTCCTGTT GTCGGCCAAT 
CCACTCATCG TCGAGGCCCA GGAGTTGGCC TCCACCGTTC AACTGGCACC AGGCTATTTG 
CTAACACCCT TCGGCGCTCT GCAGGAAGTC CGAAAACGCA TTTTGTGGGC GTCAGCAATT 
GCCGCACACA CGTCTGAGTT GAAATTGAAT TTGCAGCCGT TAATTGTTGA TCCGCACGAT 
TTGAGACTGA AGGCGAGTCG TCAGCACGAG GAAATTGACC AATTGCTGGA CAACCTGATG 
GCCATTGGGG TGAAGACTCA GTTGCAGCTG GAAAAGGCCG AGCGCATCTA TCAGTTCCTG 
CTTGAGAATC CATTGCGACG CTACGTTCCA CCCAGTAAAC GATACAACAA TGGCAGTTTC 
GCGGACTACG AGTCCGAATT CAATCTCTAC TATCGCATGA CCACCAATGG AAGTTCGATG 
CGAGCGCCAC CTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dgt5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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