ths cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ths
Entrez Gene ID	36258
Full Name	thisbe
Synonyms	CG12443,DFGF8-1,DmThs,Dmel\CG12443,FGF8-like1,Neu4,Ths,fgf8-like
General protein information	Preferred Names thisbe Names CG12443 gene product from transcript CG12443-RB CG12443-PA CG12443-PB ths-PA ths-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C3-48C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259339.2	NP_001246268.1	thisbe, isoform B
NM_136857.4	NP_610701.2	thisbe, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Proliferation of epithelial sheath progenitors, the apical cells, was decreased in both htl and ths mutants, while ectopic expression of the Ths ligand led to these cells' over-proliferation suggesting that FGF signaling supports ovarian muscle sheath formation by controlling apical cell number in the developing gonad.
Title: FGF signaling supports Drosophila fertility by regulating development of ovarian muscle tissues.

Data indicate that Pyramus (Pyr) and Thisbe (Ths) signals play an obligatory role for the survival of the migrating cells.
Title: The FGF8-related signals Pyramus and Thisbe promote pathfinding, substrate adhesion, and survival of migrating longitudinal gut muscle founder cells.

Data show that FGF signaling regulates directional movement of caudal visceral mesoderm cells and that presentation of both FGF ligands, Pyramus and Thisbe, together is most effective at attracting cells.
Title: Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands.

Ths and Pyr proteins are secreted from S2 cells as N-terminal fragments as a result of intracellular proteolytic cleavage; the FGF-domain is contained within the secreted ligand portion, and is functions in the embryo when ectopically expressed.
Title: Analysis of Thisbe and Pyramus functional domains reveals evidence for cleavage of Drosophila FGFs.

pyr and ths single mutants display defects in gastrulation and mesoderm differentiation.
Title: Differential and overlapping functions of two closely related Drosophila FGF8-like growth factors in mesoderm development.

The following ths gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ths cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09642	NM_136857.4 Latest version!	Drosophila melanogaster thisbe (ths), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa13283	NM_001259339.2 Latest version!	Drosophila melanogaster thisbe (ths), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ths ( NM_136857.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610701.2 Drosophila melanogaster thisbe (ths), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09642
Clone ID Related Accession (Same CDS sequence)	NM_136857.4
Accession Version	NM_136857.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thisbe, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136857.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAATC AGTTAGAGAG ACTGCTGTTT TTTATTGTGG TGATTTCAGG TGCATTATGT 
ACAGTAGAAG ATTACGTAAT AATGTCACAG TGTGCACACA ACAAAGCCAT ACACATAGCT 
GCGGAAGGAA CAGTCTCAGT GACAGACACT TCACAGATAC AAAATATAAC GATAGTCGGC 
TTCCCGGATT ATCTGAACAA CGAATTCAAA ATAGCACTGT ACGCAAAAGA AACTCGAAGA 
TATTTGTGTT TCAACGACAA TTGGAGATTA GTTGGCATGA GGGAGCTGCG AGATACCTGC 
TATTTCAACG AGACCATCGT GCACGGATAT TTCGTGTTCC GTTCCGTTGT CGATCTGCAG 
CGACGTGTCG GGTTCACGCA CCGAGGTAAG CCTGTGGGGC CCAAGAAGAG CGTCAACGAT 
GCCTGCTACA TGTTCAACAA GATCGAGGCC GAGCAGTTTT TCCGCCATCA CCATAACAAT 
GGCAACAGTG GCAACAACAA TGGCAATGGC AGTGGCAATG ACAATGGCAA TGGCAGCAAC 
AACAGTCGCA AGCCCCCGCG CAACAACAAA AACAATCGCC ACAAATCGAA TAACCAAAAT 
CAAAAGTTGC AACAGCAACA GGAGCATCAT GGCCATAATA ATAATAATAA TAACGTTATT 
CTTAATAATA GTAATACTAG TTTAAGTAGC ACTAACAAAA AGCAATTAGC GATAAACAGG 
CAGAGGCAGC AACAGCAGCA ACATCAGGTG CGACATCATC ACAATGATCC ATCGCTGCTG 
CTGCGACGAC AGCAACACGA GAAGAAGCAG AAGCGGCGCA AGCAACAGAA GCAGCAGCAA 
CACCAACAAC GAGTATCAGC TAGTCCAACG AATCCGATGC CTGCAACACC CACAGCAGCG 
CCAGCAGCAA CACCAACCGT TGCAGCCACA TCGCCGGCAA CAGCAACACG ATCGACAACA 
GCAGCAATGA GCAGCAAGCG CAAGGGCCGG CGAAGAAAGG CCAAGGTCAG GCCAAAGGCG 
CAACAGCAAC AACAGTTGCA GCTACTTGCC ACGGCAGCAA CATCGCCCTA CACCGACGAC 
ACCCTGTACG GCAGCAGCAG TAGCAGCAGC AGCACGGGTT TCGAGGATTT GGACATCTAC 
AATAGCAGCA GCAGCAGCGG CAGCAGTTCC AGCTTGGATC CCTGGTCCAC TTGGTCCACG 
ATCGATAGCT TTAGCGGCAG CAGCACAACA GCAACATCTG ACGACAGTAT TAGCAGCAGC 
AGCAGCAGCA ACTACGACGC CTGGGCCACC TACATCACCG AAATGGAGGC CGAGGCATCG 
GCCATGACGC CCGAGATGGG CGGCAACGAC ACCGAGTTGA TGCAGTACGA GGCCGAGTTG 
GTGGACGATC CGGAGGCGGA GTCCACGGCG ACAATTGAAA CCAAAGCAAC ATCAGTAACA 
TCAACAATAG CGGCAACGGC GGCCACAACT ACCTCGACAG CGGGCTCCCA GCTGGTGATA 
GAGCAGACGC CGCTGGCCAG GAGCAACATC AGCAACAAGA GCAGCAGCAG CACGAAAGCA 
ACACCAACGG CAACCAGTCA GACTGAAACA GCACAGAGCA CGCAACAACC AAGCAGGCAT 
CGCAGTAACA GCAGGGAAGA TCACCTGGAC AATTCCGACA TGTTGCTAGC GGGCGAGAAC 
GAAGTGAAGC CACAACAGCC ACGCAAGTTA CTGTGGACAG TGCAACCACC GCTCGCAACA 
CGTGGCAGCA CACTAGCAAC ATTGGTGGCA ACACCAGAGC AGCAACACAC CAGCACGACA 
GCAACCGCGG CCACGACACC GGAGCAACAT GTTGCACCGT CACTCTTCAG CGTGGACAAG 
AACATCAACA GCAACCTGAA CAACACACTA ACGGAGGCTT CTCCCACGGC AACATCAACA 
TCCAGCATCA CGTCCGCAAC AACCATGAAG AGACCCATAC GGAAGTTCAC CAAGAAGCTA 
ATGGCCACGC CCTACCACCA GCTGACCTAT GTGCGCAATG CGGGCGGAGA TATCGACATC 
GATAACCTGG ACAACATCAG TGTGTATCCC GTTCTCTACG ATGGTGATCT GGAGGGGAAT 
GGCGGCGGCA ACAGCTCCGA CGGCGGCTTC AGCGGGTTCC TGCCCACCTC GACCACCTCA 
CACCTGACGG AACCGATTCG CATCGGCATG AACAGGATAA GGCCGAATAA GACACTGCAT 
TGGTTCACTC ATCAGAGATT TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610701.2
CDS	534..2780
Translation	MSNQLERLLFFIVVISGALCTVEDYVIMSQCAHNKAIHIAAEGTVSVTDTSQIQNITIVGFPDYLNNEFKIALYAKETRRYLCFNDNWRLVGMRELRDTCYFNETIVHGYFVFRSVVDLQRRVGFTHRGKPVGPKKSVNDACYMFNKIEAEQFFRHHHNNGNSGNNNGNGSGNDNGNGSNNSRKPPRNNKNNRHKSNNQNQKLQQQQEHHGHNNNNNNVILNNSNTSLSSTNKKQLAINRQRQQQQQHQVRHHHNDPSLLLRRQQHEKKQKRRKQQKQQQHQQRVSASPTNPMPATPTAAPAATPTVAATSPATATRSTTAAMSSKRKGRRRKAKVRPKAQQQQQLQLLATAATSPYTDDTLYGSSSSSSSTGFEDLDIYNSSSSSGSSSSLDPWSTWSTIDSFSGSSTTATSDDSISSSSSSNYDAWATYITEMEAEASAMTPEMGGNDTELMQYEAELVDDPEAESTATIETKATSVTSTIAATAATTTSTAGSQLVIEQTPLARSNISNKSSSSTKATPTATSQTETAQSTQQPSRHRSNSREDHLDNSDMLLAGENEVKPQQPRKLLWTVQPPLATRGSTLATLVATPEQQHTSTTATAATTPEQHVAPSLFSVDKNINSNLNNTLTEASPTATSTSSITSATTMKRPIRKFTKKLMATPYHQLTYVRNAGGDIDIDNLDNISVYPVLYDGDLEGNGGGNSSDGGFSGFLPTSTTSHLTEPIRIGMNRIRPNKTLHWFTHQRFA

Target ORF information:

RefSeq Version	NM_136857.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thisbe (ths), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136857.4

ORF Insert Sequence:

ATGTCGAATC AGTTAGAGAG ACTGCTGTTT TTTATTGTGG TGATTTCAGG TGCATTATGT 
ACAGTAGAAG ATTACGTAAT AATGTCACAG TGTGCACACA ACAAAGCCAT ACACATAGCT 
GCGGAAGGAA CAGTCTCAGT GACAGACACT TCACAGATAC AAAATATAAC GATAGTCGGC 
TTCCCGGATT ATCTGAACAA CGAATTCAAA ATAGCACTGT ACGCAAAAGA AACTCGAAGA 
TATTTGTGTT TCAACGACAA TTGGAGATTA GTTGGCATGA GGGAGCTGCG AGATACCTGC 
TATTTCAACG AGACCATCGT GCACGGATAT TTCGTGTTCC GTTCCGTTGT CGATCTGCAG 
CGACGTGTCG GGTTCACGCA CCGAGGTAAG CCTGTGGGGC CCAAGAAGAG CGTCAACGAT 
GCCTGCTACA TGTTCAACAA GATCGAGGCC GAGCAGTTTT TCCGCCATCA CCATAACAAT 
GGCAACAGTG GCAACAACAA TGGCAATGGC AGTGGCAATG ACAATGGCAA TGGCAGCAAC 
AACAGTCGCA AGCCCCCGCG CAACAACAAA AACAATCGCC ACAAATCGAA TAACCAAAAT 
CAAAAGTTGC AACAGCAACA GGAGCATCAT GGCCATAATA ATAATAATAA TAACGTTATT 
CTTAATAATA GTAATACTAG TTTAAGTAGC ACTAACAAAA AGCAATTAGC GATAAACAGG 
CAGAGGCAGC AACAGCAGCA ACATCAGGTG CGACATCATC ACAATGATCC ATCGCTGCTG 
CTGCGACGAC AGCAACACGA GAAGAAGCAG AAGCGGCGCA AGCAACAGAA GCAGCAGCAA 
CACCAACAAC GAGTATCAGC TAGTCCAACG AATCCGATGC CTGCAACACC CACAGCAGCG 
CCAGCAGCAA CACCAACCGT TGCAGCCACA TCGCCGGCAA CAGCAACACG ATCGACAACA 
GCAGCAATGA GCAGCAAGCG CAAGGGCCGG CGAAGAAAGG CCAAGGTCAG GCCAAAGGCG 
CAACAGCAAC AACAGTTGCA GCTACTTGCC ACGGCAGCAA CATCGCCCTA CACCGACGAC 
ACCCTGTACG GCAGCAGCAG TAGCAGCAGC AGCACGGGTT TCGAGGATTT GGACATCTAC 
AATAGCAGCA GCAGCAGCGG CAGCAGTTCC AGCTTGGATC CCTGGTCCAC TTGGTCCACG 
ATCGATAGCT TTAGCGGCAG CAGCACAACA GCAACATCTG ACGACAGTAT TAGCAGCAGC 
AGCAGCAGCA ACTACGACGC CTGGGCCACC TACATCACCG AAATGGAGGC CGAGGCATCG 
GCCATGACGC CCGAGATGGG CGGCAACGAC ACCGAGTTGA TGCAGTACGA GGCCGAGTTG 
GTGGACGATC CGGAGGCGGA GTCCACGGCG ACAATTGAAA CCAAAGCAAC ATCAGTAACA 
TCAACAATAG CGGCAACGGC GGCCACAACT ACCTCGACAG CGGGCTCCCA GCTGGTGATA 
GAGCAGACGC CGCTGGCCAG GAGCAACATC AGCAACAAGA GCAGCAGCAG CACGAAAGCA 
ACACCAACGG CAACCAGTCA GACTGAAACA GCACAGAGCA CGCAACAACC AAGCAGGCAT 
CGCAGTAACA GCAGGGAAGA TCACCTGGAC AATTCCGACA TGTTGCTAGC GGGCGAGAAC 
GAAGTGAAGC CACAACAGCC ACGCAAGTTA CTGTGGACAG TGCAACCACC GCTCGCAACA 
CGTGGCAGCA CACTAGCAAC ATTGGTGGCA ACACCAGAGC AGCAACACAC CAGCACGACA 
GCAACCGCGG CCACGACACC GGAGCAACAT GTTGCACCGT CACTCTTCAG CGTGGACAAG 
AACATCAACA GCAACCTGAA CAACACACTA ACGGAGGCTT CTCCCACGGC AACATCAACA 
TCCAGCATCA CGTCCGCAAC AACCATGAAG AGACCCATAC GGAAGTTCAC CAAGAAGCTA 
ATGGCCACGC CCTACCACCA GCTGACCTAT GTGCGCAATG CGGGCGGAGA TATCGACATC 
GATAACCTGG ACAACATCAG TGTGTATCCC GTTCTCTACG ATGGTGATCT GGAGGGGAAT 
GGCGGCGGCA ACAGCTCCGA CGGCGGCTTC AGCGGGTTCC TGCCCACCTC GACCACCTCA 
CACCTGACGG AACCGATTCG CATCGGCATG AACAGGATAA GGCCGAATAA GACACTGCAT 
TGGTTCACTC ATCAGAGATT TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ths ( NM_001259339.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246268.1 Drosophila melanogaster thisbe (ths), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13283
Clone ID Related Accession (Same CDS sequence)	NM_001259339.2
Accession Version	NM_001259339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thisbe, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259339.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGTGA TTTCAGGTGC ATTATGTACA GTAGAAGATT ACGTAATAAT GTCACAGTGT 
GCACACAACA AAGCCATACA CATAGCTGCG GAAGGAACAG TCTCAGTGAC AGACACTTCA 
CAGATACAAA ATATAACGAT AGTCGGCTTC CCGGATTATC TGAACAACGA ATTCAAAATA 
GCACTGTACG CAAAAGAAAC TCGAAGATAT TTGTGTTTCA ACGACAATTG GAGATTAGTT 
GGCATGAGGG AGCTGCGAGA TACCTGCTAT TTCAACGAGA CCATCGTGCA CGGATATTTC 
GTGTTCCGTT CCGTTGTCGA TCTGCAGCGA CGTGTCGGGT TCACGCACCG AGGTAAGCCT 
GTGGGGCCCA AGAAGAGCGT CAACGATGCC TGCTACATGT TCAACAAGAT CGAGGCCGAG 
CAGTTTTTCC GCCATCACCA TAACAATGGC AACAGTGGCA ACAACAATGG CAATGGCAGT 
GGCAATGACA ATGGCAATGG CAGCAACAAC AGTCGCAAGC CCCCGCGCAA CAACAAAAAC 
AATCGCCACA AATCGAATAA CCAAAATCAA AAGTTGCAAC AGCAACAGGA GCATCATGGC 
CATAATAATA ATAATAATAA CGTTATTCTT AATAATAGTA ATACTAGTTT AAGTAGCACT 
AACAAAAAGC AATTAGCGAT AAACAGGCAG AGGCAGCAAC AGCAGCAACA TCAGGTGCGA 
CATCATCACA ATGATCCATC GCTGCTGCTG CGACGACAGC AACACGAGAA GAAGCAGAAG 
CGGCGCAAGC AACAGAAGCA GCAGCAACAC CAACAACGAG TATCAGCTAG TCCAACGAAT 
CCGATGCCTG CAACACCCAC AGCAGCGCCA GCAGCAACAC CAACCGTTGC AGCCACATCG 
CCGGCAACAG CAACACGATC GACAACAGCA GCAATGAGCA GCAAGCGCAA GGGCCGGCGA 
AGAAAGGCCA AGGTCAGGCC AAAGGCGCAA CAGCAACAAC AGTTGCAGCT ACTTGCCACG 
GCAGCAACAT CGCCCTACAC CGACGACACC CTGTACGGCA GCAGCAGTAG CAGCAGCAGC 
ACGGGTTTCG AGGATTTGGA CATCTACAAT AGCAGCAGCA GCAGCGGCAG CAGTTCCAGC 
TTGGATCCCT GGTCCACTTG GTCCACGATC GATAGCTTTA GCGGCAGCAG CACAACAGCA 
ACATCTGACG ACAGTATTAG CAGCAGCAGC AGCAGCAACT ACGACGCCTG GGCCACCTAC 
ATCACCGAAA TGGAGGCCGA GGCATCGGCC ATGACGCCCG AGATGGGCGG CAACGACACC 
GAGTTGATGC AGTACGAGGC CGAGTTGGTG GACGATCCGG AGGCGGAGTC CACGGCGACA 
ATTGAAACCA AAGCAACATC AGTAACATCA ACAATAGCGG CAACGGCGGC CACAACTACC 
TCGACAGCGG GCTCCCAGCT GGTGATAGAG CAGACGCCGC TGGCCAGGAG CAACATCAGC 
AACAAGAGCA GCAGCAGCAC GAAAGCAACA CCAACGGCAA CCAGTCAGAC TGAAACAGCA 
CAGAGCACGC AACAACCAAG CAGGCATCGC AGTAACAGCA GGGAAGATCA CCTGGACAAT 
TCCGACATGT TGCTAGCGGG CGAGAACGAA GTGAAGCCAC AACAGCCACG CAAGTTACTG 
TGGACAGTGC AACCACCGCT CGCAACACGT GGCAGCACAC TAGCAACATT GGTGGCAACA 
CCAGAGCAGC AACACACCAG CACGACAGCA ACCGCGGCCA CGACACCGGA GCAACATGTT 
GCACCGTCAC TCTTCAGCGT GGACAAGAAC ATCAACAGCA ACCTGAACAA CACACTAACG 
GAGGCTTCTC CCACGGCAAC ATCAACATCC AGCATCACGT CCGCAACAAC CATGAAGAGA 
CCCATACGGA AGTTCACCAA GAAGCTAATG GCCACGCCCT ACCACCAGCT GACCTATGTG 
CGCAATGCGG GCGGAGATAT CGACATCGAT AACCTGGACA ACATCAGTGT GTATCCCGTT 
CTCTACGATG GTGATCTGGA GGGGAATGGC GGCGGCAACA GCTCCGACGG CGGCTTCAGC 
GGGTTCCTGC CCACCTCGAC CACCTCACAC CTGACGGAAC CGATTCGCAT CGGCATGAAC 
AGGATAAGGC CGAATAAGAC ACTGCATTGG TTCACTCATC AGAGATTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246268.1
CDS	2729..4942
Translation	MVVISGALCTVEDYVIMSQCAHNKAIHIAAEGTVSVTDTSQIQNITIVGFPDYLNNEFKIALYAKETRRYLCFNDNWRLVGMRELRDTCYFNETIVHGYFVFRSVVDLQRRVGFTHRGKPVGPKKSVNDACYMFNKIEAEQFFRHHHNNGNSGNNNGNGSGNDNGNGSNNSRKPPRNNKNNRHKSNNQNQKLQQQQEHHGHNNNNNNVILNNSNTSLSSTNKKQLAINRQRQQQQQHQVRHHHNDPSLLLRRQQHEKKQKRRKQQKQQQHQQRVSASPTNPMPATPTAAPAATPTVAATSPATATRSTTAAMSSKRKGRRRKAKVRPKAQQQQQLQLLATAATSPYTDDTLYGSSSSSSSTGFEDLDIYNSSSSSGSSSSLDPWSTWSTIDSFSGSSTTATSDDSISSSSSSNYDAWATYITEMEAEASAMTPEMGGNDTELMQYEAELVDDPEAESTATIETKATSVTSTIAATAATTTSTAGSQLVIEQTPLARSNISNKSSSSTKATPTATSQTETAQSTQQPSRHRSNSREDHLDNSDMLLAGENEVKPQQPRKLLWTVQPPLATRGSTLATLVATPEQQHTSTTATAATTPEQHVAPSLFSVDKNINSNLNNTLTEASPTATSTSSITSATTMKRPIRKFTKKLMATPYHQLTYVRNAGGDIDIDNLDNISVYPVLYDGDLEGNGGGNSSDGGFSGFLPTSTTSHLTEPIRIGMNRIRPNKTLHWFTHQRFA

Target ORF information:

RefSeq Version	NM_001259339.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thisbe (ths), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259339.2

ORF Insert Sequence:

ATGGTGGTGA TTTCAGGTGC ATTATGTACA GTAGAAGATT ACGTAATAAT GTCACAGTGT 
GCACACAACA AAGCCATACA CATAGCTGCG GAAGGAACAG TCTCAGTGAC AGACACTTCA 
CAGATACAAA ATATAACGAT AGTCGGCTTC CCGGATTATC TGAACAACGA ATTCAAAATA 
GCACTGTACG CAAAAGAAAC TCGAAGATAT TTGTGTTTCA ACGACAATTG GAGATTAGTT 
GGCATGAGGG AGCTGCGAGA TACCTGCTAT TTCAACGAGA CCATCGTGCA CGGATATTTC 
GTGTTCCGTT CCGTTGTCGA TCTGCAGCGA CGTGTCGGGT TCACGCACCG AGGTAAGCCT 
GTGGGGCCCA AGAAGAGCGT CAACGATGCC TGCTACATGT TCAACAAGAT CGAGGCCGAG 
CAGTTTTTCC GCCATCACCA TAACAATGGC AACAGTGGCA ACAACAATGG CAATGGCAGT 
GGCAATGACA ATGGCAATGG CAGCAACAAC AGTCGCAAGC CCCCGCGCAA CAACAAAAAC 
AATCGCCACA AATCGAATAA CCAAAATCAA AAGTTGCAAC AGCAACAGGA GCATCATGGC 
CATAATAATA ATAATAATAA CGTTATTCTT AATAATAGTA ATACTAGTTT AAGTAGCACT 
AACAAAAAGC AATTAGCGAT AAACAGGCAG AGGCAGCAAC AGCAGCAACA TCAGGTGCGA 
CATCATCACA ATGATCCATC GCTGCTGCTG CGACGACAGC AACACGAGAA GAAGCAGAAG 
CGGCGCAAGC AACAGAAGCA GCAGCAACAC CAACAACGAG TATCAGCTAG TCCAACGAAT 
CCGATGCCTG CAACACCCAC AGCAGCGCCA GCAGCAACAC CAACCGTTGC AGCCACATCG 
CCGGCAACAG CAACACGATC GACAACAGCA GCAATGAGCA GCAAGCGCAA GGGCCGGCGA 
AGAAAGGCCA AGGTCAGGCC AAAGGCGCAA CAGCAACAAC AGTTGCAGCT ACTTGCCACG 
GCAGCAACAT CGCCCTACAC CGACGACACC CTGTACGGCA GCAGCAGTAG CAGCAGCAGC 
ACGGGTTTCG AGGATTTGGA CATCTACAAT AGCAGCAGCA GCAGCGGCAG CAGTTCCAGC 
TTGGATCCCT GGTCCACTTG GTCCACGATC GATAGCTTTA GCGGCAGCAG CACAACAGCA 
ACATCTGACG ACAGTATTAG CAGCAGCAGC AGCAGCAACT ACGACGCCTG GGCCACCTAC 
ATCACCGAAA TGGAGGCCGA GGCATCGGCC ATGACGCCCG AGATGGGCGG CAACGACACC 
GAGTTGATGC AGTACGAGGC CGAGTTGGTG GACGATCCGG AGGCGGAGTC CACGGCGACA 
ATTGAAACCA AAGCAACATC AGTAACATCA ACAATAGCGG CAACGGCGGC CACAACTACC 
TCGACAGCGG GCTCCCAGCT GGTGATAGAG CAGACGCCGC TGGCCAGGAG CAACATCAGC 
AACAAGAGCA GCAGCAGCAC GAAAGCAACA CCAACGGCAA CCAGTCAGAC TGAAACAGCA 
CAGAGCACGC AACAACCAAG CAGGCATCGC AGTAACAGCA GGGAAGATCA CCTGGACAAT 
TCCGACATGT TGCTAGCGGG CGAGAACGAA GTGAAGCCAC AACAGCCACG CAAGTTACTG 
TGGACAGTGC AACCACCGCT CGCAACACGT GGCAGCACAC TAGCAACATT GGTGGCAACA 
CCAGAGCAGC AACACACCAG CACGACAGCA ACCGCGGCCA CGACACCGGA GCAACATGTT 
GCACCGTCAC TCTTCAGCGT GGACAAGAAC ATCAACAGCA ACCTGAACAA CACACTAACG 
GAGGCTTCTC CCACGGCAAC ATCAACATCC AGCATCACGT CCGCAACAAC CATGAAGAGA 
CCCATACGGA AGTTCACCAA GAAGCTAATG GCCACGCCCT ACCACCAGCT GACCTATGTG 
CGCAATGCGG GCGGAGATAT CGACATCGAT AACCTGGACA ACATCAGTGT GTATCCCGTT 
CTCTACGATG GTGATCTGGA GGGGAATGGC GGCGGCAACA GCTCCGACGG CGGCTTCAGC 
GGGTTCCTGC CCACCTCGAC CACCTCACAC CTGACGGAAC CGATTCGCAT CGGCATGAAC 
AGGATAAGGC CGAATAAGAC ACTGCATTGG TTCACTCATC AGAGATTTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
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Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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ths cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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