en cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	en
Entrez Gene ID	36240
Full Name	engrailed
Synonyms	153867_at,Apa,CG9015,Dmel\CG9015,EN,En,EnR,Eng,Engr,Engrailed,Engrailed/Invected,Es,V,en1,spa2,spt
General protein information	Preferred Names engrailed Names CG9015 gene product from transcript CG9015-RB CG9015-PA CG9015-PB ENGRAILED apigmented abdomen en-PA en-PB erased invected sparse SGPs 2 spermatheca transcript group V
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47F17-48A1\|2-62.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_165841.2	NP_725059.1	engrailed, isoform B
NM_078976.4	NP_523700.2	engrailed, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

En forms a complex with Nejire (Nej), the Drosophila ortholog of histone acetyltransferase CBP/p300, and directs Nej to this cis-regulatory region where Nej functions as the co-activator for dpp expression.
Title: Engrailed acts with Nejire to control

Data indicate there is redundancy in both regulatory DNA and protein function in invected (inv) and engrailed (en) genes.
Title: Co-regulation of invected and engrailed by a complex array of regulatory sequences in Drosophila.

these results suggest that En in Johnston's Organ neurons regulate both neuronal excitability and synaptic connectivity.
Title: Engrailed alters the specificity of synaptic connections of Drosophila auditory neurons with the giant fiber.

This study showed that small subset of FRUM neurons distributed in the brain and ventral cord of males by their co-expression with in En neurons
Title: A small cohort of FRU(M) and Engrailed-expressing neurons mediate successful copulation in Drosophila melanogaster.

Silencing Engrailedin Johnston's organ neurons reduces the sound-evoked potential amplitude at the onset of the stimulus by about half at 100, 200 and 400 Hz.
Title: Auditory responses of engrailed and invected-expressing Johnston's Organ neurons in Drosophila melanogaster.

The following en gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the en cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09618	NM_165841.2 Latest version!	Drosophila melanogaster engrailed (en), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa09618	NM_078976.4 Latest version!	Drosophila melanogaster engrailed (en), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

en ( NM_165841.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725059.1 Drosophila melanogaster engrailed (en), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09618
Clone ID Related Accession (Same CDS sequence)	NM_165841.2 , NM_078976.4
Accession Version	NM_165841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	engrailed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165841.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCTGG AGGATCGCTG CAGTCCACAG TCAGCGCCCA GCCCCATTAC CCTACAAATG 
CAGCATCTTC ACCACCAGCA ACAGCAGCAG CAGCAACAGC AGCAGCAAAT GCAGCACCTC 
CACCAGCTGC AGCAACTGCA GCAGTTGCAC CAACAGCAAC TGGCCGCCGG TGTCTTCCAC 
CATCCGGCAA TGGCCTTCGA TGCCGCTGCA GCCGCCGCTG CTGCAGCTGC TGCTGCGGCC 
GCCCACGCTC ATGCTGCTGC ACTGCAGCAG CGCCTCAGTG GCAGTGGATC GCCCGCATCC 
TGCTCCACGC CCGCCTCGTC CACGCCGCTG ACCATCAAGG AGGAGGAAAG CGACTCCGTG 
ATCGGTGACA TGAGTTTCCA CAATCAGACG CACACCACCA ACGAGGAGGA GGAGGCGGAG 
GAGGATGACG ACATTGATGT GGATGTGGAT GATACGTCGG CGGGCGGACG CCTGCCACCA 
CCCGCCCACC AGCAGCAGTC GACGGCCAAG CCCTCGCTGG CCTTTTCCAT CTCCAACATC 
CTGAGCGATC GTTTCGGAGA TGTCCAGAAG CCGGGCAAGT CGATGGAGAA CCAGGCCAGC 
ATATTCCGCC CCTTCGAGGC GAGTCGTTCC CAGACTGCCA CGCCCTCCGC CTTTACAAGA 
GTGGATCTGC TGGAGTTTAG CCGGCAACAG CAGGCTGCCG CCGCAGCCGC TACTGCGGCC 
ATGATGCTGG AACGGGCCAA CTTCCTTAAC TGCTTCAATC CGGCTGCCTA TCCCAGGATA 
CACGAGGAAA TCGTGCAGAG TCGGCTGCGC AGGAGTGCAG CCAATGCCGT CATCCCGCCG 
CCCATGAGCT CCAAGATGAG CGATGCCAAT CCAGAGAAAT CTGCTCTGGG ATCCCTGTGC 
AAGGCGGTCT CGCAGATCGG ACAACCTGCT GCCCCTACGA TGACCCAACC TCCGCTGAGT 
AGCAGTGCCA GCAGCTTGGC CAGTCCGCCA CCCGCCTCCA ATGCCTCGAC CATTAGCAGC 
ACCTCTTCCG TGGCCACCAG CTCGAGCTCC TCCTCGTCGG GTTGCTCCTC GGCGGCCAGT 
TCCTTGAACT CCTCGCCCAG TAGCCGACTG GGAGCCAGTG GATCCGGAGT CAATGCCAGC 
AGTCCCCAGC CGCAGCCAAT CCCGCCGCCA TCCGCCGTTA GCCGAGATTC CGGAATGGAG 
TCCTCGGATG ACACGCGTTC CGAGACGGGA TCCACCACCA CAGAGGGCGG CAAGAACGAG 
ATGTGGCCCG CCTGGGTGTA CTGCACCCGC TACAGCGATC GTCCCAGCTC AGGACCCCGC 
TACCGCCGCC CCAAACAGCC AAAGGACAAG ACCAACGACG AGAAGCGTCC ACGCACCGCG 
TTCTCCAGCG AGCAGTTGGC CCGCCTTAAG CGGGAGTTCA ACGAGAATCG CTATCTGACC 
GAGCGGAGAC GCCAGCAGCT GAGCAGCGAG TTGGGCCTGA ACGAGGCGCA GATCAAGATC 
TGGTTCCAGA ACAAGCGGGC CAAGATCAAG AAGTCGACGG GCTCCAAAAA TCCGCTGGCA 
CTGCAGCTGA TGGCCCAGGG ATTGTACAAC CACACCACCG TGCCGCTGAC CAAGGAGGAG 
GAGGAGCTCG AGATGCGCAT GAACGGGCAG ATCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725059.1
CDS	541..2199
Translation	MALEDRCSPQSAPSPITLQMQHLHHQQQQQQQQQQQMQHLHQLQQLQQLHQQQLAAGVFHHPAMAFDAAAAAAAAAAAAAAHAHAAALQQRLSGSGSPASCSTPASSTPLTIKEEESDSVIGDMSFHNQTHTTNEEEEAEEDDDIDVDVDDTSAGGRLPPPAHQQQSTAKPSLAFSISNILSDRFGDVQKPGKSMENQASIFRPFEASRSQTATPSAFTRVDLLEFSRQQQAAAAAATAAMMLERANFLNCFNPAAYPRIHEEIVQSRLRRSAANAVIPPPMSSKMSDANPEKSALGSLCKAVSQIGQPAAPTMTQPPLSSSASSLASPPPASNASTISSTSSVATSSSSSSSGCSSAASSLNSSPSSRLGASGSGVNASSPQPQPIPPPSAVSRDSGMESSDDTRSETGSTTTEGGKNEMWPAWVYCTRYSDRPSSGPRYRRPKQPKDKTNDEKRPRTAFSSEQLARLKREFNENRYLTERRRQQLSSELGLNEAQIKIWFQNKRAKIKKSTGSKNPLALQLMAQGLYNHTTVPLTKEEEELEMRMNGQIP

Target ORF information:

RefSeq Version	NM_165841.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster engrailed (en), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165841.2

ORF Insert Sequence:

ATGGCCCTGG AGGATCGCTG CAGTCCACAG TCAGCGCCCA GCCCCATTAC CCTACAAATG 
CAGCATCTTC ACCACCAGCA ACAGCAGCAG CAGCAACAGC AGCAGCAAAT GCAGCACCTC 
CACCAGCTGC AGCAACTGCA GCAGTTGCAC CAACAGCAAC TGGCCGCCGG TGTCTTCCAC 
CATCCGGCAA TGGCCTTCGA TGCCGCTGCA GCCGCCGCTG CTGCAGCTGC TGCTGCGGCC 
GCCCACGCTC ATGCTGCTGC ACTGCAGCAG CGCCTCAGTG GCAGTGGATC GCCCGCATCC 
TGCTCCACGC CCGCCTCGTC CACGCCGCTG ACCATCAAGG AGGAGGAAAG CGACTCCGTG 
ATCGGTGACA TGAGTTTCCA CAATCAGACG CACACCACCA ACGAGGAGGA GGAGGCGGAG 
GAGGATGACG ACATTGATGT GGATGTGGAT GATACGTCGG CGGGCGGACG CCTGCCACCA 
CCCGCCCACC AGCAGCAGTC GACGGCCAAG CCCTCGCTGG CCTTTTCCAT CTCCAACATC 
CTGAGCGATC GTTTCGGAGA TGTCCAGAAG CCGGGCAAGT CGATGGAGAA CCAGGCCAGC 
ATATTCCGCC CCTTCGAGGC GAGTCGTTCC CAGACTGCCA CGCCCTCCGC CTTTACAAGA 
GTGGATCTGC TGGAGTTTAG CCGGCAACAG CAGGCTGCCG CCGCAGCCGC TACTGCGGCC 
ATGATGCTGG AACGGGCCAA CTTCCTTAAC TGCTTCAATC CGGCTGCCTA TCCCAGGATA 
CACGAGGAAA TCGTGCAGAG TCGGCTGCGC AGGAGTGCAG CCAATGCCGT CATCCCGCCG 
CCCATGAGCT CCAAGATGAG CGATGCCAAT CCAGAGAAAT CTGCTCTGGG ATCCCTGTGC 
AAGGCGGTCT CGCAGATCGG ACAACCTGCT GCCCCTACGA TGACCCAACC TCCGCTGAGT 
AGCAGTGCCA GCAGCTTGGC CAGTCCGCCA CCCGCCTCCA ATGCCTCGAC CATTAGCAGC 
ACCTCTTCCG TGGCCACCAG CTCGAGCTCC TCCTCGTCGG GTTGCTCCTC GGCGGCCAGT 
TCCTTGAACT CCTCGCCCAG TAGCCGACTG GGAGCCAGTG GATCCGGAGT CAATGCCAGC 
AGTCCCCAGC CGCAGCCAAT CCCGCCGCCA TCCGCCGTTA GCCGAGATTC CGGAATGGAG 
TCCTCGGATG ACACGCGTTC CGAGACGGGA TCCACCACCA CAGAGGGCGG CAAGAACGAG 
ATGTGGCCCG CCTGGGTGTA CTGCACCCGC TACAGCGATC GTCCCAGCTC AGGACCCCGC 
TACCGCCGCC CCAAACAGCC AAAGGACAAG ACCAACGACG AGAAGCGTCC ACGCACCGCG 
TTCTCCAGCG AGCAGTTGGC CCGCCTTAAG CGGGAGTTCA ACGAGAATCG CTATCTGACC 
GAGCGGAGAC GCCAGCAGCT GAGCAGCGAG TTGGGCCTGA ACGAGGCGCA GATCAAGATC 
TGGTTCCAGA ACAAGCGGGC CAAGATCAAG AAGTCGACGG GCTCCAAAAA TCCGCTGGCA 
CTGCAGCTGA TGGCCCAGGG ATTGTACAAC CACACCACCG TGCCGCTGAC CAAGGAGGAG 
GAGGAGCTCG AGATGCGCAT GAACGGGCAG ATCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

en ( NM_078976.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523700.2 Drosophila melanogaster engrailed (en), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09618
Clone ID Related Accession (Same CDS sequence)	NM_165841.2 , NM_078976.4
Accession Version	NM_078976.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	engrailed, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078976.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCTGG AGGATCGCTG CAGTCCACAG TCAGCGCCCA GCCCCATTAC CCTACAAATG 
CAGCATCTTC ACCACCAGCA ACAGCAGCAG CAGCAACAGC AGCAGCAAAT GCAGCACCTC 
CACCAGCTGC AGCAACTGCA GCAGTTGCAC CAACAGCAAC TGGCCGCCGG TGTCTTCCAC 
CATCCGGCAA TGGCCTTCGA TGCCGCTGCA GCCGCCGCTG CTGCAGCTGC TGCTGCGGCC 
GCCCACGCTC ATGCTGCTGC ACTGCAGCAG CGCCTCAGTG GCAGTGGATC GCCCGCATCC 
TGCTCCACGC CCGCCTCGTC CACGCCGCTG ACCATCAAGG AGGAGGAAAG CGACTCCGTG 
ATCGGTGACA TGAGTTTCCA CAATCAGACG CACACCACCA ACGAGGAGGA GGAGGCGGAG 
GAGGATGACG ACATTGATGT GGATGTGGAT GATACGTCGG CGGGCGGACG CCTGCCACCA 
CCCGCCCACC AGCAGCAGTC GACGGCCAAG CCCTCGCTGG CCTTTTCCAT CTCCAACATC 
CTGAGCGATC GTTTCGGAGA TGTCCAGAAG CCGGGCAAGT CGATGGAGAA CCAGGCCAGC 
ATATTCCGCC CCTTCGAGGC GAGTCGTTCC CAGACTGCCA CGCCCTCCGC CTTTACAAGA 
GTGGATCTGC TGGAGTTTAG CCGGCAACAG CAGGCTGCCG CCGCAGCCGC TACTGCGGCC 
ATGATGCTGG AACGGGCCAA CTTCCTTAAC TGCTTCAATC CGGCTGCCTA TCCCAGGATA 
CACGAGGAAA TCGTGCAGAG TCGGCTGCGC AGGAGTGCAG CCAATGCCGT CATCCCGCCG 
CCCATGAGCT CCAAGATGAG CGATGCCAAT CCAGAGAAAT CTGCTCTGGG ATCCCTGTGC 
AAGGCGGTCT CGCAGATCGG ACAACCTGCT GCCCCTACGA TGACCCAACC TCCGCTGAGT 
AGCAGTGCCA GCAGCTTGGC CAGTCCGCCA CCCGCCTCCA ATGCCTCGAC CATTAGCAGC 
ACCTCTTCCG TGGCCACCAG CTCGAGCTCC TCCTCGTCGG GTTGCTCCTC GGCGGCCAGT 
TCCTTGAACT CCTCGCCCAG TAGCCGACTG GGAGCCAGTG GATCCGGAGT CAATGCCAGC 
AGTCCCCAGC CGCAGCCAAT CCCGCCGCCA TCCGCCGTTA GCCGAGATTC CGGAATGGAG 
TCCTCGGATG ACACGCGTTC CGAGACGGGA TCCACCACCA CAGAGGGCGG CAAGAACGAG 
ATGTGGCCCG CCTGGGTGTA CTGCACCCGC TACAGCGATC GTCCCAGCTC AGGACCCCGC 
TACCGCCGCC CCAAACAGCC AAAGGACAAG ACCAACGACG AGAAGCGTCC ACGCACCGCG 
TTCTCCAGCG AGCAGTTGGC CCGCCTTAAG CGGGAGTTCA ACGAGAATCG CTATCTGACC 
GAGCGGAGAC GCCAGCAGCT GAGCAGCGAG TTGGGCCTGA ACGAGGCGCA GATCAAGATC 
TGGTTCCAGA ACAAGCGGGC CAAGATCAAG AAGTCGACGG GCTCCAAAAA TCCGCTGGCA 
CTGCAGCTGA TGGCCCAGGG ATTGTACAAC CACACCACCG TGCCGCTGAC CAAGGAGGAG 
GAGGAGCTCG AGATGCGCAT GAACGGGCAG ATCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523700.2
CDS	214..1872
Translation	MALEDRCSPQSAPSPITLQMQHLHHQQQQQQQQQQQMQHLHQLQQLQQLHQQQLAAGVFHHPAMAFDAAAAAAAAAAAAAAHAHAAALQQRLSGSGSPASCSTPASSTPLTIKEEESDSVIGDMSFHNQTHTTNEEEEAEEDDDIDVDVDDTSAGGRLPPPAHQQQSTAKPSLAFSISNILSDRFGDVQKPGKSMENQASIFRPFEASRSQTATPSAFTRVDLLEFSRQQQAAAAAATAAMMLERANFLNCFNPAAYPRIHEEIVQSRLRRSAANAVIPPPMSSKMSDANPEKSALGSLCKAVSQIGQPAAPTMTQPPLSSSASSLASPPPASNASTISSTSSVATSSSSSSSGCSSAASSLNSSPSSRLGASGSGVNASSPQPQPIPPPSAVSRDSGMESSDDTRSETGSTTTEGGKNEMWPAWVYCTRYSDRPSSGPRYRRPKQPKDKTNDEKRPRTAFSSEQLARLKREFNENRYLTERRRQQLSSELGLNEAQIKIWFQNKRAKIKKSTGSKNPLALQLMAQGLYNHTTVPLTKEEEELEMRMNGQIP

Target ORF information:

RefSeq Version	NM_078976.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster engrailed (en), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078976.4

ORF Insert Sequence:

ATGGCCCTGG AGGATCGCTG CAGTCCACAG TCAGCGCCCA GCCCCATTAC CCTACAAATG 
CAGCATCTTC ACCACCAGCA ACAGCAGCAG CAGCAACAGC AGCAGCAAAT GCAGCACCTC 
CACCAGCTGC AGCAACTGCA GCAGTTGCAC CAACAGCAAC TGGCCGCCGG TGTCTTCCAC 
CATCCGGCAA TGGCCTTCGA TGCCGCTGCA GCCGCCGCTG CTGCAGCTGC TGCTGCGGCC 
GCCCACGCTC ATGCTGCTGC ACTGCAGCAG CGCCTCAGTG GCAGTGGATC GCCCGCATCC 
TGCTCCACGC CCGCCTCGTC CACGCCGCTG ACCATCAAGG AGGAGGAAAG CGACTCCGTG 
ATCGGTGACA TGAGTTTCCA CAATCAGACG CACACCACCA ACGAGGAGGA GGAGGCGGAG 
GAGGATGACG ACATTGATGT GGATGTGGAT GATACGTCGG CGGGCGGACG CCTGCCACCA 
CCCGCCCACC AGCAGCAGTC GACGGCCAAG CCCTCGCTGG CCTTTTCCAT CTCCAACATC 
CTGAGCGATC GTTTCGGAGA TGTCCAGAAG CCGGGCAAGT CGATGGAGAA CCAGGCCAGC 
ATATTCCGCC CCTTCGAGGC GAGTCGTTCC CAGACTGCCA CGCCCTCCGC CTTTACAAGA 
GTGGATCTGC TGGAGTTTAG CCGGCAACAG CAGGCTGCCG CCGCAGCCGC TACTGCGGCC 
ATGATGCTGG AACGGGCCAA CTTCCTTAAC TGCTTCAATC CGGCTGCCTA TCCCAGGATA 
CACGAGGAAA TCGTGCAGAG TCGGCTGCGC AGGAGTGCAG CCAATGCCGT CATCCCGCCG 
CCCATGAGCT CCAAGATGAG CGATGCCAAT CCAGAGAAAT CTGCTCTGGG ATCCCTGTGC 
AAGGCGGTCT CGCAGATCGG ACAACCTGCT GCCCCTACGA TGACCCAACC TCCGCTGAGT 
AGCAGTGCCA GCAGCTTGGC CAGTCCGCCA CCCGCCTCCA ATGCCTCGAC CATTAGCAGC 
ACCTCTTCCG TGGCCACCAG CTCGAGCTCC TCCTCGTCGG GTTGCTCCTC GGCGGCCAGT 
TCCTTGAACT CCTCGCCCAG TAGCCGACTG GGAGCCAGTG GATCCGGAGT CAATGCCAGC 
AGTCCCCAGC CGCAGCCAAT CCCGCCGCCA TCCGCCGTTA GCCGAGATTC CGGAATGGAG 
TCCTCGGATG ACACGCGTTC CGAGACGGGA TCCACCACCA CAGAGGGCGG CAAGAACGAG 
ATGTGGCCCG CCTGGGTGTA CTGCACCCGC TACAGCGATC GTCCCAGCTC AGGACCCCGC 
TACCGCCGCC CCAAACAGCC AAAGGACAAG ACCAACGACG AGAAGCGTCC ACGCACCGCG 
TTCTCCAGCG AGCAGTTGGC CCGCCTTAAG CGGGAGTTCA ACGAGAATCG CTATCTGACC 
GAGCGGAGAC GCCAGCAGCT GAGCAGCGAG TTGGGCCTGA ACGAGGCGCA GATCAAGATC 
TGGTTCCAGA ACAAGCGGGC CAAGATCAAG AAGTCGACGG GCTCCAAAAA TCCGCTGGCA 
CTGCAGCTGA TGGCCCAGGG ATTGTACAAC CACACCACCG TGCCGCTGAC CAAGGAGGAG 
GAGGAGCTCG AGATGCGCAT GAACGGGCAG ATCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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en cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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