sha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sha
Entrez Gene ID	36213
Full Name	shavenoid
Synonyms	CG13209,Dmel\CG13209,koj,lincRNA.286
General protein information	Preferred Names shavenoid Names CG13209 gene product from transcript CG13209-RB CG13209-PA CG13209-PB CG13209-PC kojak sha-PA sha-PB sha-PC shavenoid/kojak
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47F2-47F3\|2-62 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273960.1	NP_001260889.1	shavenoid, isoform C
NM_136828.3	NP_610672.1	shavenoid, isoform A
NM_001144173.3	NP_001137645.1	shavenoid, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

sha (also known as kojak) is necessary but not sufficient to activate the cytoskeleton to drive wing hair formation.
Title: The shavenoid gene of Drosophila encodes a novel actin cytoskeleton interacting protein that promotes wing hair morphogenesis.

The following sha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09590	NM_001144173.3 Latest version!	Drosophila melanogaster shavenoid (sha), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa09591	NM_136828.3 Latest version!	Drosophila melanogaster shavenoid (sha), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa12159	NM_001273960.1 Latest version!	Drosophila melanogaster shavenoid (sha), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sha ( NM_001144173.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137645.1 Drosophila melanogaster shavenoid (sha), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09590
Clone ID Related Accession (Same CDS sequence)	NM_001144173.3
Accession Version	NM_001144173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shavenoid, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144173.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG AGAAAATCCA CCAATCCATA 
GATGGACTCA ATCCGGCGAC CTCGTCTTTG GGCGGCGGTC AAATGGAGCT CCTGACCCGT 
CGTCTCAAGT TGCAGAAGGG TCGCGGTCAA GTGGCGAATA TAGATGCGAG CGGTGAGACT 
GGGTGCACCT GCCGATGTCT TCCCTATCAG CGGGCCTATC GCGAGGATCT GGGCATATGC 
GTGGACGACA TCCATGAGTG CTCCCTGTCG CCATTCGTGA GTGGTTCCTC CTCGGAAAAG 
ATACCCTTTG TCTTCCTGCC GCTCAAGGGT CAGATCATCT ATCCCAGCAG GGAGATCAGC 
TTTGCCAGCA TTCACACACC CGTTTGCGCG GTAACCGGAG CCCAGTACCT GGGTTCAAAT 
GGCTGGTCGG ATCTGCGGAA CCCCATAGAC ACCGACTACC CGTTTCGCAT GTTCCGCGAT 
GAGGGACGCA GCTTCCTCTT GTGGCTGGGT GAAGCTGATC TGCGCCAGAA GATGCAGGGT 
CGCCTGATTG TGGTGCATCT GGTGTGTCGC GACATGACGG TGGCCTTGAA TGCCAGCAAT 
CACGTCAAGG GTGAACCCCT CATGCCGCCC AGGAATGTTC ACTCACCATG TGTGGCTTTC 
AGAGTGAATG GCAGTCCCGT AAAATATGCC CACAATGTCC CCGAGGTCTT TTTCCAACCA 
GCCAACTCCA CCACTTTGGC CTCCACTTCG GATGGCATGA CCATGCGTGA GTACGTGGTC 
ATTGGGATCT GTAGCCTGCT CTTGGGTCTC ATCTATGTGG CCTCAGTGTT TCTATATCTT 
TACATGAAGA AACGAAAGCG GCACAGCTCT CGTCATTCCC TAGACAATCT GACAAACGAC 
ATCAATTATC CTAAGAATGA TCAGGTTACG TATGGAGCAC CCTTTAGCCG AGTGGGTAGC 
ATATACTCCT CCAATAGCAT GGGTCTGGGA AATGGAAATG AGTCCAATAC ACGTACAAGT 
ATAGGCAGTT TAAAGGAGGA TATGGGCATT GTGAAAAATA ATCCATTGCT GCAGCATTTT 
CCACAGTTGA GTGAACGAGA ACACAATTCC GGGTTTGCCA GCGATATTTC AAATTCGAAT 
TCTGAGTGTG AAATGGAGGA AAAGATCAAG ACATCCGTGC TGGTGCATCC ACAGCTCAGT 
AAGTCGCCGA AGCCTTCGGG TGGAACAGAT AACGCTGCAA TGGATGTGGA TGACTTCCTG 
CAGGACAACG AGTGTCTGCC CGCTGAAAAT GTGGCCGTAA TTGATGAGCT CATGAGCGAG 
GAGAAGCTGG AGAACTTGCG TGCCATGGTC AGTGGTAATG TGCGCAAGAA GCTCTACTTC 
AATCCCGCTT ATTTTGAGCC TCATCTACTA GCGGAACCGC CTCAAGCTGC CATTGAGTTC 
CTGCAGAAGA TTCGCGAGGT CATCGCCATT GCCAAGTACA AAATGGCCTC AAAGCGTTAC 
CAACCCTCTC TGAACATAAT TCCGGAGGAG CAGCATCCGG AGTCCAGTTC CAGTTCACCC 
ATGTTCAATA TTCCGCTGCA GCAGAGTCTG GCGGGGAAGG GAATGGGCGA TAAGCGGAAC 
AGCATCGAGC AGTGGCTGCA AAGTGTACCA AACTCGGATG CGACTCCGGC ACCGAAGACG 
CTCGACCGAC CTGCTCCACC GACTGCAAAG AAGCCATCAT CCGGATCTCC CAACAAGGGC 
TCATTGAGAA AGGAGATAAG TGCACCCAAG GAGCGACCAC CACCACCTCC AATGCAGAAA 
TCAAAAGCAG ATGAAGGTAA TGCAAAGTCA TCTTCTCCAG CGCCTTCGGG TCCACATTCC 
AGCCGAAGCA CCGGAAAGAG CAGTCCAGAA CAGTCACCCA AGCCGAAGAC AAATGTGGGT 
AGCACTTATG AGGGATTCTC TGCCTTTTCC GTGGCCGCCA ATGTCTATGG ATTTGCAGAG 
ACCGGCGGGG AAATCTACAA TAATCCCAAG TTCATGCTGG CAGACTCACC TGCGGCCTCA 
TTGCGCAGTG CCTCCAGTCG CTCCAAATCG ATGAAGAAGC GTAGCGAGGT TCTGGATCAG 
TTCGCTGCCG CCAACTCCAC ACCCACCTCG CTCACCTCCT TCGATCGGCA GCACTACAAC 
ATGCTGAGAA ACATGAAAGC GCCGGAGGTC ATCTACGATT CCTTGAAGCG CGAGTTTGCA 
GCGCATATTC CAACGCCTGA TTATGATAGC ACCATAGAGA AACGAATCAA AGACATCTAT 
AGCAGTACGC AGAGTAGCAT ACCATCGGTG CCCACTCCGG ATTACAGCTC TCTCAGCCGT 
AAATCTCTTA AGCAGTTCCA ACCCGATTCC CCGATTTATA GAAGGAAGTC CCCGCAGTAT 
CTGATTGTGG ACTACGAAAC GGATAGTCTT GAACGTCTGG AACCCAGCAA ACGCAAGAGT 
TCCCACTCGT CCAGTTCACC GTCATCCGAT GTGAGTTCCC AATTGAGTCC AAGCTTGAGT 
ACCGCTCTTC CGCTCGAGGA GGAGATGGAG ATCAGTCACA CTGTATACGA TCGTATGAAC 
GGATTTCGCA AGGAGGGCGA GATGAAAAAG CGTTTGGCTG CAAAGCACCA ACATCCACAT 
CCTGGGCAGC AACAAAGGCA GAAGGAGGCG GCAGCTCGAA TCAAATATGA CACGCCCTTT 
CGGGGAAGCA TGACCATCGA GGTGGAACAT GAACCCCCTT CCGATCTGGA GCGAACGGAT 
AGCGATCAAT TCGAACCGGA TACACTGGAT CGTAAGCCCA AGAAGCAGAG CTTCTGCGAC 
ATTTCAGCTT GGGATGGGCA TAAGCCAATC GACTCGGACT TTAGCCAGAT CAGTTCTCTG 
CCGGATTTGA GTCGGCAAAT CAGTCCTGCA CCCAATCAGG GCATTAAACC AAGGACCGCT 
TCCTTCATCC TTCGATCCAG CAACTCATTT AGGAACTTGA GAGAGATCTA TGAGTCGCCT 
TTAATCGCTC AGGACAACAT CAAGTACGAG AAGAAGGTTG CACAAACACC GGAAGAGGAG 
GGGCGTATTC TCACCCTGGA GGCCAAGCAC TCTCGTCGCC AGCGTCTGAT GGAGACGTCA 
CCAACCCACT CAGTGATGGA TATCACAAAA GTAAACAAGA CCACTATTAC GATTTCATCA 
CCGAAGCCAG CGGATAAAGC TCCTCCTACT CGACCAAAGG TCAATCAGCA TTACTGCCCA 
CCGTTGGTGC AAAGCAAACG ACCACTACCA CCTCCTCCCC CAAACTCATC GGAACATCAG 
GCTCCAGATG AAGATGCCTA CAGCCTGCAC AGTGAGATCA CAGGCACCTC GGAGTTCGAG 
AACTCCTGCA ACAACACCAT GTCCAGTGCT ATATCGGCAA CCACCGAGGA GAGAACTCAT 
GCAAAGGGTT CCAGATCCCC GAAAATCAAT GCTAAGATAC CGGAAGAGGA CTACGAAGGG 
ATGTACAACA AAAAGATCAA CAGCCTGCGC AACGACTACA GTCTGACGAA GTATCTCAAT 
CGACGGAAAA GTCAGCGGGA GGAGGTTTCA AAAGCTGGTC CGGATTCCCC AAAGGACGTT 
GCCCCCGAGC CGGAGTTCGG TGGCAGCAAT AAGAGCGTAA AGGAGGTGGA TGTGGCCCAG 
TTCGATGCGC TGTCCATAAG CTCACGCTCG AATCGGAGTA GCAGTCATCT TTCCGAGCGA 
ACAAAAGAGA TGTACAGGAA CGCCGGAGTT CCAGTGGGCA TTGCCTATCC CCAACGAGCG 
GCTAGTAGTT CCGGAAATGC TAGTGCCGGC GGAAACGTTC AAACTGTGTA TCGCTGTGAA 
ATCGAACAAG CCCAGCTCAC GGCTGGAATG CAGATTGCCA TTGGACTAAA GGATCGCGCC 
AAGAAAGCCA AGGACCTGAA GAATGCGTGG CGAAAGTTTG TCACTATGGC GGCCAACAAG 
TTTAGTCCCA GCCAAAGTCC AGCACCAACA TATGGTAGCA AAACTGCTGC CGGGTTCCTC 
GAGGATGACG GCATTGCCTC CATTATCGAA GATGCAGCTC AGTCCGCTGG AATGGGACAA 
CCGGGTAGCC AGACGTATTA CGAGCAACGC TTTGGTCAAC TGGACAGCGG ATACATGAGC 
ACCGATTCAA CGGAACTGTA CAAGCGAAAT GTCTACGATC GGTATAATTT CAAGATGCCG 
TGTGGACGCG GACAGATCAT TCGGGTGGAC ACCATCGAGG ACAACGAGGA GGAAAGCGGT 
CCGAATGACA ATCGGTTCGA GGACCTCGAG CACATGGTCT CTCCAAGTCA CAGTCGTCGA 
TCGCCGGAAA ACAACGCAGT CGTTGCTTGT GATCTCAGCG ATGCGGAATC TGATAAAACT 
CTGACCCGTT CCCATTCCCA AACGAATTCC CAGGAGCGAA ACGTGCGGGG CAGCACCACA 
ACAATGTCAT CCTACTCCTC GGATGAAAAT GGACGCGGAC GAAGTACCTG CTGTGGATCT 
TCGGAAACGG ACGAGGAGAC CAACGCGGAG GACATGTGGG AGTCGGGGGC AGAGAGCATA 
GAAACGCACT CTGTGCTCTA CAAGATGATT AGGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137645.1
CDS	726..5627
Translation	MRLQSLPLLLLLAVVTLPPATPTSAARPLTHPRARNHYPSEDLAEEPLMPASLLLEELQQTNGQLVNLTRQHDGDTFHTSGLSSACNADTCIGLSSGTASLVRSTGDGSGQGNEKIHQSIDGLNPATSSLGGGQMELLTRRLKLQKGRGQVANIDASGETGCTCRCLPYQRAYREDLGICVDDIHECSLSPFVSGSSSEKIPFVFLPLKGQIIYPSREISFASIHTPVCAVTGAQYLGSNGWSDLRNPIDTDYPFRMFRDEGRSFLLWLGEADLRQKMQGRLIVVHLVCRDMTVALNASNHVKGEPLMPPRNVHSPCVAFRVNGSPVKYAHNVPEVFFQPANSTTLASTSDGMTMREYVVIGICSLLLGLIYVASVFLYLYMKKRKRHSSRHSLDNLTNDINYPKNDQVTYGAPFSRVGSIYSSNSMGLGNGNESNTRTSIGSLKEDMGIVKNNPLLQHFPQLSEREHNSGFASDISNSNSECEMEEKIKTSVLVHPQLSKSPKPSGGTDNAAMDVDDFLQDNECLPAENVAVIDELMSEEKLENLRAMVSGNVRKKLYFNPAYFEPHLLAEPPQAAIEFLQKIREVIAIAKYKMASKRYQPSLNIIPEEQHPESSSSSPMFNIPLQQSLAGKGMGDKRNSIEQWLQSVPNSDATPAPKTLDRPAPPTAKKPSSGSPNKGSLRKEISAPKERPPPPPMQKSKADEGNAKSSSPAPSGPHSSRSTGKSSPEQSPKPKTNVGSTYEGFSAFSVAANVYGFAETGGEIYNNPKFMLADSPAASLRSASSRSKSMKKRSEVLDQFAAANSTPTSLTSFDRQHYNMLRNMKAPEVIYDSLKREFAAHIPTPDYDSTIEKRIKDIYSSTQSSIPSVPTPDYSSLSRKSLKQFQPDSPIYRRKSPQYLIVDYETDSLERLEPSKRKSSHSSSSPSSDVSSQLSPSLSTALPLEEEMEISHTVYDRMNGFRKEGEMKKRLAAKHQHPHPGQQQRQKEAAARIKYDTPFRGSMTIEVEHEPPSDLERTDSDQFEPDTLDRKPKKQSFCDISAWDGHKPIDSDFSQISSLPDLSRQISPAPNQGIKPRTASFILRSSNSFRNLREIYESPLIAQDNIKYEKKVAQTPEEEGRILTLEAKHSRRQRLMETSPTHSVMDITKVNKTTITISSPKPADKAPPTRPKVNQHYCPPLVQSKRPLPPPPPNSSEHQAPDEDAYSLHSEITGTSEFENSCNNTMSSAISATTEERTHAKGSRSPKINAKIPEEDYEGMYNKKINSLRNDYSLTKYLNRRKSQREEVSKAGPDSPKDVAPEPEFGGSNKSVKEVDVAQFDALSISSRSNRSSSHLSERTKEMYRNAGVPVGIAYPQRAASSSGNASAGGNVQTVYRCEIEQAQLTAGMQIAIGLKDRAKKAKDLKNAWRKFVTMAANKFSPSQSPAPTYGSKTAAGFLEDDGIASIIEDAAQSAGMGQPGSQTYYEQRFGQLDSGYMSTDSTELYKRNVYDRYNFKMPCGRGQIIRVDTIEDNEEESGPNDNRFEDLEHMVSPSHSRRSPENNAVVACDLSDAESDKTLTRSHSQTNSQERNVRGSTTTMSSYSSDENGRGRSTCCGSSETDEETNAEDMWESGAESIETHSVLYKMIRKS

Target ORF information:

RefSeq Version	NM_001144173.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shavenoid (sha), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144173.3

ORF Insert Sequence:

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG AGAAAATCCA CCAATCCATA 
GATGGACTCA ATCCGGCGAC CTCGTCTTTG GGCGGCGGTC AAATGGAGCT CCTGACCCGT 
CGTCTCAAGT TGCAGAAGGG TCGCGGTCAA GTGGCGAATA TAGATGCGAG CGGTGAGACT 
GGGTGCACCT GCCGATGTCT TCCCTATCAG CGGGCCTATC GCGAGGATCT GGGCATATGC 
GTGGACGACA TCCATGAGTG CTCCCTGTCG CCATTCGTGA GTGGTTCCTC CTCGGAAAAG 
ATACCCTTTG TCTTCCTGCC GCTCAAGGGT CAGATCATCT ATCCCAGCAG GGAGATCAGC 
TTTGCCAGCA TTCACACACC CGTTTGCGCG GTAACCGGAG CCCAGTACCT GGGTTCAAAT 
GGCTGGTCGG ATCTGCGGAA CCCCATAGAC ACCGACTACC CGTTTCGCAT GTTCCGCGAT 
GAGGGACGCA GCTTCCTCTT GTGGCTGGGT GAAGCTGATC TGCGCCAGAA GATGCAGGGT 
CGCCTGATTG TGGTGCATCT GGTGTGTCGC GACATGACGG TGGCCTTGAA TGCCAGCAAT 
CACGTCAAGG GTGAACCCCT CATGCCGCCC AGGAATGTTC ACTCACCATG TGTGGCTTTC 
AGAGTGAATG GCAGTCCCGT AAAATATGCC CACAATGTCC CCGAGGTCTT TTTCCAACCA 
GCCAACTCCA CCACTTTGGC CTCCACTTCG GATGGCATGA CCATGCGTGA GTACGTGGTC 
ATTGGGATCT GTAGCCTGCT CTTGGGTCTC ATCTATGTGG CCTCAGTGTT TCTATATCTT 
TACATGAAGA AACGAAAGCG GCACAGCTCT CGTCATTCCC TAGACAATCT GACAAACGAC 
ATCAATTATC CTAAGAATGA TCAGGTTACG TATGGAGCAC CCTTTAGCCG AGTGGGTAGC 
ATATACTCCT CCAATAGCAT GGGTCTGGGA AATGGAAATG AGTCCAATAC ACGTACAAGT 
ATAGGCAGTT TAAAGGAGGA TATGGGCATT GTGAAAAATA ATCCATTGCT GCAGCATTTT 
CCACAGTTGA GTGAACGAGA ACACAATTCC GGGTTTGCCA GCGATATTTC AAATTCGAAT 
TCTGAGTGTG AAATGGAGGA AAAGATCAAG ACATCCGTGC TGGTGCATCC ACAGCTCAGT 
AAGTCGCCGA AGCCTTCGGG TGGAACAGAT AACGCTGCAA TGGATGTGGA TGACTTCCTG 
CAGGACAACG AGTGTCTGCC CGCTGAAAAT GTGGCCGTAA TTGATGAGCT CATGAGCGAG 
GAGAAGCTGG AGAACTTGCG TGCCATGGTC AGTGGTAATG TGCGCAAGAA GCTCTACTTC 
AATCCCGCTT ATTTTGAGCC TCATCTACTA GCGGAACCGC CTCAAGCTGC CATTGAGTTC 
CTGCAGAAGA TTCGCGAGGT CATCGCCATT GCCAAGTACA AAATGGCCTC AAAGCGTTAC 
CAACCCTCTC TGAACATAAT TCCGGAGGAG CAGCATCCGG AGTCCAGTTC CAGTTCACCC 
ATGTTCAATA TTCCGCTGCA GCAGAGTCTG GCGGGGAAGG GAATGGGCGA TAAGCGGAAC 
AGCATCGAGC AGTGGCTGCA AAGTGTACCA AACTCGGATG CGACTCCGGC ACCGAAGACG 
CTCGACCGAC CTGCTCCACC GACTGCAAAG AAGCCATCAT CCGGATCTCC CAACAAGGGC 
TCATTGAGAA AGGAGATAAG TGCACCCAAG GAGCGACCAC CACCACCTCC AATGCAGAAA 
TCAAAAGCAG ATGAAGGTAA TGCAAAGTCA TCTTCTCCAG CGCCTTCGGG TCCACATTCC 
AGCCGAAGCA CCGGAAAGAG CAGTCCAGAA CAGTCACCCA AGCCGAAGAC AAATGTGGGT 
AGCACTTATG AGGGATTCTC TGCCTTTTCC GTGGCCGCCA ATGTCTATGG ATTTGCAGAG 
ACCGGCGGGG AAATCTACAA TAATCCCAAG TTCATGCTGG CAGACTCACC TGCGGCCTCA 
TTGCGCAGTG CCTCCAGTCG CTCCAAATCG ATGAAGAAGC GTAGCGAGGT TCTGGATCAG 
TTCGCTGCCG CCAACTCCAC ACCCACCTCG CTCACCTCCT TCGATCGGCA GCACTACAAC 
ATGCTGAGAA ACATGAAAGC GCCGGAGGTC ATCTACGATT CCTTGAAGCG CGAGTTTGCA 
GCGCATATTC CAACGCCTGA TTATGATAGC ACCATAGAGA AACGAATCAA AGACATCTAT 
AGCAGTACGC AGAGTAGCAT ACCATCGGTG CCCACTCCGG ATTACAGCTC TCTCAGCCGT 
AAATCTCTTA AGCAGTTCCA ACCCGATTCC CCGATTTATA GAAGGAAGTC CCCGCAGTAT 
CTGATTGTGG ACTACGAAAC GGATAGTCTT GAACGTCTGG AACCCAGCAA ACGCAAGAGT 
TCCCACTCGT CCAGTTCACC GTCATCCGAT GTGAGTTCCC AATTGAGTCC AAGCTTGAGT 
ACCGCTCTTC CGCTCGAGGA GGAGATGGAG ATCAGTCACA CTGTATACGA TCGTATGAAC 
GGATTTCGCA AGGAGGGCGA GATGAAAAAG CGTTTGGCTG CAAAGCACCA ACATCCACAT 
CCTGGGCAGC AACAAAGGCA GAAGGAGGCG GCAGCTCGAA TCAAATATGA CACGCCCTTT 
CGGGGAAGCA TGACCATCGA GGTGGAACAT GAACCCCCTT CCGATCTGGA GCGAACGGAT 
AGCGATCAAT TCGAACCGGA TACACTGGAT CGTAAGCCCA AGAAGCAGAG CTTCTGCGAC 
ATTTCAGCTT GGGATGGGCA TAAGCCAATC GACTCGGACT TTAGCCAGAT CAGTTCTCTG 
CCGGATTTGA GTCGGCAAAT CAGTCCTGCA CCCAATCAGG GCATTAAACC AAGGACCGCT 
TCCTTCATCC TTCGATCCAG CAACTCATTT AGGAACTTGA GAGAGATCTA TGAGTCGCCT 
TTAATCGCTC AGGACAACAT CAAGTACGAG AAGAAGGTTG CACAAACACC GGAAGAGGAG 
GGGCGTATTC TCACCCTGGA GGCCAAGCAC TCTCGTCGCC AGCGTCTGAT GGAGACGTCA 
CCAACCCACT CAGTGATGGA TATCACAAAA GTAAACAAGA CCACTATTAC GATTTCATCA 
CCGAAGCCAG CGGATAAAGC TCCTCCTACT CGACCAAAGG TCAATCAGCA TTACTGCCCA 
CCGTTGGTGC AAAGCAAACG ACCACTACCA CCTCCTCCCC CAAACTCATC GGAACATCAG 
GCTCCAGATG AAGATGCCTA CAGCCTGCAC AGTGAGATCA CAGGCACCTC GGAGTTCGAG 
AACTCCTGCA ACAACACCAT GTCCAGTGCT ATATCGGCAA CCACCGAGGA GAGAACTCAT 
GCAAAGGGTT CCAGATCCCC GAAAATCAAT GCTAAGATAC CGGAAGAGGA CTACGAAGGG 
ATGTACAACA AAAAGATCAA CAGCCTGCGC AACGACTACA GTCTGACGAA GTATCTCAAT 
CGACGGAAAA GTCAGCGGGA GGAGGTTTCA AAAGCTGGTC CGGATTCCCC AAAGGACGTT 
GCCCCCGAGC CGGAGTTCGG TGGCAGCAAT AAGAGCGTAA AGGAGGTGGA TGTGGCCCAG 
TTCGATGCGC TGTCCATAAG CTCACGCTCG AATCGGAGTA GCAGTCATCT TTCCGAGCGA 
ACAAAAGAGA TGTACAGGAA CGCCGGAGTT CCAGTGGGCA TTGCCTATCC CCAACGAGCG 
GCTAGTAGTT CCGGAAATGC TAGTGCCGGC GGAAACGTTC AAACTGTGTA TCGCTGTGAA 
ATCGAACAAG CCCAGCTCAC GGCTGGAATG CAGATTGCCA TTGGACTAAA GGATCGCGCC 
AAGAAAGCCA AGGACCTGAA GAATGCGTGG CGAAAGTTTG TCACTATGGC GGCCAACAAG 
TTTAGTCCCA GCCAAAGTCC AGCACCAACA TATGGTAGCA AAACTGCTGC CGGGTTCCTC 
GAGGATGACG GCATTGCCTC CATTATCGAA GATGCAGCTC AGTCCGCTGG AATGGGACAA 
CCGGGTAGCC AGACGTATTA CGAGCAACGC TTTGGTCAAC TGGACAGCGG ATACATGAGC 
ACCGATTCAA CGGAACTGTA CAAGCGAAAT GTCTACGATC GGTATAATTT CAAGATGCCG 
TGTGGACGCG GACAGATCAT TCGGGTGGAC ACCATCGAGG ACAACGAGGA GGAAAGCGGT 
CCGAATGACA ATCGGTTCGA GGACCTCGAG CACATGGTCT CTCCAAGTCA CAGTCGTCGA 
TCGCCGGAAA ACAACGCAGT CGTTGCTTGT GATCTCAGCG ATGCGGAATC TGATAAAACT 
CTGACCCGTT CCCATTCCCA AACGAATTCC CAGGAGCGAA ACGTGCGGGG CAGCACCACA 
ACAATGTCAT CCTACTCCTC GGATGAAAAT GGACGCGGAC GAAGTACCTG CTGTGGATCT 
TCGGAAACGG ACGAGGAGAC CAACGCGGAG GACATGTGGG AGTCGGGGGC AGAGAGCATA 
GAAACGCACT CTGTGCTCTA CAAGATGATT AGGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sha ( NM_136828.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610672.1 Drosophila melanogaster shavenoid (sha), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09591
Clone ID Related Accession (Same CDS sequence)	NM_136828.3
Accession Version	NM_136828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shavenoid, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136828.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG ATGGACTCAA TCCGGCGACC 
TCGTCTTTGG GCGGCGGTCA AATGGAGCTC CTGACCCGTC GTCTCAAGTT GCAGAAGGGT 
CGCGGTCAAG TGGCGAATAT AGATGCGAGC GGTGAGACTG GGTGCACCTG CCGATGTCTT 
CCCTATCAGC GGGCCTATCG CGAGGATCTG GGCATATGCG TGGACGACAT CCATGAGTGC 
TCCCTGTCGC CATTCGTGAG TGGTTCCTCC TCGGAAAAGA TACCCTTTGT CTTCCTGCCG 
CTCAAGGGTC AGATCATCTA TCCCAGCAGG GAGATCAGCT TTGCCAGCAT TCACACACCC 
GTTTGCGCGG TAACCGGAGC CCAGTACCTG GGTTCAAATG GCTGGTCGGA TCTGCGGAAC 
CCCATAGACA CCGACTACCC GTTTCGCATG TTCCGCGATG AGGGACGCAG CTTCCTCTTG 
TGGCTGGGTG AAGCTGATCT GCGCCAGAAG ATGCAGGGTC GCCTGATTGT GGTGCATCTG 
GTGTGTCGCG ACATGACGGT GGCCTTGAAT GCCAGCAATC ACGTCAAGGG TGAACCCCTC 
ATGCCGCCCA GGAATGTTCA CTCACCATGT GTGGCTTTCA GAGTGAATGG CAGTCCCGTA 
AAATATGCCC ACAATGTCCC CGAGGTCTTT TTCCAACCAG CCAACTCCAC CACTTTGGCC 
TCCACTTCGG ATGGCATGAC CATGCGTGAG TACGTGGTCA TTGGGATCTG TAGCCTGCTC 
TTGGGTCTCA TCTATGTGGC CTCAGTGTTT CTATATCTTT ACATGAAGAA ACGAAAGCGG 
CACAGCTCTC GTCATTCCCT AGACAATCTG ACAAACGACA TCAATTATCC TAAGAATGAT 
CAGGTTACGT ATGGAGCACC CTTTAGCCGA GTGGGTAGCA TATACTCCTC CAATAGCATG 
GGTCTGGGAA ATGGAAATGA GTCCAATACA CGTACAAGTA TAGGCAGTTT AAAGGAGGAT 
ATGGGCATTG TGAAAAATAA TCCATTGCTG CAGCATTTTC CACAGTTGAG TGAACGAGAA 
CACAATTCCG GGTTTGCCAG CGATATTTCA AATTCGAATT CTGAGTGTGA AATGGAGGAA 
AAGATCAAGA CATCCGTGCT GGTGCATCCA CAGCTCAGTA AGTCGCCGAA GCCTTCGGGT 
GGAACAGATA ACGCTGCAAT GGATGTGGAT GACTTCCTGC AGGACAACGA GTGTCTGCCC 
GCTGAAAATG TGGCCGTAAT TGATGAGCTC ATGAGCGAGG AGAAGCTGGA GAACTTGCGT 
GCCATGGTCA GTGGTAATGT GCGCAAGAAG CTCTACTTCA ATCCCGCTTA TTTTGAGCCT 
CATCTACTAG CGGAACCGCC TCAAGCTGCC ATTGAGTTCC TGCAGAAGAT TCGCGAGGTC 
ATCGCCATTG CCAAGTACAA AATGGCCTCA AAGCGTTACC AACCCTCTCT GAACATAATT 
CCGGAGGAGC AGCATCCGGA GTCCAGTTCC AGTTCACCCA TGTTCAATAT TCCGCTGCAG 
CAGAGTCTGG CGGGGAAGGG AATGGGCGAT AAGCGGAACA GCATCGAGCA GTGGCTGCAA 
AGTGTACCAA ACTCGGATGC GACTCCGGCA CCGAAGACGC TCGACCGACC TGCTCCACCG 
ACTGCAAAGA AGCCATCATC CGGATCTCCC AACAAGGGCT CATTGAGAAA GGAGATAAGT 
GCACCCAAGG AGCGACCACC ACCACCTCCA ATGCAGAAAT CAAAAGCAGA TGAAGGTAAT 
GCAAAGTCAT CTTCTCCAGC GCCTTCGGGT CCACATTCCA GCCGAAGCAC CGGAAAGAGC 
AGTCCAGAAC AGTCACCCAA GCCGAAGACA AATGTGGGTA GCACTTATGA GGGATTCTCT 
GCCTTTTCCG TGGCCGCCAA TGTCTATGGA TTTGCAGAGA CCGGCGGGGA AATCTACAAT 
AATCCCAAGT TCATGCTGGC AGACTCACCT GCGGCCTCAT TGCGCAGTGC CTCCAGTCGC 
TCCAAATCGA TGAAGAAGCG TAGCGAGGTT CTGGATCAGT TCGCTGCCGC CAACTCCACA 
CCCACCTCGC TCACCTCCTT CGATCGGCAG CACTACAACA TGCTGAGAAA CATGAAAGCG 
CCGGAGGTCA TCTACGATTC CTTGAAGCGC GAGTTTGCAG CGCATATTCC AACGCCTGAT 
TATGATAGCA CCATAGAGAA ACGAATCAAA GACATCTATA GCAGTACGCA GAGTAGCATA 
CCATCGGTGC CCACTCCGGA TTACAGCTCT CTCAGCCGTA AATCTCTTAA GCAGTTCCAA 
CCCGATTCCC CGATTTATAG AAGGAAGTCC CCGCAGTATC TGATTGTGGA CTACGAAACG 
GATAGTCTTG AACGTCTGGA ACCCAGCAAA CGCAAGAGTT CCCACTCGTC CAGTTCACCG 
TCATCCGATG TGAGTTCCCA ATTGAGTCCA AGCTTGAGTA CCGCTCTTCC GCTCGAGGAG 
GAGATGGAGA TCAGTCACAC TGTATACGAT CGTATGAACG GATTTCGCAA GGAGGGCGAG 
ATGAAAAAGC GTTTGGCTGC AAAGCACCAA CATCCACATC CTGGGCAGCA ACAAAGGCAG 
AAGGAGGCGG CAGCTCGAAT CAAATATGAC ACGCCCTTTC GGGGAAGCAT GACCATCGAG 
GTGGAACATG AACCCCCTTC CGATCTGGAG CGAACGGATA GCGATCAATT CGAACCGGAT 
ACACTGGATC GTAAGCCCAA GAAGCAGAGC TTCTGCGACA TTTCAGCTTG GGATGGGCAT 
AAGCCAATCG ACTCGGACTT TAGCCAGATC AGTTCTCTGC CGGATTTGAG TCGGCAAATC 
AGTCCTGCAC CCAATCAGGG CATTAAACCA AGGACCGCTT CCTTCATCCT TCGATCCAGC 
AACTCATTTA GGAACTTGAG AGAGATCTAT GAGTCGCCTT TAATCGCTCA GGACAACATC 
AAGTACGAGA AGAAGGTTGC ACAAACACCG GAAGAGGAGG GGCGTATTCT CACCCTGGAG 
GCCAAGCACT CTCGTCGCCA GCGTCTGATG GAGACGTCAC CAACCCACTC AGTGATGGAT 
ATCACAAAAG TAAACAAGAC CACTATTACG ATTTCATCAC CGAAGCCAGC GGATAAAGCT 
CCTCCTACTC GACCAAAGGT CAATCAGCAT TACTGCCCAC CGTTGGTGCA AAGCAAACGA 
CCACTACCAC CTCCTCCCCC AAACTCATCG GAACATCAGG CTCCAGATGA AGATGCCTAC 
AGCCTGCACA GTGAGATCAC AGGCACCTCG GAGTTCGAGA ACTCCTGCAA CAACACCATG 
TCCAGTGCTA TATCGGCAAC CACCGAGGAG AGAACTCATG CAAAGGGTTC CAGATCCCCG 
AAAATCAATG CTAAGATACC GGAAGAGGAC TACGAAGGGA TGTACAACAA AAAGATCAAC 
AGCCTGCGCA ACGACTACAG TCTGACGAAG TATCTCAATC GACGGAAAAG TCAGCGGGAG 
GAGGTTTCAA AAGCTGGTCC GGATTCCCCA AAGGACGTTG CCCCCGAGCC GGAGTTCGGT 
GGCAGCAATA AGAGCGTAAA GGAGGTGGAT GTGGCCCAGT TCGATGCGCT GTCCATAAGC 
TCACGCTCGA ATCGGAGTAG CAGTCATCTT TCCGAGCGAA CAAAAGAGAT GTACAGGAAC 
GCCGGAGTTC CAGTGGGCAT TGCCTATCCC CAACGAGCGG CTAGTAGTTC CGGAAATGCT 
AGTGCCGGCG GAAACGTTCA AACTGTGTAT CGCTGTGAAA TCGAACAAGC CCAGCTCACG 
GCTGGAATGC AGATTGCCAT TGGACTAAAG GATCGCGCCA AGAAAGCCAA GGACCTGAAG 
AATGCGTGGC GAAAGTTTGT CACTATGGCG GCCAACAAGT TTAGTCCCAG CCAAAGTCCA 
GCACCAACAT ATGGTAGCAA AACTGCTGCC GGGTTCCTCG AGGATGACGG CATTGCCTCC 
ATTATCGAAG ATGCAGCTCA GTCCGCTGGA ATGGGACAAC CGGGTAGCCA GACGTATTAC 
GAGCAACGCT TTGGTCAACT GGACAGCGGA TACATGAGCA CCGATTCAAC GGAACTGTAC 
AAGCGAAATG TCTACGATCG GTATAATTTC AAGATGCCGT GTGGACGCGG ACAGATCATT 
CGGGTGGACA CCATCGAGGA CAACGAGGAG GAAAGCGGTC CGAATGACAA TCGGTTCGAG 
GACCTCGAGC ACATGGTCTC TCCAAGTCAC AGTCGTCGAT CGCCGGAAAA CAACGCAGTC 
GTTGCTTGTG ATCTCAGCGA TGCGGAATCT GATAAAACTC TGACCCGTTC CCATTCCCAA 
ACGAATTCCC AGGAGCGAAA CGTGCGGGGC AGCACCACAA CAATGTCATC CTACTCCTCG 
GATGAAAATG GACGCGGACG AAGTACCTGC TGTGGATCTT CGGAAACGGA CGAGGAGACC 
AACGCGGAGG ACATGTGGGA GTCGGGGGCA GAGAGCATAG AAACGCACTC TGTGCTCTAC 
AAGATGATTA GGAAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610672.1
CDS	529..5409
Translation	MRLQSLPLLLLLAVVTLPPATPTSAARPLTHPRARNHYPSEDLAEEPLMPASLLLEELQQTNGQLVNLTRQHDGDTFHTSGLSSACNADTCIGLSSGTASLVRSTGDGSGQGNDGLNPATSSLGGGQMELLTRRLKLQKGRGQVANIDASGETGCTCRCLPYQRAYREDLGICVDDIHECSLSPFVSGSSSEKIPFVFLPLKGQIIYPSREISFASIHTPVCAVTGAQYLGSNGWSDLRNPIDTDYPFRMFRDEGRSFLLWLGEADLRQKMQGRLIVVHLVCRDMTVALNASNHVKGEPLMPPRNVHSPCVAFRVNGSPVKYAHNVPEVFFQPANSTTLASTSDGMTMREYVVIGICSLLLGLIYVASVFLYLYMKKRKRHSSRHSLDNLTNDINYPKNDQVTYGAPFSRVGSIYSSNSMGLGNGNESNTRTSIGSLKEDMGIVKNNPLLQHFPQLSEREHNSGFASDISNSNSECEMEEKIKTSVLVHPQLSKSPKPSGGTDNAAMDVDDFLQDNECLPAENVAVIDELMSEEKLENLRAMVSGNVRKKLYFNPAYFEPHLLAEPPQAAIEFLQKIREVIAIAKYKMASKRYQPSLNIIPEEQHPESSSSSPMFNIPLQQSLAGKGMGDKRNSIEQWLQSVPNSDATPAPKTLDRPAPPTAKKPSSGSPNKGSLRKEISAPKERPPPPPMQKSKADEGNAKSSSPAPSGPHSSRSTGKSSPEQSPKPKTNVGSTYEGFSAFSVAANVYGFAETGGEIYNNPKFMLADSPAASLRSASSRSKSMKKRSEVLDQFAAANSTPTSLTSFDRQHYNMLRNMKAPEVIYDSLKREFAAHIPTPDYDSTIEKRIKDIYSSTQSSIPSVPTPDYSSLSRKSLKQFQPDSPIYRRKSPQYLIVDYETDSLERLEPSKRKSSHSSSSPSSDVSSQLSPSLSTALPLEEEMEISHTVYDRMNGFRKEGEMKKRLAAKHQHPHPGQQQRQKEAAARIKYDTPFRGSMTIEVEHEPPSDLERTDSDQFEPDTLDRKPKKQSFCDISAWDGHKPIDSDFSQISSLPDLSRQISPAPNQGIKPRTASFILRSSNSFRNLREIYESPLIAQDNIKYEKKVAQTPEEEGRILTLEAKHSRRQRLMETSPTHSVMDITKVNKTTITISSPKPADKAPPTRPKVNQHYCPPLVQSKRPLPPPPPNSSEHQAPDEDAYSLHSEITGTSEFENSCNNTMSSAISATTEERTHAKGSRSPKINAKIPEEDYEGMYNKKINSLRNDYSLTKYLNRRKSQREEVSKAGPDSPKDVAPEPEFGGSNKSVKEVDVAQFDALSISSRSNRSSSHLSERTKEMYRNAGVPVGIAYPQRAASSSGNASAGGNVQTVYRCEIEQAQLTAGMQIAIGLKDRAKKAKDLKNAWRKFVTMAANKFSPSQSPAPTYGSKTAAGFLEDDGIASIIEDAAQSAGMGQPGSQTYYEQRFGQLDSGYMSTDSTELYKRNVYDRYNFKMPCGRGQIIRVDTIEDNEEESGPNDNRFEDLEHMVSPSHSRRSPENNAVVACDLSDAESDKTLTRSHSQTNSQERNVRGSTTTMSSYSSDENGRGRSTCCGSSETDEETNAEDMWESGAESIETHSVLYKMIRKS

Target ORF information:

RefSeq Version	NM_136828.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shavenoid (sha), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136828.3

ORF Insert Sequence:

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG ATGGACTCAA TCCGGCGACC 
TCGTCTTTGG GCGGCGGTCA AATGGAGCTC CTGACCCGTC GTCTCAAGTT GCAGAAGGGT 
CGCGGTCAAG TGGCGAATAT AGATGCGAGC GGTGAGACTG GGTGCACCTG CCGATGTCTT 
CCCTATCAGC GGGCCTATCG CGAGGATCTG GGCATATGCG TGGACGACAT CCATGAGTGC 
TCCCTGTCGC CATTCGTGAG TGGTTCCTCC TCGGAAAAGA TACCCTTTGT CTTCCTGCCG 
CTCAAGGGTC AGATCATCTA TCCCAGCAGG GAGATCAGCT TTGCCAGCAT TCACACACCC 
GTTTGCGCGG TAACCGGAGC CCAGTACCTG GGTTCAAATG GCTGGTCGGA TCTGCGGAAC 
CCCATAGACA CCGACTACCC GTTTCGCATG TTCCGCGATG AGGGACGCAG CTTCCTCTTG 
TGGCTGGGTG AAGCTGATCT GCGCCAGAAG ATGCAGGGTC GCCTGATTGT GGTGCATCTG 
GTGTGTCGCG ACATGACGGT GGCCTTGAAT GCCAGCAATC ACGTCAAGGG TGAACCCCTC 
ATGCCGCCCA GGAATGTTCA CTCACCATGT GTGGCTTTCA GAGTGAATGG CAGTCCCGTA 
AAATATGCCC ACAATGTCCC CGAGGTCTTT TTCCAACCAG CCAACTCCAC CACTTTGGCC 
TCCACTTCGG ATGGCATGAC CATGCGTGAG TACGTGGTCA TTGGGATCTG TAGCCTGCTC 
TTGGGTCTCA TCTATGTGGC CTCAGTGTTT CTATATCTTT ACATGAAGAA ACGAAAGCGG 
CACAGCTCTC GTCATTCCCT AGACAATCTG ACAAACGACA TCAATTATCC TAAGAATGAT 
CAGGTTACGT ATGGAGCACC CTTTAGCCGA GTGGGTAGCA TATACTCCTC CAATAGCATG 
GGTCTGGGAA ATGGAAATGA GTCCAATACA CGTACAAGTA TAGGCAGTTT AAAGGAGGAT 
ATGGGCATTG TGAAAAATAA TCCATTGCTG CAGCATTTTC CACAGTTGAG TGAACGAGAA 
CACAATTCCG GGTTTGCCAG CGATATTTCA AATTCGAATT CTGAGTGTGA AATGGAGGAA 
AAGATCAAGA CATCCGTGCT GGTGCATCCA CAGCTCAGTA AGTCGCCGAA GCCTTCGGGT 
GGAACAGATA ACGCTGCAAT GGATGTGGAT GACTTCCTGC AGGACAACGA GTGTCTGCCC 
GCTGAAAATG TGGCCGTAAT TGATGAGCTC ATGAGCGAGG AGAAGCTGGA GAACTTGCGT 
GCCATGGTCA GTGGTAATGT GCGCAAGAAG CTCTACTTCA ATCCCGCTTA TTTTGAGCCT 
CATCTACTAG CGGAACCGCC TCAAGCTGCC ATTGAGTTCC TGCAGAAGAT TCGCGAGGTC 
ATCGCCATTG CCAAGTACAA AATGGCCTCA AAGCGTTACC AACCCTCTCT GAACATAATT 
CCGGAGGAGC AGCATCCGGA GTCCAGTTCC AGTTCACCCA TGTTCAATAT TCCGCTGCAG 
CAGAGTCTGG CGGGGAAGGG AATGGGCGAT AAGCGGAACA GCATCGAGCA GTGGCTGCAA 
AGTGTACCAA ACTCGGATGC GACTCCGGCA CCGAAGACGC TCGACCGACC TGCTCCACCG 
ACTGCAAAGA AGCCATCATC CGGATCTCCC AACAAGGGCT CATTGAGAAA GGAGATAAGT 
GCACCCAAGG AGCGACCACC ACCACCTCCA ATGCAGAAAT CAAAAGCAGA TGAAGGTAAT 
GCAAAGTCAT CTTCTCCAGC GCCTTCGGGT CCACATTCCA GCCGAAGCAC CGGAAAGAGC 
AGTCCAGAAC AGTCACCCAA GCCGAAGACA AATGTGGGTA GCACTTATGA GGGATTCTCT 
GCCTTTTCCG TGGCCGCCAA TGTCTATGGA TTTGCAGAGA CCGGCGGGGA AATCTACAAT 
AATCCCAAGT TCATGCTGGC AGACTCACCT GCGGCCTCAT TGCGCAGTGC CTCCAGTCGC 
TCCAAATCGA TGAAGAAGCG TAGCGAGGTT CTGGATCAGT TCGCTGCCGC CAACTCCACA 
CCCACCTCGC TCACCTCCTT CGATCGGCAG CACTACAACA TGCTGAGAAA CATGAAAGCG 
CCGGAGGTCA TCTACGATTC CTTGAAGCGC GAGTTTGCAG CGCATATTCC AACGCCTGAT 
TATGATAGCA CCATAGAGAA ACGAATCAAA GACATCTATA GCAGTACGCA GAGTAGCATA 
CCATCGGTGC CCACTCCGGA TTACAGCTCT CTCAGCCGTA AATCTCTTAA GCAGTTCCAA 
CCCGATTCCC CGATTTATAG AAGGAAGTCC CCGCAGTATC TGATTGTGGA CTACGAAACG 
GATAGTCTTG AACGTCTGGA ACCCAGCAAA CGCAAGAGTT CCCACTCGTC CAGTTCACCG 
TCATCCGATG TGAGTTCCCA ATTGAGTCCA AGCTTGAGTA CCGCTCTTCC GCTCGAGGAG 
GAGATGGAGA TCAGTCACAC TGTATACGAT CGTATGAACG GATTTCGCAA GGAGGGCGAG 
ATGAAAAAGC GTTTGGCTGC AAAGCACCAA CATCCACATC CTGGGCAGCA ACAAAGGCAG 
AAGGAGGCGG CAGCTCGAAT CAAATATGAC ACGCCCTTTC GGGGAAGCAT GACCATCGAG 
GTGGAACATG AACCCCCTTC CGATCTGGAG CGAACGGATA GCGATCAATT CGAACCGGAT 
ACACTGGATC GTAAGCCCAA GAAGCAGAGC TTCTGCGACA TTTCAGCTTG GGATGGGCAT 
AAGCCAATCG ACTCGGACTT TAGCCAGATC AGTTCTCTGC CGGATTTGAG TCGGCAAATC 
AGTCCTGCAC CCAATCAGGG CATTAAACCA AGGACCGCTT CCTTCATCCT TCGATCCAGC 
AACTCATTTA GGAACTTGAG AGAGATCTAT GAGTCGCCTT TAATCGCTCA GGACAACATC 
AAGTACGAGA AGAAGGTTGC ACAAACACCG GAAGAGGAGG GGCGTATTCT CACCCTGGAG 
GCCAAGCACT CTCGTCGCCA GCGTCTGATG GAGACGTCAC CAACCCACTC AGTGATGGAT 
ATCACAAAAG TAAACAAGAC CACTATTACG ATTTCATCAC CGAAGCCAGC GGATAAAGCT 
CCTCCTACTC GACCAAAGGT CAATCAGCAT TACTGCCCAC CGTTGGTGCA AAGCAAACGA 
CCACTACCAC CTCCTCCCCC AAACTCATCG GAACATCAGG CTCCAGATGA AGATGCCTAC 
AGCCTGCACA GTGAGATCAC AGGCACCTCG GAGTTCGAGA ACTCCTGCAA CAACACCATG 
TCCAGTGCTA TATCGGCAAC CACCGAGGAG AGAACTCATG CAAAGGGTTC CAGATCCCCG 
AAAATCAATG CTAAGATACC GGAAGAGGAC TACGAAGGGA TGTACAACAA AAAGATCAAC 
AGCCTGCGCA ACGACTACAG TCTGACGAAG TATCTCAATC GACGGAAAAG TCAGCGGGAG 
GAGGTTTCAA AAGCTGGTCC GGATTCCCCA AAGGACGTTG CCCCCGAGCC GGAGTTCGGT 
GGCAGCAATA AGAGCGTAAA GGAGGTGGAT GTGGCCCAGT TCGATGCGCT GTCCATAAGC 
TCACGCTCGA ATCGGAGTAG CAGTCATCTT TCCGAGCGAA CAAAAGAGAT GTACAGGAAC 
GCCGGAGTTC CAGTGGGCAT TGCCTATCCC CAACGAGCGG CTAGTAGTTC CGGAAATGCT 
AGTGCCGGCG GAAACGTTCA AACTGTGTAT CGCTGTGAAA TCGAACAAGC CCAGCTCACG 
GCTGGAATGC AGATTGCCAT TGGACTAAAG GATCGCGCCA AGAAAGCCAA GGACCTGAAG 
AATGCGTGGC GAAAGTTTGT CACTATGGCG GCCAACAAGT TTAGTCCCAG CCAAAGTCCA 
GCACCAACAT ATGGTAGCAA AACTGCTGCC GGGTTCCTCG AGGATGACGG CATTGCCTCC 
ATTATCGAAG ATGCAGCTCA GTCCGCTGGA ATGGGACAAC CGGGTAGCCA GACGTATTAC 
GAGCAACGCT TTGGTCAACT GGACAGCGGA TACATGAGCA CCGATTCAAC GGAACTGTAC 
AAGCGAAATG TCTACGATCG GTATAATTTC AAGATGCCGT GTGGACGCGG ACAGATCATT 
CGGGTGGACA CCATCGAGGA CAACGAGGAG GAAAGCGGTC CGAATGACAA TCGGTTCGAG 
GACCTCGAGC ACATGGTCTC TCCAAGTCAC AGTCGTCGAT CGCCGGAAAA CAACGCAGTC 
GTTGCTTGTG ATCTCAGCGA TGCGGAATCT GATAAAACTC TGACCCGTTC CCATTCCCAA 
ACGAATTCCC AGGAGCGAAA CGTGCGGGGC AGCACCACAA CAATGTCATC CTACTCCTCG 
GATGAAAATG GACGCGGACG AAGTACCTGC TGTGGATCTT CGGAAACGGA CGAGGAGACC 
AACGCGGAGG ACATGTGGGA GTCGGGGGCA GAGAGCATAG AAACGCACTC TGTGCTCTAC 
AAGATGATTA GGAAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sha ( NM_001273960.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260889.1 Drosophila melanogaster shavenoid (sha), transcript variant C, mRNA.

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CloneID	OFa12159
Clone ID Related Accession (Same CDS sequence)	NM_001273960.1
Accession Version	NM_001273960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shavenoid, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG ATGGACTCAA TCCGGCGACC 
TCGTCTTTGG GCGGCGGTCA AATGGAGCTC CTGACCCGTC GTCTCAAGTT GCAGAAGGGT 
CGCGGTCAAG TGGCGAATAT AGATGCGAGC GGTGAGACTG GGTGCACCTG CCGATGTCTT 
CCCTATCAGC GGGCCTATCG CGAGGATCTG GGCATATGCG TGGACGACAT CCATGAGTGC 
TCCCTGTCGC CATTCGTGAG TGGTTCCTCC TCGGAAAAGA TACCCTTTGT CTTCCTGCCG 
CTCAAGGGTC AGATCATCTA TCCCAGCAGG GAGATCAGCT TTGCCAGCAT TCACACACCC 
GTTTGCGCGG TAACCGGAGC CCAGTACCTG GGTTCAAATG GCTGGTCGGA TCTGCGGAAC 
CCCATAGACA CCGACTACCC GTTTCGCATG TTCCGCGATG AGGGACGCAG CTTCCTCTTG 
TGGCTGGGTG AAGCTGATCT GCGCCAGAAG ATGCAGGGTC GCCTGATTGT GGTGCATCTG 
GTGTGTCGCG ACATGACGGT GGCCTTGAAT GCCAGCAATC ACGTCAAGGG TGAACCCCTC 
ATGCCGCCCA GGAATGTTCA CTCACCATGT GTGGCTTTCA GAGTGAATGG CAGTCCCGTA 
AAATATGCCC ACAATGTCCC CGAGGTCTTT TTCCAACCAG CCAACTCCAC CACTTTGGCC 
TCCACTTCGG ATGGCATGAC CATGCGTGAG TACGTGGTCA TTGGGATCTG TAGCCTGCTC 
TTGGGTCTCA TCTATGTGGC CTCAGTGTTT CTATATCTTT ACATGAAGAA ACGAAAGCGG 
CACAGCTCTC GTCATTCCCT AGACAATCTG ACAAACGACA TCAATTATCC TAAGAATGAT 
CAGGTTACGT ATGGAGCACC CTTTAGCCGA GTGGGTAGCA TATACTCCTC CAATAGCATG 
GGTCTGGGAA ATGGAAATGA GTCCAATACA CGTACAAGTA TAGGCAGTTT AAAGGAGGAT 
ATGGGCATTG TGAAAAATAA TCCATTGCTG CAGCATTTTC CACAGTTGAG TGAACGAGAA 
CACAATTCCG GGTTTGCCAG CGATATTTCA AATTCGAATT CTGAGTGTGA AATGGAGGAA 
AAGATCAAGC TCAGTAAGTC GCCGAAGCCT TCGGGTGGAA CAGATAACGC TGCAATGGAT 
GTGGATGACT TCCTGCAGGA CAACGAGTGT CTGCCCGCTG AAAATGTGGC CGTAATTGAT 
GAGCTCATGA GCGAGGAGAA GCTGGAGAAC TTGCGTGCCA TGGTCAGTGG TAATGTGCGC 
AAGAAGCTCT ACTTCAATCC CGCTTATTTT GAGCCTCATC TACTAGCGGA ACCGCCTCAA 
GCTGCCATTG AGTTCCTGCA GAAGATTCGC GAGGTCATCG CCATTGCCAA GTACAAAATG 
GCCTCAAAGC GTTACCAACC CTCTCTGAAC ATAATTCCGG AGGAGCAGCA TCCGGAGTCC 
AGTTCCAGTT CACCCATGTT CAATATTCCG CTGCAGCAGA GTCTGGCGGG GAAGGGAATG 
GGCGATAAGC GGAACAGCAT CGAGCAGTGG CTGCAAAGTG TACCAAACTC GGATGCGACT 
CCGGCACCGA AGACGCTCGA CCGACCTGCT CCACCGACTG CAAAGAAGCC ATCATCCGGA 
TCTCCCAACA AGGGCTCATT GAGAAAGGAG ATAAGTGCAC CCAAGGAGCG ACCACCACCA 
CCTCCAATGC AGAAATCAAA AGCAGATGAA GGTAATGCAA AGTCATCTTC TCCAGCGCCT 
TCGGGTCCAC ATTCCAGCCG AAGCACCGGA AAGAGCAGTC CAGAACAGTC ACCCAAGCCG 
AAGACAAATG TGGGTAGCAC TTATGAGGGA TTCTCTGCCT TTTCCGTGGC CGCCAATGTC 
TATGGATTTG CAGAGACCGG CGGGGAAATC TACAATAATC CCAAGTTCAT GCTGGCAGAC 
TCACCTGCGG CCTCATTGCG CAGTGCCTCC AGTCGCTCCA AATCGATGAA GAAGCGTAGC 
GAGGTTCTGG ATCAGTTCGC TGCCGCCAAC TCCACACCCA CCTCGCTCAC CTCCTTCGAT 
CGGCAGCACT ACAACATGCT GAGAAACATG AAAGCGCCGG AGGTCATCTA CGATTCCTTG 
AAGCGCGAGT TTGCAGCGCA TATTCCAACG CCTGATTATG ATAGCACCAT AGAGAAACGA 
ATCAAAGACA TCTATAGCAG TACGCAGAGT AGCATACCAT CGGTGCCCAC TCCGGATTAC 
AGCTCTCTCA GCCGTAAATC TCTTAAGCAG TTCCAACCCG ATTCCCCGAT TTATAGAAGG 
AAGTCCCCGC AGTATCTGAT TGTGGACTAC GAAACGGATA GTCTTGAACG TCTGGAACCC 
AGCAAACGCA AGAGTTCCCA CTCGTCCAGT TCACCGTCAT CCGATGTGAG TTCCCAATTG 
AGTCCAAGCT TGAGTACCGC TCTTCCGCTC GAGGAGGAGA TGGAGATCAG TCACACTGTA 
TACGATCGTA TGAACGGATT TCGCAAGGAG GGCGAGATGA AAAAGCGTTT GGCTGCAAAG 
CACCAACATC CACATCCTGG GCAGCAACAA AGGCAGAAGG AGGCGGCAGC TCGAATCAAA 
TATGACACGC CCTTTCGGGG AAGCATGACC ATCGAGGTGG AACATGAACC CCCTTCCGAT 
CTGGAGCGAA CGGATAGCGA TCAATTCGAA CCGGATACAC TGGATCGTAA GCCCAAGAAG 
CAGAGCTTCT GCGACATTTC AGCTTGGGAT GGGCATAAGC CAATCGACTC GGACTTTAGC 
CAGATCAGTT CTCTGCCGGA TTTGAGTCGG CAAATCAGTC CTGCACCCAA TCAGGGCATT 
AAACCAAGGA CCGCTTCCTT CATCCTTCGA TCCAGCAACT CATTTAGGAA CTTGAGAGAG 
ATCTATGAGT CGCCTTTAAT CGCTCAGGAC AACATCAAGT ACGAGAAGAA GGTTGCACAA 
ACACCGGAAG AGGAGGGGCG TATTCTCACC CTGGAGGCCA AGCACTCTCG TCGCCAGCGT 
CTGATGGAGA CGTCACCAAC CCACTCAGTG ATGGATATCA CAAAAGTAAA CAAGACCACT 
ATTACGATTT CATCACCGAA GCCAGCGGAT AAAGCTCCTC CTACTCGACC AAAGGTCAAT 
CAGCATTACT GCCCACCGTT GGTGCAAAGC AAACGACCAC TACCACCTCC TCCCCCAAAC 
TCATCGGAAC ATCAGGCTCC AGATGAAGAT GCCTACAGCC TGCACAGTGA GATCACAGGC 
ACCTCGGAGT TCGAGAACTC CTGCAACAAC ACCATGTCCA GTGCTATATC GGCAACCACC 
GAGGAGAGAA CTCATGCAAA GGGTTCCAGA TCCCCGAAAA TCAATGCTAA GATACCGGAA 
GAGGACTACG AAGGGATGTA CAACAAAAAG ATCAACAGCC TGCGCAACGA CTACAGTCTG 
ACGAAGTATC TCAATCGACG GAAAAGTCAG CGGGAGGAGG TTTCAAAAGC TGGTCCGGAT 
TCCCCAAAGG ACGTTGCCCC CGAGCCGGAG TTCGGTGGCA GCAATAAGAG CGTAAAGGAG 
GTGGATGTGG CCCAGTTCGA TGCGCTGTCC ATAAGCTCAC GCTCGAATCG GAGTAGCAGT 
CATCTTTCCG AGCGAACAAA AGAGATGTAC AGGAACGCCG GAGTTCCAGT GGGCATTGCC 
TATCCCCAAC GAGCGGCTAG TAGTTCCGGA AATGCTAGTG CCGGCGGAAA CGTTCAAACT 
GTGTATCGCT GTGAAATCGA ACAAGCCCAG CTCACGGCTG GAATGCAGAT TGCCATTGGA 
CTAAAGGATC GCGCCAAGAA AGCCAAGGAC CTGAAGAATG CGTGGCGAAA GTTTGTCACT 
ATGGCGGCCA ACAAGTTTAG TCCCAGCCAA AGTCCAGCAC CAACATATGG TAGCAAAACT 
GCTGCCGGGT TCCTCGAGGA TGACGGCATT GCCTCCATTA TCGAAGATGC AGCTCAGTCC 
GCTGGAATGG GACAACCGGG TAGCCAGACG TATTACGAGC AACGCTTTGG TCAACTGGAC 
AGCGGATACA TGAGCACCGA TTCAACGGAA CTGTACAAGC GAAATGTCTA CGATCGGTAT 
AATTTCAAGA TGCCGTGTGG ACGCGGACAG ATCATTCGGG TGGACACCAT CGAGGACAAC 
GAGGAGGAAA GCGGTCCGAA TGACAATCGG TTCGAGGACC TCGAGCACAT GGTCTCTCCA 
AGTCACAGTC GTCGATCGCC GGAAAACAAC GCAGTCGTTG CTTGTGATCT CAGCGATGCG 
GAATCTGATA AAACTCTGAC CCGTTCCCAT TCCCAAACGA ATTCCCAGGA GCGAAACGTG 
CGGGGCAGCA CCACAACAAT GTCATCCTAC TCCTCGGATG AAAATGGACG CGGACGAAGT 
ACCTGCTGTG GATCTTCGGA AACGGACGAG GAGACCAACG CGGAGGACAT GTGGGAGTCG 
GGGGCAGAGA GCATAGAAAC GCACTCTGTG CTCTACAAGA TGATTAGGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260889.1
CDS	529..5385
Translation	MRLQSLPLLLLLAVVTLPPATPTSAARPLTHPRARNHYPSEDLAEEPLMPASLLLEELQQTNGQLVNLTRQHDGDTFHTSGLSSACNADTCIGLSSGTASLVRSTGDGSGQGNDGLNPATSSLGGGQMELLTRRLKLQKGRGQVANIDASGETGCTCRCLPYQRAYREDLGICVDDIHECSLSPFVSGSSSEKIPFVFLPLKGQIIYPSREISFASIHTPVCAVTGAQYLGSNGWSDLRNPIDTDYPFRMFRDEGRSFLLWLGEADLRQKMQGRLIVVHLVCRDMTVALNASNHVKGEPLMPPRNVHSPCVAFRVNGSPVKYAHNVPEVFFQPANSTTLASTSDGMTMREYVVIGICSLLLGLIYVASVFLYLYMKKRKRHSSRHSLDNLTNDINYPKNDQVTYGAPFSRVGSIYSSNSMGLGNGNESNTRTSIGSLKEDMGIVKNNPLLQHFPQLSEREHNSGFASDISNSNSECEMEEKIKLSKSPKPSGGTDNAAMDVDDFLQDNECLPAENVAVIDELMSEEKLENLRAMVSGNVRKKLYFNPAYFEPHLLAEPPQAAIEFLQKIREVIAIAKYKMASKRYQPSLNIIPEEQHPESSSSSPMFNIPLQQSLAGKGMGDKRNSIEQWLQSVPNSDATPAPKTLDRPAPPTAKKPSSGSPNKGSLRKEISAPKERPPPPPMQKSKADEGNAKSSSPAPSGPHSSRSTGKSSPEQSPKPKTNVGSTYEGFSAFSVAANVYGFAETGGEIYNNPKFMLADSPAASLRSASSRSKSMKKRSEVLDQFAAANSTPTSLTSFDRQHYNMLRNMKAPEVIYDSLKREFAAHIPTPDYDSTIEKRIKDIYSSTQSSIPSVPTPDYSSLSRKSLKQFQPDSPIYRRKSPQYLIVDYETDSLERLEPSKRKSSHSSSSPSSDVSSQLSPSLSTALPLEEEMEISHTVYDRMNGFRKEGEMKKRLAAKHQHPHPGQQQRQKEAAARIKYDTPFRGSMTIEVEHEPPSDLERTDSDQFEPDTLDRKPKKQSFCDISAWDGHKPIDSDFSQISSLPDLSRQISPAPNQGIKPRTASFILRSSNSFRNLREIYESPLIAQDNIKYEKKVAQTPEEEGRILTLEAKHSRRQRLMETSPTHSVMDITKVNKTTITISSPKPADKAPPTRPKVNQHYCPPLVQSKRPLPPPPPNSSEHQAPDEDAYSLHSEITGTSEFENSCNNTMSSAISATTEERTHAKGSRSPKINAKIPEEDYEGMYNKKINSLRNDYSLTKYLNRRKSQREEVSKAGPDSPKDVAPEPEFGGSNKSVKEVDVAQFDALSISSRSNRSSSHLSERTKEMYRNAGVPVGIAYPQRAASSSGNASAGGNVQTVYRCEIEQAQLTAGMQIAIGLKDRAKKAKDLKNAWRKFVTMAANKFSPSQSPAPTYGSKTAAGFLEDDGIASIIEDAAQSAGMGQPGSQTYYEQRFGQLDSGYMSTDSTELYKRNVYDRYNFKMPCGRGQIIRVDTIEDNEEESGPNDNRFEDLEHMVSPSHSRRSPENNAVVACDLSDAESDKTLTRSHSQTNSQERNVRGSTTTMSSYSSDENGRGRSTCCGSSETDEETNAEDMWESGAESIETHSVLYKMIRKS

Target ORF information:

RefSeq Version	NM_001273960.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shavenoid (sha), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273960.1

ORF Insert Sequence:

ATGCGGCTCC AATCCTTACC GCTGCTCCTT CTGCTCGCTG TCGTCACCCT TCCGCCTGCC 
ACGCCCACTT CGGCTGCCCG ACCGCTTACA CACCCGAGGG CGAGGAACCA CTATCCCAGC 
GAAGATCTCG CCGAGGAGCC ACTAATGCCG GCCAGCTTGC TGCTCGAGGA ACTGCAGCAG 
ACCAACGGGC AGCTCGTCAA TCTGACACGA CAGCACGACG GCGATACCTT CCACACAAGC 
GGTTTGAGCA GCGCCTGCAA CGCGGACACC TGCATCGGAC TCTCAAGTGG CACAGCGTCG 
CTGGTCCGTT CGACCGGCGA TGGAAGCGGT CAAGGCAACG ATGGACTCAA TCCGGCGACC 
TCGTCTTTGG GCGGCGGTCA AATGGAGCTC CTGACCCGTC GTCTCAAGTT GCAGAAGGGT 
CGCGGTCAAG TGGCGAATAT AGATGCGAGC GGTGAGACTG GGTGCACCTG CCGATGTCTT 
CCCTATCAGC GGGCCTATCG CGAGGATCTG GGCATATGCG TGGACGACAT CCATGAGTGC 
TCCCTGTCGC CATTCGTGAG TGGTTCCTCC TCGGAAAAGA TACCCTTTGT CTTCCTGCCG 
CTCAAGGGTC AGATCATCTA TCCCAGCAGG GAGATCAGCT TTGCCAGCAT TCACACACCC 
GTTTGCGCGG TAACCGGAGC CCAGTACCTG GGTTCAAATG GCTGGTCGGA TCTGCGGAAC 
CCCATAGACA CCGACTACCC GTTTCGCATG TTCCGCGATG AGGGACGCAG CTTCCTCTTG 
TGGCTGGGTG AAGCTGATCT GCGCCAGAAG ATGCAGGGTC GCCTGATTGT GGTGCATCTG 
GTGTGTCGCG ACATGACGGT GGCCTTGAAT GCCAGCAATC ACGTCAAGGG TGAACCCCTC 
ATGCCGCCCA GGAATGTTCA CTCACCATGT GTGGCTTTCA GAGTGAATGG CAGTCCCGTA 
AAATATGCCC ACAATGTCCC CGAGGTCTTT TTCCAACCAG CCAACTCCAC CACTTTGGCC 
TCCACTTCGG ATGGCATGAC CATGCGTGAG TACGTGGTCA TTGGGATCTG TAGCCTGCTC 
TTGGGTCTCA TCTATGTGGC CTCAGTGTTT CTATATCTTT ACATGAAGAA ACGAAAGCGG 
CACAGCTCTC GTCATTCCCT AGACAATCTG ACAAACGACA TCAATTATCC TAAGAATGAT 
CAGGTTACGT ATGGAGCACC CTTTAGCCGA GTGGGTAGCA TATACTCCTC CAATAGCATG 
GGTCTGGGAA ATGGAAATGA GTCCAATACA CGTACAAGTA TAGGCAGTTT AAAGGAGGAT 
ATGGGCATTG TGAAAAATAA TCCATTGCTG CAGCATTTTC CACAGTTGAG TGAACGAGAA 
CACAATTCCG GGTTTGCCAG CGATATTTCA AATTCGAATT CTGAGTGTGA AATGGAGGAA 
AAGATCAAGC TCAGTAAGTC GCCGAAGCCT TCGGGTGGAA CAGATAACGC TGCAATGGAT 
GTGGATGACT TCCTGCAGGA CAACGAGTGT CTGCCCGCTG AAAATGTGGC CGTAATTGAT 
GAGCTCATGA GCGAGGAGAA GCTGGAGAAC TTGCGTGCCA TGGTCAGTGG TAATGTGCGC 
AAGAAGCTCT ACTTCAATCC CGCTTATTTT GAGCCTCATC TACTAGCGGA ACCGCCTCAA 
GCTGCCATTG AGTTCCTGCA GAAGATTCGC GAGGTCATCG CCATTGCCAA GTACAAAATG 
GCCTCAAAGC GTTACCAACC CTCTCTGAAC ATAATTCCGG AGGAGCAGCA TCCGGAGTCC 
AGTTCCAGTT CACCCATGTT CAATATTCCG CTGCAGCAGA GTCTGGCGGG GAAGGGAATG 
GGCGATAAGC GGAACAGCAT CGAGCAGTGG CTGCAAAGTG TACCAAACTC GGATGCGACT 
CCGGCACCGA AGACGCTCGA CCGACCTGCT CCACCGACTG CAAAGAAGCC ATCATCCGGA 
TCTCCCAACA AGGGCTCATT GAGAAAGGAG ATAAGTGCAC CCAAGGAGCG ACCACCACCA 
CCTCCAATGC AGAAATCAAA AGCAGATGAA GGTAATGCAA AGTCATCTTC TCCAGCGCCT 
TCGGGTCCAC ATTCCAGCCG AAGCACCGGA AAGAGCAGTC CAGAACAGTC ACCCAAGCCG 
AAGACAAATG TGGGTAGCAC TTATGAGGGA TTCTCTGCCT TTTCCGTGGC CGCCAATGTC 
TATGGATTTG CAGAGACCGG CGGGGAAATC TACAATAATC CCAAGTTCAT GCTGGCAGAC 
TCACCTGCGG CCTCATTGCG CAGTGCCTCC AGTCGCTCCA AATCGATGAA GAAGCGTAGC 
GAGGTTCTGG ATCAGTTCGC TGCCGCCAAC TCCACACCCA CCTCGCTCAC CTCCTTCGAT 
CGGCAGCACT ACAACATGCT GAGAAACATG AAAGCGCCGG AGGTCATCTA CGATTCCTTG 
AAGCGCGAGT TTGCAGCGCA TATTCCAACG CCTGATTATG ATAGCACCAT AGAGAAACGA 
ATCAAAGACA TCTATAGCAG TACGCAGAGT AGCATACCAT CGGTGCCCAC TCCGGATTAC 
AGCTCTCTCA GCCGTAAATC TCTTAAGCAG TTCCAACCCG ATTCCCCGAT TTATAGAAGG 
AAGTCCCCGC AGTATCTGAT TGTGGACTAC GAAACGGATA GTCTTGAACG TCTGGAACCC 
AGCAAACGCA AGAGTTCCCA CTCGTCCAGT TCACCGTCAT CCGATGTGAG TTCCCAATTG 
AGTCCAAGCT TGAGTACCGC TCTTCCGCTC GAGGAGGAGA TGGAGATCAG TCACACTGTA 
TACGATCGTA TGAACGGATT TCGCAAGGAG GGCGAGATGA AAAAGCGTTT GGCTGCAAAG 
CACCAACATC CACATCCTGG GCAGCAACAA AGGCAGAAGG AGGCGGCAGC TCGAATCAAA 
TATGACACGC CCTTTCGGGG AAGCATGACC ATCGAGGTGG AACATGAACC CCCTTCCGAT 
CTGGAGCGAA CGGATAGCGA TCAATTCGAA CCGGATACAC TGGATCGTAA GCCCAAGAAG 
CAGAGCTTCT GCGACATTTC AGCTTGGGAT GGGCATAAGC CAATCGACTC GGACTTTAGC 
CAGATCAGTT CTCTGCCGGA TTTGAGTCGG CAAATCAGTC CTGCACCCAA TCAGGGCATT 
AAACCAAGGA CCGCTTCCTT CATCCTTCGA TCCAGCAACT CATTTAGGAA CTTGAGAGAG 
ATCTATGAGT CGCCTTTAAT CGCTCAGGAC AACATCAAGT ACGAGAAGAA GGTTGCACAA 
ACACCGGAAG AGGAGGGGCG TATTCTCACC CTGGAGGCCA AGCACTCTCG TCGCCAGCGT 
CTGATGGAGA CGTCACCAAC CCACTCAGTG ATGGATATCA CAAAAGTAAA CAAGACCACT 
ATTACGATTT CATCACCGAA GCCAGCGGAT AAAGCTCCTC CTACTCGACC AAAGGTCAAT 
CAGCATTACT GCCCACCGTT GGTGCAAAGC AAACGACCAC TACCACCTCC TCCCCCAAAC 
TCATCGGAAC ATCAGGCTCC AGATGAAGAT GCCTACAGCC TGCACAGTGA GATCACAGGC 
ACCTCGGAGT TCGAGAACTC CTGCAACAAC ACCATGTCCA GTGCTATATC GGCAACCACC 
GAGGAGAGAA CTCATGCAAA GGGTTCCAGA TCCCCGAAAA TCAATGCTAA GATACCGGAA 
GAGGACTACG AAGGGATGTA CAACAAAAAG ATCAACAGCC TGCGCAACGA CTACAGTCTG 
ACGAAGTATC TCAATCGACG GAAAAGTCAG CGGGAGGAGG TTTCAAAAGC TGGTCCGGAT 
TCCCCAAAGG ACGTTGCCCC CGAGCCGGAG TTCGGTGGCA GCAATAAGAG CGTAAAGGAG 
GTGGATGTGG CCCAGTTCGA TGCGCTGTCC ATAAGCTCAC GCTCGAATCG GAGTAGCAGT 
CATCTTTCCG AGCGAACAAA AGAGATGTAC AGGAACGCCG GAGTTCCAGT GGGCATTGCC 
TATCCCCAAC GAGCGGCTAG TAGTTCCGGA AATGCTAGTG CCGGCGGAAA CGTTCAAACT 
GTGTATCGCT GTGAAATCGA ACAAGCCCAG CTCACGGCTG GAATGCAGAT TGCCATTGGA 
CTAAAGGATC GCGCCAAGAA AGCCAAGGAC CTGAAGAATG CGTGGCGAAA GTTTGTCACT 
ATGGCGGCCA ACAAGTTTAG TCCCAGCCAA AGTCCAGCAC CAACATATGG TAGCAAAACT 
GCTGCCGGGT TCCTCGAGGA TGACGGCATT GCCTCCATTA TCGAAGATGC AGCTCAGTCC 
GCTGGAATGG GACAACCGGG TAGCCAGACG TATTACGAGC AACGCTTTGG TCAACTGGAC 
AGCGGATACA TGAGCACCGA TTCAACGGAA CTGTACAAGC GAAATGTCTA CGATCGGTAT 
AATTTCAAGA TGCCGTGTGG ACGCGGACAG ATCATTCGGG TGGACACCAT CGAGGACAAC 
GAGGAGGAAA GCGGTCCGAA TGACAATCGG TTCGAGGACC TCGAGCACAT GGTCTCTCCA 
AGTCACAGTC GTCGATCGCC GGAAAACAAC GCAGTCGTTG CTTGTGATCT CAGCGATGCG 
GAATCTGATA AAACTCTGAC CCGTTCCCAT TCCCAAACGA ATTCCCAGGA GCGAAACGTG 
CGGGGCAGCA CCACAACAAT GTCATCCTAC TCCTCGGATG AAAATGGACG CGGACGAAGT 
ACCTGCTGTG GATCTTCGGA AACGGACGAG GAGACCAACG CGGAGGACAT GTGGGAGTCG 
GGGGCAGAGA GCATAGAAAC GCACTCTGTG CTCTACAAGA TGATTAGGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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