CG12744 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12744
Entrez Gene ID	36024
Full Name	CG12744 gene product from transcript CG12744-RA
Synonyms	Dmel\CG12744
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46C1-46C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165717.3	NP_724850.1	uncharacterized protein, isoform A
NM_136686.4	NP_610530.1	uncharacterized protein, isoform B
NM_136686.5	NP_610530.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG12744 maintained epithelial architecture and cell fate in enterocytes following an acute infectious challenge.
Title: The zinc finger protein CG12744 is essential for differentiation and regeneration after infection in the adult Drosophila midgut.

The following CG12744 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12744 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09411	NM_165717.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12744), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09411	NM_136686.5 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12744), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OFa09411	NM_136686.4	Drosophila melanogaster CG12744, transcript variant B (CG12744), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12744 ( NM_165717.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724850.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12744), mRNA.

Price & Availability↑

CloneID	OFa09411
Clone ID Related Accession (Same CDS sequence)	NM_165717.3 , NM_136686.4 , NM_136686.5
Accession Version	NM_165717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165717.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724850.1
CDS	234..716
Translation	METALAEVQLSCLLCEQTFDATEKLDEHLPTHFPQPVSTGQTCDICGRTMRSSLELHQHYKRYHEAHVPNTEGHFQCQLCDKVFLLQDHLKVHVKIEHATDGYQPDEKSLDWQHYSPRSLDTTNDYKLDPILCPPPKRKYPPRSPFFNPNLWLGADNCFM

Target ORF information:

RefSeq Version	NM_165717.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12744), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165717.3

ORF Insert Sequence:

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12744 ( NM_136686.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610530.1 Drosophila melanogaster CG12744, transcript variant B (CG12744), mRNA.

Price & Availability↑

CloneID	OFa09411
Clone ID Related Accession (Same CDS sequence)	NM_165717.3 , NM_136686.4 , NM_136686.5
Accession Version	NM_136686.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-13
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136686.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610530.1
CDS	167..649
Translation	METALAEVQLSCLLCEQTFDATEKLDEHLPTHFPQPVSTGQTCDICGRTMRSSLELHQHYKRYHEAHVPNTEGHFQCQLCDKVFLLQDHLKVHVKIEHATDGYQPDEKSLDWQHYSPRSLDTTNDYKLDPILCPPPKRKYPPRSPFFNPNLWLGADNCFM

Target ORF information:

RefSeq Version	NM_136686.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CG12744, transcript variant B (CG12744), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136686.4

ORF Insert Sequence:

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12744 ( NM_136686.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610530.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12744), mRNA.

Price & Availability↑

CloneID	OFa09411
Clone ID Related Accession (Same CDS sequence)	NM_165717.3 , NM_136686.4 , NM_136686.5
Accession Version	NM_136686.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 31, 2018 this sequence version replaced NM_136686.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610530.1
CDS	559..1041
Translation	METALAEVQLSCLLCEQTFDATEKLDEHLPTHFPQPVSTGQTCDICGRTMRSSLELHQHYKRYHEAHVPNTEGHFQCQLCDKVFLLQDHLKVHVKIEHATDGYQPDEKSLDWQHYSPRSLDTTNDYKLDPILCPPPKRKYPPRSPFFNPNLWLGADNCFM

Target ORF information:

RefSeq Version	NM_136686.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12744), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136686.5

ORF Insert Sequence:

ATGGAAACGG CGTTGGCTGA AGTGCAACTT TCCTGTCTGC TGTGCGAGCA GACTTTTGAT 
GCCACTGAGA AGCTGGACGA ACACCTCCCC ACCCACTTTC CGCAGCCTGT GAGCACTGGT 
CAAACATGCG ATATATGCGG GCGCACGATG CGATCCTCGC TGGAACTGCA CCAGCACTAT 
AAAAGATACC ATGAAGCCCA TGTGCCCAAC ACAGAGGGAC ACTTTCAATG CCAGCTCTGC 
GACAAGGTAT TCCTCCTCCA GGATCATCTC AAGGTACACG TGAAGATTGA GCACGCGACC 
GATGGCTACC AACCAGATGA GAAGTCCTTA GATTGGCAGC ACTACTCTCC CAGAAGTCTA 
GACACAACAA ACGATTATAA GCTGGATCCC ATCTTGTGTC CTCCACCAAA GAGGAAGTAT 
CCGCCGCGCT CCCCCTTCTT CAATCCGAAT CTCTGGCTAG GCGCTGACAA CTGTTTTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12744 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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