Vang cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vang
Entrez Gene ID	35922
Full Name	Van Gogh
Synonyms	CG8075,Dmel\CG8075,Stb,Stbm,Vang/Stbm,stb,stbm,stbm/vang,strabismus/Van Gogh,vang,vang/stbm,vangogh/strabismus
General protein information	Preferred Names Van Gogh Names CG8075 gene product from transcript CG8075-RA CG8075-PA CG8075-PB Van Gogh/Strabismus Van gogh VanGogh Vang-PA Vang-PB Vang-gogh sltrabismus strabismus van gogh van-gogh vang gogh
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A4-45A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_057829.4	NP_477177.1	Van gogh, isoform A
NM_001273890.2	NP_001260819.1	Van gogh, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VANGL1	NP_001192672.1
Mus musculus (house mouse)	Vangl1	NP_808213.2
Rattus norvegicus (Norway rat)	Vangl1	NP_001103054.1
Gallus gallus (chicken)	VANGL1	XP_004938247.1
Drosophila melanogaster (fruit fly)	Vang	NP_001260819.1
Homo sapiens (human)	VANGL1	NP_001165883.1
Caenorhabditis elegans (roundworm)	vang-1	NP_508500.1
Pan troglodytes (chimpanzee)	VANGL1	XP_003949557.1
Danio rerio (zebrafish)	vangl1	NP_991313.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012288	XP_320249.4
Canis lupus familiaris (dog)	VANGL1	XP_540251.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transmembrane proteins Frizzled and Flamingo form a stoichiometric nucleus in the complex, while the relative levels of the other four core proteins can vary independently.we show that robust cell polarization is achieved over a range of complex stoichiometries but is dependent on maintaining appropriate levels of the components Frizzled and Strabismus.
Title: Robust Asymmetric Localization of Planar Polarity Proteins Is Associated with Organization into Signalosome-like Domains of Variable Stoichiometry.

Vang phosphorylation occurs on conserved N-terminal serine/threonine residues, is mediated by CK1epsilon/Dco, and is critical for polarized membrane localization of Vang and other PCP proteins.
Title: Frizzled-Induced Van Gogh Phosphorylation by CK1? Promotes Asymmetric Localization of Core PCP Factors in Drosophila.

It physically interacts with and stabilizes Frizzled, and it mediates intercellular Frizzled-Van Gogh
Title: The Drosophila selectin furrowed mediates intercellular planar cell polarity interactions via frizzled stabilization.

Data indicate that Kermit interacts with Go and another PCP component Vang during planar cell polarity (PCP).
Title: Kermit interacts with G?o, Vang, and motor proteins in Drosophila planar cell polarity.

We propose that Stbm recruits farnesylated Pk into asymmetric complexes, but also promotes degradation of excess Pk that would otherwise perturb feedback amplification.
Title: Strabismus promotes recruitment and degradation of farnesylated prickle in Drosophila melanogaster planar polarity specification.

The following Vang gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vang cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09325	NM_057829.4 Latest version!	Drosophila melanogaster Van gogh, transcript variant A (Vang), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12233	NM_001273890.2 Latest version!	Drosophila melanogaster Van gogh, transcript variant B (Vang), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vang ( NM_057829.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477177.1 Drosophila melanogaster Van gogh, transcript variant A (Vang), mRNA.

Price & Availability↑

CloneID	OFa09325
Clone ID Related Accession (Same CDS sequence)	NM_057829.4
Accession Version	NM_057829.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Van gogh, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057829.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACG AATCCGTCAA GTCGGAACAC AGTGGACGCT CCAGACGCTC GCGGAATCAC 
AACAACAACG GCGGAGGGGG TGGCGGCGGA GGTGGTGGCG GAGGAGGCAG TGTCAACAAC 
GGCTATCACA GGGAGCGGGA CCGCTCCAGA CATTCCCACC GCAGCACACA CTCCTCGAAG 
TCGGCCAAGG GATTTCAACG TGGCGACATG GCACCGTACC AGACCAGCGT TAATATGACA 
GGAGACGGCA GCCACGATGG CCAGGAAGTC ATCGAGGTGC AAATCCTTCC GCAGGACGAG 
AACTGGGGCG AGAATACTAC CGCCGTCACG GGGAATACCT CGGAGCAAAG CATCTCAATG 
GAGGACATAA ACAACATGTG GCATCGTGAG AGCGACAAGG GCTTCAGCTT TGCGTGCCGC 
CGATACGTGG AGTCGAGCTT CTACTTCCTG CTGGGATGCG GAGCGTTTTT TTCGCCAGTG 
GCAATGGTGG TGATGCCGTA TGTGGGCTTC TTTCCGTCGG CCTTCGATCA TCCGGAAATA 
ACGCAAACTG TTCGCACCCA GCTGCTGGCC TGCAGTGAGC AGTGCAAGGG CCAACTGGTA 
TCGCTGGCCG CCCGGCTGCT GCTTCTGGCC ATCGGCCTGT GGGCAGTGTT CATGCGACGT 
ACATCGGCCA CCATGCCACG GATATTCCTA TATCGCGCTC TCGTCCTCCT TCTCGTCACC 
ATCTGCACGT TTGCTTACTG GCTCTTCTAC ATCGTGCAGG TCACAAACGG GGCCAAAATC 
GTTGTGGAGA CGGGCGGCGA TGCCGTGGAC TACAAGTCAC TGGTAGGCTA TGCCACCAAT 
TTTGTGGACA CTCTTCTATT TATTCACTAT GTGGCCGTGG TGTTACTAGA GCTGCGACAC 
CAGCAGCCTT GCTACTACAT CAAGATCATT CGCTCCCCGG ACGGCGTTTC GCGCTCCTAC 
ATGTTGGGTC AGCTGAGCAT CCAACGGGCG GCTGTGTGGG TGCTACAGCA CTACTACGTG 
GATTTCCCCA TATTTAACCC TTACCTGGAG CGCATCCCAA TATCGGTCTC CAAATCGCAG 
CGAAACAAGA TATCAAACAG TTTCAAGTAC TACGAAGTGG ATGGGGTGAG CAATTCGCAA 
CAGCAAAGCC AGAGTCGAGC AGTTCTAGCA GCCAACGCGC GGCGCCGAGA CTCCTCGCAT 
AACGAGCGCT TTTACGAAGA GCACGAGTAC GAACGTCGTG TGAAGAAACG GCGTGCCCGT 
CTTATAACCG CCGCCGAGGA GGCCTTCACC CACATCAAAC GCATTCATAA CGAACCGGCT 
CCGGCACTGC CGCTCGATCC GCAGGAGGCT GCCTCGGCGG TTTTTCCCTC GATGGCCAGG 
GCGCTGCAAA AATACTTACG GGTTACGCGT CAGCAGCCGC GGCATACCTT TGAGAGCATA 
CTGAAACATC TGGCGCACTG CCTAAAGCAT GATCTGTCGC CCCGCGCCTT CTTGGAACCC 
TACTTGACCG AGTCTCCGGT GATGCAGAGC GAGAAGGAGC GACGCTGGGT GCAGTCTTGG 
TCCCTGATCT GCGACGAGAT CGTCTCTCGG CCAATTGGCA ACGAATGCAC GTTCCAGTTA 
ATTCAGAACG ATGTCTCCCT AATGGTAACC GTACACAAGC TGCCGCACTT CAACCTGGCC 
GAGGAGGTTG TGGATCCCAA AAGCAACAAA TTTGTTCTTA AGTTGAACTC CGAAACATCC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477177.1
CDS	339..2084
Translation	MENESVKSEHSGRSRRSRNHNNNGGGGGGGGGGGGGSVNNGYHRERDRSRHSHRSTHSSKSAKGFQRGDMAPYQTSVNMTGDGSHDGQEVIEVQILPQDENWGENTTAVTGNTSEQSISMEDINNMWHRESDKGFSFACRRYVESSFYFLLGCGAFFSPVAMVVMPYVGFFPSAFDHPEITQTVRTQLLACSEQCKGQLVSLAARLLLLAIGLWAVFMRRTSATMPRIFLYRALVLLLVTICTFAYWLFYIVQVTNGAKIVVETGGDAVDYKSLVGYATNFVDTLLFIHYVAVVLLELRHQQPCYYIKIIRSPDGVSRSYMLGQLSIQRAAVWVLQHYYVDFPIFNPYLERIPISVSKSQRNKISNSFKYYEVDGVSNSQQQSQSRAVLAANARRRDSSHNERFYEEHEYERRVKKRRARLITAAEEAFTHIKRIHNEPAPALPLDPQEAASAVFPSMARALQKYLRVTRQQPRHTFESILKHLAHCLKHDLSPRAFLEPYLTESPVMQSEKERRWVQSWSLICDEIVSRPIGNECTFQLIQNDVSLMVTVHKLPHFNLAEEVVDPKSNKFVLKLNSETSV

Target ORF information:

RefSeq Version	NM_057829.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Van gogh, transcript variant A (Vang), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057829.4

ORF Insert Sequence:

ATGGAAAACG AATCCGTCAA GTCGGAACAC AGTGGACGCT CCAGACGCTC GCGGAATCAC 
AACAACAACG GCGGAGGGGG TGGCGGCGGA GGTGGTGGCG GAGGAGGCAG TGTCAACAAC 
GGCTATCACA GGGAGCGGGA CCGCTCCAGA CATTCCCACC GCAGCACACA CTCCTCGAAG 
TCGGCCAAGG GATTTCAACG TGGCGACATG GCACCGTACC AGACCAGCGT TAATATGACA 
GGAGACGGCA GCCACGATGG CCAGGAAGTC ATCGAGGTGC AAATCCTTCC GCAGGACGAG 
AACTGGGGCG AGAATACTAC CGCCGTCACG GGGAATACCT CGGAGCAAAG CATCTCAATG 
GAGGACATAA ACAACATGTG GCATCGTGAG AGCGACAAGG GCTTCAGCTT TGCGTGCCGC 
CGATACGTGG AGTCGAGCTT CTACTTCCTG CTGGGATGCG GAGCGTTTTT TTCGCCAGTG 
GCAATGGTGG TGATGCCGTA TGTGGGCTTC TTTCCGTCGG CCTTCGATCA TCCGGAAATA 
ACGCAAACTG TTCGCACCCA GCTGCTGGCC TGCAGTGAGC AGTGCAAGGG CCAACTGGTA 
TCGCTGGCCG CCCGGCTGCT GCTTCTGGCC ATCGGCCTGT GGGCAGTGTT CATGCGACGT 
ACATCGGCCA CCATGCCACG GATATTCCTA TATCGCGCTC TCGTCCTCCT TCTCGTCACC 
ATCTGCACGT TTGCTTACTG GCTCTTCTAC ATCGTGCAGG TCACAAACGG GGCCAAAATC 
GTTGTGGAGA CGGGCGGCGA TGCCGTGGAC TACAAGTCAC TGGTAGGCTA TGCCACCAAT 
TTTGTGGACA CTCTTCTATT TATTCACTAT GTGGCCGTGG TGTTACTAGA GCTGCGACAC 
CAGCAGCCTT GCTACTACAT CAAGATCATT CGCTCCCCGG ACGGCGTTTC GCGCTCCTAC 
ATGTTGGGTC AGCTGAGCAT CCAACGGGCG GCTGTGTGGG TGCTACAGCA CTACTACGTG 
GATTTCCCCA TATTTAACCC TTACCTGGAG CGCATCCCAA TATCGGTCTC CAAATCGCAG 
CGAAACAAGA TATCAAACAG TTTCAAGTAC TACGAAGTGG ATGGGGTGAG CAATTCGCAA 
CAGCAAAGCC AGAGTCGAGC AGTTCTAGCA GCCAACGCGC GGCGCCGAGA CTCCTCGCAT 
AACGAGCGCT TTTACGAAGA GCACGAGTAC GAACGTCGTG TGAAGAAACG GCGTGCCCGT 
CTTATAACCG CCGCCGAGGA GGCCTTCACC CACATCAAAC GCATTCATAA CGAACCGGCT 
CCGGCACTGC CGCTCGATCC GCAGGAGGCT GCCTCGGCGG TTTTTCCCTC GATGGCCAGG 
GCGCTGCAAA AATACTTACG GGTTACGCGT CAGCAGCCGC GGCATACCTT TGAGAGCATA 
CTGAAACATC TGGCGCACTG CCTAAAGCAT GATCTGTCGC CCCGCGCCTT CTTGGAACCC 
TACTTGACCG AGTCTCCGGT GATGCAGAGC GAGAAGGAGC GACGCTGGGT GCAGTCTTGG 
TCCCTGATCT GCGACGAGAT CGTCTCTCGG CCAATTGGCA ACGAATGCAC GTTCCAGTTA 
ATTCAGAACG ATGTCTCCCT AATGGTAACC GTACACAAGC TGCCGCACTT CAACCTGGCC 
GAGGAGGTTG TGGATCCCAA AAGCAACAAA TTTGTTCTTA AGTTGAACTC CGAAACATCC 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vang ( NM_001273890.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260819.1 Drosophila melanogaster Van gogh, transcript variant B (Vang), mRNA.

Price & Availability↑

CloneID	OFa12233
Clone ID Related Accession (Same CDS sequence)	NM_001273890.2
Accession Version	NM_001273890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Van gogh, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273890.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACG AATCCGTCAA GTCGGAACAC AGTGGACGCT CCAGACGCTC GCGGAATCAC 
AACAACAACG GCGGAGGGGG TGGCGGCGGA GGTGGTGGCG GAGGAGGCAG TGTCAACAAC 
GGCTATCACA GGGAGCGGGA CCGCTCCAGA CATTCCCACC GCAGCACACA CTCCTCGAAG 
TCGGCCAAGG GATTTCAACG TGGCGACATG GCACCGTACC AGACCAGCGT TAATATGACA 
GGAGACGGCA GCCACGATGG CCAGGAAGTC ATCGAGGTGC AAATCCTTCC GCAGGACGAG 
AACTGGGGCG AGAATACTAC CGCCGTCACG GGGAATACCT CGGAGCAAAG CATCTCAATG 
GAGGACATAA ACAACATGTG GCATCGTGAG AGCGACAAGG GCTTCAGCTT TGCGTGCCGC 
CGATACGTGG AGTCGAGCTT CTACTTCCTG CTGGGATGCG GAGCGTTTTT TTCGCCAGTG 
GCAATGGTGG TGATGCCGTA TGTGGGCTTC TTTCCGTCGG CCTTCGATCA TCCGGAAATA 
ACGCAAACTG TTCGCACCCA GCTGCTGGCC TGCAGTGAGC AGTGCAAGGG CCAACTGGTA 
TCGCTGGCCG CCCGGCTGCT GCTTCTGGCC ATCGGCCTGT GGGCAGTGTT CATGCGACGT 
ACATCGGCCA CCATGCCACG GATATTCCTA TATCGCGCTC TCGTCCTCCT TCTCGTCACC 
ATCTGCACGT TTGCTTACTG GCTCTTCTAC ATCGTGCAGG TCACAAACGG GGCCAAAATC 
GTTGTGGAGA CGGGCGGCGA TGCCGTGGAC TACAAGTCAC TGGTAGGCTA TGCCACCAAT 
TTTGTGGACA CTCTTCTATT TATTCACTAT GTGGCCGTGG TGTTACTAGA GCTGCGACAC 
CAGCAGCCTT GCTACTACAT CAAGATCATT CGCTCCCCGG ACGGCGTTTC GCGCTCCTAC 
ATGTTGGGTC AGCTGAGCAT CCAACGGGCG GCTGTGTGGG TGCTACAGCA CTACTACGTG 
GATTTCCCCA TATTTAACCC TTACCTGGAG CGCATCCCAA TATCGGTCTC CAAATCGCAG 
CGAAACAAGA TATCAAACAG TTTCAAGTAC TACGAAGTGG ATGGGGTGAG CAATTCGCAA 
CAGCAAAGCC AGAGTCGAGC AGTTCTAGCA GCCAACGCGC GGCGCCGAGA CTCCTCGCAT 
AACGAGCGCT TTTACGAAGA GCACGAGTAC GAACGTCGTG TGAAGAAACG GCGTGCCCGT 
CTTATAACCG CCGCCGAGGA GGCCTTCACC CACATCAAAC GCATTCATAA CGAACCGGCT 
CCGGCACTGC CGCTCGATCC GCAGGAGGCT GCCTCGGCGG TTTTTCCCTC GATGGCCAGG 
GCGCTGCAAA AATACTTACG GGTTACGCGT CAGCAGCCGC GGCATACCTT TGAGAGCATA 
CTGAAACATC TGGCGCACTG CCTAAAGCAT GATCTGTCGC CCCGCGCCTT CTTGGAACCC 
TACTTGACCG AGTCTCCGGT GATGCAGAGC GAGAAGGAGC GACGCTGGGT GCAGTCTTGG 
TCCCTGATCT GCGACGAGAT CGTCTCTCGG CCAATTGGCA ACGAATGCAC GTTCCAGTTA 
ATTCAGAACG ATGTCTCCCT AATGGTAACC GTACACAAGC TGCCGCACTT CAACCTGGCC 
GAGGAGGTTG TGGATCCCAA AAGCAACAAA TTTGTTCTTA AGTTGAACTC CGAAACATCC 
GTATGACACC ACCACAACGC CCCTCCAATC AACACTCCCA CTCATAGCAA CCAGACGCAA 
TCGTCCTACT TGCATGTGTT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260819.1
CDS	339..2165
Translation	MENESVKSEHSGRSRRSRNHNNNGGGGGGGGGGGGGSVNNGYHRERDRSRHSHRSTHSSKSAKGFQRGDMAPYQTSVNMTGDGSHDGQEVIEVQILPQDENWGENTTAVTGNTSEQSISMEDINNMWHRESDKGFSFACRRYVESSFYFLLGCGAFFSPVAMVVMPYVGFFPSAFDHPEITQTVRTQLLACSEQCKGQLVSLAARLLLLAIGLWAVFMRRTSATMPRIFLYRALVLLLVTICTFAYWLFYIVQVTNGAKIVVETGGDAVDYKSLVGYATNFVDTLLFIHYVAVVLLELRHQQPCYYIKIIRSPDGVSRSYMLGQLSIQRAAVWVLQHYYVDFPIFNPYLERIPISVSKSQRNKISNSFKYYEVDGVSNSQQQSQSRAVLAANARRRDSSHNERFYEEHEYERRVKKRRARLITAAEEAFTHIKRIHNEPAPALPLDPQEAASAVFPSMARALQKYLRVTRQQPRHTFESILKHLAHCLKHDLSPRAFLEPYLTESPVMQSEKERRWVQSWSLICDEIVSRPIGNECTFQLIQNDVSLMVTVHKLPHFNLAEEVVDPKSNKFVLKLNSETSV*HHHNAPPINTPTHSNQTQSSYLHVFV

Target ORF information:

RefSeq Version	NM_001273890.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Van gogh, transcript variant B (Vang), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273890.2

ORF Insert Sequence:

ATGGAAAACG AATCCGTCAA GTCGGAACAC AGTGGACGCT CCAGACGCTC GCGGAATCAC 
AACAACAACG GCGGAGGGGG TGGCGGCGGA GGTGGTGGCG GAGGAGGCAG TGTCAACAAC 
GGCTATCACA GGGAGCGGGA CCGCTCCAGA CATTCCCACC GCAGCACACA CTCCTCGAAG 
TCGGCCAAGG GATTTCAACG TGGCGACATG GCACCGTACC AGACCAGCGT TAATATGACA 
GGAGACGGCA GCCACGATGG CCAGGAAGTC ATCGAGGTGC AAATCCTTCC GCAGGACGAG 
AACTGGGGCG AGAATACTAC CGCCGTCACG GGGAATACCT CGGAGCAAAG CATCTCAATG 
GAGGACATAA ACAACATGTG GCATCGTGAG AGCGACAAGG GCTTCAGCTT TGCGTGCCGC 
CGATACGTGG AGTCGAGCTT CTACTTCCTG CTGGGATGCG GAGCGTTTTT TTCGCCAGTG 
GCAATGGTGG TGATGCCGTA TGTGGGCTTC TTTCCGTCGG CCTTCGATCA TCCGGAAATA 
ACGCAAACTG TTCGCACCCA GCTGCTGGCC TGCAGTGAGC AGTGCAAGGG CCAACTGGTA 
TCGCTGGCCG CCCGGCTGCT GCTTCTGGCC ATCGGCCTGT GGGCAGTGTT CATGCGACGT 
ACATCGGCCA CCATGCCACG GATATTCCTA TATCGCGCTC TCGTCCTCCT TCTCGTCACC 
ATCTGCACGT TTGCTTACTG GCTCTTCTAC ATCGTGCAGG TCACAAACGG GGCCAAAATC 
GTTGTGGAGA CGGGCGGCGA TGCCGTGGAC TACAAGTCAC TGGTAGGCTA TGCCACCAAT 
TTTGTGGACA CTCTTCTATT TATTCACTAT GTGGCCGTGG TGTTACTAGA GCTGCGACAC 
CAGCAGCCTT GCTACTACAT CAAGATCATT CGCTCCCCGG ACGGCGTTTC GCGCTCCTAC 
ATGTTGGGTC AGCTGAGCAT CCAACGGGCG GCTGTGTGGG TGCTACAGCA CTACTACGTG 
GATTTCCCCA TATTTAACCC TTACCTGGAG CGCATCCCAA TATCGGTCTC CAAATCGCAG 
CGAAACAAGA TATCAAACAG TTTCAAGTAC TACGAAGTGG ATGGGGTGAG CAATTCGCAA 
CAGCAAAGCC AGAGTCGAGC AGTTCTAGCA GCCAACGCGC GGCGCCGAGA CTCCTCGCAT 
AACGAGCGCT TTTACGAAGA GCACGAGTAC GAACGTCGTG TGAAGAAACG GCGTGCCCGT 
CTTATAACCG CCGCCGAGGA GGCCTTCACC CACATCAAAC GCATTCATAA CGAACCGGCT 
CCGGCACTGC CGCTCGATCC GCAGGAGGCT GCCTCGGCGG TTTTTCCCTC GATGGCCAGG 
GCGCTGCAAA AATACTTACG GGTTACGCGT CAGCAGCCGC GGCATACCTT TGAGAGCATA 
CTGAAACATC TGGCGCACTG CCTAAAGCAT GATCTGTCGC CCCGCGCCTT CTTGGAACCC 
TACTTGACCG AGTCTCCGGT GATGCAGAGC GAGAAGGAGC GACGCTGGGT GCAGTCTTGG 
TCCCTGATCT GCGACGAGAT CGTCTCTCGG CCAATTGGCA ACGAATGCAC GTTCCAGTTA 
ATTCAGAACG ATGTCTCCCT AATGGTAACC GTACACAAGC TGCCGCACTT CAACCTGGCC 
GAGGAGGTTG TGGATCCCAA AAGCAACAAA TTTGTTCTTA AGTTGAACTC CGAAACATCC 
GTATGACACC ACCACAACGC CCCTCCAATC AACACTCCCA CTCATAGCAA CCAGACGCAA 
TCGTCCTACT TGCATGTGTT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vang cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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