Spt cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Spt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa12823 NM_136579.5
Latest version!
Drosophila melanogaster spitting image (Spt), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa12823
    Clone ID Related Accession (Same CDS sequence) NM_136579.5
    Accession Version NM_136579.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product spitting image
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136579.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAAATTT TGCGAACGAA ACTAGAGGCC TGCCTGCTGA TACTGAACAG GAACAATGCA 
    GCAGCTGCAA ACTGGCTGAA TGACCTGTGC GCCGTGCTAT TGGAATTCGC TACTTTGGCC
    CACAGCTCCT TCTGCTCCCA GGGGCAGTCG GAGTGCTCGG AGCTGGTGTG CCTCTGCCTC
    AGCCAGATTA TGATCTGCAT TCGCCAAGTG GAGAACACCA TGAAACTAGA ATGCGGCACC
    ACAGTCTCGG TAAGTCATCA GTACTTCCTG GACCGCATTC GCTGGTGCCT GAAGCGCCTC
    ATGCATCTTT ACAGCGAAGA ATCGAGTTCC GAAGCAGTAG CTCCGGAGCG CTCGTTCCTT
    AAGCTGGTGG ATGGCGCATT GGATTGTTTG GCGCAGTTCA CCTCGCACAG TGAGGAAAAC
    ACCGATACCT CCAACTGTCC AAAGACTCCC AAGGAAGTCC TTCCACTCAG CCAGAAGTTG
    CGCGCCAACA TTGATTTGAT CTTGGGTCAA TCTCTGGGAT TCGCCAATGT GGCTTTGTCT
    CAGGACAAGA GGGCTCTCAG TGCCTTGTGC CAAAAGGTCA TCCGGGAGTG CAATGCCTTT
    CAGGAGGAGT GCAAGGAGTC ATTCAGTGCC AACTACCTGT CCACCCTTAA GCTGCGGGCC
    ATGACCATGG AACAGGCAAT TTACCAATTG GAGGACTTTA TCAACGATGC GCTTTTGCGC
    TTGGTATTCA CATGCTTCCT GGACTTCGAG AAGTTTTCGG TAGACAAAAT TAGAGCGCTG
    TTAAGGAACA GCCCAGATAA TGATGATCTG GCCGATGAGT TCATAGCGGA CTTTGATGTG
    AACATAGATC GCGCCACCCA GATAGGCATT TTTGCCATTT CCTTTGCGCC CAACTTGAAA
    ATGAAAACCA TAATGCGCAG CTGTCTGGCC TCCTTTGAAT CACTGGACAC CACTCTGATA
    CCTTCACTGC AGGCCCATGG TTCCGATTTG CACTCGGACA TCCTAGAGCA GCACTTTAAC
    GAGGAGGTGG CCAAGTTCAA GGCAGCGCTT CAGGAGATCA TCGACAGCCG CGCCCTCGTT
    GGTTGTTGCC TGGAGATACT GACCTCAGGG ATAAGTGCAA CGGAAAAGCT TTTCGATAAG
    CAGCGATTGG AGGACTTGGT TCAAATCTCT TTGCTGCTAG TCGAGCACTT TCAGCTGGAA
    GTCAACCGAA AGGGTCTAAG CGAAACGCAG GACCGATTGG GGGAAGAGTA CTACCAGCAG
    TTCATCCGGG TACTGCGCGA GTGCAAGGCT ATTTTGATGT GCGCATCCCA GGTGGAGCCG
    CAGAGAATCC TCAAGCGCTT CAAGATCTTG CGCACGATCT TGCGCAAATT GCACGGCTCC
    CTGGGAGCTG GTAAACAAGA GGAGGAACTT CCTATTCCCC TGGCTCCCCT TTTAGCTGAT
    GATACCAAGG GGGTAAATGA AGACGAAACC CATTTCACTG GCTATAAAAC AGCCCGAAGC
    GGCAGTATCC TCTACGACAC AGAGCGTAGG AATAGAGTAA CTAGACGAAC GGACAACAAG
    CTACCCACAA ATACCTTGGA CATTAAATCG GATAAGGCAA AACCCCATGG AAATGTTCTG
    AGAAGAGAAA GTTTACGGAC TGTCATGTTC AAGCGGCAGA ATATAGCTGA AAGCAGAAAG
    CTATACACCT CCATGAGTAA TCAGTCTACG GATCTACAAA TCACAGATAT ACTCGACCAA
    CTCACTGGAA TGTCCAATGG ATACTTTGAA TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_610423.3
    CDS107..1882
    Translation

    Target ORF information:

    RefSeq Version NM_136579.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster spitting image (Spt), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_136579.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAAATTT TGCGAACGAA ACTAGAGGCC TGCCTGCTGA TACTGAACAG GAACAATGCA 
    GCAGCTGCAA ACTGGCTGAA TGACCTGTGC GCCGTGCTAT TGGAATTCGC TACTTTGGCC
    CACAGCTCCT TCTGCTCCCA GGGGCAGTCG GAGTGCTCGG AGCTGGTGTG CCTCTGCCTC
    AGCCAGATTA TGATCTGCAT TCGCCAAGTG GAGAACACCA TGAAACTAGA ATGCGGCACC
    ACAGTCTCGG TAAGTCATCA GTACTTCCTG GACCGCATTC GCTGGTGCCT GAAGCGCCTC
    ATGCATCTTT ACAGCGAAGA ATCGAGTTCC GAAGCAGTAG CTCCGGAGCG CTCGTTCCTT
    AAGCTGGTGG ATGGCGCATT GGATTGTTTG GCGCAGTTCA CCTCGCACAG TGAGGAAAAC
    ACCGATACCT CCAACTGTCC AAAGACTCCC AAGGAAGTCC TTCCACTCAG CCAGAAGTTG
    CGCGCCAACA TTGATTTGAT CTTGGGTCAA TCTCTGGGAT TCGCCAATGT GGCTTTGTCT
    CAGGACAAGA GGGCTCTCAG TGCCTTGTGC CAAAAGGTCA TCCGGGAGTG CAATGCCTTT
    CAGGAGGAGT GCAAGGAGTC ATTCAGTGCC AACTACCTGT CCACCCTTAA GCTGCGGGCC
    ATGACCATGG AACAGGCAAT TTACCAATTG GAGGACTTTA TCAACGATGC GCTTTTGCGC
    TTGGTATTCA CATGCTTCCT GGACTTCGAG AAGTTTTCGG TAGACAAAAT TAGAGCGCTG
    TTAAGGAACA GCCCAGATAA TGATGATCTG GCCGATGAGT TCATAGCGGA CTTTGATGTG
    AACATAGATC GCGCCACCCA GATAGGCATT TTTGCCATTT CCTTTGCGCC CAACTTGAAA
    ATGAAAACCA TAATGCGCAG CTGTCTGGCC TCCTTTGAAT CACTGGACAC CACTCTGATA
    CCTTCACTGC AGGCCCATGG TTCCGATTTG CACTCGGACA TCCTAGAGCA GCACTTTAAC
    GAGGAGGTGG CCAAGTTCAA GGCAGCGCTT CAGGAGATCA TCGACAGCCG CGCCCTCGTT
    GGTTGTTGCC TGGAGATACT GACCTCAGGG ATAAGTGCAA CGGAAAAGCT TTTCGATAAG
    CAGCGATTGG AGGACTTGGT TCAAATCTCT TTGCTGCTAG TCGAGCACTT TCAGCTGGAA
    GTCAACCGAA AGGGTCTAAG CGAAACGCAG GACCGATTGG GGGAAGAGTA CTACCAGCAG
    TTCATCCGGG TACTGCGCGA GTGCAAGGCT ATTTTGATGT GCGCATCCCA GGTGGAGCCG
    CAGAGAATCC TCAAGCGCTT CAAGATCTTG CGCACGATCT TGCGCAAATT GCACGGCTCC
    CTGGGAGCTG GTAAACAAGA GGAGGAACTT CCTATTCCCC TGGCTCCCCT TTTAGCTGAT
    GATACCAAGG GGGTAAATGA AGACGAAACC CATTTCACTG GCTATAAAAC AGCCCGAAGC
    GGCAGTATCC TCTACGACAC AGAGCGTAGG AATAGAGTAA CTAGACGAAC GGACAACAAG
    CTACCCACAA ATACCTTGGA CATTAAATCG GATAAGGCAA AACCCCATGG AAATGTTCTG
    AGAAGAGAAA GTTTACGGAC TGTCATGTTC AAGCGGCAGA ATATAGCTGA AAGCAGAAAG
    CTATACACCT CCATGAGTAA TCAGTCTACG GATCTACAAA TCACAGATAT ACTCGACCAA
    CTCACTGGAA TGTCCAATGG ATACTTTGAA TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.