Dgk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgk
Entrez Gene ID	35738
Full Name	Diacyl glycerol kinase
Synonyms	CG12820,CG1535,CG18654,CG34361,DGKb,Dmel\CG34361,Dmel_CG12820,Dmel_CG18654,dDGK,dgk
General protein information	Preferred Names Diacyl glycerol kinase Names CG34361 gene product from transcript CG34361-RH CG34361-PE CG34361-PF CG34361-PG CG34361-PH CG34361-PI CG34361-PJ CG34361-PK Dgk-PE Dgk-PF Dgk-PG Dgk-PH Dgk-PI Dgk-PJ Dgk-PK diacyl glycerol kinase diacylglycerol kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E18-43F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165568.3	NP_724619.2	diacyl glycerol kinase, isoform H
NM_001273863.1	NP_001260792.1	diacyl glycerol kinase, isoform J
NM_206053.2	NP_995775.2	diacyl glycerol kinase, isoform I
NM_001103749.2	NP_001097219.1	diacyl glycerol kinase, isoform E
NM_001103750.3	NP_001097220.1	diacyl glycerol kinase, isoform F
NM_078930.4	NP_523654.2	diacyl glycerol kinase, isoform G
NM_001316408.1	NP_001303337.1	diacyl glycerol kinase, isoform K

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKB	NP_004071.1
Bos taurus (cattle)	DGKB	NP_001179827.1
Drosophila melanogaster (fruit fly)	Dgk	NP_001097220.1
Canis lupus familiaris (dog)	DGKB	XP_539445.3
Danio rerio (zebrafish)	dgkb	XP_003200112.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006762	XP_308982.4
Caenorhabditis elegans (roundworm)	dgk-3	NP_499031.3
Xenopus tropicalis (tropical clawed frog)	dgkb	NP_001096214.1
Pan troglodytes (chimpanzee)	DGKB	XP_001148960.1
	DGKB	XP_001105384.1
Rattus norvegicus (Norway rat)	Dgkb	NP_062177.1
Gallus gallus (chicken)	DGKB	XP_001235516.3
Mus musculus (house mouse)	Dgkb	NP_848796.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dgk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09206	NM_165568.3 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant H (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa09207	NM_001103750.3 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant F (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa09208	NM_078930.4 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant G (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa09209	NM_001103749.2 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant E (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa09210	NM_206053.2 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant I (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa12763	NM_001273863.1 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant J (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa41313	NM_001316408.1 Latest version!	Drosophila melanogaster diacyl glycerol kinase, transcript variant K (Dgk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgk ( NM_165568.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724619.2 Drosophila melanogaster diacyl glycerol kinase, transcript variant H (Dgk), mRNA.

Price & Availability↑

CloneID	OFa09206
Clone ID Related Accession (Same CDS sequence)	NM_165568.3
Accession Version	NM_165568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165568.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GGCTCCATAC CAAACATCAA TAGCATTGCC GAGCTGATGC CGCAGTGCAG CGGAGGCGGA 
GGCGGCATCG GTGGCACGGG GGGCGTGGCC GGGGCCGAAG GGCACGCACA AGCCCGCAGC 
AGCTTTGTGG ACAAAATCCA TGGCATCACG GATAAATTAC ACCACTCGCT GGGCGGACAT 
CTATCCCACG ATCCTAGCAA GACCGGCAGT GTCCATCCGA TGCTCACGGT GACGCCGAGT 
CCCTTGGCCA GCGGACCCAG CATGTTCCAG GCGAGCAATC CGGCCCGTCG CTCCGTGGAC 
TCCAGTCCCT CGCACTCGGC CACCAACCAC TCGCAGATGT CTCGCAACTC CTCGAAGAAG 
AGCAGCAACT CGGTTAATTG CAAAATTGAT GCAGTTTCAC GCATTTTGCC AGCAATTGGA 
CGGCATTGTC CTGGAAGTTT AGAGAGTGGG AGTGCACAGG CAGAAAAGAG GGCTGACATT 
AAGCTCCTCG CGCGAAAACT CTCCCATTTC GATCCGCTGA CACTCAAGGT CCCGCTGAAG 
GATGTCGTCT GCTATTTATC ACTGCTCGAG GCAGGACGAC CGGAGGACAA GCTGGAGTTC 
ATGTTCCGGC TGTACGACAC GGATAGCAAC GGAGTCCTGG ACACGGCCGA AATGGACGCC 
ATTGTTAACC AGATGATGGC CGTGGCCGAA TACCTCGGCT GGGATGTCAG CGAACTGCGT 
CCGATACTGC AAGAGATGAT GGTTGAAATC GACTACGATG CCGATGGCAC GGTGTCCTTG 
GACGAGTGGC AGCGAGGCGG GATGACTACG ATTCCACTGC TGGTGCTCCT GGGTGTCGAC 
AGCACCACGC TCAAGGAGGA TGGCATCCAC GTGTGGCGCC TGAAGCACTT CAGCAAGCCG 
GCGTACTGCA ATCTCTGCCT CAACATGTTG GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT 
GTGCTTTGCA AGTACACCGT CCATGAGCGA TGCGTGCAAC ATGCTCCCGC GTCCTGCATC 
ACCACCTACG TCAAGTCCAA GAAACCAAAG TGCGGCGGAG ACCTACTGCA TCACTGGGTG 
GAGGGCAACT GCTATGGACG GTGCTCCAAG TGCCGCAAAA GGATAAAGGC CTACCACGGA 
ATCACAGGAC TTACCTGCCG CTGGTGTCAC ATGATGCTCC ACAACCGCTG CGCCTCGTCG 
GTGAAGAAGG AGTGCACCTT GGGCGAATAC TCCGAGCTAA TTGTCCCGCC CACGGCCATC 
TGCCCGGCGG TACTCGACCG CCAGCGATCC GTGAATCAGG CTCACAAGGC CACGCACTTC 
CAAATCACGC CGCCGGACGA GCTGAGCTGC CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC 
GGCGGTCGCC AGGGGGATCG CATCCTGCGG AAGTTCCAGT ACATGCTCAA TCCGCGCCAG 
GTGTACGACC TGTCCAAGGG CGGGCCCAAG GAGGGACTCA CACTCTTCAA GGACCTGCCT 
CGCTTCAGGG TCATTTGCTG TGGCGGCGAC GGCACCGTCG GCTGGGTTTT GGAAGCCATG 
GACTCCATAG AGTTGGCCAG CCAGCCGGCC ATCGGAGTGA TTCCCCTGGG AACGGGCAAC 
GACCTGGCTC GCTGTCTCCG CTGGGGCGGT GGATACGAGG GCGAAAACAT CCCCAAACTG 
ATGGACAAGT TTCGAAGAGC CTCCACCGTC ATGCTGGACC GCTGGAGCAT CGAGGTGACC 
AATACTCCGC ACAGCGATGA CATGCGACCC AAGGTGACGC TGCACTCGAA CATGCAGAAG 
GTGATTGAGC TGTCGCAAAG TGTTGTGGTC GACAAATCGC TGATGGAACG CTTCGAGGAA 
ATCCAACGGC AGAGCAAGCA GGTGGCCACG TCCATGGGCA CGGCGGCCTC AAGCACATCG 
ATTATGATGG CCAGCAAGAC GGAAACGGAA ATGGAAACGA TGGCCACGAT GGAGTTCGGC 
AGCAGCACGA CCACCACCAA CAGAACGACC ACGACCAAGA GCATTTCGAT GTCCACGTTC 
GAGACGCAGT GCCTGCAACA GACCCTGCGG ACAGCGATGA GCAGCAGCAG CAGCAACACG 
AGCAGCGGCA GTCCGTGCAA CGGCAACCAG GATGCGGAAA CGGAAGTAGA TTCCCATGCC 
GCTGCCGCTG CCGATGTCCG GGAGAAGTCC GTGCCCAGGA GGTCGGGTGA AACGGAAAAG 
CAATCACTTG AGACGCTGCT GCTGCAGCAC AAACAGCAGA TGCAACAACA GCAGCAGCAA 
CAGCAGCAAG GAGTCACATC ACTAGCTGTC GAGGAAGCTG CAACAGCAAC GCCAGTCGGG 
AGTAATCAAA GCGATAATAG TAGCCAGCGC AATAAGCAAA ACAACATCCT TAAACAGCAA 
ATTACATTGT CATTGGACTT GTCCGACCAT GAGGACGAGC CCAAGGACGA TGGCGGTGGC 
GCTGGCGATG GTACCAAGAG CAACGGCAAC AGCATTCCGG CAACACCAGC CACACCAATC 
ACACCCACCA CTCCGAATGC CGCATCAAGT GTGCTGCAGC AACAACAGCA ACAGCATCTC 
CAGTTCGAGC AGCAACAGAA GCCCATCAAA GTGCAATCCG ACAAGGACTG CACGGTGCCC 
TACAACATAA TCAACAATTA TTTCTCCGTC GGCGTGGACG CCGCCATCTG CGTCAAGTTC 
CACCTGGAGC GTGAGAAGAA CCCGCACAAA TTCAACAGCC GCATGAAGAA CAAGCTGTGG 
TACTTCGAGT ATGCAACATC CGAGACATTT GCGGCATCCT GCAAGAATCT CCACGAGAGC 
ATCGAGATTG TGTGCGATGG CGTGGCCCTG GACTTGGCCA ACGGACCCCA CTTGCAGGGA 
GTGGCTCTGC TCAACATCCC GTACACACAC GGCGGTTCCA ACCTGTGGGG CGAGCACCTG 
TCTCAGAAAC GGATCCGCAA GAGTGCCGGC CCCTTCGGCA AGAGTAAGAA ACTACGCGCC 
GGCGACAAGG AGTTCTCCGC CACCAGCTTC AATTCCGTGG ACCTCTCGGT GGCCATTCAG 
GACTTTGGAG ATCGGCTCAT CGAGGTAATT GGGCTGGAGA ACTGCCTGCA CATGGGCCAG 
GTGAGGACTG GACTTCGCGC GTCGGGACGC CGCCTGGCCC AGTGCAGCGA AGTGATCATC 
AAGACGAAGA AAACATTTCC CATGCAGATA GATGGCGAGC CCTGGATGCA GATGCCCTGC 
ACGATCAAGG TGACCCACAA GAACCAGGTG CCCATGCTGA TGGCACCGCG GTCGGAAAAG 
GGACGCGGAT TCTTCAACCT GCTGTGCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724619.2
CDS	579..4271
Translation	MNIGIAAPKWDKLSPREFLQLQELASYSTRKLQDVLREFSSPSAASTPKCIPDGDIDFDGFRRFLDAFLDCEAPLDLAKHLFVSFLKPNVTQAQLHGRALNQMAAISSTAACAPVTSHTKGSIPNINSIAELMPQCSGGGGGIGGTGGVAGAEGHAQARSSFVDKIHGITDKLHHSLGGHLSHDPSKTGSVHPMLTVTPSPLASGPSMFQASNPARRSVDSSPSHSATNHSQMSRNSSKKSSNSVNCKIDAVSRILPAIGRHCPGSLESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_165568.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant H (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165568.3

ORF Insert Sequence:

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GGCTCCATAC CAAACATCAA TAGCATTGCC GAGCTGATGC CGCAGTGCAG CGGAGGCGGA 
GGCGGCATCG GTGGCACGGG GGGCGTGGCC GGGGCCGAAG GGCACGCACA AGCCCGCAGC 
AGCTTTGTGG ACAAAATCCA TGGCATCACG GATAAATTAC ACCACTCGCT GGGCGGACAT 
CTATCCCACG ATCCTAGCAA GACCGGCAGT GTCCATCCGA TGCTCACGGT GACGCCGAGT 
CCCTTGGCCA GCGGACCCAG CATGTTCCAG GCGAGCAATC CGGCCCGTCG CTCCGTGGAC 
TCCAGTCCCT CGCACTCGGC CACCAACCAC TCGCAGATGT CTCGCAACTC CTCGAAGAAG 
AGCAGCAACT CGGTTAATTG CAAAATTGAT GCAGTTTCAC GCATTTTGCC AGCAATTGGA 
CGGCATTGTC CTGGAAGTTT AGAGAGTGGG AGTGCACAGG CAGAAAAGAG GGCTGACATT 
AAGCTCCTCG CGCGAAAACT CTCCCATTTC GATCCGCTGA CACTCAAGGT CCCGCTGAAG 
GATGTCGTCT GCTATTTATC ACTGCTCGAG GCAGGACGAC CGGAGGACAA GCTGGAGTTC 
ATGTTCCGGC TGTACGACAC GGATAGCAAC GGAGTCCTGG ACACGGCCGA AATGGACGCC 
ATTGTTAACC AGATGATGGC CGTGGCCGAA TACCTCGGCT GGGATGTCAG CGAACTGCGT 
CCGATACTGC AAGAGATGAT GGTTGAAATC GACTACGATG CCGATGGCAC GGTGTCCTTG 
GACGAGTGGC AGCGAGGCGG GATGACTACG ATTCCACTGC TGGTGCTCCT GGGTGTCGAC 
AGCACCACGC TCAAGGAGGA TGGCATCCAC GTGTGGCGCC TGAAGCACTT CAGCAAGCCG 
GCGTACTGCA ATCTCTGCCT CAACATGTTG GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT 
GTGCTTTGCA AGTACACCGT CCATGAGCGA TGCGTGCAAC ATGCTCCCGC GTCCTGCATC 
ACCACCTACG TCAAGTCCAA GAAACCAAAG TGCGGCGGAG ACCTACTGCA TCACTGGGTG 
GAGGGCAACT GCTATGGACG GTGCTCCAAG TGCCGCAAAA GGATAAAGGC CTACCACGGA 
ATCACAGGAC TTACCTGCCG CTGGTGTCAC ATGATGCTCC ACAACCGCTG CGCCTCGTCG 
GTGAAGAAGG AGTGCACCTT GGGCGAATAC TCCGAGCTAA TTGTCCCGCC CACGGCCATC 
TGCCCGGCGG TACTCGACCG CCAGCGATCC GTGAATCAGG CTCACAAGGC CACGCACTTC 
CAAATCACGC CGCCGGACGA GCTGAGCTGC CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC 
GGCGGTCGCC AGGGGGATCG CATCCTGCGG AAGTTCCAGT ACATGCTCAA TCCGCGCCAG 
GTGTACGACC TGTCCAAGGG CGGGCCCAAG GAGGGACTCA CACTCTTCAA GGACCTGCCT 
CGCTTCAGGG TCATTTGCTG TGGCGGCGAC GGCACCGTCG GCTGGGTTTT GGAAGCCATG 
GACTCCATAG AGTTGGCCAG CCAGCCGGCC ATCGGAGTGA TTCCCCTGGG AACGGGCAAC 
GACCTGGCTC GCTGTCTCCG CTGGGGCGGT GGATACGAGG GCGAAAACAT CCCCAAACTG 
ATGGACAAGT TTCGAAGAGC CTCCACCGTC ATGCTGGACC GCTGGAGCAT CGAGGTGACC 
AATACTCCGC ACAGCGATGA CATGCGACCC AAGGTGACGC TGCACTCGAA CATGCAGAAG 
GTGATTGAGC TGTCGCAAAG TGTTGTGGTC GACAAATCGC TGATGGAACG CTTCGAGGAA 
ATCCAACGGC AGAGCAAGCA GGTGGCCACG TCCATGGGCA CGGCGGCCTC AAGCACATCG 
ATTATGATGG CCAGCAAGAC GGAAACGGAA ATGGAAACGA TGGCCACGAT GGAGTTCGGC 
AGCAGCACGA CCACCACCAA CAGAACGACC ACGACCAAGA GCATTTCGAT GTCCACGTTC 
GAGACGCAGT GCCTGCAACA GACCCTGCGG ACAGCGATGA GCAGCAGCAG CAGCAACACG 
AGCAGCGGCA GTCCGTGCAA CGGCAACCAG GATGCGGAAA CGGAAGTAGA TTCCCATGCC 
GCTGCCGCTG CCGATGTCCG GGAGAAGTCC GTGCCCAGGA GGTCGGGTGA AACGGAAAAG 
CAATCACTTG AGACGCTGCT GCTGCAGCAC AAACAGCAGA TGCAACAACA GCAGCAGCAA 
CAGCAGCAAG GAGTCACATC ACTAGCTGTC GAGGAAGCTG CAACAGCAAC GCCAGTCGGG 
AGTAATCAAA GCGATAATAG TAGCCAGCGC AATAAGCAAA ACAACATCCT TAAACAGCAA 
ATTACATTGT CATTGGACTT GTCCGACCAT GAGGACGAGC CCAAGGACGA TGGCGGTGGC 
GCTGGCGATG GTACCAAGAG CAACGGCAAC AGCATTCCGG CAACACCAGC CACACCAATC 
ACACCCACCA CTCCGAATGC CGCATCAAGT GTGCTGCAGC AACAACAGCA ACAGCATCTC 
CAGTTCGAGC AGCAACAGAA GCCCATCAAA GTGCAATCCG ACAAGGACTG CACGGTGCCC 
TACAACATAA TCAACAATTA TTTCTCCGTC GGCGTGGACG CCGCCATCTG CGTCAAGTTC 
CACCTGGAGC GTGAGAAGAA CCCGCACAAA TTCAACAGCC GCATGAAGAA CAAGCTGTGG 
TACTTCGAGT ATGCAACATC CGAGACATTT GCGGCATCCT GCAAGAATCT CCACGAGAGC 
ATCGAGATTG TGTGCGATGG CGTGGCCCTG GACTTGGCCA ACGGACCCCA CTTGCAGGGA 
GTGGCTCTGC TCAACATCCC GTACACACAC GGCGGTTCCA ACCTGTGGGG CGAGCACCTG 
TCTCAGAAAC GGATCCGCAA GAGTGCCGGC CCCTTCGGCA AGAGTAAGAA ACTACGCGCC 
GGCGACAAGG AGTTCTCCGC CACCAGCTTC AATTCCGTGG ACCTCTCGGT GGCCATTCAG 
GACTTTGGAG ATCGGCTCAT CGAGGTAATT GGGCTGGAGA ACTGCCTGCA CATGGGCCAG 
GTGAGGACTG GACTTCGCGC GTCGGGACGC CGCCTGGCCC AGTGCAGCGA AGTGATCATC 
AAGACGAAGA AAACATTTCC CATGCAGATA GATGGCGAGC CCTGGATGCA GATGCCCTGC 
ACGATCAAGG TGACCCACAA GAACCAGGTG CCCATGCTGA TGGCACCGCG GTCGGAAAAG 
GGACGCGGAT TCTTCAACCT GCTGTGCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_001103750.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097220.1 Drosophila melanogaster diacyl glycerol kinase, transcript variant F (Dgk), mRNA.

Price & Availability↑

CloneID	OFa09207
Clone ID Related Accession (Same CDS sequence)	NM_001103750.3
Accession Version	NM_001103750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103750.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGAA ATCCCAAATG CATCACCACC AGGCCACGCA CTTCCAAATC 
ACGCCGCCGG ACGAGCTGAG CTGCCCGCTG CTGGTGTTCG TCAATCCGAA GAGCGGCGGT 
CGCCAGGGGG ATCGCATCCT GCGGAAGTTC CAGTACATGC TCAATCCGCG CCAGGTGTAC 
GACCTGTCCA AGGGCGGGCC CAAGGAGGGA CTCACACTCT TCAAGGACCT GCCTCGCTTC 
AGGGTCATTT GCTGTGGCGG CGACGGCACC GTCGGCTGGG TTTTGGAAGC CATGGACTCC 
ATAGAGTTGG CCAGCCAGCC GGCCATCGGA GTGATTCCCC TGGGAACGGG CAACGACCTG 
GCTCGCTGTC TCCGCTGGGG CGGTGGATAC GAGGGCGAAA ACATCCCCAA ACTGATGGAC 
AAGTTTCGAA GAGCCTCCAC CGTCATGCTG GACCGCTGGA GCATCGAGGT GACCAATACT 
CCGCACAGCG ATGACATGCG ACCCAAGGTG ACGCTGCACT CGAACATGCA GAAGGTGATT 
GAGCTGTCGC AAAGTGTTGT GGTCGACAAA TCGCTGATGG AACGCTTCGA GGAAATCCAA 
CGGCAGAGCA AGCAGGTGGC CACGTCCATG GGCACGGCGG CCTCAAGCAC ATCGATTATG 
ATGGCCAGCA AGACGGAAAC GGAAATGGAA ACGATGGCCA CGATGGAGTT CGGCAGCAGC 
ACGACCACCA CCAACAGAAC GACCACGACC AAGAGCATTT CGATGTCCAC GTTCGAGACG 
CAGTGCCTGC AACAGACCCT GCGGACAGCG ATGAGCAGCA GCAGCAGCAA CACGAGCAGC 
GGCAGTCCGT GCAACGGCAA CCAGGATGCG GAAACGGAAG TAGATTCCCA TGCCGCTGCC 
GCTGCCGATG TCCGGGAGAA GTCCGTGCCC AGGAGGTCGG GTGAAACGGA AAAGCAATCA 
CTTGAGACGC TGCTGCTGCA GCACAAACAG CAGATGCAAC AACAGCAGCA GCAACAGCAG 
CAAGGAGTCA CATCACTAGC TGTCGAGGAA GCTGCAACAG CAACGCCAGT CGGGAGTAAT 
CAAAGCGATA ATAGTAGCCA GCGCAATAAG CAAAACAACA TCCTTAAACA GCAAATTACA 
TTGTCATTGG ACTTGTCCGA CCATGAGGAC GAGCCCAAGG ACGATGGCGG TGGCGCTGGC 
GATGGTACCA AGAGCAACGG CAACAGCATT CCGGCAACAC CAGCCACACC AATCACACCC 
ACCACTCCGA ATGCCGCATC AAGTGTGCTG CAGCAACAAC AGCAACAGCA TCTCCAGTTC 
GAGCAGCAAC AGAAGCCCAT CAAAGTGCAA TCCGACAAGG ACTGCACGGT GCCCTACAAC 
ATAATCAACA ATTATTTCTC CGTCGGCGTG GACGCCGCCA TCTGCGTCAA GTTCCACCTG 
GAGCGTGAGA AGAACCCGCA CAAATTCAAC AGCCGCATGA AGAACAAGCT GTGGTACTTC 
GAGTATGCAA CATCCGAGAC ATTTGCGGCA TCCTGCAAGA ATCTCCACGA GAGCATCGAG 
ATTGTGTGCG ATGGCGTGGC CCTGGACTTG GCCAACGGAC CCCACTTGCA GGGAGTGGCT 
CTGCTCAACA TCCCGTACAC ACACGGCGGT TCCAACCTGT GGGGCGAGCA CCTGTCTCAG 
AAACGGATCC GCAAGAGTGC CGGCCCCTTC GGCAAGAGTA AGAAACTACG CGCCGGCGAC 
AAGGAGTTCT CCGCCACCAG CTTCAATTCC GTGGACCTCT CGGTGGCCAT TCAGGACTTT 
GGAGATCGGC TCATCGAGGT AATTGGGCTG GAGAACTGCC TGCACATGGG CCAGGTGAGG 
ACTGGACTTC GCGCGTCGGG ACGCCGCCTG GCCCAGTGCA GCGAAGTGAT CATCAAGACG 
AAGAAAACAT TTCCCATGCA GATAGATGGC GAGCCCTGGA TGCAGATGCC CTGCACGATC 
AAGGTGACCC ACAAGAACCA GGTGCCCATG CTGATGGCAC CGCGGTCGGA AAAGGGACGC 
GGATTCTTCA ACCTGCTGTG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097220.1
CDS	579..3905
Translation	MNIGIAAPKWDKLSPREFLQLQELASYSTRKLQDVLREFSSPSAASTPKCIPDGDIDFDGFRRFLDAFLDCEAPLDLAKHLFVSFLKPNVTQAQLHGRALNQMAAISSTAACAPVTSHTKAVSRILPAIGRHCPGSLESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKKSQMHHHQATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_001103750.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant F (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103750.3

ORF Insert Sequence:

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGAA ATCCCAAATG CATCACCACC AGGCCACGCA CTTCCAAATC 
ACGCCGCCGG ACGAGCTGAG CTGCCCGCTG CTGGTGTTCG TCAATCCGAA GAGCGGCGGT 
CGCCAGGGGG ATCGCATCCT GCGGAAGTTC CAGTACATGC TCAATCCGCG CCAGGTGTAC 
GACCTGTCCA AGGGCGGGCC CAAGGAGGGA CTCACACTCT TCAAGGACCT GCCTCGCTTC 
AGGGTCATTT GCTGTGGCGG CGACGGCACC GTCGGCTGGG TTTTGGAAGC CATGGACTCC 
ATAGAGTTGG CCAGCCAGCC GGCCATCGGA GTGATTCCCC TGGGAACGGG CAACGACCTG 
GCTCGCTGTC TCCGCTGGGG CGGTGGATAC GAGGGCGAAA ACATCCCCAA ACTGATGGAC 
AAGTTTCGAA GAGCCTCCAC CGTCATGCTG GACCGCTGGA GCATCGAGGT GACCAATACT 
CCGCACAGCG ATGACATGCG ACCCAAGGTG ACGCTGCACT CGAACATGCA GAAGGTGATT 
GAGCTGTCGC AAAGTGTTGT GGTCGACAAA TCGCTGATGG AACGCTTCGA GGAAATCCAA 
CGGCAGAGCA AGCAGGTGGC CACGTCCATG GGCACGGCGG CCTCAAGCAC ATCGATTATG 
ATGGCCAGCA AGACGGAAAC GGAAATGGAA ACGATGGCCA CGATGGAGTT CGGCAGCAGC 
ACGACCACCA CCAACAGAAC GACCACGACC AAGAGCATTT CGATGTCCAC GTTCGAGACG 
CAGTGCCTGC AACAGACCCT GCGGACAGCG ATGAGCAGCA GCAGCAGCAA CACGAGCAGC 
GGCAGTCCGT GCAACGGCAA CCAGGATGCG GAAACGGAAG TAGATTCCCA TGCCGCTGCC 
GCTGCCGATG TCCGGGAGAA GTCCGTGCCC AGGAGGTCGG GTGAAACGGA AAAGCAATCA 
CTTGAGACGC TGCTGCTGCA GCACAAACAG CAGATGCAAC AACAGCAGCA GCAACAGCAG 
CAAGGAGTCA CATCACTAGC TGTCGAGGAA GCTGCAACAG CAACGCCAGT CGGGAGTAAT 
CAAAGCGATA ATAGTAGCCA GCGCAATAAG CAAAACAACA TCCTTAAACA GCAAATTACA 
TTGTCATTGG ACTTGTCCGA CCATGAGGAC GAGCCCAAGG ACGATGGCGG TGGCGCTGGC 
GATGGTACCA AGAGCAACGG CAACAGCATT CCGGCAACAC CAGCCACACC AATCACACCC 
ACCACTCCGA ATGCCGCATC AAGTGTGCTG CAGCAACAAC AGCAACAGCA TCTCCAGTTC 
GAGCAGCAAC AGAAGCCCAT CAAAGTGCAA TCCGACAAGG ACTGCACGGT GCCCTACAAC 
ATAATCAACA ATTATTTCTC CGTCGGCGTG GACGCCGCCA TCTGCGTCAA GTTCCACCTG 
GAGCGTGAGA AGAACCCGCA CAAATTCAAC AGCCGCATGA AGAACAAGCT GTGGTACTTC 
GAGTATGCAA CATCCGAGAC ATTTGCGGCA TCCTGCAAGA ATCTCCACGA GAGCATCGAG 
ATTGTGTGCG ATGGCGTGGC CCTGGACTTG GCCAACGGAC CCCACTTGCA GGGAGTGGCT 
CTGCTCAACA TCCCGTACAC ACACGGCGGT TCCAACCTGT GGGGCGAGCA CCTGTCTCAG 
AAACGGATCC GCAAGAGTGC CGGCCCCTTC GGCAAGAGTA AGAAACTACG CGCCGGCGAC 
AAGGAGTTCT CCGCCACCAG CTTCAATTCC GTGGACCTCT CGGTGGCCAT TCAGGACTTT 
GGAGATCGGC TCATCGAGGT AATTGGGCTG GAGAACTGCC TGCACATGGG CCAGGTGAGG 
ACTGGACTTC GCGCGTCGGG ACGCCGCCTG GCCCAGTGCA GCGAAGTGAT CATCAAGACG 
AAGAAAACAT TTCCCATGCA GATAGATGGC GAGCCCTGGA TGCAGATGCC CTGCACGATC 
AAGGTGACCC ACAAGAACCA GGTGCCCATG CTGATGGCAC CGCGGTCGGA AAAGGGACGC 
GGATTCTTCA ACCTGCTGTG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_078930.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523654.2 Drosophila melanogaster diacyl glycerol kinase, transcript variant G (Dgk), mRNA.

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CloneID	OFa09208
Clone ID Related Accession (Same CDS sequence)	NM_078930.4
Accession Version	NM_078930.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078930.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATGGG AACTTCGTTA TGGCTGGCAA ATTGTCACGT TCCGAACAAA TTGCGCTACT 
GCTGCCGCCG TTGCATGCCA CCAACTTTTG TGTCCTGTGT CTTTGACAAG TGCAGTGGAG 
ATAATGCCGA ATTCCTTTCC GCCAGAGGGT CGTCGGCGGG AGCGAAGGTC GGGAAACTTG 
GTCCTTCGAG CCGGATGCTG CTGCATCGGC AAATACTTTC TCCACAACCG CTGCGCCTCG 
TCGGTGAAGA AGGAGTGCAC CTTGGGCGAA TACTCCGAGC TAATTGTCCC GCCCACGGCC 
ATCTGCCCGG CGGTACTCGA CCGCCAGCGA TCCGTGAATC AGGCTCACAA GGCCACGCAC 
TTCCAAATCA CGCCGCCGGA CGAGCTGAGC TGCCCGCTGC TGGTGTTCGT CAATCCGAAG 
AGCGGCGGTC GCCAGGGGGA TCGCATCCTG CGGAAGTTCC AGTACATGCT CAATCCGCGC 
CAGGTGTACG ACCTGTCCAA GGGCGGGCCC AAGGAGGGAC TCACACTCTT CAAGGACCTG 
CCTCGCTTCA GGGTCATTTG CTGTGGCGGC GACGGCACCG TCGGCTGGGT TTTGGAAGCC 
ATGGACTCCA TAGAGTTGGC CAGCCAGCCG GCCATCGGAG TGATTCCCCT GGGAACGGGC 
AACGACCTGG CTCGCTGTCT CCGCTGGGGC GGTGGATACG AGGGCGAAAA CATCCCCAAA 
CTGATGGACA AGTTTCGAAG AGCCTCCACC GTCATGCTGG ACCGCTGGAG CATCGAGGTG 
ACCAATACTC CGCACAGCGA TGACATGCGA CCCAAGGTGA CGCTGCACTC GAACATGCAG 
AAGGTGATTG AGCTGTCGCA AAGTGTTGTG GTCGACAAAT CGCTGATGGA ACGCTTCGAG 
GAAATCCAAC GGCAGAGCAA GCAGGTGGCC ACGTCCATGG GCACGGCGGC CTCAAGCACA 
TCGATTATGA TGGCCAGCAA GACGGAAACG GAAATGGAAA CGATGGCCAC GATGGAGTTC 
GGCAGCAGCA CGACCACCAC CAACAGAACG ACCACGACCA AGAGCATTTC GATGTCCACG 
TTCGAGACGC AGTGCCTGCA ACAGACCCTG CGGACAGCGA TGAGCAGCAG CAGCAGCAAC 
ACGAGCAGCG GCAGTCCGTG CAACGGCAAC CAGGATGCGG AAACGGAAGT AGATTCCCAT 
GCCGCTGCCG CTGCCGATGT CCGGGAGAAG TCCGTGCCCA GGAGGTCGGG TGAAACGGAA 
AAGCAATCAC TTGAGACGCT GCTGCTGCAG CACAAACAGC AGATGCAACA ACAGCAGCAG 
CAACAGCAGC AAGGAGTCAC ATCACTAGCT GTCGAGGAAG CTGCAACAGC AACGCCAGTC 
GGGAGTAATC AAAGCGATAA TAGTAGCCAG CGCAATAAGC AAAACAACAT CCTTAAACAG 
CAAATTACAT TGTCATTGGA CTTGTCCGAC CATGAGGACG AGCCCAAGGA CGATGGCGGT 
GGCGCTGGCG ATGGTACCAA GAGCAACGGC AACAGCATTC CGGCAACACC AGCCACACCA 
ATCACACCCA CCACTCCGAA TGCCGCATCA AGTGTGCTGC AGCAACAACA GCAACAGCAT 
CTCCAGTTCG AGCAGCAACA GAAGCCCATC AAAGTGCAAT CCGACAAGGA CTGCACGGTG 
CCCTACAACA TAATCAACAA TTATTTCTCC GTCGGCGTGG ACGCCGCCAT CTGCGTCAAG 
TTCCACCTGG AGCGTGAGAA GAACCCGCAC AAATTCAACA GCCGCATGAA GAACAAGCTG 
TGGTACTTCG AGTATGCAAC ATCCGAGACA TTTGCGGCAT CCTGCAAGAA TCTCCACGAG 
AGCATCGAGA TTGTGTGCGA TGGCGTGGCC CTGGACTTGG CCAACGGACC CCACTTGCAG 
GGAGTGGCTC TGCTCAACAT CCCGTACACA CACGGCGGTT CCAACCTGTG GGGCGAGCAC 
CTGTCTCAGA AACGGATCCG CAAGAGTGCC GGCCCCTTCG GCAAGAGTAA GAAACTACGC 
GCCGGCGACA AGGAGTTCTC CGCCACCAGC TTCAATTCCG TGGACCTCTC GGTGGCCATT 
CAGGACTTTG GAGATCGGCT CATCGAGGTA ATTGGGCTGG AGAACTGCCT GCACATGGGC 
CAGGTGAGGA CTGGACTTCG CGCGTCGGGA CGCCGCCTGG CCCAGTGCAG CGAAGTGATC 
ATCAAGACGA AGAAAACATT TCCCATGCAG ATAGATGGCG AGCCCTGGAT GCAGATGCCC 
TGCACGATCA AGGTGACCCA CAAGAACCAG GTGCCCATGC TGATGGCACC GCGGTCGGAA 
AAGGGACGCG GATTCTTCAA CCTGCTGTGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523654.2
CDS	444..2819
Translation	MRWELRYGWQIVTFRTNCATAAAVACHQLLCPVSLTSAVEIMPNSFPPEGRRRERRSGNLVLRAGCCCIGKYFLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_078930.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant G (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078930.4

ORF Insert Sequence:

ATGAGATGGG AACTTCGTTA TGGCTGGCAA ATTGTCACGT TCCGAACAAA TTGCGCTACT 
GCTGCCGCCG TTGCATGCCA CCAACTTTTG TGTCCTGTGT CTTTGACAAG TGCAGTGGAG 
ATAATGCCGA ATTCCTTTCC GCCAGAGGGT CGTCGGCGGG AGCGAAGGTC GGGAAACTTG 
GTCCTTCGAG CCGGATGCTG CTGCATCGGC AAATACTTTC TCCACAACCG CTGCGCCTCG 
TCGGTGAAGA AGGAGTGCAC CTTGGGCGAA TACTCCGAGC TAATTGTCCC GCCCACGGCC 
ATCTGCCCGG CGGTACTCGA CCGCCAGCGA TCCGTGAATC AGGCTCACAA GGCCACGCAC 
TTCCAAATCA CGCCGCCGGA CGAGCTGAGC TGCCCGCTGC TGGTGTTCGT CAATCCGAAG 
AGCGGCGGTC GCCAGGGGGA TCGCATCCTG CGGAAGTTCC AGTACATGCT CAATCCGCGC 
CAGGTGTACG ACCTGTCCAA GGGCGGGCCC AAGGAGGGAC TCACACTCTT CAAGGACCTG 
CCTCGCTTCA GGGTCATTTG CTGTGGCGGC GACGGCACCG TCGGCTGGGT TTTGGAAGCC 
ATGGACTCCA TAGAGTTGGC CAGCCAGCCG GCCATCGGAG TGATTCCCCT GGGAACGGGC 
AACGACCTGG CTCGCTGTCT CCGCTGGGGC GGTGGATACG AGGGCGAAAA CATCCCCAAA 
CTGATGGACA AGTTTCGAAG AGCCTCCACC GTCATGCTGG ACCGCTGGAG CATCGAGGTG 
ACCAATACTC CGCACAGCGA TGACATGCGA CCCAAGGTGA CGCTGCACTC GAACATGCAG 
AAGGTGATTG AGCTGTCGCA AAGTGTTGTG GTCGACAAAT CGCTGATGGA ACGCTTCGAG 
GAAATCCAAC GGCAGAGCAA GCAGGTGGCC ACGTCCATGG GCACGGCGGC CTCAAGCACA 
TCGATTATGA TGGCCAGCAA GACGGAAACG GAAATGGAAA CGATGGCCAC GATGGAGTTC 
GGCAGCAGCA CGACCACCAC CAACAGAACG ACCACGACCA AGAGCATTTC GATGTCCACG 
TTCGAGACGC AGTGCCTGCA ACAGACCCTG CGGACAGCGA TGAGCAGCAG CAGCAGCAAC 
ACGAGCAGCG GCAGTCCGTG CAACGGCAAC CAGGATGCGG AAACGGAAGT AGATTCCCAT 
GCCGCTGCCG CTGCCGATGT CCGGGAGAAG TCCGTGCCCA GGAGGTCGGG TGAAACGGAA 
AAGCAATCAC TTGAGACGCT GCTGCTGCAG CACAAACAGC AGATGCAACA ACAGCAGCAG 
CAACAGCAGC AAGGAGTCAC ATCACTAGCT GTCGAGGAAG CTGCAACAGC AACGCCAGTC 
GGGAGTAATC AAAGCGATAA TAGTAGCCAG CGCAATAAGC AAAACAACAT CCTTAAACAG 
CAAATTACAT TGTCATTGGA CTTGTCCGAC CATGAGGACG AGCCCAAGGA CGATGGCGGT 
GGCGCTGGCG ATGGTACCAA GAGCAACGGC AACAGCATTC CGGCAACACC AGCCACACCA 
ATCACACCCA CCACTCCGAA TGCCGCATCA AGTGTGCTGC AGCAACAACA GCAACAGCAT 
CTCCAGTTCG AGCAGCAACA GAAGCCCATC AAAGTGCAAT CCGACAAGGA CTGCACGGTG 
CCCTACAACA TAATCAACAA TTATTTCTCC GTCGGCGTGG ACGCCGCCAT CTGCGTCAAG 
TTCCACCTGG AGCGTGAGAA GAACCCGCAC AAATTCAACA GCCGCATGAA GAACAAGCTG 
TGGTACTTCG AGTATGCAAC ATCCGAGACA TTTGCGGCAT CCTGCAAGAA TCTCCACGAG 
AGCATCGAGA TTGTGTGCGA TGGCGTGGCC CTGGACTTGG CCAACGGACC CCACTTGCAG 
GGAGTGGCTC TGCTCAACAT CCCGTACACA CACGGCGGTT CCAACCTGTG GGGCGAGCAC 
CTGTCTCAGA AACGGATCCG CAAGAGTGCC GGCCCCTTCG GCAAGAGTAA GAAACTACGC 
GCCGGCGACA AGGAGTTCTC CGCCACCAGC TTCAATTCCG TGGACCTCTC GGTGGCCATT 
CAGGACTTTG GAGATCGGCT CATCGAGGTA ATTGGGCTGG AGAACTGCCT GCACATGGGC 
CAGGTGAGGA CTGGACTTCG CGCGTCGGGA CGCCGCCTGG CCCAGTGCAG CGAAGTGATC 
ATCAAGACGA AGAAAACATT TCCCATGCAG ATAGATGGCG AGCCCTGGAT GCAGATGCCC 
TGCACGATCA AGGTGACCCA CAAGAACCAG GTGCCCATGC TGATGGCACC GCGGTCGGAA 
AAGGGACGCG GATTCTTCAA CCTGCTGTGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_001103749.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097219.1 Drosophila melanogaster diacyl glycerol kinase, transcript variant E (Dgk), mRNA.

Price & Availability↑

CloneID	OFa09209
Clone ID Related Accession (Same CDS sequence)	NM_001103749.2
Accession Version	NM_001103749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_001103749.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GGCTCCATAC CAAACATCAA TAGCATTGCC GAGCTGATGC CGCAGTGCAG CGGAGGCGGA 
GGCGGCATCG GTGGCACGGG GGGCGTGGCC GGGGCCGAAG GGCACGCACA AGCCCGCAGC 
AGCTTTGTGG ACAAAATCCA TGGCATCACG GATAAATTAC ACCACTCGCT GGGCGGACAT 
CTATCCCACG ATCCTAGCAA GACCGGCAGT GTCCATCCGA TGCTCACGGT GACGCCGAGT 
CCCTTGGCCA GCGGACCCAG CATGTTCCAG GCGAGCAATC CGGCCCGTCG CTCCGTGGAC 
TCCAGTCCCT CGCACTCGGC CACCAACCAC TCGCAGATGT CTCGCAACTC CTCGAAGAAG 
AGCAGCAACT CGGTTAATTG CAAAATTGAT GCTGACATTA AGCTCCTCGC GCGAAAACTC 
TCCCATTTCG ATCCGCTGAC ACTCAAGGTC CCGCTGAAGG ATGTCGTCTG CTATTTATCA 
CTGCTCGAGG CAGGACGACC GGAGGACAAG CTGGAGTTCA TGTTCCGGCT GTACGACACG 
GATAGCAACG GAGTCCTGGA CACGGCCGAA ATGGACGCCA TTGTTAACCA GATGATGGCC 
GTGGCCGAAT ACCTCGGCTG GGATGTCAGC GAACTGCGTC CGATACTGCA AGAGATGATG 
GTTGAAATCG ACTACGATGC CGATGGCACG GTGTCCTTGG ACGAGTGGCA GCGAGGCGGG 
ATGACTACGA TTCCACTGCT GGTGCTCCTG GGTGTCGACA GCACCACGCT CAAGGAGGAT 
GGCATCCACG TGTGGCGCCT GAAGCACTTC AGCAAGCCGG CGTACTGCAA TCTCTGCCTC 
AACATGTTGG TGGGTCTCGG CAAGAAGGGC CTCTGCTGTG TGCTTTGCAA GTACACCGTC 
CATGAGCGAT GCGTGCAACA TGCTCCCGCG TCCTGCATCA CCACCTACGT CAAGTCCAAG 
AAACCAAAGT GCGGCGGAGA CCTACTGCAT CACTGGGTGG AGGGCAACTG CTATGGACGG 
TGCTCCAAGT GCCGCAAAAG GATAAAGGCC TACCACGGAA TCACAGGACT TACCTGCCGC 
TGGTGTCACA TGATGCTCCA CAACCGCTGC GCCTCGTCGG TGAAGAAGGA GTGCACCTTG 
GGCGAATACT CCGAGCTAAT TGTCCCGCCC ACGGCCATCT GCCCGGCGGT ACTCGACCGC 
CAGCGATCCG TGAATCAGGC TCACAAGAAA TCCCAAATGC ATCACCACCA GGCCACGCAC 
TTCCAAATCA CGCCGCCGGA CGAGCTGAGC TGCCCGCTGC TGGTGTTCGT CAATCCGAAG 
AGCGGCGGTC GCCAGGGGGA TCGCATCCTG CGGAAGTTCC AGTACATGCT CAATCCGCGC 
CAGGTGTACG ACCTGTCCAA GGGCGGGCCC AAGGAGGGAC TCACACTCTT CAAGGACCTG 
CCTCGCTTCA GGGTCATTTG CTGTGGCGGC GACGGCACCG TCGGCTGGGT TTTGGAAGCC 
ATGGACTCCA TAGAGTTGGC CAGCCAGCCG GCCATCGGAG TGATTCCCCT GGGAACGGGC 
AACGACCTGG CTCGCTGTCT CCGCTGGGGC GGTGGATACG AGGGCGAAAA CATCCCCAAA 
CTGATGGACA AGTTTCGAAG AGCCTCCACC GTCATGCTGG ACCGCTGGAG CATCGAGGTG 
ACCAATACTC CGCACAGCGA TGACATGCGA CCCAAGGTGA CGCTGCACTC GAACATGCAG 
AAGGTGATTG AGCTGTCGCA AAGTGTTGTG GTCGACAAAT CGCTGATGGA ACGCTTCGAG 
GAAATCCAAC GGCAGAGCAA GCAGGTGGCC ACGTCCATGG GCACGGCGGC CTCAAGCACA 
TCGATTATGA TGGCCAGCAA GACGGAAACG GAAATGGAAA CGATGGCCAC GATGGAGTTC 
GGCAGCAGCA CGACCACCAC CAACAGAACG ACCACGACCA AGAGCATTTC GATGTCCACG 
TTCGAGACGC AGTGCCTGCA ACAGACCCTG CGGACAGCGA TGAGCAGCAG CAGCAGCAAC 
ACGAGCAGCG GCAGTCCGTG CAACGGCAAC CAGGATGCGG AAACGGAAGT AGATTCCCAT 
GCCGCTGCCG CTGCCGATGT CCGGGAGAAG TCCGTGCCCA GGAGGTCGGG TGAAACGGAA 
AAGCAATCAC TTGAGACGCT GCTGCTGCAG CACAAACAGC AGATGCAACA ACAGCAGCAG 
CAACAGCAGC AAGGAGTCAC ATCACTAGCT GTCGAGGAAG CTGCAACAGC AACGCCAGTC 
GGGAGTAATC AAAGCGATAA TAGTAGCCAG CGCAATAAGC AAAACAACAT CCTTAAACAG 
CAAATTACAT TGTCATTGGA CTTGTCCGAC CATGAGGACG AGCCCAAGGA CGATGGCGGT 
GGCGCTGGCG ATGGTACCAA GAGCAACGGC AACAGCATTC CGGCAACACC AGCCACACCA 
ATCACACCCA CCACTCCGAA TGCCGCATCA AGTGTGCTGC AGCAACAACA GCAACAGCAT 
CTCCAGTTCG AGCAGCAACA GAAGCCCATC AAAGTGCAAT CCGACAAGGA CTGCACGGTG 
CCCTACAACA TAATCAACAA TTATTTCTCC GTCGGCGTGG ACGCCGCCAT CTGCGTCAAG 
TTCCACCTGG AGCGTGAGAA GAACCCGCAC AAATTCAACA GCCGCATGAA GAACAAGCTG 
TGGTACTTCG AGTATGCAAC ATCCGAGACA TTTGCGGCAT CCTGCAAGAA TCTCCACGAG 
AGCATCGAGA TTGTGTGCGA TGGCGTGGCC CTGGACTTGG CCAACGGACC CCACTTGCAG 
GGAGTGGCTC TGCTCAACAT CCCGTACACA CACGGCGGTT CCAACCTGTG GGGCGAGCAC 
CTGTCTCAGA AACGGATCCG CAAGAGTGCC GGCCCCTTCG GCAAGAGTAA GAAACTACGC 
GCCGGCGACA AGGAGTTCTC CGCCACCAGC TTCAATTCCG TGGACCTCTC GGTGGCCATT 
CAGGACTTTG GAGATCGGCT CATCGAGGTA ATTGGGCTGG AGAACTGCCT GCACATGGGC 
CAGGTGAGGA CTGGACTTCG CGCGTCGGGA CGCCGCCTGG CCCAGTGCAG CGAAGTGATC 
ATCAAGACGA AGAAAACATT TCCCATGCAG ATAGATGGCG AGCCCTGGAT GCAGATGCCC 
TGCACGATCA AGGTGACCCA CAAGAACCAG GTGCCCATGC TGATGGCACC GCGGTCGGAA 
AAGGGACGCG GATTCTTCAA CCTGCTGTGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097219.1
CDS	579..4214
Translation	MNIGIAAPKWDKLSPREFLQLQELASYSTRKLQDVLREFSSPSAASTPKCIPDGDIDFDGFRRFLDAFLDCEAPLDLAKHLFVSFLKPNVTQAQLHGRALNQMAAISSTAACAPVTSHTKGSIPNINSIAELMPQCSGGGGGIGGTGGVAGAEGHAQARSSFVDKIHGITDKLHHSLGGHLSHDPSKTGSVHPMLTVTPSPLASGPSMFQASNPARRSVDSSPSHSATNHSQMSRNSSKKSSNSVNCKIDADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKKSQMHHHQATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_001103749.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant E (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103749.2

ORF Insert Sequence:

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GGCTCCATAC CAAACATCAA TAGCATTGCC GAGCTGATGC CGCAGTGCAG CGGAGGCGGA 
GGCGGCATCG GTGGCACGGG GGGCGTGGCC GGGGCCGAAG GGCACGCACA AGCCCGCAGC 
AGCTTTGTGG ACAAAATCCA TGGCATCACG GATAAATTAC ACCACTCGCT GGGCGGACAT 
CTATCCCACG ATCCTAGCAA GACCGGCAGT GTCCATCCGA TGCTCACGGT GACGCCGAGT 
CCCTTGGCCA GCGGACCCAG CATGTTCCAG GCGAGCAATC CGGCCCGTCG CTCCGTGGAC 
TCCAGTCCCT CGCACTCGGC CACCAACCAC TCGCAGATGT CTCGCAACTC CTCGAAGAAG 
AGCAGCAACT CGGTTAATTG CAAAATTGAT GCTGACATTA AGCTCCTCGC GCGAAAACTC 
TCCCATTTCG ATCCGCTGAC ACTCAAGGTC CCGCTGAAGG ATGTCGTCTG CTATTTATCA 
CTGCTCGAGG CAGGACGACC GGAGGACAAG CTGGAGTTCA TGTTCCGGCT GTACGACACG 
GATAGCAACG GAGTCCTGGA CACGGCCGAA ATGGACGCCA TTGTTAACCA GATGATGGCC 
GTGGCCGAAT ACCTCGGCTG GGATGTCAGC GAACTGCGTC CGATACTGCA AGAGATGATG 
GTTGAAATCG ACTACGATGC CGATGGCACG GTGTCCTTGG ACGAGTGGCA GCGAGGCGGG 
ATGACTACGA TTCCACTGCT GGTGCTCCTG GGTGTCGACA GCACCACGCT CAAGGAGGAT 
GGCATCCACG TGTGGCGCCT GAAGCACTTC AGCAAGCCGG CGTACTGCAA TCTCTGCCTC 
AACATGTTGG TGGGTCTCGG CAAGAAGGGC CTCTGCTGTG TGCTTTGCAA GTACACCGTC 
CATGAGCGAT GCGTGCAACA TGCTCCCGCG TCCTGCATCA CCACCTACGT CAAGTCCAAG 
AAACCAAAGT GCGGCGGAGA CCTACTGCAT CACTGGGTGG AGGGCAACTG CTATGGACGG 
TGCTCCAAGT GCCGCAAAAG GATAAAGGCC TACCACGGAA TCACAGGACT TACCTGCCGC 
TGGTGTCACA TGATGCTCCA CAACCGCTGC GCCTCGTCGG TGAAGAAGGA GTGCACCTTG 
GGCGAATACT CCGAGCTAAT TGTCCCGCCC ACGGCCATCT GCCCGGCGGT ACTCGACCGC 
CAGCGATCCG TGAATCAGGC TCACAAGAAA TCCCAAATGC ATCACCACCA GGCCACGCAC 
TTCCAAATCA CGCCGCCGGA CGAGCTGAGC TGCCCGCTGC TGGTGTTCGT CAATCCGAAG 
AGCGGCGGTC GCCAGGGGGA TCGCATCCTG CGGAAGTTCC AGTACATGCT CAATCCGCGC 
CAGGTGTACG ACCTGTCCAA GGGCGGGCCC AAGGAGGGAC TCACACTCTT CAAGGACCTG 
CCTCGCTTCA GGGTCATTTG CTGTGGCGGC GACGGCACCG TCGGCTGGGT TTTGGAAGCC 
ATGGACTCCA TAGAGTTGGC CAGCCAGCCG GCCATCGGAG TGATTCCCCT GGGAACGGGC 
AACGACCTGG CTCGCTGTCT CCGCTGGGGC GGTGGATACG AGGGCGAAAA CATCCCCAAA 
CTGATGGACA AGTTTCGAAG AGCCTCCACC GTCATGCTGG ACCGCTGGAG CATCGAGGTG 
ACCAATACTC CGCACAGCGA TGACATGCGA CCCAAGGTGA CGCTGCACTC GAACATGCAG 
AAGGTGATTG AGCTGTCGCA AAGTGTTGTG GTCGACAAAT CGCTGATGGA ACGCTTCGAG 
GAAATCCAAC GGCAGAGCAA GCAGGTGGCC ACGTCCATGG GCACGGCGGC CTCAAGCACA 
TCGATTATGA TGGCCAGCAA GACGGAAACG GAAATGGAAA CGATGGCCAC GATGGAGTTC 
GGCAGCAGCA CGACCACCAC CAACAGAACG ACCACGACCA AGAGCATTTC GATGTCCACG 
TTCGAGACGC AGTGCCTGCA ACAGACCCTG CGGACAGCGA TGAGCAGCAG CAGCAGCAAC 
ACGAGCAGCG GCAGTCCGTG CAACGGCAAC CAGGATGCGG AAACGGAAGT AGATTCCCAT 
GCCGCTGCCG CTGCCGATGT CCGGGAGAAG TCCGTGCCCA GGAGGTCGGG TGAAACGGAA 
AAGCAATCAC TTGAGACGCT GCTGCTGCAG CACAAACAGC AGATGCAACA ACAGCAGCAG 
CAACAGCAGC AAGGAGTCAC ATCACTAGCT GTCGAGGAAG CTGCAACAGC AACGCCAGTC 
GGGAGTAATC AAAGCGATAA TAGTAGCCAG CGCAATAAGC AAAACAACAT CCTTAAACAG 
CAAATTACAT TGTCATTGGA CTTGTCCGAC CATGAGGACG AGCCCAAGGA CGATGGCGGT 
GGCGCTGGCG ATGGTACCAA GAGCAACGGC AACAGCATTC CGGCAACACC AGCCACACCA 
ATCACACCCA CCACTCCGAA TGCCGCATCA AGTGTGCTGC AGCAACAACA GCAACAGCAT 
CTCCAGTTCG AGCAGCAACA GAAGCCCATC AAAGTGCAAT CCGACAAGGA CTGCACGGTG 
CCCTACAACA TAATCAACAA TTATTTCTCC GTCGGCGTGG ACGCCGCCAT CTGCGTCAAG 
TTCCACCTGG AGCGTGAGAA GAACCCGCAC AAATTCAACA GCCGCATGAA GAACAAGCTG 
TGGTACTTCG AGTATGCAAC ATCCGAGACA TTTGCGGCAT CCTGCAAGAA TCTCCACGAG 
AGCATCGAGA TTGTGTGCGA TGGCGTGGCC CTGGACTTGG CCAACGGACC CCACTTGCAG 
GGAGTGGCTC TGCTCAACAT CCCGTACACA CACGGCGGTT CCAACCTGTG GGGCGAGCAC 
CTGTCTCAGA AACGGATCCG CAAGAGTGCC GGCCCCTTCG GCAAGAGTAA GAAACTACGC 
GCCGGCGACA AGGAGTTCTC CGCCACCAGC TTCAATTCCG TGGACCTCTC GGTGGCCATT 
CAGGACTTTG GAGATCGGCT CATCGAGGTA ATTGGGCTGG AGAACTGCCT GCACATGGGC 
CAGGTGAGGA CTGGACTTCG CGCGTCGGGA CGCCGCCTGG CCCAGTGCAG CGAAGTGATC 
ATCAAGACGA AGAAAACATT TCCCATGCAG ATAGATGGCG AGCCCTGGAT GCAGATGCCC 
TGCACGATCA AGGTGACCCA CAAGAACCAG GTGCCCATGC TGATGGCACC GCGGTCGGAA 
AAGGGACGCG GATTCTTCAA CCTGCTGTGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_206053.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995775.2 Drosophila melanogaster diacyl glycerol kinase, transcript variant I (Dgk), mRNA.

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CloneID	OFa09210
Clone ID Related Accession (Same CDS sequence)	NM_206053.2
Accession Version	NM_206053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_206053.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATGGG AACTTCGTTA TGGCTGGCAA ATTGTCACGT TCCGAACAAA TTGCGCTACT 
GCTGCCGCCG TTGCATGCCA CCAACTTTTG TGTCCTGTGT CTTTGACAAG TGCAGTGGAG 
ATAATGCCGA ATTCCTTTCC GCCAGAGGGT CGTCGGCGGG AGCGAAGGTC GGGAAACTTG 
GTCCTTCGAG CCGGATGCTG CTGCATCGGC AAATACTTTG CCACGCACTT CCAAATCACG 
CCGCCGGACG AGCTGAGCTG CCCGCTGCTG GTGTTCGTCA ATCCGAAGAG CGGCGGTCGC 
CAGGGGGATC GCATCCTGCG GAAGTTCCAG TACATGCTCA ATCCGCGCCA GGTGTACGAC 
CTGTCCAAGG GCGGGCCCAA GGAGGGACTC ACACTCTTCA AGGACCTGCC TCGCTTCAGG 
GTCATTTGCT GTGGCGGCGA CGGCACCGTC GGCTGGGTTT TGGAAGCCAT GGACTCCATA 
GAGTTGGCCA GCCAGCCGGC CATCGGAGTG ATTCCCCTGG GAACGGGCAA CGACCTGGCT 
CGCTGTCTCC GCTGGGGCGG TGGATACGAG GGCGAAAACA TCCCCAAACT GATGGACAAG 
TTTCGAAGAG CCTCCACCGT CATGCTGGAC CGCTGGAGCA TCGAGGTGAC CAATACTCCG 
CACAGCGATG ACATGCGACC CAAGGTGACG CTGCACTCGA ACATGCAGAA GGTGATTGAG 
CTGTCGCAAA GTGTTGTGGT CGACAAATCG CTGATGGAAC GCTTCGAGGA AATCCAACGG 
CAGAGCAAGC AGGTGGCCAC GTCCATGGGC ACGGCGGCCT CAAGCACATC GATTATGATG 
GCCAGCAAGA CGGAAACGGA AATGGAAACG ATGGCCACGA TGGAGTTCGG CAGCAGCACG 
ACCACCACCA ACAGAACGAC CACGACCAAG AGCATTTCGA TGTCCACGTT CGAGACGCAG 
TGCCTGCAAC AGACCCTGCG GACAGCGATG AGCAGCAGCA GCAGCAACAC GAGCAGCGGC 
AGTCCGTGCA ACGGCAACCA GGATGCGGAA ACGGAAGTAG ATTCCCATGC CGCTGCCGCT 
GCCGATGTCC GGGAGAAGTC CGTGCCCAGG AGGTCGGGTG AAACGGAAAA GCAATCACTT 
GAGACGCTGC TGCTGCAGCA CAAACAGCAG ATGCAACAAC AGCAGCAGCA ACAGCAGCAA 
GGAGTCACAT CACTAGCTGT CGAGGAAGCT GCAACAGCAA CGCCAGTCGG GAGTAATCAA 
AGCGATAATA GTAGCCAGCG CAATAAGCAA AACAACATCC TTAAACAGCA AATTACATTG 
TCATTGGACT TGTCCGACCA TGAGGACGAG CCCAAGGACG ATGGCGGTGG CGCTGGCGAT 
GGTACCAAGA GCAACGGCAA CAGCATTCCG GCAACACCAG CCACACCAAT CACACCCACC 
ACTCCGAATG CCGCATCAAG TGTGCTGCAG CAACAACAGC AACAGCATCT CCAGTTCGAG 
CAGCAACAGA AGCCCATCAA AGTGCAATCC GACAAGGACT GCACGGTGCC CTACAACATA 
ATCAACAATT ATTTCTCCGT CGGCGTGGAC GCCGCCATCT GCGTCAAGTT CCACCTGGAG 
CGTGAGAAGA ACCCGCACAA ATTCAACAGC CGCATGAAGA ACAAGCTGTG GTACTTCGAG 
TATGCAACAT CCGAGACATT TGCGGCATCC TGCAAGAATC TCCACGAGAG CATCGAGATT 
GTGTGCGATG GCGTGGCCCT GGACTTGGCC AACGGACCCC ACTTGCAGGG AGTGGCTCTG 
CTCAACATCC CGTACACACA CGGCGGTTCC AACCTGTGGG GCGAGCACCT GTCTCAGAAA 
CGGATCCGCA AGAGTGCCGG CCCCTTCGGC AAGAGTAAGA AACTACGCGC CGGCGACAAG 
GAGTTCTCCG CCACCAGCTT CAATTCCGTG GACCTCTCGG TGGCCATTCA GGACTTTGGA 
GATCGGCTCA TCGAGGTAAT TGGGCTGGAG AACTGCCTGC ACATGGGCCA GGTGAGGACT 
GGACTTCGCG CGTCGGGACG CCGCCTGGCC CAGTGCAGCG AAGTGATCAT CAAGACGAAG 
AAAACATTTC CCATGCAGAT AGATGGCGAG CCCTGGATGC AGATGCCCTG CACGATCAAG 
GTGACCCACA AGAACCAGGT GCCCATGCTG ATGGCACCGC GGTCGGAAAA GGGACGCGGA 
TTCTTCAACC TGCTGTGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995775.2
CDS	444..2687
Translation	MRWELRYGWQIVTFRTNCATAAAVACHQLLCPVSLTSAVEIMPNSFPPEGRRRERRSGNLVLRAGCCCIGKYFATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_206053.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant I (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206053.2

ORF Insert Sequence:

ATGAGATGGG AACTTCGTTA TGGCTGGCAA ATTGTCACGT TCCGAACAAA TTGCGCTACT 
GCTGCCGCCG TTGCATGCCA CCAACTTTTG TGTCCTGTGT CTTTGACAAG TGCAGTGGAG 
ATAATGCCGA ATTCCTTTCC GCCAGAGGGT CGTCGGCGGG AGCGAAGGTC GGGAAACTTG 
GTCCTTCGAG CCGGATGCTG CTGCATCGGC AAATACTTTG CCACGCACTT CCAAATCACG 
CCGCCGGACG AGCTGAGCTG CCCGCTGCTG GTGTTCGTCA ATCCGAAGAG CGGCGGTCGC 
CAGGGGGATC GCATCCTGCG GAAGTTCCAG TACATGCTCA ATCCGCGCCA GGTGTACGAC 
CTGTCCAAGG GCGGGCCCAA GGAGGGACTC ACACTCTTCA AGGACCTGCC TCGCTTCAGG 
GTCATTTGCT GTGGCGGCGA CGGCACCGTC GGCTGGGTTT TGGAAGCCAT GGACTCCATA 
GAGTTGGCCA GCCAGCCGGC CATCGGAGTG ATTCCCCTGG GAACGGGCAA CGACCTGGCT 
CGCTGTCTCC GCTGGGGCGG TGGATACGAG GGCGAAAACA TCCCCAAACT GATGGACAAG 
TTTCGAAGAG CCTCCACCGT CATGCTGGAC CGCTGGAGCA TCGAGGTGAC CAATACTCCG 
CACAGCGATG ACATGCGACC CAAGGTGACG CTGCACTCGA ACATGCAGAA GGTGATTGAG 
CTGTCGCAAA GTGTTGTGGT CGACAAATCG CTGATGGAAC GCTTCGAGGA AATCCAACGG 
CAGAGCAAGC AGGTGGCCAC GTCCATGGGC ACGGCGGCCT CAAGCACATC GATTATGATG 
GCCAGCAAGA CGGAAACGGA AATGGAAACG ATGGCCACGA TGGAGTTCGG CAGCAGCACG 
ACCACCACCA ACAGAACGAC CACGACCAAG AGCATTTCGA TGTCCACGTT CGAGACGCAG 
TGCCTGCAAC AGACCCTGCG GACAGCGATG AGCAGCAGCA GCAGCAACAC GAGCAGCGGC 
AGTCCGTGCA ACGGCAACCA GGATGCGGAA ACGGAAGTAG ATTCCCATGC CGCTGCCGCT 
GCCGATGTCC GGGAGAAGTC CGTGCCCAGG AGGTCGGGTG AAACGGAAAA GCAATCACTT 
GAGACGCTGC TGCTGCAGCA CAAACAGCAG ATGCAACAAC AGCAGCAGCA ACAGCAGCAA 
GGAGTCACAT CACTAGCTGT CGAGGAAGCT GCAACAGCAA CGCCAGTCGG GAGTAATCAA 
AGCGATAATA GTAGCCAGCG CAATAAGCAA AACAACATCC TTAAACAGCA AATTACATTG 
TCATTGGACT TGTCCGACCA TGAGGACGAG CCCAAGGACG ATGGCGGTGG CGCTGGCGAT 
GGTACCAAGA GCAACGGCAA CAGCATTCCG GCAACACCAG CCACACCAAT CACACCCACC 
ACTCCGAATG CCGCATCAAG TGTGCTGCAG CAACAACAGC AACAGCATCT CCAGTTCGAG 
CAGCAACAGA AGCCCATCAA AGTGCAATCC GACAAGGACT GCACGGTGCC CTACAACATA 
ATCAACAATT ATTTCTCCGT CGGCGTGGAC GCCGCCATCT GCGTCAAGTT CCACCTGGAG 
CGTGAGAAGA ACCCGCACAA ATTCAACAGC CGCATGAAGA ACAAGCTGTG GTACTTCGAG 
TATGCAACAT CCGAGACATT TGCGGCATCC TGCAAGAATC TCCACGAGAG CATCGAGATT 
GTGTGCGATG GCGTGGCCCT GGACTTGGCC AACGGACCCC ACTTGCAGGG AGTGGCTCTG 
CTCAACATCC CGTACACACA CGGCGGTTCC AACCTGTGGG GCGAGCACCT GTCTCAGAAA 
CGGATCCGCA AGAGTGCCGG CCCCTTCGGC AAGAGTAAGA AACTACGCGC CGGCGACAAG 
GAGTTCTCCG CCACCAGCTT CAATTCCGTG GACCTCTCGG TGGCCATTCA GGACTTTGGA 
GATCGGCTCA TCGAGGTAAT TGGGCTGGAG AACTGCCTGC ACATGGGCCA GGTGAGGACT 
GGACTTCGCG CGTCGGGACG CCGCCTGGCC CAGTGCAGCG AAGTGATCAT CAAGACGAAG 
AAAACATTTC CCATGCAGAT AGATGGCGAG CCCTGGATGC AGATGCCCTG CACGATCAAG 
GTGACCCACA AGAACCAGGT GCCCATGCTG ATGGCACCGC GGTCGGAAAA GGGACGCGGA 
TTCTTCAACC TGCTGTGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_001273863.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260792.1 Drosophila melanogaster diacyl glycerol kinase, transcript variant J (Dgk), mRNA.

Price & Availability↑

CloneID	OFa12763
Clone ID Related Accession (Same CDS sequence)	NM_001273863.1
Accession Version	NM_001273863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGGC CACGCACTTC CAAATCACGC CGCCGGACGA GCTGAGCTGC 
CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC GGCGGTCGCC AGGGGGATCG CATCCTGCGG 
AAGTTCCAGT ACATGCTCAA TCCGCGCCAG GTGTACGACC TGTCCAAGGG CGGGCCCAAG 
GAGGGACTCA CACTCTTCAA GGACCTGCCT CGCTTCAGGG TCATTTGCTG TGGCGGCGAC 
GGCACCGTCG GCTGGGTTTT GGAAGCCATG GACTCCATAG AGTTGGCCAG CCAGCCGGCC 
ATCGGAGTGA TTCCCCTGGG AACGGGCAAC GACCTGGCTC GCTGTCTCCG CTGGGGCGGT 
GGATACGAGG GCGAAAACAT CCCCAAACTG ATGGACAAGT TTCGAAGAGC CTCCACCGTC 
ATGCTGGACC GCTGGAGCAT CGAGGTGACC AATACTCCGC ACAGCGATGA CATGCGACCC 
AAGGTGACGC TGCACTCGAA CATGCAGAAG GTGATTGAGC TGTCGCAAAG TGTTGTGGTC 
GACAAATCGC TGATGGAACG CTTCGAGGAA ATCCAACGGC AGAGCAAGCA GGTGGCCACG 
TCCATGGGCA CGGCGGCCTC AAGCACATCG ATTATGATGG CCAGCAAGAC GGAAACGGAA 
ATGGAAACGA TGGCCACGAT GGAGTTCGGC AGCAGCACGA CCACCACCAA CAGAACGACC 
ACGACCAAGA GCATTTCGAT GTCCACGTTC GAGACGCAGT GCCTGCAACA GACCCTGCGG 
ACAGCGATGA GCAGCAGCAG CAGCAACACG AGCAGCGGCA GTCCGTGCAA CGGCAACCAG 
GATGCGGAAA CGGAAGTAGA TTCCCATGCC GCTGCCGCTG CCGATGTCCG GGAGAAGTCC 
GTGCCCAGGA GGTCGGGTGA AACGGAAAAG CAATCACTTG AGACGCTGCT GCTGCAGCAC 
AAACAGCAGA TGCAACAACA GCAGCAGCAA CAGCAGCAAG GAGTCACATC ACTAGCTGTC 
GAGGAAGCTG CAACAGCAAC GCCAGTCGGG AGTAATCAAA GCGATAATAG TAGCCAGCGC 
AATAAGCAAA ACAACATCCT TAAACAGCAA ATTACATTGT CATTGGACTT GTCCGACCAT 
GAGGACGAGC CCAAGGACGA TGGCGGTGGC GCTGGCGATG GTACCAAGAG CAACGGCAAC 
AGCATTCCGG CAACACCAGC CACACCAATC ACACCCACCA CTCCGAATGC CGCATCAAGT 
GTGCTGCAGC AACAACAGCA ACAGCATCTC CAGTTCGAGC AGCAACAGAA GCCCATCAAA 
GTGCAATCCG ACAAGGACTG CACGGTGCCC TACAACATAA TCAACAATTA TTTCTCCGTC 
GGCGTGGACG CCGCCATCTG CGTCAAGTTC CACCTGGAGC GTGAGAAGAA CCCGCACAAA 
TTCAACAGCC GCATGAAGAA CAAGCTGTGG TACTTCGAGT ATGCAACATC CGAGACATTT 
GCGGCATCCT GCAAGAATCT CCACGAGAGC ATCGAGATTG TGTGCGATGG CGTGGCCCTG 
GACTTGGCCA ACGGACCCCA CTTGCAGGGA GTGGCTCTGC TCAACATCCC GTACACACAC 
GGCGGTTCCA ACCTGTGGGG CGAGCACCTG TCTCAGAAAC GGATCCGCAA GAGTGCCGGC 
CCCTTCGGCA AGAGTAAGAA ACTACGCGCC GGCGACAAGG AGTTCTCCGC CACCAGCTTC 
AATTCCGTGG ACCTCTCGGT GGCCATTCAG GACTTTGGAG ATCGGCTCAT CGAGGTAATT 
GGGCTGGAGA ACTGCCTGCA CATGGGCCAG GTGAGGACTG GACTTCGCGC GTCGGGACGC 
CGCCTGGCCC AGTGCAGCGA AGTGATCATC AAGACGAAGA AAACATTTCC CATGCAGATA 
GATGGCGAGC CCTGGATGCA GATGCCCTGC ACGATCAAGG TGACCCACAA GAACCAGGTG 
CCCATGCTGA TGGCACCGCG GTCGGAAAAG GGACGCGGAT TCTTCAACCT GCTGTGCAGC 
TGATTCAGGC GCAGCGCATC GAGCGACTTC ATGGGCCGGA GTGCCCAGGA GTCGGCCGAT 
GAGCTGGATG ATGACTTTCG GATCGTCCCT AGAAGCACCA CCGTCCACAA CATAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260792.1
CDS	579..3998
Translation	MNIGIAAPKWDKLSPREFLQLQELASYSTRKLQDVLREFSSPSAASTPKCIPDGDIDFDGFRRFLDAFLDCEAPLDLAKHLFVSFLKPNVTQAQLHGRALNQMAAISSTAACAPVTSHTKAVSRILPAIGRHCPGSLESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS*FRRSASSDFMGRSAQESADELDDDFRIVPRSTTVHNIA

Target ORF information:

RefSeq Version	NM_001273863.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant J (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273863.1

ORF Insert Sequence:

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGGC CACGCACTTC CAAATCACGC CGCCGGACGA GCTGAGCTGC 
CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC GGCGGTCGCC AGGGGGATCG CATCCTGCGG 
AAGTTCCAGT ACATGCTCAA TCCGCGCCAG GTGTACGACC TGTCCAAGGG CGGGCCCAAG 
GAGGGACTCA CACTCTTCAA GGACCTGCCT CGCTTCAGGG TCATTTGCTG TGGCGGCGAC 
GGCACCGTCG GCTGGGTTTT GGAAGCCATG GACTCCATAG AGTTGGCCAG CCAGCCGGCC 
ATCGGAGTGA TTCCCCTGGG AACGGGCAAC GACCTGGCTC GCTGTCTCCG CTGGGGCGGT 
GGATACGAGG GCGAAAACAT CCCCAAACTG ATGGACAAGT TTCGAAGAGC CTCCACCGTC 
ATGCTGGACC GCTGGAGCAT CGAGGTGACC AATACTCCGC ACAGCGATGA CATGCGACCC 
AAGGTGACGC TGCACTCGAA CATGCAGAAG GTGATTGAGC TGTCGCAAAG TGTTGTGGTC 
GACAAATCGC TGATGGAACG CTTCGAGGAA ATCCAACGGC AGAGCAAGCA GGTGGCCACG 
TCCATGGGCA CGGCGGCCTC AAGCACATCG ATTATGATGG CCAGCAAGAC GGAAACGGAA 
ATGGAAACGA TGGCCACGAT GGAGTTCGGC AGCAGCACGA CCACCACCAA CAGAACGACC 
ACGACCAAGA GCATTTCGAT GTCCACGTTC GAGACGCAGT GCCTGCAACA GACCCTGCGG 
ACAGCGATGA GCAGCAGCAG CAGCAACACG AGCAGCGGCA GTCCGTGCAA CGGCAACCAG 
GATGCGGAAA CGGAAGTAGA TTCCCATGCC GCTGCCGCTG CCGATGTCCG GGAGAAGTCC 
GTGCCCAGGA GGTCGGGTGA AACGGAAAAG CAATCACTTG AGACGCTGCT GCTGCAGCAC 
AAACAGCAGA TGCAACAACA GCAGCAGCAA CAGCAGCAAG GAGTCACATC ACTAGCTGTC 
GAGGAAGCTG CAACAGCAAC GCCAGTCGGG AGTAATCAAA GCGATAATAG TAGCCAGCGC 
AATAAGCAAA ACAACATCCT TAAACAGCAA ATTACATTGT CATTGGACTT GTCCGACCAT 
GAGGACGAGC CCAAGGACGA TGGCGGTGGC GCTGGCGATG GTACCAAGAG CAACGGCAAC 
AGCATTCCGG CAACACCAGC CACACCAATC ACACCCACCA CTCCGAATGC CGCATCAAGT 
GTGCTGCAGC AACAACAGCA ACAGCATCTC CAGTTCGAGC AGCAACAGAA GCCCATCAAA 
GTGCAATCCG ACAAGGACTG CACGGTGCCC TACAACATAA TCAACAATTA TTTCTCCGTC 
GGCGTGGACG CCGCCATCTG CGTCAAGTTC CACCTGGAGC GTGAGAAGAA CCCGCACAAA 
TTCAACAGCC GCATGAAGAA CAAGCTGTGG TACTTCGAGT ATGCAACATC CGAGACATTT 
GCGGCATCCT GCAAGAATCT CCACGAGAGC ATCGAGATTG TGTGCGATGG CGTGGCCCTG 
GACTTGGCCA ACGGACCCCA CTTGCAGGGA GTGGCTCTGC TCAACATCCC GTACACACAC 
GGCGGTTCCA ACCTGTGGGG CGAGCACCTG TCTCAGAAAC GGATCCGCAA GAGTGCCGGC 
CCCTTCGGCA AGAGTAAGAA ACTACGCGCC GGCGACAAGG AGTTCTCCGC CACCAGCTTC 
AATTCCGTGG ACCTCTCGGT GGCCATTCAG GACTTTGGAG ATCGGCTCAT CGAGGTAATT 
GGGCTGGAGA ACTGCCTGCA CATGGGCCAG GTGAGGACTG GACTTCGCGC GTCGGGACGC 
CGCCTGGCCC AGTGCAGCGA AGTGATCATC AAGACGAAGA AAACATTTCC CATGCAGATA 
GATGGCGAGC CCTGGATGCA GATGCCCTGC ACGATCAAGG TGACCCACAA GAACCAGGTG 
CCCATGCTGA TGGCACCGCG GTCGGAAAAG GGACGCGGAT TCTTCAACCT GCTGTGCAGC 
TGATTCAGGC GCAGCGCATC GAGCGACTTC ATGGGCCGGA GTGCCCAGGA GTCGGCCGAT 
GAGCTGGATG ATGACTTTCG GATCGTCCCT AGAAGCACCA CCGTCCACAA CATAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgk ( NM_001316408.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303337.1 Drosophila melanogaster diacyl glycerol kinase, transcript variant K (Dgk), mRNA.

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CloneID	OFa41313
Clone ID Related Accession (Same CDS sequence)	NM_001316408.1
Accession Version	NM_001316408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diacyl glycerol kinase, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGGC CACGCACTTC CAAATCACGC CGCCGGACGA GCTGAGCTGC 
CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC GGCGGTCGCC AGGGGGATCG CATCCTGCGG 
AAGTTCCAGT ACATGCTCAA TCCGCGCCAG GTGTACGACC TGTCCAAGGG CGGGCCCAAG 
GAGGGACTCA CACTCTTCAA GGACCTGCCT CGCTTCAGGG TCATTTGCTG TGGCGGCGAC 
GGCACCGTCG GCTGGGTTTT GGAAGCCATG GACTCCATAG AGTTGGCCAG CCAGCCGGCC 
ATCGGAGTGA TTCCCCTGGG AACGGGCAAC GACCTGGCTC GCTGTCTCCG CTGGGGCGGT 
GGATACGAGG GCGAAAACAT CCCCAAACTG ATGGACAAGT TTCGAAGAGC CTCCACCGTC 
ATGCTGGACC GCTGGAGCAT CGAGGTGACC AATACTCCGC ACAGCGATGA CATGCGACCC 
AAGGTGACGC TGCACTCGAA CATGCAGAAG GTGATTGAGC TGTCGCAAAG TGTTGTGGTC 
GACAAATCGC TGATGGAACG CTTCGAGGAA ATCCAACGGC AGAGCAAGCA GGTGGCCACG 
TCCATGGGCA CGGCGGCCTC AAGCACATCG ATTATGATGG CCAGCAAGAC GGAAACGGAA 
ATGGAAACGA TGGCCACGAT GGAGTTCGGC AGCAGCACGA CCACCACCAA CAGAACGACC 
ACGACCAAGA GCATTTCGAT GTCCACGTTC GAGACGCAGT GCCTGCAACA GACCCTGCGG 
ACAGCGATGA GCAGCAGCAG CAGCAACACG AGCAGCGGCA GTCCGTGCAA CGGCAACCAG 
GATGCGGAAA CGGAAGTAGA TTCCCATGCC GCTGCCGCTG CCGATGTCCG GGAGAAGTCC 
GTGCCCAGGA GGTCGGGTGA AACGGAAAAG CAATCACTTG AGACGCTGCT GCTGCAGCAC 
AAACAGCAGA TGCAACAACA GCAGCAGCAA CAGCAGCAAG GAGTCACATC ACTAGCTGTC 
GAGGAAGCTG CAACAGCAAC GCCAGTCGGG AGTAATCAAA GCGATAATAG TAGCCAGCGC 
AATAAGCAAA ACAACATCCT TAAACAGCAA ATTACATTGT CATTGGACTT GTCCGACCAT 
GAGGACGAGC CCAAGGACGA TGGCGGTGGC GCTGGCGATG GTACCAAGAG CAACGGCAAC 
AGCATTCCGG CAACACCAGC CACACCAATC ACACCCACCA CTCCGAATGC CGCATCAAGT 
GTGCTGCAGC AACAACAGCA ACAGCATCTC CAGTTCGAGC AGCAACAGAA GCCCATCAAA 
GTGCAATCCG ACAAGGACTG CACGGTGCCC TACAACATAA TCAACAATTA TTTCTCCGTC 
GGCGTGGACG CCGCCATCTG CGTCAAGTTC CACCTGGAGC GTGAGAAGAA CCCGCACAAA 
TTCAACAGCC GCATGAAGAA CAAGCTGTGG TACTTCGAGT ATGCAACATC CGAGACATTT 
GCGGCATCCT GCAAGAATCT CCACGAGAGC ATCGAGATTG TGTGCGATGG CGTGGCCCTG 
GACTTGGCCA ACGGACCCCA CTTGCAGGGA GTGGCTCTGC TCAACATCCC GTACACACAC 
GGCGGTTCCA ACCTGTGGGG CGAGCACCTG TCTCAGAAAC GGATCCGCAA GAGTGCCGGC 
CCCTTCGGCA AGAGTAAGAA ACTACGCGCC GGCGACAAGG AGTTCTCCGC CACCAGCTTC 
AATTCCGTGG ACCTCTCGGT GGCCATTCAG GACTTTGGAG ATCGGCTCAT CGAGGTAATT 
GGGCTGGAGA ACTGCCTGCA CATGGGCCAG GTGAGGACTG GACTTCGCGC GTCGGGACGC 
CGCCTGGCCC AGTGCAGCGA AGTGATCATC AAGACGAAGA AAACATTTCC CATGCAGATA 
GATGGCGAGC CCTGGATGCA GATGCCCTGC ACGATCAAGG TGACCCACAA GAACCAGGTG 
CCCATGCTGA TGGCACCGCG GTCGGAAAAG GGACGCGGAT TCTTCAACCT GCTGTGCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303337.1
CDS	579..3881
Translation	MNIGIAAPKWDKLSPREFLQLQELASYSTRKLQDVLREFSSPSAASTPKCIPDGDIDFDGFRRFLDAFLDCEAPLDLAKHLFVSFLKPNVTQAQLHGRALNQMAAISSTAACAPVTSHTKAVSRILPAIGRHCPGSLESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRSVNQAHKATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIELASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSIEVTNTPHSDDMRPKVTLHSNMQKVIELSQSVVVDKSLMERFEEIQRQSKQVATSMGTAASSTSIMMASKTETEMETMATMEFGSSTTTTNRTTTTKSISMSTFETQCLQQTLRTAMSSSSSNTSSGSPCNGNQDAETEVDSHAAAAADVREKSVPRRSGETEKQSLETLLLQHKQQMQQQQQQQQQGVTSLAVEEAATATPVGSNQSDNSSQRNKQNNILKQQITLSLDLSDHEDEPKDDGGGAGDGTKSNGNSIPATPATPITPTTPNAASSVLQQQQQQHLQFEQQQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSVDLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAPRSEKGRGFFNLLCS

Target ORF information:

RefSeq Version	NM_001316408.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diacyl glycerol kinase, transcript variant K (Dgk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316408.1

ORF Insert Sequence:

ATGAATATTG GCATCGCAGC ACCGAAATGG GATAAATTGT CGCCACGCGA GTTTTTGCAG 
CTGCAGGAGT TGGCCTCATA TTCTACCCGA AAGCTGCAGG ATGTGCTGCG GGAGTTCAGC 
TCGCCGAGTG CCGCCAGCAC GCCCAAGTGC ATTCCGGATG GGGACATTGA TTTCGACGGG 
TTCCGGCGCT TCCTGGACGC CTTCCTCGAC TGCGAGGCGC CGTTGGACCT GGCCAAACAT 
TTGTTTGTGT CCTTCCTCAA GCCGAATGTC ACCCAGGCCC AGCTGCATGG CCGGGCACTT 
AATCAAATGG CCGCCATCAG CAGCACGGCC GCCTGTGCGC CGGTCACGTC GCACACGAAG 
GCAGTTTCAC GCATTTTGCC AGCAATTGGA CGGCATTGTC CTGGAAGTTT AGAGAGTGGG 
AGTGCACAGG CAGAAAAGAG GGCTGACATT AAGCTCCTCG CGCGAAAACT CTCCCATTTC 
GATCCGCTGA CACTCAAGGT CCCGCTGAAG GATGTCGTCT GCTATTTATC ACTGCTCGAG 
GCAGGACGAC CGGAGGACAA GCTGGAGTTC ATGTTCCGGC TGTACGACAC GGATAGCAAC 
GGAGTCCTGG ACACGGCCGA AATGGACGCC ATTGTTAACC AGATGATGGC CGTGGCCGAA 
TACCTCGGCT GGGATGTCAG CGAACTGCGT CCGATACTGC AAGAGATGAT GGTTGAAATC 
GACTACGATG CCGATGGCAC GGTGTCCTTG GACGAGTGGC AGCGAGGCGG GATGACTACG 
ATTCCACTGC TGGTGCTCCT GGGTGTCGAC AGCACCACGC TCAAGGAGGA TGGCATCCAC 
GTGTGGCGCC TGAAGCACTT CAGCAAGCCG GCGTACTGCA ATCTCTGCCT CAACATGTTG 
GTGGGTCTCG GCAAGAAGGG CCTCTGCTGT GTGCTTTGCA AGTACACCGT CCATGAGCGA 
TGCGTGCAAC ATGCTCCCGC GTCCTGCATC ACCACCTACG TCAAGTCCAA GAAACCAAAG 
TGCGGCGGAG ACCTACTGCA TCACTGGGTG GAGGGCAACT GCTATGGACG GTGCTCCAAG 
TGCCGCAAAA GGATAAAGGC CTACCACGGA ATCACAGGAC TTACCTGCCG CTGGTGTCAC 
ATGATGCTCC ACAACCGCTG CGCCTCGTCG GTGAAGAAGG AGTGCACCTT GGGCGAATAC 
TCCGAGCTAA TTGTCCCGCC CACGGCCATC TGCCCGGCGG TACTCGACCG CCAGCGATCC 
GTGAATCAGG CTCACAAGGC CACGCACTTC CAAATCACGC CGCCGGACGA GCTGAGCTGC 
CCGCTGCTGG TGTTCGTCAA TCCGAAGAGC GGCGGTCGCC AGGGGGATCG CATCCTGCGG 
AAGTTCCAGT ACATGCTCAA TCCGCGCCAG GTGTACGACC TGTCCAAGGG CGGGCCCAAG 
GAGGGACTCA CACTCTTCAA GGACCTGCCT CGCTTCAGGG TCATTTGCTG TGGCGGCGAC 
GGCACCGTCG GCTGGGTTTT GGAAGCCATG GACTCCATAG AGTTGGCCAG CCAGCCGGCC 
ATCGGAGTGA TTCCCCTGGG AACGGGCAAC GACCTGGCTC GCTGTCTCCG CTGGGGCGGT 
GGATACGAGG GCGAAAACAT CCCCAAACTG ATGGACAAGT TTCGAAGAGC CTCCACCGTC 
ATGCTGGACC GCTGGAGCAT CGAGGTGACC AATACTCCGC ACAGCGATGA CATGCGACCC 
AAGGTGACGC TGCACTCGAA CATGCAGAAG GTGATTGAGC TGTCGCAAAG TGTTGTGGTC 
GACAAATCGC TGATGGAACG CTTCGAGGAA ATCCAACGGC AGAGCAAGCA GGTGGCCACG 
TCCATGGGCA CGGCGGCCTC AAGCACATCG ATTATGATGG CCAGCAAGAC GGAAACGGAA 
ATGGAAACGA TGGCCACGAT GGAGTTCGGC AGCAGCACGA CCACCACCAA CAGAACGACC 
ACGACCAAGA GCATTTCGAT GTCCACGTTC GAGACGCAGT GCCTGCAACA GACCCTGCGG 
ACAGCGATGA GCAGCAGCAG CAGCAACACG AGCAGCGGCA GTCCGTGCAA CGGCAACCAG 
GATGCGGAAA CGGAAGTAGA TTCCCATGCC GCTGCCGCTG CCGATGTCCG GGAGAAGTCC 
GTGCCCAGGA GGTCGGGTGA AACGGAAAAG CAATCACTTG AGACGCTGCT GCTGCAGCAC 
AAACAGCAGA TGCAACAACA GCAGCAGCAA CAGCAGCAAG GAGTCACATC ACTAGCTGTC 
GAGGAAGCTG CAACAGCAAC GCCAGTCGGG AGTAATCAAA GCGATAATAG TAGCCAGCGC 
AATAAGCAAA ACAACATCCT TAAACAGCAA ATTACATTGT CATTGGACTT GTCCGACCAT 
GAGGACGAGC CCAAGGACGA TGGCGGTGGC GCTGGCGATG GTACCAAGAG CAACGGCAAC 
AGCATTCCGG CAACACCAGC CACACCAATC ACACCCACCA CTCCGAATGC CGCATCAAGT 
GTGCTGCAGC AACAACAGCA ACAGCATCTC CAGTTCGAGC AGCAACAGAA GCCCATCAAA 
GTGCAATCCG ACAAGGACTG CACGGTGCCC TACAACATAA TCAACAATTA TTTCTCCGTC 
GGCGTGGACG CCGCCATCTG CGTCAAGTTC CACCTGGAGC GTGAGAAGAA CCCGCACAAA 
TTCAACAGCC GCATGAAGAA CAAGCTGTGG TACTTCGAGT ATGCAACATC CGAGACATTT 
GCGGCATCCT GCAAGAATCT CCACGAGAGC ATCGAGATTG TGTGCGATGG CGTGGCCCTG 
GACTTGGCCA ACGGACCCCA CTTGCAGGGA GTGGCTCTGC TCAACATCCC GTACACACAC 
GGCGGTTCCA ACCTGTGGGG CGAGCACCTG TCTCAGAAAC GGATCCGCAA GAGTGCCGGC 
CCCTTCGGCA AGAGTAAGAA ACTACGCGCC GGCGACAAGG AGTTCTCCGC CACCAGCTTC 
AATTCCGTGG ACCTCTCGGT GGCCATTCAG GACTTTGGAG ATCGGCTCAT CGAGGTAATT 
GGGCTGGAGA ACTGCCTGCA CATGGGCCAG GTGAGGACTG GACTTCGCGC GTCGGGACGC 
CGCCTGGCCC AGTGCAGCGA AGTGATCATC AAGACGAAGA AAACATTTCC CATGCAGATA 
GATGGCGAGC CCTGGATGCA GATGCCCTGC ACGATCAAGG TGACCCACAA GAACCAGGTG 
CCCATGCTGA TGGCACCGCG GTCGGAAAAG GGACGCGGAT TCTTCAACCT GCTGTGCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dgk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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