sced cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sced
Entrez Gene ID	35564
Full Name	scrambled
Synonyms	CG3273,CT9143,Dmel\CG3273,Sced
General protein information	Preferred Names scrambled Names CG3273 gene product from transcript CG3273-RA CG3273-PA CG3273-PB sced-PA sced-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42C2-42C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273799.1	NP_001260728.1	scrambled, isoform B
NM_136375.2	NP_610219.2	scrambled, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sced gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sced cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09034	NM_001273799.1 Latest version!	Drosophila melanogaster scrambled (sced), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09034	NM_136375.2 Latest version!	Drosophila melanogaster scrambled (sced), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sced ( NM_001273799.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260728.1 Drosophila melanogaster scrambled (sced), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09034
Clone ID Related Accession (Same CDS sequence)	NM_001273799.1 , NM_136375.2
Accession Version	NM_001273799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scrambled, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACA ACGAAATCTC CATGACTATG GACGCCAAGC TGGATACACA CGATAAGCGA 
CCAGTGGCGC TGCGTTACAA GACATGGACC CGCTGCCAAG TGAAAATAGA GGCCATTAAG 
TGTGTGCCCT GCTTCCTGCG GCTCAGTTTC CAGGCCATGG AGACCGAGTC TCACTCCCAA 
GAGTGTCACC CCGCTAAACC CATTAGCGTC TCCGTAAACG TTCCGGGCGT CAGCTTGAGC 
CTGGCTACCT CGCGCACGAA GCAGCACTCG TACGGGCTGT TTTGGACGCA AAATCGGCGG 
GACTGCTTCA TCTATTTCGC CCTGGAAACG GAGACATCCT GCCGACGTCA CATGAAATGG 
ATGCGGAAGT CGATTAAGAA TCTGGAACTT TATCGCCAGG TCTTTCTAGA ACAGCGCCGG 
CTGAGCAGGG GTCCCGGAGG TCTGGGCCCA CTGCCTAACC TGCCGGAAAC CCTCGACGCT 
AGCGCTCTCT CTCCGCGAGT CTCGCAAATA TACGAGGAGA TTTTTGACGG CAGCCGCATC 
TCCCGCGTCT CAATCGCATC CGGCATTTAC GAAGAGATGA AGCCGTCAGT CATGGAGACT 
CCTTCACCGC CACCGCTGCC CGCACATCCT TACCGTCAGC GAATAAACAC TTTTGAGTGC 
GCCCAGCTTG GCGAGATTGC GCGGTCCAGC ACCAATCCCG AATCGGAGCT GGCCAAGAAG 
AAGAAATACA AGAACATGCT GGACAACCTG TTCGGGAGTT CACGTCAAAA GCGTTCCGGC 
AGCACCAGCG AGGTGGCCAA TGAACCCAGC GATGAGGTCC TGCCACTGTA TGAGAATGCC 
CCCACGCCGG AGCCTGTGCT TCCGGTAAAG CGCACATCCC AGTTGAACAA GTCTCAGCGT 
AATAGCTTCT CAAGTCCGGA CTTGTCCAAA CTCAATCTGC TGGACACCTT TGGAGAATAC 
TTCGAGGCCC AGAACCATGA GTCGAGCATG GAGATAAACA TTAGTCTGGA TGAGTCGGTC 
ATTGAGCCGA TCTCTCGCAA TGAGATTATT GCGCAAATAT CTAGCCAATT GGGCAAAGCA 
GACTCTTTGG AAAGTCTGAA TGTTTCGGAG CAACTTCCGT ACAACTTCAA CTTTTCCGCT 
TGTAACACGT CAACCATCAA TCTCATTGGA TCGCATGGAG CTGTTCCGCA GAGTAATCAG 
CTAAAGAAAA ATAAGATACT GCACGAAGAC CTTACGGGAT ACTGTGTAAT GGCACCCATT 
TTAAAGGCGA CGGTAAAAGA TTTGCCACCA CAGCCAGGCG GCAGTACAGT GTCCACATCC 
TCCGGATATT CCACGGGCTC CTACTGCTCC TCCACCAGCA GCTGTAACAC GGAGGACCAG 
CCCACCAAGA CGCAGATCGC CAATGAGAGT GACATATACG AAGTCATGCA TGGAACCTCT 
CTGAACAGTA CCGTCGGAGC GGCTGCAGCT GGATTTGCGG AACACCTCTA CGAGAACCTT 
TTGGCTGTGA AGGCCGCACA GGAGCTGGAG GCGCAGCCTG TCGGCTTTGG ACTGAGCACA 
AGCACACCCA CCGAATCTCC CCTGGCGCCG GCCTTGCCGC AAAAGGGAAC GCCCAAGGAC 
AAAACTTTGC AACAGAAGCC AGAGGAGGAA AACTACTACC AGACACCAAG AAAGTCGATT 
ATCAGTGTGG ACGACAAGAT ACCCTCCTAT TATCCGAACA GCTGCGACAC ACTCAAGTCA 
AAGCGACGCA GTCCCACCGA ACATGGACCT AGTTTTAGGC ACTTAGTCAG TTCCAAGTTG 
CGACGTGAAC GAAAGGAGAA CCTCTACATT TCCTCGCCCC AGCAAATCAT TGAGCAGCGT 
CACAAGGCGG CCACCATCTC ATCAGCATCA GTATCAGCCA AGACTAAAAG CTCAACCAAA 
AAGACTGCCG CCCACAAAAT TTCTGACAGT GTTTATGCAG TCACCCATCC GGTAAAGCGT 
CCGAACAGCA CCAATAGCAA CAATACCCAC CAAAGCAACA ATAACAATAA CAATGAAGGC 
GTGGATGACG AACTGCTGCA CTTGACCGTT TTGAAGAACA TTGACTTTAA GTTTGATGAT 
AGCCAAGCAT CTGAGGATTC CTGCAAGGCG AAAGCATCTG CTCTTCCCGT AGCGAACGAA 
TGCTTTGTAC AGCCAAATCT GGAGAAGGAG TACGAGGATC GCTTTCAAGC GGCGGAAAAG 
GCTAGTCGTC TTTTGCAAAA GTATGCGACG CTGGCCAAAA TCGGGCATTC GCCCAGCAAG 
GCGTTGCCCG CACCAGCGCG TCTGGAACCC ATGATTCAGT CTGTGGCCAC GTCCAATGAG 
TTGCAGCCCG AGGGAGGCAC CATGAAAAAG TTCGCCTCCC TACCACGCTT CAAGAAAATT 
GACTTTTCGC CGCTGAAGAT GAGACTCAAT AGTGTCCTCC AGAGAAATCC ACCTTCGCCG 
CAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260728.1
CDS	187..2655
Translation	MDHNEISMTMDAKLDTHDKRPVALRYKTWTRCQVKIEAIKCVPCFLRLSFQAMETESHSQECHPAKPISVSVNVPGVSLSLATSRTKQHSYGLFWTQNRRDCFIYFALETETSCRRHMKWMRKSIKNLELYRQVFLEQRRLSRGPGGLGPLPNLPETLDASALSPRVSQIYEEIFDGSRISRVSIASGIYEEMKPSVMETPSPPPLPAHPYRQRINTFECAQLGEIARSSTNPESELAKKKKYKNMLDNLFGSSRQKRSGSTSEVANEPSDEVLPLYENAPTPEPVLPVKRTSQLNKSQRNSFSSPDLSKLNLLDTFGEYFEAQNHESSMEINISLDESVIEPISRNEIIAQISSQLGKADSLESLNVSEQLPYNFNFSACNTSTINLIGSHGAVPQSNQLKKNKILHEDLTGYCVMAPILKATVKDLPPQPGGSTVSTSSGYSTGSYCSSTSSCNTEDQPTKTQIANESDIYEVMHGTSLNSTVGAAAAGFAEHLYENLLAVKAAQELEAQPVGFGLSTSTPTESPLAPALPQKGTPKDKTLQQKPEEENYYQTPRKSIISVDDKIPSYYPNSCDTLKSKRRSPTEHGPSFRHLVSSKLRRERKENLYISSPQQIIEQRHKAATISSASVSAKTKSSTKKTAAHKISDSVYAVTHPVKRPNSTNSNNTHQSNNNNNNEGVDDELLHLTVLKNIDFKFDDSQASEDSCKAKASALPVANECFVQPNLEKEYEDRFQAAEKASRLLQKYATLAKIGHSPSKALPAPARLEPMIQSVATSNELQPEGGTMKKFASLPRFKKIDFSPLKMRLNSVLQRNPPSPQH

Target ORF information:

RefSeq Version	NM_001273799.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scrambled (sced), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273799.1

ORF Insert Sequence:

ATGGACCACA ACGAAATCTC CATGACTATG GACGCCAAGC TGGATACACA CGATAAGCGA 
CCAGTGGCGC TGCGTTACAA GACATGGACC CGCTGCCAAG TGAAAATAGA GGCCATTAAG 
TGTGTGCCCT GCTTCCTGCG GCTCAGTTTC CAGGCCATGG AGACCGAGTC TCACTCCCAA 
GAGTGTCACC CCGCTAAACC CATTAGCGTC TCCGTAAACG TTCCGGGCGT CAGCTTGAGC 
CTGGCTACCT CGCGCACGAA GCAGCACTCG TACGGGCTGT TTTGGACGCA AAATCGGCGG 
GACTGCTTCA TCTATTTCGC CCTGGAAACG GAGACATCCT GCCGACGTCA CATGAAATGG 
ATGCGGAAGT CGATTAAGAA TCTGGAACTT TATCGCCAGG TCTTTCTAGA ACAGCGCCGG 
CTGAGCAGGG GTCCCGGAGG TCTGGGCCCA CTGCCTAACC TGCCGGAAAC CCTCGACGCT 
AGCGCTCTCT CTCCGCGAGT CTCGCAAATA TACGAGGAGA TTTTTGACGG CAGCCGCATC 
TCCCGCGTCT CAATCGCATC CGGCATTTAC GAAGAGATGA AGCCGTCAGT CATGGAGACT 
CCTTCACCGC CACCGCTGCC CGCACATCCT TACCGTCAGC GAATAAACAC TTTTGAGTGC 
GCCCAGCTTG GCGAGATTGC GCGGTCCAGC ACCAATCCCG AATCGGAGCT GGCCAAGAAG 
AAGAAATACA AGAACATGCT GGACAACCTG TTCGGGAGTT CACGTCAAAA GCGTTCCGGC 
AGCACCAGCG AGGTGGCCAA TGAACCCAGC GATGAGGTCC TGCCACTGTA TGAGAATGCC 
CCCACGCCGG AGCCTGTGCT TCCGGTAAAG CGCACATCCC AGTTGAACAA GTCTCAGCGT 
AATAGCTTCT CAAGTCCGGA CTTGTCCAAA CTCAATCTGC TGGACACCTT TGGAGAATAC 
TTCGAGGCCC AGAACCATGA GTCGAGCATG GAGATAAACA TTAGTCTGGA TGAGTCGGTC 
ATTGAGCCGA TCTCTCGCAA TGAGATTATT GCGCAAATAT CTAGCCAATT GGGCAAAGCA 
GACTCTTTGG AAAGTCTGAA TGTTTCGGAG CAACTTCCGT ACAACTTCAA CTTTTCCGCT 
TGTAACACGT CAACCATCAA TCTCATTGGA TCGCATGGAG CTGTTCCGCA GAGTAATCAG 
CTAAAGAAAA ATAAGATACT GCACGAAGAC CTTACGGGAT ACTGTGTAAT GGCACCCATT 
TTAAAGGCGA CGGTAAAAGA TTTGCCACCA CAGCCAGGCG GCAGTACAGT GTCCACATCC 
TCCGGATATT CCACGGGCTC CTACTGCTCC TCCACCAGCA GCTGTAACAC GGAGGACCAG 
CCCACCAAGA CGCAGATCGC CAATGAGAGT GACATATACG AAGTCATGCA TGGAACCTCT 
CTGAACAGTA CCGTCGGAGC GGCTGCAGCT GGATTTGCGG AACACCTCTA CGAGAACCTT 
TTGGCTGTGA AGGCCGCACA GGAGCTGGAG GCGCAGCCTG TCGGCTTTGG ACTGAGCACA 
AGCACACCCA CCGAATCTCC CCTGGCGCCG GCCTTGCCGC AAAAGGGAAC GCCCAAGGAC 
AAAACTTTGC AACAGAAGCC AGAGGAGGAA AACTACTACC AGACACCAAG AAAGTCGATT 
ATCAGTGTGG ACGACAAGAT ACCCTCCTAT TATCCGAACA GCTGCGACAC ACTCAAGTCA 
AAGCGACGCA GTCCCACCGA ACATGGACCT AGTTTTAGGC ACTTAGTCAG TTCCAAGTTG 
CGACGTGAAC GAAAGGAGAA CCTCTACATT TCCTCGCCCC AGCAAATCAT TGAGCAGCGT 
CACAAGGCGG CCACCATCTC ATCAGCATCA GTATCAGCCA AGACTAAAAG CTCAACCAAA 
AAGACTGCCG CCCACAAAAT TTCTGACAGT GTTTATGCAG TCACCCATCC GGTAAAGCGT 
CCGAACAGCA CCAATAGCAA CAATACCCAC CAAAGCAACA ATAACAATAA CAATGAAGGC 
GTGGATGACG AACTGCTGCA CTTGACCGTT TTGAAGAACA TTGACTTTAA GTTTGATGAT 
AGCCAAGCAT CTGAGGATTC CTGCAAGGCG AAAGCATCTG CTCTTCCCGT AGCGAACGAA 
TGCTTTGTAC AGCCAAATCT GGAGAAGGAG TACGAGGATC GCTTTCAAGC GGCGGAAAAG 
GCTAGTCGTC TTTTGCAAAA GTATGCGACG CTGGCCAAAA TCGGGCATTC GCCCAGCAAG 
GCGTTGCCCG CACCAGCGCG TCTGGAACCC ATGATTCAGT CTGTGGCCAC GTCCAATGAG 
TTGCAGCCCG AGGGAGGCAC CATGAAAAAG TTCGCCTCCC TACCACGCTT CAAGAAAATT 
GACTTTTCGC CGCTGAAGAT GAGACTCAAT AGTGTCCTCC AGAGAAATCC ACCTTCGCCG 
CAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sced ( NM_136375.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610219.2 Drosophila melanogaster scrambled (sced), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09034
Clone ID Related Accession (Same CDS sequence)	NM_001273799.1 , NM_136375.2
Accession Version	NM_136375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scrambled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136375.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACA ACGAAATCTC CATGACTATG GACGCCAAGC TGGATACACA CGATAAGCGA 
CCAGTGGCGC TGCGTTACAA GACATGGACC CGCTGCCAAG TGAAAATAGA GGCCATTAAG 
TGTGTGCCCT GCTTCCTGCG GCTCAGTTTC CAGGCCATGG AGACCGAGTC TCACTCCCAA 
GAGTGTCACC CCGCTAAACC CATTAGCGTC TCCGTAAACG TTCCGGGCGT CAGCTTGAGC 
CTGGCTACCT CGCGCACGAA GCAGCACTCG TACGGGCTGT TTTGGACGCA AAATCGGCGG 
GACTGCTTCA TCTATTTCGC CCTGGAAACG GAGACATCCT GCCGACGTCA CATGAAATGG 
ATGCGGAAGT CGATTAAGAA TCTGGAACTT TATCGCCAGG TCTTTCTAGA ACAGCGCCGG 
CTGAGCAGGG GTCCCGGAGG TCTGGGCCCA CTGCCTAACC TGCCGGAAAC CCTCGACGCT 
AGCGCTCTCT CTCCGCGAGT CTCGCAAATA TACGAGGAGA TTTTTGACGG CAGCCGCATC 
TCCCGCGTCT CAATCGCATC CGGCATTTAC GAAGAGATGA AGCCGTCAGT CATGGAGACT 
CCTTCACCGC CACCGCTGCC CGCACATCCT TACCGTCAGC GAATAAACAC TTTTGAGTGC 
GCCCAGCTTG GCGAGATTGC GCGGTCCAGC ACCAATCCCG AATCGGAGCT GGCCAAGAAG 
AAGAAATACA AGAACATGCT GGACAACCTG TTCGGGAGTT CACGTCAAAA GCGTTCCGGC 
AGCACCAGCG AGGTGGCCAA TGAACCCAGC GATGAGGTCC TGCCACTGTA TGAGAATGCC 
CCCACGCCGG AGCCTGTGCT TCCGGTAAAG CGCACATCCC AGTTGAACAA GTCTCAGCGT 
AATAGCTTCT CAAGTCCGGA CTTGTCCAAA CTCAATCTGC TGGACACCTT TGGAGAATAC 
TTCGAGGCCC AGAACCATGA GTCGAGCATG GAGATAAACA TTAGTCTGGA TGAGTCGGTC 
ATTGAGCCGA TCTCTCGCAA TGAGATTATT GCGCAAATAT CTAGCCAATT GGGCAAAGCA 
GACTCTTTGG AAAGTCTGAA TGTTTCGGAG CAACTTCCGT ACAACTTCAA CTTTTCCGCT 
TGTAACACGT CAACCATCAA TCTCATTGGA TCGCATGGAG CTGTTCCGCA GAGTAATCAG 
CTAAAGAAAA ATAAGATACT GCACGAAGAC CTTACGGGAT ACTGTGTAAT GGCACCCATT 
TTAAAGGCGA CGGTAAAAGA TTTGCCACCA CAGCCAGGCG GCAGTACAGT GTCCACATCC 
TCCGGATATT CCACGGGCTC CTACTGCTCC TCCACCAGCA GCTGTAACAC GGAGGACCAG 
CCCACCAAGA CGCAGATCGC CAATGAGAGT GACATATACG AAGTCATGCA TGGAACCTCT 
CTGAACAGTA CCGTCGGAGC GGCTGCAGCT GGATTTGCGG AACACCTCTA CGAGAACCTT 
TTGGCTGTGA AGGCCGCACA GGAGCTGGAG GCGCAGCCTG TCGGCTTTGG ACTGAGCACA 
AGCACACCCA CCGAATCTCC CCTGGCGCCG GCCTTGCCGC AAAAGGGAAC GCCCAAGGAC 
AAAACTTTGC AACAGAAGCC AGAGGAGGAA AACTACTACC AGACACCAAG AAAGTCGATT 
ATCAGTGTGG ACGACAAGAT ACCCTCCTAT TATCCGAACA GCTGCGACAC ACTCAAGTCA 
AAGCGACGCA GTCCCACCGA ACATGGACCT AGTTTTAGGC ACTTAGTCAG TTCCAAGTTG 
CGACGTGAAC GAAAGGAGAA CCTCTACATT TCCTCGCCCC AGCAAATCAT TGAGCAGCGT 
CACAAGGCGG CCACCATCTC ATCAGCATCA GTATCAGCCA AGACTAAAAG CTCAACCAAA 
AAGACTGCCG CCCACAAAAT TTCTGACAGT GTTTATGCAG TCACCCATCC GGTAAAGCGT 
CCGAACAGCA CCAATAGCAA CAATACCCAC CAAAGCAACA ATAACAATAA CAATGAAGGC 
GTGGATGACG AACTGCTGCA CTTGACCGTT TTGAAGAACA TTGACTTTAA GTTTGATGAT 
AGCCAAGCAT CTGAGGATTC CTGCAAGGCG AAAGCATCTG CTCTTCCCGT AGCGAACGAA 
TGCTTTGTAC AGCCAAATCT GGAGAAGGAG TACGAGGATC GCTTTCAAGC GGCGGAAAAG 
GCTAGTCGTC TTTTGCAAAA GTATGCGACG CTGGCCAAAA TCGGGCATTC GCCCAGCAAG 
GCGTTGCCCG CACCAGCGCG TCTGGAACCC ATGATTCAGT CTGTGGCCAC GTCCAATGAG 
TTGCAGCCCG AGGGAGGCAC CATGAAAAAG TTCGCCTCCC TACCACGCTT CAAGAAAATT 
GACTTTTCGC CGCTGAAGAT GAGACTCAAT AGTGTCCTCC AGAGAAATCC ACCTTCGCCG 
CAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610219.2
CDS	187..2655
Translation	MDHNEISMTMDAKLDTHDKRPVALRYKTWTRCQVKIEAIKCVPCFLRLSFQAMETESHSQECHPAKPISVSVNVPGVSLSLATSRTKQHSYGLFWTQNRRDCFIYFALETETSCRRHMKWMRKSIKNLELYRQVFLEQRRLSRGPGGLGPLPNLPETLDASALSPRVSQIYEEIFDGSRISRVSIASGIYEEMKPSVMETPSPPPLPAHPYRQRINTFECAQLGEIARSSTNPESELAKKKKYKNMLDNLFGSSRQKRSGSTSEVANEPSDEVLPLYENAPTPEPVLPVKRTSQLNKSQRNSFSSPDLSKLNLLDTFGEYFEAQNHESSMEINISLDESVIEPISRNEIIAQISSQLGKADSLESLNVSEQLPYNFNFSACNTSTINLIGSHGAVPQSNQLKKNKILHEDLTGYCVMAPILKATVKDLPPQPGGSTVSTSSGYSTGSYCSSTSSCNTEDQPTKTQIANESDIYEVMHGTSLNSTVGAAAAGFAEHLYENLLAVKAAQELEAQPVGFGLSTSTPTESPLAPALPQKGTPKDKTLQQKPEEENYYQTPRKSIISVDDKIPSYYPNSCDTLKSKRRSPTEHGPSFRHLVSSKLRRERKENLYISSPQQIIEQRHKAATISSASVSAKTKSSTKKTAAHKISDSVYAVTHPVKRPNSTNSNNTHQSNNNNNNEGVDDELLHLTVLKNIDFKFDDSQASEDSCKAKASALPVANECFVQPNLEKEYEDRFQAAEKASRLLQKYATLAKIGHSPSKALPAPARLEPMIQSVATSNELQPEGGTMKKFASLPRFKKIDFSPLKMRLNSVLQRNPPSPQH

Target ORF information:

RefSeq Version	NM_136375.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scrambled (sced), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136375.2

ORF Insert Sequence:

ATGGACCACA ACGAAATCTC CATGACTATG GACGCCAAGC TGGATACACA CGATAAGCGA 
CCAGTGGCGC TGCGTTACAA GACATGGACC CGCTGCCAAG TGAAAATAGA GGCCATTAAG 
TGTGTGCCCT GCTTCCTGCG GCTCAGTTTC CAGGCCATGG AGACCGAGTC TCACTCCCAA 
GAGTGTCACC CCGCTAAACC CATTAGCGTC TCCGTAAACG TTCCGGGCGT CAGCTTGAGC 
CTGGCTACCT CGCGCACGAA GCAGCACTCG TACGGGCTGT TTTGGACGCA AAATCGGCGG 
GACTGCTTCA TCTATTTCGC CCTGGAAACG GAGACATCCT GCCGACGTCA CATGAAATGG 
ATGCGGAAGT CGATTAAGAA TCTGGAACTT TATCGCCAGG TCTTTCTAGA ACAGCGCCGG 
CTGAGCAGGG GTCCCGGAGG TCTGGGCCCA CTGCCTAACC TGCCGGAAAC CCTCGACGCT 
AGCGCTCTCT CTCCGCGAGT CTCGCAAATA TACGAGGAGA TTTTTGACGG CAGCCGCATC 
TCCCGCGTCT CAATCGCATC CGGCATTTAC GAAGAGATGA AGCCGTCAGT CATGGAGACT 
CCTTCACCGC CACCGCTGCC CGCACATCCT TACCGTCAGC GAATAAACAC TTTTGAGTGC 
GCCCAGCTTG GCGAGATTGC GCGGTCCAGC ACCAATCCCG AATCGGAGCT GGCCAAGAAG 
AAGAAATACA AGAACATGCT GGACAACCTG TTCGGGAGTT CACGTCAAAA GCGTTCCGGC 
AGCACCAGCG AGGTGGCCAA TGAACCCAGC GATGAGGTCC TGCCACTGTA TGAGAATGCC 
CCCACGCCGG AGCCTGTGCT TCCGGTAAAG CGCACATCCC AGTTGAACAA GTCTCAGCGT 
AATAGCTTCT CAAGTCCGGA CTTGTCCAAA CTCAATCTGC TGGACACCTT TGGAGAATAC 
TTCGAGGCCC AGAACCATGA GTCGAGCATG GAGATAAACA TTAGTCTGGA TGAGTCGGTC 
ATTGAGCCGA TCTCTCGCAA TGAGATTATT GCGCAAATAT CTAGCCAATT GGGCAAAGCA 
GACTCTTTGG AAAGTCTGAA TGTTTCGGAG CAACTTCCGT ACAACTTCAA CTTTTCCGCT 
TGTAACACGT CAACCATCAA TCTCATTGGA TCGCATGGAG CTGTTCCGCA GAGTAATCAG 
CTAAAGAAAA ATAAGATACT GCACGAAGAC CTTACGGGAT ACTGTGTAAT GGCACCCATT 
TTAAAGGCGA CGGTAAAAGA TTTGCCACCA CAGCCAGGCG GCAGTACAGT GTCCACATCC 
TCCGGATATT CCACGGGCTC CTACTGCTCC TCCACCAGCA GCTGTAACAC GGAGGACCAG 
CCCACCAAGA CGCAGATCGC CAATGAGAGT GACATATACG AAGTCATGCA TGGAACCTCT 
CTGAACAGTA CCGTCGGAGC GGCTGCAGCT GGATTTGCGG AACACCTCTA CGAGAACCTT 
TTGGCTGTGA AGGCCGCACA GGAGCTGGAG GCGCAGCCTG TCGGCTTTGG ACTGAGCACA 
AGCACACCCA CCGAATCTCC CCTGGCGCCG GCCTTGCCGC AAAAGGGAAC GCCCAAGGAC 
AAAACTTTGC AACAGAAGCC AGAGGAGGAA AACTACTACC AGACACCAAG AAAGTCGATT 
ATCAGTGTGG ACGACAAGAT ACCCTCCTAT TATCCGAACA GCTGCGACAC ACTCAAGTCA 
AAGCGACGCA GTCCCACCGA ACATGGACCT AGTTTTAGGC ACTTAGTCAG TTCCAAGTTG 
CGACGTGAAC GAAAGGAGAA CCTCTACATT TCCTCGCCCC AGCAAATCAT TGAGCAGCGT 
CACAAGGCGG CCACCATCTC ATCAGCATCA GTATCAGCCA AGACTAAAAG CTCAACCAAA 
AAGACTGCCG CCCACAAAAT TTCTGACAGT GTTTATGCAG TCACCCATCC GGTAAAGCGT 
CCGAACAGCA CCAATAGCAA CAATACCCAC CAAAGCAACA ATAACAATAA CAATGAAGGC 
GTGGATGACG AACTGCTGCA CTTGACCGTT TTGAAGAACA TTGACTTTAA GTTTGATGAT 
AGCCAAGCAT CTGAGGATTC CTGCAAGGCG AAAGCATCTG CTCTTCCCGT AGCGAACGAA 
TGCTTTGTAC AGCCAAATCT GGAGAAGGAG TACGAGGATC GCTTTCAAGC GGCGGAAAAG 
GCTAGTCGTC TTTTGCAAAA GTATGCGACG CTGGCCAAAA TCGGGCATTC GCCCAGCAAG 
GCGTTGCCCG CACCAGCGCG TCTGGAACCC ATGATTCAGT CTGTGGCCAC GTCCAATGAG 
TTGCAGCCCG AGGGAGGCAC CATGAAAAAG TTCGCCTCCC TACCACGCTT CAAGAAAATT 
GACTTTTCGC CGCTGAAGAT GAGACTCAAT AGTGTCCTCC AGAGAAATCC ACCTTCGCCG 
CAGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sced cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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