geminin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	geminin
Entrez Gene ID	35563
Full Name	CG3183 gene product from transcript CG3183-RA
Synonyms	CG3183,Dm Geminin,Dm geminin,Dmel\CG3183,Gem,Geminin,anon-WO0257455.1,gem,l(2)k14019,v(2)k09107
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42C2-42C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_136374.4	NP_610218.1	geminin, isoform A
NM_001273798.1	NP_001260727.1	geminin, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the absence of geminin, limited pre-replicative complex assembly was restricted to the heterochromatin by elevated cyclin A-CDK activity.
Title: Preferential re-replication of Drosophila heterochromatin in the absence of geminin.

Gem and Brm act antagonistically to modulate the EGFR-Ras-MAPK signaling pathway, by affecting Mek levels during Drosophila development
Title: Geminin and Brahma act antagonistically to regulate EGFR-Ras-MAPK signaling in Drosophila.

Down-regulation of APC/C activity results in stabilization of Geminin protein and blocks endocycle progression.
Title: The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles.

In cycling cells, Dup destruction is coupled to DNA replication and that increased levels of Gem balance elevated Dup levels to prevent pre-replicative complex reformation when Dup degradation fails.
Title: Levels of the origin-binding protein Double parked and its inhibitor Geminin increase in response to replication stress.

The following geminin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the geminin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09033	NM_136374.4 Latest version!	Drosophila melanogaster geminin, transcript variant A (geminin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09033	NM_001273798.1 Latest version!	Drosophila melanogaster geminin, transcript variant B (geminin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

geminin ( NM_136374.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610218.1 Drosophila melanogaster geminin, transcript variant A (geminin), mRNA.

Price & Availability↑

CloneID	OFa09033
Clone ID Related Accession (Same CDS sequence)	NM_136374.4 , NM_001273798.1
Accession Version	NM_136374.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	geminin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136374.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGA GCGCTGCCAG GGTCTACATC CAAGTCGAGA CAGAGGCCGA GCAGAAGGAG 
CACCTGAAGC AGCAGAGACA GACCCTGAAA CCCTTGCAGG GCAACGTAAA CGATAAGGAG 
AACCTCACTG GTTCAGGGCG CGCCTCCATC GTGGATCAGC TGAGTCGTCT GAAGGCCGGC 
GTCCAAGTCG CGCCCAAGTA CGGCAAGCGC AAATGCGTGG ACACGGCGCC CGCGATTTCC 
ACAAAGGATG CGGATACCCA GACTGATGCT GAGGCCGTTC CGCTTGGCAA TGCTGACAAG 
CCCATCACGG CAGAGGATCT CACTAGCACA GCCGAACCCG GCGAGAACTA CTACAAGCTG 
CTGGCCGAGC AGCGGCGACT GGCGCTGGAG GACTCGCTCA CCGAAAACCG CCATCTGCAC 
GAGCGCATCG AGGGGCTTGA GGAGGAGATG GACACTATGC GCCAGGAACT GGACGAGGCC 
AAGAATTTGG TGGAGGTCCT GAAGGAGATC TGCGAAGAGG ACAACAGCGA GGTGGAGGAG 
GACGACACTA CGGGTGACGA GGACAAGGTC AACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610218.1
CDS	295..873
Translation	MSSSAARVYIQVETEAEQKEHLKQQRQTLKPLQGNVNDKENLTGSGRASIVDQLSRLKAGVQVAPKYGKRKCVDTAPAISTKDADTQTDAEAVPLGNADKPITAEDLTSTAEPGENYYKLLAEQRRLALEDSLTENRHLHERIEGLEEEMDTMRQELDEAKNLVEVLKEICEEDNSEVEEDDTTGDEDKVNA

Target ORF information:

RefSeq Version	NM_136374.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster geminin, transcript variant A (geminin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136374.4

ORF Insert Sequence:

ATGTCTTCGA GCGCTGCCAG GGTCTACATC CAAGTCGAGA CAGAGGCCGA GCAGAAGGAG 
CACCTGAAGC AGCAGAGACA GACCCTGAAA CCCTTGCAGG GCAACGTAAA CGATAAGGAG 
AACCTCACTG GTTCAGGGCG CGCCTCCATC GTGGATCAGC TGAGTCGTCT GAAGGCCGGC 
GTCCAAGTCG CGCCCAAGTA CGGCAAGCGC AAATGCGTGG ACACGGCGCC CGCGATTTCC 
ACAAAGGATG CGGATACCCA GACTGATGCT GAGGCCGTTC CGCTTGGCAA TGCTGACAAG 
CCCATCACGG CAGAGGATCT CACTAGCACA GCCGAACCCG GCGAGAACTA CTACAAGCTG 
CTGGCCGAGC AGCGGCGACT GGCGCTGGAG GACTCGCTCA CCGAAAACCG CCATCTGCAC 
GAGCGCATCG AGGGGCTTGA GGAGGAGATG GACACTATGC GCCAGGAACT GGACGAGGCC 
AAGAATTTGG TGGAGGTCCT GAAGGAGATC TGCGAAGAGG ACAACAGCGA GGTGGAGGAG 
GACGACACTA CGGGTGACGA GGACAAGGTC AACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

geminin ( NM_001273798.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260727.1 Drosophila melanogaster geminin, transcript variant B (geminin), mRNA.

Price & Availability↑

CloneID	OFa09033
Clone ID Related Accession (Same CDS sequence)	NM_136374.4 , NM_001273798.1
Accession Version	NM_001273798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	geminin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGA GCGCTGCCAG GGTCTACATC CAAGTCGAGA CAGAGGCCGA GCAGAAGGAG 
CACCTGAAGC AGCAGAGACA GACCCTGAAA CCCTTGCAGG GCAACGTAAA CGATAAGGAG 
AACCTCACTG GTTCAGGGCG CGCCTCCATC GTGGATCAGC TGAGTCGTCT GAAGGCCGGC 
GTCCAAGTCG CGCCCAAGTA CGGCAAGCGC AAATGCGTGG ACACGGCGCC CGCGATTTCC 
ACAAAGGATG CGGATACCCA GACTGATGCT GAGGCCGTTC CGCTTGGCAA TGCTGACAAG 
CCCATCACGG CAGAGGATCT CACTAGCACA GCCGAACCCG GCGAGAACTA CTACAAGCTG 
CTGGCCGAGC AGCGGCGACT GGCGCTGGAG GACTCGCTCA CCGAAAACCG CCATCTGCAC 
GAGCGCATCG AGGGGCTTGA GGAGGAGATG GACACTATGC GCCAGGAACT GGACGAGGCC 
AAGAATTTGG TGGAGGTCCT GAAGGAGATC TGCGAAGAGG ACAACAGCGA GGTGGAGGAG 
GACGACACTA CGGGTGACGA GGACAAGGTC AACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260727.1
CDS	173..751
Translation	MSSSAARVYIQVETEAEQKEHLKQQRQTLKPLQGNVNDKENLTGSGRASIVDQLSRLKAGVQVAPKYGKRKCVDTAPAISTKDADTQTDAEAVPLGNADKPITAEDLTSTAEPGENYYKLLAEQRRLALEDSLTENRHLHERIEGLEEEMDTMRQELDEAKNLVEVLKEICEEDNSEVEEDDTTGDEDKVNA

Target ORF information:

RefSeq Version	NM_001273798.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster geminin, transcript variant B (geminin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273798.1

ORF Insert Sequence:

ATGTCTTCGA GCGCTGCCAG GGTCTACATC CAAGTCGAGA CAGAGGCCGA GCAGAAGGAG 
CACCTGAAGC AGCAGAGACA GACCCTGAAA CCCTTGCAGG GCAACGTAAA CGATAAGGAG 
AACCTCACTG GTTCAGGGCG CGCCTCCATC GTGGATCAGC TGAGTCGTCT GAAGGCCGGC 
GTCCAAGTCG CGCCCAAGTA CGGCAAGCGC AAATGCGTGG ACACGGCGCC CGCGATTTCC 
ACAAAGGATG CGGATACCCA GACTGATGCT GAGGCCGTTC CGCTTGGCAA TGCTGACAAG 
CCCATCACGG CAGAGGATCT CACTAGCACA GCCGAACCCG GCGAGAACTA CTACAAGCTG 
CTGGCCGAGC AGCGGCGACT GGCGCTGGAG GACTCGCTCA CCGAAAACCG CCATCTGCAC 
GAGCGCATCG AGGGGCTTGA GGAGGAGATG GACACTATGC GCCAGGAACT GGACGAGGCC 
AAGAATTTGG TGGAGGTCCT GAAGGAGATC TGCGAAGAGG ACAACAGCGA GGTGGAGGAG 
GACGACACTA CGGGTGACGA GGACAAGGTC AACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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geminin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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