nolo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nolo
Entrez Gene ID	35424
Full Name	no long nerve cord
Synonyms	CG13236,CG2122,CG2131,CG31619,CT6940,Dmel\CG31619
General protein information	Preferred Names no long nerve cord Names CG31619 gene product from transcript CG31619-RE CG31619-PA CG31619-PB CG31619-PC CG31619-PD CG31619-PE CG31619-PF CG31619-PG nolo-PA nolo-PB nolo-PC nolo-PD nolo-PE nolo-PF nolo-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39F1-39F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165397.3	NP_724360.1	no long nerve cord, isoform B
NM_001259199.2	NP_001246128.1	no long nerve cord, isoform E
NM_001299197.1	NP_001286126.1	no long nerve cord, isoform F
NM_001042909.3	NP_001036374.1	no long nerve cord, isoform C
NM_165396.3	NP_724359.1	no long nerve cord, isoform A
NM_001169569.3	NP_001163040.1	no long nerve cord, isoform D
NM_001299198.1	NP_001286127.1	no long nerve cord, isoform G

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	madd-4	NP_492405.3
Drosophila melanogaster (fruit fly)	CG31619	NP_001163040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007792	XP_317715.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nolo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nolo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15145	NM_165397.3 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21690	NM_001299197.1 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15144	NM_001042909.3 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa15144	NM_001259199.2 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa15146	NM_165396.3 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa15819	NM_001169569.3 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21691	NM_001299198.1 Latest version!	Drosophila melanogaster no long nerve cord (nolo), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nolo ( NM_165397.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724360.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15145
Clone ID Related Accession (Same CDS sequence)	NM_165397.3
Accession Version	NM_165397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165397.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT GTTGAACCGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724360.1
CDS	433..3477
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDVEPF

Target ORF information:

RefSeq Version	NM_165397.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165397.3

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT GTTGAACCGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_001299197.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286126.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa21690
Clone ID Related Accession (Same CDS sequence)	NM_001299197.1
Accession Version	NM_001299197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GTGTCGTCCT 
CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG GCCATGGGCT 
TGTGTCGACT TCATCGCTAC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286126.1
CDS	626..4252
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLSQLVQSEDRIPQPASASSGGSRTRTLLAMPYFQALLSNLQLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQCRPRQPSYVGIPHVIAVMPGPWACVDFIATS

Target ORF information:

RefSeq Version	NM_001299197.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299197.1

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GTGTCGTCCT 
CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG GCCATGGGCT 
TGTGTCGACT TCATCGCTAC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_001259199.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246128.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant E, mRNA.

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CloneID	OFa15144
Clone ID Related Accession (Same CDS sequence)	NM_001259199.2 , NM_001042909.3
Accession Version	NM_001259199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259199.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG 
GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC 
AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG 
AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA 
TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC 
AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC 
TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG 
TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC 
CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT 
CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT 
TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC 
TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246128.1
CDS	626..4810
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLSQLVQSEDRIPQPASASSGGSRTRTLLAMPYFQALLSNLQLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEACPQWSKGDWTPCQRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYNERCEGVWRVEPWSECNAPCGRKGIKYRILQCVWYGSRRPAGNVCKHQPRPAVMKVCKSPACQAQLSSSPAQLCRDSSRYCRNARAMGLCRLHRYKLKCCGSCEQQPNQHLIL

Target ORF information:

RefSeq Version	NM_001259199.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259199.2

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG 
GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC 
AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG 
AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA 
TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC 
AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC 
TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG 
TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC 
CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT 
CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT 
TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC 
TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_001042909.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036374.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant C, mRNA.

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CloneID	OFa15144
Clone ID Related Accession (Same CDS sequence)	NM_001259199.2 , NM_001042909.3
Accession Version	NM_001042909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001042909.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG 
GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC 
AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG 
AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA 
TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC 
AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC 
TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG 
TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC 
CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT 
CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT 
TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC 
TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036374.1
CDS	433..4617
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLSQLVQSEDRIPQPASASSGGSRTRTLLAMPYFQALLSNLQLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEACPQWSKGDWTPCQRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYNERCEGVWRVEPWSECNAPCGRKGIKYRILQCVWYGSRRPAGNVCKHQPRPAVMKVCKSPACQAQLSSSPAQLCRDSSRYCRNARAMGLCRLHRYKLKCCGSCEQQPNQHLIL

Target ORF information:

RefSeq Version	NM_001042909.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042909.3

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA 
GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC 
GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA 
GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG 
GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT 
TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC 
GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG 
GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC 
AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG 
AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA 
TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC 
AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC 
TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG 
TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC 
CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT 
CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT 
TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC 
TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_165396.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724359.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15146
Clone ID Related Accession (Same CDS sequence)	NM_165396.3
Accession Version	NM_165396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165396.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT TTGCTATGGC CGCTGCAGCG CTTTAAAGAC 
TCTCGTGGTC AGCACTTGCT TCAAGGCGAG GCGCTTAAGT TCGGCATTGA TTTAAGCGGC 
AAAGACTTTG ACCAGGACGA GTCGGAGCGT GTTCCGGCAG AACAGCTTCC CCCAGAAGTA 
TTGCATTCGC CATCGGACCC AGAGTACCAT GAGCCGTCGA AGTCCGGTCC GGAAGCGGAT 
GTTCCGCATG TTCCGCCACA CATGCCACAC GCTCGATGGG AAACGGGAGC TGCAGCTTCG 
ACTACGGCGG TGCCAAGCCT TCCGAACGAC GGTTTCGTCT ACAGATGGAT GTTGGGCGAA 
TGGTCCCAGT GTTCGCAGGA GTGTGGAGCA GCCGGCAGTG GGTTGCAGCG GCGCACGGTG 
AGCTGCCAGA GAGCTGCAAA AGCCACGAGC TCCGGCAGTA CTAGCGACAC CGAAAACAAT 
GTCGTGGATA ATTCAGAATG CTTCGCGCAC GGCCTGGAGC TGCCCGAATT TTTCCAGAGC 
TGCGGCAACG AGGCATGCCC GCAGTGGAGC AAGGGCGACT GGACTCCCTG CCAGCGATCC 
CACTGCCACG GAAGGAATAC GGCCATGCAG CGCCGCGAAG TGACGTGCCG CTACCCCAAC 
GGTACGGAAG GAAGCATCTG CGACGAGTAC GAGCGACCTG CGATGCGACA GGAATGCTAC 
AATGAGCGAT GCGAGGGAGT GTGGCGCGTG GAGCCTTGGT CCGAGTGCAA TGCGCCGTGC 
GGCCGTAAGG GCATCAAGTA CCGCATCCTG CAGTGCGTCT GGTACGGAAG TCGCCGCCCA 
GCCGGCAATG TGTGCAAGCA CCAGCCACGC CCGGCGGTTA TGAAGGTCTG CAAAAGTCCG 
GCATGCCAGG CGCAGCTGTC GTCCTCGCCA GCCCAGCTAT GTCGGGATTC CTCACGTTAT 
TGCCGTAATG CCAGGGCCAT GGGCTTGTGT CGACTTCATC GCTACAAGCT GAAGTGCTGC 
GGGTCCTGCG AGCAGCAACC TAATCAACAT CTTATTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724359.1
CDS	626..4687
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEACPQWSKGDWTPCQRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYNERCEGVWRVEPWSECNAPCGRKGIKYRILQCVWYGSRRPAGNVCKHQPRPAVMKVCKSPACQAQLSSSPAQLCRDSSRYCRNARAMGLCRLHRYKLKCCGSCEQQPNQHLIL

Target ORF information:

RefSeq Version	NM_165396.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165396.3

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT TTGCTATGGC CGCTGCAGCG CTTTAAAGAC 
TCTCGTGGTC AGCACTTGCT TCAAGGCGAG GCGCTTAAGT TCGGCATTGA TTTAAGCGGC 
AAAGACTTTG ACCAGGACGA GTCGGAGCGT GTTCCGGCAG AACAGCTTCC CCCAGAAGTA 
TTGCATTCGC CATCGGACCC AGAGTACCAT GAGCCGTCGA AGTCCGGTCC GGAAGCGGAT 
GTTCCGCATG TTCCGCCACA CATGCCACAC GCTCGATGGG AAACGGGAGC TGCAGCTTCG 
ACTACGGCGG TGCCAAGCCT TCCGAACGAC GGTTTCGTCT ACAGATGGAT GTTGGGCGAA 
TGGTCCCAGT GTTCGCAGGA GTGTGGAGCA GCCGGCAGTG GGTTGCAGCG GCGCACGGTG 
AGCTGCCAGA GAGCTGCAAA AGCCACGAGC TCCGGCAGTA CTAGCGACAC CGAAAACAAT 
GTCGTGGATA ATTCAGAATG CTTCGCGCAC GGCCTGGAGC TGCCCGAATT TTTCCAGAGC 
TGCGGCAACG AGGCATGCCC GCAGTGGAGC AAGGGCGACT GGACTCCCTG CCAGCGATCC 
CACTGCCACG GAAGGAATAC GGCCATGCAG CGCCGCGAAG TGACGTGCCG CTACCCCAAC 
GGTACGGAAG GAAGCATCTG CGACGAGTAC GAGCGACCTG CGATGCGACA GGAATGCTAC 
AATGAGCGAT GCGAGGGAGT GTGGCGCGTG GAGCCTTGGT CCGAGTGCAA TGCGCCGTGC 
GGCCGTAAGG GCATCAAGTA CCGCATCCTG CAGTGCGTCT GGTACGGAAG TCGCCGCCCA 
GCCGGCAATG TGTGCAAGCA CCAGCCACGC CCGGCGGTTA TGAAGGTCTG CAAAAGTCCG 
GCATGCCAGG CGCAGCTGTC GTCCTCGCCA GCCCAGCTAT GTCGGGATTC CTCACGTTAT 
TGCCGTAATG CCAGGGCCAT GGGCTTGTGT CGACTTCATC GCTACAAGCT GAAGTGCTGC 
GGGTCCTGCG AGCAGCAACC TAATCAACAT CTTATTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_001169569.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163040.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant D, mRNA.

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CloneID	OFa15819
Clone ID Related Accession (Same CDS sequence)	NM_001169569.3
Accession Version	NM_001169569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169569.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGCGCTTTA AAGACTCTCG TGGTCAGCAC 
TTGCTTCAAG GCGAGGCGCT TAAGTTCGGC ATTGATTTAA GCGGCAAAGA CTTTGACCAG 
GACGAGTCGG AGCGTGTTCC GGCAGAACAG CTTCCCCCAG AAGTATTGCA TTCGCCATCG 
GACCCAGAGT ACCATGAGCC GTCGAAGTCC GGTCCGGAAG CGGATGTTCC GCATGTTCCG 
CCACACATGC CACACGCTCG ATGGGAAACG GGAGCTGCAG CTTCGACTAC GGCGGTGCCA 
AGCCTTCCGA ACGACGGTTT CGTCTACAGA TGGATGTTGG GCGAATGGTC CCAGTGTTCG 
CAGGAGTGTG GAGCAGCCGG CAGTGGGTTG CAGCGGCGCA CGGTGAGCTG CCAGAGAGCT 
GCAAAAGCCA CGAGCTCCGG CAGTACTAGC GACACCGAAA ACAATGTCGT GGATAATTCA 
GAATGCTTCG CGCACGGCCT GGAGCTGCCC GAATTTTTCC AGAGCTGCGG CAACGAGGCA 
TGCCCGCAGT GGAGCAAGGG CGACTGGACT CCCTGCCAGC GATCCCACTG CCACGGAAGG 
AATACGGCCA TGCAGCGCCG CGAAGTGACG TGCCGCTACC CCAACGGTAC GGAAGGAAGC 
ATCTGCGACG AGTACGAGCG ACCTGCGATG CGACAGGAAT GCTACAATGA GCGATGCGAG 
GGAGTGTGGC GCGTGGAGCC TTGGTCCGAG TGCAATGCGC CGTGCGGCCG TAAGGGCATC 
AAGTACCGCA TCCTGCAGTG CGTCTGGTAC GGAAGTCGCC GCCCAGCCGG CAATGTGTGC 
AAGCACCAGC CACGCCCGGC GGTTATGAAG GTCTGCAAAA GTCCGGCATG CCAGGCGCAG 
CTGTCGTCCT CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG 
GCCATGGGCT TGTGTCGACT TCATCGCTAC AAGCTGAAGT GCTGCGGGTC CTGCGAGCAG 
CAACCTAATC AACATCTTAT TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163040.1
CDS	433..4599
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLSQLVQSEDRIPQPASASSGGSRTRTLLAMPYFQALLSNLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEACPQWSKGDWTPCQRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYNERCEGVWRVEPWSECNAPCGRKGIKYRILQCVWYGSRRPAGNVCKHQPRPAVMKVCKSPACQAQLSSSPAQLCRDSSRYCRNARAMGLCRLHRYKLKCCGSCEQQPNQHLIL

Target ORF information:

RefSeq Version	NM_001169569.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169569.3

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC 
GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC 
ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC 
AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC 
AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA 
GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG 
AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT 
TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC 
TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG 
CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT 
TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC 
ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG 
TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC 
TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG 
GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC 
GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT 
GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC 
AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC 
TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT 
ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG 
CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC 
TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA 
ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA 
CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC 
AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG 
AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC 
CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG 
ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG 
GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA 
CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG 
TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG 
CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA 
ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG 
CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGCGCTTTA AAGACTCTCG TGGTCAGCAC 
TTGCTTCAAG GCGAGGCGCT TAAGTTCGGC ATTGATTTAA GCGGCAAAGA CTTTGACCAG 
GACGAGTCGG AGCGTGTTCC GGCAGAACAG CTTCCCCCAG AAGTATTGCA TTCGCCATCG 
GACCCAGAGT ACCATGAGCC GTCGAAGTCC GGTCCGGAAG CGGATGTTCC GCATGTTCCG 
CCACACATGC CACACGCTCG ATGGGAAACG GGAGCTGCAG CTTCGACTAC GGCGGTGCCA 
AGCCTTCCGA ACGACGGTTT CGTCTACAGA TGGATGTTGG GCGAATGGTC CCAGTGTTCG 
CAGGAGTGTG GAGCAGCCGG CAGTGGGTTG CAGCGGCGCA CGGTGAGCTG CCAGAGAGCT 
GCAAAAGCCA CGAGCTCCGG CAGTACTAGC GACACCGAAA ACAATGTCGT GGATAATTCA 
GAATGCTTCG CGCACGGCCT GGAGCTGCCC GAATTTTTCC AGAGCTGCGG CAACGAGGCA 
TGCCCGCAGT GGAGCAAGGG CGACTGGACT CCCTGCCAGC GATCCCACTG CCACGGAAGG 
AATACGGCCA TGCAGCGCCG CGAAGTGACG TGCCGCTACC CCAACGGTAC GGAAGGAAGC 
ATCTGCGACG AGTACGAGCG ACCTGCGATG CGACAGGAAT GCTACAATGA GCGATGCGAG 
GGAGTGTGGC GCGTGGAGCC TTGGTCCGAG TGCAATGCGC CGTGCGGCCG TAAGGGCATC 
AAGTACCGCA TCCTGCAGTG CGTCTGGTAC GGAAGTCGCC GCCCAGCCGG CAATGTGTGC 
AAGCACCAGC CACGCCCGGC GGTTATGAAG GTCTGCAAAA GTCCGGCATG CCAGGCGCAG 
CTGTCGTCCT CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG 
GCCATGGGCT TGTGTCGACT TCATCGCTAC AAGCTGAAGT GCTGCGGGTC CTGCGAGCAG 
CAACCTAATC AACATCTTAT TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolo ( NM_001299198.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286127.1 Drosophila melanogaster no long nerve cord (nolo), transcript variant G, mRNA.

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CloneID	OFa21691
Clone ID Related Accession (Same CDS sequence)	NM_001299198.1
Accession Version	NM_001299198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no long nerve cord, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
GGTATACAAA TGCGAGGCGT CGAGTGCAAA TCGACAGACG GCAGCCTGAG CGCGAAGTGT 
GATCCACTTA CAAAGCCGGG CAGCATGCAG CAGTGCTCCA CCGGAATTCA TTGCGGCGGA 
TCGTTGAACA AAGTTGGTGG CACCATCATC GTAGGCAGCA GCCGGTCACT TAACGAACGA 
TCGGAGCGAC AGTTGGACAG CTCGGATGCA GATGAAGACA ACGAGGACGA AAATGATGAG 
GGCGATGATG TGGACGACCT GGAATCGGGA CAGGATACAG ATGATGGCGA AGGATTGTCA 
TATGCTGATC AACCTTTGCT TTACGCTCAT CGCACACAGA GTCGACTGAA TCAGGAAGCC 
CCAGATGAGC CGCGCACCAT GCATCTAATG AACGGAAATT CAAACAATAA TTTCAATCGG 
GGAGAGGACG AGTCAGAGGG ACCATCACTT GATCCCACCT ACATCAAGGA CAATGAGTGG 
TCCCCATGTA GCGTTACCTG TGGGGAAGGA ATCCGCCGGC GCACCTACAA CTGCAAGATT 
TTCCTTGAAT ACTCTCGAAC GGTGGCCACC GTTAATGATT CGTTGTGCGA GGGAAAAAAG 
CCACATGACG AAGTAGAGCG GTGTGTGGAG GATCCGTGCA TGCTACCCTC CCACGGCTTT 
GACGACCAGT TCCCCCGTGA TTCCATTAAA GTGGGTGTGT CGGAACCGGG TAAGACGTAT 
GTCTGGCGGG AACAGGGATA CACCTCCTGC AGTGCCTCCT GTCTCGGCGG TGTCGAGGAA 
CTGATAATCA ACTGCGTTAG AGAAGATAAT GGTAGGGTGG TGTCGCCCTT TCTCTGTTCC 
CCGGAAACCA AGCCGGAGGC CAGGGTTCGT ACCTGCAACG ACCGTCCCTG CCCACCCAGA 
TGGAACTACT CAGACTACAC ACCCTGCTCC AAGAGCTGTG GCATTGGCAT CAAGACCCGC 
GAGGTGCAGT GCATCCACGA GGTTACGCGC GGAGGGGACA ACACAATGGT GGTGCCGAAC 
AGTATGTGCC CACAGCCACC ACCAGCAGAT CGGCAGTACT GCAACGTTCT CGATTGCCCC 
GTACGTTGGG AAGTCGGTGA GTGGAGCAAG TGCTCCCATA CGTGTGGCTA TGGGTTTAAG 
GATCGCAAGG TGGAGTGCAA GCAGATAATG GCGCAAGAGC ACAAGATCGA ACGACCCGAA 
TCGATGTGTC CGAGTGCTAA GCCGGCGGAC AAAAAGCCCT GCAATGTGAA GCCCTGCCCG 
CCGGAAGATC CCAAGCCAGT CATCCAGATC AACAACTCAA CACACATCCA GCACGACCCC 
AAGAAGACCA AGATCACCCT TAAAGTGGGC GGCGCTGGAC TTGTTTTCTT CGGCACCCAG 
GTAAAGATCA AGTGTCCCGT GAAGCGGTAC AACCGCACCA AGATCAAGTG GAGCAAGGAT 
CACAAGCCGC TGCAGCGTTC GCGAAAGTAC AAAGTGTCGA AGAAGGGAGC CCTGCGTATA 
CTTGATATTA CGTTTCGCGA TGCAGGGGTG TACTCCTGCC ACGCCGGTCT CAGCTCCGCA 
GAAATAAGCA TCGAAGTGAA GGCCAAGCCA GGACAGCAGA TTGAGGAGCT GGAACAACAG 
GAAACTGAAC GATTGGTGCG TGAGAAAAGT GGCACAGAGG CTCTCACCTC TGCGGATATG 
AAGTCCGCTG ATGGAACTGG GACTTCCACT GCGGTGGGAC CTAATAGCCA CGTTAACGGT 
TCGGCACAAC AGCATGGTTC GCGTCGCAGG CAGCAGCAGT CGGAGCGATT GCAGAACGGG 
AGGGAACGGA GCCGGAGACC CAAGTCCGAT GGAGTTCAGC ATGCGGATAG CAGCATTATG 
GAAGACGATT TGCTATGGCC GCTGCAGCGC TTTAAAGACT CTCGTGGTCA GCACTTGCTT 
CAAGGCGAGG CGCTTAAGTT CGGCATTGAT TTAAGCGGCA AAGACTTTGA CCAGGACGAG 
TCGGAGCGTG TTCCGGCAGA ACAGCTTCCC CCAGAAGTAT TGCATTCGCC ATCGGACCCA 
GAGTACCATG AGCCGTCGAA GTCCGGTCCG GAAGCGGATG TTCCGCATGT TCCGCCACAC 
ATGCCACACG CTCGATGGGA AACGGGAGCT GCAGCTTCGA CTACGGCGGT GCCAAGCCTT 
CCGAACGACG GTTTCGTCTA CAGATGGATG TTGGGCGAAT GGTCCCAGTG TTCGCAGGAG 
TGTGGAGCAG CCGGCAGTGG GTTGCAGCGG CGCACGGTGA GCTGCCAGAG AGCTGCAAAA 
GCCACGAGCT CCGGCAGTAC TAGCGACACC GAAAACAATG TCGTGGATAA TTCAGAATGC 
TTCGCGCACG GCCTGGAGCT GCCCGAATTT TTCCAGAGCT GCGGCAACGA GGCATGCCCG 
CAGTGGAGCA AGGGCGACTG GACTCCCTGC CAGCGATCCC ACTGCCACGG AAGGAATACG 
GCCATGCAGC GCCGCGAAGT GACGTGCCGC TACCCCAACG GTACGGAAGG AAGCATCTGC 
GACGAGTACG AGCGACCTGC GATGCGACAG GAATGCTACA ATGAGCGATG CGAGGGAGTG 
TGGCGCGTGG AGCCTTGGTC CGAGTGCAAT GCGCCGTGCG GCCGTAAGGG CATCAAGTAC 
CGCATCCTGC AGTGCGTCTG GTACGGAAGT CGCCGCCCAG CCGGCAATGT GTGCAAGCAC 
CAGCCACGCC CGGCGGTTAT GAAGGTCTGC AAAAGTCCGG CATGCCAGGC GCAGCTGTCG 
TCCTCGCCAG CCCAGCTATG TCGGGATTCC TCACGTTATT GCCGTAATGC CAGGGCCATG 
GGCTTGTGTC GACTTCATCG CTACAAGCTG AAGTGCTGCG GGTCCTGCGA GCAGCAACCT 
AATCAACATC TTATTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286127.1
CDS	433..4473
Translation	MSVNMNWTSPKIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGPGQWSSWSDWSTCSRTCDGGIMHQMRRCGSPGSCRGESTRYRICNMQPCPEQQDFRSSQCSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQLSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQCSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPPRWIADDWSTCSRLCGHGYRERMVVCAEESNGIKTRVADIMCRTPKPPTQETCIIEECPHWEVEDWTGGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSEPGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPCPPRWNYSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQPPPADRQYCNVLDCPVRWEVGEWSKCSHTCGYGFKDRKVECKQIMAQEHKIERPESMCPSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKTKITLKVGGAGLVFFGTQVKIKCPVKRYNRTKIKWSKDHKPLQRSRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSGTEALTSADMKSADGTGTSTAVGPNSHVNGSAQQHGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLPPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEWSQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEACPQWSKGDWTPCQRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYNERCEGVWRVEPWSECNAPCGRKGIKYRILQCVWYGSRRPAGNVCKHQPRPAVMKVCKSPACQAQLSSSPAQLCRDSSRYCRNARAMGLCRLHRYKLKCCGSCEQQPNQHLIL

Target ORF information:

RefSeq Version	NM_001299198.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no long nerve cord (nolo), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299198.1

ORF Insert Sequence:

ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT 
GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG 
AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC 
TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT 
AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG 
CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT 
CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG 
CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC 
GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG 
AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG 
TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA 
GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG 
ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG 
AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG 
GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG 
AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG 
GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA 
ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT 
GGTATACAAA TGCGAGGCGT CGAGTGCAAA TCGACAGACG GCAGCCTGAG CGCGAAGTGT 
GATCCACTTA CAAAGCCGGG CAGCATGCAG CAGTGCTCCA CCGGAATTCA TTGCGGCGGA 
TCGTTGAACA AAGTTGGTGG CACCATCATC GTAGGCAGCA GCCGGTCACT TAACGAACGA 
TCGGAGCGAC AGTTGGACAG CTCGGATGCA GATGAAGACA ACGAGGACGA AAATGATGAG 
GGCGATGATG TGGACGACCT GGAATCGGGA CAGGATACAG ATGATGGCGA AGGATTGTCA 
TATGCTGATC AACCTTTGCT TTACGCTCAT CGCACACAGA GTCGACTGAA TCAGGAAGCC 
CCAGATGAGC CGCGCACCAT GCATCTAATG AACGGAAATT CAAACAATAA TTTCAATCGG 
GGAGAGGACG AGTCAGAGGG ACCATCACTT GATCCCACCT ACATCAAGGA CAATGAGTGG 
TCCCCATGTA GCGTTACCTG TGGGGAAGGA ATCCGCCGGC GCACCTACAA CTGCAAGATT 
TTCCTTGAAT ACTCTCGAAC GGTGGCCACC GTTAATGATT CGTTGTGCGA GGGAAAAAAG 
CCACATGACG AAGTAGAGCG GTGTGTGGAG GATCCGTGCA TGCTACCCTC CCACGGCTTT 
GACGACCAGT TCCCCCGTGA TTCCATTAAA GTGGGTGTGT CGGAACCGGG TAAGACGTAT 
GTCTGGCGGG AACAGGGATA CACCTCCTGC AGTGCCTCCT GTCTCGGCGG TGTCGAGGAA 
CTGATAATCA ACTGCGTTAG AGAAGATAAT GGTAGGGTGG TGTCGCCCTT TCTCTGTTCC 
CCGGAAACCA AGCCGGAGGC CAGGGTTCGT ACCTGCAACG ACCGTCCCTG CCCACCCAGA 
TGGAACTACT CAGACTACAC ACCCTGCTCC AAGAGCTGTG GCATTGGCAT CAAGACCCGC 
GAGGTGCAGT GCATCCACGA GGTTACGCGC GGAGGGGACA ACACAATGGT GGTGCCGAAC 
AGTATGTGCC CACAGCCACC ACCAGCAGAT CGGCAGTACT GCAACGTTCT CGATTGCCCC 
GTACGTTGGG AAGTCGGTGA GTGGAGCAAG TGCTCCCATA CGTGTGGCTA TGGGTTTAAG 
GATCGCAAGG TGGAGTGCAA GCAGATAATG GCGCAAGAGC ACAAGATCGA ACGACCCGAA 
TCGATGTGTC CGAGTGCTAA GCCGGCGGAC AAAAAGCCCT GCAATGTGAA GCCCTGCCCG 
CCGGAAGATC CCAAGCCAGT CATCCAGATC AACAACTCAA CACACATCCA GCACGACCCC 
AAGAAGACCA AGATCACCCT TAAAGTGGGC GGCGCTGGAC TTGTTTTCTT CGGCACCCAG 
GTAAAGATCA AGTGTCCCGT GAAGCGGTAC AACCGCACCA AGATCAAGTG GAGCAAGGAT 
CACAAGCCGC TGCAGCGTTC GCGAAAGTAC AAAGTGTCGA AGAAGGGAGC CCTGCGTATA 
CTTGATATTA CGTTTCGCGA TGCAGGGGTG TACTCCTGCC ACGCCGGTCT CAGCTCCGCA 
GAAATAAGCA TCGAAGTGAA GGCCAAGCCA GGACAGCAGA TTGAGGAGCT GGAACAACAG 
GAAACTGAAC GATTGGTGCG TGAGAAAAGT GGCACAGAGG CTCTCACCTC TGCGGATATG 
AAGTCCGCTG ATGGAACTGG GACTTCCACT GCGGTGGGAC CTAATAGCCA CGTTAACGGT 
TCGGCACAAC AGCATGGTTC GCGTCGCAGG CAGCAGCAGT CGGAGCGATT GCAGAACGGG 
AGGGAACGGA GCCGGAGACC CAAGTCCGAT GGAGTTCAGC ATGCGGATAG CAGCATTATG 
GAAGACGATT TGCTATGGCC GCTGCAGCGC TTTAAAGACT CTCGTGGTCA GCACTTGCTT 
CAAGGCGAGG CGCTTAAGTT CGGCATTGAT TTAAGCGGCA AAGACTTTGA CCAGGACGAG 
TCGGAGCGTG TTCCGGCAGA ACAGCTTCCC CCAGAAGTAT TGCATTCGCC ATCGGACCCA 
GAGTACCATG AGCCGTCGAA GTCCGGTCCG GAAGCGGATG TTCCGCATGT TCCGCCACAC 
ATGCCACACG CTCGATGGGA AACGGGAGCT GCAGCTTCGA CTACGGCGGT GCCAAGCCTT 
CCGAACGACG GTTTCGTCTA CAGATGGATG TTGGGCGAAT GGTCCCAGTG TTCGCAGGAG 
TGTGGAGCAG CCGGCAGTGG GTTGCAGCGG CGCACGGTGA GCTGCCAGAG AGCTGCAAAA 
GCCACGAGCT CCGGCAGTAC TAGCGACACC GAAAACAATG TCGTGGATAA TTCAGAATGC 
TTCGCGCACG GCCTGGAGCT GCCCGAATTT TTCCAGAGCT GCGGCAACGA GGCATGCCCG 
CAGTGGAGCA AGGGCGACTG GACTCCCTGC CAGCGATCCC ACTGCCACGG AAGGAATACG 
GCCATGCAGC GCCGCGAAGT GACGTGCCGC TACCCCAACG GTACGGAAGG AAGCATCTGC 
GACGAGTACG AGCGACCTGC GATGCGACAG GAATGCTACA ATGAGCGATG CGAGGGAGTG 
TGGCGCGTGG AGCCTTGGTC CGAGTGCAAT GCGCCGTGCG GCCGTAAGGG CATCAAGTAC 
CGCATCCTGC AGTGCGTCTG GTACGGAAGT CGCCGCCCAG CCGGCAATGT GTGCAAGCAC 
CAGCCACGCC CGGCGGTTAT GAAGGTCTGC AAAAGTCCGG CATGCCAGGC GCAGCTGTCG 
TCCTCGCCAG CCCAGCTATG TCGGGATTCC TCACGTTATT GCCGTAATGC CAGGGCCATG 
GGCTTGTGTC GACTTCATCG CTACAAGCTG AAGTGCTGCG GGTCCTGCGA GCAGCAACCT 
AATCAACATC TTATTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nolo cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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