nolo cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following nolo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nolo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa15145 NM_165397.3
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa21690 NM_001299197.1
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa15144 NM_001042909.3
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa15144 NM_001259199.2
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa15146 NM_165396.3
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa15819 NM_001169569.3
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa21691 NM_001299198.1
Latest version!
Drosophila melanogaster no long nerve cord (nolo), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa15145
    Clone ID Related Accession (Same CDS sequence) NM_165397.3
    Accession Version NM_165397.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3045bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165397.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT GTTGAACCGT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724360.1
    CDS433..3477
    Translation

    Target ORF information:

    RefSeq Version NM_165397.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165397.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT GTTGAACCGT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21690
    Clone ID Related Accession (Same CDS sequence) NM_001299197.1
    Accession Version NM_001299197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GTGTCGTCCT
    CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG GCCATGGGCT
    TGTGTCGACT TCATCGCTAC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286126.1
    CDS626..4252
    Translation

    Target ORF information:

    RefSeq Version NM_001299197.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GTGTCGTCCT
    CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG GCCATGGGCT
    TGTGTCGACT TCATCGCTAC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15144
    Clone ID Related Accession (Same CDS sequence) NM_001259199.2 , NM_001042909.3
    Accession Version NM_001259199.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4185bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259199.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG
    GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC
    AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG
    AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA
    TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC
    AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC
    TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG
    TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC
    CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT
    CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT
    TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC
    TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246128.1
    CDS626..4810
    Translation

    Target ORF information:

    RefSeq Version NM_001259199.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG
    GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC
    AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG
    AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA
    TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC
    AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC
    TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG
    TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC
    CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT
    CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT
    TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC
    TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15144
    Clone ID Related Accession (Same CDS sequence) NM_001259199.2 , NM_001042909.3
    Accession Version NM_001042909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4185bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001042909.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG
    GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC
    AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG
    AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA
    TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC
    AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC
    TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG
    TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC
    CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT
    CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT
    TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC
    TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036374.1
    CDS433..4617
    Translation

    Target ORF information:

    RefSeq Version NM_001042909.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGTTGCTAT GGCCGCTGCA GCGCTTTAAA
    GACTCTCGTG GTCAGCACTT GCTTCAAGGC GAGGCGCTTA AGTTCGGCAT TGATTTAAGC
    GGCAAAGACT TTGACCAGGA CGAGTCGGAG CGTGTTCCGG CAGAACAGCT TCCCCCAGAA
    GTATTGCATT CGCCATCGGA CCCAGAGTAC CATGAGCCGT CGAAGTCCGG TCCGGAAGCG
    GATGTTCCGC ATGTTCCGCC ACACATGCCA CACGCTCGAT GGGAAACGGG AGCTGCAGCT
    TCGACTACGG CGGTGCCAAG CCTTCCGAAC GACGGTTTCG TCTACAGATG GATGTTGGGC
    GAATGGTCCC AGTGTTCGCA GGAGTGTGGA GCAGCCGGCA GTGGGTTGCA GCGGCGCACG
    GTGAGCTGCC AGAGAGCTGC AAAAGCCACG AGCTCCGGCA GTACTAGCGA CACCGAAAAC
    AATGTCGTGG ATAATTCAGA ATGCTTCGCG CACGGCCTGG AGCTGCCCGA ATTTTTCCAG
    AGCTGCGGCA ACGAGGCATG CCCGCAGTGG AGCAAGGGCG ACTGGACTCC CTGCCAGCGA
    TCCCACTGCC ACGGAAGGAA TACGGCCATG CAGCGCCGCG AAGTGACGTG CCGCTACCCC
    AACGGTACGG AAGGAAGCAT CTGCGACGAG TACGAGCGAC CTGCGATGCG ACAGGAATGC
    TACAATGAGC GATGCGAGGG AGTGTGGCGC GTGGAGCCTT GGTCCGAGTG CAATGCGCCG
    TGCGGCCGTA AGGGCATCAA GTACCGCATC CTGCAGTGCG TCTGGTACGG AAGTCGCCGC
    CCAGCCGGCA ATGTGTGCAA GCACCAGCCA CGCCCGGCGG TTATGAAGGT CTGCAAAAGT
    CCGGCATGCC AGGCGCAGCT GTCGTCCTCG CCAGCCCAGC TATGTCGGGA TTCCTCACGT
    TATTGCCGTA ATGCCAGGGC CATGGGCTTG TGTCGACTTC ATCGCTACAA GCTGAAGTGC
    TGCGGGTCCT GCGAGCAGCA ACCTAATCAA CATCTTATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15146
    Clone ID Related Accession (Same CDS sequence) NM_165396.3
    Accession Version NM_165396.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165396.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT TTGCTATGGC CGCTGCAGCG CTTTAAAGAC
    TCTCGTGGTC AGCACTTGCT TCAAGGCGAG GCGCTTAAGT TCGGCATTGA TTTAAGCGGC
    AAAGACTTTG ACCAGGACGA GTCGGAGCGT GTTCCGGCAG AACAGCTTCC CCCAGAAGTA
    TTGCATTCGC CATCGGACCC AGAGTACCAT GAGCCGTCGA AGTCCGGTCC GGAAGCGGAT
    GTTCCGCATG TTCCGCCACA CATGCCACAC GCTCGATGGG AAACGGGAGC TGCAGCTTCG
    ACTACGGCGG TGCCAAGCCT TCCGAACGAC GGTTTCGTCT ACAGATGGAT GTTGGGCGAA
    TGGTCCCAGT GTTCGCAGGA GTGTGGAGCA GCCGGCAGTG GGTTGCAGCG GCGCACGGTG
    AGCTGCCAGA GAGCTGCAAA AGCCACGAGC TCCGGCAGTA CTAGCGACAC CGAAAACAAT
    GTCGTGGATA ATTCAGAATG CTTCGCGCAC GGCCTGGAGC TGCCCGAATT TTTCCAGAGC
    TGCGGCAACG AGGCATGCCC GCAGTGGAGC AAGGGCGACT GGACTCCCTG CCAGCGATCC
    CACTGCCACG GAAGGAATAC GGCCATGCAG CGCCGCGAAG TGACGTGCCG CTACCCCAAC
    GGTACGGAAG GAAGCATCTG CGACGAGTAC GAGCGACCTG CGATGCGACA GGAATGCTAC
    AATGAGCGAT GCGAGGGAGT GTGGCGCGTG GAGCCTTGGT CCGAGTGCAA TGCGCCGTGC
    GGCCGTAAGG GCATCAAGTA CCGCATCCTG CAGTGCGTCT GGTACGGAAG TCGCCGCCCA
    GCCGGCAATG TGTGCAAGCA CCAGCCACGC CCGGCGGTTA TGAAGGTCTG CAAAAGTCCG
    GCATGCCAGG CGCAGCTGTC GTCCTCGCCA GCCCAGCTAT GTCGGGATTC CTCACGTTAT
    TGCCGTAATG CCAGGGCCAT GGGCTTGTGT CGACTTCATC GCTACAAGCT GAAGTGCTGC
    GGGTCCTGCG AGCAGCAACC TAATCAACAT CTTATTCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724359.1
    CDS626..4687
    Translation

    Target ORF information:

    RefSeq Version NM_165396.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165396.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT TTGCTATGGC CGCTGCAGCG CTTTAAAGAC
    TCTCGTGGTC AGCACTTGCT TCAAGGCGAG GCGCTTAAGT TCGGCATTGA TTTAAGCGGC
    AAAGACTTTG ACCAGGACGA GTCGGAGCGT GTTCCGGCAG AACAGCTTCC CCCAGAAGTA
    TTGCATTCGC CATCGGACCC AGAGTACCAT GAGCCGTCGA AGTCCGGTCC GGAAGCGGAT
    GTTCCGCATG TTCCGCCACA CATGCCACAC GCTCGATGGG AAACGGGAGC TGCAGCTTCG
    ACTACGGCGG TGCCAAGCCT TCCGAACGAC GGTTTCGTCT ACAGATGGAT GTTGGGCGAA
    TGGTCCCAGT GTTCGCAGGA GTGTGGAGCA GCCGGCAGTG GGTTGCAGCG GCGCACGGTG
    AGCTGCCAGA GAGCTGCAAA AGCCACGAGC TCCGGCAGTA CTAGCGACAC CGAAAACAAT
    GTCGTGGATA ATTCAGAATG CTTCGCGCAC GGCCTGGAGC TGCCCGAATT TTTCCAGAGC
    TGCGGCAACG AGGCATGCCC GCAGTGGAGC AAGGGCGACT GGACTCCCTG CCAGCGATCC
    CACTGCCACG GAAGGAATAC GGCCATGCAG CGCCGCGAAG TGACGTGCCG CTACCCCAAC
    GGTACGGAAG GAAGCATCTG CGACGAGTAC GAGCGACCTG CGATGCGACA GGAATGCTAC
    AATGAGCGAT GCGAGGGAGT GTGGCGCGTG GAGCCTTGGT CCGAGTGCAA TGCGCCGTGC
    GGCCGTAAGG GCATCAAGTA CCGCATCCTG CAGTGCGTCT GGTACGGAAG TCGCCGCCCA
    GCCGGCAATG TGTGCAAGCA CCAGCCACGC CCGGCGGTTA TGAAGGTCTG CAAAAGTCCG
    GCATGCCAGG CGCAGCTGTC GTCCTCGCCA GCCCAGCTAT GTCGGGATTC CTCACGTTAT
    TGCCGTAATG CCAGGGCCAT GGGCTTGTGT CGACTTCATC GCTACAAGCT GAAGTGCTGC
    GGGTCCTGCG AGCAGCAACC TAATCAACAT CTTATTCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15819
    Clone ID Related Accession (Same CDS sequence) NM_001169569.3
    Accession Version NM_001169569.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169569.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGCGCTTTA AAGACTCTCG TGGTCAGCAC
    TTGCTTCAAG GCGAGGCGCT TAAGTTCGGC ATTGATTTAA GCGGCAAAGA CTTTGACCAG
    GACGAGTCGG AGCGTGTTCC GGCAGAACAG CTTCCCCCAG AAGTATTGCA TTCGCCATCG
    GACCCAGAGT ACCATGAGCC GTCGAAGTCC GGTCCGGAAG CGGATGTTCC GCATGTTCCG
    CCACACATGC CACACGCTCG ATGGGAAACG GGAGCTGCAG CTTCGACTAC GGCGGTGCCA
    AGCCTTCCGA ACGACGGTTT CGTCTACAGA TGGATGTTGG GCGAATGGTC CCAGTGTTCG
    CAGGAGTGTG GAGCAGCCGG CAGTGGGTTG CAGCGGCGCA CGGTGAGCTG CCAGAGAGCT
    GCAAAAGCCA CGAGCTCCGG CAGTACTAGC GACACCGAAA ACAATGTCGT GGATAATTCA
    GAATGCTTCG CGCACGGCCT GGAGCTGCCC GAATTTTTCC AGAGCTGCGG CAACGAGGCA
    TGCCCGCAGT GGAGCAAGGG CGACTGGACT CCCTGCCAGC GATCCCACTG CCACGGAAGG
    AATACGGCCA TGCAGCGCCG CGAAGTGACG TGCCGCTACC CCAACGGTAC GGAAGGAAGC
    ATCTGCGACG AGTACGAGCG ACCTGCGATG CGACAGGAAT GCTACAATGA GCGATGCGAG
    GGAGTGTGGC GCGTGGAGCC TTGGTCCGAG TGCAATGCGC CGTGCGGCCG TAAGGGCATC
    AAGTACCGCA TCCTGCAGTG CGTCTGGTAC GGAAGTCGCC GCCCAGCCGG CAATGTGTGC
    AAGCACCAGC CACGCCCGGC GGTTATGAAG GTCTGCAAAA GTCCGGCATG CCAGGCGCAG
    CTGTCGTCCT CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG
    GCCATGGGCT TGTGTCGACT TCATCGCTAC AAGCTGAAGT GCTGCGGGTC CTGCGAGCAG
    CAACCTAATC AACATCTTAT TCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163040.1
    CDS433..4599
    Translation

    Target ORF information:

    RefSeq Version NM_001169569.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169569.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    TGTTCGGTTT CCTGCGGACA GGGTATACAA ATGCGAGGCG TCGAGTGCAA ATCGACAGAC
    GGCAGCCTGA GCGCGAAGTG TGATCCACTT ACAAAGCCGG GCAGCATGCA GCAGTGCTCC
    ACCGGAATTC ATTGCGGCGG ATCGTTGAAC AAAGTTGGTG GCACCATCAT CGTAGGCAGC
    AGCCGGTCAC TTAACGAACG ATCGGAGCGA CAGTTGGACA GCTCGGATGC AGATGAAGAC
    AACGAGGACG AAAATGATGA GGGCGATGAT GTGGACGACC TGGAATCGGG ACAGGATACA
    GATGATGGCG AAGGATTGTC ATATGCTGAT CAACCTTTGC TTTACGCTCA TCGCACACAG
    AGTCGACTGA ATCAGGAAGC CCCAGATGAG CCGCGCACCA TGCATCTAAT GAACGGAAAT
    TCAAACAATA ATTTCAATCG GGGAGAGGAC GAGTCAGAGG GACCATCACT TGATCCCACC
    TACATCAAGG ACAATGAGTG GTCCCCATGT AGCGTTACCT GTGGGGAAGG AATCCGCCGG
    CGCACCTACA ACTGCAAGAT TTTCCTTGAA TACTCTCGAA CGGTGGCCAC CGTTAATGAT
    TCGTTGTGCG AGGGAAAAAA GCCACATGAC GAAGTAGAGC GGTGTGTGGA GGATCCGTGC
    ATGCTACCCT CCCACGGCTT TGACGACCAG TTCCCCCGTG ATTCCATTAA AGTGGGTGTG
    TCGGAACCGG GTAAGACGTA TGTCTGGCGG GAACAGGGAT ACACCTCCTG CAGTGCCTCC
    TGTCTCGGCG GTGTCGAGGA ACTGATAATC AACTGCGTTA GAGAAGATAA TGGTAGGGTG
    GTGTCGCCCT TTCTCTGTTC CCCGGAAACC AAGCCGGAGG CCAGGGTTCG TACCTGCAAC
    GACCGTCCCT GCCCACCCAG ATGGAACTAC TCAGACTACA CACCCTGCTC CAAGAGCTGT
    GGCATTGGCA TCAAGACCCG CGAGGTGCAG TGCATCCACG AGGTTACGCG CGGAGGGGAC
    AACACAATGG TGGTGCCGAA CAGTATGTGC CCACAGCCAC CACCAGCAGA TCGGCAGTAC
    TGCAACGTTC TCGATTGCCC CGTACGTTGG GAAGTCGGTG AGTGGAGCAA GTGCTCCCAT
    ACGTGTGGCT ATGGGTTTAA GGATCGCAAG GTGGAGTGCA AGCAGATAAT GGCGCAAGAG
    CACAAGATCG AACGACCCGA ATCGATGTGT CCGAGTGCTA AGCCGGCGGA CAAAAAGCCC
    TGCAATGTGA AGCCCTGCCC GCCGGAAGAT CCCAAGCCAG TCATCCAGAT CAACAACTCA
    ACACACATCC AGCACGACCC CAAGAAGACC AAGATCACCC TTAAAGTGGG CGGCGCTGGA
    CTTGTTTTCT TCGGCACCCA GGTAAAGATC AAGTGTCCCG TGAAGCGGTA CAACCGCACC
    AAGATCAAGT GGAGCAAGGA TCACAAGCCG CTGCAGCGTT CGCGAAAGTA CAAAGTGTCG
    AAGAAGGGAG CCCTGCGTAT ACTTGATATT ACGTTTCGCG ATGCAGGGGT GTACTCCTGC
    CACGCCGGTC TCAGCTCCGC AGAAATAAGC ATCGAAGTGA AGGCCAAGCC AGGACAGCAG
    ATTGAGGAGC TGGAACAACA GGAAACTGAA CGATTGGTGC GTGAGAAAAG TGGCACAGAG
    GCTCTCACCT CTGCGGATAT GAAGTCCGCT GATGGAACTG GGACTTCCAC TGCGGTGGGA
    CCTAATAGCC ACGTTAACGG TTCGGCACAA CAGCATGGTT CGCGTCGCAG GCAGCAGCAG
    TCGGAGCGAT TGCAGAACGG GAGGGAACGG AGCCGGAGAC CCAAGTCCGA TGGAGTTCAG
    CATGCGGATA GCAGCATTAT GGAAGACGAT CTTTCGCAAT TAGTGCAATC CGAGGACAGA
    ATACCACAGC CGGCGAGTGC CAGTAGCGGC GGTAGTCGCA CCAGGACGCT GCTGGCTATG
    CCATATTTCC AGGCTCTGCT TAGCAATCTC CAGCGCTTTA AAGACTCTCG TGGTCAGCAC
    TTGCTTCAAG GCGAGGCGCT TAAGTTCGGC ATTGATTTAA GCGGCAAAGA CTTTGACCAG
    GACGAGTCGG AGCGTGTTCC GGCAGAACAG CTTCCCCCAG AAGTATTGCA TTCGCCATCG
    GACCCAGAGT ACCATGAGCC GTCGAAGTCC GGTCCGGAAG CGGATGTTCC GCATGTTCCG
    CCACACATGC CACACGCTCG ATGGGAAACG GGAGCTGCAG CTTCGACTAC GGCGGTGCCA
    AGCCTTCCGA ACGACGGTTT CGTCTACAGA TGGATGTTGG GCGAATGGTC CCAGTGTTCG
    CAGGAGTGTG GAGCAGCCGG CAGTGGGTTG CAGCGGCGCA CGGTGAGCTG CCAGAGAGCT
    GCAAAAGCCA CGAGCTCCGG CAGTACTAGC GACACCGAAA ACAATGTCGT GGATAATTCA
    GAATGCTTCG CGCACGGCCT GGAGCTGCCC GAATTTTTCC AGAGCTGCGG CAACGAGGCA
    TGCCCGCAGT GGAGCAAGGG CGACTGGACT CCCTGCCAGC GATCCCACTG CCACGGAAGG
    AATACGGCCA TGCAGCGCCG CGAAGTGACG TGCCGCTACC CCAACGGTAC GGAAGGAAGC
    ATCTGCGACG AGTACGAGCG ACCTGCGATG CGACAGGAAT GCTACAATGA GCGATGCGAG
    GGAGTGTGGC GCGTGGAGCC TTGGTCCGAG TGCAATGCGC CGTGCGGCCG TAAGGGCATC
    AAGTACCGCA TCCTGCAGTG CGTCTGGTAC GGAAGTCGCC GCCCAGCCGG CAATGTGTGC
    AAGCACCAGC CACGCCCGGC GGTTATGAAG GTCTGCAAAA GTCCGGCATG CCAGGCGCAG
    CTGTCGTCCT CGCCAGCCCA GCTATGTCGG GATTCCTCAC GTTATTGCCG TAATGCCAGG
    GCCATGGGCT TGTGTCGACT TCATCGCTAC AAGCTGAAGT GCTGCGGGTC CTGCGAGCAG
    CAACCTAATC AACATCTTAT TCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21691
    Clone ID Related Accession (Same CDS sequence) NM_001299198.1
    Accession Version NM_001299198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product no long nerve cord, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    GGTATACAAA TGCGAGGCGT CGAGTGCAAA TCGACAGACG GCAGCCTGAG CGCGAAGTGT
    GATCCACTTA CAAAGCCGGG CAGCATGCAG CAGTGCTCCA CCGGAATTCA TTGCGGCGGA
    TCGTTGAACA AAGTTGGTGG CACCATCATC GTAGGCAGCA GCCGGTCACT TAACGAACGA
    TCGGAGCGAC AGTTGGACAG CTCGGATGCA GATGAAGACA ACGAGGACGA AAATGATGAG
    GGCGATGATG TGGACGACCT GGAATCGGGA CAGGATACAG ATGATGGCGA AGGATTGTCA
    TATGCTGATC AACCTTTGCT TTACGCTCAT CGCACACAGA GTCGACTGAA TCAGGAAGCC
    CCAGATGAGC CGCGCACCAT GCATCTAATG AACGGAAATT CAAACAATAA TTTCAATCGG
    GGAGAGGACG AGTCAGAGGG ACCATCACTT GATCCCACCT ACATCAAGGA CAATGAGTGG
    TCCCCATGTA GCGTTACCTG TGGGGAAGGA ATCCGCCGGC GCACCTACAA CTGCAAGATT
    TTCCTTGAAT ACTCTCGAAC GGTGGCCACC GTTAATGATT CGTTGTGCGA GGGAAAAAAG
    CCACATGACG AAGTAGAGCG GTGTGTGGAG GATCCGTGCA TGCTACCCTC CCACGGCTTT
    GACGACCAGT TCCCCCGTGA TTCCATTAAA GTGGGTGTGT CGGAACCGGG TAAGACGTAT
    GTCTGGCGGG AACAGGGATA CACCTCCTGC AGTGCCTCCT GTCTCGGCGG TGTCGAGGAA
    CTGATAATCA ACTGCGTTAG AGAAGATAAT GGTAGGGTGG TGTCGCCCTT TCTCTGTTCC
    CCGGAAACCA AGCCGGAGGC CAGGGTTCGT ACCTGCAACG ACCGTCCCTG CCCACCCAGA
    TGGAACTACT CAGACTACAC ACCCTGCTCC AAGAGCTGTG GCATTGGCAT CAAGACCCGC
    GAGGTGCAGT GCATCCACGA GGTTACGCGC GGAGGGGACA ACACAATGGT GGTGCCGAAC
    AGTATGTGCC CACAGCCACC ACCAGCAGAT CGGCAGTACT GCAACGTTCT CGATTGCCCC
    GTACGTTGGG AAGTCGGTGA GTGGAGCAAG TGCTCCCATA CGTGTGGCTA TGGGTTTAAG
    GATCGCAAGG TGGAGTGCAA GCAGATAATG GCGCAAGAGC ACAAGATCGA ACGACCCGAA
    TCGATGTGTC CGAGTGCTAA GCCGGCGGAC AAAAAGCCCT GCAATGTGAA GCCCTGCCCG
    CCGGAAGATC CCAAGCCAGT CATCCAGATC AACAACTCAA CACACATCCA GCACGACCCC
    AAGAAGACCA AGATCACCCT TAAAGTGGGC GGCGCTGGAC TTGTTTTCTT CGGCACCCAG
    GTAAAGATCA AGTGTCCCGT GAAGCGGTAC AACCGCACCA AGATCAAGTG GAGCAAGGAT
    CACAAGCCGC TGCAGCGTTC GCGAAAGTAC AAAGTGTCGA AGAAGGGAGC CCTGCGTATA
    CTTGATATTA CGTTTCGCGA TGCAGGGGTG TACTCCTGCC ACGCCGGTCT CAGCTCCGCA
    GAAATAAGCA TCGAAGTGAA GGCCAAGCCA GGACAGCAGA TTGAGGAGCT GGAACAACAG
    GAAACTGAAC GATTGGTGCG TGAGAAAAGT GGCACAGAGG CTCTCACCTC TGCGGATATG
    AAGTCCGCTG ATGGAACTGG GACTTCCACT GCGGTGGGAC CTAATAGCCA CGTTAACGGT
    TCGGCACAAC AGCATGGTTC GCGTCGCAGG CAGCAGCAGT CGGAGCGATT GCAGAACGGG
    AGGGAACGGA GCCGGAGACC CAAGTCCGAT GGAGTTCAGC ATGCGGATAG CAGCATTATG
    GAAGACGATT TGCTATGGCC GCTGCAGCGC TTTAAAGACT CTCGTGGTCA GCACTTGCTT
    CAAGGCGAGG CGCTTAAGTT CGGCATTGAT TTAAGCGGCA AAGACTTTGA CCAGGACGAG
    TCGGAGCGTG TTCCGGCAGA ACAGCTTCCC CCAGAAGTAT TGCATTCGCC ATCGGACCCA
    GAGTACCATG AGCCGTCGAA GTCCGGTCCG GAAGCGGATG TTCCGCATGT TCCGCCACAC
    ATGCCACACG CTCGATGGGA AACGGGAGCT GCAGCTTCGA CTACGGCGGT GCCAAGCCTT
    CCGAACGACG GTTTCGTCTA CAGATGGATG TTGGGCGAAT GGTCCCAGTG TTCGCAGGAG
    TGTGGAGCAG CCGGCAGTGG GTTGCAGCGG CGCACGGTGA GCTGCCAGAG AGCTGCAAAA
    GCCACGAGCT CCGGCAGTAC TAGCGACACC GAAAACAATG TCGTGGATAA TTCAGAATGC
    TTCGCGCACG GCCTGGAGCT GCCCGAATTT TTCCAGAGCT GCGGCAACGA GGCATGCCCG
    CAGTGGAGCA AGGGCGACTG GACTCCCTGC CAGCGATCCC ACTGCCACGG AAGGAATACG
    GCCATGCAGC GCCGCGAAGT GACGTGCCGC TACCCCAACG GTACGGAAGG AAGCATCTGC
    GACGAGTACG AGCGACCTGC GATGCGACAG GAATGCTACA ATGAGCGATG CGAGGGAGTG
    TGGCGCGTGG AGCCTTGGTC CGAGTGCAAT GCGCCGTGCG GCCGTAAGGG CATCAAGTAC
    CGCATCCTGC AGTGCGTCTG GTACGGAAGT CGCCGCCCAG CCGGCAATGT GTGCAAGCAC
    CAGCCACGCC CGGCGGTTAT GAAGGTCTGC AAAAGTCCGG CATGCCAGGC GCAGCTGTCG
    TCCTCGCCAG CCCAGCTATG TCGGGATTCC TCACGTTATT GCCGTAATGC CAGGGCCATG
    GGCTTGTGTC GACTTCATCG CTACAAGCTG AAGTGCTGCG GGTCCTGCGA GCAGCAACCT
    AATCAACATC TTATTCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286127.1
    CDS433..4473
    Translation

    Target ORF information:

    RefSeq Version NM_001299198.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster no long nerve cord (nolo), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGTGA ACATGAACTG GACGTCTCCA AAAATATGGG CCGCTTTGTT TGTGACCACC 
    CTATGCCTGT TGGATACACA GGCCGCCTGG GCAAAAAAGC TAAATGCAAG TCAAGCTTTT
    GACGACACGT GGAACACAGC ATCCGATTTG GAAAATGAAC TGGAACAACA AAAGCGATCG
    AAGGTCCAAA GTGCAGGAGG AGGAGGTGGC GCGGGCGGTG GACCTGGCCA GTGGTCGTCC
    TGGTCAGATT GGTCAACCTG TTCGCGCACC TGCGACGGAG GCATCATGCA TCAAATGCGT
    AGGTGCGGCA GTCCTGGCAG TTGTCGCGGG GAGAGCACCC GCTACCGCAT TTGCAACATG
    CAGCCTTGTC CGGAGCAGCA GGACTTCCGC TCCAGCCAAT GCTCCGCCTA CAACGATGTT
    CCATACGATG GCACCCTGTA CAAGTGGACT CCGCATTATG ATTACGTCGA GCCCTGCGCG
    CTTACATGCA GAGGACATCC TGCACATCTT GTCGAAGACA TTTCGCGGGA GACTGGAGAC
    GGCAATGCAG AGGAGGCGGA GCACTACGAC GAGCAGAGCG TCATTGTGCA ATTGTCTGCG
    AGGGTGCAAG ACGGAACCCG TTGTCGTTCC GGCAGCCTAG ATATGTGTAT CCAAGGAAAG
    TGTCAGCGTG TTGGCTGCGA TTTAAAAATC GGATCTACAA AAAAAATCGA CGGTTGCGGA
    GTGTGCGGCG GAGATGGAAA CTCCTGCTCC CAACCGCTTT TTAACTGGGA GATGGCACCG
    ATGTCGCAGT GCTCTGTAAC CTGTGGAAGC GGTTACAAAA TGTCCCGTCC AATATGCAGG
    AATCGCCTAA CCAACGCCGA TGTCGATGAT ACGCTCTGCA GCGTTACTAA TCGTCCGGAG
    GCGTCGGTGG AACAATGTAA TACACACAGT TGTCCTCCCC GGTGGATAGC AGACGATTGG
    AGCACTTGCA GCCGCCTTTG TGGTCACGGC TACCGTGAGC GGATGGTTGT CTGTGCCGAG
    GAGTCGAACG GCATCAAGAC GAGGGTGGCC GATATCATGT GCCGCACACC CAAGCCACCA
    ACACAAGAGA CCTGCATTAT CGAGGAGTGT CCGCACTGGG AGGTCGAAGA CTGGACCGGT
    GGTATACAAA TGCGAGGCGT CGAGTGCAAA TCGACAGACG GCAGCCTGAG CGCGAAGTGT
    GATCCACTTA CAAAGCCGGG CAGCATGCAG CAGTGCTCCA CCGGAATTCA TTGCGGCGGA
    TCGTTGAACA AAGTTGGTGG CACCATCATC GTAGGCAGCA GCCGGTCACT TAACGAACGA
    TCGGAGCGAC AGTTGGACAG CTCGGATGCA GATGAAGACA ACGAGGACGA AAATGATGAG
    GGCGATGATG TGGACGACCT GGAATCGGGA CAGGATACAG ATGATGGCGA AGGATTGTCA
    TATGCTGATC AACCTTTGCT TTACGCTCAT CGCACACAGA GTCGACTGAA TCAGGAAGCC
    CCAGATGAGC CGCGCACCAT GCATCTAATG AACGGAAATT CAAACAATAA TTTCAATCGG
    GGAGAGGACG AGTCAGAGGG ACCATCACTT GATCCCACCT ACATCAAGGA CAATGAGTGG
    TCCCCATGTA GCGTTACCTG TGGGGAAGGA ATCCGCCGGC GCACCTACAA CTGCAAGATT
    TTCCTTGAAT ACTCTCGAAC GGTGGCCACC GTTAATGATT CGTTGTGCGA GGGAAAAAAG
    CCACATGACG AAGTAGAGCG GTGTGTGGAG GATCCGTGCA TGCTACCCTC CCACGGCTTT
    GACGACCAGT TCCCCCGTGA TTCCATTAAA GTGGGTGTGT CGGAACCGGG TAAGACGTAT
    GTCTGGCGGG AACAGGGATA CACCTCCTGC AGTGCCTCCT GTCTCGGCGG TGTCGAGGAA
    CTGATAATCA ACTGCGTTAG AGAAGATAAT GGTAGGGTGG TGTCGCCCTT TCTCTGTTCC
    CCGGAAACCA AGCCGGAGGC CAGGGTTCGT ACCTGCAACG ACCGTCCCTG CCCACCCAGA
    TGGAACTACT CAGACTACAC ACCCTGCTCC AAGAGCTGTG GCATTGGCAT CAAGACCCGC
    GAGGTGCAGT GCATCCACGA GGTTACGCGC GGAGGGGACA ACACAATGGT GGTGCCGAAC
    AGTATGTGCC CACAGCCACC ACCAGCAGAT CGGCAGTACT GCAACGTTCT CGATTGCCCC
    GTACGTTGGG AAGTCGGTGA GTGGAGCAAG TGCTCCCATA CGTGTGGCTA TGGGTTTAAG
    GATCGCAAGG TGGAGTGCAA GCAGATAATG GCGCAAGAGC ACAAGATCGA ACGACCCGAA
    TCGATGTGTC CGAGTGCTAA GCCGGCGGAC AAAAAGCCCT GCAATGTGAA GCCCTGCCCG
    CCGGAAGATC CCAAGCCAGT CATCCAGATC AACAACTCAA CACACATCCA GCACGACCCC
    AAGAAGACCA AGATCACCCT TAAAGTGGGC GGCGCTGGAC TTGTTTTCTT CGGCACCCAG
    GTAAAGATCA AGTGTCCCGT GAAGCGGTAC AACCGCACCA AGATCAAGTG GAGCAAGGAT
    CACAAGCCGC TGCAGCGTTC GCGAAAGTAC AAAGTGTCGA AGAAGGGAGC CCTGCGTATA
    CTTGATATTA CGTTTCGCGA TGCAGGGGTG TACTCCTGCC ACGCCGGTCT CAGCTCCGCA
    GAAATAAGCA TCGAAGTGAA GGCCAAGCCA GGACAGCAGA TTGAGGAGCT GGAACAACAG
    GAAACTGAAC GATTGGTGCG TGAGAAAAGT GGCACAGAGG CTCTCACCTC TGCGGATATG
    AAGTCCGCTG ATGGAACTGG GACTTCCACT GCGGTGGGAC CTAATAGCCA CGTTAACGGT
    TCGGCACAAC AGCATGGTTC GCGTCGCAGG CAGCAGCAGT CGGAGCGATT GCAGAACGGG
    AGGGAACGGA GCCGGAGACC CAAGTCCGAT GGAGTTCAGC ATGCGGATAG CAGCATTATG
    GAAGACGATT TGCTATGGCC GCTGCAGCGC TTTAAAGACT CTCGTGGTCA GCACTTGCTT
    CAAGGCGAGG CGCTTAAGTT CGGCATTGAT TTAAGCGGCA AAGACTTTGA CCAGGACGAG
    TCGGAGCGTG TTCCGGCAGA ACAGCTTCCC CCAGAAGTAT TGCATTCGCC ATCGGACCCA
    GAGTACCATG AGCCGTCGAA GTCCGGTCCG GAAGCGGATG TTCCGCATGT TCCGCCACAC
    ATGCCACACG CTCGATGGGA AACGGGAGCT GCAGCTTCGA CTACGGCGGT GCCAAGCCTT
    CCGAACGACG GTTTCGTCTA CAGATGGATG TTGGGCGAAT GGTCCCAGTG TTCGCAGGAG
    TGTGGAGCAG CCGGCAGTGG GTTGCAGCGG CGCACGGTGA GCTGCCAGAG AGCTGCAAAA
    GCCACGAGCT CCGGCAGTAC TAGCGACACC GAAAACAATG TCGTGGATAA TTCAGAATGC
    TTCGCGCACG GCCTGGAGCT GCCCGAATTT TTCCAGAGCT GCGGCAACGA GGCATGCCCG
    CAGTGGAGCA AGGGCGACTG GACTCCCTGC CAGCGATCCC ACTGCCACGG AAGGAATACG
    GCCATGCAGC GCCGCGAAGT GACGTGCCGC TACCCCAACG GTACGGAAGG AAGCATCTGC
    GACGAGTACG AGCGACCTGC GATGCGACAG GAATGCTACA ATGAGCGATG CGAGGGAGTG
    TGGCGCGTGG AGCCTTGGTC CGAGTGCAAT GCGCCGTGCG GCCGTAAGGG CATCAAGTAC
    CGCATCCTGC AGTGCGTCTG GTACGGAAGT CGCCGCCCAG CCGGCAATGT GTGCAAGCAC
    CAGCCACGCC CGGCGGTTAT GAAGGTCTGC AAAAGTCCGG CATGCCAGGC GCAGCTGTCG
    TCCTCGCCAG CCCAGCTATG TCGGGATTCC TCACGTTATT GCCGTAATGC CAGGGCCATG
    GGCTTGTGTC GACTTCATCG CTACAAGCTG AAGTGCTGCG GGTCCTGCGA GCAGCAACCT
    AATCAACATC TTATTCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.