ref(2)P cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ref(2)P
Entrez Gene ID	35246
Full Name	refractory to sigma P
Synonyms	CG10360,Dmel\CG10360,Ref(2)P,Ref(2)P/p62,Ref(2)p,Ref2P,SQSTM1,p62,p62/Ref(2)P,p63,ref,ref(2)Pn,ref(2)Po2,ref(2)p,ref2p
General protein information	Preferred Names refractory to sigma P Names CG10360 gene product from transcript CG10360-RB CG10360-PA CG10360-PB ref(2)P-PA ref(2)P-PB refractaire refractaire(2)Paris sequestosome sequestosome-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37F1-37F1\|2-54.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057352.4	NP_476700.1	refractory to sigma P, isoform A
NM_001014491.2	NP_001014491.1	refractory to sigma P, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Atg17-positive structures appear at aggregates of the selective cargo Ref(2)P/p62 near lysosomes
Title: Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila.

Ref(2)P is a crucial downstream effector of a pathway involving Pink1 and Parkin.
Title: Drosophila ref(2)P is required for the parkin-mediated suppression of mitochondrial dysfunction in pink1 mutants.

p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects
Title: p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects.

Our findings reveal a major role for Ref(2)P in the formation of ubiquitin-positive protein aggregates both under physiological conditions and when normal protein turnover is inhibited.
Title: Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain.

A single (albeit complex) mutation in the gene ref(2)P confers resistance against sigma and show that this mutation has increased in frequency under positive selection.
Title: The age and evolution of an antiviral resistance mutation in Drosophila melanogaster.

The following ref(2)P gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ref(2)P cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14991	NM_057352.4 Latest version!	Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14991	NM_001014491.2 Latest version!	Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ref(2)P ( NM_057352.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476700.1 Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14991
Clone ID Related Accession (Same CDS sequence)	NM_057352.4 , NM_001014491.2
Accession Version	NM_057352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	refractory to sigma P, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057352.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGGAGA AGCTGTTGAA AATCACCTAC CAGGGCGCTG GACCCCAGAA AAAGATAAAT 
GCATACCTGC GGATGCCCTC CCAGAATTAC ACCATATTGC GTCGCGAAAT CGAGCTGTAT 
CTTTTCCAGG AGCGCCAGTT ACCCAAATGC GACGTAAGGA CCTTCTGGAT CGACGCTGAT 
AAAGATGAAA TCGAAATAGT CAACCAAAAT GACTATGAGA TTTTCCTGGC CAAGTGCGAG 
AGCAATATGC ACGTTCAGGT GGCTCCACTG GCGCCCGTAG AGGAGCCAAA GGCCACCAAG 
CAAGAGGGTT CTTCGGCCAA CGCTGAAGCT CCTTCTGTCG ACGATCCGAG CAATTTCACC 
ATCCACGACG CCGTTGAATG CGATGGCTGC GGCTTGGCTC CCTTGATTGG GTTCCGCTAC 
AAGTGCGTTC AGTGCAGCAA CTATGATCTT TGCCAGAAAT GCGAGTTGGC TCACAAGCAT 
CCTGAGCACT TGATGCTACG CATGCCGACC AACAATGGAC CCGGTATGGT CGATGCCTGG 
TTCACAGGTC CAGGATTGGG ACGCCGCAGT GGCCGGCGCT CCAGGGGACA TTGCCCGTTT 
CAGGAGACGA ACCAGGCAGA TCCTGCTGGC GAACCCGCAA GGGATAGTCG CCGTGAACGG 
CGTCAGGCCC GCCGCCATGC CGGAGTCTTG ACTCAGTTTG TCGAGATGAT GACCAACTTG 
CCGCTGAACA CAACAACGGC CACGGCACCA GCCGAACCGC AGAAACCGAA GGCTGCAGAA 
CAAACTGAAA GTCCTCCACA AGCTGAGCCC ACTGTTACCG CTGAAAAGGC AGCTGAATCC 
GAGGCCAAAC CAACTGAGCC GAAGAAGGTT AACACTGATC AAAGTGTCCC TAGAACCGAA 
GATCCAGTGA CGACTCCACG TTCCACTCAG CCAACTACTC CAGTGATTAA TCTGGATAAC 
ATTTCGCAGA TTGTGCCACC CGAGTACATG AGTGCTGGCA TCGAAATCCT TAATAACTTC 
AGCGAAATGT TTTCCAAGAT TATCGATACC ACTGAGGGCG GTGATTCGGG AATTTTTGCG 
CCCTCAACGA CTCCCAGTGC TGAGAATAAG AAACCAGAAG AGCAGGGTCA ATCCAGTGGC 
CAGTCGGGGG CATCCTCGGC CAACCAGTCA GCTGTTCCCT CTGCCGCTCC ATCGGCTAAT 
CAGTCTAATG TCCCATCTGC TAACCAGTCG GCCACTCCAT CAATTTCTGG TTCGATCCCT 
GATGCTCAGC TTGAGACAGA GCCCCTGAAT CCTAAGCCAT CGGAAACCAC CACCGAAACA 
GAGCAGGAAA GACGCCGTTC AGACAGCTTG GATCCAGAGT GGCAGCTTAT TGACAATGCA 
TACTCTGCAA ACAATAGTAA CTTGATCAAT CTGGACACAA CCAATCCCAC TGCTGCTCCT 
CAGGAGCCGG TGCGTGATTT TGGTCAGCTG GGCGAACTAT TGCGTCAGCA CATGAATGAG 
GAGGCTCGCG TGGAGCAGGC TTCGGCCAAC ACCCAGACTG CTCAAGTTGA TACGGTGAGT 
ACATCGACCT CGACCACATC GGTGACCACC AATAGCGTCG GCACCTCCCC AGCAGCTCCC 
GACGACAAGC GCACGGTCCC CGTCTACCAC ACTGATGAAA GCATCAATAA ATCGATCCAT 
GCCATGATGG CCATGGGTTT CAGCAACGAG GGCGCCTGGC TAACCCAGCT CCTAGAGTCG 
GTTCAGGGCA ATATCTCAGC TGCCTTGGAC GTAATGAACG TATCGCAGAA CCGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476700.1
CDS	350..2149
Translation	MPEKLLKITYQGAGPQKKINAYLRMPSQNYTILRREIELYLFQERQLPKCDVRTFWIDADKDEIEIVNQNDYEIFLAKCESNMHVQVAPLAPVEEPKATKQEGSSANAEAPSVDDPSNFTIHDAVECDGCGLAPLIGFRYKCVQCSNYDLCQKCELAHKHPEHLMLRMPTNNGPGMVDAWFTGPGLGRRSGRRSRGHCPFQETNQADPAGEPARDSRRERRQARRHAGVLTQFVEMMTNLPLNTTTATAPAEPQKPKAAEQTESPPQAEPTVTAEKAAESEAKPTEPKKVNTDQSVPRTEDPVTTPRSTQPTTPVINLDNISQIVPPEYMSAGIEILNNFSEMFSKIIDTTEGGDSGIFAPSTTPSAENKKPEEQGQSSGQSGASSANQSAVPSAAPSANQSNVPSANQSATPSISGSIPDAQLETEPLNPKPSETTTETEQERRRSDSLDPEWQLIDNAYSANNSNLINLDTTNPTAAPQEPVRDFGQLGELLRQHMNEEARVEQASANTQTAQVDTVSTSTSTTSVTTNSVGTSPAAPDDKRTVPVYHTDESINKSIHAMMAMGFSNEGAWLTQLLESVQGNISAALDVMNVSQNRN

Target ORF information:

RefSeq Version	NM_057352.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057352.4

ORF Insert Sequence:

ATGCCGGAGA AGCTGTTGAA AATCACCTAC CAGGGCGCTG GACCCCAGAA AAAGATAAAT 
GCATACCTGC GGATGCCCTC CCAGAATTAC ACCATATTGC GTCGCGAAAT CGAGCTGTAT 
CTTTTCCAGG AGCGCCAGTT ACCCAAATGC GACGTAAGGA CCTTCTGGAT CGACGCTGAT 
AAAGATGAAA TCGAAATAGT CAACCAAAAT GACTATGAGA TTTTCCTGGC CAAGTGCGAG 
AGCAATATGC ACGTTCAGGT GGCTCCACTG GCGCCCGTAG AGGAGCCAAA GGCCACCAAG 
CAAGAGGGTT CTTCGGCCAA CGCTGAAGCT CCTTCTGTCG ACGATCCGAG CAATTTCACC 
ATCCACGACG CCGTTGAATG CGATGGCTGC GGCTTGGCTC CCTTGATTGG GTTCCGCTAC 
AAGTGCGTTC AGTGCAGCAA CTATGATCTT TGCCAGAAAT GCGAGTTGGC TCACAAGCAT 
CCTGAGCACT TGATGCTACG CATGCCGACC AACAATGGAC CCGGTATGGT CGATGCCTGG 
TTCACAGGTC CAGGATTGGG ACGCCGCAGT GGCCGGCGCT CCAGGGGACA TTGCCCGTTT 
CAGGAGACGA ACCAGGCAGA TCCTGCTGGC GAACCCGCAA GGGATAGTCG CCGTGAACGG 
CGTCAGGCCC GCCGCCATGC CGGAGTCTTG ACTCAGTTTG TCGAGATGAT GACCAACTTG 
CCGCTGAACA CAACAACGGC CACGGCACCA GCCGAACCGC AGAAACCGAA GGCTGCAGAA 
CAAACTGAAA GTCCTCCACA AGCTGAGCCC ACTGTTACCG CTGAAAAGGC AGCTGAATCC 
GAGGCCAAAC CAACTGAGCC GAAGAAGGTT AACACTGATC AAAGTGTCCC TAGAACCGAA 
GATCCAGTGA CGACTCCACG TTCCACTCAG CCAACTACTC CAGTGATTAA TCTGGATAAC 
ATTTCGCAGA TTGTGCCACC CGAGTACATG AGTGCTGGCA TCGAAATCCT TAATAACTTC 
AGCGAAATGT TTTCCAAGAT TATCGATACC ACTGAGGGCG GTGATTCGGG AATTTTTGCG 
CCCTCAACGA CTCCCAGTGC TGAGAATAAG AAACCAGAAG AGCAGGGTCA ATCCAGTGGC 
CAGTCGGGGG CATCCTCGGC CAACCAGTCA GCTGTTCCCT CTGCCGCTCC ATCGGCTAAT 
CAGTCTAATG TCCCATCTGC TAACCAGTCG GCCACTCCAT CAATTTCTGG TTCGATCCCT 
GATGCTCAGC TTGAGACAGA GCCCCTGAAT CCTAAGCCAT CGGAAACCAC CACCGAAACA 
GAGCAGGAAA GACGCCGTTC AGACAGCTTG GATCCAGAGT GGCAGCTTAT TGACAATGCA 
TACTCTGCAA ACAATAGTAA CTTGATCAAT CTGGACACAA CCAATCCCAC TGCTGCTCCT 
CAGGAGCCGG TGCGTGATTT TGGTCAGCTG GGCGAACTAT TGCGTCAGCA CATGAATGAG 
GAGGCTCGCG TGGAGCAGGC TTCGGCCAAC ACCCAGACTG CTCAAGTTGA TACGGTGAGT 
ACATCGACCT CGACCACATC GGTGACCACC AATAGCGTCG GCACCTCCCC AGCAGCTCCC 
GACGACAAGC GCACGGTCCC CGTCTACCAC ACTGATGAAA GCATCAATAA ATCGATCCAT 
GCCATGATGG CCATGGGTTT CAGCAACGAG GGCGCCTGGC TAACCCAGCT CCTAGAGTCG 
GTTCAGGGCA ATATCTCAGC TGCCTTGGAC GTAATGAACG TATCGCAGAA CCGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ref(2)P ( NM_001014491.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014491.1 Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14991
Clone ID Related Accession (Same CDS sequence)	NM_057352.4 , NM_001014491.2
Accession Version	NM_001014491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	refractory to sigma P, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001014491.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGGAGA AGCTGTTGAA AATCACCTAC CAGGGCGCTG GACCCCAGAA AAAGATAAAT 
GCATACCTGC GGATGCCCTC CCAGAATTAC ACCATATTGC GTCGCGAAAT CGAGCTGTAT 
CTTTTCCAGG AGCGCCAGTT ACCCAAATGC GACGTAAGGA CCTTCTGGAT CGACGCTGAT 
AAAGATGAAA TCGAAATAGT CAACCAAAAT GACTATGAGA TTTTCCTGGC CAAGTGCGAG 
AGCAATATGC ACGTTCAGGT GGCTCCACTG GCGCCCGTAG AGGAGCCAAA GGCCACCAAG 
CAAGAGGGTT CTTCGGCCAA CGCTGAAGCT CCTTCTGTCG ACGATCCGAG CAATTTCACC 
ATCCACGACG CCGTTGAATG CGATGGCTGC GGCTTGGCTC CCTTGATTGG GTTCCGCTAC 
AAGTGCGTTC AGTGCAGCAA CTATGATCTT TGCCAGAAAT GCGAGTTGGC TCACAAGCAT 
CCTGAGCACT TGATGCTACG CATGCCGACC AACAATGGAC CCGGTATGGT CGATGCCTGG 
TTCACAGGTC CAGGATTGGG ACGCCGCAGT GGCCGGCGCT CCAGGGGACA TTGCCCGTTT 
CAGGAGACGA ACCAGGCAGA TCCTGCTGGC GAACCCGCAA GGGATAGTCG CCGTGAACGG 
CGTCAGGCCC GCCGCCATGC CGGAGTCTTG ACTCAGTTTG TCGAGATGAT GACCAACTTG 
CCGCTGAACA CAACAACGGC CACGGCACCA GCCGAACCGC AGAAACCGAA GGCTGCAGAA 
CAAACTGAAA GTCCTCCACA AGCTGAGCCC ACTGTTACCG CTGAAAAGGC AGCTGAATCC 
GAGGCCAAAC CAACTGAGCC GAAGAAGGTT AACACTGATC AAAGTGTCCC TAGAACCGAA 
GATCCAGTGA CGACTCCACG TTCCACTCAG CCAACTACTC CAGTGATTAA TCTGGATAAC 
ATTTCGCAGA TTGTGCCACC CGAGTACATG AGTGCTGGCA TCGAAATCCT TAATAACTTC 
AGCGAAATGT TTTCCAAGAT TATCGATACC ACTGAGGGCG GTGATTCGGG AATTTTTGCG 
CCCTCAACGA CTCCCAGTGC TGAGAATAAG AAACCAGAAG AGCAGGGTCA ATCCAGTGGC 
CAGTCGGGGG CATCCTCGGC CAACCAGTCA GCTGTTCCCT CTGCCGCTCC ATCGGCTAAT 
CAGTCTAATG TCCCATCTGC TAACCAGTCG GCCACTCCAT CAATTTCTGG TTCGATCCCT 
GATGCTCAGC TTGAGACAGA GCCCCTGAAT CCTAAGCCAT CGGAAACCAC CACCGAAACA 
GAGCAGGAAA GACGCCGTTC AGACAGCTTG GATCCAGAGT GGCAGCTTAT TGACAATGCA 
TACTCTGCAA ACAATAGTAA CTTGATCAAT CTGGACACAA CCAATCCCAC TGCTGCTCCT 
CAGGAGCCGG TGCGTGATTT TGGTCAGCTG GGCGAACTAT TGCGTCAGCA CATGAATGAG 
GAGGCTCGCG TGGAGCAGGC TTCGGCCAAC ACCCAGACTG CTCAAGTTGA TACGGTGAGT 
ACATCGACCT CGACCACATC GGTGACCACC AATAGCGTCG GCACCTCCCC AGCAGCTCCC 
GACGACAAGC GCACGGTCCC CGTCTACCAC ACTGATGAAA GCATCAATAA ATCGATCCAT 
GCCATGATGG CCATGGGTTT CAGCAACGAG GGCGCCTGGC TAACCCAGCT CCTAGAGTCG 
GTTCAGGGCA ATATCTCAGC TGCCTTGGAC GTAATGAACG TATCGCAGAA CCGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014491.1
CDS	350..2149
Translation	MPEKLLKITYQGAGPQKKINAYLRMPSQNYTILRREIELYLFQERQLPKCDVRTFWIDADKDEIEIVNQNDYEIFLAKCESNMHVQVAPLAPVEEPKATKQEGSSANAEAPSVDDPSNFTIHDAVECDGCGLAPLIGFRYKCVQCSNYDLCQKCELAHKHPEHLMLRMPTNNGPGMVDAWFTGPGLGRRSGRRSRGHCPFQETNQADPAGEPARDSRRERRQARRHAGVLTQFVEMMTNLPLNTTTATAPAEPQKPKAAEQTESPPQAEPTVTAEKAAESEAKPTEPKKVNTDQSVPRTEDPVTTPRSTQPTTPVINLDNISQIVPPEYMSAGIEILNNFSEMFSKIIDTTEGGDSGIFAPSTTPSAENKKPEEQGQSSGQSGASSANQSAVPSAAPSANQSNVPSANQSATPSISGSIPDAQLETEPLNPKPSETTTETEQERRRSDSLDPEWQLIDNAYSANNSNLINLDTTNPTAAPQEPVRDFGQLGELLRQHMNEEARVEQASANTQTAQVDTVSTSTSTTSVTTNSVGTSPAAPDDKRTVPVYHTDESINKSIHAMMAMGFSNEGAWLTQLLESVQGNISAALDVMNVSQNRN

Target ORF information:

RefSeq Version	NM_001014491.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster refractory to sigma P (ref(2)P), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014491.2

ORF Insert Sequence:

ATGCCGGAGA AGCTGTTGAA AATCACCTAC CAGGGCGCTG GACCCCAGAA AAAGATAAAT 
GCATACCTGC GGATGCCCTC CCAGAATTAC ACCATATTGC GTCGCGAAAT CGAGCTGTAT 
CTTTTCCAGG AGCGCCAGTT ACCCAAATGC GACGTAAGGA CCTTCTGGAT CGACGCTGAT 
AAAGATGAAA TCGAAATAGT CAACCAAAAT GACTATGAGA TTTTCCTGGC CAAGTGCGAG 
AGCAATATGC ACGTTCAGGT GGCTCCACTG GCGCCCGTAG AGGAGCCAAA GGCCACCAAG 
CAAGAGGGTT CTTCGGCCAA CGCTGAAGCT CCTTCTGTCG ACGATCCGAG CAATTTCACC 
ATCCACGACG CCGTTGAATG CGATGGCTGC GGCTTGGCTC CCTTGATTGG GTTCCGCTAC 
AAGTGCGTTC AGTGCAGCAA CTATGATCTT TGCCAGAAAT GCGAGTTGGC TCACAAGCAT 
CCTGAGCACT TGATGCTACG CATGCCGACC AACAATGGAC CCGGTATGGT CGATGCCTGG 
TTCACAGGTC CAGGATTGGG ACGCCGCAGT GGCCGGCGCT CCAGGGGACA TTGCCCGTTT 
CAGGAGACGA ACCAGGCAGA TCCTGCTGGC GAACCCGCAA GGGATAGTCG CCGTGAACGG 
CGTCAGGCCC GCCGCCATGC CGGAGTCTTG ACTCAGTTTG TCGAGATGAT GACCAACTTG 
CCGCTGAACA CAACAACGGC CACGGCACCA GCCGAACCGC AGAAACCGAA GGCTGCAGAA 
CAAACTGAAA GTCCTCCACA AGCTGAGCCC ACTGTTACCG CTGAAAAGGC AGCTGAATCC 
GAGGCCAAAC CAACTGAGCC GAAGAAGGTT AACACTGATC AAAGTGTCCC TAGAACCGAA 
GATCCAGTGA CGACTCCACG TTCCACTCAG CCAACTACTC CAGTGATTAA TCTGGATAAC 
ATTTCGCAGA TTGTGCCACC CGAGTACATG AGTGCTGGCA TCGAAATCCT TAATAACTTC 
AGCGAAATGT TTTCCAAGAT TATCGATACC ACTGAGGGCG GTGATTCGGG AATTTTTGCG 
CCCTCAACGA CTCCCAGTGC TGAGAATAAG AAACCAGAAG AGCAGGGTCA ATCCAGTGGC 
CAGTCGGGGG CATCCTCGGC CAACCAGTCA GCTGTTCCCT CTGCCGCTCC ATCGGCTAAT 
CAGTCTAATG TCCCATCTGC TAACCAGTCG GCCACTCCAT CAATTTCTGG TTCGATCCCT 
GATGCTCAGC TTGAGACAGA GCCCCTGAAT CCTAAGCCAT CGGAAACCAC CACCGAAACA 
GAGCAGGAAA GACGCCGTTC AGACAGCTTG GATCCAGAGT GGCAGCTTAT TGACAATGCA 
TACTCTGCAA ACAATAGTAA CTTGATCAAT CTGGACACAA CCAATCCCAC TGCTGCTCCT 
CAGGAGCCGG TGCGTGATTT TGGTCAGCTG GGCGAACTAT TGCGTCAGCA CATGAATGAG 
GAGGCTCGCG TGGAGCAGGC TTCGGCCAAC ACCCAGACTG CTCAAGTTGA TACGGTGAGT 
ACATCGACCT CGACCACATC GGTGACCACC AATAGCGTCG GCACCTCCCC AGCAGCTCCC 
GACGACAAGC GCACGGTCCC CGTCTACCAC ACTGATGAAA GCATCAATAA ATCGATCCAT 
GCCATGATGG CCATGGGTTT CAGCAACGAG GGCGCCTGGC TAACCCAGCT CCTAGAGTCG 
GTTCAGGGCA ATATCTCAGC TGCCTTGGAC GTAATGAACG TATCGCAGAA CCGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ref(2)P cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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