tup cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tup
Entrez Gene ID	35147
Full Name	tailup
Synonyms	CG10619,Dmel\CG10619,Isl,Islet,TUP,Tup,isl,islet,l(2)37Aa,l(2)E41
General protein information	Preferred Names tailup Names CG10619 gene product from transcript CG10619-RA CG10619-PA CG10619-PB CG10619-PC dIslet islet islet 1 lethal(2)37Aa tail-up tailup/islet tup-PA tup-PB tup-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B1-37B1\|2-53.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057426.4	NP_476774.1	tailup, isoform B
NM_001299139.1	NP_001286068.1	tailup, isoform C
NM_057427.3	NP_476775.1	tailup, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	ISL1	NP_002193.2
Pan troglodytes (chimpanzee)	ISL1	XP_001150633.2
Canis lupus familiaris (dog)	LOC612954	XP_853721.2
Rattus norvegicus (Norway rat)	Isl1	NP_059035.3
Gallus gallus (chicken)	ISL1	NP_990745.1
Caenorhabditis elegans (roundworm)	lim-7	NP_491668.1
Bos taurus (cattle)	ISL1	NP_001092600.1
Drosophila melanogaster (fruit fly)	tup	NP_476774.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010209	XP_319393.4
Mus musculus (house mouse)	Isl1	NP_067434.3
Danio rerio (zebrafish)	isl1	NP_571037.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

tup interacts with Chip to regulate the generation of different cardiac cell types. Tup directly regulates eve expression.
Title: Chip/Ldb1 interacts with Tailup/islet1 to regulate cardiac gene expression in Drosophila.

Reinitiation of a transcriptional program previously employed for embryonic alary muscle development, in which the factors Org-1 and Tailup are key components, leads to the development of adult Drosophila ventral longitudinal heart muscles.
Title: Org-1-dependent lineage reprogramming generates the ventral longitudinal musculature of the Drosophila heart.

An Org-1-Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila.
Title: An Org-1-Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila.

These observations provide evidence for combinatorial activity of Isl and Lim3 in regulating ion channel gene expression
Title: The transcription factors islet and Lim3 combinatorially regulate ion channel gene expression.

Tailup is expressed in the main Drosophila cardiac outflow components, i.e., heart anchoring cells (HANC) and cardiac outflow muscles (COM) and that loss of its function and/or tissue-specific knockdowns dramatically affect cardiac outflow morphogenesis.
Title: Tailup plays multiple roles during cardiac outflow assembly in Drosophila.

The following tup gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tup cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16015	NM_057426.4 Latest version!	Drosophila melanogaster tailup (tup), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16016	NM_001299139.1 Latest version!	Drosophila melanogaster tailup (tup), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16016	NM_057427.3 Latest version!	Drosophila melanogaster tailup (tup), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tup ( NM_057426.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476774.1 Drosophila melanogaster tailup (tup), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16015
Clone ID Related Accession (Same CDS sequence)	NM_057426.4
Accession Version	NM_057426.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tailup, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057426.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAGT 
ATGCAGGGCA GTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476774.1
CDS	467..1864
Translation	MVMAEIGGHLAHQLPLHNHNHNQTGLQPSLVMNHHLDLDCHGHDVIKKQRLSHCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVRDGKTYCKRDYVRLFGTKCDKCGNSFSKNDFVMRAKTKIFHIECFRCSACARQLLPGDEFALRDAGALYCKEDHDVLEKSSQSSLTSSSVESNNNISSSNNNNTNLSNNNHSSELGSMSDSGSESGSHKSIRDKRPSGPSDGKPTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTSLSPRVIRVWFQNKRCKDKKKTIQMKLQMQQEKEGRKLGYGAMQGIPMIASSPVRHDSPLNLQGLDVQTYQPPWKALSDFALHADLDSNGAINTHTPAFQQLVNQMHGYDLNGMPILPPHPHSHPAQGPPHQHPPPPGGPHNHQNQQPNQQPGGSSLDSGITSHHHPDSTDSYVTYLESDDSMQGSP

Target ORF information:

RefSeq Version	NM_057426.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tailup (tup), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057426.4

ORF Insert Sequence:

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAGT 
ATGCAGGGCA GTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tup ( NM_001299139.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286068.1 Drosophila melanogaster tailup (tup), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16016
Clone ID Related Accession (Same CDS sequence)	NM_001299139.1 , NM_057427.3
Accession Version	NM_001299139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tailup, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAAA 
TCCAAGCTGG CGCTGACCCC GTCGTCCTCT TCCTCGGCCT CGGCGGGCAC ATCGATCTCC 
TCGCCGCCAT CGGGCGTCGG AGCAGGGGGT GGCGGTGCCG TGGGCGGTGG TTCAGGTGTC 
CTGGGCTTGG GCGTGGTGGC CAATCAGTCG GCCACCGAGC AGCTCATGCA AATGCTCCAG 
AAGGTAACGG GCTCGGCTTC CCCGGCCTCA CATGCGGTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286068.1
CDS	467..2071
Translation	MVMAEIGGHLAHQLPLHNHNHNQTGLQPSLVMNHHLDLDCHGHDVIKKQRLSHCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVRDGKTYCKRDYVRLFGTKCDKCGNSFSKNDFVMRAKTKIFHIECFRCSACARQLLPGDEFALRDAGALYCKEDHDVLEKSSQSSLTSSSVESNNNISSSNNNNTNLSNNNHSSELGSMSDSGSESGSHKSIRDKRPSGPSDGKPTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTSLSPRVIRVWFQNKRCKDKKKTIQMKLQMQQEKEGRKLGYGAMQGIPMIASSPVRHDSPLNLQGLDVQTYQPPWKALSDFALHADLDSNGAINTHTPAFQQLVNQMHGYDLNGMPILPPHPHSHPAQGPPHQHPPPPGGPHNHQNQQPNQQPGGSSLDSGITSHHHPDSTDSYVTYLESDDKSKLALTPSSSSSASAGTSISSPPSGVGAGGGGAVGGGSGVLGLGVVANQSATEQLMQMLQKVTGSASPASHAVL

Target ORF information:

RefSeq Version	NM_001299139.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tailup (tup), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299139.1

ORF Insert Sequence:

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAAA 
TCCAAGCTGG CGCTGACCCC GTCGTCCTCT TCCTCGGCCT CGGCGGGCAC ATCGATCTCC 
TCGCCGCCAT CGGGCGTCGG AGCAGGGGGT GGCGGTGCCG TGGGCGGTGG TTCAGGTGTC 
CTGGGCTTGG GCGTGGTGGC CAATCAGTCG GCCACCGAGC AGCTCATGCA AATGCTCCAG 
AAGGTAACGG GCTCGGCTTC CCCGGCCTCA CATGCGGTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tup ( NM_057427.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476775.1 Drosophila melanogaster tailup (tup), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16016
Clone ID Related Accession (Same CDS sequence)	NM_001299139.1 , NM_057427.3
Accession Version	NM_057427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tailup, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057427.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAAA 
TCCAAGCTGG CGCTGACCCC GTCGTCCTCT TCCTCGGCCT CGGCGGGCAC ATCGATCTCC 
TCGCCGCCAT CGGGCGTCGG AGCAGGGGGT GGCGGTGCCG TGGGCGGTGG TTCAGGTGTC 
CTGGGCTTGG GCGTGGTGGC CAATCAGTCG GCCACCGAGC AGCTCATGCA AATGCTCCAG 
AAGGTAACGG GCTCGGCTTC CCCGGCCTCA CATGCGGTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476775.1
CDS	467..2071
Translation	MVMAEIGGHLAHQLPLHNHNHNQTGLQPSLVMNHHLDLDCHGHDVIKKQRLSHCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVRDGKTYCKRDYVRLFGTKCDKCGNSFSKNDFVMRAKTKIFHIECFRCSACARQLLPGDEFALRDAGALYCKEDHDVLEKSSQSSLTSSSVESNNNISSSNNNNTNLSNNNHSSELGSMSDSGSESGSHKSIRDKRPSGPSDGKPTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTSLSPRVIRVWFQNKRCKDKKKTIQMKLQMQQEKEGRKLGYGAMQGIPMIASSPVRHDSPLNLQGLDVQTYQPPWKALSDFALHADLDSNGAINTHTPAFQQLVNQMHGYDLNGMPILPPHPHSHPAQGPPHQHPPPPGGPHNHQNQQPNQQPGGSSLDSGITSHHHPDSTDSYVTYLESDDKSKLALTPSSSSSASAGTSISSPPSGVGAGGGGAVGGGSGVLGLGVVANQSATEQLMQMLQKVTGSASPASHAVL

Target ORF information:

RefSeq Version	NM_057427.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tailup (tup), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057427.3

ORF Insert Sequence:

ATGGTAATGG CAGAGATTGG TGGCCATTTG GCTCACCAGC TACCGTTGCA CAATCACAAC 
CACAATCAAA CTGGATTACA GCCATCGCTG GTGATGAACC ATCACCTGGA TTTGGATTGC 
CATGGACACG ATGTGATAAA AAAACAACGC CTATCTCACT GCGTCGGATG TGGCGGCCAG 
ATCCACGATC AGTACATCTT GCGCGTTGCC CCCGATCTGG AGTGGCATGC GGCGTGTCTG 
AAGTGCCAGG AGTGCAGGCA GTTCCTGGAC GAAAGCTGTA CGTGTTTTGT GCGCGATGGC 
AAGACCTACT GCAAGCGTGA TTATGTTAGG TTATTTGGTA CAAAATGTGA TAAATGCGGC 
AACTCCTTCA GCAAAAATGA TTTTGTGATG CGGGCCAAAA CGAAAATCTT TCACATCGAG 
TGCTTTCGAT GCTCCGCGTG TGCGCGACAA TTGCTGCCAG GCGATGAATT CGCGTTACGC 
GATGCCGGGG CTTTGTACTG CAAGGAGGAT CACGATGTGC TGGAGAAATC GTCGCAGAGC 
AGCCTCACTT CTTCGTCGGT GGAGAGCAAC AACAATATTA GCAGTAGTAA CAACAACAAC 
ACCAACCTTA GCAATAACAA CCATTCCAGC GAATTGGGTT CAATGTCAGA TTCTGGCAGC 
GAATCGGGCT CACACAAAAG TATTAGGGAC AAGCGACCCT CGGGGCCATC GGATGGCAAG 
CCCACGCGGG TTCGGACCGT GCTCAATGAG AAACAGCTGC ATACGCTCAG AACCTGCTAC 
AATGCTAATC CGCGACCTGA TGCGCTCATG AAGGAGCAGC TGGTGGAGAT GACGAGCCTG 
TCGCCGCGAG TCATCCGAGT ATGGTTCCAG AACAAGCGCT GCAAGGACAA GAAGAAGACC 
ATTCAGATGA AGCTGCAAAT GCAGCAGGAG AAGGAGGGTC GCAAGCTGGG CTACGGCGCC 
ATGCAGGGCA TCCCCATGAT CGCCAGTTCC CCGGTGCGGC ATGACTCCCC ACTGAATCTT 
CAGGGCCTGG ATGTGCAGAC ATATCAACCG CCATGGAAAG CCTTAAGCGA CTTCGCCCTT 
CACGCCGATC TCGACAGCAA TGGAGCGATC AATACCCACA CGCCCGCATT TCAGCAGTTG 
GTCAATCAGA TGCACGGCTA CGACCTGAAC GGGATGCCCA TTCTGCCGCC GCATCCGCAC 
TCGCATCCGG CGCAAGGACC TCCCCACCAG CACCCCCCGC CCCCCGGCGG ACCGCACAAC 
CATCAGAACC AGCAGCCGAA CCAGCAGCCC GGAGGCAGCA GTTTGGACTC CGGGATCACC 
TCGCACCACC ATCCGGACAG CACCGACTCC TACGTCACCT ACCTGGAGAG CGATGACAAA 
TCCAAGCTGG CGCTGACCCC GTCGTCCTCT TCCTCGGCCT CGGCGGGCAC ATCGATCTCC 
TCGCCGCCAT CGGGCGTCGG AGCAGGGGGT GGCGGTGCCG TGGGCGGTGG TTCAGGTGTC 
CTGGGCTTGG GCGTGGTGGC CAATCAGTCG GCCACCGAGC AGCTCATGCA AATGCTCCAG 
AAGGTAACGG GCTCGGCTTC CCCGGCCTCA CATGCGGTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tup cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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