CG42750 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG42750
Entrez Gene ID	35090
Full Name	CG42750 gene product from transcript CG42750-RC
Synonyms	CG15157,CG15158,CG31746,CG31786,CG42400,CG7167,CG7198,Dmel\CG42750,Dmel_CG15157,Dmel_CG31746,Dmel_CG31786,Dmel_CG42400
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36E6-36F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165245.4	NP_724101.4	uncharacterized protein, isoform B
NM_001299103.1	NP_001286032.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

	DPEP2	XP_001097323.1
Bos taurus (cattle)	DPEP2	XP_002694951.1
Magnaporthe oryzae (rice blast fungus)	MGG_15247	XP_003712948.1
Xenopus tropicalis (tropical clawed frog)	dpep2	XP_004913598.1
Pan troglodytes (chimpanzee)	DPEP2	NP_001233413.1
Canis lupus familiaris (dog)	DPEP2	XP_853810.1
Drosophila melanogaster (fruit fly)	CG42750	NP_724101.4
Homo sapiens (human)	DPEP2	NP_071750.1
Rattus norvegicus (Norway rat)	Dpep2	NP_001011928.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007854	XP_317639.4
Mus musculus (house mouse)	Dpep2	NP_795887.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG42750 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG42750 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14881	NM_165245.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG42750), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa21672	NM_001299103.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG42750), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG42750 ( NM_165245.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724101.4 Drosophila melanogaster uncharacterized protein, transcript variant B (CG42750), mRNA.

Price & Availability↑

CloneID	OFa14881
Clone ID Related Accession (Same CDS sequence)	NM_165245.4
Accession Version	NM_165245.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 31, 2011 this sequence version replaced NM_165245.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC TAAGACGAAC TAATGGCCTG CACAAGCAGC ACTCCCTGAG AGATCGACAG 
GTGCATCCGA TGCTGCACGC CCTGGCGGAT CACGGCTGCA ACCTCAGCAG CGGTGGCAAC 
AGTGCGGCCA GCACCACCAG CAGTAGTACG GCCACGGCCA CCACAGCACT TGGTGGTGCC 
GGCGGTTTGG GCGGTCTGGG TGGCCTGAAC GGTGGTGTCG GCATAACAAG TCAGCGGAGC 
GTTGAGTTCG ATGAGCACGA CATCTTCTAC GTCTCACCAT CCAAGCGAAA AGGAGCCACT 
TCAGTAGGTT CCTCTGGCAA TGTGGTAACC TTCTCGAATT TTGTAAGTGA ACAACGCCTG 
ACGCCCTCGT CGTCGAACCG CGGATCCCTG AAGCGGAGCA AGGGCCGCTC CAATCAGTCC 
CTGTGCAGCT GCGATGCCGG CGACGAGGCC CAACTGGATT TGCAACCGAA TGCGGCAAGG 
CCGCTGTACG AGTACAGTCT GGAACGAAAG CGTAAGACAC ACACCTACTC CTGCGAGCAG 
AATGCGCAGA TACTTATGAG ACTGGAGCGC GAGCGTAATC GTAAATTATC GCTGACTGGG 
ATGGAAACGG GATTGGGAAT CGGACTGGGA GTGGGATTGG GAATGGGATC TGGAGGAGGA 
ACAGGAGGAG CCGGAGACCC CCAGAGCGAC ACGCCCAGCT TGTCGGATGT GGATGTGATA 
CCGCCTGTGC CACCACCCCC ATCGATGAAG CGCAATGGCA GCATGCGCAG CCTGCGATTG 
TTGCAACACC TGCAGCAGCA GCAGCAGCAG CAGCAGCAAA GCAGCCACAA TAACAACTTG 
GCGCAGCTGT GCAGCAGCGA TCTGTTGCAG GAGTGCACTA AGTCTGCATT GGATGAGTGC 
ACTGCCACAT TGCTCAAGTG CACCACGACC AGCAATCACA GCAGCAGCAA CAGCAATGCG 
AGCAACAATT GCAACATCGC TGCCAGCATT AGTCATAATA ATAACAATCA GGTGAAGCCG 
GACCTGTGCC AGCCGCCCGG CCACGCCCAC AACCATAGGC ACAAGCTGCA CGGCAATGCC 
ACGCCTACGC GCACGCCCAC ACCAACCACC ACCAACCCGC CACCGCCCCC GCCCCCTTTC 
GCCGCCCACG AGGAGGACGA GATATTCTCG CCGCACTCGA AGAAATCGGA CCCCAGCTTG 
TGCTTCCTGC CGGGCGGTAA TACATCCTCG AAATACAATA CCATTGGGCC GAGCAGCGAT 
TATGCACGGC ATTTGGCGCA CTACAGTCGC TACCTGCAGA AGCAGCACCA CCAGCAACAA 
ATGGCGCACT TTCAGCAGCA GCGCTGTCGC TGCTTCTCCC AGTCTTTGCT GAGTTTCCCG 
CAGCAACAAC AGCAACAGCA GCAACAACAG CAACATCAGC AGATGCAACA TGCGGAGCAA 
CAACAGCAGC ACAAGGCAGC CCAGCAGCCG GCCATCTTTC ACACCAGCAG CATGGATTGG 
TCGCTGCCAA TTAATAGTCG CAGCTTTGGC AATTTAGTGA ACATCGATGG TTCGGGCGGT 
ACTGGTGGTT GCCGTGTGCC GTACCAGAAA ATGCAATCGG GAAACGCAGG GTCAGGTGGT 
GCAATGGCAA ACGGCTGCTT GGGACTGGCC GCCTCGTCCA CATTTACGCT CACATCCTTC 
GACAGTGATA TCGGACACGG CCTGAAAGAA CGCGAGTCGC AGACATCGCT CCATCATCCG 
CACACGCTTC CGCATCCGCA TCCGCACACC CACTCGCATT CGCATCCGCA TCATTATCAC 
GCCCATGTGG GTGGTGCAGC GGTGAGTGGT GGGGTCGGCG GATCGGGTTC GGTGACGCCG 
CAGAAACACC ACCAGCTCCA CTCGAGCGTG ACGAGCATCA TGCCATGGAA GCACCGCCAG 
TGTCCCAGCA GGGGATCCTC CAGCTCCGGC ACGAATTCGT TCCGATTCGA TGCAGCCAAG 
CACTGGCTGG CAGTGAGCTC CATCCTCCTG ATAATTGGCG CTGCCAGTGT GGCCGTGCCA 
CTGGCGCTAC GTGTGGCAGC AAGTGCACCC TTTGAGGAGC GTTTGCGAGT GGCAGTTCAG 
CTTCTGGATC AAGTGCCTTT AATCGACGGG CATAACGACC TACCATGGAA TATTCGCAAG 
TTCCTTCACA ACAAACTCAA CGACTTCAAT TTTGATGAGG ATTTGCGCAA CGTAATGCCC 
TGGGGTCGCA GCCATTGGAG TCACACGGAC CTCACGCGAC TGAAGAAGGG ACGCATATCA 
GCACAGTTCT GGGCGGCCTA TGTCCCCTGC GAGGCTCAAC ATCGAGATGC AGTGCAGCTG 
ACTCTGGAGC AGATCGATGT AATAAAGCGC CTGACGGATC GGTATTCGCC GCAGCTTACC 
ACTTGCACTT CCGCACAGGA CATTATCGAT GCCCACAAGA ACCAGCAATT GTGCTCCCTG 
ACCGGCGTGG AGGGTGGTCA TTCCCTCGGG GGATCCCTGG CCGTACTACG CACCCTATAT 
GCCATTGGCG TCCGCTATAT GACGCTGACT TCAACCTGCC ACACCCCGTG GGCGGACTCG 
AGCTATGCGG ACGCGCCGAC CTTCAACATG AAGCACGGCG GCCTCACCAT TTTCGGCAAG 
ACAATCATCC GCGAAATGAA TCGACTGGGA ATGATGGTCG ATCTGTCCCA CGTCTCCAAG 
GGCACGATGA GGGACGCCCT GGAGGTGAGC GAGGCACCTG TGATATTCTC GCATTCCTCC 
GCCTACGAGC TGTGCAATAC GAGCCGAAAC GTCCAGGACG ACATCCTGCA GGCGCTGGCC 
AAGAACGGCG GCCTGGTCAT GGTCAACTTC TACTCGAAGT TCCTATCCTG CAGCGACAAC 
TCGACCGTGC ACGATGCAGT CGCGCATATT AATCACATTA AGCGAGTCGC AGGCATCGAT 
CACGTTGGAC TGGGCGCCGG CTACGACGGC ATTAATTATA CGCCCAAAGG ACTGGAGGAT 
GTGTCTTCAT ATCCGACTCT ATTTGCCGAA CTCCTTGGCG GCGGCTGGAC AATTGACGAG 
CTGACGAAGC TGGCAGGCGG CAATTTCCTC AGGGTCATGC AGCAGGTGGA AAAGCTGAGG 
GATGACAAAA AGGCAGCGGG CGTGAAACCC TTCGAGGATC ATCCGAACTT CCGCACGGAC 
GACCCGTACA ACTGTACGTC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724101.4
CDS	1852..5058
Translation	MTELRRTNGLHKQHSLRDRQVHPMLHALADHGCNLSSGGNSAASTTSSSTATATTALGGAGGLGGLGGLNGGVGITSQRSVEFDEHDIFYVSPSKRKGATSVGSSGNVVTFSNFVSEQRLTPSSSNRGSLKRSKGRSNQSLCSCDAGDEAQLDLQPNAARPLYEYSLERKRKTHTYSCEQNAQILMRLERERNRKLSLTGMETGLGIGLGVGLGMGSGGGTGGAGDPQSDTPSLSDVDVIPPVPPPPSMKRNGSMRSLRLLQHLQQQQQQQQQSSHNNNLAQLCSSDLLQECTKSALDECTATLLKCTTTSNHSSSNSNASNNCNIAASISHNNNNQVKPDLCQPPGHAHNHRHKLHGNATPTRTPTPTTTNPPPPPPPFAAHEEDEIFSPHSKKSDPSLCFLPGGNTSSKYNTIGPSSDYARHLAHYSRYLQKQHHQQQMAHFQQQRCRCFSQSLLSFPQQQQQQQQQQQHQQMQHAEQQQQHKAAQQPAIFHTSSMDWSLPINSRSFGNLVNIDGSGGTGGCRVPYQKMQSGNAGSGGAMANGCLGLAASSTFTLTSFDSDIGHGLKERESQTSLHHPHTLPHPHPHTHSHSHPHHYHAHVGGAAVSGGVGGSGSVTPQKHHQLHSSVTSIMPWKHRQCPSRGSSSSGTNSFRFDAAKHWLAVSSILLIIGAASVAVPLALRVAASAPFEERLRVAVQLLDQVPLIDGHNDLPWNIRKFLHNKLNDFNFDEDLRNVMPWGRSHWSHTDLTRLKKGRISAQFWAAYVPCEAQHRDAVQLTLEQIDVIKRLTDRYSPQLTTCTSAQDIIDAHKNQQLCSLTGVEGGHSLGGSLAVLRTLYAIGVRYMTLTSTCHTPWADSSYADAPTFNMKHGGLTIFGKTIIREMNRLGMMVDLSHVSKGTMRDALEVSEAPVIFSHSSAYELCNTSRNVQDDILQALAKNGGLVMVNFYSKFLSCSDNSTVHDAVAHINHIKRVAGIDHVGLGAGYDGINYTPKGLEDVSSYPTLFAELLGGGWTIDELTKLAGGNFLRVMQQVEKLRDDKKAAGVKPFEDHPNFRTDDPYNCTSS

Target ORF information:

RefSeq Version	NM_165245.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG42750), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165245.4

ORF Insert Sequence:

ATGACGGAGC TAAGACGAAC TAATGGCCTG CACAAGCAGC ACTCCCTGAG AGATCGACAG 
GTGCATCCGA TGCTGCACGC CCTGGCGGAT CACGGCTGCA ACCTCAGCAG CGGTGGCAAC 
AGTGCGGCCA GCACCACCAG CAGTAGTACG GCCACGGCCA CCACAGCACT TGGTGGTGCC 
GGCGGTTTGG GCGGTCTGGG TGGCCTGAAC GGTGGTGTCG GCATAACAAG TCAGCGGAGC 
GTTGAGTTCG ATGAGCACGA CATCTTCTAC GTCTCACCAT CCAAGCGAAA AGGAGCCACT 
TCAGTAGGTT CCTCTGGCAA TGTGGTAACC TTCTCGAATT TTGTAAGTGA ACAACGCCTG 
ACGCCCTCGT CGTCGAACCG CGGATCCCTG AAGCGGAGCA AGGGCCGCTC CAATCAGTCC 
CTGTGCAGCT GCGATGCCGG CGACGAGGCC CAACTGGATT TGCAACCGAA TGCGGCAAGG 
CCGCTGTACG AGTACAGTCT GGAACGAAAG CGTAAGACAC ACACCTACTC CTGCGAGCAG 
AATGCGCAGA TACTTATGAG ACTGGAGCGC GAGCGTAATC GTAAATTATC GCTGACTGGG 
ATGGAAACGG GATTGGGAAT CGGACTGGGA GTGGGATTGG GAATGGGATC TGGAGGAGGA 
ACAGGAGGAG CCGGAGACCC CCAGAGCGAC ACGCCCAGCT TGTCGGATGT GGATGTGATA 
CCGCCTGTGC CACCACCCCC ATCGATGAAG CGCAATGGCA GCATGCGCAG CCTGCGATTG 
TTGCAACACC TGCAGCAGCA GCAGCAGCAG CAGCAGCAAA GCAGCCACAA TAACAACTTG 
GCGCAGCTGT GCAGCAGCGA TCTGTTGCAG GAGTGCACTA AGTCTGCATT GGATGAGTGC 
ACTGCCACAT TGCTCAAGTG CACCACGACC AGCAATCACA GCAGCAGCAA CAGCAATGCG 
AGCAACAATT GCAACATCGC TGCCAGCATT AGTCATAATA ATAACAATCA GGTGAAGCCG 
GACCTGTGCC AGCCGCCCGG CCACGCCCAC AACCATAGGC ACAAGCTGCA CGGCAATGCC 
ACGCCTACGC GCACGCCCAC ACCAACCACC ACCAACCCGC CACCGCCCCC GCCCCCTTTC 
GCCGCCCACG AGGAGGACGA GATATTCTCG CCGCACTCGA AGAAATCGGA CCCCAGCTTG 
TGCTTCCTGC CGGGCGGTAA TACATCCTCG AAATACAATA CCATTGGGCC GAGCAGCGAT 
TATGCACGGC ATTTGGCGCA CTACAGTCGC TACCTGCAGA AGCAGCACCA CCAGCAACAA 
ATGGCGCACT TTCAGCAGCA GCGCTGTCGC TGCTTCTCCC AGTCTTTGCT GAGTTTCCCG 
CAGCAACAAC AGCAACAGCA GCAACAACAG CAACATCAGC AGATGCAACA TGCGGAGCAA 
CAACAGCAGC ACAAGGCAGC CCAGCAGCCG GCCATCTTTC ACACCAGCAG CATGGATTGG 
TCGCTGCCAA TTAATAGTCG CAGCTTTGGC AATTTAGTGA ACATCGATGG TTCGGGCGGT 
ACTGGTGGTT GCCGTGTGCC GTACCAGAAA ATGCAATCGG GAAACGCAGG GTCAGGTGGT 
GCAATGGCAA ACGGCTGCTT GGGACTGGCC GCCTCGTCCA CATTTACGCT CACATCCTTC 
GACAGTGATA TCGGACACGG CCTGAAAGAA CGCGAGTCGC AGACATCGCT CCATCATCCG 
CACACGCTTC CGCATCCGCA TCCGCACACC CACTCGCATT CGCATCCGCA TCATTATCAC 
GCCCATGTGG GTGGTGCAGC GGTGAGTGGT GGGGTCGGCG GATCGGGTTC GGTGACGCCG 
CAGAAACACC ACCAGCTCCA CTCGAGCGTG ACGAGCATCA TGCCATGGAA GCACCGCCAG 
TGTCCCAGCA GGGGATCCTC CAGCTCCGGC ACGAATTCGT TCCGATTCGA TGCAGCCAAG 
CACTGGCTGG CAGTGAGCTC CATCCTCCTG ATAATTGGCG CTGCCAGTGT GGCCGTGCCA 
CTGGCGCTAC GTGTGGCAGC AAGTGCACCC TTTGAGGAGC GTTTGCGAGT GGCAGTTCAG 
CTTCTGGATC AAGTGCCTTT AATCGACGGG CATAACGACC TACCATGGAA TATTCGCAAG 
TTCCTTCACA ACAAACTCAA CGACTTCAAT TTTGATGAGG ATTTGCGCAA CGTAATGCCC 
TGGGGTCGCA GCCATTGGAG TCACACGGAC CTCACGCGAC TGAAGAAGGG ACGCATATCA 
GCACAGTTCT GGGCGGCCTA TGTCCCCTGC GAGGCTCAAC ATCGAGATGC AGTGCAGCTG 
ACTCTGGAGC AGATCGATGT AATAAAGCGC CTGACGGATC GGTATTCGCC GCAGCTTACC 
ACTTGCACTT CCGCACAGGA CATTATCGAT GCCCACAAGA ACCAGCAATT GTGCTCCCTG 
ACCGGCGTGG AGGGTGGTCA TTCCCTCGGG GGATCCCTGG CCGTACTACG CACCCTATAT 
GCCATTGGCG TCCGCTATAT GACGCTGACT TCAACCTGCC ACACCCCGTG GGCGGACTCG 
AGCTATGCGG ACGCGCCGAC CTTCAACATG AAGCACGGCG GCCTCACCAT TTTCGGCAAG 
ACAATCATCC GCGAAATGAA TCGACTGGGA ATGATGGTCG ATCTGTCCCA CGTCTCCAAG 
GGCACGATGA GGGACGCCCT GGAGGTGAGC GAGGCACCTG TGATATTCTC GCATTCCTCC 
GCCTACGAGC TGTGCAATAC GAGCCGAAAC GTCCAGGACG ACATCCTGCA GGCGCTGGCC 
AAGAACGGCG GCCTGGTCAT GGTCAACTTC TACTCGAAGT TCCTATCCTG CAGCGACAAC 
TCGACCGTGC ACGATGCAGT CGCGCATATT AATCACATTA AGCGAGTCGC AGGCATCGAT 
CACGTTGGAC TGGGCGCCGG CTACGACGGC ATTAATTATA CGCCCAAAGG ACTGGAGGAT 
GTGTCTTCAT ATCCGACTCT ATTTGCCGAA CTCCTTGGCG GCGGCTGGAC AATTGACGAG 
CTGACGAAGC TGGCAGGCGG CAATTTCCTC AGGGTCATGC AGCAGGTGGA AAAGCTGAGG 
GATGACAAAA AGGCAGCGGG CGTGAAACCC TTCGAGGATC ATCCGAACTT CCGCACGGAC 
GACCCGTACA ACTGTACGTC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42750 ( NM_001299103.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286032.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG42750), mRNA.

Price & Availability↑

CloneID	OFa21672
Clone ID Related Accession (Same CDS sequence)	NM_001299103.1
Accession Version	NM_001299103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC TAAGACGAAC TAATGGCCTG CACAAGCAGC ACTCCCTGAG AGATCGACAG 
GTGCATCCGA TGCTGCACGC CCTGGCGGAT CACGGCTGCA ACCTCAGCAG CGGTGGCAAC 
AGTGCGGCCA GCACCACCAG CAGTAGTACG GCCACGGCCA CCACAGCACT TGGTGGTGCC 
GGCGGTTTGG GCGGTCTGGG TGGCCTGAAC GGTGGTGTCG GCATAACAAG TCAGCGGAGC 
GTTGAGTTCG ATGAGCACGA CATCTTCTAC GTCTCACCAT CCAAGCGAAA AGGAGCCACT 
TCAGGTTCCT CTGGCAATGT GGTAACCTTC TCGAATTTTG TAAGTGAACA ACGCCTGACG 
CCCTCGTCGT CGAACCGCGG ATCCCTGAAG CGGAGCAAGG GCCGCTCCAA TCAGTCCCTG 
TGCAGCTGCG ATGCCGGCGA CGAGGCCCAA CTGGATTTGC AACCGAATGC GGCAAGGCCG 
CTGTACGAGT ACAGTCTGGA ACGAAAGCGT AAGACACACA CCTACTCCTG CGAGCAGAAT 
GCGCAGATAC TTATGAGACT GGAGCGCGAG CGTAATCGTA AATTATCGCT GACTGGGATG 
GAAACGGGAT TGGGAATCGG ACTGGGAGTG GGATTGGGAA TGGGATCTGG AGGAGGAACA 
GGAGGAGCCG GAGACCCCCA GAGCGACACG CCCAGCTTGT CGGATGTGGA TGTGATACCG 
CCTGTGCCAC CACCCCCATC GATGAAGCGC AATGGCAGCA TGCGCAGCCT GCGATTGTTG 
CAACACCTGC AGCAGCAGCA GCAGCAGCAG CAGCAAAGCA GCCACAATAA CAACTTGGCG 
CAGCTGTGCA GCAGCGATCT GTTGCAGGAG TGCACTAAGT CTGCATTGGA TGAGTGCACT 
GCCACATTGC TCAAGTGCAC CACGACCAGC AATCACAGCA GCAGCAACAG CAATGCGAGC 
AACAATTGCA ACATCGCTGC CAGCATTAGT CATAATAATA ACAATCAGGT GAAGCCGGAC 
CTGTGCCAGC CGCCCGGCCA CGCCCACAAC CATAGGCACA AGCTGCACGG CAATGCCACG 
CCTACGCGCA CGCCCACACC AACCACCACC AACCCGCCAC CGCCCCCGCC CCCTTTCGCC 
GCCCACGAGG AGGACGAGAT ATTCTCGCCG CACTCGAAGA AATCGGACCC CAGCTTGTGC 
TTCCTGCCGG GCGGTAATAC ATCCTCGAAA TACAATACCA TTGGGCCGAG CAGCGATTAT 
GCACGGCATT TGGCGCACTA CAGTCGCTAC CTGCAGAAGC AGCACCACCA GCAACAAATG 
GCGCACTTTC AGCAGCAGCG CTGTCGCTGC TTCTCCCAGT CTTTGCTGAG TTTCCCGCAG 
CAACAACAGC AACAGCAGCA ACAACAGCAA CATCAGCAGA TGCAACATGC GGAGCAACAA 
CAGCAGCACA AGGCAGCCCA GCAGCCGGCC ATCTTTCACA CCAGCAGCAT GGATTGGTCG 
CTGCCAATTA ATAGTCGCAG CTTTGGCAAT TTAGTGAACA TCGATGGTTC GGGCGGTACT 
GGTGGTTGCC GTGTGCCGTA CCAGAAAATG CAATCGGGAA ACGCAGGGTC AGGTGGTGCA 
ATGGCAAACG GCTGCTTGGG ACTGGCCGCC TCGTCCACAT TTACGCTCAC ATCCTTCGAC 
AGTGATATCG GACACGGCCT GAAAGAACGC GAGTCGCAGA CATCGCTCCA TCATCCGCAC 
ACGCTTCCGC ATCCGCATCC GCACACCCAC TCGCATTCGC ATCCGCATCA TTATCACGCC 
CATGTGGGTG GTGCAGCGGT GAGTGGTGGG GTCGGCGGAT CGGGTTCGGT GACGCCGCAG 
AAACACCACC AGCTCCACTC GAGCGTGACG AGCATCATGC CATGGAAGCA CCGCCAGTGT 
CCCAGCAGGG GATCCTCCAG CTCCGGCACG AATTCGTTCC GATTCGATGC AGCCAAGCAC 
TGGCTGGCAG TGAGCTCCAT CCTCCTGATA ATTGGCGCTG CCAGTGTGGC CGTGCCACTG 
GCGCTACGTG TGGCAGCAAG TGCACCCTTT GAGGAGCGTT TGCGAGTGGC AGTTCAGCTT 
CTGGATCAAG TGCCTTTAAT CGACGGGCAT AACGACCTAC CATGGAATAT TCGCAAGTTC 
CTTCACAACA AACTCAACGA CTTCAATTTT GATGAGGATT TGCGCAACGT AATGCCCTGG 
GGTCGCAGCC ATTGGAGTCA CACGGACCTC ACGCGACTGA AGAAGGGACG CATATCAGCA 
CAGTTCTGGG CGGCCTATGT CCCCTGCGAG GCTCAACATC GAGATGCAGT GCAGCTGACT 
CTGGAGCAGA TCGATGTAAT AAAGCGCCTG ACGGATCGGT ATTCGCCGCA GCTTACCACT 
TGCACTTCCG CACAGGACAT TATCGATGCC CACAAGAACC AGCAATTGTG CTCCCTGACC 
GGCGTGGAGG GTGGTCATTC CCTCGGGGGA TCCCTGGCCG TACTACGCAC CCTATATGCC 
ATTGGCGTCC GCTATATGAC GCTGACTTCA ACCTGCCACA CCCCGTGGGC GGACTCGAGC 
TATGCGGACG CGCCGACCTT CAACATGAAG CACGGCGGCC TCACCATTTT CGGCAAGACA 
ATCATCCGCG AAATGAATCG ACTGGGAATG ATGGTCGATC TGTCCCACGT CTCCAAGGGC 
ACGATGAGGG ACGCCCTGGA GGTGAGCGAG GCACCTGTGA TATTCTCGCA TTCCTCCGCC 
TACGAGCTGT GCAATACGAG CCGAAACGTC CAGGACGACA TCCTGCAGGC GCTGGCCAAG 
AACGGCGGCC TGGTCATGGT CAACTTCTAC TCGAAGTTCC TATCCTGCAG CGACAACTCG 
ACCGTGCACG ATGCAGTCGC GCATATTAAT CACATTAAGC GAGTCGCAGG CATCGATCAC 
GTTGGACTGG GCGCCGGCTA CGACGGCATT AATTATACGC CCAAAGGACT GGAGGATGTG 
TCTTCATATC CGACTCTATT TGCCGAACTC CTTGGCGGCG GCTGGACAAT TGACGAGCTG 
ACGAAGCTGG CAGGCGGCAA TTTCCTCAGG GTCATGCAGC AGGTGGAAAA GCTGAGGGAT 
GACAAAAAGG CAGCGGGCGT GAAACCCTTC GAGGATCATC CGAACTTCCG CACGGACGAC 
CCGTACAACT GTACGTCCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286032.1
CDS	2092..5295
Translation	MTELRRTNGLHKQHSLRDRQVHPMLHALADHGCNLSSGGNSAASTTSSSTATATTALGGAGGLGGLGGLNGGVGITSQRSVEFDEHDIFYVSPSKRKGATSGSSGNVVTFSNFVSEQRLTPSSSNRGSLKRSKGRSNQSLCSCDAGDEAQLDLQPNAARPLYEYSLERKRKTHTYSCEQNAQILMRLERERNRKLSLTGMETGLGIGLGVGLGMGSGGGTGGAGDPQSDTPSLSDVDVIPPVPPPPSMKRNGSMRSLRLLQHLQQQQQQQQQSSHNNNLAQLCSSDLLQECTKSALDECTATLLKCTTTSNHSSSNSNASNNCNIAASISHNNNNQVKPDLCQPPGHAHNHRHKLHGNATPTRTPTPTTTNPPPPPPPFAAHEEDEIFSPHSKKSDPSLCFLPGGNTSSKYNTIGPSSDYARHLAHYSRYLQKQHHQQQMAHFQQQRCRCFSQSLLSFPQQQQQQQQQQQHQQMQHAEQQQQHKAAQQPAIFHTSSMDWSLPINSRSFGNLVNIDGSGGTGGCRVPYQKMQSGNAGSGGAMANGCLGLAASSTFTLTSFDSDIGHGLKERESQTSLHHPHTLPHPHPHTHSHSHPHHYHAHVGGAAVSGGVGGSGSVTPQKHHQLHSSVTSIMPWKHRQCPSRGSSSSGTNSFRFDAAKHWLAVSSILLIIGAASVAVPLALRVAASAPFEERLRVAVQLLDQVPLIDGHNDLPWNIRKFLHNKLNDFNFDEDLRNVMPWGRSHWSHTDLTRLKKGRISAQFWAAYVPCEAQHRDAVQLTLEQIDVIKRLTDRYSPQLTTCTSAQDIIDAHKNQQLCSLTGVEGGHSLGGSLAVLRTLYAIGVRYMTLTSTCHTPWADSSYADAPTFNMKHGGLTIFGKTIIREMNRLGMMVDLSHVSKGTMRDALEVSEAPVIFSHSSAYELCNTSRNVQDDILQALAKNGGLVMVNFYSKFLSCSDNSTVHDAVAHINHIKRVAGIDHVGLGAGYDGINYTPKGLEDVSSYPTLFAELLGGGWTIDELTKLAGGNFLRVMQQVEKLRDDKKAAGVKPFEDHPNFRTDDPYNCTSS

Target ORF information:

RefSeq Version	NM_001299103.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG42750), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299103.1

ORF Insert Sequence:

ATGACGGAGC TAAGACGAAC TAATGGCCTG CACAAGCAGC ACTCCCTGAG AGATCGACAG 
GTGCATCCGA TGCTGCACGC CCTGGCGGAT CACGGCTGCA ACCTCAGCAG CGGTGGCAAC 
AGTGCGGCCA GCACCACCAG CAGTAGTACG GCCACGGCCA CCACAGCACT TGGTGGTGCC 
GGCGGTTTGG GCGGTCTGGG TGGCCTGAAC GGTGGTGTCG GCATAACAAG TCAGCGGAGC 
GTTGAGTTCG ATGAGCACGA CATCTTCTAC GTCTCACCAT CCAAGCGAAA AGGAGCCACT 
TCAGGTTCCT CTGGCAATGT GGTAACCTTC TCGAATTTTG TAAGTGAACA ACGCCTGACG 
CCCTCGTCGT CGAACCGCGG ATCCCTGAAG CGGAGCAAGG GCCGCTCCAA TCAGTCCCTG 
TGCAGCTGCG ATGCCGGCGA CGAGGCCCAA CTGGATTTGC AACCGAATGC GGCAAGGCCG 
CTGTACGAGT ACAGTCTGGA ACGAAAGCGT AAGACACACA CCTACTCCTG CGAGCAGAAT 
GCGCAGATAC TTATGAGACT GGAGCGCGAG CGTAATCGTA AATTATCGCT GACTGGGATG 
GAAACGGGAT TGGGAATCGG ACTGGGAGTG GGATTGGGAA TGGGATCTGG AGGAGGAACA 
GGAGGAGCCG GAGACCCCCA GAGCGACACG CCCAGCTTGT CGGATGTGGA TGTGATACCG 
CCTGTGCCAC CACCCCCATC GATGAAGCGC AATGGCAGCA TGCGCAGCCT GCGATTGTTG 
CAACACCTGC AGCAGCAGCA GCAGCAGCAG CAGCAAAGCA GCCACAATAA CAACTTGGCG 
CAGCTGTGCA GCAGCGATCT GTTGCAGGAG TGCACTAAGT CTGCATTGGA TGAGTGCACT 
GCCACATTGC TCAAGTGCAC CACGACCAGC AATCACAGCA GCAGCAACAG CAATGCGAGC 
AACAATTGCA ACATCGCTGC CAGCATTAGT CATAATAATA ACAATCAGGT GAAGCCGGAC 
CTGTGCCAGC CGCCCGGCCA CGCCCACAAC CATAGGCACA AGCTGCACGG CAATGCCACG 
CCTACGCGCA CGCCCACACC AACCACCACC AACCCGCCAC CGCCCCCGCC CCCTTTCGCC 
GCCCACGAGG AGGACGAGAT ATTCTCGCCG CACTCGAAGA AATCGGACCC CAGCTTGTGC 
TTCCTGCCGG GCGGTAATAC ATCCTCGAAA TACAATACCA TTGGGCCGAG CAGCGATTAT 
GCACGGCATT TGGCGCACTA CAGTCGCTAC CTGCAGAAGC AGCACCACCA GCAACAAATG 
GCGCACTTTC AGCAGCAGCG CTGTCGCTGC TTCTCCCAGT CTTTGCTGAG TTTCCCGCAG 
CAACAACAGC AACAGCAGCA ACAACAGCAA CATCAGCAGA TGCAACATGC GGAGCAACAA 
CAGCAGCACA AGGCAGCCCA GCAGCCGGCC ATCTTTCACA CCAGCAGCAT GGATTGGTCG 
CTGCCAATTA ATAGTCGCAG CTTTGGCAAT TTAGTGAACA TCGATGGTTC GGGCGGTACT 
GGTGGTTGCC GTGTGCCGTA CCAGAAAATG CAATCGGGAA ACGCAGGGTC AGGTGGTGCA 
ATGGCAAACG GCTGCTTGGG ACTGGCCGCC TCGTCCACAT TTACGCTCAC ATCCTTCGAC 
AGTGATATCG GACACGGCCT GAAAGAACGC GAGTCGCAGA CATCGCTCCA TCATCCGCAC 
ACGCTTCCGC ATCCGCATCC GCACACCCAC TCGCATTCGC ATCCGCATCA TTATCACGCC 
CATGTGGGTG GTGCAGCGGT GAGTGGTGGG GTCGGCGGAT CGGGTTCGGT GACGCCGCAG 
AAACACCACC AGCTCCACTC GAGCGTGACG AGCATCATGC CATGGAAGCA CCGCCAGTGT 
CCCAGCAGGG GATCCTCCAG CTCCGGCACG AATTCGTTCC GATTCGATGC AGCCAAGCAC 
TGGCTGGCAG TGAGCTCCAT CCTCCTGATA ATTGGCGCTG CCAGTGTGGC CGTGCCACTG 
GCGCTACGTG TGGCAGCAAG TGCACCCTTT GAGGAGCGTT TGCGAGTGGC AGTTCAGCTT 
CTGGATCAAG TGCCTTTAAT CGACGGGCAT AACGACCTAC CATGGAATAT TCGCAAGTTC 
CTTCACAACA AACTCAACGA CTTCAATTTT GATGAGGATT TGCGCAACGT AATGCCCTGG 
GGTCGCAGCC ATTGGAGTCA CACGGACCTC ACGCGACTGA AGAAGGGACG CATATCAGCA 
CAGTTCTGGG CGGCCTATGT CCCCTGCGAG GCTCAACATC GAGATGCAGT GCAGCTGACT 
CTGGAGCAGA TCGATGTAAT AAAGCGCCTG ACGGATCGGT ATTCGCCGCA GCTTACCACT 
TGCACTTCCG CACAGGACAT TATCGATGCC CACAAGAACC AGCAATTGTG CTCCCTGACC 
GGCGTGGAGG GTGGTCATTC CCTCGGGGGA TCCCTGGCCG TACTACGCAC CCTATATGCC 
ATTGGCGTCC GCTATATGAC GCTGACTTCA ACCTGCCACA CCCCGTGGGC GGACTCGAGC 
TATGCGGACG CGCCGACCTT CAACATGAAG CACGGCGGCC TCACCATTTT CGGCAAGACA 
ATCATCCGCG AAATGAATCG ACTGGGAATG ATGGTCGATC TGTCCCACGT CTCCAAGGGC 
ACGATGAGGG ACGCCCTGGA GGTGAGCGAG GCACCTGTGA TATTCTCGCA TTCCTCCGCC 
TACGAGCTGT GCAATACGAG CCGAAACGTC CAGGACGACA TCCTGCAGGC GCTGGCCAAG 
AACGGCGGCC TGGTCATGGT CAACTTCTAC TCGAAGTTCC TATCCTGCAG CGACAACTCG 
ACCGTGCACG ATGCAGTCGC GCATATTAAT CACATTAAGC GAGTCGCAGG CATCGATCAC 
GTTGGACTGG GCGCCGGCTA CGACGGCATT AATTATACGC CCAAAGGACT GGAGGATGTG 
TCTTCATATC CGACTCTATT TGCCGAACTC CTTGGCGGCG GCTGGACAAT TGACGAGCTG 
ACGAAGCTGG CAGGCGGCAA TTTCCTCAGG GTCATGCAGC AGGTGGAAAA GCTGAGGGAT 
GACAAAAAGG CAGCGGGCGT GAAACCCTTC GAGGATCATC CGAACTTCCG CACGGACGAC 
CCGTACAACT GTACGTCCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG42750 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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