Socs36E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Socs36E
Entrez Gene ID	35085
Full Name	Suppressor of cytokine signaling at 36E
Synonyms	CG15154,Dmel\CG15154,SOCS,SOCS36E,SocS36E,Socs,anon-EST:Liang-2.2,clone 2.2,dSOCS,dmSocs36E,socs36E,socs36e,socs60E1
General protein information	Preferred Names Suppressor of cytokine signaling at 36E Names CG15154 gene product from transcript CG15154-RC CG15154-PA CG15154-PC CG15154-PD Socs36E-PA Socs36E-PC Socs36E-PD suppressor of cytokine signaling suppressor of cytokine signaling 36E suppressor of cytokine signaling at 36E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36E6-36E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273605.2	NP_001260534.1	suppressor of cytokine signaling at 36E, isoform C
NM_078869.6	NP_523593.5	suppressor of cytokine signaling at 36E, isoform D
NM_165243.3	NP_724096.1	suppressor of cytokine signaling at 36E, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Socs36E is node through which these processes are linked, demonstrating that negative feedback inhibition integrates multiple aspects of stem cell behavior.
Title: Socs36E Controls Niche Competition by Repressing MAPK Signaling in the Drosophila Testis.

contributes mechanistic insight to a Jak/STAT regulatory genetic circuit, and suggests that Socs36E regulates Jak/STAT signaling via a Cul2-dependent mechanism, as well as by a Cullin-independent manner, in vivo
Title: Socs36E limits STAT signaling via Cullin2 and a SOCS-box independent mechanism in the Drosophila egg chamber.

The Drosophila JAK/STAT pathway regulator SOCS36E functions via two independent mechanisms.
Title: Drosophila SOCS36E negatively regulates JAK/STAT pathway signaling via two separable mechanisms.

Socs36E attenuates STAT signaling to optimize motile cell specification in the Drosophila ovary.
Title: Socs36E attenuates STAT signaling to optimize motile cell specification in the Drosophila ovary.

study found the JAK-STAT signaling target & inhibitor, SOCS36E, is required for germline stem cell maintenance; SOCS36E suppresses JAK-STAT signaling specifically in the somatic stem cells, preventing them from displacing neighboring germline stem cells
Title: JAK-STAT signal inhibition regulates competition in the Drosophila testis stem cell niche.

The following Socs36E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Socs36E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14875	NM_001273605.2 Latest version!	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant C (Socs36E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa14875	NM_165243.3 Latest version!	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant A (Socs36E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa14876	NM_078869.6 Latest version!	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant D (Socs36E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Socs36E ( NM_001273605.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260534.1 Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant C (Socs36E), mRNA.

Price & Availability↑

CloneID	OFa14875
Clone ID Related Accession (Same CDS sequence)	NM_001273605.2 , NM_165243.3
Accession Version	NM_001273605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of cytokine signaling at 36E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273605.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260534.1
CDS	263..2164
Translation	MGHHLSKFSARANSQEKRNPTASSSPREAAALVETISLDEEELQVEEILTEATVSAATSPAEVRQIIIKIQLAKMENGNPVAAGQVESFAEVVEQEEEQQRQCADGATDDDAAALSADSLNANGQIVNVTLEGGYGEVGVVTNRTAGSSPEAAKTERQPVNNNGLAGSVGAAISTLQLHDNHGGNANCPLEADDDDDLDESLLRRRRSNSNSRSNIHHHHHHHQQQQQQQHEAEVVEYCEVLESLPVSNMLSAGTQHSSKPVSRSNNSNSCCRSRSSKALSMGAATSSASGSASTSVSTSTSSKRRCCKCKCRDAIRGLRDMLPSSSSSVSNKKDAAAVVAMVPRKSRTADRRSTPPTLGTSSGGSQRRMQSQRIRNSVAVPDASHHHHQLIIRITSPGFVVESPNGGRVTVVTEPAPQSPQTPPRPRPLATVAPAGGAGHAGIGAARNMTVHSQIDFMHCLVPDLEKITNSSFYWGKMDRYEAEHLLEGKPEGTFLLRDSAQEEFLFSVTFRKYGRSLHARIEQSGHKFSFDCHDPCVFTAPTVTGLLEHYKDPACVMFFEPCLTIPLHRRQTFSLQQLSRATIVSNTSYDGINQMELPGRLKSYLKEYHYKQKLRVKPIDQNTPMPYYGNV

Target ORF information:

RefSeq Version	NM_001273605.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant C (Socs36E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273605.2

ORF Insert Sequence:

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Socs36E ( NM_165243.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724096.1 Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant A (Socs36E), mRNA.

Price & Availability↑

CloneID	OFa14875
Clone ID Related Accession (Same CDS sequence)	NM_001273605.2 , NM_165243.3
Accession Version	NM_165243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of cytokine signaling at 36E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165243.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724096.1
CDS	432..2333
Translation	MGHHLSKFSARANSQEKRNPTASSSPREAAALVETISLDEEELQVEEILTEATVSAATSPAEVRQIIIKIQLAKMENGNPVAAGQVESFAEVVEQEEEQQRQCADGATDDDAAALSADSLNANGQIVNVTLEGGYGEVGVVTNRTAGSSPEAAKTERQPVNNNGLAGSVGAAISTLQLHDNHGGNANCPLEADDDDDLDESLLRRRRSNSNSRSNIHHHHHHHQQQQQQQHEAEVVEYCEVLESLPVSNMLSAGTQHSSKPVSRSNNSNSCCRSRSSKALSMGAATSSASGSASTSVSTSTSSKRRCCKCKCRDAIRGLRDMLPSSSSSVSNKKDAAAVVAMVPRKSRTADRRSTPPTLGTSSGGSQRRMQSQRIRNSVAVPDASHHHHQLIIRITSPGFVVESPNGGRVTVVTEPAPQSPQTPPRPRPLATVAPAGGAGHAGIGAARNMTVHSQIDFMHCLVPDLEKITNSSFYWGKMDRYEAEHLLEGKPEGTFLLRDSAQEEFLFSVTFRKYGRSLHARIEQSGHKFSFDCHDPCVFTAPTVTGLLEHYKDPACVMFFEPCLTIPLHRRQTFSLQQLSRATIVSNTSYDGINQMELPGRLKSYLKEYHYKQKLRVKPIDQNTPMPYYGNV

Target ORF information:

RefSeq Version	NM_165243.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant A (Socs36E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165243.3

ORF Insert Sequence:

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Socs36E ( NM_078869.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523593.5 Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant D (Socs36E), mRNA.

Price & Availability↑

CloneID	OFa14876
Clone ID Related Accession (Same CDS sequence)	NM_078869.6
Accession Version	NM_078869.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of cytokine signaling at 36E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078869.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AGCGGGAGGA GCAGCAGGTG 
GGCTTCACCT ACTACGAGGA CGTGGAGAGC GGCAGCGAGG TGGACGAGGA CGACGACGAG 
GGGGTCAACA TACGGGTCTC CTACGTTCAG CACGCCGAGG TGGTGGCCCA GCTGCAGCAG 
GTCACCACGG CGTCGGGCCC ATCGGCTCCG TCCTCATCCA GATCGTCCAA TCTTCAGGAG 
CACCTCAGCA GGCTGCCCAA CCGCATGGCA GCCGCCTTTG GTCGGATGTC GGCCAATCTG 
AATTGCTACC AGAAGACTGT CGCCACGCCG GAGCGTCACT CCAACTCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523593.5
CDS	432..2645
Translation	MGHHLSKFSARANSQEKRNPTASSSPREAAALVETISLDEEELQVEEILTEATVSAATSPAEVRQIIIKIQLAKMENGNPVAAGQVESFAEVVEQEEEQQRQCADGATDDDAAALSADSLNANGQIVNVTLEGGYGEVGVVTNRTAGSSPEAAKTERQPVNNNGLAGSVGAAISTLQLHDNHGGNANCPLEADDDDDLDESLLRRRRSNSNSRSNIHHHHHHHQQQQQQQHEAEVVEYCEVLESLPVSNMLSAGTQHSSKPVSRSNNSNSCCRSRSSKALSMGAATSSASGSASTSVSTSTSSKRRCCKCKCRDAIRGLRDMLPSSSSSVSNKKDAAAVVAMVPRKSRTADRRSTPPTLGTSSGGSQRRMQSQRIRNSVAVPDASHHHHQLIIRITSPGFVVESPNGGRVTVVTEPAPQSPQTPPRPRPLATVAPAGGAGHAGIGAARNMTVHSQIDFMHCLVPDLEKITNSSFYWGKMDRYEAEHLLEGKPEGTFLLRDSAQEEFLFSVTFRKYGRSLHARIEQSGHKFSFDCHDPCVFTAPTVTGLLEHYKDPACVMFFEPCLTIPLHRRQTFSLQQLSRATIVSNTSYDGINQMELPGRLKSYLKEYHYKQKLRVKPIDQNTPMPYYGNV*REEQQVGFTYYEDVESGSEVDEDDDEGVNIRVSYVQHAEVVAQLQQVTTASGPSAPSSSRSSNLQEHLSRLPNRMAAAFGRMSANLNCYQKTVATPERHSNSS

Target ORF information:

RefSeq Version	NM_078869.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of cytokine signaling at 36E, transcript variant D (Socs36E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078869.6

ORF Insert Sequence:

ATGGGTCATC ACCTTAGCAA GTTCTCAGCA CGCGCCAATA GCCAGGAAAA GAGAAATCCT 
ACCGCCAGCT CGAGCCCCAG GGAAGCAGCA GCACTAGTAG AGACGATCAG CCTGGACGAG 
GAGGAGCTGC AGGTGGAAGA GATCCTCACA GAGGCCACTG TCTCAGCCGC CACATCGCCG 
GCGGAAGTGC GTCAGATCAT CATCAAAATC CAGCTGGCCA AAATGGAGAA CGGCAATCCG 
GTGGCCGCTG GTCAGGTGGA AAGTTTCGCC GAGGTGGTGG AACAGGAGGA GGAGCAGCAG 
CGGCAATGCG CAGATGGTGC CACCGACGAC GACGCCGCCG CCCTGTCTGC GGATTCGCTG 
AATGCAAATG GGCAGATTGT GAATGTGACG CTGGAGGGCG GCTACGGCGA GGTGGGCGTG 
GTGACAAATC GAACTGCTGG CAGTTCGCCG GAAGCGGCCA AAACCGAACG ACAGCCCGTG 
AATAACAACG GTTTAGCGGG AAGCGTCGGC GCTGCGATAA GTACGTTACA ACTGCACGAC 
AACCATGGCG GCAACGCCAA CTGCCCCTTG GAAGCCGACG ACGATGACGA CCTGGATGAG 
TCGCTGTTGC GTCGGCGTCG CAGCAACAGC AACAGCCGCA GCAACATCCA CCACCACCAC 
CACCACCACC AGCAGCAGCA GCAGCAGCAG CACGAGGCCG AAGTCGTCGA GTATTGCGAA 
GTGCTCGAGT CGCTGCCAGT CAGCAATATG TTGTCGGCGG GAACGCAACA CAGCAGCAAG 
CCAGTTTCCA GAAGCAATAA TAGCAACAGT TGCTGCCGCA GTCGCAGCAG TAAAGCACTT 
TCAATGGGAG CAGCAACATC CTCCGCCTCC GGATCCGCAT CCACATCCGT GTCCACATCC 
ACATCCTCGA AGCGACGATG CTGCAAGTGC AAGTGCCGCG ACGCCATCCG AGGATTAAGG 
GATATGCTGC CGAGCAGCAG CAGTTCCGTG AGCAATAAAA AGGATGCCGC TGCGGTGGTG 
GCGATGGTTC CGAGGAAATC CCGGACAGCT GACCGCCGCT CCACACCGCC CACGCTGGGC 
ACATCGAGTG GTGGCTCACA GCGCCGGATG CAGAGCCAAA GGATACGAAA TTCCGTCGCC 
GTTCCGGACG CCAGCCATCA CCATCATCAG CTCATCATTC GGATCACATC GCCGGGCTTC 
GTGGTCGAGT CGCCCAATGG CGGCCGCGTA ACTGTGGTCA CCGAGCCCGC CCCCCAGTCG 
CCCCAGACTC CGCCCCGGCC ACGTCCCCTG GCCACGGTCG CCCCCGCCGG AGGGGCTGGC 
CATGCCGGAA TCGGAGCCGC CCGCAACATG ACCGTGCACT CGCAAATCGA CTTCATGCAC 
TGCCTGGTTC CCGATCTCGA GAAGATCACG AACAGCAGCT TCTACTGGGG CAAGATGGAC 
CGCTACGAGG CGGAGCACCT GCTCGAGGGC AAGCCGGAGG GCACGTTCCT GCTGCGCGAC 
TCCGCCCAGG AGGAGTTCCT CTTCTCGGTC ACATTCCGGA AGTACGGCAG GTCACTGCAC 
GCTCGCATCG AGCAGAGCGG TCACAAGTTC AGCTTCGACT GCCACGATCC GTGTGTGTTC 
ACGGCGCCCA CGGTGACAGG ACTTTTGGAG CACTACAAGG ACCCCGCCTG CGTGATGTTC 
TTCGAGCCCT GCCTGACGAT ACCCCTGCAC AGAAGGCAGA CCTTCTCCCT GCAGCAGCTC 
TCCCGGGCGA CCATTGTGTC CAACACCAGC TACGACGGGA TCAACCAGAT GGAGCTGCCC 
GGTCGGCTGA AGAGCTACCT TAAGGAGTAT CACTACAAGC AGAAGCTGCG CGTCAAGCCC 
ATCGACCAGA ACACCCCGAT GCCGTACTAC GGCAATGTAT AGCGGGAGGA GCAGCAGGTG 
GGCTTCACCT ACTACGAGGA CGTGGAGAGC GGCAGCGAGG TGGACGAGGA CGACGACGAG 
GGGGTCAACA TACGGGTCTC CTACGTTCAG CACGCCGAGG TGGTGGCCCA GCTGCAGCAG 
GTCACCACGG CGTCGGGCCC ATCGGCTCCG TCCTCATCCA GATCGTCCAA TCTTCAGGAG 
CACCTCAGCA GGCTGCCCAA CCGCATGGCA GCCGCCTTTG GTCGGATGTC GGCCAATCTG 
AATTGCTACC AGAAGACTGT CGCCACGCCG GAGCGTCACT CCAACTCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Socs36E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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