B4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	B4
Entrez Gene ID	34767
Full Name	CG9239 gene product from transcript CG9239-RC
Synonyms	B4/Susi,BG:DS07660.4,CG9239,CG9260,Dmel\CG9239,Susi,n(2)05337
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34C3-34C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057977.4	NP_477325.2	B4, isoform A
NM_001273516.1	NP_001260445.1	B4, isoform C
NM_165046.1	NP_723823.1	B4, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	B4	NP_001260445.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007774	XP_317741.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Susi, a negative regulator of Drosophila PI3-kinase
Title: Susi, a negative regulator of Drosophila PI3-kinase.

The following B4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14666	NM_165046.1 Latest version!	Drosophila melanogaster B4, transcript variant B (B4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa14666	NM_057977.4 Latest version!	Drosophila melanogaster B4, transcript variant A (B4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa14666	NM_001273516.1 Latest version!	Drosophila melanogaster B4, transcript variant C (B4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B4 ( NM_057977.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477325.2 Drosophila melanogaster B4, transcript variant A (B4), mRNA.

Price & Availability↑

CloneID	OFa14666
Clone ID Related Accession (Same CDS sequence)	NM_057977.4 , NM_001273516.1 , NM_165046.1
Accession Version	NM_057977.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	B4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057977.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477325.2
CDS	749..4072
Translation	MNETLKLSGLGMQRTNIELERQRHLANWLMNSRPHMPGGPAAATTTSAAAATTPTASATKGATTTTATTTNPGSTVGGAGAGPVGGNGVATAAAAGAPSNSVAAAAAAACNGGAAVGAHHGHHSAAGARKPKLRRFNSHDTSSNMFSVADFENARLARRNEIELNQRRARRVRGSANNSTYGLGSGSGLLGGAHSGYCDALNGSGDYSTGDSKASKGSSEGALTAIEFLERNSLPRVVRILHASKSSNETLQSSSSHGSSTAASSSSSTGANPFGSGGSGGGNISSGNIDTGTHSSSSGGSATGNGTGSGPGLSSGSGSGSGSGHQNNNGRHSSNGGTPPGHDELFLLYRLVRQRNIYHGHNAKTQASQRKKTLLIPQEFPGYFSMLSEKGLPTATQYGSLIQLVRERVYKFVSVDNMPAFTESSPNSTSSPTHEGCLPINKGRPQYVKTTARGGQVFRLLAVFEDGKQDQVNMSQHGNGNATRSHGSGYMGREKEKNRYAQLLNENRQVLYVPLSTKGKFYEIEPGIPQLLQKLGDAQRKLNPECVHRLSALVTAAKQLPLTLRYISGPGGPDIPEQLCISQVSKEDVVVGCSIEDVDTQTPLQLRKFYPSRDIQLVKSYLGFDSEQRMLANTNVQNMLKFCQFNCDPFLKLVEIELIQRSERSGSKNREGLRILKPLHFPKILRREKSSMAAAHEKEDSIIFLSKSDLENLEAKEAAASAAAHSESSLNRISERMRVFQSTKKKWFGKHHPQQQQPQIEKHQSDLDLDEQAKRMSMERYTDMSKLLQERFGSNPEQPEQDAISLNSTAASDTETTMLTTMEPRSLDTMMQKSMSLQDIELLNGQCKQRPDLLASHNHNDAISEAGTELEDVENQTPPPQSFITEKLYNEFHVKTRQYSKSSSSLHQLPLFSLPQKLQLHEAEKEKRHLMLLKAQQQALTKEPVTGRRAAGGVSLLGGGLNQLAISPASLVEDDLPYSSVRDSLVISGGDCLRSESASRPVPAVDYTKENIYAEICPSHTVDTFSGATQFTHVPDDDGDYASLKYSVNGPQSVSRIEVNGATAAVKSSAISYHTVYLGEEPLGERHAAHITTVELAGEDNIYNTLK

Target ORF information:

RefSeq Version	NM_057977.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster B4, transcript variant A (B4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057977.4

ORF Insert Sequence:

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4 ( NM_001273516.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260445.1 Drosophila melanogaster B4, transcript variant C (B4), mRNA.

Price & Availability↑

CloneID	OFa14666
Clone ID Related Accession (Same CDS sequence)	NM_057977.4 , NM_001273516.1 , NM_165046.1
Accession Version	NM_001273516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	B4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260445.1
CDS	891..4214
Translation	MNETLKLSGLGMQRTNIELERQRHLANWLMNSRPHMPGGPAAATTTSAAAATTPTASATKGATTTTATTTNPGSTVGGAGAGPVGGNGVATAAAAGAPSNSVAAAAAAACNGGAAVGAHHGHHSAAGARKPKLRRFNSHDTSSNMFSVADFENARLARRNEIELNQRRARRVRGSANNSTYGLGSGSGLLGGAHSGYCDALNGSGDYSTGDSKASKGSSEGALTAIEFLERNSLPRVVRILHASKSSNETLQSSSSHGSSTAASSSSSTGANPFGSGGSGGGNISSGNIDTGTHSSSSGGSATGNGTGSGPGLSSGSGSGSGSGHQNNNGRHSSNGGTPPGHDELFLLYRLVRQRNIYHGHNAKTQASQRKKTLLIPQEFPGYFSMLSEKGLPTATQYGSLIQLVRERVYKFVSVDNMPAFTESSPNSTSSPTHEGCLPINKGRPQYVKTTARGGQVFRLLAVFEDGKQDQVNMSQHGNGNATRSHGSGYMGREKEKNRYAQLLNENRQVLYVPLSTKGKFYEIEPGIPQLLQKLGDAQRKLNPECVHRLSALVTAAKQLPLTLRYISGPGGPDIPEQLCISQVSKEDVVVGCSIEDVDTQTPLQLRKFYPSRDIQLVKSYLGFDSEQRMLANTNVQNMLKFCQFNCDPFLKLVEIELIQRSERSGSKNREGLRILKPLHFPKILRREKSSMAAAHEKEDSIIFLSKSDLENLEAKEAAASAAAHSESSLNRISERMRVFQSTKKKWFGKHHPQQQQPQIEKHQSDLDLDEQAKRMSMERYTDMSKLLQERFGSNPEQPEQDAISLNSTAASDTETTMLTTMEPRSLDTMMQKSMSLQDIELLNGQCKQRPDLLASHNHNDAISEAGTELEDVENQTPPPQSFITEKLYNEFHVKTRQYSKSSSSLHQLPLFSLPQKLQLHEAEKEKRHLMLLKAQQQALTKEPVTGRRAAGGVSLLGGGLNQLAISPASLVEDDLPYSSVRDSLVISGGDCLRSESASRPVPAVDYTKENIYAEICPSHTVDTFSGATQFTHVPDDDGDYASLKYSVNGPQSVSRIEVNGATAAVKSSAISYHTVYLGEEPLGERHAAHITTVELAGEDNIYNTLK

Target ORF information:

RefSeq Version	NM_001273516.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster B4, transcript variant C (B4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273516.1

ORF Insert Sequence:

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B4 ( NM_165046.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723823.1 Drosophila melanogaster B4, transcript variant B (B4), mRNA.

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CloneID	OFa14666
Clone ID Related Accession (Same CDS sequence)	NM_057977.4 , NM_001273516.1 , NM_165046.1
Accession Version	NM_165046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	B4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723823.1
CDS	572..3895
Translation	MNETLKLSGLGMQRTNIELERQRHLANWLMNSRPHMPGGPAAATTTSAAAATTPTASATKGATTTTATTTNPGSTVGGAGAGPVGGNGVATAAAAGAPSNSVAAAAAAACNGGAAVGAHHGHHSAAGARKPKLRRFNSHDTSSNMFSVADFENARLARRNEIELNQRRARRVRGSANNSTYGLGSGSGLLGGAHSGYCDALNGSGDYSTGDSKASKGSSEGALTAIEFLERNSLPRVVRILHASKSSNETLQSSSSHGSSTAASSSSSTGANPFGSGGSGGGNISSGNIDTGTHSSSSGGSATGNGTGSGPGLSSGSGSGSGSGHQNNNGRHSSNGGTPPGHDELFLLYRLVRQRNIYHGHNAKTQASQRKKTLLIPQEFPGYFSMLSEKGLPTATQYGSLIQLVRERVYKFVSVDNMPAFTESSPNSTSSPTHEGCLPINKGRPQYVKTTARGGQVFRLLAVFEDGKQDQVNMSQHGNGNATRSHGSGYMGREKEKNRYAQLLNENRQVLYVPLSTKGKFYEIEPGIPQLLQKLGDAQRKLNPECVHRLSALVTAAKQLPLTLRYISGPGGPDIPEQLCISQVSKEDVVVGCSIEDVDTQTPLQLRKFYPSRDIQLVKSYLGFDSEQRMLANTNVQNMLKFCQFNCDPFLKLVEIELIQRSERSGSKNREGLRILKPLHFPKILRREKSSMAAAHEKEDSIIFLSKSDLENLEAKEAAASAAAHSESSLNRISERMRVFQSTKKKWFGKHHPQQQQPQIEKHQSDLDLDEQAKRMSMERYTDMSKLLQERFGSNPEQPEQDAISLNSTAASDTETTMLTTMEPRSLDTMMQKSMSLQDIELLNGQCKQRPDLLASHNHNDAISEAGTELEDVENQTPPPQSFITEKLYNEFHVKTRQYSKSSSSLHQLPLFSLPQKLQLHEAEKEKRHLMLLKAQQQALTKEPVTGRRAAGGVSLLGGGLNQLAISPASLVEDDLPYSSVRDSLVISGGDCLRSESASRPVPAVDYTKENIYAEICPSHTVDTFSGATQFTHVPDDDGDYASLKYSVNGPQSVSRIEVNGATAAVKSSAISYHTVYLGEEPLGERHAAHITTVELAGEDNIYNTLK

Target ORF information:

RefSeq Version	NM_165046.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster B4, transcript variant B (B4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165046.1

ORF Insert Sequence:

ATGAACGAGA CCTTAAAGCT GTCCGGACTC GGAATGCAGC GCACCAATAT CGAGCTCGAG 
CGGCAGAGGC ACCTGGCCAA CTGGCTGATG AACAGTAGAC CGCACATGCC CGGCGGACCA 
GCAGCAGCAA CAACAACATC AGCGGCAGCA GCAACAACAC CCACTGCATC AGCCACAAAG 
GGCGCAACAA CAACAACAGC TACAACTACA AATCCTGGCT CCACAGTGGG TGGAGCAGGC 
GCTGGCCCAG TGGGCGGCAA CGGGGTCGCA ACAGCAGCAG CAGCGGGCGC ACCCAGCAAC 
AGCGTCGCAG CTGCAGCAGC AGCTGCATGT AACGGTGGAG CAGCCGTTGG CGCCCATCAC 
GGCCACCATT CGGCGGCTGG GGCGCGCAAG CCCAAACTGC GGCGCTTCAA CTCCCACGAC 
ACCAGTTCGA ATATGTTCTC GGTGGCCGAT TTCGAGAACG CCCGCCTAGC TCGCCGCAAC 
GAGATCGAGC TGAACCAGCG GAGGGCACGA CGCGTGCGCG GCAGTGCCAA CAACAGCACC 
TACGGCCTGG GATCGGGCTC AGGATTGCTG GGAGGTGCCC ATTCTGGATA CTGTGACGCA 
CTCAATGGCA GCGGCGATTA CTCCACCGGG GACAGCAAGG CCTCCAAGGG CAGCAGCGAA 
GGTGCGCTGA CAGCCATTGA GTTCCTTGAG CGGAATTCCC TGCCACGAGT CGTCCGCATT 
CTGCATGCGA GCAAGAGCTC AAACGAAACG CTGCAGTCCA GCTCATCGCA TGGCTCCTCC 
ACGGCGGCAT CGAGCAGCTC CTCGACGGGA GCGAATCCGT TTGGTTCTGG TGGCAGTGGT 
GGTGGAAACA TTTCCAGTGG CAACATTGAC ACCGGCACCC ATTCATCGTC CAGCGGAGGC 
AGCGCCACCG GAAACGGAAC TGGCTCTGGT CCTGGTCTTA GTTCGGGCTC GGGTTCTGGT 
TCTGGTTCTG GTCACCAGAA CAACAACGGT CGCCACAGCA GCAACGGTGG CACTCCACCC 
GGCCACGATG AGCTTTTTCT GCTCTACAGG CTGGTGCGAC AGCGCAACAT CTACCACGGA 
CACAACGCCA AGACTCAGGC ATCGCAGCGC AAGAAGACTC TGCTCATTCC ACAGGAGTTT 
CCGGGTTACT TTTCGATGCT AAGCGAGAAG GGTCTCCCCA CGGCCACACA ATACGGATCT 
CTGATACAGC TGGTTCGGGA ACGGGTTTAC AAGTTTGTCT CAGTGGACAA CATGCCGGCG 
TTTACGGAAT CCTCGCCCAA CAGTACGAGC AGTCCAACGC ACGAGGGATG CCTGCCCATA 
AACAAGGGAC GACCACAGTA CGTGAAAACG ACGGCAAGGG GCGGGCAGGT ATTCCGTCTG 
TTGGCCGTCT TCGAGGATGG CAAACAGGAT CAGGTCAATA TGTCGCAACA TGGCAACGGC 
AATGCGACCA GGTCCCATGG CAGTGGGTAC ATGGGAAGGG AGAAGGAAAA GAACCGGTAT 
GCCCAGCTGC TTAATGAAAA CCGGCAGGTG TTGTACGTCC CACTGTCCAC CAAGGGCAAG 
TTCTACGAGA TTGAACCGGG AATTCCTCAG CTGCTTCAGA AACTGGGCGA TGCGCAGCGC 
AAGCTGAATC CGGAGTGCGT GCATCGTTTG AGTGCTTTGG TGACGGCGGC CAAGCAGTTA 
CCGCTAACAC TGCGCTATAT CTCCGGTCCA GGCGGACCCG ACATTCCCGA GCAGCTATGT 
ATTAGTCAAG TGAGCAAGGA GGATGTGGTT GTTGGCTGTT CCATTGAGGA CGTCGATACC 
CAGACGCCCT TGCAGCTGAG GAAGTTCTAT CCCAGTCGAG ATATTCAGCT GGTAAAAAGT 
TATTTAGGAT TTGACTCCGA ACAACGGATG CTCGCTAACA CGAATGTGCA GAATATGCTA 
AAGTTTTGTC AATTTAATTG CGATCCATTC CTGAAGCTCG TGGAGATTGA GTTAATTCAG 
CGCTCGGAAC GTTCGGGAAG CAAGAATCGA GAGGGGCTAC GAATCTTGAA GCCACTGCAT 
TTTCCAAAGA TCCTGAGACG CGAGAAGAGC TCCATGGCAG CAGCGCACGA AAAGGAGGAT 
TCCATTATTT TCCTCAGCAA ATCGGATCTG GAGAATCTGG AAGCCAAGGA AGCGGCAGCT 
TCTGCTGCAG CTCACTCGGA ATCCAGTTTG AATCGCATCT CTGAGCGCAT GCGTGTATTC 
CAGTCTACCA AGAAGAAGTG GTTCGGAAAG CATCATCCGC AGCAACAGCA ACCACAAATA 
GAGAAACATC AATCTGACTT AGATCTCGAT GAGCAGGCCA AGCGAATGAG CATGGAGCGC 
TATACGGATA TGTCTAAGCT TCTGCAGGAG CGTTTTGGCT CGAATCCCGA ACAGCCAGAG 
CAAGATGCTA TCTCCCTAAA CAGCACAGCA GCGTCCGACA CCGAAACAAC GATGCTGACT 
ACCATGGAGC CGAGGTCTCT GGACACAATG ATGCAAAAGT CCATGTCCCT GCAGGATATT 
GAGCTTCTCA ATGGTCAATG CAAGCAACGA CCTGATTTGC TGGCCAGCCA CAATCACAAT 
GACGCCATCA GTGAGGCAGG TACCGAGCTG GAAGATGTGG AGAATCAGAC CCCACCTCCA 
CAGTCCTTCA TCACGGAGAA GCTGTACAAC GAGTTTCACG TGAAGACCAG GCAGTATAGC 
AAGTCATCGT CCAGTTTGCA CCAGTTACCT CTGTTTAGCT TGCCGCAAAA GTTGCAACTT 
CACGAGGCTG AAAAGGAGAA GCGACATCTG ATGTTGCTAA AGGCGCAGCA ACAGGCGCTG 
ACCAAGGAAC CGGTCACTGG AAGAAGGGCA GCCGGTGGAG TTTCTCTGTT GGGAGGAGGA 
CTCAACCAAC TGGCGATATC GCCCGCATCG CTGGTGGAAG ACGACTTGCC CTACTCCAGT 
GTGCGCGATT CGCTTGTGAT TTCCGGCGGG GACTGCCTGC GATCGGAGTC CGCCAGTAGA 
CCAGTTCCCG CTGTGGACTA TACCAAGGAG AATATATACG CCGAAATCTG TCCCTCGCAC 
ACAGTGGACA CCTTTTCGGG CGCCACTCAG TTCACTCATG TGCCCGACGA TGATGGAGAT 
TATGCATCGC TCAAGTATTC GGTCAATGGA CCACAGTCGG TCAGTCGGAT TGAGGTGAAT 
GGAGCTACGG CGGCGGTGAA GAGCAGCGCA ATCAGCTACC ATACTGTGTA CCTGGGCGAG 
GAACCGCTGG GCGAGAGGCA CGCTGCCCAC ATCACCACCG TTGAATTGGC CGGAGAGGAT 
AACATTTACA ATACGCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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B4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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