Sirt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sirt1
Entrez Gene ID	34708
Full Name	Sirtuin 1
Synonyms	BEST:LD38188,CG5216,D.mel1,DSir2,Dmel\CG5216,SIR2,Sir2,Sir2L,dSIR2,dSir2,dmSRT406,dsir2,l(2)05326,sir2
General protein information	Preferred Names Sirtuin 1 Names CG5216 gene product from transcript CG5216-RA CG5216-PA Sir2 Sirt1-PA silent information regulator 2 sirtuin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A7-34A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_058003.4	NP_477351.1	sirtuin 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The role of Sirt1 in Notch signalling is, therefore, more complex than previously recognized, and its diverse effects may be explained by a plethora of Sirt1 substrates involved in the regulation of Notch signalling.
Title: The silent information regulator 1 (Sirt1) is a positive regulator of the Notch pathway in Drosophila.

Sir2 in the mushroom bodies promotes short-term ethanol-induced behavioral plasticity by allowing changes in the expression of presynaptic molecules.
Title: Sir2/Sirt1 Links Acute Inebriation to Presynaptic Changes and the Development of Alcohol Tolerance, Preference, and Reward.

A lowered gene dosage of Sir2, in mutant heterozygotes, can extend normal longevity and greatly augment survivorship during amino-acid starvation in Drosophila.
Title: Extended longevity and survivorship during amino-acid starvation in a Drosophila Sir2 mutant heterozygote.

Drosophila HNF4 mutants display diabetic phenotypes similar to those of sir2 mutants, and protein levels for dHNF4 are reduced in sir2 mutant animals. Sir2 exerts these effects by deacetylating and stabilizing dHNF4 through protein interactions.
Title: Sir2 Acts through Hepatocyte Nuclear Factor 4 to maintain insulin Signaling and Metabolic Homeostasis in Drosophila.

Low oxygen partial pressure of highly resistant Drosophila significantly reduces the time of restitution and increases the expression of Sir2 and CG14740 genes.
Title: [The effect of altered oxygen partial pressure on the resisitance to hypoxia and expression of oxygen-sensitive genes in Drosophila melanogaster].

The following Sirt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sirt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14599	NM_058003.4 Latest version!	Drosophila melanogaster sirtuin 1 (Sirt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sirt1 ( NM_058003.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477351.1 Drosophila melanogaster sirtuin 1 (Sirt1), mRNA.

Price & Availability↑

CloneID	OFa14599
Clone ID Related Accession (Same CDS sequence)	NM_058003.4
Accession Version	NM_058003.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sirtuin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058003.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGAAA ATTACGAGGA AATTCGCCTG GGCCACATTA GGTCTAAAGA TCTGGGCAAC 
CAGGTGCCAG ACACTACGCA ATTCTATCCG CCAACTAAGT TTGATTTTGG CGCGGAAATT 
CTGGCCTCAA CGTCAACAGA GGCAGAGGCA GAGGCAGAAG CAACAGCAAC AACCACAGAA 
CCAGCAACAA GCGAACTTGC TGGCAAAGCA AATGGTGAAA TCAAAACAAA AACATTGGCT 
GCCAGGGAAG AACAAGAGAT TGGCGCCAAT TTGGAGCATA AAACCAAAAA TCCCACAAAG 
TCAATGGGCG AGGATGAAGA TGACGAGGAG GAGGAGGAAG AGGACGATGA GGAGGAGGAG 
GAGGACGACG AGGAGGGAAT CACCGGAACG AGCAACGAGG ATGAGGACTC CAGCTCAAAT 
TGCTCCTCAT CCGTGGAACC CGACTGGAAG CTGCGCTGGT TGCAACGAGA ATTTTACACA 
GGTCGTGTGC CGCGCCAGGT TATTGCCAGC ATTATGCCGC ATTTCGCCAC CGGCCTGGCG 
GGCGACACCG ACGACTCCGT GCTGTGGGAC TATTTGGCCC ACCTGTTGAA CGAGCCGAAG 
CGGCGCAACA AGCTGGCCTC AGTGAACACC TTCGACGATG TCATCAGTTT GGTCAAGAAA 
TCACAGAAGA TCATTGTGCT AACGGGAGCC GGAGTATCCG TCTCCTGCGG CATTCCGGAC 
TTCCGGTCCA CCAATGGCAT ATATGCGCGA TTGGCCCATG ATTTTCCCGA TCTGCCCGAT 
CCGCAGGCCA TGTTTGATAT CAACTACTTC AAGAGGGATC CACGACCGTT CTACAAGTTT 
GCCCGCGAGA TATATCCCGG CGAGTTTCAG CCCTCACCCT GCCATCGTTT CATCAAAATG 
CTGGAGACCA AGGGCAAACT GTTGCGCAAC TACACACAGA ACATCGACAC CCTCGAGCGG 
GTGGCAGGCA TTCAGCGAGT AATCGAGTGT CACGGCTCCT TTTCAACGGC CTCGTGCACC 
AAGTGTCGTT TCAAGTGCAA CGCTGACGCC CTGCGGGCGG ACATATTTGC CCAGCGAATT 
CCGGTGTGCC CGCAGTGCCA GCCCAATAAG GAGCAGAGCG TGGATGCCTC GGTGGCCGTT 
ACTGAGGAGG AGCTGCGCCA ACTGGTCGAG AACGGCATCA TGAAGCCGGA TATCGTCTTT 
TTCGGCGAGG GACTGCCGGA TGAGTACCAC ACGGTCATGG CCACCGACAA GGACGTGTGC 
GATCTACTGA TCGTGATCGG CTCCTCGCTG AAGGTCCGAC CTGTGGCCCA CATTCCCAGC 
AGCATACCGG CCACGGTGCC GCAGATTCTT ATCAATCGCG AGCAGCTGCA TCACCTTAAG 
TTCGATGTGG AGCTGCTGGG CGACTCCGAT GTGATCATCA ACCAGATTTG CCACCGGTTG 
TCGGACAACG ATGATTGCTG GCGGCAGCTG TGCTGCGATG AGTCAGTGCT TACCGAAAGC 
AAGGAGCTAA TGCCTCCGGA GCACTCTAAT CACCACCTCC ATCATCATCT ACTTCACCAC 
CGCCACTGCA GTTCAGAGAG CGAGCGACAG TCGCAACTGG ACACGGATAC GCAGTCTATT 
AAATCAAATA GTTCGGCGGA CTACATACTA GGATCAGCTG GCACCTGCTC GGATAGTGGA 
TTTGAGTCAT CTACTTTTAG CTGTGGAAAG CGTTCCACTG CCGCCGAAGC GGCAGCCATC 
GAACGTATTA AAACAGACAT ACTGGTTGAG CTGAACGAGA CCACAGCCCT AAGTTGCGAT 
CGTCTGGGCC TGGAAGGCCC TCAGACAACG GTGGAGAGCT ATCGCCATCT TTCCATTGAT 
TCCTCCAAGG ATAGCGGCAT CGAGCAGTGC GACAACGAAG CCACGCCTAG CTACGTGCGA 
CCCAGCAATC TTGTTCAGGA GACCAAGACA GTGGCGCCCA GCCTGACGCC CATTCCACAA 
CAGAGGGGAA AGCGACAGAC AGCAGCCGAG CGTCTGCAGC CTGGAACATT CTATTCGCAC 
ACCAACAACT ATTCGTATGT GTTTCCAGGA GCCCAGGTAT TCTGGGACAA CGATTACAGC 
GATGATGATG ACGAAGAAGA GGAAAGATCA CACAATAGAC ACAGTGATCT CTTTGGCAAT 
GTGGGGCACA ATTATAAGGA TGATGATGAG GATGCATGTG ATCTGAACGC CGTTCCATTG 
TCACCATTGC TACCGCCTTC ACTGGAGGCT CACATAGTCA CCGATATAGT GAATGGATCC 
AACGAACCGC TGCCCAACAG CAGTCCCGGC CAGAAAAGAA CCGCCTGCAT TATAGAACAG 
CAGCCAACGC CCGCTATTGA AACGGAAATT CCCCCACTAA AGAAGCGGCG ACCAAGTGAG 
GAAAATAAGC AGCAGACCCA AATAGAAAGA TCTGAGGAGA GTCCGCCTCC AGGACAGTTA 
GCAGCAGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477351.1
CDS	428..2899
Translation	MMENYEEIRLGHIRSKDLGNQVPDTTQFYPPTKFDFGAEILASTSTEAEAEAEATATTTEPATSELAGKANGEIKTKTLAAREEQEIGANLEHKTKNPTKSMGEDEDDEEEEEEDDEEEEEDDEEGITGTSNEDEDSSSNCSSSVEPDWKLRWLQREFYTGRVPRQVIASIMPHFATGLAGDTDDSVLWDYLAHLLNEPKRRNKLASVNTFDDVISLVKKSQKIIVLTGAGVSVSCGIPDFRSTNGIYARLAHDFPDLPDPQAMFDINYFKRDPRPFYKFAREIYPGEFQPSPCHRFIKMLETKGKLLRNYTQNIDTLERVAGIQRVIECHGSFSTASCTKCRFKCNADALRADIFAQRIPVCPQCQPNKEQSVDASVAVTEEELRQLVENGIMKPDIVFFGEGLPDEYHTVMATDKDVCDLLIVIGSSLKVRPVAHIPSSIPATVPQILINREQLHHLKFDVELLGDSDVIINQICHRLSDNDDCWRQLCCDESVLTESKELMPPEHSNHHLHHHLLHHRHCSSESERQSQLDTDTQSIKSNSSADYILGSAGTCSDSGFESSTFSCGKRSTAAEAAAIERIKTDILVELNETTALSCDRLGLEGPQTTVESYRHLSIDSSKDSGIEQCDNEATPSYVRPSNLVQETKTVAPSLTPIPQQRGKRQTAAERLQPGTFYSHTNNYSYVFPGAQVFWDNDYSDDDDEEEERSHNRHSDLFGNVGHNYKDDDEDACDLNAVPLSPLLPPSLEAHIVTDIVNGSNEPLPNSSPGQKRTACIIEQQPTPAIETEIPPLKKRRPSEENKQQTQIERSEESPPPGQLAAV

Target ORF information:

RefSeq Version	NM_058003.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sirtuin 1 (Sirt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058003.4

ORF Insert Sequence:

ATGATGGAAA ATTACGAGGA AATTCGCCTG GGCCACATTA GGTCTAAAGA TCTGGGCAAC 
CAGGTGCCAG ACACTACGCA ATTCTATCCG CCAACTAAGT TTGATTTTGG CGCGGAAATT 
CTGGCCTCAA CGTCAACAGA GGCAGAGGCA GAGGCAGAAG CAACAGCAAC AACCACAGAA 
CCAGCAACAA GCGAACTTGC TGGCAAAGCA AATGGTGAAA TCAAAACAAA AACATTGGCT 
GCCAGGGAAG AACAAGAGAT TGGCGCCAAT TTGGAGCATA AAACCAAAAA TCCCACAAAG 
TCAATGGGCG AGGATGAAGA TGACGAGGAG GAGGAGGAAG AGGACGATGA GGAGGAGGAG 
GAGGACGACG AGGAGGGAAT CACCGGAACG AGCAACGAGG ATGAGGACTC CAGCTCAAAT 
TGCTCCTCAT CCGTGGAACC CGACTGGAAG CTGCGCTGGT TGCAACGAGA ATTTTACACA 
GGTCGTGTGC CGCGCCAGGT TATTGCCAGC ATTATGCCGC ATTTCGCCAC CGGCCTGGCG 
GGCGACACCG ACGACTCCGT GCTGTGGGAC TATTTGGCCC ACCTGTTGAA CGAGCCGAAG 
CGGCGCAACA AGCTGGCCTC AGTGAACACC TTCGACGATG TCATCAGTTT GGTCAAGAAA 
TCACAGAAGA TCATTGTGCT AACGGGAGCC GGAGTATCCG TCTCCTGCGG CATTCCGGAC 
TTCCGGTCCA CCAATGGCAT ATATGCGCGA TTGGCCCATG ATTTTCCCGA TCTGCCCGAT 
CCGCAGGCCA TGTTTGATAT CAACTACTTC AAGAGGGATC CACGACCGTT CTACAAGTTT 
GCCCGCGAGA TATATCCCGG CGAGTTTCAG CCCTCACCCT GCCATCGTTT CATCAAAATG 
CTGGAGACCA AGGGCAAACT GTTGCGCAAC TACACACAGA ACATCGACAC CCTCGAGCGG 
GTGGCAGGCA TTCAGCGAGT AATCGAGTGT CACGGCTCCT TTTCAACGGC CTCGTGCACC 
AAGTGTCGTT TCAAGTGCAA CGCTGACGCC CTGCGGGCGG ACATATTTGC CCAGCGAATT 
CCGGTGTGCC CGCAGTGCCA GCCCAATAAG GAGCAGAGCG TGGATGCCTC GGTGGCCGTT 
ACTGAGGAGG AGCTGCGCCA ACTGGTCGAG AACGGCATCA TGAAGCCGGA TATCGTCTTT 
TTCGGCGAGG GACTGCCGGA TGAGTACCAC ACGGTCATGG CCACCGACAA GGACGTGTGC 
GATCTACTGA TCGTGATCGG CTCCTCGCTG AAGGTCCGAC CTGTGGCCCA CATTCCCAGC 
AGCATACCGG CCACGGTGCC GCAGATTCTT ATCAATCGCG AGCAGCTGCA TCACCTTAAG 
TTCGATGTGG AGCTGCTGGG CGACTCCGAT GTGATCATCA ACCAGATTTG CCACCGGTTG 
TCGGACAACG ATGATTGCTG GCGGCAGCTG TGCTGCGATG AGTCAGTGCT TACCGAAAGC 
AAGGAGCTAA TGCCTCCGGA GCACTCTAAT CACCACCTCC ATCATCATCT ACTTCACCAC 
CGCCACTGCA GTTCAGAGAG CGAGCGACAG TCGCAACTGG ACACGGATAC GCAGTCTATT 
AAATCAAATA GTTCGGCGGA CTACATACTA GGATCAGCTG GCACCTGCTC GGATAGTGGA 
TTTGAGTCAT CTACTTTTAG CTGTGGAAAG CGTTCCACTG CCGCCGAAGC GGCAGCCATC 
GAACGTATTA AAACAGACAT ACTGGTTGAG CTGAACGAGA CCACAGCCCT AAGTTGCGAT 
CGTCTGGGCC TGGAAGGCCC TCAGACAACG GTGGAGAGCT ATCGCCATCT TTCCATTGAT 
TCCTCCAAGG ATAGCGGCAT CGAGCAGTGC GACAACGAAG CCACGCCTAG CTACGTGCGA 
CCCAGCAATC TTGTTCAGGA GACCAAGACA GTGGCGCCCA GCCTGACGCC CATTCCACAA 
CAGAGGGGAA AGCGACAGAC AGCAGCCGAG CGTCTGCAGC CTGGAACATT CTATTCGCAC 
ACCAACAACT ATTCGTATGT GTTTCCAGGA GCCCAGGTAT TCTGGGACAA CGATTACAGC 
GATGATGATG ACGAAGAAGA GGAAAGATCA CACAATAGAC ACAGTGATCT CTTTGGCAAT 
GTGGGGCACA ATTATAAGGA TGATGATGAG GATGCATGTG ATCTGAACGC CGTTCCATTG 
TCACCATTGC TACCGCCTTC ACTGGAGGCT CACATAGTCA CCGATATAGT GAATGGATCC 
AACGAACCGC TGCCCAACAG CAGTCCCGGC CAGAAAAGAA CCGCCTGCAT TATAGAACAG 
CAGCCAACGC CCGCTATTGA AACGGAAATT CCCCCACTAA AGAAGCGGCG ACCAAGTGAG 
GAAAATAAGC AGCAGACCCA AATAGAAAGA TCTGAGGAGA GTCCGCCTCC AGGACAGTTA 
GCAGCAGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sirt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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