Vha100-5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha100-5
Entrez Gene ID	34587
Full Name	Vacuolar H[+] ATPase 100kD subunit 5
Synonyms	CG12602,Dmel\CG12602,vha100-5
General protein information	Preferred Names Vacuolar H[+] ATPase 100kD subunit 5 Names CG12602 gene product from transcript CG12602-RB CG12602-PA CG12602-PB Vha100-5-PA Vha100-5-PB vacuolar H[+] ATPase 100kD subunit 5 vacuolar H[+] ATPase subunit 100-5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33A1-33A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273459.1	NP_001260388.1	vacuolar H[+] ATPase 100kD subunit 5, isoform B
NM_135671.2	NP_609515.1	vacuolar H[+] ATPase 100kD subunit 5, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha100-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha100-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14463	NM_135671.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant A (Vha100-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14463	NM_001273459.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant B (Vha100-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha100-5 ( NM_001273459.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260388.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant B (Vha100-5), mRNA.

Price & Availability↑

CloneID	OFa14463
Clone ID Related Accession (Same CDS sequence)	NM_001273459.1 , NM_135671.2
Accession Version	NM_001273459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGACA TGTTCCGCAG CGAGAAGATG GCCCTGTGCC AGCTGTTCAT CCAGCCGGAG 
GCAGCCTACG CCTCCATTGC CGAGCTGGGC GAAAAAGGAT GTGTCCAGTT TCGCGATCTG 
AACGAGGAGG TCAGTGCTTT CCAGCGAAAG TACGTCAACG AGGTCAGGAG ATGTGATGAC 
ATGGAGCGCC GCCTCCGGTA CGTGGAGTCC GAGATGAAGA AGGACGAGGT AAAGCTGCCA 
GTCCTGCGAC CGGAGGAGGA GCCCATTGCT CCCAATCCCC GCGAGATCGT AGACCTTGAG 
GCTCAGCTGG AGAAGACCGA CAATGAACTG CGCGAAATGT CCGCCAATGG AGCGAGTTTG 
GATGCCAACT TCCGGCACAT GCAAGAACTC AAATACGTGC TAGAGAACAC CGAGGGCTTC 
TTCTCCGATC AGGAAGTCAT TAATCTGGAC GTCAACCGAA AACTGGACCC CGAGGATCCG 
GCCAATTTGC CAGGAGCCGC CCAGCGGGGT CAACTGGCCT TTGTTGCTGG TGTCATCAAG 
CTGGAGCGAT TCTTCAGCTT TGAGCGCATG CTGTGGCGCA TTTCCAGAGG AAATATCTTC 
CTGCGGAGAG CCGACATTGA TGGGCTCGTC GCCGATGAGG AAACTGGAAG GCCTGTACTA 
AAGACCGTCT TCGTGGCCTT CTTCCAGGGA GAGCAGCTGA AGCAAAGAAT CAAGAAAGTC 
TGCACGGGCT ATCATGCCGC CGTCTATCCC TGTCCCAGTT CGCATGCCGA GCGAAAAGAG 
ATGATCAAGG ATGTGAATGT CCGTCTGGAG GACCTCAAGT TGGTCCTCAG CCAGAGCGCC 
GATCATCGCA GCCGGGTTTT GAACTCAGCC TCTAAGCATT TGCCACGCTG GTCGATTATG 
GTGCGCAAAA TGAAGGCTAT CTATCACATT CTGAACTTCT TCAATCCCGA TGTGACGGGC 
AAGTGTCTGA TTGGCGAGGG TTGGGTGCCC ACCAACGATA TATCCACGGT TCAGGATGCA 
CTAGCTCGAG CCTCGAAGAT CTCGGAAAGT TCCATACCGG CATTCATGAA TGTCATCGAG 
ACGAACGAAA TGCCGCCGAC TTACACGAGA ACCAATAAAT TCACCAATGG CTTCCAGAAC 
CTGGTTGACT CGTATGGAAT GGCCTCATAT AGAGAAGTGA ATCCGGCTCT TTACGCCTGC 
ATCACATTCC CCTTTCTGTT CGCCGTGATG TTCGGTGACT TGGGACACGG ACTGATCCTG 
CTGCTCTTCG CCTCCTGGCT AATCATCAAG GAAAAGCAAT TGTCTTCGAT CAAGGAGGAG 
ATCTTCAACA TATTCTTCGG CGGCCGATAC ATCATCTTCC TCATGGGAAT TTTCTCCATT 
TACACCGGAT TCATTTACAA CGATGTGTTC TCAAAGTCCA TGAATATATT CGGCTCCGCT 
TGGCACATGA ACTATACAAG GGACGTGGTT GAGGATGAAA ACCTTAAATA TATCACCCTC 
AGGCCGAACG ATACTGTTTA CAAGACATAT CCCTTCGGCA TGGATCCCAT TTGGCAACTG 
GCCGACAACA AGATCATCTT TCTAAACACC TTTAAGATGA AGCTGTCGAT CATTGTGGGT 
GTGATCCACA TGATCTTCGG CGTCTCCATG TCGGTGGTTA ACTTTGCCTA CTACAAGAAG 
TACGCAAGTA TTTTCCTAGA GTTCCTGCCT CAAGTCCTGT TCCTTCTCCT GTTGTTCGGT 
TACATGGTCT TCATGATGTT CTTCAAGTGG GTCGTTTACA ACGACACCGT TGAAGGACCA 
CTTTCACCGG CTTGTGCTCC TTCTATCCTG ATCCTGTTCA TCAACATGAT ACTGCAGGGC 
AGCCAGGACA CTCCTGAGCC CTGCAAGGAG TTCATGTTCG ACGGTCAGAA GAGCATCCAA 
CAGGTCTTTG TCGTCGTTGC CATCATTTGC ATTCCTTGGA TGCTTTTGGG CAAGCCTCTG 
TACATCATGA TCAAACGCAA GACGAACGGA GCTCCCCCAC CAAAACCACA AAGCGGTGGA 
GGGGAGGGTC ATGGAGAGGA CGATGAGATG GGCGAGATCT TCATCCATCA GGCCATCCAC 
ACCATCGAAT ATGTCCTGAG TACGGTCTCC CACACGGCCT CATACCTCCG ATTGTGGGCC 
TTATCCCTGG CTCACGCACA GCTCTCCGAA GTACTGTGGA ACATGGTGTT TTCCATGGGA 
TTCAAGTACG ACAGCTATAT TGGCGGCATT CTCATCTACG TATTCTTTGG AGCCTGGGCG 
TTGCTCACAG TCGGTATCCT GGTGCTCATC GAAGGACTCT CGGCCTTCTT GCACACACTG 
CGTTTGCACT GGGTTGAGTT CATGAGCAAG TTCTACGAGG GCGCTGGTTA CGCCTTTGAG 
CCTTTTGCCT TTAAGACCAT TTTGGATGTC AGCGAGGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260388.1
CDS	111..2555
Translation	MGDMFRSEKMALCQLFIQPEAAYASIAELGEKGCVQFRDLNEEVSAFQRKYVNEVRRCDDMERRLRYVESEMKKDEVKLPVLRPEEEPIAPNPREIVDLEAQLEKTDNELREMSANGASLDANFRHMQELKYVLENTEGFFSDQEVINLDVNRKLDPEDPANLPGAAQRGQLAFVAGVIKLERFFSFERMLWRISRGNIFLRRADIDGLVADEETGRPVLKTVFVAFFQGEQLKQRIKKVCTGYHAAVYPCPSSHAERKEMIKDVNVRLEDLKLVLSQSADHRSRVLNSASKHLPRWSIMVRKMKAIYHILNFFNPDVTGKCLIGEGWVPTNDISTVQDALARASKISESSIPAFMNVIETNEMPPTYTRTNKFTNGFQNLVDSYGMASYREVNPALYACITFPFLFAVMFGDLGHGLILLLFASWLIIKEKQLSSIKEEIFNIFFGGRYIIFLMGIFSIYTGFIYNDVFSKSMNIFGSAWHMNYTRDVVEDENLKYITLRPNDTVYKTYPFGMDPIWQLADNKIIFLNTFKMKLSIIVGVIHMIFGVSMSVVNFAYYKKYASIFLEFLPQVLFLLLLFGYMVFMMFFKWVVYNDTVEGPLSPACAPSILILFINMILQGSQDTPEPCKEFMFDGQKSIQQVFVVVAIICIPWMLLGKPLYIMIKRKTNGAPPPKPQSGGGEGHGEDDEMGEIFIHQAIHTIEYVLSTVSHTASYLRLWALSLAHAQLSEVLWNMVFSMGFKYDSYIGGILIYVFFGAWALLTVGILVLIEGLSAFLHTLRLHWVEFMSKFYEGAGYAFEPFAFKTILDVSEDD

Target ORF information:

RefSeq Version	NM_001273459.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant B (Vha100-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273459.1

ORF Insert Sequence:

ATGGGTGACA TGTTCCGCAG CGAGAAGATG GCCCTGTGCC AGCTGTTCAT CCAGCCGGAG 
GCAGCCTACG CCTCCATTGC CGAGCTGGGC GAAAAAGGAT GTGTCCAGTT TCGCGATCTG 
AACGAGGAGG TCAGTGCTTT CCAGCGAAAG TACGTCAACG AGGTCAGGAG ATGTGATGAC 
ATGGAGCGCC GCCTCCGGTA CGTGGAGTCC GAGATGAAGA AGGACGAGGT AAAGCTGCCA 
GTCCTGCGAC CGGAGGAGGA GCCCATTGCT CCCAATCCCC GCGAGATCGT AGACCTTGAG 
GCTCAGCTGG AGAAGACCGA CAATGAACTG CGCGAAATGT CCGCCAATGG AGCGAGTTTG 
GATGCCAACT TCCGGCACAT GCAAGAACTC AAATACGTGC TAGAGAACAC CGAGGGCTTC 
TTCTCCGATC AGGAAGTCAT TAATCTGGAC GTCAACCGAA AACTGGACCC CGAGGATCCG 
GCCAATTTGC CAGGAGCCGC CCAGCGGGGT CAACTGGCCT TTGTTGCTGG TGTCATCAAG 
CTGGAGCGAT TCTTCAGCTT TGAGCGCATG CTGTGGCGCA TTTCCAGAGG AAATATCTTC 
CTGCGGAGAG CCGACATTGA TGGGCTCGTC GCCGATGAGG AAACTGGAAG GCCTGTACTA 
AAGACCGTCT TCGTGGCCTT CTTCCAGGGA GAGCAGCTGA AGCAAAGAAT CAAGAAAGTC 
TGCACGGGCT ATCATGCCGC CGTCTATCCC TGTCCCAGTT CGCATGCCGA GCGAAAAGAG 
ATGATCAAGG ATGTGAATGT CCGTCTGGAG GACCTCAAGT TGGTCCTCAG CCAGAGCGCC 
GATCATCGCA GCCGGGTTTT GAACTCAGCC TCTAAGCATT TGCCACGCTG GTCGATTATG 
GTGCGCAAAA TGAAGGCTAT CTATCACATT CTGAACTTCT TCAATCCCGA TGTGACGGGC 
AAGTGTCTGA TTGGCGAGGG TTGGGTGCCC ACCAACGATA TATCCACGGT TCAGGATGCA 
CTAGCTCGAG CCTCGAAGAT CTCGGAAAGT TCCATACCGG CATTCATGAA TGTCATCGAG 
ACGAACGAAA TGCCGCCGAC TTACACGAGA ACCAATAAAT TCACCAATGG CTTCCAGAAC 
CTGGTTGACT CGTATGGAAT GGCCTCATAT AGAGAAGTGA ATCCGGCTCT TTACGCCTGC 
ATCACATTCC CCTTTCTGTT CGCCGTGATG TTCGGTGACT TGGGACACGG ACTGATCCTG 
CTGCTCTTCG CCTCCTGGCT AATCATCAAG GAAAAGCAAT TGTCTTCGAT CAAGGAGGAG 
ATCTTCAACA TATTCTTCGG CGGCCGATAC ATCATCTTCC TCATGGGAAT TTTCTCCATT 
TACACCGGAT TCATTTACAA CGATGTGTTC TCAAAGTCCA TGAATATATT CGGCTCCGCT 
TGGCACATGA ACTATACAAG GGACGTGGTT GAGGATGAAA ACCTTAAATA TATCACCCTC 
AGGCCGAACG ATACTGTTTA CAAGACATAT CCCTTCGGCA TGGATCCCAT TTGGCAACTG 
GCCGACAACA AGATCATCTT TCTAAACACC TTTAAGATGA AGCTGTCGAT CATTGTGGGT 
GTGATCCACA TGATCTTCGG CGTCTCCATG TCGGTGGTTA ACTTTGCCTA CTACAAGAAG 
TACGCAAGTA TTTTCCTAGA GTTCCTGCCT CAAGTCCTGT TCCTTCTCCT GTTGTTCGGT 
TACATGGTCT TCATGATGTT CTTCAAGTGG GTCGTTTACA ACGACACCGT TGAAGGACCA 
CTTTCACCGG CTTGTGCTCC TTCTATCCTG ATCCTGTTCA TCAACATGAT ACTGCAGGGC 
AGCCAGGACA CTCCTGAGCC CTGCAAGGAG TTCATGTTCG ACGGTCAGAA GAGCATCCAA 
CAGGTCTTTG TCGTCGTTGC CATCATTTGC ATTCCTTGGA TGCTTTTGGG CAAGCCTCTG 
TACATCATGA TCAAACGCAA GACGAACGGA GCTCCCCCAC CAAAACCACA AAGCGGTGGA 
GGGGAGGGTC ATGGAGAGGA CGATGAGATG GGCGAGATCT TCATCCATCA GGCCATCCAC 
ACCATCGAAT ATGTCCTGAG TACGGTCTCC CACACGGCCT CATACCTCCG ATTGTGGGCC 
TTATCCCTGG CTCACGCACA GCTCTCCGAA GTACTGTGGA ACATGGTGTT TTCCATGGGA 
TTCAAGTACG ACAGCTATAT TGGCGGCATT CTCATCTACG TATTCTTTGG AGCCTGGGCG 
TTGCTCACAG TCGGTATCCT GGTGCTCATC GAAGGACTCT CGGCCTTCTT GCACACACTG 
CGTTTGCACT GGGTTGAGTT CATGAGCAAG TTCTACGAGG GCGCTGGTTA CGCCTTTGAG 
CCTTTTGCCT TTAAGACCAT TTTGGATGTC AGCGAGGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-5 ( NM_135671.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609515.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant A (Vha100-5), mRNA.

Price & Availability↑

CloneID	OFa14463
Clone ID Related Accession (Same CDS sequence)	NM_001273459.1 , NM_135671.2
Accession Version	NM_135671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135671.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGACA TGTTCCGCAG CGAGAAGATG GCCCTGTGCC AGCTGTTCAT CCAGCCGGAG 
GCAGCCTACG CCTCCATTGC CGAGCTGGGC GAAAAAGGAT GTGTCCAGTT TCGCGATCTG 
AACGAGGAGG TCAGTGCTTT CCAGCGAAAG TACGTCAACG AGGTCAGGAG ATGTGATGAC 
ATGGAGCGCC GCCTCCGGTA CGTGGAGTCC GAGATGAAGA AGGACGAGGT AAAGCTGCCA 
GTCCTGCGAC CGGAGGAGGA GCCCATTGCT CCCAATCCCC GCGAGATCGT AGACCTTGAG 
GCTCAGCTGG AGAAGACCGA CAATGAACTG CGCGAAATGT CCGCCAATGG AGCGAGTTTG 
GATGCCAACT TCCGGCACAT GCAAGAACTC AAATACGTGC TAGAGAACAC CGAGGGCTTC 
TTCTCCGATC AGGAAGTCAT TAATCTGGAC GTCAACCGAA AACTGGACCC CGAGGATCCG 
GCCAATTTGC CAGGAGCCGC CCAGCGGGGT CAACTGGCCT TTGTTGCTGG TGTCATCAAG 
CTGGAGCGAT TCTTCAGCTT TGAGCGCATG CTGTGGCGCA TTTCCAGAGG AAATATCTTC 
CTGCGGAGAG CCGACATTGA TGGGCTCGTC GCCGATGAGG AAACTGGAAG GCCTGTACTA 
AAGACCGTCT TCGTGGCCTT CTTCCAGGGA GAGCAGCTGA AGCAAAGAAT CAAGAAAGTC 
TGCACGGGCT ATCATGCCGC CGTCTATCCC TGTCCCAGTT CGCATGCCGA GCGAAAAGAG 
ATGATCAAGG ATGTGAATGT CCGTCTGGAG GACCTCAAGT TGGTCCTCAG CCAGAGCGCC 
GATCATCGCA GCCGGGTTTT GAACTCAGCC TCTAAGCATT TGCCACGCTG GTCGATTATG 
GTGCGCAAAA TGAAGGCTAT CTATCACATT CTGAACTTCT TCAATCCCGA TGTGACGGGC 
AAGTGTCTGA TTGGCGAGGG TTGGGTGCCC ACCAACGATA TATCCACGGT TCAGGATGCA 
CTAGCTCGAG CCTCGAAGAT CTCGGAAAGT TCCATACCGG CATTCATGAA TGTCATCGAG 
ACGAACGAAA TGCCGCCGAC TTACACGAGA ACCAATAAAT TCACCAATGG CTTCCAGAAC 
CTGGTTGACT CGTATGGAAT GGCCTCATAT AGAGAAGTGA ATCCGGCTCT TTACGCCTGC 
ATCACATTCC CCTTTCTGTT CGCCGTGATG TTCGGTGACT TGGGACACGG ACTGATCCTG 
CTGCTCTTCG CCTCCTGGCT AATCATCAAG GAAAAGCAAT TGTCTTCGAT CAAGGAGGAG 
ATCTTCAACA TATTCTTCGG CGGCCGATAC ATCATCTTCC TCATGGGAAT TTTCTCCATT 
TACACCGGAT TCATTTACAA CGATGTGTTC TCAAAGTCCA TGAATATATT CGGCTCCGCT 
TGGCACATGA ACTATACAAG GGACGTGGTT GAGGATGAAA ACCTTAAATA TATCACCCTC 
AGGCCGAACG ATACTGTTTA CAAGACATAT CCCTTCGGCA TGGATCCCAT TTGGCAACTG 
GCCGACAACA AGATCATCTT TCTAAACACC TTTAAGATGA AGCTGTCGAT CATTGTGGGT 
GTGATCCACA TGATCTTCGG CGTCTCCATG TCGGTGGTTA ACTTTGCCTA CTACAAGAAG 
TACGCAAGTA TTTTCCTAGA GTTCCTGCCT CAAGTCCTGT TCCTTCTCCT GTTGTTCGGT 
TACATGGTCT TCATGATGTT CTTCAAGTGG GTCGTTTACA ACGACACCGT TGAAGGACCA 
CTTTCACCGG CTTGTGCTCC TTCTATCCTG ATCCTGTTCA TCAACATGAT ACTGCAGGGC 
AGCCAGGACA CTCCTGAGCC CTGCAAGGAG TTCATGTTCG ACGGTCAGAA GAGCATCCAA 
CAGGTCTTTG TCGTCGTTGC CATCATTTGC ATTCCTTGGA TGCTTTTGGG CAAGCCTCTG 
TACATCATGA TCAAACGCAA GACGAACGGA GCTCCCCCAC CAAAACCACA AAGCGGTGGA 
GGGGAGGGTC ATGGAGAGGA CGATGAGATG GGCGAGATCT TCATCCATCA GGCCATCCAC 
ACCATCGAAT ATGTCCTGAG TACGGTCTCC CACACGGCCT CATACCTCCG ATTGTGGGCC 
TTATCCCTGG CTCACGCACA GCTCTCCGAA GTACTGTGGA ACATGGTGTT TTCCATGGGA 
TTCAAGTACG ACAGCTATAT TGGCGGCATT CTCATCTACG TATTCTTTGG AGCCTGGGCG 
TTGCTCACAG TCGGTATCCT GGTGCTCATC GAAGGACTCT CGGCCTTCTT GCACACACTG 
CGTTTGCACT GGGTTGAGTT CATGAGCAAG TTCTACGAGG GCGCTGGTTA CGCCTTTGAG 
CCTTTTGCCT TTAAGACCAT TTTGGATGTC AGCGAGGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609515.1
CDS	111..2555
Translation	MGDMFRSEKMALCQLFIQPEAAYASIAELGEKGCVQFRDLNEEVSAFQRKYVNEVRRCDDMERRLRYVESEMKKDEVKLPVLRPEEEPIAPNPREIVDLEAQLEKTDNELREMSANGASLDANFRHMQELKYVLENTEGFFSDQEVINLDVNRKLDPEDPANLPGAAQRGQLAFVAGVIKLERFFSFERMLWRISRGNIFLRRADIDGLVADEETGRPVLKTVFVAFFQGEQLKQRIKKVCTGYHAAVYPCPSSHAERKEMIKDVNVRLEDLKLVLSQSADHRSRVLNSASKHLPRWSIMVRKMKAIYHILNFFNPDVTGKCLIGEGWVPTNDISTVQDALARASKISESSIPAFMNVIETNEMPPTYTRTNKFTNGFQNLVDSYGMASYREVNPALYACITFPFLFAVMFGDLGHGLILLLFASWLIIKEKQLSSIKEEIFNIFFGGRYIIFLMGIFSIYTGFIYNDVFSKSMNIFGSAWHMNYTRDVVEDENLKYITLRPNDTVYKTYPFGMDPIWQLADNKIIFLNTFKMKLSIIVGVIHMIFGVSMSVVNFAYYKKYASIFLEFLPQVLFLLLLFGYMVFMMFFKWVVYNDTVEGPLSPACAPSILILFINMILQGSQDTPEPCKEFMFDGQKSIQQVFVVVAIICIPWMLLGKPLYIMIKRKTNGAPPPKPQSGGGEGHGEDDEMGEIFIHQAIHTIEYVLSTVSHTASYLRLWALSLAHAQLSEVLWNMVFSMGFKYDSYIGGILIYVFFGAWALLTVGILVLIEGLSAFLHTLRLHWVEFMSKFYEGAGYAFEPFAFKTILDVSEDD

Target ORF information:

RefSeq Version	NM_135671.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 5, transcript variant A (Vha100-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135671.2

ORF Insert Sequence:

ATGGGTGACA TGTTCCGCAG CGAGAAGATG GCCCTGTGCC AGCTGTTCAT CCAGCCGGAG 
GCAGCCTACG CCTCCATTGC CGAGCTGGGC GAAAAAGGAT GTGTCCAGTT TCGCGATCTG 
AACGAGGAGG TCAGTGCTTT CCAGCGAAAG TACGTCAACG AGGTCAGGAG ATGTGATGAC 
ATGGAGCGCC GCCTCCGGTA CGTGGAGTCC GAGATGAAGA AGGACGAGGT AAAGCTGCCA 
GTCCTGCGAC CGGAGGAGGA GCCCATTGCT CCCAATCCCC GCGAGATCGT AGACCTTGAG 
GCTCAGCTGG AGAAGACCGA CAATGAACTG CGCGAAATGT CCGCCAATGG AGCGAGTTTG 
GATGCCAACT TCCGGCACAT GCAAGAACTC AAATACGTGC TAGAGAACAC CGAGGGCTTC 
TTCTCCGATC AGGAAGTCAT TAATCTGGAC GTCAACCGAA AACTGGACCC CGAGGATCCG 
GCCAATTTGC CAGGAGCCGC CCAGCGGGGT CAACTGGCCT TTGTTGCTGG TGTCATCAAG 
CTGGAGCGAT TCTTCAGCTT TGAGCGCATG CTGTGGCGCA TTTCCAGAGG AAATATCTTC 
CTGCGGAGAG CCGACATTGA TGGGCTCGTC GCCGATGAGG AAACTGGAAG GCCTGTACTA 
AAGACCGTCT TCGTGGCCTT CTTCCAGGGA GAGCAGCTGA AGCAAAGAAT CAAGAAAGTC 
TGCACGGGCT ATCATGCCGC CGTCTATCCC TGTCCCAGTT CGCATGCCGA GCGAAAAGAG 
ATGATCAAGG ATGTGAATGT CCGTCTGGAG GACCTCAAGT TGGTCCTCAG CCAGAGCGCC 
GATCATCGCA GCCGGGTTTT GAACTCAGCC TCTAAGCATT TGCCACGCTG GTCGATTATG 
GTGCGCAAAA TGAAGGCTAT CTATCACATT CTGAACTTCT TCAATCCCGA TGTGACGGGC 
AAGTGTCTGA TTGGCGAGGG TTGGGTGCCC ACCAACGATA TATCCACGGT TCAGGATGCA 
CTAGCTCGAG CCTCGAAGAT CTCGGAAAGT TCCATACCGG CATTCATGAA TGTCATCGAG 
ACGAACGAAA TGCCGCCGAC TTACACGAGA ACCAATAAAT TCACCAATGG CTTCCAGAAC 
CTGGTTGACT CGTATGGAAT GGCCTCATAT AGAGAAGTGA ATCCGGCTCT TTACGCCTGC 
ATCACATTCC CCTTTCTGTT CGCCGTGATG TTCGGTGACT TGGGACACGG ACTGATCCTG 
CTGCTCTTCG CCTCCTGGCT AATCATCAAG GAAAAGCAAT TGTCTTCGAT CAAGGAGGAG 
ATCTTCAACA TATTCTTCGG CGGCCGATAC ATCATCTTCC TCATGGGAAT TTTCTCCATT 
TACACCGGAT TCATTTACAA CGATGTGTTC TCAAAGTCCA TGAATATATT CGGCTCCGCT 
TGGCACATGA ACTATACAAG GGACGTGGTT GAGGATGAAA ACCTTAAATA TATCACCCTC 
AGGCCGAACG ATACTGTTTA CAAGACATAT CCCTTCGGCA TGGATCCCAT TTGGCAACTG 
GCCGACAACA AGATCATCTT TCTAAACACC TTTAAGATGA AGCTGTCGAT CATTGTGGGT 
GTGATCCACA TGATCTTCGG CGTCTCCATG TCGGTGGTTA ACTTTGCCTA CTACAAGAAG 
TACGCAAGTA TTTTCCTAGA GTTCCTGCCT CAAGTCCTGT TCCTTCTCCT GTTGTTCGGT 
TACATGGTCT TCATGATGTT CTTCAAGTGG GTCGTTTACA ACGACACCGT TGAAGGACCA 
CTTTCACCGG CTTGTGCTCC TTCTATCCTG ATCCTGTTCA TCAACATGAT ACTGCAGGGC 
AGCCAGGACA CTCCTGAGCC CTGCAAGGAG TTCATGTTCG ACGGTCAGAA GAGCATCCAA 
CAGGTCTTTG TCGTCGTTGC CATCATTTGC ATTCCTTGGA TGCTTTTGGG CAAGCCTCTG 
TACATCATGA TCAAACGCAA GACGAACGGA GCTCCCCCAC CAAAACCACA AAGCGGTGGA 
GGGGAGGGTC ATGGAGAGGA CGATGAGATG GGCGAGATCT TCATCCATCA GGCCATCCAC 
ACCATCGAAT ATGTCCTGAG TACGGTCTCC CACACGGCCT CATACCTCCG ATTGTGGGCC 
TTATCCCTGG CTCACGCACA GCTCTCCGAA GTACTGTGGA ACATGGTGTT TTCCATGGGA 
TTCAAGTACG ACAGCTATAT TGGCGGCATT CTCATCTACG TATTCTTTGG AGCCTGGGCG 
TTGCTCACAG TCGGTATCCT GGTGCTCATC GAAGGACTCT CGGCCTTCTT GCACACACTG 
CGTTTGCACT GGGTTGAGTT CATGAGCAAG TTCTACGAGG GCGCTGGTTA CGCCTTTGAG 
CCTTTTGCCT TTAAGACCAT TTTGGATGTC AGCGAGGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha100-5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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